Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32821.t1 | XP_003603142.1 | 94.8 | 134 | 7 | 0 | 1 | 134 | 1 | 134 | 6.30E-55 | 223.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32821.t1 | Q42577 | 66.2 | 142 | 40 | 2 | 1 | 134 | 1 | 142 | 1.9e-44 | 179.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32821.t1 | G7J7M2 | 94.8 | 134 | 7 | 0 | 1 | 134 | 1 | 134 | 4.5e-55 | 223.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060634 | MS.gene32821 | 0.818731 | 1.68E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32821.t1 | MTR_3g104310 | 95.522 | 134 | 6 | 0 | 1 | 134 | 1 | 134 | 2.09e-75 | 223 |
| MS.gene32821.t1 | MTR_4g051225 | 42.222 | 90 | 51 | 1 | 46 | 134 | 3 | 92 | 1.50e-21 | 86.3 |
| MS.gene32821.t1 | MTR_7g021790 | 42.222 | 90 | 51 | 1 | 46 | 134 | 3 | 92 | 4.93e-21 | 85.1 |
| MS.gene32821.t1 | MTR_0002s0630 | 43.662 | 71 | 40 | 0 | 64 | 134 | 22 | 92 | 4.79e-19 | 81.3 |
| MS.gene32821.t1 | MTR_7g033745 | 38.889 | 90 | 53 | 2 | 46 | 134 | 3 | 91 | 4.98e-18 | 74.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32821.t1 | AT5G11770 | 71.831 | 142 | 32 | 3 | 1 | 134 | 1 | 142 | 9.67e-60 | 184 |
| MS.gene32821.t1 | ATCG00430 | 41.111 | 90 | 52 | 1 | 46 | 134 | 3 | 92 | 2.80e-20 | 83.2 |
Find 43 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGTTGATAATCTCATGAAT+TGG | 0.373372 | 3.1:-82288296 | MS.gene32821:CDS |
| AGGCGGTGGAGCGTAAGGGG+TGG | 0.374478 | 3.1:+82288377 | None:intergenic |
| AAGAGCGGGAGCCATCTTAT+TGG | 0.382683 | 3.1:+82288113 | None:intergenic |
| GGAGGAGGCGGTGGAGCGTA+AGG | 0.398546 | 3.1:+82288372 | None:intergenic |
| GTGGTCGATGGAGGAGGCGG+TGG | 0.407856 | 3.1:+82288363 | None:intergenic |
| TCAATCTGGCCTATGACTTT+CGG | 0.408619 | 3.1:-82288261 | MS.gene32821:CDS |
| TCTGATTGTATGATTGTTGC+TGG | 0.414093 | 3.1:-82288144 | MS.gene32821:CDS |
| GAGGTGGTCGATGGAGGAGG+CGG | 0.422742 | 3.1:+82288360 | None:intergenic |
| GGTTGATAATCTCATGAATT+GGG | 0.445690 | 3.1:-82288295 | MS.gene32821:CDS |
| AGGAGGCGGTGGAGCGTAAG+GGG | 0.451460 | 3.1:+82288374 | None:intergenic |
| CGTTACGATCTGGATCGATT+TGG | 0.459982 | 3.1:-82288195 | MS.gene32821:CDS |
| GGCGGTGGAGCGTAAGGGGT+GGG | 0.470168 | 3.1:+82288378 | None:intergenic |
| TGGTGCTGCTCGTTACGATC+TGG | 0.473692 | 3.1:-82288205 | MS.gene32821:CDS |
| CTCATGAATTGGGCTCGTAC+TGG | 0.482560 | 3.1:-82288285 | MS.gene32821:CDS |
| GGAGAGGTGGTCGATGGAGG+AGG | 0.486052 | 3.1:+82288357 | None:intergenic |
| CACGCGAGTCCGAAAGTCAT+AGG | 0.490407 | 3.1:+82288252 | None:intergenic |
| GTGTGGTTGATGAGAGAGAT+GGG | 0.495287 | 3.1:+82288400 | None:intergenic |
| CAATCAGATTGACGAGGACT+AGG | 0.496509 | 3.1:+82288159 | None:intergenic |
| GCCGTCTTCGATATTCCAAC+GGG | 0.500071 | 3.1:+82288336 | None:intergenic |
| TGGAGCGTAAGGGGTGGGTG+TGG | 0.502797 | 3.1:+82288383 | None:intergenic |
| GGGCTCGTACTGGCTCAATC+TGG | 0.503571 | 3.1:-82288275 | MS.gene32821:CDS |
| GAAACATACTTGCGAAGAGC+GGG | 0.507595 | 3.1:+82288099 | None:intergenic |
| CTTCGATATTCCAACGGGAG+AGG | 0.515451 | 3.1:+82288341 | None:intergenic |
| CCATCGACCACCTCTCCCGT+TGG | 0.528192 | 3.1:-82288351 | MS.gene32821:CDS |
| GAGGAGGCGGTGGAGCGTAA+GGG | 0.528912 | 3.1:+82288373 | None:intergenic |
