Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04926.t1 | XP_003600750.2 | 73.1 | 119 | 32 | 0 | 1 | 119 | 22 | 140 | 1.70E-43 | 185.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04926.t1 | P62926 | 45.9 | 111 | 46 | 6 | 6 | 114 | 27 | 125 | 2.6e-16 | 86.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04926.t1 | G7J9G6 | 73.1 | 119 | 32 | 0 | 1 | 119 | 22 | 140 | 1.2e-43 | 185.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04925 | MS.gene04926 | 0.846721 | 1.82E-59 | -1.69E-46 |
| MS.gene04926 | MS.gene050720 | 0.813935 | 1.99E-51 | -1.69E-46 |
| MS.gene04926 | MS.gene05240 | 0.802719 | 4.91E-49 | -1.69E-46 |
| MS.gene04926 | MS.gene054147 | 0.806901 | 6.57E-50 | -1.69E-46 |
| MS.gene04926 | MS.gene054277 | 0.814905 | 1.21E-51 | -1.69E-46 |
| MS.gene04926 | MS.gene054742 | 0.825783 | 3.88E-54 | -1.69E-46 |
| MS.gene04926 | MS.gene056920 | 0.808426 | 3.12E-50 | -1.69E-46 |
| MS.gene04926 | MS.gene060087 | 0.810396 | 1.18E-50 | -1.69E-46 |
| MS.gene04926 | MS.gene061038 | 0.801614 | 8.29E-49 | -1.69E-46 |
| MS.gene04926 | MS.gene066602 | 0.808782 | 2.62E-50 | -1.69E-46 |
| MS.gene04926 | MS.gene066937 | 0.808158 | 3.55E-50 | -1.69E-46 |
| MS.gene04926 | MS.gene06756 | 0.803991 | 2.68E-49 | -1.69E-46 |
| MS.gene04926 | MS.gene073870 | 0.811933 | 5.46E-51 | -1.69E-46 |
| MS.gene04926 | MS.gene08883 | 0.820013 | 8.58E-53 | -1.69E-46 |
| MS.gene04926 | MS.gene26294 | 0.82055 | 6.46E-53 | -1.69E-46 |
| MS.gene04926 | MS.gene28548 | 0.823285 | 1.50E-53 | -1.69E-46 |
| MS.gene04926 | MS.gene31835 | 0.804739 | 1.87E-49 | -1.69E-46 |
| MS.gene04926 | MS.gene31836 | 0.825322 | 4.99E-54 | -1.69E-46 |
| MS.gene04926 | MS.gene32335 | 0.830529 | 2.79E-55 | -1.69E-46 |
| MS.gene04926 | MS.gene35741 | 0.806237 | 9.07E-50 | -1.69E-46 |
| MS.gene04926 | MS.gene42005 | 0.817319 | 3.51E-52 | -1.69E-46 |
| MS.gene04926 | MS.gene44958 | 0.833292 | 5.80E-56 | -1.69E-46 |
| MS.gene04926 | MS.gene47719 | 0.810828 | 9.49E-51 | -1.69E-46 |
| MS.gene04926 | MS.gene48023 | 0.809898 | 1.51E-50 | -1.69E-46 |
| MS.gene04926 | MS.gene48807 | 0.804192 | 2.43E-49 | -1.69E-46 |
| MS.gene04926 | MS.gene48885 | 0.837568 | 4.81E-57 | -1.69E-46 |
| MS.gene04926 | MS.gene50279 | 0.825382 | 4.83E-54 | -1.69E-46 |
| MS.gene04926 | MS.gene55239 | 0.830808 | 2.38E-55 | -1.69E-46 |
