Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32916.t1 | XP_013461730.1 | 95.2 | 373 | 17 | 1 | 1 | 373 | 1 | 372 | 1.50E-202 | 715.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32916.t1 | Q9FMH5 | 51.5 | 357 | 157 | 5 | 3 | 359 | 12 | 352 | 3.8e-98 | 359.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32916.t1 | A0A072VC73 | 95.2 | 373 | 17 | 1 | 1 | 373 | 1 | 372 | 1.1e-202 | 715.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054662 | MS.gene32916 | 0.809467 | 1.87E-50 | -1.69E-46 |
| MS.gene06093 | MS.gene32916 | 0.809383 | 1.94E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32916.t1 | MTR_3g102520 | 95.174 | 373 | 17 | 1 | 1 | 373 | 1 | 372 | 0.0 | 738 |
| MS.gene32916.t1 | MTR_3g102510 | 80.833 | 360 | 49 | 3 | 1 | 360 | 1 | 340 | 0.0 | 595 |
| MS.gene32916.t1 | MTR_3g102530 | 70.000 | 360 | 100 | 3 | 1 | 360 | 1 | 352 | 3.00e-177 | 496 |
| MS.gene32916.t1 | MTR_1g018680 | 53.959 | 341 | 138 | 4 | 24 | 358 | 30 | 357 | 1.28e-122 | 358 |
| MS.gene32916.t1 | MTR_3g102560 | 51.253 | 359 | 137 | 6 | 1 | 357 | 2 | 324 | 1.76e-110 | 326 |
| MS.gene32916.t1 | MTR_3g102540 | 54.580 | 262 | 113 | 2 | 96 | 357 | 13 | 268 | 3.67e-95 | 285 |
| MS.gene32916.t1 | MTR_1g011470 | 45.221 | 272 | 147 | 1 | 79 | 350 | 148 | 417 | 4.70e-79 | 249 |
| MS.gene32916.t1 | MTR_3g107970 | 45.221 | 272 | 147 | 1 | 79 | 350 | 200 | 469 | 3.24e-78 | 248 |
| MS.gene32916.t1 | MTR_3g088415 | 45.588 | 272 | 146 | 1 | 87 | 358 | 235 | 504 | 6.40e-78 | 248 |
| MS.gene32916.t1 | MTR_3g088405 | 43.234 | 303 | 156 | 3 | 57 | 357 | 220 | 508 | 1.63e-77 | 248 |
| MS.gene32916.t1 | MTR_2g102520 | 45.105 | 286 | 152 | 3 | 74 | 357 | 197 | 479 | 7.89e-77 | 245 |
| MS.gene32916.t1 | MTR_2g102550 | 43.011 | 279 | 156 | 2 | 79 | 357 | 154 | 429 | 1.09e-72 | 233 |
| MS.gene32916.t1 | MTR_3g088415 | 48.558 | 208 | 105 | 1 | 87 | 294 | 235 | 440 | 3.74e-64 | 211 |
| MS.gene32916.t1 | MTR_3g088415 | 51.634 | 153 | 72 | 1 | 87 | 239 | 235 | 385 | 1.39e-47 | 166 |
| MS.gene32916.t1 | MTR_8g074000 | 33.716 | 261 | 164 | 4 | 84 | 344 | 168 | 419 | 1.04e-44 | 159 |
| MS.gene32916.t1 | MTR_7g089080 | 35.842 | 279 | 171 | 7 | 72 | 347 | 177 | 450 | 1.80e-44 | 164 |
| MS.gene32916.t1 | MTR_5g088980 | 34.646 | 254 | 156 | 5 | 83 | 335 | 156 | 400 | 2.38e-43 | 155 |
| MS.gene32916.t1 | MTR_5g023790 | 34.766 | 256 | 158 | 4 | 89 | 344 | 169 | 415 | 1.44e-42 | 154 |
| MS.gene32916.t1 | MTR_5g023790 | 37.727 | 220 | 127 | 5 | 89 | 308 | 169 | 378 | 1.13e-38 | 142 |
| MS.gene32916.t1 | MTR_5g023790 | 40.385 | 156 | 90 | 2 | 89 | 244 | 169 | 321 | 1.73e-29 | 117 |
| MS.gene32916.t1 | MTR_3g060770 | 32.328 | 232 | 136 | 7 | 127 | 345 | 60 | 283 | 3.83e-27 | 109 |
| MS.gene32916.t1 | MTR_3g060410 | 35.323 | 201 | 121 | 6 | 123 | 318 | 131 | 327 | 4.33e-27 | 110 |
| MS.gene32916.t1 | MTR_3g060400 | 28.873 | 284 | 178 | 10 | 76 | 345 | 5 | 278 | 8.84e-25 | 102 |
| MS.gene32916.t1 | MTR_3g460990 | 28.873 | 284 | 178 | 10 | 76 | 345 | 5 | 278 | 8.84e-25 | 102 |
| MS.gene32916.t1 | MTR_3g060820 | 31.897 | 232 | 138 | 8 | 126 | 345 | 49 | 272 | 9.46e-25 | 102 |
| MS.gene32916.t1 | MTR_3g061680 | 34.500 | 200 | 114 | 7 | 126 | 316 | 49 | 240 | 2.63e-24 | 101 |
| MS.gene32916.t1 | MTR_5g044040 | 46.667 | 75 | 40 | 0 | 149 | 223 | 7 | 81 | 3.46e-16 | 74.7 |
| MS.gene32916.t1 | MTR_8g063120 | 31.250 | 176 | 104 | 7 | 103 | 265 | 68 | 239 | 2.04e-12 | 67.4 |
| MS.gene32916.t1 | MTR_8g063120 | 31.250 | 176 | 104 | 7 | 103 | 265 | 68 | 239 | 3.75e-12 | 67.4 |
