AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene059898


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059898.t1 MTR_2g080990 97.087 103 3 0 1 103 1 103 6.75e-66 194
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 13 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCGGCAGACTTCTCATTTG+CGG 0.410034 2.2:-15076652 None:intergenic
GGAGCTAAAATCAAGGTTGC+TGG 0.433275 2.2:+15074670 MS.gene059898:CDS
GCAATGGATACATCAGAATC+AGG 0.468029 2.2:+15074649 MS.gene059898:intron
GAGTATACCTTGAGGTGCTT+CGG 0.483494 2.2:-15074487 None:intergenic
GTCGGTTATGTCCATCGAAA+CGG 0.525995 2.2:-15074454 None:intergenic
AACAGGAAGGTGAAGCAGAA+TGG 0.552894 2.2:+15076570 MS.gene059898:intron
TTCAGACTTGGAAACTTTCG+CGG 0.568580 2.2:-15076670 None:intergenic
AGAATCAGGAGCTAAAATCA+AGG 0.594733 2.2:+15074663 MS.gene059898:CDS
GGAAGGTGAAGCAGAATGGA+AGG 0.604858 2.2:+15076574 MS.gene059898:intron
ATCGTTTCCGAAGCACCTCA+AGG 0.606213 2.2:+15074480 MS.gene059898:CDS
GCTAAAATCAAGGTTGCTGG+TGG 0.607057 2.2:+15074673 MS.gene059898:CDS
TGCTTCGGAAACGATGGTGT+CGG 0.636322 2.2:-15074472 None:intergenic
TTGAGGTGCTTCGGAAACGA+TGG 0.645225 2.2:-15074478 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTATTATAATTTTTTAAAGA+AGG + chr2.2:15075552-15075571 MS.gene059898:intron 10.0%
!! AAAAACAAGAAGAATAAAAA+CGG + chr2.2:15074429-15074448 MS.gene059898:CDS 15.0%
!! AATAAAGAAAACTTACAAAA+TGG - chr2.2:15074704-15074723 None:intergenic 15.0%
!! TTAAACCACAAAAAAAAAAA+AGG - chr2.2:15074603-15074622 None:intergenic 15.0%
!!! ATTTTCCTTTTTTTTTTTTG+TGG + chr2.2:15074595-15074614 MS.gene059898:intron 15.0%
!!! TTATAATTTTTTAAAGAAGG+CGG + chr2.2:15075555-15075574 MS.gene059898:intron 15.0%
!! AAATCAAACTTATTGGATTA+AGG - chr2.2:15075037-15075056 None:intergenic 20.0%
!! AATCAAACTTATTGGATTAA+GGG - chr2.2:15075036-15075055 None:intergenic 20.0%
!! TTAATCCAATAAGTTTGATT+TGG + chr2.2:15075036-15075055 MS.gene059898:intron 20.0%
!! TTGCATAATAATAGTTGTAT+GGG + chr2.2:15076231-15076250 MS.gene059898:intron 20.0%
!! TTTGCATAATAATAGTTGTA+TGG + chr2.2:15076230-15076249 MS.gene059898:intron 20.0%
!!! CATAAAGTTTTAAACAATGA+TGG - chr2.2:15076384-15076403 None:intergenic 20.0%
!!! CATCATTGTTTAAAACTTTA+TGG + chr2.2:15076382-15076401 MS.gene059898:intron 20.0%
!!! TTAATTTGTTTATCCAAACA+AGG - chr2.2:15074898-15074917 None:intergenic 20.0%
! AAATACCAACTTAGAATATG+AGG - chr2.2:15075145-15075164 None:intergenic 25.0%
! AATGATTGTTTGATAGAACA+AGG + chr2.2:15076045-15076064 MS.gene059898:intron 25.0%
! ACAGTTAACATATAACTCAT+AGG - chr2.2:15075958-15075977 None:intergenic 25.0%
! AGTCTATCAATAGGAAAATT+TGG + chr2.2:15076195-15076214 MS.gene059898:intron 25.0%
