AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052561


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052561.t1 MTR_4g091220 92.692 260 18 1 1 259 24 283 1.72e-132 375
MS.gene052561.t1 MTR_4g091220 92.913 254 17 1 1 253 24 277 1.50e-127 363
MS.gene052561.t1 MTR_2g011760 52.330 279 92 9 1 259 1 258 1.31e-73 225
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052561.t1 AT4G21500 34.413 247 105 8 17 246 9 215 2.07e-14 70.5

Find 48 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGTTTCTCTTCTCCACATT+TGG 0.202266 4.3:+22806711 None:intergenic
AAAACAAGAAAACTGGAATA+TGG 0.269894 4.3:-22806500 MS.gene052561:CDS
TCAATTTCCTTTGGTTCAAC+TGG 0.327808 4.3:+22806676 None:intergenic
GGTGAATTCCGAAAAGAGTT+TGG 0.330170 4.3:-22806620 MS.gene052561:CDS
CTCGTCGGAGGATGAGTTTC+CGG 0.351454 4.3:-22806422 MS.gene052561:CDS
CAAGATGATGAAGAGGGATT+TGG 0.357467 4.3:-22806320 MS.gene052561:CDS
TTGATTGAGATAGCTTTAGA+TGG 0.357877 4.3:-22806346 MS.gene052561:CDS
TCTAGAGACTCAATTTCCTT+TGG 0.372592 4.3:+22806667 None:intergenic
CACTGGAGAGAAAACTATCT+TGG 0.409754 4.3:+22806861 None:intergenic
GAGCTATCCGAATCAGATTC+CGG 0.439128 4.3:+22806442 None:intergenic
AGTTTCCGGCGAAAGAGGAT+TGG 0.443586 4.3:-22806408 MS.gene052561:CDS
GGATGAGTTTCCGGCGAAAG+AGG 0.443918 4.3:-22806413 MS.gene052561:CDS
GGGAAGAAGAAGACAGAGAA+GGG 0.445414 4.3:-22806369 MS.gene052561:CDS
AAACAAGAAAACTGGAATAT+GGG 0.452373 4.3:-22806499 MS.gene052561:CDS
AAAATATCATCATGAACAAA+AGG 0.457722 4.3:-22806785 MS.gene052561:CDS
GTTTCCGGCGAAAGAGGATT+GGG 0.460419 4.3:-22806407 MS.gene052561:CDS
AGAAAAGATGAGGTAAATAG+AGG 0.464575 4.3:+22806884 None:intergenic
CTCCAGTGCTTATTCTCACC+GGG 0.467412 4.3:-22806846 MS.gene052561:CDS
TGGAAGCAAGATGATGAAGA+GGG 0.469934 4.3:-22806326 MS.gene052561:CDS
TCTCCAGTGCTTATTCTCAC+CGG 0.472662 4.3:-22806847 MS.gene052561:CDS
TTCGTTGTCACGATACTATC+CGG 0.478563 4.3:-22806461 MS.gene052561:CDS
GAGGAAAATCAAGGTGCAAA+AGG 0.482289 4.3:+22806903 None:intergenic
TTGGGACTCGCCGGAGGAGT+GGG 0.488949 4.3:-22806389 MS.gene052561:CDS
ATACTATCCGGAATCTGATT+CGG 0.492058 4.3:-22806449 MS.gene052561:CDS
ATGGAACGTGAGAGCACCAT+TGG 0.500437 4.3:-22806575 MS.gene052561:CDS
GAAAACAAAGCAACCAAATG+TGG 0.507750 4.3:-22806724 MS.gene052561:CDS
ATTGGGACTCGCCGGAGGAG+TGG 0.510971 4.3:-22806390 MS.gene052561:CDS
TCTTCGTATATTACCTCCAA+TGG 0.512351 4.3:+22806559 None:intergenic
TCTTCTTCTTCCCACTCCTC+CGG 0.516810 4.3:+22806379 None:intergenic
TGGGAAGAAGAAGACAGAGA+AGG 0.524332 4.3:-22806370 MS.gene052561:CDS
ATGGAAGCAAGATGATGAAG+AGG 0.528161 4.3:-22806327 MS.gene052561:CDS
TTGCTCACAATCCTCCGTCT+CGG 0.556266 4.3:-22806817 MS.gene052561:CDS
ATCCCGGTGAGAATAAGCAC+TGG 0.563079 4.3:+22806844 None:intergenic
ATCTGATTCGGATAGCTCGT+CGG 0.574190 4.3:-22806437 MS.gene052561:CDS
GATTGTGAGCAAGAGAATCC+CGG 0.574606 4.3:+22806828 None:intergenic
GAACGTGAGAGCACCATTGG+AGG 0.580318 4.3:-22806572 MS.gene052561:CDS
