AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32575

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 81 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTTCGTCGGTGGGGTTCT+TGG 0.156808 8.4:-48477829 None:intergenic
GAGTTGCTGGTTCTGGTTAC+TGG 0.209450 8.4:+48477156 MS.gene32575:CDS
GGTGCAGGCAAGGCGAATTT+TGG 0.246467 8.4:+48477068 MS.gene32575:intron
TTTGATCCTTGGGTTCTTCC+AGG 0.310994 8.4:+48476741 MS.gene32575:CDS
CTTGTACAAGTTTGATCCTT+GGG 0.318790 8.4:+48476731 MS.gene32575:CDS
ATTGAATCCTCACCGGAGTT+TGG 0.319990 8.4:+48477708 MS.gene32575:CDS
CCAAACAGAGTTGCTGGTTC+TGG 0.323652 8.4:+48477149 MS.gene32575:CDS
AAGTCAATTTCAGCAATAAT+TGG 0.338246 8.4:-48476711 None:intergenic
TTGTTAAGTTTCATCATAAT+GGG 0.344250 8.4:+48476564 None:intergenic
TTTGTTAAGTTTCATCATAA+TGG 0.350596 8.4:+48476563 None:intergenic
CGAAATGTTGGACTCGTTCC+TGG 0.353931 8.4:+48477560 MS.gene32575:CDS
CTGTTTGGTCTAGAACCATT+TGG 0.354289 8.4:-48477134 None:intergenic
CATTCTTTGCTTGAAAATGT+TGG 0.363451 8.4:-48477498 None:intergenic
AGCTCTGATCATCAATTTCC+AGG 0.378659 8.4:-48477578 None:intergenic
CTTTGAACCTCTTCGTCGGT+GGG 0.389977 8.4:-48477837 None:intergenic
TATCTATGTCGTAAAGTTGC+AGG 0.400628 8.4:+48476672 MS.gene32575:CDS
TTTGGATATTTCCTGTCCCT+TGG 0.404470 8.4:-48477116 None:intergenic
TCTCCTCTTGCTGTAGCATT+CGG 0.405277 8.4:-48477626 None:intergenic
TCCTCACCGGAGTTTGGATT+AGG 0.414541 8.4:+48477714 MS.gene32575:CDS
CGTGCCACCGTTTGGTAACA+TGG 0.418390 8.4:+48477791 MS.gene32575:CDS
CCAGAACCAGCAACTCTGTT+TGG 0.423168 8.4:-48477149 None:intergenic
TCTAGACCAAACAGAGTTGC+TGG 0.423965 8.4:+48477143 MS.gene32575:CDS
ATTGATGCAGCTTGATGATT+GGG 0.428244 8.4:+48477428 MS.gene32575:intron
TATTGATGCAGCTTGATGAT+TGG 0.443903 8.4:+48477427 MS.gene32575:intron
TGGATTAGGAAACCAGAGTC+AGG 0.444473 8.4:+48477728 MS.gene32575:CDS
ACTTGTACAAGTTTGATCCT+TGG 0.451700 8.4:+48476730 MS.gene32575:CDS
CTACAGCAAGAGGAGAAGTT+CGG 0.456841 8.4:+48477633 MS.gene32575:CDS
TACAGCAAGAGGAGAAGTTC+GGG 0.459516 8.4:+48477634 MS.gene32575:CDS
GTTCCTTCCGTGCCACCGTT+TGG 0.466910 8.4:+48477783 MS.gene32575:CDS
GGGTTTCACAACATGGGTGC+TGG 0.473359 8.4:+48477654 MS.gene32575:CDS
ACAACTGAAGGTAGAAAAGT+TGG 0.474990 8.4:+48477206 MS.gene32575:CDS
AACAAGAAGCTCCTCATCAG+TGG 0.476509 8.4:-48476646 None:intergenic
TTCGCCTTCATCGTCGTCCC+AGG 0.477914 8.4:+48477533 MS.gene32575:CDS
GCTTTGAACCTCTTCGTCGG+TGG 0.485096 8.4:-48477838 None:intergenic
TGTGAAAGAGGATCTCTCTC+TGG 0.490458 8.4:-48476591 None:intergenic
GTTTGGTAACATGGACTTGT+CGG 0.494850 8.4:+48477800 MS.gene32575:CDS
