Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32209.t1 | ABD32605.1 | 73.8 | 260 | 67 | 1 | 1 | 259 | 24 | 283 | 6.60E-93 | 350.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32209.t1 | Q2HTY2 | 73.8 | 260 | 67 | 1 | 1 | 259 | 24 | 283 | 4.8e-93 | 350.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049501 | MS.gene32209 | 0.820504 | 6.62E-53 | -1.69E-46 |
| MS.gene050237 | MS.gene32209 | 0.810788 | 9.68E-51 | -1.69E-46 |
| MS.gene050807 | MS.gene32209 | 0.803428 | 3.50E-49 | -1.69E-46 |
| MS.gene051265 | MS.gene32209 | 0.816793 | 4.60E-52 | -1.69E-46 |
| MS.gene052561 | MS.gene32209 | 0.934239 | 5.49E-96 | -1.69E-46 |
| MS.gene055778 | MS.gene32209 | 0.806962 | 6.38E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32209.t1 | MTR_4g091220 | 92.692 | 260 | 18 | 1 | 1 | 259 | 24 | 283 | 1.72e-132 | 375 |
| MS.gene32209.t1 | MTR_4g091220 | 92.913 | 254 | 17 | 1 | 1 | 253 | 24 | 277 | 1.50e-127 | 363 |
| MS.gene32209.t1 | MTR_2g011760 | 52.330 | 279 | 92 | 9 | 1 | 259 | 1 | 258 | 1.31e-73 | 225 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32209.t1 | AT4G21500 | 34.413 | 247 | 105 | 8 | 17 | 246 | 9 | 215 | 2.07e-14 | 70.5 |
Find 48 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGTTTCTCTTCTCCACATT+TGG | 0.202266 | 4.4:+22676650 | None:intergenic |
| AAAACAAGAAAACTGGAATA+TGG | 0.269894 | 4.4:-22676439 | MS.gene32209:CDS |
| TCAATTTCCTTTGGTTCAAC+TGG | 0.327808 | 4.4:+22676615 | None:intergenic |
| GGTGAATTCCGAAAAGAGTT+TGG | 0.330170 | 4.4:-22676559 | MS.gene32209:CDS |
| CTCGTCGGAGGATGAGTTTC+CGG | 0.351454 | 4.4:-22676361 | MS.gene32209:CDS |
| CAAGATGATGAAGAGGGATT+TGG | 0.357467 | 4.4:-22676259 | MS.gene32209:CDS |
| TTGATTGAGATAGCTTTAGA+TGG | 0.357877 | 4.4:-22676285 | MS.gene32209:CDS |
| TCTAGAGACTCAATTTCCTT+TGG | 0.372592 | 4.4:+22676606 | None:intergenic |
| CACTGGAGAGAAAACTATCT+TGG | 0.409754 | 4.4:+22676800 | None:intergenic |
| GAGCTATCCGAATCAGATTC+CGG | 0.439128 | 4.4:+22676381 | None:intergenic |
| AGTTTCCGGCGAAAGAGGAT+TGG | 0.443586 | 4.4:-22676347 | MS.gene32209:CDS |
| GGATGAGTTTCCGGCGAAAG+AGG | 0.443918 | 4.4:-22676352 | MS.gene32209:CDS |
| GGGAAGAAGAAGACAGAGAA+GGG | 0.445414 | 4.4:-22676308 | MS.gene32209:CDS |
| AAACAAGAAAACTGGAATAT+GGG | 0.452373 | 4.4:-22676438 | MS.gene32209:CDS |
| AAAATATCATCATGAACAAA+AGG | 0.457722 | 4.4:-22676724 | MS.gene32209:CDS |
| GTTTCCGGCGAAAGAGGATT+GGG | 0.460419 | 4.4:-22676346 | MS.gene32209:CDS |
| AGAAAAGATGAGGTAAATAG+AGG | 0.464575 | 4.4:+22676823 | None:intergenic |
| CTCCAGTGCTTATTCTCACC+GGG | 0.467412 | 4.4:-22676785 | MS.gene32209:CDS |
| TGGAAGCAAGATGATGAAGA+GGG | 0.469934 | 4.4:-22676265 | MS.gene32209:CDS |
