AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32335

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32335.t1 MTR_1g028960 94.907 216 10 1 1 215 8 223 2.46e-148 411
MS.gene32335.t1 MTR_1g028970 91.163 215 19 0 1 215 9 223 3.51e-143 398
MS.gene32335.t1 MTR_1g028970 91.667 204 17 0 1 204 9 212 3.53e-136 380
MS.gene32335.t1 MTR_3g090530 68.056 216 68 1 1 215 7 222 2.53e-107 308
MS.gene32335.t1 MTR_1g028970 91.720 157 13 0 1 157 9 165 1.34e-102 294
MS.gene32335.t1 MTR_3g090530 68.293 205 64 1 1 204 7 211 1.31e-100 291
MS.gene32335.t1 MTR_3g090530 67.722 158 50 1 1 157 7 164 1.56e-75 226
MS.gene32335.t1 MTR_3g090530 67.550 151 48 1 66 215 7 157 6.19e-71 213
MS.gene32335.t1 MTR_3g090530 67.857 140 44 1 66 204 7 146 1.18e-64 197
MS.gene32335.t1 MTR_3g090530 66.667 93 30 1 66 157 7 99 3.62e-39 131
MS.gene32335.t1 MTR_1g075710 34.043 188 120 1 28 215 19 202 4.13e-39 134
MS.gene32335.t1 MTR_1g041495 31.383 188 125 1 28 215 19 202 3.91e-34 121
MS.gene32335.t1 MTR_1g041495 29.949 197 125 2 28 215 19 211 5.08e-31 113
MS.gene32335.t1 MTR_1g075700 35.862 145 89 1 28 172 19 159 6.80e-28 104
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32335.t1 AT1G21360 52.113 213 101 1 2 214 11 222 2.45e-75 227
MS.gene32335.t1 AT3G21260 51.835 218 100 1 2 214 15 232 4.75e-68 209
MS.gene32335.t1 AT1G21360 55.769 156 68 1 2 157 11 165 3.42e-56 177
MS.gene32335.t1 AT1G21360 51.250 160 78 0 55 214 1 160 1.03e-53 169
MS.gene32335.t1 AT3G21260 54.422 147 67 0 68 214 2 148 1.09e-45 149
MS.gene32335.t1 AT3G21260 53.846 143 66 0 72 214 1 143 4.95e-44 144
MS.gene32335.t1 AT2G33470 33.516 182 117 1 34 215 25 202 1.02e-34 122
MS.gene32335.t1 AT2G33470 33.516 182 117 1 34 215 25 202 1.02e-34 122

Find 37 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCCTCTCTATATGTTATT+TGG 0.165815 1.4:+14109508 MS.gene32335:CDS
TTTAGTTGAAATATTGAAAT+TGG 0.200840 1.4:+14115383 MS.gene32335:CDS
AAAATAATTACAGATAAAAT+AGG 0.215881 1.4:+14114327 MS.gene32335:intron
TAAGCAGAAAATGCAGATAT+TGG 0.283442 1.4:+14116118 MS.gene32335:CDS
AAAAGATCCTGAAAAGAAAA+TGG 0.298748 1.4:+14115705 MS.gene32335:CDS
