Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32561.t1 | XP_003616408.2 | 95.6 | 135 | 5 | 1 | 1 | 134 | 25 | 159 | 1.90E-67 | 265 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32561.t1 | A2WWV5 | 43.3 | 127 | 72 | 0 | 3 | 129 | 30 | 156 | 5.6e-28 | 125.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32561.t1 | G7KBR3 | 95.6 | 135 | 5 | 1 | 1 | 134 | 25 | 159 | 1.4e-67 | 265.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050780 | MS.gene32561 | 0.824006 | 1.02E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32561.t1 | MTR_5g079890 | 95.556 | 135 | 5 | 1 | 1 | 134 | 25 | 159 | 4.07e-94 | 268 |
| MS.gene32561.t1 | MTR_5g079890 | 96.522 | 115 | 3 | 1 | 1 | 114 | 25 | 139 | 1.52e-79 | 231 |
| MS.gene32561.t1 | MTR_5g079880 | 59.690 | 129 | 51 | 1 | 3 | 131 | 26 | 153 | 3.48e-50 | 157 |
| MS.gene32561.t1 | MTR_8g032320 | 44.275 | 131 | 59 | 4 | 3 | 130 | 26 | 145 | 7.59e-31 | 108 |
| MS.gene32561.t1 | MTR_8g032300 | 39.695 | 131 | 65 | 4 | 3 | 130 | 26 | 145 | 9.52e-27 | 97.8 |
| MS.gene32561.t1 | MTR_1g008950 | 39.640 | 111 | 56 | 3 | 3 | 113 | 26 | 125 | 1.21e-23 | 89.4 |
| MS.gene32561.t1 | MTR_8g030870 | 30.088 | 113 | 78 | 1 | 5 | 116 | 28 | 140 | 1.54e-14 | 67.0 |
| MS.gene32561.t1 | MTR_7g075960 | 42.857 | 56 | 28 | 1 | 3 | 58 | 2 | 53 | 4.39e-11 | 55.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32561.t1 | AT3G55470 | 44.094 | 127 | 71 | 0 | 3 | 129 | 26 | 152 | 2.26e-35 | 119 |
| MS.gene32561.t1 | AT3G55470 | 43.750 | 128 | 71 | 1 | 3 | 129 | 26 | 153 | 1.62e-33 | 115 |
| MS.gene32561.t1 | AT1G63220 | 38.346 | 133 | 67 | 5 | 3 | 131 | 26 | 147 | 9.22e-22 | 85.1 |
| MS.gene32561.t1 | AT1G63220 | 36.765 | 136 | 65 | 6 | 6 | 131 | 9 | 133 | 2.45e-18 | 75.9 |
| MS.gene32561.t1 | AT4G00467 | 30.189 | 106 | 74 | 0 | 5 | 110 | 28 | 133 | 9.56e-16 | 70.5 |
| MS.gene32561.t1 | AT4G00467 | 30.189 | 106 | 74 | 0 | 5 | 110 | 28 | 133 | 1.33e-15 | 70.1 |
Find 23 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATCTATTGTAAAGATTTAT+TGG | 0.151708 | 5.1:+62061449 | MS.gene32561:CDS |
| TTGGCTCAAGGTGCTCAAAA+TGG | 0.318840 | 5.1:+62061468 | MS.gene32561:CDS |
| CAAAGTCATTCTCAAAATAA+TGG | 0.344213 | 5.1:+62061274 | MS.gene32561:CDS |
| TTGAAGAAGACGTAGGAGGA+TGG | 0.471569 | 5.1:+62061755 | MS.gene32561:CDS |
| CAATCTCTCCACGATAAGAT+TGG | 0.475600 | 5.1:-62061536 | None:intergenic |
| GTAGAGGCAGAAATCATGTA+TGG | 0.482652 | 5.1:+62061201 | MS.gene32561:CDS |
| CATAGGAGCCGTGTGGCTAA+AGG | 0.495986 | 5.1:+62061179 | MS.gene32561:CDS |
| TTGGTATTGTATCAAAACAT+AGG | 0.499452 | 5.1:-62061147 | None:intergenic |
| AATTCATTCACAGGTGGAAG+AGG | 0.508492 | 5.1:+62061726 | MS.gene32561:intron |
| TGTAAAGATTTATTGGCTCA+AGG | 0.515900 | 5.1:+62061456 | MS.gene32561:CDS |