! TAGAGAATCACAAGTTAAAT+GGG + chr2.2:15074770-15074789 MS.gene059898:intron 25.0%
! TTCTATCAAACAATCATTCA+AGG - chr2.2:15076043-15076062 None:intergenic 25.0%
!! AACCAAAGCATTAAAAAACT+AGG - chr2.2:15074827-15074846 None:intergenic 25.0%
!! ACCAAAGCATTAAAAAACTA+GGG - chr2.2:15074826-15074845 None:intergenic 25.0%
!! TATTATGTTTTGCAAGTAGA+TGG - chr2.2:15076457-15076476 None:intergenic 25.0%
!! TTTAAAACATATATGTGCGT+AGG - chr2.2:15075590-15075609 None:intergenic 25.0%
!!! AAAAAAGTGTCATCAAAATG+AGG - chr2.2:15075879-15075898 None:intergenic 25.0%
!!! AAAAAGTGTCATCAAAATGA+GGG - chr2.2:15075878-15075897 None:intergenic 25.0%
!!! ACTTTTTTTGTTTTCAGACT+TGG - chr2.2:15076685-15076704 None:intergenic 25.0%
!!! TTTTTTAATGCTTTGGTTGA+TGG + chr2.2:15074829-15074848 MS.gene059898:intron 25.0%
AAAACTGATGAACAATTCAC+AGG - chr2.2:15076284-15076303 None:intergenic 30.0%
AATATTCTATACTTTCGCAG+AGG + chr2.2:15076309-15076328 MS.gene059898:intron 30.0%
ACTTAGAATATGAGGAGATA+TGG - chr2.2:15075137-15075156 None:intergenic 30.0%
AGAGAATCACAAGTTAAATG+GGG + chr2.2:15074771-15074790 MS.gene059898:intron 30.0%
AGCCAAATAGAGAGTTAAAT+AGG + chr2.2:15076102-15076121 MS.gene059898:intron 30.0%
ATAACTGACAATAGTTAGCT+AGG + chr2.2:15076155-15076174 MS.gene059898:intron 30.0%
ATATCAATCGAGTCTATGAT+AGG + chr2.2:15076075-15076094 MS.gene059898:intron 30.0%
ATCACTTAACTGTGTGATAT+AGG + chr2.2:15075222-15075241 MS.gene059898:intron 30.0%
ATCATAGACTCGATTGATAT+CGG - chr2.2:15076075-15076094 None:intergenic 30.0%
ATTCAAGGCAAAAATTCTGA+TGG - chr2.2:15076028-15076047 None:intergenic 30.0%
CATGTTATAATTGAGGACAT+TGG - chr2.2:15075990-15076009 None:intergenic 30.0%
CCAAATAGAGAGTTAAATAG+GGG + chr2.2:15076104-15076123 MS.gene059898:intron 30.0%
CTTACAAAATTGATCAGCAT+TGG - chr2.2:15075769-15075788 None:intergenic 30.0%
CTTATTCATAACCACTCATA+TGG - chr2.2:15074934-15074953 None:intergenic 30.0%
GAATACGAACAATTCACTAA+TGG + chr2.2:15075734-15075753 MS.gene059898:intron 30.0%
GACGAAGGCTAAAAATATAA+GGG - chr2.2:15074728-15074747 None:intergenic 30.0%
GCCAAATAGAGAGTTAAATA+GGG + chr2.2:15076103-15076122 MS.gene059898:intron 30.0%
GTAGAGAATCACAAGTTAAA+TGG + chr2.2:15074769-15074788 MS.gene059898:intron 30.0%
TATCTCCTCATATTCTAAGT+TGG + chr2.2:15075137-15075156 MS.gene059898:intron 30.0%
TGTTCGTATTCTAATTACAC+AGG - chr2.2:15075725-15075744 None:intergenic 30.0%
TTGCACCAAATCAAACTTAT+TGG - chr2.2:15075044-15075063 None:intergenic 30.0%
! ATAGTAGTTGCCTTGTATTT+TGG + chr2.2:15075493-15075512 MS.gene059898:intron 30.0%
! ATTGGATGTTGTTGTTGAAT+TGG + chr2.2:15075094-15075113 MS.gene059898:intron 30.0%
! TAGTAGTTGCCTTGTATTTT+GGG + chr2.2:15075494-15075513 MS.gene059898:intron 30.0%
! TTGGATGTTGTTGTTGAATT+GGG + chr2.2:15075095-15075114 MS.gene059898:intron 30.0%