| MS.gene04926 | MS.gene58786 | 0.824693 | 7.03E-54 | -1.69E-46 |
| MS.gene04926 | MS.gene63067 | 0.862901 | 3.65E-64 | -1.69E-46 |
| MS.gene04926 | MS.gene71346 | 0.807984 | 3.87E-50 | -1.69E-46 |
| MS.gene04926 | MS.gene76737 | 0.808768 | 2.63E-50 | -1.69E-46 |
| MS.gene04926 | MS.gene77033 | 0.806287 | 8.85E-50 | -1.69E-46 |
| MS.gene04926 | MS.gene77325 | 0.803402 | 3.55E-49 | -1.69E-46 |
| MS.gene04926 | MS.gene80293 | 0.801035 | 1.09E-48 | -1.69E-46 |
| MS.gene04926 | MS.gene80468 | 0.800611 | 1.33E-48 | -1.69E-46 |
| MS.gene04926 | MS.gene81504 | 0.804897 | 1.73E-49 | -1.69E-46 |
| MS.gene04926 | MS.gene81505 | 0.805309 | 1.42E-49 | -1.69E-46 |
| MS.gene04926 | MS.gene81506 | 0.802856 | 4.60E-49 | -1.69E-46 |
| MS.gene04926 | MS.gene81774 | 0.806486 | 8.04E-50 | -1.69E-46 |
| MS.gene04926 | MS.gene82245 | 0.807089 | 5.99E-50 | -1.69E-46 |
| MS.gene04926 | MS.gene84143 | 0.801324 | 9.50E-49 | -1.69E-46 |
| MS.gene04926 | MS.gene87346 | 0.820597 | 6.31E-53 | -1.69E-46 |
| MS.gene04926 | MS.gene88365 | 0.801667 | 8.08E-49 | -1.69E-46 |
| MS.gene04926 | MS.gene91223 | 0.832256 | 1.05E-55 | -1.69E-46 |
| MS.gene04926 | MS.gene91226 | 0.843611 | 1.26E-58 | -1.69E-46 |
| MS.gene04926 | MS.gene95747 | 0.803161 | 3.98E-49 | -1.69E-46 |
| MS.gene04926 | MS.gene98474 | 0.813244 | 2.82E-51 | -1.69E-46 |
| MS.gene04926 | MS.gene99062 | 0.810157 | 1.33E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04926.t1 | MTR_3g067430 | 73.109 | 119 | 32 | 0 | 1 | 119 | 22 | 140 | 4.43e-57 | 173 |
| MS.gene04926.t1 | MTR_3g067445 | 69.792 | 96 | 29 | 0 | 1 | 96 | 22 | 117 | 3.03e-42 | 135 |
| MS.gene04926.t1 | MTR_3g067540 | 55.833 | 120 | 51 | 2 | 1 | 119 | 22 | 140 | 2.65e-38 | 126 |
| MS.gene04926.t1 | MTR_3g067555 | 55.833 | 120 | 51 | 2 | 1 | 119 | 22 | 140 | 2.65e-38 | 126 |
| MS.gene04926.t1 | MTR_3g067555 | 55.462 | 119 | 53 | 0 | 1 | 119 | 9 | 127 | 3.15e-38 | 125 |
| MS.gene04926.t1 | MTR_3g067550 | 55.462 | 119 | 53 | 0 | 1 | 119 | 1 | 119 | 3.48e-38 | 125 |
| MS.gene04926.t1 | MTR_3g067555 | 55.462 | 119 | 53 | 0 | 1 | 119 | 1 | 119 | 3.48e-38 | 125 |
| MS.gene04926.t1 | MTR_3g067535 | 55.462 | 119 | 49 | 2 | 1 | 119 | 22 | 136 | 1.62e-34 | 116 |
| MS.gene04926.t1 | MTR_3g067580 | 49.593 | 123 | 50 | 3 | 1 | 119 | 30 | 144 | 1.34e-33 | 114 |