AGTCAGGGGATGCTGAATTA+CGG 0.495349 8.4:+48477744 MS.gene32575:CDS
GCTTCGCGAAAACACAATCT+TGG 0.501596 8.4:+48477317 MS.gene32575:CDS
GAAGTTCGGGTTTCACAACA+TGG 0.506769 8.4:+48477647 MS.gene32575:CDS
TATATTGGCAAAGCACCAAA+AGG 0.512258 8.4:+48477251 MS.gene32575:CDS
GGTAAACTCAACTGTGAAAG+AGG 0.512342 8.4:-48476603 None:intergenic
ATTTCTTCAGTCCAAGGGAC+AGG 0.517559 8.4:+48477105 MS.gene32575:CDS
AATTCAGCATCCCCTGACTC+TGG 0.524401 8.4:-48477740 None:intergenic
GACAAAATTATCACAACTGA+AGG 0.526909 8.4:+48477194 MS.gene32575:CDS
AGTCCAACATTTCGTCCACC+TGG 0.531736 8.4:-48477551 None:intergenic
TCAAGCAAAGAATGCAGCAA+TGG 0.536044 8.4:+48477507 MS.gene32575:CDS
CAGATTCTTCCCCACTGATG+AGG 0.543031 8.4:+48476635 MS.gene32575:CDS
GTGAGGATTCAATATCTGTC+GGG 0.549879 8.4:-48477698 None:intergenic
ATCAGAGCTTTGCATTGCCG+CGG 0.552759 8.4:+48477592 MS.gene32575:CDS
GTCCCAGGTGGACGAAATGT+TGG 0.553604 8.4:+48477548 MS.gene32575:CDS
AATGGTATTTCTTCAGTCCA+AGG 0.554883 8.4:+48477099 MS.gene32575:CDS
ACAAGAAGCTCCTCATCAGT+GGG 0.557935 8.4:-48476645 None:intergenic
GCTGCTTTGAACCTCTTCGT+CGG 0.558434 8.4:-48477841 None:intergenic
TCCACCTGGGACGACGATGA+AGG 0.560826 8.4:-48477537 None:intergenic
GGTGAGGATTCAATATCTGT+CGG 0.563156 8.4:-48477699 None:intergenic
ACTCCGAATGCTACAGCAAG+AGG 0.568493 8.4:+48477623 MS.gene32575:CDS
TCAGAGCTTTGCATTGCCGC+GGG 0.574657 8.4:+48477593 MS.gene32575:CDS
GACAAGTCCATGTTACCAAA+CGG 0.575738 8.4:-48477798 None:intergenic
TTTGAACCTCTTCGTCGGTG+GGG 0.576389 8.4:-48477836 None:intergenic
TTACCAAACGGTGGCACGGA+AGG 0.576728 8.4:-48477786 None:intergenic
GGATTAGGAAACCAGAGTCA+GGG 0.583546 8.4:+48477729 MS.gene32575:CDS
CAAGAACCCCACCGACGAAG+AGG 0.586414 8.4:+48477830 MS.gene32575:CDS
ATGGTATTTCTTCAGTCCAA+GGG 0.588129 8.4:+48477100 MS.gene32575:CDS
AGGGACAGGAAATATCCAAA+TGG 0.592563 8.4:+48477119 MS.gene32575:CDS
TCTGGTTACTGGAAAGCCAC+AGG 0.607999 8.4:+48477167 MS.gene32575:CDS
GCCTTCATCGTCGTCCCAGG+TGG 0.609301 8.4:+48477536 MS.gene32575:CDS
CATGTTACCAAACGGTGGCA+CGG 0.615690 8.4:-48477790 None:intergenic
GTCCAACATTTCGTCCACCT+GGG 0.623948 8.4:-48477550 None:intergenic
TAATTACCTGGAAGAACCCA+AGG 0.624355 8.4:-48476747 None:intergenic
GACAGATATTGAATCCTCAC+CGG 0.626126 8.4:+48477701 MS.gene32575:CDS
GATTAGGAAACCAGAGTCAG+GGG 0.641898 8.4:+48477730 MS.gene32575:CDS
TGGTAACATGGACTTGTCGG+CGG 0.643282 8.4:+48477803 MS.gene32575:CDS
GTGGGGAAGAATCTGAAACC+AGG 0.652605 8.4:-48476627 None:intergenic
TCACAGTTGAGTTTACCACC+TGG 0.654718 8.4:+48476609 MS.gene32575:CDS