!! AACACTTTTTGATCTCTTCA+TGG - chr2.2:15076601-15076620 None:intergenic 30.0%
!! AATCACATTTACTTTTGCAC+TGG + chr2.2:15075363-15075382 MS.gene059898:intron 30.0%
!! ACACTTTTTGATCTCTTCAT+GGG - chr2.2:15076600-15076619 None:intergenic 30.0%
!! CTTTTGTTTATTTGCAGCAA+TGG + chr2.2:15074633-15074652 MS.gene059898:intron 30.0%
!! TATTCTTTAAAGCATGCACT+TGG + chr2.2:15075443-15075462 MS.gene059898:intron 30.0%
!! TGTATGGGATATTTGTTATC+AGG + chr2.2:15076246-15076265 MS.gene059898:intron 30.0%
!!! ACCCTAGTTTTTTAATGCTT+TGG + chr2.2:15074822-15074841 MS.gene059898:intron 30.0%
!!! GCAATGTAGTTTTTTTTGCA+TGG + chr2.2:15075321-15075340 MS.gene059898:intron 30.0%
AATCTCACATGTGAATATGC+TGG + chr2.2:15074968-15074987 MS.gene059898:intron 35.0%
ACTGTGTGATATAGGAAATC+TGG + chr2.2:15075230-15075249 MS.gene059898:intron 35.0%
AGAATCAGGAGCTAAAATCA+AGG + chr2.2:15074663-15074682 MS.gene059898:CDS 35.0%
AGTCATCATTCTTTGTCGAA+CGG + chr2.2:15075194-15075213 MS.gene059898:intron 35.0%
AGTTGAACGCAATTATAGGA+GGG - chr2.2:15075264-15075283 None:intergenic 35.0%
ATCTGTTGAGTTCTTGAAGA+AGG + chr2.2:15075419-15075438 MS.gene059898:intron 35.0%
CAAAGCAAACCCAAAATACA+AGG - chr2.2:15075506-15075525 None:intergenic 35.0%
CCAATAATCAGTTGAGTAAC+TGG - chr2.2:15075079-15075098 None:intergenic 35.0%
CCAGTTACTCAACTGATTAT+TGG + chr2.2:15075076-15075095 MS.gene059898:intron 35.0%
CCATTGCTACAATGAAGAAA+TGG - chr2.2:15075302-15075321 None:intergenic 35.0%
CCATTTCTTCATTGTAGCAA+TGG + chr2.2:15075299-15075318 MS.gene059898:intron 35.0%
CCCCTATTTAACTCTCTATT+TGG - chr2.2:15076107-15076126 None:intergenic 35.0%
CGACGAAGGCTAAAAATATA+AGG - chr2.2:15074729-15074748 None:intergenic 35.0%
CTGTGTGATATAGGAAATCT+GGG + chr2.2:15075231-15075250 MS.gene059898:intron 35.0%
GCAACGATTTGAGAAAATGT+TGG - chr2.2:15074867-15074886 None:intergenic 35.0%
GTACAGTTGAACGCAATTAT+AGG - chr2.2:15075268-15075287 None:intergenic 35.0%
TAATCAGTTGAGTAACTGGA+CGG - chr2.2:15075075-15075094 None:intergenic 35.0%
TCATAGACTCGATTGATATC+GGG - chr2.2:15076074-15076093 None:intergenic 35.0%
TCGCGAAGTATATTACAGAA+GGG - chr2.2:15076341-15076360 None:intergenic 35.0%
TCTGTTGAGTTCTTGAAGAA+GGG + chr2.2:15075420-15075439 MS.gene059898:intron 35.0%
TTACACAGGCAATTTAGTGA+TGG - chr2.2:15075711-15075730 None:intergenic 35.0%
TTCTCAAATCGTTGCAATTC+AGG + chr2.2:15074872-15074891 MS.gene059898:intron 35.0%
TTTGCAAGTAGATGGAAATC+AGG - chr2.2:15076449-15076468 None:intergenic 35.0%
! ATAATGTTTTCTGCTCTGCT+GGG - chr2.2:15075684-15075703 None:intergenic 35.0%
! TTGCTCTTCTGTTTTGTCAT+GGG + chr2.2:15075928-15075947 MS.gene059898:intron 35.0%
! TTTGCTCTTCTGTTTTGTCA+TGG + chr2.2:15075927-15075946 MS.gene059898:intron 35.0%