GTTGTGATAGTTAAGCCTTC+TGG 0.336106 1.4:+14109449 MS.gene32335:CDS
TATTTGGTTCTACAAGTTCT+TGG 0.341649 1.4:+14109524 MS.gene32335:CDS
GGTCTTCATCTTCAGTCTTA+AGG 0.359906 1.4:-14116085 None:intergenic
ACTAAATTTAGATTCGTTGT+TGG 0.392467 1.4:-14115366 None:intergenic
GCTGCAGTAAGGCCTTTGTT+TGG 0.415497 1.4:+14115439 MS.gene32335:CDS
CACTAAAACCATGGCATGGA+TGG 0.433073 1.4:+14115758 MS.gene32335:CDS
TGTCTCAAAACAGCCATTGT+TGG 0.436087 1.4:-14114351 None:intergenic
AACAGAAGGCAATGCAAAGA+AGG 0.452284 1.4:+14115410 MS.gene32335:CDS
AAATAATTACAGATAAAATA+GGG 0.461666 1.4:+14114328 MS.gene32335:intron
TTTGCAGAGATTATATAACT+TGG 0.500380 1.4:+14116257 MS.gene32335:intron
ATAATAACGAGGTGAAATCC+AGG 0.506807 1.4:-14115669 None:intergenic
CAGTGGATATCCTCAAGAAA+AGG 0.513380 1.4:-14116155 None:intergenic
AGGCCTTTGTTTGGCTCACA+AGG 0.523024 1.4:+14115448 MS.gene32335:CDS
ATAGAGAGGAAAGGCTGAGT+GGG 0.530057 1.4:-14109497 None:intergenic
ACAGAAGGCAATGCAAAGAA+GGG 0.552195 1.4:+14115411 MS.gene32335:CDS
GAAGGGGTCTAGCTGCAGTA+AGG 0.554936 1.4:+14115428 MS.gene32335:CDS
AGATGTGTATCAAAATATCA+AGG 0.576462 1.4:+14114374 MS.gene32335:CDS
GATGAAATCCATCCATGCCA+TGG 0.583812 1.4:-14115766 None:intergenic
TTGAAATTGGAAGCAACAGA+AGG 0.584098 1.4:+14115396 MS.gene32335:CDS
ATGAAGTTACACTAAAACCA+TGG 0.602033 1.4:+14115749 MS.gene32335:CDS
GAACCAAATAACATATAGAG+AGG 0.617617 1.4:-14109511 None:intergenic
AGATAAAATAGGGCCAACAA+TGG 0.630676 1.4:+14114338 MS.gene32335:intron
AAATAACATATAGAGAGGAA+AGG 0.630937 1.4:-14109506 None:intergenic
GTTACACTAAAACCATGGCA+TGG 0.631869 1.4:+14115754 MS.gene32335:CDS
CTACATATTCTACAGGACCC+TGG 0.634914 1.4:+14115651 MS.gene32335:intron
TAATAACGAGGTGAAATCCA+GGG 0.641040 1.4:-14115668 None:intergenic
CAGAAGGCAATGCAAAGAAG+GGG 0.645808 1.4:+14115412 MS.gene32335:CDS
TCACCTTGTGAGCCAAACAA+AGG 0.647841 1.4:-14115451 None:intergenic
TAGAATTTGTAATAATAACG+AGG 0.659525 1.4:-14115680 None:intergenic
TAGAGAGGAAAGGCTGAGTG+GGG 0.664205 1.4:-14109496 None:intergenic
TATAGAGAGGAAAGGCTGAG+TGG 0.667479 1.4:-14109498 None:intergenic
TCAAGCTTACTAAGATACAG+TGG 0.685224 1.4:-14116172 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr1.4 gene 14109401 14116299 14109401 ID=MS.gene32335
