Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32995.t1 | XP_003589004.1 | 79.9 | 229 | 46 | 0 | 1 | 229 | 1 | 229 | 9.60E-58 | 233.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32995.t1 | G7I9Z4 | 79.9 | 229 | 46 | 0 | 1 | 229 | 1 | 229 | 6.9e-58 | 233.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059898 | MS.gene32995 | 0.818278 | 2.13E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32995.t1 | MTR_1g016270 | 92.391 | 276 | 21 | 0 | 1 | 276 | 1 | 276 | 2.70e-148 | 416 |
| MS.gene32995.t1 | MTR_8g097140 | 48.188 | 276 | 91 | 10 | 4 | 271 | 3 | 234 | 1.60e-51 | 169 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32995.t1 | AT5G60030 | 59.322 | 59 | 24 | 0 | 1 | 59 | 1 | 59 | 5.64e-17 | 79.0 |
| MS.gene32995.t1 | AT1G75335 | 59.322 | 59 | 24 | 0 | 1 | 59 | 1 | 59 | 9.39e-16 | 71.6 |
Find 64 sgRNAs with CRISPR-Local
Find 70 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAAAGAAGAAACTAGAATT+TGG | 0.282317 | 1.1:-5867611 | MS.gene32995:CDS |
| AAGAAGGAGAAGAGTGTAGA+AGG | 0.312453 | 1.1:-5867497 | MS.gene32995:CDS |
| AAGAAGAATGGAAGTGAGTT+AGG | 0.374907 | 1.1:-5867578 | MS.gene32995:CDS |
| TGAAAAGGAGAAGAATTTAG+AGG | 0.379641 | 1.1:-5867390 | MS.gene32995:CDS |
| GTTGTCAGCAGAGACAAATT+TGG | 0.400587 | 1.1:+5867868 | None:intergenic |
| ATTCTCGGGATGTAGAAATT+AGG | 0.415570 | 1.1:-5867280 | MS.gene32995:CDS |
| AGAAGGAGAAGAGTGTAGAA+GGG | 0.423276 | 1.1:-5867496 | MS.gene32995:CDS |
| GAAGACACGGAAGAGAGATC+TGG | 0.440783 | 1.1:-5867173 | MS.gene32995:CDS |
| GTGGAGGGCTAGTTGATTCT+CGG | 0.450550 | 1.1:-5867295 | MS.gene32995:CDS |
| AAGGAGAAGAATTTAGAGGT+TGG | 0.455722 | 1.1:-5867386 | MS.gene32995:CDS |
| TTAAACCGGCGCAGTCTCAT+AGG | 0.455996 | 1.1:-5867766 | MS.gene32995:CDS |
| TGAGCTTAATCAACTTCATA+AGG | 0.457180 | 1.1:-5867792 | MS.gene32995:CDS |
| AGTCTAAGAAGAAAATGAAA+AGG | 0.460042 | 1.1:-5867145 | MS.gene32995:CDS |
| ATGTGAAGGTTGAAAGTAAA+GGG | 0.462456 | 1.1:-5867469 | MS.gene32995:CDS |
| GGAGAGAGTGTAAAGAAGAA+TGG | 0.469789 | 1.1:-5867590 | MS.gene32995:CDS |
| CTCCTCAGGAGTTAGAAATT+AGG | 0.475036 | 1.1:-5867439 | MS.gene32995:CDS |
| GAGAAGCGTGATAAGAAAGA+TGG | 0.475565 | 1.1:-5867632 | MS.gene32995:CDS |
| AAATGAAGTGGGGAGTGAAA+AGG | 0.480460 | 1.1:-5867405 | MS.gene32995:CDS |
| AACAGATGATAGTGGTAGAT+TGG | 0.498404 | 1.1:-5867723 | MS.gene32995:CDS |
| TGGAGGGCTAGTTGATTCTC+GGG | 0.502825 | 1.1:-5867294 | MS.gene32995:CDS |
| AAGACACGGAAGAGAGATCT+GGG | 0.505868 | 1.1:-5867172 | MS.gene32995:CDS |