| MS.gene04926.t1 | MTR_3g067500 | 54.167 | 120 | 48 | 3 | 1 | 119 | 1 | 114 | 6.69e-31 | 106 |
| MS.gene04926.t1 | MTR_3g067280 | 54.310 | 116 | 45 | 5 | 1 | 115 | 22 | 130 | 1.12e-30 | 106 |
| MS.gene04926.t1 | MTR_6g036620 | 50.000 | 120 | 50 | 4 | 2 | 119 | 23 | 134 | 1.10e-29 | 103 |
| MS.gene04926.t1 | MTR_3g067510 | 47.899 | 119 | 52 | 2 | 1 | 119 | 27 | 135 | 2.52e-29 | 103 |
| MS.gene04926.t1 | MTR_3g067510 | 47.899 | 119 | 52 | 2 | 1 | 119 | 22 | 130 | 2.87e-29 | 102 |
| MS.gene04926.t1 | MTR_3g067570 | 53.535 | 99 | 39 | 3 | 1 | 99 | 1 | 92 | 1.29e-28 | 100 |
| MS.gene04926.t1 | MTR_3g067437 | 48.246 | 114 | 51 | 4 | 1 | 112 | 22 | 129 | 2.36e-28 | 100 |
| MS.gene04926.t1 | MTR_3g067270 | 50.862 | 116 | 49 | 4 | 1 | 115 | 22 | 130 | 4.25e-28 | 100 |
| MS.gene04926.t1 | MTR_3g467750 | 50.862 | 116 | 49 | 4 | 1 | 115 | 22 | 130 | 4.25e-28 | 100 |
| MS.gene04926.t1 | MTR_3g067830 | 68.293 | 82 | 23 | 3 | 1 | 81 | 22 | 101 | 1.79e-27 | 97.8 |
| MS.gene04926.t1 | MTR_3g467760 | 60.256 | 78 | 27 | 1 | 38 | 115 | 33 | 106 | 1.90e-26 | 95.1 |
| MS.gene04926.t1 | MTR_7g056803 | 45.082 | 122 | 59 | 3 | 1 | 119 | 22 | 138 | 4.19e-25 | 92.4 |
| MS.gene04926.t1 | MTR_3g463570 | 44.915 | 118 | 58 | 3 | 1 | 117 | 23 | 134 | 4.68e-24 | 89.7 |
| MS.gene04926.t1 | MTR_3g463570 | 44.915 | 118 | 58 | 3 | 1 | 117 | 43 | 154 | 1.32e-23 | 89.4 |
| MS.gene04926.t1 | MTR_7g056817 | 46.341 | 123 | 57 | 5 | 1 | 119 | 22 | 139 | 2.19e-23 | 88.2 |
| MS.gene04926.t1 | MTR_6g017150 | 43.333 | 120 | 55 | 7 | 1 | 115 | 22 | 133 | 6.17e-22 | 84.3 |
| MS.gene04926.t1 | MTR_3g436100 | 43.434 | 99 | 43 | 3 | 31 | 117 | 53 | 150 | 2.49e-20 | 80.9 |
| MS.gene04926.t1 | MTR_8g022430 | 41.026 | 117 | 60 | 4 | 2 | 115 | 23 | 133 | 8.06e-20 | 79.0 |
| MS.gene04926.t1 | MTR_6g017170 | 44.318 | 88 | 42 | 2 | 22 | 108 | 45 | 126 | 9.28e-20 | 78.6 |
| MS.gene04926.t1 | MTR_4g026590 | 40.323 | 124 | 58 | 4 | 1 | 115 | 23 | 139 | 1.10e-19 | 79.0 |
| MS.gene04926.t1 | MTR_6g047880 | 38.525 | 122 | 58 | 4 | 1 | 115 | 22 | 133 | 1.90e-19 | 78.2 |
| MS.gene04926.t1 | MTR_8g461020 | 40.196 | 102 | 51 | 2 | 1 | 100 | 23 | 116 | 8.42e-19 | 76.3 |
| MS.gene04926.t1 | MTR_8g022400 | 40.678 | 118 | 59 | 6 | 1 | 115 | 23 | 132 | 1.59e-18 | 75.9 |