| MS.gene32916.t1 | MTR_5g011900 | 28.082 | 146 | 104 | 1 | 120 | 265 | 95 | 239 | 5.00e-11 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32916.t1 | AT5G43080 | 51.541 | 357 | 157 | 5 | 3 | 359 | 12 | 352 | 6.22e-129 | 374 |
| MS.gene32916.t1 | AT5G43080 | 50.700 | 357 | 155 | 6 | 3 | 359 | 12 | 347 | 1.52e-125 | 365 |
| MS.gene32916.t1 | AT1G47230 | 49.171 | 362 | 169 | 5 | 3 | 359 | 11 | 362 | 5.91e-122 | 357 |
| MS.gene32916.t1 | AT1G47210 | 48.361 | 366 | 173 | 3 | 3 | 359 | 11 | 369 | 1.44e-120 | 353 |
| MS.gene32916.t1 | AT1G47230 | 49.036 | 363 | 169 | 6 | 3 | 359 | 11 | 363 | 4.77e-120 | 352 |
| MS.gene32916.t1 | AT1G47220 | 55.124 | 283 | 127 | 0 | 77 | 359 | 41 | 323 | 1.09e-107 | 319 |
| MS.gene32916.t1 | AT1G80370 | 46.241 | 266 | 140 | 2 | 92 | 357 | 197 | 459 | 1.44e-77 | 246 |
| MS.gene32916.t1 | AT1G44110 | 45.520 | 279 | 150 | 1 | 79 | 357 | 181 | 457 | 2.88e-77 | 245 |
| MS.gene32916.t1 | AT5G11300 | 45.745 | 282 | 139 | 2 | 67 | 348 | 159 | 426 | 5.67e-77 | 244 |
| MS.gene32916.t1 | AT1G15570 | 45.865 | 266 | 141 | 2 | 92 | 357 | 188 | 450 | 4.08e-75 | 239 |
| MS.gene32916.t1 | AT5G25380 | 43.369 | 279 | 155 | 2 | 79 | 357 | 160 | 435 | 4.58e-73 | 234 |
| MS.gene32916.t1 | AT5G25380 | 43.369 | 279 | 155 | 2 | 79 | 357 | 160 | 435 | 4.58e-73 | 234 |
| MS.gene32916.t1 | AT5G25380 | 43.369 | 279 | 155 | 2 | 79 | 357 | 166 | 441 | 6.03e-73 | 234 |
| MS.gene32916.t1 | AT5G25380 | 42.748 | 262 | 148 | 1 | 79 | 340 | 160 | 419 | 2.41e-69 | 224 |
| MS.gene32916.t1 | AT1G77390 | 40.893 | 291 | 158 | 2 | 67 | 357 | 165 | 441 | 5.07e-69 | 224 |
| MS.gene32916.t1 | AT5G11300 | 46.154 | 247 | 110 | 3 | 67 | 304 | 159 | 391 | 3.81e-65 | 213 |
| MS.gene32916.t1 | AT2G26760 | 33.550 | 307 | 180 | 5 | 43 | 348 | 95 | 378 | 1.08e-44 | 158 |
| MS.gene32916.t1 | AT5G06150 | 35.273 | 275 | 167 | 6 | 71 | 344 | 160 | 424 | 1.13e-44 | 160 |
| MS.gene32916.t1 | AT4G37490 | 36.641 | 262 | 150 | 7 | 92 | 347 | 165 | 416 | 2.02e-44 | 159 |
| MS.gene32916.t1 | AT3G11520 | 36.641 | 262 | 156 | 5 | 83 | 344 | 150 | 401 | 2.83e-44 | 158 |
| MS.gene32916.t1 | AT3G11520 | 38.723 | 235 | 134 | 5 | 83 | 317 | 150 | 374 | 2.54e-42 | 152 |
| MS.gene32916.t1 | AT1G76310 | 32.740 | 281 | 179 | 5 | 67 | 346 | 152 | 423 | 3.80e-41 | 150 |
| MS.gene32916.t1 | AT1G20610 | 33.333 | 249 | 157 | 4 | 92 | 340 | 177 | 416 | 1.40e-40 | 148 |
| MS.gene32916.t1 | AT1G16330 | 31.095 | 283 | 167 | 6 | 58 | 335 | 370 | 629 | 2.02e-39 | 148 |
| MS.gene32916.t1 | AT1G47210 | 41.799 | 189 | 94 | 3 | 3 | 182 | 11 | 192 | 1.18e-38 | 137 |
| MS.gene32916.t1 | AT1G76310 | 34.016 | 244 | 151 | 5 | 67 | 309 | 152 | 386 | 4.11e-37 | 138 |
| MS.gene32916.t1 | AT4G35620 | 37.891 | 256 | 144 | 7 | 92 | 344 | 172 | 415 | 1.28e-36 | 137 |
| MS.gene32916.t1 | AT1G76310 | 30.097 | 309 | 178 | 6 | 67 | 346 | 152 | 451 | 8.25e-36 | 136 |
| MS.gene32916.t1 | AT2G17620 | 36.900 | 271 | 155 | 7 | 92 | 361 | 171 | 426 | 1.07e-35 | 135 |
| MS.gene32916.t1 | AT4G35620 | 41.232 | 211 | 110 | 6 | 92 | 300 | 172 | 370 | 1.51e-34 | 131 |
| MS.gene32916.t1 | AT5G06150 | 40.719 | 167 | 94 | 4 | 71 | 236 | 160 | 322 | 9.75e-31 | 120 |
| MS.gene32916.t1 | AT1G76310 | 35.556 | 180 | 112 | 3 | 66 | 244 | 151 | 327 | 4.97e-28 | 112 |
| MS.gene32916.t1 | AT1G20590 | 37.342 | 158 | 91 | 4 | 156 | 312 | 66 | 216 | 1.04e-25 | 104 |
| MS.gene32916.t1 | AT1G14750 | 28.017 | 232 | 143 | 9 | 62 | 283 | 155 | 372 | 2.65e-14 | 74.3 |
| MS.gene32916.t1 | AT1G14750 | 28.017 | 232 | 143 | 9 | 62 | 283 | 323 | 540 | 5.93e-14 | 73.6 |
| MS.gene32916.t1 | AT1G14750 | 28.017 | 232 | 143 | 9 | 62 | 283 | 323 | 540 | 6.15e-14 | 73.6 |
| MS.gene32916.t1 | AT1G14750 | 28.017 | 232 | 142 | 10 | 62 | 283 | 155 | 371 | 4.31e-13 | 70.5 |