| ACTTCATAAGGAGCTTAAAC+CGG | 0.507615 | 1.1:-5867780 | MS.gene32995:CDS |
| GAATGGAAGTGAGTTAGGTC+AGG | 0.510402 | 1.1:-5867573 | MS.gene32995:CDS |
| GAAGTTGTTGGTTCTGTCGT+GGG | 0.512214 | 1.1:-5867662 | MS.gene32995:CDS |
| GAAGGAGAAGAGTGTAGAAG+GGG | 0.517307 | 1.1:-5867495 | MS.gene32995:CDS |
| CGAGAAGAAGAACAAGAAGA+AGG | 0.521154 | 1.1:-5867513 | MS.gene32995:CDS |
| TAAAGGGATAGTTCCTCCTC+AGG | 0.521560 | 1.1:-5867453 | MS.gene32995:CDS |
| TTTAGAGGTTGGAAATGTGA+AGG | 0.536582 | 1.1:-5867375 | MS.gene32995:CDS |
| TGAAGTTGTTGGTTCTGTCG+TGG | 0.542751 | 1.1:-5867663 | MS.gene32995:CDS |
| AATGTGAAGGTTGAAAGTAA+AGG | 0.544852 | 1.1:-5867470 | MS.gene32995:CDS |
| AGACGGAGCAAAGGAAGAAG+AGG | 0.546821 | 1.1:-5867202 | MS.gene32995:CDS |
| CATTGAAAGCCGTGGATGCA+CGG | 0.548708 | 1.1:+5867813 | None:intergenic |
| AAGAGGGAAAGACGGAGCAA+AGG | 0.550638 | 1.1:-5867211 | MS.gene32995:CDS |
| AAACCTGGTCGTGAAGTTGT+TGG | 0.551304 | 1.1:-5867674 | MS.gene32995:CDS |
| GAGTTAGGTCAGGACGATGA+AGG | 0.567638 | 1.1:-5867563 | MS.gene32995:CDS |
| GAGTAAATTACATGCTGAAG+AGG | 0.574245 | 1.1:-5867228 | MS.gene32995:CDS |
| AATGGTGTGAAGAGTGAAAG+TGG | 0.576054 | 1.1:-5867314 | MS.gene32995:CDS |
| GGGACGAGAACAACAGAAAG+AGG | 0.576625 | 1.1:-5867354 | MS.gene32995:CDS |
| TGTAGAAGGGGAGAATGTGA+AGG | 0.579317 | 1.1:-5867483 | MS.gene32995:CDS |
| TTAGAGGTTGGAAATGTGAA+GGG | 0.581388 | 1.1:-5867374 | MS.gene32995:CDS |
| GATTGGTCGAAAGTACTGTT+CGG | 0.581744 | 1.1:-5867706 | MS.gene32995:CDS |
| TACCACTATCATCTGTTGCA+TGG | 0.584555 | 1.1:+5867729 | None:intergenic |
| AGCCATGCAACAGATGATAG+TGG | 0.587057 | 1.1:-5867731 | MS.gene32995:CDS |
| ATTAAGCTCATTGAAAGCCG+TGG | 0.591302 | 1.1:+5867805 | None:intergenic |
| GACGATGAAGGAAGAACGGA+GGG | 0.595325 | 1.1:-5867551 | MS.gene32995:CDS |
| TTATCTGCTCCGTGCATCCA+CGG | 0.595538 | 1.1:-5867822 | MS.gene32995:CDS |
| AGTAAATTACATGCTGAAGA+GGG | 0.598011 | 1.1:-5867227 | MS.gene32995:CDS |
| GACCTAATTTCTAACTCCTG+AGG | 0.598620 | 1.1:+5867437 | None:intergenic |
| GAACCAACAACTTCACGACC+AGG | 0.602335 | 1.1:+5867671 | None:intergenic |
| GTTCGGAGTGATGTTAAACC+TGG | 0.607851 | 1.1:-5867689 | MS.gene32995:CDS |
| GTTGTTGGTTCTGTCGTGGG+TGG | 0.608189 | 1.1:-5867659 | MS.gene32995:CDS |
| ACGAGAACAACAGAAAGAGG+TGG | 0.612657 | 1.1:-5867351 | MS.gene32995:CDS |
| TCAGGACGATGAAGGAAGAA+CGG | 0.621358 | 1.1:-5867555 | MS.gene32995:CDS |
| ACATGCTGAAGAGGGAAAGA+CGG | 0.635975 | 1.1:-5867219 | MS.gene32995:CDS |