TGGTTTCCTAATCCAAACTC+CGG 0.659176 8.4:-48477720 None:intergenic
TCCTAATCCAAACTCCGGTG+AGG 0.662436 8.4:-48477715 None:intergenic
AAGTTCGGGTTTCACAACAT+GGG 0.678618 8.4:+48477648 MS.gene32575:CDS
ATTCGGAGTGATGTTACCCG+CGG 0.710388 8.4:-48477609 None:intergenic
AAGTCCATGTTACCAAACGG+TGG 0.730264 8.4:-48477795 None:intergenic
GGGAAGAATCTGAAACCAGG+TGG 0.741808 8.4:-48476624 None:intergenic
CAAGAAGCTCCTCATCAGTG+GGG 0.747868 8.4:-48476644 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CAATTTTAACATTGTTGTTA+TGG + chr8.4:48477041-48477060 MS.gene32575:intron 20.0%
! AAGTCAATTTCAGCAATAAT+TGG - chr8.4:48476714-48476733 None:intergenic 25.0%
! CAAAAGGAACAAAAACTAAT+TGG + chr8.4:48477267-48477286 MS.gene32575:CDS 25.0%
! CTGAAATTCTGACAATAATA+CGG + chr8.4:48476928-48476947 MS.gene32575:intron 25.0%
!!! AAAGCACTTGTTTTCTATAT+TGG + chr8.4:48477236-48477255 MS.gene32575:CDS 25.0%
!!! CAATTAGTTTTTGTTCCTTT+TGG - chr8.4:48477269-48477288 None:intergenic 25.0%
!!! TAACATTGTTGTTATGGTTT+TGG + chr8.4:48477047-48477066 MS.gene32575:intron 25.0%
CAGGTAATTATCACCAATTT+AGG + chr8.4:48476760-48476779 MS.gene32575:intron 30.0%
CATTCTTTGCTTGAAAATGT+TGG - chr8.4:48477501-48477520 None:intergenic 30.0%
CTCATCAATAAAAATGAGCA+AGG - chr8.4:48477379-48477398 None:intergenic 30.0%
GACAAAATTATCACAACTGA+AGG + chr8.4:48477194-48477213 MS.gene32575:CDS 30.0%
TAACTATTGCAAAATCACAG+TGG - chr8.4:48476875-48476894 None:intergenic 30.0%
! CTAAATTGGTGATAATTACC+TGG - chr8.4:48476762-48476781 None:intergenic 30.0%
AATGGTATTTCTTCAGTCCA+AGG + chr8.4:48477099-48477118 MS.gene32575:CDS 35.0%
ACAACTGAAGGTAGAAAAGT+TGG + chr8.4:48477206-48477225 MS.gene32575:CDS 35.0%
ACTTGTACAAGTTTGATCCT+TGG + chr8.4:48476730-48476749 MS.gene32575:CDS 35.0%
ATGGTATTTCTTCAGTCCAA+GGG + chr8.4:48477100-48477119 MS.gene32575:CDS 35.0%
CTTGTACAAGTTTGATCCTT+GGG + chr8.4:48476731-48476750 MS.gene32575:CDS 35.0%
TATCTATGTCGTAAAGTTGC+AGG + chr8.4:48476672-48476691 MS.gene32575:CDS 35.0%
TCTGCAATTGCAACCTAAAT+TGG - chr8.4:48476776-48476795 None:intergenic 35.0%
! ATTGATGCAGCTTGATGATT+GGG + chr8.4:48477428-48477447 MS.gene32575:intron 35.0%
! GATAATTTTGTCTGTTCCTG+TGG - chr8.4:48477186-48477205 None:intergenic 35.0%
! TATATTGGCAAAGCACCAAA+AGG + chr8.4:48477251-48477270 MS.gene32575:CDS 35.0%
! TATTGATGCAGCTTGATGAT+TGG + chr8.4:48477427-48477446 MS.gene32575:intron 35.0%
!! CGAATTTTGGTGAGAAAGAA+TGG + chr8.4:48477081-48477100 MS.gene32575:CDS 35.0%
AGGGACAGGAAATATCCAAA+TGG + chr8.4:48477119-48477138 MS.gene32575:CDS 40.0%
