AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene06093

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene06093.t1 MTR_8g099640 98.044 869 17 0 1 869 1 869 0.0 1779
MS.gene06093.t1 MTR_3g060290 86.585 82 8 3 165 245 17 96 1.04e-31 121
MS.gene06093.t1 MTR_3g463490 30.855 269 166 7 493 754 80 335 1.44e-27 118
MS.gene06093.t1 MTR_3g463490 30.855 269 166 7 493 754 80 335 3.27e-27 117
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene06093.t1 AT4G14700 66.086 746 209 10 130 869 81 788 0.0 973
MS.gene06093.t1 AT4G12620 70.606 677 180 6 197 869 131 792 0.0 961
MS.gene06093.t1 AT1G07270 28.972 321 204 10 490 799 110 417 2.23e-31 129
MS.gene06093.t1 AT1G07270 28.972 321 204 10 490 799 110 417 3.70e-31 129
MS.gene06093.t1 AT2G29680 27.469 324 207 11 489 799 112 420 2.72e-26 114
MS.gene06093.t1 AT2G29680 25.581 344 197 12 489 788 112 440 6.85e-21 98.2

Find 215 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCGATGAATCTGGCGATTT+TGG 0.133439 4.2:-4187981 None:intergenic
GTTAGTTACCGACTGGTTTC+TGG 0.202474 4.2:-4191526 None:intergenic
TGAAGCTGCTGATAATTATA+TGG 0.208304 4.2:-4191944 None:intergenic
CTGCACAAGGGACGGTTCTT+TGG 0.215974 4.2:+4190712 MS.gene06093:CDS
CGCCCAACAGAAGTCTCTTC+TGG 0.247491 4.2:-4188737 None:intergenic
TCATCGGTTTGCATCATTAA+TGG 0.257487 4.2:-4195026 None:intergenic
CTCTATATTCACGGTGTTCC+GGG 0.266627 4.2:+4191017 MS.gene06093:CDS
TGGCAGCTGAAGAAGTAATA+AGG 0.285779 4.2:-4195006 None:intergenic
CCTCTATATTCACGGTGTTC+CGG 0.288739 4.2:+4191016 MS.gene06093:CDS
GTCATATATGAAGCATTAAA+TGG 0.289669 4.2:+4191378 MS.gene06093:CDS
CTTCAGGTTGCAGCTATATC+AGG 0.290038 4.2:+4192148 MS.gene06093:intron
TTTACAGATTTCGATGAATC+TGG 0.295754 4.2:-4187990 None:intergenic
AACCTACATTCCTCCACTTC+TGG 0.299054 4.2:-4188308 None:intergenic
CCGATTCTTCTCCCTCTTCT+CGG 0.303926 4.2:-4187798 None:intergenic
CTAGCTTTGGCATACTCCTT+AGG 0.308499 4.2:-4190198 None:intergenic
ATCTCCCTCATTGCTAGCTT+TGG 0.313313 4.2:-4190211 None:intergenic
TTCTTACCCTTCGGCGGCTT+TGG 0.317699 4.2:-4188488 None:intergenic
GGAGATCAATGGTCTGAAAT+TGG 0.328776 4.2:+4191245 MS.gene06093:CDS
GGGAGATTGAAGAAGCTTCT+TGG 0.333604 4.2:-4187637 None:intergenic
AAAGAATTCCAGAGCATATA+AGG 0.339598 4.2:+4190755 MS.gene06093:CDS
ATACCTGCAGCAACATTATC+AGG 0.340644 4.2:-4192250 None:intergenic
TTTGTAGAAGGGAAGAAAAC+TGG 0.345764 4.2:+4191447 MS.gene06093:CDS
CTAAATGAGAGGTTTGTAGA+AGG 0.346073 4.2:+4191435 MS.gene06093:CDS
ATTGAGTGTGATAGTTGTTT+AGG 0.355173 4.2:+4188359 MS.gene06093:CDS
TGAGATTCGGCAACGAGATT+GGG 0.367135 4.2:-4187759 None:intergenic
TTGATCTCCACGAAACAGTA+TGG 0.382365 4.2:-4191231 None:intergenic
CCACATATCCAATCCCCTTC+AGG 0.385042 4.2:-4188425 None:intergenic
GCTGAATTGAAGAAGAGAAA+TGG 0.386079 4.2:+4188041 MS.gene06093:CDS
TAGCTTTGGCATACTCCTTA+GGG 0.393164 4.2:-4190197 None:intergenic
TTTCAACTCACTTGAATATG+AGG 0.394384 4.2:-4193624 None:intergenic
TTGAGATTCGGCAACGAGAT+TGG 0.395152 4.2:-4187760 None:intergenic
TATTTCTGACAATCAATGTC+TGG 0.396034 4.2:+4190986 MS.gene06093:CDS
ATAAGTCTTACCCAACTTGC+AGG 0.400447 4.2:-4194679 None:intergenic
AGAGTGACGGTGACGAAGAC+TGG 0.402154 4.2:+4190489 MS.gene06093:CDS
AGGAGATGCACGCCGTGCTT+TGG 0.412726 4.2:+4192168 MS.gene06093:CDS
GGAGATTGAAGAAGCTTCTT+GGG 0.412877 4.2:-4187636 None:intergenic