| TTCTTCCTATGAGACTGCGC+CGG | 0.636496 | 1.1:+5867761 | None:intergenic |
| GAAGAGGAAGAATGAAGACA+CGG | 0.643138 | 1.1:-5867186 | MS.gene32995:CDS |
| AAATTTGTCTCTGCTGACAA+CGG | 0.644626 | 1.1:-5867866 | MS.gene32995:CDS |
| CTAATTTCTAACTCCTGAGG+AGG | 0.647828 | 1.1:+5867440 | None:intergenic |
| GTGTGAAGAGTGAAAGTGGA+GGG | 0.650346 | 1.1:-5867310 | MS.gene32995:CDS |
| GGACGATGAAGGAAGAACGG+AGG | 0.651421 | 1.1:-5867552 | MS.gene32995:CDS |
| GAAAATGAAAAGGAAACACG+AGG | 0.691720 | 1.1:-5867135 | MS.gene32995:CDS |
| AAAATGAAAAGGAAACACGA+GGG | 0.733622 | 1.1:-5867134 | MS.gene32995:CDS |
| AGACACGGAAGAGAGATCTG+GGG | 0.742079 | 1.1:-5867171 | MS.gene32995:CDS |
| GGTGTGAAGAGTGAAAGTGG+AGG | 0.804724 | 1.1:-5867311 | MS.gene32995:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGTCTAAGAAGAAAATGAAA+AGG | - | chr1.1:5867923-5867942 | MS.gene32995:CDS | 25.0% |
| AAAATGAAAAGGAAACACGA+GGG | - | chr1.1:5867934-5867953 | MS.gene32995:CDS | 30.0% | |
| AATGTGAAGGTTGAAAGTAA+AGG | - | chr1.1:5867598-5867617 | MS.gene32995:CDS | 30.0% | |
| AGTAAATTACATGCTGAAGA+GGG | - | chr1.1:5867841-5867860 | MS.gene32995:CDS | 30.0% | |
| ATGTGAAGGTTGAAAGTAAA+GGG | - | chr1.1:5867599-5867618 | MS.gene32995:CDS | 30.0% | |
| GGAAAGAAGAAACTAGAATT+TGG | - | chr1.1:5867457-5867476 | MS.gene32995:CDS | 30.0% | |
| TCGAAAAAGAGAAATGAAGT+GGG | - | chr1.1:5867652-5867671 | MS.gene32995:CDS | 30.0% | |
| TGAAAAGGAGAAGAATTTAG+AGG | - | chr1.1:5867678-5867697 | MS.gene32995:CDS | 30.0% | |
| TGAGCTTAATCAACTTCATA+AGG | - | chr1.1:5867276-5867295 | MS.gene32995:CDS | 30.0% | |
| AAATTTGTCTCTGCTGACAA+CGG | - | chr1.1:5867202-5867221 | MS.gene32995:CDS | 35.0% | |
| AAGAAGAATGGAAGTGAGTT+AGG | - | chr1.1:5867490-5867509 | MS.gene32995:CDS | 35.0% | |
| AAGGAGAAGAATTTAGAGGT+TGG | - | chr1.1:5867682-5867701 | MS.gene32995:CDS | 35.0% | |
| ACTTCATAAGGAGCTTAAAC+CGG | - | chr1.1:5867288-5867307 | MS.gene32995:CDS | 35.0% | |
| ATTCTCGGGATGTAGAAATT+AGG | - | chr1.1:5867788-5867807 | MS.gene32995:CDS | 35.0% | |
| CGAAAAAGAGAAATGAAGTG+GGG | - | chr1.1:5867653-5867672 | MS.gene32995:CDS | 35.0% | |
| GAAAATGAAAAGGAAACACG+AGG | - | chr1.1:5867933-5867952 | MS.gene32995:CDS | 35.0% | |
| GAGTAAATTACATGCTGAAG+AGG | - | chr1.1:5867840-5867859 | MS.gene32995:CDS | 35.0% | |
| GTCGAAAAAGAGAAATGAAG+TGG | - | chr1.1:5867651-5867670 | MS.gene32995:CDS | 35.0% | |
| GTGGAGAAAAAACTAAGCAA+TGG | - | chr1.1:5867736-5867755 | MS.gene32995:CDS | 35.0% | |