| TCTCCAGTGCTTATTCTCAC+CGG | 0.472662 | 4.4:-22676786 | MS.gene32209:CDS |
| TTCGTTGTCACGATACTATC+CGG | 0.478563 | 4.4:-22676400 | MS.gene32209:CDS |
| GAGGAAAATCAAGGTGCAAA+AGG | 0.482289 | 4.4:+22676842 | None:intergenic |
| TTGGGACTCGCCGGAGGAGT+GGG | 0.488949 | 4.4:-22676328 | MS.gene32209:CDS |
| ATACTATCCGGAATCTGATT+CGG | 0.492058 | 4.4:-22676388 | MS.gene32209:CDS |
| ATGGAACGTGAGAGCACCAT+TGG | 0.500437 | 4.4:-22676514 | MS.gene32209:CDS |
| GAAAACAAAGCAACCAAATG+TGG | 0.507750 | 4.4:-22676663 | MS.gene32209:CDS |
| ATTGGGACTCGCCGGAGGAG+TGG | 0.510971 | 4.4:-22676329 | MS.gene32209:CDS |
| TCTTCGTATATTACCTCCAA+TGG | 0.512351 | 4.4:+22676498 | None:intergenic |
| TCTTCTTCTTCCCACTCCTC+CGG | 0.516810 | 4.4:+22676318 | None:intergenic |
| TGGGAAGAAGAAGACAGAGA+AGG | 0.524332 | 4.4:-22676309 | MS.gene32209:CDS |
| ATGGAAGCAAGATGATGAAG+AGG | 0.528161 | 4.4:-22676266 | MS.gene32209:CDS |
| TTGCTCACAATCCTCCGTCT+CGG | 0.556266 | 4.4:-22676756 | MS.gene32209:CDS |
| ATCCCGGTGAGAATAAGCAC+TGG | 0.563079 | 4.4:+22676783 | None:intergenic |
| ATCTGATTCGGATAGCTCGT+CGG | 0.574190 | 4.4:-22676376 | MS.gene32209:CDS |
| GATTGTGAGCAAGAGAATCC+CGG | 0.574606 | 4.4:+22676767 | None:intergenic |
| GAACGTGAGAGCACCATTGG+AGG | 0.580318 | 4.4:-22676511 | MS.gene32209:CDS |
| GAGTCCCAATCCTCTTTCGC+CGG | 0.581065 | 4.4:+22676342 | None:intergenic |
| AACATGTCCAGTTGAACCAA+AGG | 0.581805 | 4.4:-22676622 | MS.gene32209:CDS |
| GAAAGAGGATTGGGACTCGC+CGG | 0.585561 | 4.4:-22676337 | MS.gene32209:CDS |
| AGGTAAATAGAGGAAAATCA+AGG | 0.599703 | 4.4:+22676833 | None:intergenic |
| AGAGGATTGGGACTCGCCGG+AGG | 0.612220 | 4.4:-22676334 | MS.gene32209:CDS |
| TTTGATTTGCTCCGAGACGG+AGG | 0.615406 | 4.4:+22676745 | None:intergenic |
| TTAGTTGCACCTAATCTCAC+CGG | 0.625690 | 4.4:+22676170 | None:intergenic |
| ACTATCTTGGAGAAAAGATG+AGG | 0.649524 | 4.4:+22676813 | None:intergenic |
| GAAAACTCTTTGTACCTCGT+CGG | 0.660934 | 4.4:+22676192 | None:intergenic |
| TGATTCGGATAGCTCGTCGG+AGG | 0.685465 | 4.4:-22676373 | MS.gene32209:CDS |
| GTGACAATGCACGAAAACAG+AGG | 0.690366 | 4.4:+22676864 | None:intergenic |
| AGATTGATATTTCTCCGACG+AGG | 0.710192 | 4.4:-22676206 | MS.gene32209:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATATCATCATGAACAAA+AGG | - | chr4.4:22676373-22676392 | MS.gene32209:CDS | 20.0% |
| !! | ATATGAAGATGAAGAAAAAT+TGG | - | chr4.4:22676622-22676641 | MS.gene32209:CDS | 20.0% |
| !!! | AGATTTTGAAATAGATGAAA+TGG | + | chr4.4:22676195-22676214 | None:intergenic | 20.0% |
| ! | AAAACAAGAAAACTGGAATA+TGG | - | chr4.4:22676658-22676677 | MS.gene32209:CDS | 25.0% |
| ! | AAACAAGAAAACTGGAATAT+GGG | - | chr4.4:22676659-22676678 | MS.gene32209:CDS | 25.0% |