CCGTAAGTTTGTCAGAATCA+CGG + chr8.4:48476845-48476864 MS.gene32575:intron 40.0%
CCGTGATTCTGACAAACTTA+CGG - chr8.4:48476848-48476867 None:intergenic 40.0%
GAAAAACTCAAGCTCGCAAA+AGG + chr8.4:48477470-48477489 MS.gene32575:CDS 40.0%
GACAAGTCCATGTTACCAAA+CGG - chr8.4:48477801-48477820 None:intergenic 40.0%
GACAGATATTGAATCCTCAC+CGG + chr8.4:48477701-48477720 MS.gene32575:CDS 40.0%
GGTAAACTCAACTGTGAAAG+AGG - chr8.4:48476606-48476625 None:intergenic 40.0%
GGTGAGGATTCAATATCTGT+CGG - chr8.4:48477702-48477721 None:intergenic 40.0%
GTGAGGATTCAATATCTGTC+GGG - chr8.4:48477701-48477720 None:intergenic 40.0%
TAATTACCTGGAAGAACCCA+AGG - chr8.4:48476750-48476769 None:intergenic 40.0%
TCAAGCAAAGAATGCAGCAA+TGG + chr8.4:48477507-48477526 MS.gene32575:CDS 40.0%
TGGTTTCCTAATCCAAACTC+CGG - chr8.4:48477723-48477742 None:intergenic 40.0%
TTTGGATATTTCCTGTCCCT+TGG - chr8.4:48477119-48477138 None:intergenic 40.0%
! AAGTTCGGGTTTCACAACAT+GGG + chr8.4:48477648-48477667 MS.gene32575:CDS 40.0%
! AGCTCTGATCATCAATTTCC+AGG - chr8.4:48477581-48477600 None:intergenic 40.0%
! CTGTTTGGTCTAGAACCATT+TGG - chr8.4:48477137-48477156 None:intergenic 40.0%
! GTTTGGTAACATGGACTTGT+CGG + chr8.4:48477800-48477819 MS.gene32575:CDS 40.0%
! TGCTGGACTTTTCTCTGATT+GGG + chr8.4:48477671-48477690 MS.gene32575:CDS 40.0%
!!! TGTTGTTATGGTTTTGGTGC+AGG + chr8.4:48477053-48477072 MS.gene32575:intron 40.0%
AACAAGAAGCTCCTCATCAG+TGG - chr8.4:48476649-48476668 None:intergenic 45.0%
AAGTCCATGTTACCAAACGG+TGG - chr8.4:48477798-48477817 None:intergenic 45.0%
ACAAGAAGCTCCTCATCAGT+GGG - chr8.4:48476648-48476667 None:intergenic 45.0%
AGTCAGGGGATGCTGAATTA+CGG + chr8.4:48477744-48477763 MS.gene32575:CDS 45.0%
ATTGAATCCTCACCGGAGTT+TGG + chr8.4:48477708-48477727 MS.gene32575:CDS 45.0%
ATTTCTTCAGTCCAAGGGAC+AGG + chr8.4:48477105-48477124 MS.gene32575:CDS 45.0%
CTACAGCAAGAGGAGAAGTT+CGG + chr8.4:48477633-48477652 MS.gene32575:CDS 45.0%
GATTAGGAAACCAGAGTCAG+GGG + chr8.4:48477730-48477749 MS.gene32575:CDS 45.0%
GCTTCGCGAAAACACAATCT+TGG + chr8.4:48477317-48477336 MS.gene32575:CDS 45.0%
GGATTAGGAAACCAGAGTCA+GGG + chr8.4:48477729-48477748 MS.gene32575:CDS 45.0%
TACAGCAAGAGGAGAAGTTC+GGG + chr8.4:48477634-48477653 MS.gene32575:CDS 45.0%
TCACAGTTGAGTTTACCACC+TGG + chr8.4:48476609-48476628 MS.gene32575:CDS 45.0%
TCTAGACCAAACAGAGTTGC+TGG + chr8.4:48477143-48477162 MS.gene32575:CDS 45.0%
TGTGAAAGAGGATCTCTCTC+TGG - chr8.4:48476594-48476613 None:intergenic 45.0%
TTTGATCCTTGGGTTCTTCC+AGG + chr8.4:48476741-48476760 MS.gene32575:CDS 45.0%