CTAACAAGCTTCTTACCCTT+CGG 0.416592 4.2:-4188497 None:intergenic
GCTATATCGGACCAACGATT+TGG 0.417826 4.2:+4188784 MS.gene06093:CDS
TGAGAATTGTGAACGGAGGT+TGG 0.424141 4.2:-4187670 None:intergenic
AAAACGGTTGTTACAAGAGC+TGG 0.428477 4.2:+4188158 MS.gene06093:CDS
GAAGCTGCTGATAATTATAT+GGG 0.430667 4.2:-4191943 None:intergenic
ATTTGAGCAGAGAGCTATAT+CGG 0.433378 4.2:+4188771 MS.gene06093:CDS
TTCTTTGGACTTCAGAAGAT+AGG 0.436400 4.2:+4190727 MS.gene06093:CDS
ATGATATCCTCCTGCAAGTT+GGG 0.437262 4.2:+4194669 MS.gene06093:CDS
AGCAATGGTGAAGTGTTTCC+TGG 0.439086 4.2:+4194644 MS.gene06093:CDS
GAGCTTTACAAAACAGGAAT+GGG 0.441059 4.2:+4194196 MS.gene06093:CDS
CGGAGCGAACGAAACCTTAA+GGG 0.442571 4.2:-4188075 None:intergenic
TCCGACGTCGCCGGATCAAT+CGG 0.442884 4.2:+4188094 MS.gene06093:CDS
GACGGCGGTGAATTTGAAGT+TGG 0.443655 4.2:+4188233 MS.gene06093:CDS
GGGCGTTGCCTCTATATTCA+CGG 0.455755 4.2:+4191008 MS.gene06093:CDS
GGCTTCAATCAAGTGATTTG+TGG 0.457739 4.2:+4188534 MS.gene06093:CDS
CATTCCAAGCTAATTGTGAT+AGG 0.457837 4.2:+4191672 MS.gene06093:CDS
ATCATTTCGAGTCGCCTCAA+TGG 0.464299 4.2:+4191971 MS.gene06093:CDS
ACGGCGGTGAATTTGAAGTT+GGG 0.465132 4.2:+4188234 MS.gene06093:CDS
CTTACCTATCACAATTAGCT+TGG 0.465486 4.2:-4191676 None:intergenic
AAGATGTTCCTGAAGGGGAT+TGG 0.466368 4.2:+4188417 MS.gene06093:CDS
GGCAGCTGAAGAAGTAATAA+GGG 0.468817 4.2:-4195005 None:intergenic
GTGCACGAGCTTTACAAAAC+AGG 0.469313 4.2:+4194190 MS.gene06093:CDS
TGATTGTTGAATTTGAGATT+CGG 0.470559 4.2:-4187772 None:intergenic
CATCAAACCATACTGTTTCG+TGG 0.472974 4.2:+4191224 MS.gene06093:CDS
TCGGAGCGAACGAAACCTTA+AGG 0.473564 4.2:-4188076 None:intergenic
TTTCTACAAGGTAAAGACTT+AGG 0.474900 4.2:-4190835 None:intergenic
TTGTGAGACCTTATATGCTC+TGG 0.474919 4.2:-4190763 None:intergenic
TTCTTAGTTTCCGATTGATC+CGG 0.476110 4.2:-4188104 None:intergenic
GCCCAACAGAAGTCTCTTCT+GGG 0.476671 4.2:-4188736 None:intergenic
ATACGAGGAAGTAACTTCTC+TGG 0.476939 4.2:-4191884 None:intergenic
TTCGTTCGCTCCGACGTCGC+CGG 0.480213 4.2:+4188085 MS.gene06093:CDS
GCTTCAATCAAGTGATTTGT+GGG 0.480427 4.2:+4188535 MS.gene06093:CDS
CCTGAAGGGGATTGGATATG+TGG 0.480446 4.2:+4188425 MS.gene06093:CDS
TGGGAAGGGAGATTCCCTTA+AGG 0.483741 4.2:+4188061 MS.gene06093:CDS
CTGAATTGAAGAAGAGAAAT+GGG 0.485254 4.2:+4188042 MS.gene06093:CDS
GAGTGTGATAGTTGTTTAGG+TGG 0.490466 4.2:+4188362 MS.gene06093:CDS
TCATGTCTCTGCGGCAGCAA+TGG 0.493304 4.2:+4194629 MS.gene06093:CDS
TGGTCTGAAATTGGCTTCAC+CGG 0.494433 4.2:+4191254 MS.gene06093:CDS
TTCTGTACAATATTCTTGAC+TGG 0.495175 4.2:+4191637 MS.gene06093:CDS
GCAGCTACTGGTGTCGGGTA+CGG 0.495197 4.2:+4188702 MS.gene06093:CDS
TAAATGAGAGGTTTGTAGAA+GGG 0.497183 4.2:+4191436 MS.gene06093:CDS
ATTCACGGTGTTCCGGGCAC+TGG 0.498438 4.2:+4191023 MS.gene06093:CDS
AACAATGCAGTTGGCAATGA+CGG 0.500318 4.2:+4194604 MS.gene06093:intron
GTGGCACAGTTTCAAACGCT+TGG 0.500569 4.2:+4190269 MS.gene06093:CDS
GTTACAAGAGCTGGGAGAAA+AGG 0.500757 4.2:+4188167 MS.gene06093:CDS
AAAGCCTCTGGATGCCCATT+CGG 0.503391 4.2:-4191913 None:intergenic
GGATGGCAGCTACTGGTGTC+GGG 0.503839 4.2:+4188697 MS.gene06093:CDS
TTCACCTTGTAAATATTCTC+CGG 0.504027 4.2:-4191273 None:intergenic
CCGAGAAGAGGGAGAAGAAT+CGG 0.504286 4.2:+4187798 MS.gene06093:CDS
GCAGCTAACTGATGGCTTGT+CGG 0.504655 4.2:-4190596 None:intergenic