| ! | CAAATGAAAAACAAGAAAAC+TGG | - | chr4.4:22676651-22676670 | MS.gene32209:CDS | 25.0% |
| !!! | CAGTTTTCTTGTTTTTCATT+TGG | + | chr4.4:22676653-22676672 | None:intergenic | 25.0% |
| AGAAAAGATGAGGTAAATAG+AGG | + | chr4.4:22676277-22676296 | None:intergenic | 30.0% | |
| AGGTAAATAGAGGAAAATCA+AGG | + | chr4.4:22676267-22676286 | None:intergenic | 30.0% | |
| ! | ACTTGATTCCAAACTCTTTT+CGG | + | chr4.4:22676549-22676568 | None:intergenic | 30.0% |
| !! | TTGATTGAGATAGCTTTAGA+TGG | - | chr4.4:22676812-22676831 | MS.gene32209:CDS | 30.0% |
| ACTATCTTGGAGAAAAGATG+AGG | + | chr4.4:22676287-22676306 | None:intergenic | 35.0% | |
| ATACTATCCGGAATCTGATT+CGG | - | chr4.4:22676709-22676728 | MS.gene32209:CDS | 35.0% | |
| GAAAACAAAGCAACCAAATG+TGG | - | chr4.4:22676434-22676453 | MS.gene32209:CDS | 35.0% | |
| TCTAGAGACTCAATTTCCTT+TGG | + | chr4.4:22676494-22676513 | None:intergenic | 35.0% | |
| TCTTCGTATATTACCTCCAA+TGG | + | chr4.4:22676602-22676621 | None:intergenic | 35.0% | |
| ! | GTTTTCTTCTTTAGCGATGA+TGG | + | chr4.4:22676420-22676439 | None:intergenic | 35.0% |
| ! | TCAATTTCCTTTGGTTCAAC+TGG | + | chr4.4:22676485-22676504 | None:intergenic | 35.0% |
| !!! | ATTTTTGATTTGCTCCGAGA+CGG | + | chr4.4:22676358-22676377 | None:intergenic | 35.0% |
| AACATGTCCAGTTGAACCAA+AGG | - | chr4.4:22676475-22676494 | MS.gene32209:CDS | 40.0% | |
| AGATTGATATTTCTCCGACG+AGG | - | chr4.4:22676891-22676910 | MS.gene32209:CDS | 40.0% | |
| AGTGCACTGTTCAGAAAAAG+AGG | - | chr4.4:22676517-22676536 | MS.gene32209:CDS | 40.0% | |
| ATGGAAGCAAGATGATGAAG+AGG | - | chr4.4:22676831-22676850 | MS.gene32209:CDS | 40.0% | |
| CAAGATGATGAAGAGGGATT+TGG | - | chr4.4:22676838-22676857 | MS.gene32209:CDS | 40.0% | |
| CACTGGAGAGAAAACTATCT+TGG | + | chr4.4:22676300-22676319 | None:intergenic | 40.0% | |
| GAAAACTCTTTGTACCTCGT+CGG | + | chr4.4:22676908-22676927 | None:intergenic | 40.0% | |
| GAGGAAAATCAAGGTGCAAA+AGG | + | chr4.4:22676258-22676277 | None:intergenic | 40.0% | |
| GGTGAATTCCGAAAAGAGTT+TGG | - | chr4.4:22676538-22676557 | MS.gene32209:CDS | 40.0% | |
| TGGAAGCAAGATGATGAAGA+GGG | - | chr4.4:22676832-22676851 | MS.gene32209:CDS | 40.0% | |
| TTAGTTGCACCTAATCTCAC+CGG | + | chr4.4:22676930-22676949 | None:intergenic | 40.0% | |
| TTCGTTGTCACGATACTATC+CGG | - | chr4.4:22676697-22676716 | MS.gene32209:CDS | 40.0% | |
| ! | AAGAGTTTTCCGGTGAGATT+AGG | - | chr4.4:22676918-22676937 | MS.gene32209:CDS | 40.0% |
| ! | ACGAGGTACAAAGAGTTTTC+CGG | - | chr4.4:22676908-22676927 | MS.gene32209:CDS | 40.0% |
| ! | CTGTTTCTCTTCTCCACATT+TGG | + | chr4.4:22676450-22676469 | None:intergenic | 40.0% |
| !! | CAAGTTCGAGTTTTGTGGAA+TGG | - | chr4.4:22676564-22676583 | MS.gene32209:CDS | 40.0% |