chr1.4 mRNA 14109401 14116299 14109401 ID=MS.gene32335.t1;Parent=MS.gene32335
chr1.4 exon 14109401 14109545 14109401 ID=MS.gene32335.t1.exon1;Parent=MS.gene32335.t1
chr1.4 CDS 14109401 14109545 14109401 ID=cds.MS.gene32335.t1;Parent=MS.gene32335.t1
chr1.4 exon 14114340 14114395 14114340 ID=MS.gene32335.t1.exon2;Parent=MS.gene32335.t1
chr1.4 CDS 14114340 14114395 14114340 ID=cds.MS.gene32335.t1;Parent=MS.gene32335.t1
chr1.4 exon 14115339 14115469 14115339 ID=MS.gene32335.t1.exon3;Parent=MS.gene32335.t1
chr1.4 CDS 14115339 14115469 14115339 ID=cds.MS.gene32335.t1;Parent=MS.gene32335.t1
chr1.4 exon 14115666 14115801 14115666 ID=MS.gene32335.t1.exon4;Parent=MS.gene32335.t1
chr1.4 CDS 14115666 14115801 14115666 ID=cds.MS.gene32335.t1;Parent=MS.gene32335.t1
chr1.4 exon 14116041 14116184 14116041 ID=MS.gene32335.t1.exon5;Parent=MS.gene32335.t1
chr1.4 CDS 14116041 14116184 14116041 ID=cds.MS.gene32335.t1;Parent=MS.gene32335.t1
chr1.4 exon 14116264 14116299 14116264 ID=MS.gene32335.t1.exon6;Parent=MS.gene32335.t1
chr1.4 CDS 14116264 14116299 14116264 ID=cds.MS.gene32335.t1;Parent=MS.gene32335.t1
Gene Sequence

>MS.gene32335

ATGGAGAAGAAATCAGAGATAAGTTGTTTTATTGAAGAGCTTTTTATGGTTGTGATAGTTAAGCCTTCTGGAGAAAATCTTGAAGCTGCTCACATCCCCACTCAGCCTTTCCTCTCTATATGTTATTTGGTTCTACAAGTTCTTGGTATGTTTTGTTACTTCAAATCTTAATTTCATTATTATTGTTTAAATTTTGATGTGAGTAATATAGAAGGAACAATCTGTGTATATTTTAAATCATGATATATGATGTACATCAGTTGTATATATATATAAACTAATAAGCAACTGACTCCTAAATATAAGCTAAGTGATAGAGGAGCCTCTGATATGCTATTCAAAATACAGTTGCACAATTCCTTCCTATGTACAAGGAGGTGAATGATGACTATGAATCCTTATCTACTTGTTGACTAAGTACTCCTATACGCCCCCTCAAGATGGAGGTATCTTCGAGAACTCCAATCTTGTCTTTGAGAAAATGATGTCTAGTGGCAGATAGAGGTTTGGTGAGCAAATCTGCTAGTTGGTGACTTGTCGGAACATGTGAAACTTGCAGCTTCTTTTCAATAACAAGATCACGAACGAAGTGATAATCTATGGCTATATGTTTCATCCTAGAATGGAAAACTGGATTAGCACATAGATAAGTGGCGCCAATGTTGTCTGAAAATATGGTTGGAGCAGTAATTTGAATTCCGAGTTCTTGAAGCAACTGTTGAATCCATAATATTTCTGTTGTAGTAGTTGCAATTGTGCGATACTCAGCTTCCGTTGACGATCGTGCGACTGTAGGTTGCTTTTTGCATGACCAAGATATGACATTAGGCCCAAAATAAATTAAGTATGCACCAGTGGATTTCCGATCTGAAGTATCTCCAGCCCAATCTGCATCACAAAAGCCAATGAGACTTTTATTGAGGGACTTGGTGAGATGAAGTCCATGTGTTATAGTTGACTTGAGATATCGTAGAATTCTTTTTAAGGCTTGCATATGGGTCATTGTGGGGGCTTGCATGTTTTGAGCTAACTTGTTGACAGAGAAAGCTATGTCTGGTCTTGTGATAGAAAGATAGTGCAAAGATCCAAGAATGCTACGAAACTCACTACTGTCACATATTGTAGCATCAGGTGTGGATGTGGAGCAAAATGAAGTTGCCATCGGAGTTGAGACAGGCTTAGAGTCAGCCATATTTGCTCGATGAAGAATGTCTCTAATGTAGTGATGCTGAGTTAGGAATAGACCAGATTTGGTTGGAATGATCTCAACACCAAGAAAATGACTCGGCTGGCCTAAATCTTTAAGGGAGAATTGTGCTGCAAGAGACTGCTTGAACTTATCTAGGAAGCTAGAGTTGTTTCCTGTTAGTAACAAATCATCTACATAAATTAAAAAATATGCAACAATGGAACCTGATTTGTAGATGAATAATGATGTATCAGTCTTGGCATTGACAAATCCATATTTCACTAGAAACGTGCTCAAGGCATGAAACCAAGCACGAGGTGCTTGTTTGAGACCATATAATGCTTTCTTGAGCTTGCAAACATAATTAGGAAAAGATGAATGAATGAAACCAGTTGGTTGAGTCATGTAAACATCTTCATCGATGCTTCCGTGGAGGAATGCATTGTTGACATCCATTTGCGTCAAAGACCAACCATTGGAGATAGCAATGCACAGTACCAATTTAATGGTTTGTGGCTTAATCACGGGGCTGAATGTGTCCTTGTAGTCAAGACCGGGTCTTTGATGGAAACCTTTGGCTACTAATCTTGCTTTGTAGCGAGATATGGACCCATCAGGATTTCTTTTCAATCGAAACACCCATTTATTTCCAATCACATTATGATGTGGTGCAGGAGGAACTAATGTCCATGTTCCATTGCGCATGAGAGCCATGAACTCATCTGTCATTGCTTGTTGCCATTCAGGATATTTGAGAGCTTGAGACACACATGTTGGTTCAATGGGATTGGGTAATGGATATTTAGTGACTAGATGGATTTTCTTGGGTTTAAAAATTCCACTCTTTGAGCGAGTATGGACAGGATGATTAACTTTGGGTGGTTGGACTTTGGGTTTTGTTTTTGATTTATCAGGGACTATATTTGGAGGGTTGGACGAGTTATTGGGAGGTGAGGGTAGTGAGGTAAGGGTGTTGTCATTTGTGGTTTGAATTTGTTGATAATGTTGTGGGGAAACCGATGATGTTGGTTCTATTTGTTGTTGGTTCAGTTGAGATATTGGTGGGGTTGGGATTTGATTGTTTGGAGGTGGATTTGAATTTGTTAGTTGAGGTAATATAGGAGTGGACCGTATATTTGGGTTGATTGGGTGAATAGTTGTTAATGGTGAGAGATTTGAGGGATATGGATTATTTGAATTGGTATGAGAATTAGTTGTAGAATCCTGAAAGTGGGGATCCGAAAAAGGGAATGTATTCTCAACGAATTGAACATGTCTTGAGTGGTAAAGTTTCTTGGTTTTAAGACTGTAACAAATATATGCACTTTGGGTGAGACTATAACCAAGAAAAACACAAGGCTCGGAACGTGGTTGAAGTTTGTGTGTGGTATACGGTTTAAACCAAGGAAAACAAAGACATCCAAAGGATTTGAGCTTGTTATAATTGGGTTGATCGTTGAAAAGTGTGAATAATGGCGAGTTCATGTTTAAGGTTGGAGTGGGGAGACGATTGATTAGATATGCAGCTGTTTGGGCAGCATAGGACCAAAACGATGGAGGAAGGGATGCTTGATGAAGTAGGCATCTTGTTGTTTCAAGTAAATGACGGTGTTTCCTTTCACAAAGACCATTATGTTGCGGTGTGTGTGGTGGAGTTGTTAGATGTTGAATTCCATGTGTTTGAAAAAAAGTTTTGAGCTTAATGAATTCTCCACCATTATCAGAGTAGAAACTCTTGATTTTGCAATTGAACTTATTTTCCACAAGGTTTTTAAATATTGGAAAAATAATGGACACATCTGATTTGAACTTTAAAGGATACAGCCACACATACTTTGAAAAATGATCAATAAAAATAACATAGTACTTAAAACCATCGATTGAATTGAAAGGCGAAGGGCCCCAAACATCTGAATACACAATTTCTAAAGGACTAGAACTAGAAATAGAAGATTTATTGAATGGCAACTTATGGCTCTTATTGACCATGCAATGAGTACAATTATTAAAATGAACTTTGACAGCAACATTGTGAGACTTGAGAACATGATGTAGAACGCGAGATGATGGATGACCGAGCCTATTGTGCCAACATTGAGAAGATGAAGAAGACATGGTTGAATAGACATGAGGATGGGAAGATGATGGCAGTTTGTAGACATTGCCATGATTCGGACCTTGAAGAAGACTCGCCCCCGTAGTTAAATCCTTGACCTTAAAGAAAGAAGGAAAGAATTCAATGGAAACGTCATTAGTATTGCATAACTGGGACACAGAGATTAGATTTTGAGTGACTCCAGGAGAATACAAGACATTAGTAAGAGCAAGGGAACGGTAAGGTTTGGAAGAATTAAGAGTAGTAGAACCAACATGTGTAATGGGAAGAGACATACCGTTGGCAACATGAAGTTGATCATTACCACTGTAATCACTCTTCAAAGAGAGATTATTTAGATCATTGGTAATGTGGTGTGAGGCACCAGAGTCAAATAACCATTCTGGTGGTGGAATTGTTGCTTGAGCAGCATATGCAGCAGGATGAGATGATTGCTTGTGATTTCTGGTGAACTTGTTACATGTCTGAGCACCATGTCCTTTCTTAGAACACAGCTGACAGATGATGTTAGATTGTGGACGCCTTCCAGACTGATTTGCGGGAGCTGAACGAGGTTGCTGATTTCGACCTCTGTTATGATAACGAGACCGTCCACGACTTGCGTAGTTGGCCGTAACCGGAGTTTGACGATTCATCATTTCATCTCTCTGGAGATACATTTCATAGTCTATAAGCTTTATATAGAGTTCATCAAATGAGATTGGGTTGTCACGAGTTCGAATTGAAGCTGTGAATTCTCTGAATGATTGGTCCAAACCATTTAAAGCATGAATGACAAGATCCATGTCATCAACAGCATGGTCTATCAGAGATAGTTCCTCAGAGATGGATCGAATGTTCTGCAAGTATTTTGCAACAGTTTCGGACCCTTTCTTGACAGAGTTTAGGCGTTCCTTCAGTGACATAATACGCGAACGAGAACTGGTGGCAAACACACGCTCCAAGGTAAGCCACGCATCGCAGGATGTGGGTGCAGCAGACATAACTGCACGAGCTTCAGCGTCACAGGAACCGAGTAATGCGAGATAGAGGTATTTGTCCTGGCGAATCCAAAAGCTGAACTCAGGATTGTCGGAAACACCGGCTGCAGTTGCAATTTTTGCTGGAGGACGCGGTGTGGCACCAGTGACATATCCGTCAAGGTCTCGAGCAACCAGAAGAGAATGAATTTGGCGATACCAAGCGGGGTAATTGGAGGAGTTTAGTTTGATTGGGAGTTGGGTTGTGGTATTGAATTCCACAAGTTCTTTTGTAGTGCTGGAGACAGATTGTGAAAGTGAAGAGTTTGGGGATGACATAGTGGCAATTGGATCGAATTAAAGCTAGTTAAGCAAGGAGCAATCTGATACCATATAGAAGGAACAATCTGTGTATATTTTAAATCATGATATATGATGTACATCAGTTGTATATATATATATATATATAAACTAATAAGCAACTGACTCCTAAATATAAGCTAAGTGATAGAGGAGCCTCTGATATGCTATTCAAAATACAGTTGCACAATTCCTTCCTATGTACAAGGAGGTGAATGATGACTATGAATCCTTATCTACTTGTTGACTAAGTACTCCTATAAGTAACATCTCTATTAACACATTGTTTATTAAATATGGGTAAAATAATTACAGATAAAATAGGGCCAACAATGGCTGTTTTGAGACAAGATGTGTATCAAAATATCAAGGTACAAAGAAAATCCATAGCTGAAAACTTTCAATTCAGCTTATATTACTTGATTATACTAACCACCCATTTAACCTTTCTCTTATTACAATTTTGTTTTTAATAGACAGATTAAATGTTAGTGATGAATGATGTTAGTTATTAGGGCCTGAACCCTGGATTTCTTAGCTAGTACCCTTTTGTGTCCCACAGCTCAATGCTTGAGACTAAATTAATGTCAGTAATCCGAGTAGTTATCAGATTATTAGACATCATAACTACAATTGAGATTTCACTTTTCTACAATATTTAGAACTATGGTTGATCTAGTATATACCTTTTAATAATTTGAGTAGCTTGAATTCTTCGATTCAATGCGAAATAGAATGTTTTTTGGGTCGTGCTAATATGTGCCCCTAGGGCAGGGGCACATGTTAAATTTTCTATTAAAAAAAACAAGTATTTATTGAGAAAAAATTAATTTAATTTCAATCTTTTGTGAAATAAATTGACACGTTCTCTACCAAAAAAAAATTGGCACGTTTTTCGTTATAAATTTTTTTATCTTTGGTACCTTAACATGTGTCCTATCTTTAGCACATATTGCTGTGTTAGCATTTCCCATGTTTTTTTTTCTCGCACACACTCTCTCTACGTGTCCCATTGATCTAAGTGTGTAGATTCAATATTGTAAATTTAAATAACAGAATTTTAACATCAAGTTTTTCTGTTTGATAAGAAGAAATTTAACATAAAAGGTTAACTAAAGTGTTATAAGTTAGCTGGTTATAAAATTAAGGAAACCCTTCTCTTTTCATTATAATATAGGGATTGTTTTTGTGTCGGATGGGATATTTTGTATATTTCTTATATTTATATGACTTTCATTATGTACAATTTATAAAATATTGATGATTTGTCAACTACCAATGATTAGTTGTGCATTAAATTCCGTTTGATTGTAGAGATTGGAACTAATGCATGAATCAAATCCAACAACGAATCTAAATTTAGTTGAAATATTGAAATTGGAAGCAACAGAAGGCAATGCAAAGAAGGGGTCTAGCTGCAGTAAGGCCTTTGTTTGGCTCACAAGGTGATATACCTATAATAATATAATCACACCTAATAATGCTTCTCGTTTTATCATAATTTTTTATTAAAAAAAATTACTTCTCGTTTTATATAATATAAAGTAAACAATTATTGAAGAAATTTATAAATAGGTATCATCATAATTTTTATTCAAAAAAAAGGTATCATCTTAATTTATGGTGCTACATATTCTACAGGACCCTGGATTTCACCTCGTTATTATTACAAATTCTATCAAAAGATCCTGAAAAGAAAATGGAGAAAATAGTTGAAGAATCTTATGAAGTTACACTAAAACCATGGCATGGATGGATTTCATCAACTGCTGTCAGAGTAATAATCACGTTTTCGTTATTTTCTTCCATTTTTCAATCTGTATTTCATGCTATTAATTAGATGGACTTTGCATGCTATAATTTAATTTTGCAAATCAAATTTTATTCATTAATTAAATTTATAAAATATTTAGGATTCGGATCTTTACTTCAAACAAAACTTGCAACAAAGTTACGTTTTATTTTTTTACAAATTTGCACATGATAGAAAAACCCTAAATATATATTGCGTTTCAGGTGGCTCTAAGATTAGTTCCAGAAAATAAAACATTCATTGATCTCCTTAAGACTGAAGATGAAGACCGTGACATGCTTAAGCAGAAAATGCAGATATTGGTTTCTCTACTTGTGCCTTTTCTTGAGGATATCCACTGTATCTTAGTAAGCTTGATCATCAATTGGTCATGAAAATTCCTACATATTGCAAACTTTATCTTACGTGTGTGTATTTGTTTTGCAGAGATTATATAACTTGGACAAGCTGAAATCAACCTGA
Protein sequence

>MS.gene32335.t1

MEKKSEISCFIEELFMVVIVKPSGENLEAAHIPTQPFLSICYLVLQVLDKIGPTMAVLRQDVYQNIKRLELMHESNPTTNLNLVEILKLEATEGNAKKGSSCSKAFVWLTRTLDFTSLLLQILSKDPEKKMEKIVEESYEVTLKPWHGWISSTAVRVALRLVPENKTFIDLLKTEDEDRDMLKQKMQILVSLLVPFLEDIHCILRLYNLDKLKST