| MS.gene32916.t1 | AT1G34460 | 30.539 | 167 | 91 | 5 | 71 | 236 | 236 | 378 | 7.57e-13 | 70.1 |
| MS.gene32916.t1 | AT1G34460 | 30.539 | 167 | 91 | 5 | 71 | 236 | 250 | 392 | 7.85e-13 | 70.1 |
| MS.gene32916.t1 | AT1G34460 | 30.539 | 167 | 91 | 5 | 71 | 236 | 250 | 392 | 7.85e-13 | 70.1 |
| MS.gene32916.t1 | AT1G34460 | 30.539 | 167 | 91 | 5 | 71 | 236 | 250 | 392 | 7.85e-13 | 70.1 |
| MS.gene32916.t1 | AT1G34460 | 30.539 | 167 | 91 | 5 | 71 | 236 | 250 | 392 | 7.85e-13 | 70.1 |
| MS.gene32916.t1 | AT1G34460 | 30.539 | 167 | 91 | 5 | 71 | 236 | 250 | 392 | 9.86e-13 | 69.7 |
| MS.gene32916.t1 | AT1G34460 | 30.539 | 167 | 91 | 5 | 71 | 236 | 250 | 392 | 1.07e-12 | 69.7 |
| MS.gene32916.t1 | AT1G34460 | 30.539 | 167 | 91 | 5 | 71 | 236 | 247 | 389 | 1.19e-12 | 69.3 |
| MS.gene32916.t1 | AT1G34460 | 30.539 | 167 | 91 | 5 | 71 | 236 | 247 | 389 | 1.19e-12 | 69.3 |
Find 84 sgRNAs with CRISPR-Local
Find 138 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCACGTTTGGCATTGATTT+TGG | 0.171036 | 3.1:+81348390 | None:intergenic |
| TAAAACAATCCTCTGTCTTT+TGG | 0.193245 | 3.1:+81348552 | None:intergenic |
| TTAAGAAATGTTATGACATT+AGG | 0.245490 | 3.1:+81347231 | None:intergenic |
| ATGAACCGGCTTATTCAAAT+TGG | 0.248642 | 3.1:+81348414 | None:intergenic |
| TCCCTTTCAGGAGGTTTATT+GGG | 0.251091 | 3.1:-81347116 | MS.gene32916:intron |
| CATACGTGTTATCAGTAATT+TGG | 0.257271 | 3.1:+81347431 | None:intergenic |
| GGTTGCCCATTTCAAAGTTT+AGG | 0.298700 | 3.1:+81347253 | None:intergenic |
| TAAGAAATGTTATGACATTA+GGG | 0.300153 | 3.1:+81347232 | None:intergenic |
| CAGATATTCAAACTGCAAAT+TGG | 0.300324 | 3.1:+81346979 | None:intergenic |
| GTTGCCCATTTCAAAGTTTA+GGG | 0.302240 | 3.1:+81347254 | None:intergenic |
| TAAGTCCCTAAACTTTGAAA+TGG | 0.303906 | 3.1:-81347259 | MS.gene32916:CDS |
| TTCCCTTTCAGGAGGTTTAT+TGG | 0.306403 | 3.1:-81347117 | MS.gene32916:intron |
| TCTTGCTGACTTAAGCTTGT+TGG | 0.319462 | 3.1:-81346948 | MS.gene32916:CDS |
| GTGGCTGAATTCTTGAGGTT+AGG | 0.334651 | 3.1:+81348457 | None:intergenic |
| TGGCTGAATTCTTGAGGTTA+GGG | 0.348228 | 3.1:+81348458 | None:intergenic |
| CTTATTCAAATTGGAATCAA+TGG | 0.369939 | 3.1:+81348423 | None:intergenic |
| GACTTGTGGTGGTTGTTGAT+TGG | 0.372479 | 3.1:+81348582 | None:intergenic |
| ATAGGATGTTTACCTCTTCC+AGG | 0.372716 | 3.1:+81347407 | None:intergenic |
| ACTGACAGATGAAGCGTCTC+TGG | 0.379014 | 3.1:+81348080 | None:intergenic |
| TGAGAGGGACATTGGTTGAT+TGG | 0.379637 | 3.1:-81348136 | MS.gene32916:CDS |
| TCCGTCCCTGTGTGAATGCT+TGG | 0.381462 | 3.1:-81346746 | MS.gene32916:CDS |
| CTCCATTGTGCGAAGATAAT+CGG | 0.397103 | 3.1:+81348330 | None:intergenic |
| AATCTGTTGATTATCACGTT+TGG | 0.400606 | 3.1:+81348378 | None:intergenic |
| TTCTTGCCAGATTTATTATC+CGG | 0.409058 | 3.1:-81346874 | MS.gene32916:CDS |
| TGGTTGTTGATTGGTGTTGT+TGG | 0.411534 | 3.1:+81348591 | None:intergenic |
| TCCAGGCCGGATAATAAATC+TGG | 0.432284 | 3.1:+81346868 | None:intergenic |
| TGGCAGAAATCCACTGCCTT+CGG | 0.447465 | 3.1:+81347451 | None:intergenic |
| ATGTAGTACAATTAACTTTG+TGG | 0.450273 | 3.1:-81346460 | MS.gene32916:CDS |
| TTTGTCACGAACAGCTTTGA+AGG | 0.454235 | 3.1:+81346651 | None:intergenic |
| AAGTAATGATTCGGTATCTC+TGG | 0.454323 | 3.1:+81346495 | None:intergenic |
| TCCGGCCTGGAGTACATCCT+TGG | 0.454728 | 3.1:-81346856 | MS.gene32916:CDS |
| TCTTCTTCAAAGTAATGATT+CGG | 0.456454 | 3.1:+81346486 | None:intergenic |
| AAGTCCCTAAACTTTGAAAT+GGG | 0.462848 | 3.1:-81347258 | MS.gene32916:CDS |
| GCCATCTGTTGTCGCTGCAT+CGG | 0.466961 | 3.1:-81346903 | MS.gene32916:CDS |
| TGCTGACAGGTTCAATAGAT+AGG | 0.467250 | 3.1:+81348039 | None:intergenic |