! GAAGTTCGGGTTTCACAACA+TGG + chr8.4:48477647-48477666 MS.gene32575:CDS 45.0%
! GTGCTGGACTTTTCTCTGAT+TGG + chr8.4:48477670-48477689 MS.gene32575:CDS 45.0%
! TCTCCTCTTGCTGTAGCATT+CGG - chr8.4:48477629-48477648 None:intergenic 45.0%
! TGGATTAGGAAACCAGAGTC+AGG + chr8.4:48477728-48477747 MS.gene32575:CDS 45.0%
!!! TTATGGTTTTGGTGCAGGCA+AGG + chr8.4:48477058-48477077 MS.gene32575:intron 45.0%
AATTCAGCATCCCCTGACTC+TGG - chr8.4:48477743-48477762 None:intergenic 50.0%
ACTCCGAATGCTACAGCAAG+AGG + chr8.4:48477623-48477642 MS.gene32575:CDS 50.0%
AGTCCAACATTTCGTCCACC+TGG - chr8.4:48477554-48477573 None:intergenic 50.0%
ATCAGAGCTTTGCATTGCCG+CGG + chr8.4:48477592-48477611 MS.gene32575:CDS 50.0%
ATTCGGAGTGATGTTACCCG+CGG - chr8.4:48477612-48477631 None:intergenic 50.0%
CAAGAAGCTCCTCATCAGTG+GGG - chr8.4:48476647-48476666 None:intergenic 50.0%
CAGATTCTTCCCCACTGATG+AGG + chr8.4:48476635-48476654 MS.gene32575:CDS 50.0%
CATGTTACCAAACGGTGGCA+CGG - chr8.4:48477793-48477812 None:intergenic 50.0%
CCAGAACCAGCAACTCTGTT+TGG - chr8.4:48477152-48477171 None:intergenic 50.0%
CGAAATGTTGGACTCGTTCC+TGG + chr8.4:48477560-48477579 MS.gene32575:CDS 50.0%
GTCCAACATTTCGTCCACCT+GGG - chr8.4:48477553-48477572 None:intergenic 50.0%
TCCTAATCCAAACTCCGGTG+AGG - chr8.4:48477718-48477737 None:intergenic 50.0%
TCCTCACCGGAGTTTGGATT+AGG + chr8.4:48477714-48477733 MS.gene32575:CDS 50.0%
TCTGGTTACTGGAAAGCCAC+AGG + chr8.4:48477167-48477186 MS.gene32575:CDS 50.0%
TGGTAACATGGACTTGTCGG+CGG + chr8.4:48477803-48477822 MS.gene32575:CDS 50.0%
! GGGAAGAATCTGAAACCAGG+TGG - chr8.4:48476627-48476646 None:intergenic 50.0%
! GTGGGGAAGAATCTGAAACC+AGG - chr8.4:48476630-48476649 None:intergenic 50.0%
!! CCAAACAGAGTTGCTGGTTC+TGG + chr8.4:48477149-48477168 MS.gene32575:CDS 50.0%
!! GAGTTGCTGGTTCTGGTTAC+TGG + chr8.4:48477156-48477175 MS.gene32575:CDS 50.0%
GGGTTTCACAACATGGGTGC+TGG + chr8.4:48477654-48477673 MS.gene32575:CDS 55.0%
GTCCCAGGTGGACGAAATGT+TGG + chr8.4:48477548-48477567 MS.gene32575:CDS 55.0%
TCAGAGCTTTGCATTGCCGC+GGG + chr8.4:48477593-48477612 MS.gene32575:CDS 55.0%
TTACCAAACGGTGGCACGGA+AGG - chr8.4:48477789-48477808 None:intergenic 55.0%
! CGTGCCACCGTTTGGTAACA+TGG + chr8.4:48477791-48477810 MS.gene32575:CDS 55.0%
! GGTGCAGGCAAGGCGAATTT+TGG + chr8.4:48477068-48477087 MS.gene32575:intron 55.0%
CTCTTCGTCGGTGGGGTTCT+TGG - chr8.4:48477832-48477851 None:intergenic 60.0%
GTTCCTTCCGTGCCACCGTT+TGG + chr8.4:48477783-48477802 MS.gene32575:CDS 60.0%
TCCACCTGGGACGACGATGA+AGG - chr8.4:48477540-48477559 None:intergenic 60.0%