| AGAGAGATGGGAGGGTTGTG+TGG | 0.531851 | 3.1:+82288412 | None:intergenic |
| GGTGTGGTTGATGAGAGAGA+TGG | 0.541984 | 3.1:+82288399 | None:intergenic |
| ACGGGAGAGGTGGTCGATGG+AGG | 0.553280 | 3.1:+82288354 | None:intergenic |
| CGATATTCCAACGGGAGAGG+TGG | 0.560126 | 3.1:+82288344 | None:intergenic |
| TGGTACTCTTACCAATAAGA+TGG | 0.563740 | 3.1:-82288124 | MS.gene32821:CDS |
| CGCCGTCTTCGATATTCCAA+CGG | 0.565051 | 3.1:+82288335 | None:intergenic |
| AGAAACATACTTGCGAAGAG+CGG | 0.569989 | 3.1:+82288098 | None:intergenic |
| GCTGTTGAAATGATGCATAC+TGG | 0.608176 | 3.1:-82288225 | MS.gene32821:CDS |
| TATTTGAGATAAACGAGATG+AGG | 0.609192 | 3.1:+82288461 | None:intergenic |
| CCAACGGGAGAGGTGGTCGA+TGG | 0.621156 | 3.1:+82288351 | None:intergenic |
| TCCCGTTGGAATATCGAAGA+CGG | 0.641180 | 3.1:-82288337 | MS.gene32821:CDS |
| TGGTTGATGAGAGAGATGGG+AGG | 0.644440 | 3.1:+82288403 | None:intergenic |
| GGTTGATGAGAGAGATGGGA+GGG | 0.644803 | 3.1:+82288404 | None:intergenic |
| GGCGGAGTACGTGATCTCGA+AGG | 0.687555 | 3.1:-82288316 | MS.gene32821:CDS |
| CGTTGGAATATCGAAGACGG+CGG | 0.718228 | 3.1:-82288334 | MS.gene32821:CDS |
| AGAAACGCATCGTTGCGGTG+AGG | 0.731636 | 3.1:+82288437 | None:intergenic |
| ATCATACAATCAGATTGACG+AGG | 0.742801 | 3.1:+82288153 | None:intergenic |
| TGGAGAGAAACGCATCGTTG+CGG | 0.767423 | 3.1:+82288432 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AACAAATCAATGCTTCTATA+AGG | + | chr3.1:82288455-82288474 | None:intergenic | 25.0% |
| ! | ACAAATCAATGCTTCTATAA+GGG | + | chr3.1:82288454-82288473 | None:intergenic | 25.0% |
| ATGTAAAAACCCTAGAAAAG+AGG | + | chr3.1:82288395-82288414 | None:intergenic | 30.0% | |
| TGTAAAAACCCTAGAAAAGA+GGG | + | chr3.1:82288394-82288413 | None:intergenic | 30.0% | |
| ! | TATTTGAGATAAACGAGATG+AGG | + | chr3.1:82288005-82288024 | None:intergenic | 30.0% |
| ! | TTTCTTTTCCCTCTTTTCTA+GGG | - | chr3.1:82288383-82288402 | MS.gene32821:CDS | 30.0% |
| !! | AGGTTGATAATCTCATGAAT+TGG | - | chr3.1:82288167-82288186 | MS.gene32821:CDS | 30.0% |
| !! | GGTTGATAATCTCATGAATT+GGG | - | chr3.1:82288168-82288187 | MS.gene32821:CDS | 30.0% |
| ATCATACAATCAGATTGACG+AGG | + | chr3.1:82288313-82288332 | None:intergenic | 35.0% | |
| TCTGATTGTATGATTGTTGC+TGG | - | chr3.1:82288319-82288338 | MS.gene32821:CDS | 35.0% | |
| TGGTACTCTTACCAATAAGA+TGG | - | chr3.1:82288339-82288358 | MS.gene32821:CDS | 35.0% | |
| ! | GTTTCTTTTCCCTCTTTTCT+AGG | - | chr3.1:82288382-82288401 | MS.gene32821:CDS | 35.0% |
| AGAAACATACTTGCGAAGAG+CGG | + | chr3.1:82288368-82288387 | None:intergenic | 40.0% | |
| GCTGTTGAAATGATGCATAC+TGG | - | chr3.1:82288238-82288257 | MS.gene32821:CDS | 40.0% | |
| TCAATCTGGCCTATGACTTT+CGG | - | chr3.1:82288202-82288221 | MS.gene32821:CDS | 40.0% | |
| CAATCAGATTGACGAGGACT+AGG | + | chr3.1:82288307-82288326 | None:intergenic | 45.0% | |
| CGTTACGATCTGGATCGATT+TGG | - | chr3.1:82288268-82288287 | MS.gene32821:CDS | 45.0% | |
| GAAACATACTTGCGAAGAGC+GGG | + | chr3.1:82288367-82288386 | None:intergenic | 45.0% | |
| TCCCGTTGGAATATCGAAGA+CGG | - | chr3.1:82288126-82288145 | MS.gene32821:CDS | 45.0% | |
| !! | GTGTGGTTGATGAGAGAGAT+GGG | + | chr3.1:82288066-82288085 | None:intergenic | 45.0% |