GAGTCCCAATCCTCTTTCGC+CGG 0.581065 4.3:+22806403 None:intergenic
AACATGTCCAGTTGAACCAA+AGG 0.581805 4.3:-22806683 MS.gene052561:CDS
GAAAGAGGATTGGGACTCGC+CGG 0.585561 4.3:-22806398 MS.gene052561:CDS
AGGTAAATAGAGGAAAATCA+AGG 0.599703 4.3:+22806894 None:intergenic
AGAGGATTGGGACTCGCCGG+AGG 0.612220 4.3:-22806395 MS.gene052561:CDS
TTTGATTTGCTCCGAGACGG+AGG 0.615406 4.3:+22806806 None:intergenic
TTAGTTGCACCTAATCTCAC+CGG 0.625690 4.3:+22806231 None:intergenic
ACTATCTTGGAGAAAAGATG+AGG 0.649524 4.3:+22806874 None:intergenic
GAAAACTCTTTGTACCTCGT+CGG 0.660934 4.3:+22806253 None:intergenic
TGATTCGGATAGCTCGTCGG+AGG 0.685465 4.3:-22806434 MS.gene052561:CDS
GTGACAATGCACGAAAACAG+AGG 0.690366 4.3:+22806925 None:intergenic
AGATTGATATTTCTCCGACG+AGG 0.710192 4.3:-22806267 MS.gene052561:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATATCATCATGAACAAA+AGG - chr4.3:22806434-22806453 MS.gene052561:CDS 20.0%
!! ATATGAAGATGAAGAAAAAT+TGG - chr4.3:22806683-22806702 MS.gene052561:CDS 20.0%
!!! AGATTTTGAAATAGATGAAA+TGG + chr4.3:22806256-22806275 None:intergenic 20.0%
! AAAACAAGAAAACTGGAATA+TGG - chr4.3:22806719-22806738 MS.gene052561:CDS 25.0%
! AAACAAGAAAACTGGAATAT+GGG - chr4.3:22806720-22806739 MS.gene052561:CDS 25.0%
! CAAATGAAAAACAAGAAAAC+TGG - chr4.3:22806712-22806731 MS.gene052561:CDS 25.0%
!!! CAGTTTTCTTGTTTTTCATT+TGG + chr4.3:22806714-22806733 None:intergenic 25.0%
AGAAAAGATGAGGTAAATAG+AGG + chr4.3:22806338-22806357 None:intergenic 30.0%
AGGTAAATAGAGGAAAATCA+AGG + chr4.3:22806328-22806347 None:intergenic 30.0%
! ACTTGATTCCAAACTCTTTT+CGG + chr4.3:22806610-22806629 None:intergenic 30.0%
!! TTGATTGAGATAGCTTTAGA+TGG - chr4.3:22806873-22806892 MS.gene052561:CDS 30.0%
ACTATCTTGGAGAAAAGATG+AGG + chr4.3:22806348-22806367 None:intergenic 35.0%
ATACTATCCGGAATCTGATT+CGG - chr4.3:22806770-22806789 MS.gene052561:CDS 35.0%
GAAAACAAAGCAACCAAATG+TGG - chr4.3:22806495-22806514 MS.gene052561:CDS 35.0%
TCTAGAGACTCAATTTCCTT+TGG + chr4.3:22806555-22806574 None:intergenic 35.0%
TCTTCGTATATTACCTCCAA+TGG + chr4.3:22806663-22806682 None:intergenic 35.0%
! GTTTTCTTCTTTAGCGATGA+TGG + chr4.3:22806481-22806500 None:intergenic 35.0%
! TCAATTTCCTTTGGTTCAAC+TGG + chr4.3:22806546-22806565 None:intergenic 35.0%
!!! ATTTTTGATTTGCTCCGAGA+CGG + chr4.3:22806419-22806438 None:intergenic 35.0%
AACATGTCCAGTTGAACCAA+AGG - chr4.3:22806536-22806555 MS.gene052561:CDS 40.0%
AGATTGATATTTCTCCGACG+AGG - chr4.3:22806952-22806971 MS.gene052561:CDS 40.0%
AGTGCACTGTTCAGAAAAAG+AGG - chr4.3:22806578-22806597 MS.gene052561:CDS 40.0%
ATGGAAGCAAGATGATGAAG+AGG - chr4.3:22806892-22806911 MS.gene052561:CDS 40.0%
CAAGATGATGAAGAGGGATT+TGG - chr4.3:22806899-22806918 MS.gene052561:CDS 40.0%
CACTGGAGAGAAAACTATCT+TGG + chr4.3:22806361-22806380 None:intergenic 40.0%
GAAAACTCTTTGTACCTCGT+CGG + chr4.3:22806969-22806988 None:intergenic 40.0%
GAGGAAAATCAAGGTGCAAA+AGG + chr4.3:22806319-22806338 None:intergenic 40.0%