TTCGCCTTCATCGTCGTCCC+AGG + chr8.4:48477533-48477552 MS.gene32575:CDS 60.0%
GCCTTCATCGTCGTCCCAGG+TGG + chr8.4:48477536-48477555 MS.gene32575:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 48476572 48477851 48476572 ID=MS.gene32575
chr8.4 mRNA 48476572 48477851 48476572 ID=MS.gene32575.t1;Parent=MS.gene32575
chr8.4 exon 48476572 48476762 48476572 ID=MS.gene32575.t1.exon1;Parent=MS.gene32575.t1
chr8.4 CDS 48476572 48476762 48476572 ID=cds.MS.gene32575.t1;Parent=MS.gene32575.t1
chr8.4 exon 48477075 48477349 48477075 ID=MS.gene32575.t1.exon2;Parent=MS.gene32575.t1
chr8.4 CDS 48477075 48477349 48477075 ID=cds.MS.gene32575.t1;Parent=MS.gene32575.t1
chr8.4 exon 48477438 48477851 48477438 ID=MS.gene32575.t1.exon3;Parent=MS.gene32575.t1
chr8.4 CDS 48477438 48477851 48477438 ID=cds.MS.gene32575.t1;Parent=MS.gene32575.t1
Gene Sequence

>MS.gene32575

TTTCATCATAATGGGAGTTCCAGAGAGAGATCCTCTTTCACAGTTGAGTTTACCACCTGGTTTCAGATTCTTCCCCACTGATGAGGAGCTTCTTGTTCAGTATCTATGTCGTAAAGTTGCAGGACATCATTTTTCTTTGCCAATTATTGCTGAAATTGACTTGTACAAGTTTGATCCTTGGGTTCTTCCAGGTAATTATCACCAATTTAGGTTGCAATTGCAGAATTGTTCATGTCTTTAAATTTTAAAATATTAAATGTATGTTTGATATTTCCGTAAGTTTGTCAGAATCACGGTGAACCACTGTGATTTTGCAATAGTTACACTTTCAAGCTTGTAACTTTTGCAAGATCACACTGAAATTCTGACAATAATACGGTAATATGAAACATGCACTAAAATCACAACTATGTTAATACTGTTGCTAAATATTTTGTGTTGTTTATATTTAATTTGTTTAATTGAGATGCAATTTTAACATTGTTGTTATGGTTTTGGTGCAGGCAAGGCGAATTTTGGTGAGAAAGAATGGTATTTCTTCAGTCCAAGGGACAGGAAATATCCAAATGGTTCTAGACCAAACAGAGTTGCTGGTTCTGGTTACTGGAAAGCCACAGGAACAGACAAAATTATCACAACTGAAGGTAGAAAAGTTGGCATAAAAAAAGCACTTGTTTTCTATATTGGCAAAGCACCAAAAGGAACAAAAACTAATTGGATTATGCATGAGTATCGTCTTCTTGATGCTTCGCGAAAACACAATCTTGGTAGCACAAAAGTAAGCAACACAATCGTATTATATTTCCTTGCTCATTTTTATTGATGAGAAAAGTAAAGAATCTAACTTATTTGATTTATTGATGCAGCTTGATGATTGGGTTTTGTGTCGAATATACAAGAAAAACTCAAGCTCGCAAAAGGAAATTCCAACATTTTCAAGCAAAGAATGCAGCAATGGTTCTTCGCCTTCATCGTCGTCCCAGGTGGACGAAATGTTGGACTCGTTCCTGGAAATTGATGATCAGAGCTTTGCATTGCCGCGGGTAACATCACTCCGAATGCTACAGCAAGAGGAGAAGTTCGGGTTTCACAACATGGGTGCTGGACTTTTCTCTGATTGGGTTAACCCGACAGATATTGAATCCTCACCGGAGTTTGGATTAGGAAACCAGAGTCAGGGGATGCTGAATTACGGTTGTAATGACTATTGTGTTCCTTCCGTGCCACCGTTTGGTAACATGGACTTGTCGGCGGAGTCCAAGAACCCCACCGACGAAGAG
Protein sequence

>MS.gene32575.t1

FHHNGSSRERSSFTVEFTTWFQILPHUUGASCSVSMSUSCRTSFFFANYCUNULVQVUSLGSSRQGEFWUERMVFLQSKGQEISKWFUTKQSCWFWLLESHRNRQNYHNURUKSWHKKSTCFLYWQSTKRNKNULDYAUVSSSUCFAKTQSWUHKTUULGFVSNIQEKLKLAKGNSNIFKQRMQQWFFAFIVVPGGRNVGLVPGNUUSELCIAAGNITPNATARGEVRVSQHGCWTFLULGUPDRYUILTGVWIRKPESGDAELRLUULLCSFRATVWUHGLVGGVQEPHRRR