!! TTACTTTTGCACTGGATGTT+AGG + chr2.2:15075371-15075390 MS.gene059898:intron 35.0%
AAGTTAAATGGGGTTTAGCG+CGG + chr2.2:15074781-15074800 MS.gene059898:intron 40.0%
ACTGATGAACAATTCACAGG+AGG - chr2.2:15076281-15076300 None:intergenic 40.0%
AGTTAGCTAGGAAGAGTAGA+TGG + chr2.2:15076167-15076186 MS.gene059898:intron 40.0%
AGTTCAGTTCGCATAGTTGA+TGG + chr2.2:15075824-15075843 MS.gene059898:intron 40.0%
ATGGATGGGAGTCTATCAAT+AGG + chr2.2:15076186-15076205 MS.gene059898:intron 40.0%
CAGTTGAACGCAATTATAGG+AGG - chr2.2:15075265-15075284 None:intergenic 40.0%
CTCGCGAAGTATATTACAGA+AGG - chr2.2:15076342-15076361 None:intergenic 40.0%
GCAATGGATACATCAGAATC+AGG + chr2.2:15074649-15074668 MS.gene059898:intron 40.0%
GTGTTTACTCCACGACATAT+TGG - chr2.2:15076413-15076432 None:intergenic 40.0%
TCACATGTGAATATGCTGGT+AGG + chr2.2:15074972-15074991 MS.gene059898:intron 40.0%
TCCAGCGCATGTTATAATTG+AGG - chr2.2:15075997-15076016 None:intergenic 40.0%
TCCTCAATTATAACATGCGC+TGG + chr2.2:15075993-15076012 MS.gene059898:intron 40.0%
TGAAAAGCGAGTATACCTTG+AGG - chr2.2:15074498-15074517 None:intergenic 40.0%
TTCAGACTTGGAAACTTTCG+CGG - chr2.2:15076673-15076692 None:intergenic 40.0%
! GATAATGTTTTCTGCTCTGC+TGG - chr2.2:15075685-15075704 None:intergenic 40.0%
!! TGGATGTTAGGTTCTAGACT+TGG + chr2.2:15075383-15075402 MS.gene059898:intron 40.0%
AACAATTCACAGGAGGTTCG+AGG - chr2.2:15076274-15076293 None:intergenic 45.0%
AACAGGAAGGTGAAGCAGAA+TGG + chr2.2:15076570-15076589 MS.gene059898:intron 45.0%
ACGCAATTATAGGAGGGAAG+AGG - chr2.2:15075258-15075277 None:intergenic 45.0%
AGCTAGGAAGAGTAGATGGA+TGG + chr2.2:15076171-15076190 MS.gene059898:intron 45.0%
CGCAATTATAGGAGGGAAGA+GGG - chr2.2:15075257-15075276 None:intergenic 45.0%
GCTAAAATCAAGGTTGCTGG+TGG + chr2.2:15074673-15074692 MS.gene059898:CDS 45.0%
GCTAGGAAGAGTAGATGGAT+GGG + chr2.2:15076172-15076191 MS.gene059898:intron 45.0%
GGAGCTAAAATCAAGGTTGC+TGG + chr2.2:15074670-15074689 MS.gene059898:CDS 45.0%
GTAGATGGAAATCAGGAAGC+AGG - chr2.2:15076442-15076461 None:intergenic 45.0%
GTCGGTTATGTCCATCGAAA+CGG - chr2.2:15074457-15074476 None:intergenic 45.0%
TGCAGCTTCTACCATATGAG+TGG + chr2.2:15074920-15074939 MS.gene059898:intron 45.0%
! ATTATCAAGCATCGCGACGA+AGG - chr2.2:15074743-15074762 None:intergenic 45.0%
! GAGTATACCTTGAGGTGCTT+CGG - chr2.2:15074490-15074509 None:intergenic 45.0%
!! GTTGCAATTCAGGCCTTGTT+TGG + chr2.2:15074882-15074901 MS.gene059898:intron 45.0%
!! TAAAAACGGTGCCGTTTCGA+TGG + chr2.2:15074443-15074462 MS.gene059898:CDS 45.0%
!!! GCATGCTTTTGCTCTGAAAC+AGG + chr2.2:15076553-15076572 MS.gene059898:intron 45.0%
!!! GCTTTTGCTCTGAAACAGGA+AGG + chr2.2:15076557-15076576 MS.gene059898:intron 45.0%