| MS.gene04926.t1 | MTR_7g044920 | 38.806 | 134 | 59 | 5 | 1 | 117 | 21 | 148 | 2.17e-18 | 75.9 |
| MS.gene04926.t1 | MTR_8g022420 | 39.130 | 115 | 60 | 5 | 1 | 112 | 22 | 129 | 9.83e-18 | 73.9 |
| MS.gene04926.t1 | MTR_7g044980 | 40.777 | 103 | 51 | 3 | 1 | 102 | 21 | 114 | 1.54e-17 | 73.6 |
| MS.gene04926.t1 | MTR_6g047900 | 38.333 | 120 | 58 | 4 | 1 | 115 | 22 | 130 | 5.74e-17 | 71.6 |
| MS.gene04926.t1 | MTR_1g019650 | 38.679 | 106 | 47 | 3 | 1 | 105 | 22 | 110 | 7.63e-17 | 70.9 |
| MS.gene04926.t1 | MTR_8g056800 | 36.458 | 96 | 40 | 2 | 43 | 117 | 2 | 97 | 3.15e-12 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAAATTCTAATCTCAAATA+TGG | 0.216722 | 3.4:-67931626 | MS.gene04926:CDS |
| CCATATTTGAGATTAGAATT+TGG | 0.241765 | 3.4:+67931626 | None:intergenic |
| TGTACGATCGGTTATTGTTA+TGG | 0.354008 | 3.4:-67931773 | MS.gene04926:CDS |
| TTTGCTTCTACTTTCTTCAT+TGG | 0.374147 | 3.4:+67931866 | None:intergenic |
| CTTGTAAGCGTCTTGTGCTT+CGG | 0.409202 | 3.4:+67931583 | None:intergenic |
| GAAAATCGCAATGTGGTAAG+TGG | 0.449479 | 3.4:-67931743 | MS.gene04926:CDS |
| TTGACATATCAATATTAAGC+AGG | 0.480895 | 3.4:+67931512 | None:intergenic |
| TTTCTTGAATTTATAGCTAG+AGG | 0.515321 | 3.4:+67931556 | None:intergenic |
| AGGAACATCCTAAGTTATGT+TGG | 0.542665 | 3.4:-67931700 | MS.gene04926:CDS |
| TCATGAGACCAACATAACTT+AGG | 0.547157 | 3.4:+67931692 | None:intergenic |
| AAAGTAGAAGCAAATTGTGA+TGG | 0.553230 | 3.4:-67931857 | MS.gene04926:CDS |
| AAACTCGTTGGTTGTACGAT+CGG | 0.575852 | 3.4:-67931785 | MS.gene04926:CDS |
| AGCACAAGACGCTTACAAGA+TGG | 0.577959 | 3.4:-67931579 | MS.gene04926:CDS |
| AAAATCGCAATGTGGTAAGT+GGG | 0.583821 | 3.4:-67931742 | MS.gene04926:CDS |
| GGTTGTAAATGCAAACTCGT+TGG | 0.593162 | 3.4:-67931797 | MS.gene04926:CDS |
| GTCTTGTGCTTCGGAGACAG+AGG | 0.642879 | 3.4:+67931592 | None:intergenic |
| TTTATGCTAGACTGTGATAG+CGG | 0.659883 | 3.4:-67931818 | MS.gene04926:CDS |
| TACCTGCGAAAATCGCAATG+TGG | 0.731640 | 3.4:-67931750 | MS.gene04926:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CCAAATTCTAATCTCAAATA+TGG | - | chr3.4:67931763-67931782 | MS.gene04926:CDS | 25.0% |
| ! | TTTCTTGAATTTATAGCTAG+AGG | + | chr3.4:67931836-67931855 | None:intergenic | 25.0% |
| !! | CCATATTTGAGATTAGAATT+TGG | + | chr3.4:67931766-67931785 | None:intergenic | 25.0% |