| GCCAGATTTATTATCCGGCC+TGG | 0.470239 | 3.1:-81346869 | MS.gene32916:CDS |
| ATTGGTTGATTGGTTAGTTG+AGG | 0.479448 | 3.1:-81348126 | MS.gene32916:CDS |
| GTTGTTGGCTCTCTTCTTTG+AGG | 0.481908 | 3.1:+81348606 | None:intergenic |
| TGATTCCAATTTGAATAAGC+CGG | 0.489108 | 3.1:-81348419 | MS.gene32916:CDS |
| GTATCTCTGGCGATGAAGGC+AGG | 0.491666 | 3.1:+81346508 | None:intergenic |
| TTCGGTATCTCTGGCGATGA+AGG | 0.497141 | 3.1:+81346504 | None:intergenic |
| CCGTCCCTGTGTGAATGCTT+GGG | 0.501028 | 3.1:-81346745 | MS.gene32916:CDS |
| ATGATTTGTATATGTCAAGA+AGG | 0.502659 | 3.1:-81346681 | MS.gene32916:CDS |
| AGAAATCCACTGCCTTCGGT+GGG | 0.504257 | 3.1:+81347455 | None:intergenic |
| CAGAAATCCACTGCCTTCGG+TGG | 0.510454 | 3.1:+81347454 | None:intergenic |
| CTACATTGGAATTTCTGAGT+GGG | 0.514342 | 3.1:+81348487 | None:intergenic |
| AATTGGAATCAATGGAAGGA+AGG | 0.516712 | 3.1:+81348431 | None:intergenic |
| AGATCTAAGCTTCAGCTGCT+TGG | 0.517791 | 3.1:-81348017 | MS.gene32916:CDS |
| CCGGCCTGGAGTACATCCTT+GGG | 0.517846 | 3.1:-81346855 | MS.gene32916:intron |
| TTGTATATGTCAAGAAGGGC+AGG | 0.519915 | 3.1:-81346676 | MS.gene32916:CDS |
| TTCAAATTGGAATCAATGGA+AGG | 0.530972 | 3.1:+81348427 | None:intergenic |
| ATCAAACATGAGAGGGACAT+TGG | 0.531177 | 3.1:-81348144 | MS.gene32916:CDS |
| CCACTCAGAAATTCCAATGT+AGG | 0.535303 | 3.1:-81348486 | MS.gene32916:CDS |
| AATCACCCCACCGAAGGCAG+TGG | 0.543643 | 3.1:-81347461 | MS.gene32916:CDS |
| TCGGAGATATCAGATACATA+AGG | 0.545360 | 3.1:+81348349 | None:intergenic |
| ACCGATGCAGCGACAACAGA+TGG | 0.555704 | 3.1:+81346902 | None:intergenic |
| TCTGGAACAGTTAAAATGTG+TGG | 0.557657 | 3.1:-81346535 | MS.gene32916:intron |
| ATTGGAATCAATGGAAGGAA+GGG | 0.563267 | 3.1:+81348432 | None:intergenic |
| GGGTTGTGGCTGAATTCTTG+AGG | 0.568858 | 3.1:+81348452 | None:intergenic |
| CCCAAGGATGTACTCCAGGC+CGG | 0.572797 | 3.1:+81346855 | None:intergenic |
| TGAAGCTTAGATCTGCTGAC+AGG | 0.573991 | 3.1:+81348026 | None:intergenic |
| TTTACCCAAGGATGTACTCC+AGG | 0.575735 | 3.1:+81346851 | None:intergenic |
| TCAACAACCACCACAAGTCA+TGG | 0.576674 | 3.1:-81348578 | MS.gene32916:CDS |
| CTCCGATTATCTTCGCACAA+TGG | 0.576963 | 3.1:-81348332 | MS.gene32916:CDS |
| TACTGATAACACGTATGACC+TGG | 0.578166 | 3.1:-81347425 | MS.gene32916:CDS |
| ATCAATGGAAGGAAGGGTTG+TGG | 0.587426 | 3.1:+81348438 | None:intergenic |
| TTGGGAACCATGACTTGTGG+TGG | 0.593041 | 3.1:+81348571 | None:intergenic |
| TGATTTGTATATGTCAAGAA+GGG | 0.596859 | 3.1:-81346680 | MS.gene32916:CDS |
| TTCTCAAGATAACCAACCAT+TGG | 0.599787 | 3.1:+81348185 | None:intergenic |
| CCTACATTGGAATTTCTGAG+TGG | 0.604544 | 3.1:+81348486 | None:intergenic |
| CGATTATCTTCGCACAATGG+AGG | 0.617827 | 3.1:-81348329 | MS.gene32916:intron |
| TATCTTGAGAATGTTCAAAG+AGG | 0.624395 | 3.1:-81348173 | MS.gene32916:CDS |
| GCATTACATCAAACATGAGA+GGG | 0.627034 | 3.1:-81348151 | MS.gene32916:CDS |
| GGCATTACATCAAACATGAG+AGG | 0.629142 | 3.1:-81348152 | MS.gene32916:CDS |
| GATTGCTTGTGAGAATCGAA+AGG | 0.629577 | 3.1:-81347094 | MS.gene32916:intron |
| TGAAGAAATCACCCCACCGA+AGG | 0.631915 | 3.1:-81347467 | MS.gene32916:CDS |
| CCCAAGCATTCACACAGGGA+CGG | 0.632875 | 3.1:+81346745 | None:intergenic |
| GAAATCCACTGCCTTCGGTG+GGG | 0.635951 | 3.1:+81347456 | None:intergenic |
| TAACACGTATGACCTGGAAG+AGG | 0.660847 | 3.1:-81347419 | MS.gene32916:intron |
| TGACAAATACAAGCAGAACA+AGG | 0.662422 | 3.1:-81346635 | MS.gene32916:intron |
| GTAACCCAAGCATTCACACA+GGG | 0.699025 | 3.1:+81346741 | None:intergenic |
| TGTAACCCAAGCATTCACAC+AGG | 0.705344 | 3.1:+81346740 | None:intergenic |
| TCATGGTTCCCAAAAGACAG+AGG | 0.710281 | 3.1:-81348561 | MS.gene32916:CDS |