TGAGGTCAAGGTTGAGAAAA+CGG 0.504688 4.2:+4188142 MS.gene06093:CDS
ATCTGTGAGGGTCGTAAGAT+GGG 0.506846 4.2:+4188449 MS.gene06093:CDS
CCTCGTATATCAAGCCGAAT+GGG 0.511380 4.2:+4191899 MS.gene06093:CDS
GATAGTACCTTGCCAGTGCC+CGG 0.512164 4.2:-4191035 None:intergenic
ATCCCAGAAGAGACTTCTGT+TGG 0.512492 4.2:+4188734 MS.gene06093:CDS
TCAAGACGAGATTTCGCAGC+TGG 0.512532 4.2:-4187834 None:intergenic
CGGAAACTAAGAAGAGAAAG+AGG 0.512657 4.2:+4188114 MS.gene06093:CDS
GATCTGTGAGGGTCGTAAGA+TGG 0.515072 4.2:+4188448 MS.gene06093:CDS
TTGAAGTCAGAAGTTGATGC+AGG 0.515204 4.2:+4191198 MS.gene06093:CDS
ATTTCTGACAATCAATGTCT+GGG 0.516051 4.2:+4190987 MS.gene06093:CDS
AGCTTGGAATGTGGCTTGGT+TGG 0.517458 4.2:-4191660 None:intergenic
CAAATATATCAATTCCATTG+AGG 0.517649 4.2:-4191985 None:intergenic
TGGAGCAAATGCAATTTCAT+CGG 0.518476 4.2:-4187961 None:intergenic
TACTGTTTCGTGGAGATCAA+TGG 0.518500 4.2:+4191234 MS.gene06093:CDS
GATCCTGATAATGTTGCTGC+AGG 0.521500 4.2:+4192247 MS.gene06093:CDS
TACAAGAAAGTTGTTTATGA+CGG 0.522606 4.2:+4188215 MS.gene06093:CDS
GCATTAAATGGACACAGGGT+TGG 0.523201 4.2:+4191390 MS.gene06093:CDS
AAAGAGGAAGAATGAGGTCA+AGG 0.524216 4.2:+4188130 MS.gene06093:CDS
GGTAAGGAATGAGGAGTGAG+AGG 0.524682 4.2:-4187693 None:intergenic
TGGGAGAAAAGGTGGAAAGA+TGG 0.525437 4.2:+4188178 MS.gene06093:CDS
CAAACCTCTCATTTAGCAAA+CGG 0.525477 4.2:-4191428 None:intergenic
ATGGGGCAAAGCAAAGCCTC+TGG 0.529383 4.2:-4191925 None:intergenic
AGCGTTTGAAACTGTGCCAC+TGG 0.529464 4.2:-4190266 None:intergenic
TCAACATACTTATTTCTACA+AGG 0.530757 4.2:-4190847 None:intergenic
TTGAAGAAGAGAAATGGGAA+GGG 0.532289 4.2:+4188047 MS.gene06093:CDS
GCTCGTGCACCATAGCTGTC+AGG 0.532743 4.2:-4194177 None:intergenic
CGTGGATCTACGTGTAGGTA+AGG 0.533392 4.2:-4187709 None:intergenic
CTCGATGTTGGCCAAATCGT+TGG 0.533909 4.2:-4188795 None:intergenic
TGGATGGCAGCTACTGGTGT+CGG 0.533932 4.2:+4188696 MS.gene06093:CDS
ATCTGTAAAGAAGAGGAAGC+AGG 0.534383 4.2:+4188004 MS.gene06093:CDS
TCACTTGAATATGAGGTGCT+TGG 0.534848 4.2:-4193617 None:intergenic
CGAGCTTTACAAAACAGGAA+TGG 0.537464 4.2:+4194195 MS.gene06093:CDS
AATTAGCTTGGAATGTGGCT+TGG 0.540690 4.2:-4191664 None:intergenic
CGGTGTTCCGGGCACTGGCA+AGG 0.541081 4.2:+4191028 MS.gene06093:CDS
ATCCAGAAGTGGAGGAATGT+AGG 0.542570 4.2:+4188306 MS.gene06093:CDS
GAGATTCGGCAACGAGATTG+GGG 0.543738 4.2:-4187758 None:intergenic
TAAATGGACACAGGGTTGGA+TGG 0.543748 4.2:+4191394 MS.gene06093:CDS
GTGAAGCAGGTGCCAAGCAC+AGG 0.543759 4.2:+4194939 MS.gene06093:CDS
AGTGTGATAGTTGTTTAGGT+GGG 0.547500 4.2:+4188363 MS.gene06093:CDS
CATGATATCCTCCTGCAAGT+TGG 0.553301 4.2:+4194668 MS.gene06093:CDS
CCGGAACACCGTGAATATAG+AGG 0.553353 4.2:-4191016 None:intergenic
CATCGAAATCTGTAAAGAAG+AGG 0.553381 4.2:+4187997 MS.gene06093:CDS
TCCTCGTATATCAAGCCGAA+TGG 0.555101 4.2:+4191898 MS.gene06093:CDS
TTCTTGGGAGTTGAAGCCAT+TGG 0.556223 4.2:-4187621 None:intergenic
GGGATGATGTTTATGTGAAG+AGG 0.559397 4.2:+4188255 MS.gene06093:CDS
TGCAGGAGGATATCATGTCC+AGG 0.562882 4.2:-4194662 None:intergenic
GAAGAGAAAGAGGAAGAATG+AGG 0.565359 4.2:+4188124 MS.gene06093:CDS
AACAATCAACTCCGAGAAGA+GGG 0.566074 4.2:+4187787 MS.gene06093:CDS
AGCTCTCTGCTCAAATTATG+TGG 0.566453 4.2:-4188764 None:intergenic
TTCATACTGCAGCTAACTGA+TGG 0.566543 4.2:-4190604 None:intergenic
ATTGAAGAAGAGAAATGGGA+AGG 0.568468 4.2:+4188046 MS.gene06093:CDS