| AAAGTAGAAGCAAATTGTGA+TGG | - | chr3.4:67931532-67931551 | MS.gene04926:CDS | 30.0% | |
| CATGATGAATGCACAAAAAA+GGG | - | chr3.4:67931715-67931734 | MS.gene04926:CDS | 30.0% | |
| TCATGATGAATGCACAAAAA+AGG | - | chr3.4:67931714-67931733 | MS.gene04926:CDS | 30.0% | |
| AAAATCGCAATGTGGTAAGT+GGG | - | chr3.4:67931647-67931666 | MS.gene04926:CDS | 35.0% | |
| AATGCACAAAAAAGGGTAGT+GGG | - | chr3.4:67931722-67931741 | MS.gene04926:CDS | 35.0% | |
| AGGAACATCCTAAGTTATGT+TGG | - | chr3.4:67931689-67931708 | MS.gene04926:CDS | 35.0% | |
| TCATGAGACCAACATAACTT+AGG | + | chr3.4:67931700-67931719 | None:intergenic | 35.0% | |
| TGTACGATCGGTTATTGTTA+TGG | - | chr3.4:67931616-67931635 | MS.gene04926:CDS | 35.0% | |
| TTTATGCTAGACTGTGATAG+CGG | - | chr3.4:67931571-67931590 | MS.gene04926:CDS | 35.0% | |
| ! | GAATTTTCAGAGGAAGGTTA+AGG | - | chr3.4:67931669-67931688 | MS.gene04926:CDS | 35.0% |
| GAAAATCGCAATGTGGTAAG+TGG | - | chr3.4:67931646-67931665 | MS.gene04926:CDS | 40.0% | |
| GAATGCACAAAAAAGGGTAG+TGG | - | chr3.4:67931721-67931740 | MS.gene04926:CDS | 40.0% | |
| GGTTGTAAATGCAAACTCGT+TGG | - | chr3.4:67931592-67931611 | MS.gene04926:CDS | 40.0% | |
| ! | AAACTCGTTGGTTGTACGAT+CGG | - | chr3.4:67931604-67931623 | MS.gene04926:CDS | 40.0% |
| ! | AAGTGGGAATTTTCAGAGGA+AGG | - | chr3.4:67931663-67931682 | MS.gene04926:CDS | 40.0% |
| ! | TGGTAAGTGGGAATTTTCAG+AGG | - | chr3.4:67931659-67931678 | MS.gene04926:CDS | 40.0% |
| AGCACAAGACGCTTACAAGA+TGG | - | chr3.4:67931810-67931829 | MS.gene04926:CDS | 45.0% | |
| CTTGTAAGCGTCTTGTGCTT+CGG | + | chr3.4:67931809-67931828 | None:intergenic | 45.0% | |
| TACCTGCGAAAATCGCAATG+TGG | - | chr3.4:67931639-67931658 | MS.gene04926:CDS | 45.0% | |
| ! | TACCACATTGCGATTTTCGC+AGG | + | chr3.4:67931644-67931663 | None:intergenic | 45.0% |
| GTCTTGTGCTTCGGAGACAG+AGG | + | chr3.4:67931800-67931819 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 67931526 | 67931885 | 67931526 | ID=MS.gene04926 |
| chr3.4 | mRNA | 67931526 | 67931885 | 67931526 | ID=MS.gene04926.t1;Parent=MS.gene04926 |
| chr3.4 | exon | 67931526 | 67931885 | 67931526 | ID=MS.gene04926.t1.exon1;Parent=MS.gene04926.t1 |
| chr3.4 | CDS | 67931526 | 67931885 | 67931526 | ID=cds.MS.gene04926.t1;Parent=MS.gene04926.t1 |
| Gene Sequence |
| Protein sequence |