| TGCCTCTACCTTTAGCCACA+CGG | 0.519437 | 5.1:-62061187 | None:intergenic |
| AGCCGTGTGGCTAAAGGTAG+AGG | 0.540209 | 5.1:+62061185 | MS.gene32561:CDS |
| GGCTCCTATGCTCTTGACCT+TGG | 0.540922 | 5.1:-62061166 | None:intergenic |
| TGGTATTGTATCAAAACATA+GGG | 0.548025 | 5.1:-62061146 | None:intergenic |
| GATTTAATTGAAGAAGACGT+AGG | 0.553025 | 5.1:+62061748 | MS.gene32561:CDS |
| AATACCAAGGTCAAGAGCAT+AGG | 0.590229 | 5.1:+62061162 | MS.gene32561:CDS |
| CGAACAACTCTATACTTGAG+AGG | 0.601631 | 5.1:-62061507 | None:intergenic |
| TTAATTGAAGAAGACGTAGG+AGG | 0.608884 | 5.1:+62061751 | MS.gene32561:CDS |
| TAGAGGCAGAAATCATGTAT+GGG | 0.615533 | 5.1:+62061202 | MS.gene32561:CDS |
| ATGTTCAAAGTAGAAAACAA+TGG | 0.628110 | 5.1:+62061236 | MS.gene32561:CDS |
| CGTGCAGACCAATCTTATCG+TGG | 0.690565 | 5.1:+62061528 | MS.gene32561:CDS |
| ACAACTCTATACTTGAGAGG+TGG | 0.693150 | 5.1:-62061504 | None:intergenic |
| TCAAGAGCATAGGAGCCGTG+TGG | 0.747555 | 5.1:+62061172 | MS.gene32561:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATATTAATTAATTACCTT+TGG | - | chr5.1:62061582-62061601 | None:intergenic | 10.0% |
| !! | TCAAAAAAAATATATAGTTA+TGG | + | chr5.1:62061656-62061675 | MS.gene32561:intron | 10.0% |
| !!! | AACTATATATTTTTTTTGAA+GGG | - | chr5.1:62061655-62061674 | None:intergenic | 10.0% |
| !!! | TAACTATATATTTTTTTTGA+AGG | - | chr5.1:62061656-62061675 | None:intergenic | 10.0% |
| !!! | TATTTCATTTTTGTTAATTA+AGG | + | chr5.1:62061605-62061624 | MS.gene32561:intron | 10.0% |
| !! | AATCTATTGTAAAGATTTAT+TGG | + | chr5.1:62061449-62061468 | MS.gene32561:CDS | 15.0% |
| !! | AAAAATGAGACATTATTGTT+AGG | - | chr5.1:62061372-62061391 | None:intergenic | 20.0% |
| !! | CATGTAACAATATATAGTTA+TGG | + | chr5.1:62061628-62061647 | MS.gene32561:intron | 20.0% |
| !! | TTTACAATAGATTCTGTTTA+AGG | - | chr5.1:62061442-62061461 | None:intergenic | 20.0% |
| !!! | AGTTGATTAATTTTCTTTGT+TGG | + | chr5.1:62061703-62061722 | MS.gene32561:intron | 20.0% |
| ! | ATGTTCAAAGTAGAAAACAA+TGG | + | chr5.1:62061236-62061255 | MS.gene32561:CDS | 25.0% |
| ! | CAAAGTCATTCTCAAAATAA+TGG | + | chr5.1:62061274-62061293 | MS.gene32561:CDS | 25.0% |
| ! | TGGTATTGTATCAAAACATA+GGG | - | chr5.1:62061149-62061168 | None:intergenic | 25.0% |
| ! | TTGGTATTGTATCAAAACAT+AGG | - | chr5.1:62061150-62061169 | None:intergenic | 25.0% |
| !! | TATTGTTAGGAGTATATATG+TGG | - | chr5.1:62061359-62061378 | None:intergenic | 25.0% |
| !!! | TATGTTTTGATACAATACCA+AGG | + | chr5.1:62061149-62061168 | MS.gene32561:CDS | 25.0% |
| GATTTAATTGAAGAAGACGT+AGG | + | chr5.1:62061748-62061767 | MS.gene32561:CDS | 30.0% | |
| !! | TGCTATTACTTTTACTCCAA+AGG | + | chr5.1:62061563-62061582 | MS.gene32561:CDS | 30.0% |
| !! | TGTAAAGATTTATTGGCTCA+AGG | + | chr5.1:62061456-62061475 | MS.gene32561:CDS | 30.0% |