GGTGAATTCCGAAAAGAGTT+TGG - chr4.3:22806599-22806618 MS.gene052561:CDS 40.0%
TGGAAGCAAGATGATGAAGA+GGG - chr4.3:22806893-22806912 MS.gene052561:CDS 40.0%
TTAGTTGCACCTAATCTCAC+CGG + chr4.3:22806991-22807010 None:intergenic 40.0%
TTCGTTGTCACGATACTATC+CGG - chr4.3:22806758-22806777 MS.gene052561:CDS 40.0%
! AAGAGTTTTCCGGTGAGATT+AGG - chr4.3:22806979-22806998 MS.gene052561:CDS 40.0%
! ACGAGGTACAAAGAGTTTTC+CGG - chr4.3:22806969-22806988 MS.gene052561:CDS 40.0%
! CTGTTTCTCTTCTCCACATT+TGG + chr4.3:22806511-22806530 None:intergenic 40.0%
!! CAAGTTCGAGTTTTGTGGAA+TGG - chr4.3:22806625-22806644 MS.gene052561:CDS 40.0%
!! GGAATCAAGTTCGAGTTTTG+TGG - chr4.3:22806620-22806639 MS.gene052561:CDS 40.0%
GAGCTATCCGAATCAGATTC+CGG + chr4.3:22806780-22806799 None:intergenic 45.0%
GATTGTGAGCAAGAGAATCC+CGG + chr4.3:22806394-22806413 None:intergenic 45.0%
GGGAAGAAGAAGACAGAGAA+GGG - chr4.3:22806850-22806869 MS.gene052561:CDS 45.0%
GTGACAATGCACGAAAACAG+AGG + chr4.3:22806297-22806316 None:intergenic 45.0%
TCTCCAGTGCTTATTCTCAC+CGG - chr4.3:22806372-22806391 MS.gene052561:CDS 45.0%
TGGGAAGAAGAAGACAGAGA+AGG - chr4.3:22806849-22806868 MS.gene052561:CDS 45.0%
!! ATCTGATTCGGATAGCTCGT+CGG - chr4.3:22806782-22806801 MS.gene052561:CDS 45.0%
AGTTTCCGGCGAAAGAGGAT+TGG - chr4.3:22806811-22806830 MS.gene052561:CDS 50.0%
ATCCCGGTGAGAATAAGCAC+TGG + chr4.3:22806378-22806397 None:intergenic 50.0%
CTCCAGTGCTTATTCTCACC+GGG - chr4.3:22806373-22806392 MS.gene052561:CDS 50.0%
GTTTCCGGCGAAAGAGGATT+GGG - chr4.3:22806812-22806831 MS.gene052561:CDS 50.0%
TCTTCTTCTTCCCACTCCTC+CGG + chr4.3:22806843-22806862 None:intergenic 50.0%
TTGCTCACAATCCTCCGTCT+CGG - chr4.3:22806402-22806421 MS.gene052561:CDS 50.0%
!! ATGGAACGTGAGAGCACCAT+TGG - chr4.3:22806644-22806663 MS.gene052561:CDS 50.0%
!! TTTGATTTGCTCCGAGACGG+AGG + chr4.3:22806416-22806435 None:intergenic 50.0%
CTCGTCGGAGGATGAGTTTC+CGG - chr4.3:22806797-22806816 MS.gene052561:CDS 55.0%
GAAAGAGGATTGGGACTCGC+CGG - chr4.3:22806821-22806840 MS.gene052561:CDS 55.0%
GAGTCCCAATCCTCTTTCGC+CGG + chr4.3:22806819-22806838 None:intergenic 55.0%
GGATGAGTTTCCGGCGAAAG+AGG - chr4.3:22806806-22806825 MS.gene052561:CDS 55.0%
! TGATTCGGATAGCTCGTCGG+AGG - chr4.3:22806785-22806804 MS.gene052561:CDS 55.0%
!! GAACGTGAGAGCACCATTGG+AGG - chr4.3:22806647-22806666 MS.gene052561:CDS 55.0%
AGAGGATTGGGACTCGCCGG+AGG - chr4.3:22806824-22806843 MS.gene052561:CDS 65.0%
ATTGGGACTCGCCGGAGGAG+TGG - chr4.3:22806829-22806848 MS.gene052561:CDS 65.0%
TTGGGACTCGCCGGAGGAGT+GGG - chr4.3:22806830-22806849 MS.gene052561:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 22806231 22807010 22806231 ID=MS.gene052561
chr4.3 mRNA 22806231 22807010 22806231 ID=MS.gene052561.t1;Parent=MS.gene052561
chr4.3 exon 22806231 22807010 22806231 ID=MS.gene052561.t1.exon1;Parent=MS.gene052561.t1
chr4.3 CDS 22806231 22807010 22806231 ID=cds.MS.gene052561.t1;Parent=MS.gene052561.t1
Gene Sequence