| AAGAGCGGGAGCCATCTTAT+TGG | + | chr3.1:82288353-82288372 | None:intergenic | 50.0% | |
| CGCCGTCTTCGATATTCCAA+CGG | + | chr3.1:82288131-82288150 | None:intergenic | 50.0% | |
| CGTTGGAATATCGAAGACGG+CGG | - | chr3.1:82288129-82288148 | MS.gene32821:CDS | 50.0% | |
| CTCATGAATTGGGCTCGTAC+TGG | - | chr3.1:82288178-82288197 | MS.gene32821:CDS | 50.0% | |
| CTTCGATATTCCAACGGGAG+AGG | + | chr3.1:82288125-82288144 | None:intergenic | 50.0% | |
| GCCGTCTTCGATATTCCAAC+GGG | + | chr3.1:82288130-82288149 | None:intergenic | 50.0% | |
| TGGAGAGAAACGCATCGTTG+CGG | + | chr3.1:82288034-82288053 | None:intergenic | 50.0% | |
| !! | GGTGTGGTTGATGAGAGAGA+TGG | + | chr3.1:82288067-82288086 | None:intergenic | 50.0% |
| !! | GGTTGATGAGAGAGATGGGA+GGG | + | chr3.1:82288062-82288081 | None:intergenic | 50.0% |
| !! | TGGTTGATGAGAGAGATGGG+AGG | + | chr3.1:82288063-82288082 | None:intergenic | 50.0% |
| AGAAACGCATCGTTGCGGTG+AGG | + | chr3.1:82288029-82288048 | None:intergenic | 55.0% | |
| AGAGAGATGGGAGGGTTGTG+TGG | + | chr3.1:82288054-82288073 | None:intergenic | 55.0% | |
| CACGCGAGTCCGAAAGTCAT+AGG | + | chr3.1:82288214-82288233 | None:intergenic | 55.0% | |
| CGATATTCCAACGGGAGAGG+TGG | + | chr3.1:82288122-82288141 | None:intergenic | 55.0% | |
| !! | TGGTGCTGCTCGTTACGATC+TGG | - | chr3.1:82288258-82288277 | MS.gene32821:CDS | 55.0% |
| GGCGGAGTACGTGATCTCGA+AGG | - | chr3.1:82288147-82288166 | MS.gene32821:CDS | 60.0% | |
| !! | GGGCTCGTACTGGCTCAATC+TGG | - | chr3.1:82288188-82288207 | MS.gene32821:CDS | 60.0% |
| ACGGGAGAGGTGGTCGATGG+AGG | + | chr3.1:82288112-82288131 | None:intergenic | 65.0% | |
| AGGAGGCGGTGGAGCGTAAG+GGG | + | chr3.1:82288092-82288111 | None:intergenic | 65.0% | |
| CCAACGGGAGAGGTGGTCGA+TGG | + | chr3.1:82288115-82288134 | None:intergenic | 65.0% | |
| CCATCGACCACCTCTCCCGT+TGG | - | chr3.1:82288112-82288131 | MS.gene32821:CDS | 65.0% | |
| GAGGAGGCGGTGGAGCGTAA+GGG | + | chr3.1:82288093-82288112 | None:intergenic | 65.0% | |
| GAGGTGGTCGATGGAGGAGG+CGG | + | chr3.1:82288106-82288125 | None:intergenic | 65.0% | |
| GGAGAGGTGGTCGATGGAGG+AGG | + | chr3.1:82288109-82288128 | None:intergenic | 65.0% | |
| TGGAGCGTAAGGGGTGGGTG+TGG | + | chr3.1:82288083-82288102 | None:intergenic | 65.0% | |
| AGGCGGTGGAGCGTAAGGGG+TGG | + | chr3.1:82288089-82288108 | None:intergenic | 70.0% | |
| GGAGGAGGCGGTGGAGCGTA+AGG | + | chr3.1:82288094-82288113 | None:intergenic | 70.0% | |
| GGCGGTGGAGCGTAAGGGGT+GGG | + | chr3.1:82288088-82288107 | None:intergenic | 70.0% | |
| GTGGTCGATGGAGGAGGCGG+TGG | + | chr3.1:82288103-82288122 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 82287980 | 82288505 | 82287980 | ID=MS.gene32821 |
| chr3.1 | mRNA | 82287980 | 82288505 | 82287980 | ID=MS.gene32821.t1;Parent=MS.gene32821 |
| chr3.1 | exon | 82288108 | 82288505 | 82288108 | ID=MS.gene32821.t1.exon1;Parent=MS.gene32821.t1 |
| chr3.1 | CDS | 82288108 | 82288505 | 82288108 | ID=cds.MS.gene32821.t1;Parent=MS.gene32821.t1 |
| chr3.1 | exon | 82287980 | 82287986 | 82287980 | ID=MS.gene32821.t1.exon2;Parent=MS.gene32821.t1 |
| chr3.1 | CDS | 82287980 | 82287986 | 82287980 | ID=cds.MS.gene32821.t1;Parent=MS.gene32821.t1 |
| Gene Sequence |
| Protein sequence |