| TGAATGCTTGGGTTACACGT+CGG | 0.724505 | 3.1:-81346734 | MS.gene32916:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAACAATTAATCAAGAAG+AGG | + | chr3.1:81346764-81346783 | None:intergenic | 20.0% |
| !! | AGAGATTAATGAATTTGATA+GGG | - | chr3.1:81347422-81347441 | MS.gene32916:CDS | 20.0% |
| !! | ATAACAATTAATCAAGAAGA+GGG | + | chr3.1:81346763-81346782 | None:intergenic | 20.0% |
| !! | ATAGATGATAGAACATTAAT+GGG | - | chr3.1:81347450-81347469 | MS.gene32916:CDS | 20.0% |
| !! | ATCAAATTCATTAATCTCTA+AGG | + | chr3.1:81347421-81347440 | None:intergenic | 20.0% |
| !! | TAAGAAATGTTATGACATTA+GGG | + | chr3.1:81347833-81347852 | None:intergenic | 20.0% |
| !! | TAGAGATTAATGAATTTGAT+AGG | - | chr3.1:81347421-81347440 | MS.gene32916:CDS | 20.0% |
| !! | TCAAATTCATTAATCTCTAA+GGG | + | chr3.1:81347420-81347439 | None:intergenic | 20.0% |
| !! | TTAAGAAATGTTATGACATT+AGG | + | chr3.1:81347834-81347853 | None:intergenic | 20.0% |
| !!! | TATTTTTGTATAAGCTATCT+TGG | - | chr3.1:81347268-81347287 | MS.gene32916:CDS | 20.0% |
| !!! | TGTTATTTTTACAAAGATCT+AGG | - | chr3.1:81347136-81347155 | MS.gene32916:intron | 20.0% |
| ! | ACTTTCTGCATATTTAAAAG+TGG | + | chr3.1:81347574-81347593 | None:intergenic | 25.0% |
| ! | ATGTAGTACAATTAACTTTG+TGG | - | chr3.1:81348602-81348621 | MS.gene32916:CDS | 25.0% |
| ! | CTTATTCAAATTGGAATCAA+TGG | + | chr3.1:81346642-81346661 | None:intergenic | 25.0% |
| ! | GATAGATGATAGAACATTAA+TGG | - | chr3.1:81347449-81347468 | MS.gene32916:CDS | 25.0% |
| ! | TAACAACAAATATTTACCCA+AGG | + | chr3.1:81348226-81348245 | None:intergenic | 25.0% |
| ! | TAGATGATAGAACATTAATG+GGG | - | chr3.1:81347451-81347470 | MS.gene32916:CDS | 25.0% |
| ! | TCTTCTTCAAAGTAATGATT+CGG | + | chr3.1:81348579-81348598 | None:intergenic | 25.0% |
| !! | ATGATTTGTATATGTCAAGA+AGG | - | chr3.1:81348381-81348400 | MS.gene32916:CDS | 25.0% |
| !! | TGATTTGTATATGTCAAGAA+GGG | - | chr3.1:81348382-81348401 | MS.gene32916:CDS | 25.0% |
| !!! | ACATTGTTCACATGTTATTT+TGG | + | chr3.1:81347313-81347332 | None:intergenic | 25.0% |
| !!! | CATTGTTCACATGTTATTTT+GGG | + | chr3.1:81347312-81347331 | None:intergenic | 25.0% |
| !!! | TGGGTAAATATTTGTTGTTA+TGG | - | chr3.1:81348226-81348245 | MS.gene32916:intron | 25.0% |
| AAAAACAGGAAGATTGAGAT+CGG | + | chr3.1:81346546-81346565 | None:intergenic | 30.0% | |
| ACAATTAGTAAACACAACAC+AGG | + | chr3.1:81348040-81348059 | None:intergenic | 30.0% | |
| ATAACAGTTGCACATAAACA+TGG | + | chr3.1:81347923-81347942 | None:intergenic | 30.0% | |
| ATAAGCTATTTACCCAAACA+AGG | + | chr3.1:81347162-81347181 | None:intergenic | 30.0% | |
| CAGATATTCAAACTGCAAAT+TGG | + | chr3.1:81348086-81348105 | None:intergenic | 30.0% | |
| CATACGTGTTATCAGTAATT+TGG | + | chr3.1:81347634-81347653 | None:intergenic | 30.0% | |
| TATCTTGAGAATGTTCAAAG+AGG | - | chr3.1:81346889-81346908 | MS.gene32916:CDS | 30.0% | |
| TGATTCCAATTTGAATAAGC+CGG | - | chr3.1:81346643-81346662 | MS.gene32916:CDS | 30.0% | |
| TTCAAATTGGAATCAATGGA+AGG | + | chr3.1:81346638-81346657 | None:intergenic | 30.0% | |
| TTCTTGCCAGATTTATTATC+CGG | - | chr3.1:81348188-81348207 | MS.gene32916:CDS | 30.0% | |
| ! | AAAACAATCCTCTGTCTTTT+GGG | + | chr3.1:81346512-81346531 | None:intergenic | 30.0% |
| ! | AAAAGACAGAGGATTGTTTT+AGG | - | chr3.1:81346512-81346531 | MS.gene32916:CDS | 30.0% |
| ! | AATCTGTTGATTATCACGTT+TGG | + | chr3.1:81346687-81346706 | None:intergenic | 30.0% |
| ! | TAAAACAATCCTCTGTCTTT+TGG | + | chr3.1:81346513-81346532 | None:intergenic | 30.0% |
| ! | TAAGTCCCTAAACTTTGAAA+TGG | - | chr3.1:81347803-81347822 | MS.gene32916:intron | 30.0% |