>MS.gene052561

ATGAACTCTCTATGTGTTGAAACCATTTCATCTATTTCAAAATCTTCAATGTTCTCTTCAAATCCTCTGTTTTCGTGCATTGTCACCTTTTGCACCTTGATTTTCCTCTATTTACCTCATCTTTTCTCCAAGATAGTTTTCTCTCCAGTGCTTATTCTCACCGGGATTCTCTTGCTCACAATCCTCCGTCTCGGAGCAAATCAAAAATATCATCATGAACAAAAGGATAATCAACAGAAACATGAATCCATCATCGCTAAAGAAGAAAACAAAGCAACCAAATGTGGAGAAGAGAAACAGAATTCAACATGTCCAGTTGAACCAAAGGAAATTGAGTCTCTAGAGCAAGTGCACTGTTCAGAAAAAGAGGTGAATTCCGAAAAGAGTTTGGAATCAAGTTCGAGTTTTGTGGAATGGAACGTGAGAGCACCATTGGAGGTAATATACGAAGAATATGAAGATGAAGAAAAATTGGATGATCCAAATGAAAAACAAGAAAACTGGAATATGGGTGATTCGAATTATTGTTCGTTGTCACGATACTATCCGGAATCTGATTCGGATAGCTCGTCGGAGGATGAGTTTCCGGCGAAAGAGGATTGGGACTCGCCGGAGGAGTGGGAAGAAGAAGACAGAGAAGGGTTGATTGAGATAGCTTTAGATGGAAGCAAGATGATGAAGAGGGATTTGGAGTTTCAGTATGAAGAAGAGAATTTGATTGAGATTGATATTTCTCCGACGAGGTACAAAGAGTTTTCCGGTGAGATTAGGTGCAACTAA

Protein sequence

>MS.gene052561.t1

MNSLCVETISSISKSSMFSSNPLFSCIVTFCTLIFLYLPHLFSKIVFSPVLILTGILLLTILRLGANQKYHHEQKDNQQKHESIIAKEENKATKCGEEKQNSTCPVEPKEIESLEQVHCSEKEVNSEKSLESSSSFVEWNVRAPLEVIYEEYEDEEKLDDPNEKQENWNMGDSNYCSLSRYYPESDSDSSSEDEFPAKEDWDSPEEWEEEDREGLIEIALDGSKMMKRDLEFQYEEENLIEIDISPTRYKEFSGEIRCN