| TTAGAGGTTGGAAATGTGAA+GGG | - | chr1.1:5867694-5867713 | MS.gene32995:CDS | 35.0% | |
| TTTAGAGGTTGGAAATGTGA+AGG | - | chr1.1:5867693-5867712 | MS.gene32995:CDS | 35.0% | |
| ! | CGAAAAAGAAGCATGAAACT+GGG | - | chr1.1:5867812-5867831 | MS.gene32995:CDS | 35.0% |
| ! | TCGAAAAAGAAGCATGAAAC+TGG | - | chr1.1:5867811-5867830 | MS.gene32995:CDS | 35.0% |
| !! | AACAGATGATAGTGGTAGAT+TGG | - | chr1.1:5867345-5867364 | MS.gene32995:CDS | 35.0% |
| AAATGAAGTGGGGAGTGAAA+AGG | - | chr1.1:5867663-5867682 | MS.gene32995:CDS | 40.0% | |
| AATGGTGTGAAGAGTGAAAG+TGG | - | chr1.1:5867754-5867773 | MS.gene32995:CDS | 40.0% | |
| ATTAAGCTCATTGAAAGCCG+TGG | + | chr1.1:5867266-5867285 | None:intergenic | 40.0% | |
| CGAGAAGAAGAACAAGAAGA+AGG | - | chr1.1:5867555-5867574 | MS.gene32995:CDS | 40.0% | |
| CTAATTTCTAACTCCTGAGG+AGG | + | chr1.1:5867631-5867650 | None:intergenic | 40.0% | |
| CTCCTCAGGAGTTAGAAATT+AGG | - | chr1.1:5867629-5867648 | MS.gene32995:CDS | 40.0% | |
| GAAGAGGAAGAATGAAGACA+CGG | - | chr1.1:5867882-5867901 | MS.gene32995:CDS | 40.0% | |
| GACCTAATTTCTAACTCCTG+AGG | + | chr1.1:5867634-5867653 | None:intergenic | 40.0% | |
| GTTGTCAGCAGAGACAAATT+TGG | + | chr1.1:5867203-5867222 | None:intergenic | 40.0% | |
| TACCACTATCATCTGTTGCA+TGG | + | chr1.1:5867342-5867361 | None:intergenic | 40.0% | |
| ! | AAGAAGGAGAAGAGTGTAGA+AGG | - | chr1.1:5867571-5867590 | MS.gene32995:CDS | 40.0% |
| ! | AGAAGGAGAAGAGTGTAGAA+GGG | - | chr1.1:5867572-5867591 | MS.gene32995:CDS | 40.0% |
| ! | GAGAAGCGTGATAAGAAAGA+TGG | - | chr1.1:5867436-5867455 | MS.gene32995:CDS | 40.0% |
| ! | GGAGAGAGTGTAAAGAAGAA+TGG | - | chr1.1:5867478-5867497 | MS.gene32995:CDS | 40.0% |
| !! | GATTGGTCGAAAGTACTGTT+CGG | - | chr1.1:5867362-5867381 | MS.gene32995:CDS | 40.0% |
| AAACCTGGTCGTGAAGTTGT+TGG | - | chr1.1:5867394-5867413 | MS.gene32995:CDS | 45.0% | |
| AAGACACGGAAGAGAGATCT+GGG | - | chr1.1:5867896-5867915 | MS.gene32995:CDS | 45.0% | |
| ACATGCTGAAGAGGGAAAGA+CGG | - | chr1.1:5867849-5867868 | MS.gene32995:CDS | 45.0% | |
| ACGAGAACAACAGAAAGAGG+TGG | - | chr1.1:5867717-5867736 | MS.gene32995:CDS | 45.0% | |
| AGCCATGCAACAGATGATAG+TGG | - | chr1.1:5867337-5867356 | MS.gene32995:CDS | 45.0% | |
| GAATGGAAGTGAGTTAGGTC+AGG | - | chr1.1:5867495-5867514 | MS.gene32995:CDS | 45.0% | |
| GTGTGAAGAGTGAAAGTGGA+GGG | - | chr1.1:5867758-5867777 | MS.gene32995:CDS | 45.0% | |
| GTTCGGAGTGATGTTAAACC+TGG | - | chr1.1:5867379-5867398 | MS.gene32995:CDS | 45.0% | |