| ! | TTTTGTATAAGCTATCTTGG+AGG | - | chr3.1:81347271-81347290 | MS.gene32916:CDS | 30.0% |
| !! | AAGTCCCTAAACTTTGAAAT+GGG | - | chr3.1:81347804-81347823 | MS.gene32916:intron | 30.0% |
| !! | GCTTCCATTTTTAATACCTA+CGG | + | chr3.1:81347774-81347793 | None:intergenic | 30.0% |
| !!! | ATTGACTTGAAATCGTTTTC+TGG | - | chr3.1:81348509-81348528 | MS.gene32916:CDS | 30.0% |
| AAAAAGAGAAGACCAATGGT+TGG | - | chr3.1:81346865-81346884 | MS.gene32916:CDS | 35.0% | |
| AACTGTTATGTTTCCCTTTC+AGG | - | chr3.1:81347934-81347953 | MS.gene32916:intron | 35.0% | |
| AATTGGAATCAATGGAAGGA+AGG | + | chr3.1:81346634-81346653 | None:intergenic | 35.0% | |
| ATTGGAATCAATGGAAGGAA+GGG | + | chr3.1:81346633-81346652 | None:intergenic | 35.0% | |
| CATGTGAACAATGTCATAAG+TGG | - | chr3.1:81347319-81347338 | MS.gene32916:intron | 35.0% | |
| CCAATTACTTCATTTCAGCA+AGG | + | chr3.1:81347895-81347914 | None:intergenic | 35.0% | |
| CCTTGCTGAAATGAAGTAAT+TGG | - | chr3.1:81347892-81347911 | MS.gene32916:intron | 35.0% | |
| CTACATTGGAATTTCTGAGT+GGG | + | chr3.1:81346578-81346597 | None:intergenic | 35.0% | |
| GCATTACATCAAACATGAGA+GGG | - | chr3.1:81346911-81346930 | MS.gene32916:CDS | 35.0% | |
| GCTACCGTAGGTATTAAAAA+TGG | - | chr3.1:81347767-81347786 | MS.gene32916:intron | 35.0% | |
| GTTGCCCATTTCAAAGTTTA+GGG | + | chr3.1:81347811-81347830 | None:intergenic | 35.0% | |
| TCAAGAGCAATGACATGAAT+AGG | + | chr3.1:81347676-81347695 | None:intergenic | 35.0% | |
| TCGGAGATATCAGATACATA+AGG | + | chr3.1:81346716-81346735 | None:intergenic | 35.0% | |
| TGACAAATACAAGCAGAACA+AGG | - | chr3.1:81348427-81348446 | MS.gene32916:CDS | 35.0% | |
| TTCTCAAGATAACCAACCAT+TGG | + | chr3.1:81346880-81346899 | None:intergenic | 35.0% | |
| ! | ATGAACCGGCTTATTCAAAT+TGG | + | chr3.1:81346651-81346670 | None:intergenic | 35.0% |
| ! | ATTGGTTGATTGGTTAGTTG+AGG | - | chr3.1:81346936-81346955 | MS.gene32916:CDS | 35.0% |
| ! | TCTGGAACAGTTAAAATGTG+TGG | - | chr3.1:81348527-81348546 | MS.gene32916:CDS | 35.0% |
| ! | TTTTTAATACCTACGGTAGC+AGG | + | chr3.1:81347767-81347786 | None:intergenic | 35.0% |
| !! | AAGTAATGATTCGGTATCTC+TGG | + | chr3.1:81348570-81348589 | None:intergenic | 35.0% |
| !! | GTGGGTTGATAATCAAAAAC+AGG | + | chr3.1:81346560-81346579 | None:intergenic | 35.0% |
| !!! | ATCACGTTTGGCATTGATTT+TGG | + | chr3.1:81346675-81346694 | None:intergenic | 35.0% |
| !!! | GCATTGATTTTGGTATGAAC+CGG | + | chr3.1:81346665-81346684 | None:intergenic | 35.0% |
| ATAGGATGTTTACCTCTTCC+AGG | + | chr3.1:81347658-81347677 | None:intergenic | 40.0% | |
| ATCAAACATGAGAGGGACAT+TGG | - | chr3.1:81346918-81346937 | MS.gene32916:CDS | 40.0% | |
| ATCCCAATAAACCTCCTGAA+AGG | + | chr3.1:81347951-81347970 | None:intergenic | 40.0% | |
| CCACTCAGAAATTCCAATGT+AGG | - | chr3.1:81346576-81346595 | MS.gene32916:intron | 40.0% | |
| CCTACATTGGAATTTCTGAG+TGG | + | chr3.1:81346579-81346598 | None:intergenic | 40.0% | |
| CTCCATTGTGCGAAGATAAT+CGG | + | chr3.1:81346735-81346754 | None:intergenic | 40.0% | |
| GATTGCTTGTGAGAATCGAA+AGG | - | chr3.1:81347968-81347987 | MS.gene32916:intron | 40.0% | |
| GCAGAAAAAGAGAAGACCAA+TGG | - | chr3.1:81346861-81346880 | MS.gene32916:CDS | 40.0% | |
| GGCATTACATCAAACATGAG+AGG | - | chr3.1:81346910-81346929 | MS.gene32916:CDS | 40.0% | |
| GGTTGCCCATTTCAAAGTTT+AGG | + | chr3.1:81347812-81347831 | None:intergenic | 40.0% | |
| TACTGATAACACGTATGACC+TGG | - | chr3.1:81347637-81347656 | MS.gene32916:intron | 40.0% | |
| TCCCAATAAACCTCCTGAAA+GGG | + | chr3.1:81347950-81347969 | None:intergenic | 40.0% | |
| TCCCTTTCAGGAGGTTTATT+GGG | - | chr3.1:81347946-81347965 | MS.gene32916:intron | 40.0% | |