| CTTTGTTGGAATTCATTCAC+AGG | + | chr5.1:62061717-62061736 | MS.gene32561:intron | 35.0% | |
| TAGAGGCAGAAATCATGTAT+GGG | + | chr5.1:62061202-62061221 | MS.gene32561:CDS | 35.0% | |
| TTAATTGAAGAAGACGTAGG+AGG | + | chr5.1:62061751-62061770 | MS.gene32561:CDS | 35.0% | |
| ! | GTTAGGAGTATATATGTGGA+AGG | - | chr5.1:62061355-62061374 | None:intergenic | 35.0% |
| ! | TTTTCTGCTGATGACTTCAT+TGG | + | chr5.1:62061308-62061327 | MS.gene32561:CDS | 35.0% |
| AATTCATTCACAGGTGGAAG+AGG | + | chr5.1:62061726-62061745 | MS.gene32561:intron | 40.0% | |
| ACAACTCTATACTTGAGAGG+TGG | - | chr5.1:62061507-62061526 | None:intergenic | 40.0% | |
| CAATCTCTCCACGATAAGAT+TGG | - | chr5.1:62061539-62061558 | None:intergenic | 40.0% | |
| CGAACAACTCTATACTTGAG+AGG | - | chr5.1:62061510-62061529 | None:intergenic | 40.0% | |
| GTAGAGGCAGAAATCATGTA+TGG | + | chr5.1:62061201-62061220 | MS.gene32561:CDS | 40.0% | |
| TGTTGGAATTCATTCACAGG+TGG | + | chr5.1:62061720-62061739 | MS.gene32561:intron | 40.0% | |
| ! | AATACCAAGGTCAAGAGCAT+AGG | + | chr5.1:62061162-62061181 | MS.gene32561:CDS | 40.0% |
| TTGAAGAAGACGTAGGAGGA+TGG | + | chr5.1:62061755-62061774 | MS.gene32561:CDS | 45.0% | |
| ! | TTGGCTCAAGGTGCTCAAAA+TGG | + | chr5.1:62061468-62061487 | MS.gene32561:CDS | 45.0% |
| !! | TGGAAGGCATTAGTCTTACG+TGG | - | chr5.1:62061339-62061358 | None:intergenic | 45.0% |
| CGTGCAGACCAATCTTATCG+TGG | + | chr5.1:62061528-62061547 | MS.gene32561:CDS | 50.0% | |
| TGCCTCTACCTTTAGCCACA+CGG | - | chr5.1:62061190-62061209 | None:intergenic | 50.0% | |
| AGCCGTGTGGCTAAAGGTAG+AGG | + | chr5.1:62061185-62061204 | MS.gene32561:CDS | 55.0% | |
| CATAGGAGCCGTGTGGCTAA+AGG | + | chr5.1:62061179-62061198 | MS.gene32561:CDS | 55.0% | |
| GGCTCCTATGCTCTTGACCT+TGG | - | chr5.1:62061169-62061188 | None:intergenic | 55.0% | |
| ! | TCAAGAGCATAGGAGCCGTG+TGG | + | chr5.1:62061172-62061191 | MS.gene32561:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 62061137 | 62061804 | 62061137 | ID=MS.gene32561 |
| chr5.1 | mRNA | 62061137 | 62061804 | 62061137 | ID=MS.gene32561.t1;Parent=MS.gene32561 |
| chr5.1 | exon | 62061137 | 62061339 | 62061137 | ID=MS.gene32561.t1.exon1;Parent=MS.gene32561.t1 |
| chr5.1 | CDS | 62061137 | 62061339 | 62061137 | ID=cds.MS.gene32561.t1;Parent=MS.gene32561.t1 |
| chr5.1 | exon | 62061449 | 62061584 | 62061449 | ID=MS.gene32561.t1.exon2;Parent=MS.gene32561.t1 |
| chr5.1 | CDS | 62061449 | 62061584 | 62061449 | ID=cds.MS.gene32561.t1;Parent=MS.gene32561.t1 |
| chr5.1 | exon | 62061739 | 62061804 | 62061739 | ID=MS.gene32561.t1.exon3;Parent=MS.gene32561.t1 |
| chr5.1 | CDS | 62061739 | 62061804 | 62061739 | ID=cds.MS.gene32561.t1;Parent=MS.gene32561.t1 |
| Gene Sequence |
| Protein sequence |