TCAATCCATCACCTCGATGT+TGG 0.568619 4.2:-4188807 None:intergenic
TTGGATATGTGGGATCTGTG+AGG 0.570339 4.2:+4188436 MS.gene06093:CDS
CTCTCCGTTTGCTAAATGAG+AGG 0.575524 4.2:+4191424 MS.gene06093:CDS
TGTGGGCTGCTCGCATTGAG+AGG 0.577481 4.2:+4188552 MS.gene06093:CDS
AGGGAAGAAAACTGGAGAAG+AGG 0.577588 4.2:+4191455 MS.gene06093:CDS
TCCCAGAAGAGACTTCTGTT+GGG 0.577921 4.2:+4188735 MS.gene06093:CDS
AAGAAGTGGATGGCAGCTAC+TGG 0.578137 4.2:+4188690 MS.gene06093:CDS
GGGCTTTGAGAAATAACCTC+GGG 0.578651 4.2:-4194985 None:intergenic
CGTTGAAAGATGTTCCTGAA+GGG 0.581179 4.2:+4188411 MS.gene06093:CDS
TCCGATTGATCCGGCGACGT+CGG 0.582582 4.2:-4188095 None:intergenic
TATGCCAAAGCTAGCAATGA+GGG 0.585809 4.2:+4190207 MS.gene06093:CDS
TGAGAGGTGAGAATTGTGAA+CGG 0.585848 4.2:-4187677 None:intergenic
TTGCTATGTCATGATCCCTA+AGG 0.591823 4.2:+4190182 MS.gene06093:CDS
TTGTTAGAACAATGCGTCAG+AGG 0.591921 4.2:+4188513 MS.gene06093:CDS
CCGTTGAAAGATGTTCCTGA+AGG 0.592540 4.2:+4188410 MS.gene06093:CDS
CTGAAGGGGATTGGATATGT+GGG 0.593658 4.2:+4188426 MS.gene06093:CDS
ACACAGGGTTGGATGGAAAG+AGG 0.594099 4.2:+4191401 MS.gene06093:CDS
AGGAAAGGGACTTGTTTGCA+TGG 0.594820 4.2:+4193563 MS.gene06093:intron
ACAAGCTTCTTACCCTTCGG+CGG 0.596059 4.2:-4188494 None:intergenic
CAACAATCAACTCCGAGAAG+AGG 0.596926 4.2:+4187786 MS.gene06093:CDS
TAGCAGTATATGGAAAGAAG+TGG 0.597595 4.2:+4188676 MS.gene06093:intron
AAACGGTTGTTACAAGAGCT+GGG 0.597984 4.2:+4188159 MS.gene06093:CDS
GTTGAAAGATGTTCCTGAAG+GGG 0.598838 4.2:+4188412 MS.gene06093:CDS
ATGAAGCATTAAATGGACAC+AGG 0.600808 4.2:+4191385 MS.gene06093:CDS
TACAGAATCTGCACAAGGGA+CGG 0.603574 4.2:+4190704 MS.gene06093:intron
GCTGTAAGTTGGGTGAGTGT+AGG 0.604738 4.2:+4194906 MS.gene06093:intron
GACTAATCTTGAAAGAGCAA+AGG 0.606188 4.2:+4190789 MS.gene06093:CDS
GTCTGCATATTTCCAAAGCA+CGG 0.606454 4.2:-4192180 None:intergenic
TGGATATGTGGGATCTGTGA+GGG 0.607873 4.2:+4188437 MS.gene06093:CDS
TTCACCGGAGAATATTTACA+AGG 0.608665 4.2:+4191269 MS.gene06093:CDS
TTCAGGGATAGCAAATACAA+TGG 0.610395 4.2:+4191856 MS.gene06093:intron
AATTTCTGAAGAAGAGAAGT+CGG 0.611112 4.2:+4187928 MS.gene06093:CDS
CGGCGGTGAATTTGAAGTTG+GGG 0.617237 4.2:+4188235 MS.gene06093:CDS
CCTTCAGGAACATCTTTCAA+CGG 0.617438 4.2:-4188410 None:intergenic
AGAAGATGCTACTTCTGATG+AGG 0.621654 4.2:+4188280 MS.gene06093:CDS
ACAAGAGCTGGGAGAAAAGG+TGG 0.622286 4.2:+4188170 MS.gene06093:CDS
TCATGATTTCGTCCCCAGAA+CGG 0.622364 4.2:-4188337 None:intergenic
TCGTCAATGAGCAAAATACA+TGG 0.624876 4.2:-4191486 None:intergenic
TGAGGAAGATCCAGAAGTGG+AGG 0.628093 4.2:+4188298 MS.gene06093:CDS
GTATGCCAAAGCTAGCAATG+AGG 0.629062 4.2:+4190206 MS.gene06093:CDS
CTACGTGTAGGTAAGGAATG+AGG 0.635826 4.2:-4187702 None:intergenic
CAATGAGCAAAATACATGGT+CGG 0.635961 4.2:-4191482 None:intergenic
CCCATTCGGCTTGATATACG+AGG 0.638148 4.2:-4191899 None:intergenic
TGAAGCATTAAATGGACACA+GGG 0.640411 4.2:+4191386 MS.gene06093:CDS
AGTATCGGAGTTCAAGCGCG+TGG 0.641574 4.2:-4187727 None:intergenic
AAGCGCGTGGATCTACGTGT+AGG 0.642274 4.2:-4187714 None:intergenic
GCTACTGGTGTCGGGTACGG+TGG 0.642777 4.2:+4188705 MS.gene06093:CDS
GCTAACTGATGGCTTGTCGG+TGG 0.645325 4.2:-4190593 None:intergenic
TGATGAGGAAGATCCAGAAG+TGG 0.647235 4.2:+4188295 MS.gene06093:CDS
AGGGCTTTGAGAAATAACCT+CGG 0.647966 4.2:-4194986 None:intergenic