| TAAAGGGATAGTTCCTCCTC+AGG | - | chr1.1:5867615-5867634 | MS.gene32995:CDS | 45.0% | |
| TCAGGACGATGAAGGAAGAA+CGG | - | chr1.1:5867513-5867532 | MS.gene32995:CDS | 45.0% | |
| TGTAGAAGGGGAGAATGTGA+AGG | - | chr1.1:5867585-5867604 | MS.gene32995:CDS | 45.0% | |
| ! | GAAGGAGAAGAGTGTAGAAG+GGG | - | chr1.1:5867573-5867592 | MS.gene32995:CDS | 45.0% |
| !! | GAAGTTGTTGGTTCTGTCGT+GGG | - | chr1.1:5867406-5867425 | MS.gene32995:CDS | 45.0% |
| !! | TGAAGTTGTTGGTTCTGTCG+TGG | - | chr1.1:5867405-5867424 | MS.gene32995:CDS | 45.0% |
| AAGAGGGAAAGACGGAGCAA+AGG | - | chr1.1:5867857-5867876 | MS.gene32995:CDS | 50.0% | |
| AGACACGGAAGAGAGATCTG+GGG | - | chr1.1:5867897-5867916 | MS.gene32995:CDS | 50.0% | |
| AGACGGAGCAAAGGAAGAAG+AGG | - | chr1.1:5867866-5867885 | MS.gene32995:CDS | 50.0% | |
| CATTGAAAGCCGTGGATGCA+CGG | + | chr1.1:5867258-5867277 | None:intergenic | 50.0% | |
| GAACCAACAACTTCACGACC+AGG | + | chr1.1:5867400-5867419 | None:intergenic | 50.0% | |
| GAAGACACGGAAGAGAGATC+TGG | - | chr1.1:5867895-5867914 | MS.gene32995:CDS | 50.0% | |
| GACGATGAAGGAAGAACGGA+GGG | - | chr1.1:5867517-5867536 | MS.gene32995:CDS | 50.0% | |
| GAGTTAGGTCAGGACGATGA+AGG | - | chr1.1:5867505-5867524 | MS.gene32995:CDS | 50.0% | |
| GGGACGAGAACAACAGAAAG+AGG | - | chr1.1:5867714-5867733 | MS.gene32995:CDS | 50.0% | |
| GGTGTGAAGAGTGAAAGTGG+AGG | - | chr1.1:5867757-5867776 | MS.gene32995:CDS | 50.0% | |
| TTAAACCGGCGCAGTCTCAT+AGG | - | chr1.1:5867302-5867321 | MS.gene32995:CDS | 50.0% | |
| TTATCTGCTCCGTGCATCCA+CGG | - | chr1.1:5867246-5867265 | MS.gene32995:CDS | 50.0% | |
| TTCTTCCTATGAGACTGCGC+CGG | + | chr1.1:5867310-5867329 | None:intergenic | 50.0% | |
| !! | GTGGAGGGCTAGTTGATTCT+CGG | - | chr1.1:5867773-5867792 | MS.gene32995:CDS | 50.0% |
| !! | TGGAGGGCTAGTTGATTCTC+GGG | - | chr1.1:5867774-5867793 | MS.gene32995:CDS | 50.0% |
| GGACGATGAAGGAAGAACGG+AGG | - | chr1.1:5867516-5867535 | MS.gene32995:CDS | 55.0% | |
| !! | GTTGTTGGTTCTGTCGTGGG+TGG | - | chr1.1:5867409-5867428 | MS.gene32995:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 5867130 | 5867960 | 5867130 | ID=MS.gene32995 |
| chr1.1 | mRNA | 5867130 | 5867960 | 5867130 | ID=MS.gene32995.t1;Parent=MS.gene32995 |
| chr1.1 | exon | 5867130 | 5867960 | 5867130 | ID=MS.gene32995.t1.exon1;Parent=MS.gene32995.t1 |
| chr1.1 | CDS | 5867130 | 5867960 | 5867130 | ID=cds.MS.gene32995.t1;Parent=MS.gene32995.t1 |
| Gene Sequence |
| Protein sequence |