| TGCTGACAGGTTCAATAGAT+AGG | + | chr3.1:81347026-81347045 | None:intergenic | 40.0% | |
| TGGCTGAATTCTTGAGGTTA+GGG | + | chr3.1:81346607-81346626 | None:intergenic | 40.0% | |
| TGTTATGTTTCCCTTTCAGG+AGG | - | chr3.1:81347937-81347956 | MS.gene32916:intron | 40.0% | |
| TTCCCTTTCAGGAGGTTTAT+TGG | - | chr3.1:81347945-81347964 | MS.gene32916:intron | 40.0% | |
| TTGTATATGTCAAGAAGGGC+AGG | - | chr3.1:81348386-81348405 | MS.gene32916:CDS | 40.0% | |
| ! | CTCTAAGGGCATGTTTGTAT+TGG | + | chr3.1:81347406-81347425 | None:intergenic | 40.0% |
| ! | GGTTAGGGTTTTTCCTACAT+TGG | + | chr3.1:81346592-81346611 | None:intergenic | 40.0% |
| ! | TTTGTCACGAACAGCTTTGA+AGG | + | chr3.1:81348414-81348433 | None:intergenic | 40.0% |
| !! | ACAAAGATCTAGGCCTTGTT+TGG | - | chr3.1:81347146-81347165 | MS.gene32916:intron | 40.0% |
| !! | CAAAGATCTAGGCCTTGTTT+GGG | - | chr3.1:81347147-81347166 | MS.gene32916:intron | 40.0% |
| !! | TCTTGCTGACTTAAGCTTGT+TGG | - | chr3.1:81348114-81348133 | MS.gene32916:CDS | 40.0% |
| !! | TGGTTGTTGATTGGTGTTGT+TGG | + | chr3.1:81346474-81346493 | None:intergenic | 40.0% |
| AAGCCTCATCACACTAAGTG+GGG | + | chr3.1:81347498-81347517 | None:intergenic | 45.0% | |
| AGATCTAAGCTTCAGCTGCT+TGG | - | chr3.1:81347045-81347064 | MS.gene32916:intron | 45.0% | |
| ATCAATGGAAGGAAGGGTTG+TGG | + | chr3.1:81346627-81346646 | None:intergenic | 45.0% | |
| CAAGCCTCATCACACTAAGT+GGG | + | chr3.1:81347499-81347518 | None:intergenic | 45.0% | |
| CGATTATCTTCGCACAATGG+AGG | - | chr3.1:81346733-81346752 | MS.gene32916:CDS | 45.0% | |
| CTCCGATTATCTTCGCACAA+TGG | - | chr3.1:81346730-81346749 | MS.gene32916:CDS | 45.0% | |
| CTTAGTGTGATGAGGCTTGA+TGG | - | chr3.1:81347500-81347519 | MS.gene32916:intron | 45.0% | |
| GACTTGTGGTGGTTGTTGAT+TGG | + | chr3.1:81346483-81346502 | None:intergenic | 45.0% | |
| GTAACCCAAGCATTCACACA+GGG | + | chr3.1:81348324-81348343 | None:intergenic | 45.0% | |
| GTGGCTGAATTCTTGAGGTT+AGG | + | chr3.1:81346608-81346627 | None:intergenic | 45.0% | |
| TAACACGTATGACCTGGAAG+AGG | - | chr3.1:81347643-81347662 | MS.gene32916:intron | 45.0% | |
| TCAACAACCACCACAAGTCA+TGG | - | chr3.1:81346484-81346503 | MS.gene32916:CDS | 45.0% | |
| TCAAGCCTCATCACACTAAG+TGG | + | chr3.1:81347500-81347519 | None:intergenic | 45.0% | |
| TCATGGTTCCCAAAAGACAG+AGG | - | chr3.1:81346501-81346520 | MS.gene32916:CDS | 45.0% | |
| TCCAGGCCGGATAATAAATC+TGG | + | chr3.1:81348197-81348216 | None:intergenic | 45.0% | |
| TGAAGCTTAGATCTGCTGAC+AGG | + | chr3.1:81347039-81347058 | None:intergenic | 45.0% | |
| TGAATGCTTGGGTTACACGT+CGG | - | chr3.1:81348328-81348347 | MS.gene32916:intron | 45.0% | |
| TGTAACCCAAGCATTCACAC+AGG | + | chr3.1:81348325-81348344 | None:intergenic | 45.0% | |
| TTTACCCAAGGATGTACTCC+AGG | + | chr3.1:81348214-81348233 | None:intergenic | 45.0% | |
| ! | TGAGAGGGACATTGGTTGAT+TGG | - | chr3.1:81346926-81346945 | MS.gene32916:CDS | 45.0% |
| !! | CTTTTGGGAACCATGACTTG+TGG | + | chr3.1:81346497-81346516 | None:intergenic | 45.0% |
| !! | GTTGTTGGCTCTCTTCTTTG+AGG | + | chr3.1:81346459-81346478 | None:intergenic | 45.0% |
| !!! | TTATAATTATTTCTTATTAT+TGG | - | chr3.1:81348480-81348499 | MS.gene32916:CDS | 5.0% |
| !!! | TTATTTTATTTACAATTTTT+AGG | - | chr3.1:81346805-81346824 | MS.gene32916:intron | 5.0% |
| ACTAAGTGGGGTCTGCTACA+TGG | + | chr3.1:81347486-81347505 | None:intergenic | 50.0% | |
| AGAAATCCACTGCCTTCGGT+GGG | + | chr3.1:81347610-81347629 | None:intergenic | 50.0% | |
| AGACCCCACTTAGTGTGATG+AGG | - | chr3.1:81347492-81347511 | MS.gene32916:intron | 50.0% | |
| GCCAGATTTATTATCCGGCC+TGG | - | chr3.1:81348193-81348212 | MS.gene32916:CDS | 50.0% | |