ATCACAATTAGCTTGGAATG+TGG 0.648587 4.2:-4191669 None:intergenic
AGTATATGGAAAGAAGTGGA+TGG 0.649668 4.2:+4188680 MS.gene06093:intron
AGATTCGGCAACGAGATTGG+GGG 0.651554 4.2:-4187757 None:intergenic
TCATGACATAGCAATGTCGA+AGG 0.658256 4.2:-4190173 None:intergenic
AAGAAAGTTGTTTATGACGG+CGG 0.658330 4.2:+4188218 MS.gene06093:CDS
GAGATAACGACATTCATCAA+AGG 0.658884 4.2:+4190960 MS.gene06093:CDS
TGAGTGTACTCTCAGTGATG+AGG 0.663135 4.2:+4191172 MS.gene06093:CDS
TCTTGTAACCAGAAACCAGT+CGG 0.664804 4.2:+4191518 MS.gene06093:CDS
CGAAGACTGGAACATCGACA+AGG 0.665993 4.2:+4190502 MS.gene06093:CDS
AAAAGGTGGAAAGATGGCGA+AGG 0.670267 4.2:+4188184 MS.gene06093:CDS
AAGCTGCTGATAATTATATG+GGG 0.670932 4.2:-4191942 None:intergenic
GAGGTGAGAATTGTGAACGG+AGG 0.673704 4.2:-4187674 None:intergenic
TGACATTGATGATGAAAGAG+AGG 0.674539 4.2:+4190293 MS.gene06093:CDS
AGTCTTACCCAACTTGCAGG+AGG 0.676386 4.2:-4194676 None:intergenic
TCAGGAACATCTTTCAACGG+TGG 0.680289 4.2:-4188407 None:intergenic
GCTTTACAAAACAGGAATGG+GGG 0.682677 4.2:+4194198 MS.gene06093:CDS
AGCTTTACAAAACAGGAATG+GGG 0.683635 4.2:+4194197 MS.gene06093:CDS
AATGATACAGAATCTGCACA+AGG 0.686949 4.2:+4190699 MS.gene06093:intron
GTAAAGAAGAGGAAGCAGGA+AGG 0.690620 4.2:+4188008 MS.gene06093:CDS
AGTTGCAGCTCAATTTCCCG+AGG 0.692840 4.2:+4194969 MS.gene06093:CDS
TGAAGATGTTGAATATGATG+AGG 0.693036 4.2:+4190544 MS.gene06093:CDS
CAACGATTTGGCCAACATCG+AGG 0.693338 4.2:+4188796 MS.gene06093:CDS
TTTCTGACAATCAATGTCTG+GGG 0.698842 4.2:+4190988 MS.gene06093:CDS
CAAGCCGAATGGGCATCCAG+AGG 0.706358 4.2:+4191909 MS.gene06093:CDS
AATATGAATATGACATCCAG+TGG 0.707312 4.2:+4190250 MS.gene06093:CDS
ATGACGGTTTCATGTCTCTG+CGG 0.719836 4.2:+4194620 MS.gene06093:CDS
ATGATACAGAATCTGCACAA+GGG 0.728041 4.2:+4190700 MS.gene06093:intron
TAGAATTCTGATGAGAGTGA+CGG 0.741239 4.2:+4190476 MS.gene06093:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr4.2 gene 4187624 4195048 4187624 ID=MS.gene06093
chr4.2 mRNA 4187624 4195048 4187624 ID=MS.gene06093.t1;Parent=MS.gene06093
chr4.2 exon 4187624 4188573 4187624 ID=MS.gene06093.t1.exon1;Parent=MS.gene06093.t1
chr4.2 CDS 4187624 4188573 4187624 ID=cds.MS.gene06093.t1;Parent=MS.gene06093.t1
chr4.2 exon 4188682 4188817 4188682 ID=MS.gene06093.t1.exon2;Parent=MS.gene06093.t1
chr4.2 CDS 4188682 4188817 4188682 ID=cds.MS.gene06093.t1;Parent=MS.gene06093.t1
chr4.2 exon 4190162 4190314 4190162 ID=MS.gene06093.t1.exon3;Parent=MS.gene06093.t1
chr4.2 CDS 4190162 4190314 4190162 ID=cds.MS.gene06093.t1;Parent=MS.gene06093.t1
chr4.2 exon 4190479 4190619 4190479 ID=MS.gene06093.t1.exon4;Parent=MS.gene06093.t1
chr4.2 CDS 4190479 4190619 4190479 ID=cds.MS.gene06093.t1;Parent=MS.gene06093.t1
chr4.2 exon 4190709 4190860 4190709 ID=MS.gene06093.t1.exon5;Parent=MS.gene06093.t1
chr4.2 CDS 4190709 4190860 4190709 ID=cds.MS.gene06093.t1;Parent=MS.gene06093.t1
chr4.2 exon 4190950 4191049 4190950 ID=MS.gene06093.t1.exon6;Parent=MS.gene06093.t1
chr4.2 CDS 4190950 4191049 4190950 ID=cds.MS.gene06093.t1;Parent=MS.gene06093.t1
chr4.2 exon 4191168 4191290 4191168 ID=MS.gene06093.t1.exon7;Parent=MS.gene06093.t1
chr4.2 CDS 4191168 4191290 4191168 ID=cds.MS.gene06093.t1;Parent=MS.gene06093.t1
chr4.2 exon 4191378 4191539 4191378 ID=MS.gene06093.t1.exon8;Parent=MS.gene06093.t1