| !! | GGAATCAAGTTCGAGTTTTG+TGG | - | chr4.4:22676559-22676578 | MS.gene32209:CDS | 40.0% |
| GAGCTATCCGAATCAGATTC+CGG | + | chr4.4:22676719-22676738 | None:intergenic | 45.0% | |
| GATTGTGAGCAAGAGAATCC+CGG | + | chr4.4:22676333-22676352 | None:intergenic | 45.0% | |
| GGGAAGAAGAAGACAGAGAA+GGG | - | chr4.4:22676789-22676808 | MS.gene32209:CDS | 45.0% | |
| GTGACAATGCACGAAAACAG+AGG | + | chr4.4:22676236-22676255 | None:intergenic | 45.0% | |
| TCTCCAGTGCTTATTCTCAC+CGG | - | chr4.4:22676311-22676330 | MS.gene32209:CDS | 45.0% | |
| TGGGAAGAAGAAGACAGAGA+AGG | - | chr4.4:22676788-22676807 | MS.gene32209:CDS | 45.0% | |
| !! | ATCTGATTCGGATAGCTCGT+CGG | - | chr4.4:22676721-22676740 | MS.gene32209:CDS | 45.0% |
| AGTTTCCGGCGAAAGAGGAT+TGG | - | chr4.4:22676750-22676769 | MS.gene32209:CDS | 50.0% | |
| ATCCCGGTGAGAATAAGCAC+TGG | + | chr4.4:22676317-22676336 | None:intergenic | 50.0% | |
| CTCCAGTGCTTATTCTCACC+GGG | - | chr4.4:22676312-22676331 | MS.gene32209:CDS | 50.0% | |
| GTTTCCGGCGAAAGAGGATT+GGG | - | chr4.4:22676751-22676770 | MS.gene32209:CDS | 50.0% | |
| TCTTCTTCTTCCCACTCCTC+CGG | + | chr4.4:22676782-22676801 | None:intergenic | 50.0% | |
| TTGCTCACAATCCTCCGTCT+CGG | - | chr4.4:22676341-22676360 | MS.gene32209:CDS | 50.0% | |
| !! | ATGGAACGTGAGAGCACCAT+TGG | - | chr4.4:22676583-22676602 | MS.gene32209:CDS | 50.0% |
| !! | TTTGATTTGCTCCGAGACGG+AGG | + | chr4.4:22676355-22676374 | None:intergenic | 50.0% |
| CTCGTCGGAGGATGAGTTTC+CGG | - | chr4.4:22676736-22676755 | MS.gene32209:CDS | 55.0% | |
| GAAAGAGGATTGGGACTCGC+CGG | - | chr4.4:22676760-22676779 | MS.gene32209:CDS | 55.0% | |
| GAGTCCCAATCCTCTTTCGC+CGG | + | chr4.4:22676758-22676777 | None:intergenic | 55.0% | |
| GGATGAGTTTCCGGCGAAAG+AGG | - | chr4.4:22676745-22676764 | MS.gene32209:CDS | 55.0% | |
| ! | TGATTCGGATAGCTCGTCGG+AGG | - | chr4.4:22676724-22676743 | MS.gene32209:CDS | 55.0% |
| !! | GAACGTGAGAGCACCATTGG+AGG | - | chr4.4:22676586-22676605 | MS.gene32209:CDS | 55.0% |
| AGAGGATTGGGACTCGCCGG+AGG | - | chr4.4:22676763-22676782 | MS.gene32209:CDS | 65.0% | |
| ATTGGGACTCGCCGGAGGAG+TGG | - | chr4.4:22676768-22676787 | MS.gene32209:CDS | 65.0% | |
| TTGGGACTCGCCGGAGGAGT+GGG | - | chr4.4:22676769-22676788 | MS.gene32209:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 22676170 | 22676949 | 22676170 | ID=MS.gene32209 |
| chr4.4 | mRNA | 22676170 | 22676949 | 22676170 | ID=MS.gene32209.t1;Parent=MS.gene32209 |
| chr4.4 | exon | 22676170 | 22676949 | 22676170 | ID=MS.gene32209.t1.exon1;Parent=MS.gene32209.t1 |
| chr4.4 | CDS | 22676170 | 22676949 | 22676170 | ID=cds.MS.gene32209.t1;Parent=MS.gene32209.t1 |
| Gene Sequence |
| Protein sequence |