| GGGTTGTGGCTGAATTCTTG+AGG | + | chr3.1:81346613-81346632 | None:intergenic | 50.0% | |
| TGAAGAAATCACCCCACCGA+AGG | - | chr3.1:81347595-81347614 | MS.gene32916:intron | 50.0% | |
| TGGCAGAAATCCACTGCCTT+CGG | + | chr3.1:81347614-81347633 | None:intergenic | 50.0% | |
| TGTTGTCTTCCTGCTACCGT+AGG | - | chr3.1:81347755-81347774 | MS.gene32916:intron | 50.0% | |
| TTCGGTATCTCTGGCGATGA+AGG | + | chr3.1:81348561-81348580 | None:intergenic | 50.0% | |
| ! | ACTGACAGATGAAGCGTCTC+TGG | + | chr3.1:81346985-81347004 | None:intergenic | 50.0% |
| ! | TTGGGAACCATGACTTGTGG+TGG | + | chr3.1:81346494-81346513 | None:intergenic | 50.0% |
| ACCGATGCAGCGACAACAGA+TGG | + | chr3.1:81348163-81348182 | None:intergenic | 55.0% | |
| CAGAAATCCACTGCCTTCGG+TGG | + | chr3.1:81347611-81347630 | None:intergenic | 55.0% | |
| CCCAAGCATTCACACAGGGA+CGG | + | chr3.1:81348320-81348339 | None:intergenic | 55.0% | |
| CCGTCCCTGTGTGAATGCTT+GGG | - | chr3.1:81348317-81348336 | MS.gene32916:intron | 55.0% | |
| GAAATCCACTGCCTTCGGTG+GGG | + | chr3.1:81347609-81347628 | None:intergenic | 55.0% | |
| GCCATCTGTTGTCGCTGCAT+CGG | - | chr3.1:81348159-81348178 | MS.gene32916:CDS | 55.0% | |
| GTATCTCTGGCGATGAAGGC+AGG | + | chr3.1:81348557-81348576 | None:intergenic | 55.0% | |
| TCCGTCCCTGTGTGAATGCT+TGG | - | chr3.1:81348316-81348335 | MS.gene32916:intron | 55.0% | |
| AATCACCCCACCGAAGGCAG+TGG | - | chr3.1:81347601-81347620 | MS.gene32916:intron | 60.0% | |
| CACAGGGACGGAGTCTGCAA+TGG | + | chr3.1:81348308-81348327 | None:intergenic | 60.0% | |
| CCCAAGGATGTACTCCAGGC+CGG | + | chr3.1:81348210-81348229 | None:intergenic | 60.0% | |
| CCGGCCTGGAGTACATCCTT+GGG | - | chr3.1:81348207-81348226 | MS.gene32916:intron | 60.0% | |
| TCCGGCCTGGAGTACATCCT+TGG | - | chr3.1:81348206-81348225 | MS.gene32916:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 81346443 | 81348641 | 81346443 | ID=MS.gene32916 |
| chr3.1 | mRNA | 81346443 | 81348641 | 81346443 | ID=MS.gene32916.t1;Parent=MS.gene32916 |
| chr3.1 | exon | 81348330 | 81348641 | 81348330 | ID=MS.gene32916.t1.exon1;Parent=MS.gene32916.t1 |
| chr3.1 | CDS | 81348330 | 81348641 | 81348330 | ID=cds.MS.gene32916.t1;Parent=MS.gene32916.t1 |
| chr3.1 | exon | 81347993 | 81348225 | 81347993 | ID=MS.gene32916.t1.exon2;Parent=MS.gene32916.t1 |
| chr3.1 | CDS | 81347993 | 81348225 | 81347993 | ID=cds.MS.gene32916.t1;Parent=MS.gene32916.t1 |
| chr3.1 | exon | 81347420 | 81347495 | 81347420 | ID=MS.gene32916.t1.exon3;Parent=MS.gene32916.t1 |
| chr3.1 | CDS | 81347420 | 81347495 | 81347420 | ID=cds.MS.gene32916.t1;Parent=MS.gene32916.t1 |
| chr3.1 | exon | 81347231 | 81347307 | 81347231 | ID=MS.gene32916.t1.exon4;Parent=MS.gene32916.t1 |
| chr3.1 | CDS | 81347231 | 81347307 | 81347231 | ID=cds.MS.gene32916.t1;Parent=MS.gene32916.t1 |
| chr3.1 | exon | 81347095 | 81347128 | 81347095 | ID=MS.gene32916.t1.exon5;Parent=MS.gene32916.t1 |
| chr3.1 | CDS | 81347095 | 81347128 | 81347095 | ID=cds.MS.gene32916.t1;Parent=MS.gene32916.t1 |
| chr3.1 | exon | 81346856 | 81347005 | 81346856 | ID=MS.gene32916.t1.exon6;Parent=MS.gene32916.t1 |
| chr3.1 | CDS | 81346856 | 81347005 | 81346856 | ID=cds.MS.gene32916.t1;Parent=MS.gene32916.t1 |
| chr3.1 | exon | 81346636 | 81346770 | 81346636 | ID=MS.gene32916.t1.exon7;Parent=MS.gene32916.t1 |
| chr3.1 | CDS | 81346636 | 81346770 | 81346636 | ID=cds.MS.gene32916.t1;Parent=MS.gene32916.t1 |
| chr3.1 | exon | 81346443 | 81346547 | 81346443 | ID=MS.gene32916.t1.exon8;Parent=MS.gene32916.t1 |
| chr3.1 | CDS | 81346443 | 81346547 | 81346443 | ID=cds.MS.gene32916.t1;Parent=MS.gene32916.t1 |
| Gene Sequence |
| Protein sequence |