chr4.2 CDS 4191378 4191539 4191378 ID=cds.MS.gene06093.t1;Parent=MS.gene06093.t1
chr4.2 exon 4191636 4191693 4191636 ID=MS.gene06093.t1.exon9;Parent=MS.gene06093.t1
chr4.2 CDS 4191636 4191693 4191636 ID=cds.MS.gene06093.t1;Parent=MS.gene06093.t1
chr4.2 exon 4191861 4192051 4191861 ID=MS.gene06093.t1.exon10;Parent=MS.gene06093.t1
chr4.2 CDS 4191861 4192051 4191861 ID=cds.MS.gene06093.t1;Parent=MS.gene06093.t1
chr4.2 exon 4192154 4192268 4192154 ID=MS.gene06093.t1.exon11;Parent=MS.gene06093.t1
chr4.2 CDS 4192154 4192268 4192154 ID=cds.MS.gene06093.t1;Parent=MS.gene06093.t1
chr4.2 exon 4193565 4193635 4193565 ID=MS.gene06093.t1.exon12;Parent=MS.gene06093.t1
chr4.2 CDS 4193565 4193635 4193565 ID=cds.MS.gene06093.t1;Parent=MS.gene06093.t1
chr4.2 exon 4194142 4194237 4194142 ID=MS.gene06093.t1.exon13;Parent=MS.gene06093.t1
chr4.2 CDS 4194142 4194237 4194142 ID=cds.MS.gene06093.t1;Parent=MS.gene06093.t1
chr4.2 exon 4194614 4194690 4194614 ID=MS.gene06093.t1.exon14;Parent=MS.gene06093.t1
chr4.2 CDS 4194614 4194690 4194614 ID=cds.MS.gene06093.t1;Parent=MS.gene06093.t1
chr4.2 exon 4194907 4195048 4194907 ID=MS.gene06093.t1.exon15;Parent=MS.gene06093.t1
chr4.2 CDS 4194907 4195048 4194907 ID=cds.MS.gene06093.t1;Parent=MS.gene06093.t1
Gene Sequence

>MS.gene06093

ATGGCTTCAACTCCCAAGAAGCTTCTTCAATCTCCCTCCTCAAAATCCAACCTCCGTTCACAATTCTCACCTCTCACTCCTCATTCCTTACCTACACGTAGATCCACGCGCTTGAACTCCGATACTCCCATAACCCCCAATCTCGTTGCCGAATCTCAAATTCAACAATCAACTCCGAGAAGAGGGAGAAGAATCGGTGAGAATTCTGTTCCAGCTGCGAAATCTCGTCTTGATTTCACTCGAAAAGATAAAGCTGTAACTCACAATAGTTCAATTAAAGTAGAAAATGAAATTGATTCTGCTCAATTTCTGAAGAAGAGAAGTCGGAAAGCTGAATCCGATGAAATTGCATTTGCTCCAAAATCGCCAGATTCATCGAAATCTGTAAAGAAGAGGAAGCAGGAAGGTGAAAAAACGGCTGAATTGAAGAAGAGAAATGGGAAGGGAGATTCCCTTAAGGTTTCGTTCGCTCCGACGTCGCCGGATCAATCGGAAACTAAGAAGAGAAAGAGGAAGAATGAGGTCAAGGTTGAGAAAACGGTTGTTACAAGAGCTGGGAGAAAAGGTGGAAAGATGGCGAAGGTGCAGTATTACAAGAAAGTTGTTTATGACGGCGGTGAATTTGAAGTTGGGGATGATGTTTATGTGAAGAGGAGAGAAGATGCTACTTCTGATGAGGAAGATCCAGAAGTGGAGGAATGTAGGTTTTGTTTCCGTTCTGGGGACGAAATCATGATTGAGTGTGATAGTTGTTTAGGTGGGTTTCATTTGAAATGTTTGACACCACCGTTGAAAGATGTTCCTGAAGGGGATTGGATATGTGGGATCTGTGAGGGTCGTAAGATGGGTAAAGATGTTGATTTTCCAAAGCCGCCGAAGGGTAAGAAGCTTGTTAGAACAATGCGTCAGAGGCTTCAATCAAGTGATTTGTGGGCTGCTCGCATTGAGAGGTATGCTATATCTTTTATATATCAATTATATTATTTTATGTTTTTTAATAATAATTATTTGTACGCCAATTATGCGGTTTATTTACATCATTAGTTCAATGTTAGCAGTATATGGAAAGAAGTGGATGGCAGCTACTGGTGTCGGGTACGGTGGTATATGATCCCAGAAGAGACTTCTGTTGGGCGACAACCACATAATTTGAGCAGAGAGCTATATCGGACCAACGATTTGGCCAACATCGAGGTGATGGATTGATAATGATTTGGTTGTGGTATACTATATTAGAAAGGTGGTTAGTATGAATGGAAGTTGTCCTAGTTGTACTTCCAATCTATCAAAGAGCATATTGAATTTAGCATGTTTAGTGACAATCCTTGATAACTTTGGAACATCTAGATGGCTTGAGTTTTCTCCTCCACAGTGCATACTCAATTAAAGTCTGTCTGAATCTTACTCTGGAAAAGAGTACTCGTGGTATCTAGATGTACCAAGAAATAATAGAACTATAGTTTAATTGAGTAGATAATAGCTGTCGTCAATTATGGACTGATTGCTTACTTTGATAGATTGATTGCATTCTTGATTAGTTTAGCTTAGCATTTCTACGATCACGTCTCTACTTACAGGTCAACTTTTATATGCCTTAATGGCTCTTATCAAACTTAGCTTAGCATTTCTACGATCACGTCTCTACTTACAGGTCAACTTTTATATGCCTTAATGGCTCTTATCAAACTTTAGCATCCTTGGGCTGGTTGACTTGTTTATGCTGATAACTTCGATTATATTATGCAATGTCATACCCATATTAATTTAAATAGTTGGAGAAGTAGTTCAACTATTAAGTTCTACTGTGCTCCTGATTATTCGTTTCCTTCTTTTTGCATTCAATTTAATCAAATACAGTAAAAAAAGGGGACTAGAGTTATAATAATGAAATAGCTTGTGTATATAACACTTGTATTACTAAATGAAATAGAAAACTCCATCCTAAAATGTTGGAAATACATTGAAATTTATCCTTGAATATTGACAAGCTCACATTCGTCTTCTTTAGAAGAAGGAAATGGCATCTTTTGGAAACCTTAGAATTGGTTTTTATCTATTCTTGGAAGATGCCACTCATTATCTCCGGTAAAGTGGGTTTTATGAAGAGTTGAGAGAAACCAAGCCCAAAAAATGTGAGTGAATCTTTAGGTAAATGTTAAGTATCACTTCTGTAGTGATTATCATTCCATGTAACTTTTGAGTAGGATATCGCAGCTTGTAGGACGAGTCATGATGCACAACTAGTGGAGGGTTTTCCAAATTTGATAGAATTTGTGATCTATTATTGAGATAATAACAATTTTGTTGAAATGTTTATGTGAGTGTAGGCAAGAGGTGCCAAAAAATGTAAATTATGTTGCTTTTATGTGTGTTTTCATCTTCGATCCTTAGTGCGTGTAATTTTTCCCCATCATTAAAATTGAGCATAACTTGTGGTGCTGTATTATTTGTATTTCTATAGTCTCGTTTTTTAATGTTCAGGGTAATCAGAACTCATTCTCCTTTTTTCTCATCGTGGTGCTTCTCTGTTCAATATAGATGGAATCTGTCCTTCGACATTGCTATGTCATGATCCCTAAGGAGTATGCCAAAGCTAGCAATGAGGGAGATGATGTTTTCTTGTGTGAATATGAATATGACATCCAGTGGCACAGTTTCAAACGCTTGGCTGACATTGATGATGAAAGAGAGGTAACCGTCTTATAAAATATGTACAAGGACCATTCTTCTCATAAAAGAGTTAACCATTTAGCAGTGGTAATATTGACGACTTTATACAGATACACAGTTGAATGGTGATGAGACTGATTATTTAGCTGTGCTAGTACTGATGACTGTATATTTTTACATGGTAGAATTCTGATGAGAGTGACGGTGACGAAGACTGGAACATCGACAAGGAATCAGACTCTGACACAGATGAAGATGTTGAATATGATGAGGAAAATATTAAAATTTCACAATCGCAACCACCGACAAGCCATCAGTTAGCTGCAGTATGAACATTATTGTTCACTTGTATTAATTTGTATTATTTTTTGTGTTTATGAGTTCCTATGAAATTTTTGTTGTGTAAATGATACAGAATCTGCACAAGGGACGGTTCTTTGGACTTCAGAAGATAGGCACAAAAAGAATTCCAGAGCATATAAGGTCTCACAAACAGACTAATCTTGAAAGAGCAAAGGCATCATTGCTGTTAGCTTCGCTGCCTAAGTCTTTACCTTGTAGAAATAAGTATGTTGAAGTTTAACATGGTTTTTTAACATTTCATTTATCTATCCATGTTAAGCGACTATTACTAATCAAAACATGCTTTTATTTAGAGAAATGGACGAGATAACGACATTCATCAAAGGTGCTATTTCTGACAATCAATGTCTGGGGCGTTGCCTCTATATTCACGGTGTTCCGGGCACTGGCAAGGTACTATCTTCTAGAACTATCTACATTGCTTGTTGGTCTAATATCCAGCAGAACTTTAGTTTAGATATCACGGAGCAATTCTTATTATATTACTCCTTTTTTATGTGTGTAACGGCAGACAATGAGTGTACTCTCAGTGATGAGGAGTTTGAAGTCAGAAGTTGATGCAGGAAACATCAAACCATACTGTTTCGTGGAGATCAATGGTCTGAAATTGGCTTCACCGGAGAATATTTACAAGGTGAAGTTCACAAATCTTTTACTGTTTTTGGTTTTATGACCAAATTCCTTGGTTGCCTTATGGTTCCGACTTCCAATACTATTTTAGGTCATATATGAAGCATTAAATGGACACAGGGTTGGATGGAAAGAGGCTCTCCGTTTGCTAAATGAGAGGTTTGTAGAAGGGAAGAAAACTGGAGAAGAGGCTGACCGACCATGTATTTTGCTCATTGACGAACTTGATCTTCTTGTAACCAGAAACCAGTCGGTAACTAACAAATCCTGTTGCAAGAAACTTGTCATTAGGTGTCCACGTTATTTATTATATGAAACAAGATTGGCTATAACTTTCCTTTTATCACAGGTTCTGTACAATATTCTTGACTGGCCAACCAAGCCACATTCCAAGCTAATTGTGATAGGTAAGATTTAAGCTCTTGTGAATTATATTTATAGAATTGTGATCTTCTAGCTAAACAGATGTAATATCAAATACTGGGATCACAAGAATTCATATGATTATAATAAGAACTACAATGCCTTTGTGGACAACAAAAACTTAATTTTTGTAATAATGTACTGAATTCAGGGATAGCAAATACAATGGATCTTCCAGAGAAGTTACTTCCTCGTATATCAAGCCGAATGGGCATCCAGAGGCTTTGCTTTGCCCCATATAATTATCAGCAGCTTCAAGAAATCATTTCGAGTCGCCTCAATGGAATTGATATATTTGAAAAACAAGCTATGGAATTTGCTTCAAGAAAGTTTAGTCTCCAAGTCTTTTGAAGATTGGGTTCCTTTACCATTTTATGCACACGGGCACTTCTTTTGGTACAATAGCTTGTTGATTTACATCTGACATGATCTTTATTTCTTCAGGTTGCAGCTATATCAGGAGATGCACGCCGTGCTTTGGAAATATGCAGACGTGCAGCTGAAATTGCAGATTATCGAATGAAGAAGCAAGCTTCAGATCCTGATAATGTTGCTGCAGGTATAGTCAAAACCCATAGATTTTACGTGTCATCATAGGAGATAGAGGGATCACATGATGTTTCCTATTTCACATGTTGCCAATTAGTTACATGTGCCTTCACAAAAGTCTCATTTGTTTTTACACATTTGTAAACAATATGTGGATATAATAACCTAACAATAACCAAGTCAGATAAATCTTCACCCACATAAACAACTAGATTACTTGAGAACAAAACAATACACATTATTACAAAATTTATAGGATTTCAGGATCCAAGACCAAACTTCAGATTTTAATGTATTGTTTGCCCGATAATGTAAATCTAGTGTTCTCATTAATACTTATGCGGGTGCATCAGTATAATGAATTCTCCATTACATTGGTGTAATTTTATTAAAAGAAAAACATCGGTGTAATTTGATCCCTCTTCATAATCAATATAAGGTTAGTAGTCATATCACATAACTTGTATCTCAAACTGATTATGATGTAAAATATTTTAACAATTAATCAAATTATTTGTGTTAATAAGTTGATCAAATCTATTGGCAAGTGCCAACCACTAAGTTGTTTTCTTCAAGCACATAAATTATATAATCAGTAAACAAAAGTTCATGAATCATGGTATAAACTAAATAACCCAAATTATATAGTTATCATCTATCATAACCTCTACCTTTAGATGGTCTTGCTGATCCTTTAATGAATTACCATCAATTGACTCAAATTCCTGCCAGACTGAACTCTAGTCTGAAACTGATACCTACATTACCAAAATTGGAAATTGGGCCTCTTGAGTGTGCACGGCTACACCTGATTTCCCCAAGAGTTGCTTCACATTTAGCCATATATCTATAACTTCAGGAGGCACTTACATGTATCATATCAATGAATTGAAAACACACCTGCTGCTCCACAATAATTTTGTTGAAACTACCAGCATCAGTATCTCCAAAAAGAGTCTGTTACATTACCATGAGATTATGATAAACATGTCAACCATATTTTTTTGTCCACTTCAATATTTGTTATTCCGTTGTTACCTATGTGTGAAGGCTTGGTATTCAATGAGTTGGATCCAAATATGGTTTTTGATAGAACACTATGGCGTCATTTGATCCATGTAGCCGACCCCACATAGTGGGATAAGGCTTGGTTGTTGTTGTTGTTGTTGTATTTCATCCTTTTTCTTCTATAGTGGAATGTTTTCGCATGTTAACCTAAAATGCAGTCAGCATCACTTGTATGCACTGATACTTTATGTCTGAGGTAGTGTTTGAGCCCACTAATTCATTTGCATCTGCTCTCCAGGAAAGGGACTTGTTTGCATGGCAGACGTTGAATCAGCTATTCAAGAAATGTTCCAAGCACCTCATATTCAAGTGAGTTGAAATTTGCAGTGTGTGGATTTTTTTCCTTGACATTTCAAATATTTTTTTCCCCAAATGTTATAAGATTCCTAAGCAGCAGTCTTGACTGTGAACCAAGTCATGAGATGTAAAGTTATTAAATATACAATTAATACAAATGTTTCTGATGTAACTGAGATATCCACTTTTTTAAAAAGCAAAATGAGTGGTTTCTTTAACTGAGTATTATACCATGAACGAAGACTTTATGCTGTTTTATATGGCGCTGGCATTAGGTAATTAAGAAATAACTCACATGCTATTTTAGTTTATCAGATGTGACTGCTAGTCTTGTGGACCAGCGATCATAGGGCATAGGAAATTAATGTTTTCCTTTATCTTTAAGCCAGTTTTTTCTTGCGAATTTCATGAAATTACTTGAGGCAGGGTAATAAGCACTTTTATATATTCATTTAGCTGGCAGACTGACAGCATTTTATTTCAGTCTTACATCACATGCACCTTTTTTCGTTTTGAAGGTGATGAAAAACTGTTCCAGACTTGGCAAAATTTTCCTGACAGCTATGGTGCACGAGCTTTACAAAACAGGAATGGGGGAAACCACTTTTGAAAAGGTATGCTTCAGAAGTTTCTTTCTGTTGTTAACTTATTTTATTCTCGCTTGACATTCAGCATAAATTTTTCCACCTTAAAATTGAAGAGGTGAAATATGCTTACAACCTTTGTCCTCAGCTCTTTTTCAGTTTAATAGCATTATGGAAGTTTTTTATGTTGCATGTGACTCAAAATATATCAAATTACTTTTTTTTTTTGAAGCATAATATATCAAAAAAAATTTTTATTTATTTTAAGGATAATATATCAAATTTCTTGGTGAACTTAGATGTGTTATATCCTGGTGTTGACTTTCTAGTAGAAAAGGCATTGGTCAGAAGCTTAACATTAATTTGTTTAAGTTATCTTATGTAAAGTTTCATTTTAACAATGCAGTTGGCAATGACGGTTTCATGTCTCTGCGGCAGCAATGGTGAAGTGTTTCCTGGACATGATATCCTCCTGCAAGTTGGGTAAGACTTATGTTTGTTTCTTACATGTTTCTGCGTTGAAGTGGCCTGGAAGGGAGAACCGGGGGGTGGTGGTTGGGGGGGGGGGGGGGGGGGGGGGGGGTGTGTCTTGCTTTATACTCAAACACAAGTAACAGACTGTTTAACTGCTAAGTGTACGAGATCCATCCTAGTTGCATGTCTATTTTATCTAATAAGCCCTTCTTCCATTTTTTGCAGCTGTAAGTTGGGTGAGTGTAGGATCATTTTGTGTGAAGCAGGTGCCAAGCACAGGTTGCAAAAGTTGCAGCTCAATTTCCCGAGGTTATTTCTCAAAGCCCTTATTACTTCTTCAGCTGCCATTAATGATGCAAACCGATGA
Protein sequence

>MS.gene06093.t1

MASTPKKLLQSPSSKSNLRSQFSPLTPHSLPTRRSTRLNSDTPITPNLVAESQIQQSTPRRGRRIGENSVPAAKSRLDFTRKDKAVTHNSSIKVENEIDSAQFLKKRSRKAESDEIAFAPKSPDSSKSVKKRKQEGEKTAELKKRNGKGDSLKVSFAPTSPDQSETKKRKRKNEVKVEKTVVTRAGRKGGKMAKVQYYKKVVYDGGEFEVGDDVYVKRREDATSDEEDPEVEECRFCFRSGDEIMIECDSCLGGFHLKCLTPPLKDVPEGDWICGICEGRKMGKDVDFPKPPKGKKLVRTMRQRLQSSDLWAARIESIWKEVDGSYWCRVRWYMIPEETSVGRQPHNLSRELYRTNDLANIEMESVLRHCYVMIPKEYAKASNEGDDVFLCEYEYDIQWHSFKRLADIDDERENSDESDGDEDWNIDKESDSDTDEDVEYDEENIKISQSQPPTSHQLAANLHKGRFFGLQKIGTKRIPEHIRSHKQTNLERAKASLLLASLPKSLPCRNKEMDEITTFIKGAISDNQCLGRCLYIHGVPGTGKTMSVLSVMRSLKSEVDAGNIKPYCFVEINGLKLASPENIYKVIYEALNGHRVGWKEALRLLNERFVEGKKTGEEADRPCILLIDELDLLVTRNQSVLYNILDWPTKPHSKLIVIGIANTMDLPEKLLPRISSRMGIQRLCFAPYNYQQLQEIISSRLNGIDIFEKQAMEFASRKFSLQVAAISGDARRALEICRRAAEIADYRMKKQASDPDNVAAGKGLVCMADVESAIQEMFQAPHIQVMKNCSRLGKIFLTAMVHELYKTGMGETTFEKLAMTVSCLCGSNGEVFPGHDILLQVGCKLGECRIILCEAGAKHRLQKLQLNFPRLFLKALITSSAAINDANR