ATGGTGCGTCCAATATGTCG+TGG + chr2.2:15076401-15076420 MS.gene059898:intron 50.0%
GGAAGGTGAAGCAGAATGGA+AGG + chr2.2:15076574-15076593 MS.gene059898:intron 50.0%
TGCTTCGGAAACGATGGTGT+CGG - chr2.2:15074475-15074494 None:intergenic 50.0%
! ATCGTTTCCGAAGCACCTCA+AGG + chr2.2:15074480-15074499 MS.gene059898:CDS 50.0%
! TTGAGGTGCTTCGGAAACGA+TGG - chr2.2:15074481-15074500 None:intergenic 50.0%
!! ACTTGGTGCACTGCAAGATG+TGG + chr2.2:15075460-15075479 MS.gene059898:intron 50.0%
CGCGGCAGACTTCTCATTTG+CGG - chr2.2:15076655-15076674 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 15074411 15076737 15074411 ID=MS.gene059898
chr2.2 mRNA 15074411 15076737 15074411 ID=MS.gene059898.t1;Parent=MS.gene059898
chr2.2 exon 15074411 15074501 15074411 ID=MS.gene059898.t1.exon1;Parent=MS.gene059898.t1
chr2.2 CDS 15074411 15074501 15074411 ID=cds.MS.gene059898.t1;Parent=MS.gene059898.t1
chr2.2 exon 15074650 15074707 15074650 ID=MS.gene059898.t1.exon2;Parent=MS.gene059898.t1
chr2.2 CDS 15074650 15074707 15074650 ID=cds.MS.gene059898.t1;Parent=MS.gene059898.t1
chr2.2 exon 15076575 15076737 15076575 ID=MS.gene059898.t1.exon3;Parent=MS.gene059898.t1
chr2.2 CDS 15076575 15076737 15076575 ID=cds.MS.gene059898.t1;Parent=MS.gene059898.t1
Gene Sequence

>MS.gene059898

ATGGCGAAAAACAGGAACAAAAACAAGAAGAATAAAAACGGTGCCGTTTCGATGGACATAACCGACACCATCGTTTCCGAAGCACCTCAAGGTATACTCGCTTTTCAATTAACAACTAAAATTTCAATTTTTTTACTTTTATTTCTCTGCTTCTAATCTCATAGTGAACTCTTGTTTTGTTTTAATTTTCCTTTTTTTTTTTTGTGGTTTAAGATTTTGAGACTTTTGTTTATTTGCAGCAATGGATACATCAGAATCAGGAGCTAAAATCAAGGTTGCTGGTGGTGCTACCATTTTGTAAGTTTTCTTTATTGCCCTTATATTTTTAGCCTTCGTCGCGATGCTTGATAATGATATTGTAGAGAATCACAAGTTAAATGGGGTTTAGCGCGGTTGTGAGCTTGTTTTAAAACCCTAGTTTTTTAATGCTTTGGTTGATGGAACAGAATGATACCAACATTTTCTCAAATCGTTGCAATTCAGGCCTTGTTTGGATAAACAAATTAATTTGCAGCTTCTACCATATGAGTGGTTATGAATAAGCTATTTCTATTAACAATCTCACATGTGAATATGCTGGTAGGTAAGCTCAAATACGTTAATAATAGTTAATTCAGACATACCCTTAATCCAATAAGTTTGATTTGGTGCAACATAATTACCGTCCAGTTACTCAACTGATTATTGGATGTTGTTGTTGAATTGGGCATTGTCATCATGATGCCATATCTCCTCATATTCTAAGTTGGTATTTTAGAGTCGATGTAGTGAAGTTAGATCATGAGTCATCATTCTTTGTCGAACGGTTGAGATCACTTAACTGTGTGATATAGGAAATCTGGGCCCTCTTCCCTCCTATAATTGCGTTCAACTGTACACACGTTACTGCCATTTCTTCATTGTAGCAATGGCAATGTAGTTTTTTTTGCATGGACAAAACAACTGTATCTTCAATCACATTTACTTTTGCACTGGATGTTAGGTTCTAGACTTGGTTTTGATTACTTGATCTGTTGAGTTCTTGAAGAAGGGTATTCTTTAAAGCATGCACTTGGTGCACTGCAAGATGTGGTTTGTTAGTAATAGTAGTTGCCTTGTATTTTGGGTTTGCTTTGAACTTTATAAATACAAATTCGATGTAGTATTATAATTTTTTAAAGAAGGCGGTGAATATATCCTACGCACATATATGTTTTAAACATATTTTTTGTATTAAACAAACTCTTATGAGTCAAGTCTATAGACATTTAATGAGTCAAGTTTTATGAGACCCAGCAGAGCAGAAAACATTATCTTTCCATCACTAAATTGCCTGTGTAATTAGAATACGAACAATTCACTAATGGACAATGTTACCAATGCTGATCAATTTTGTAAGTGCAATATTTTGTTTAACAAAAATTTATATACTTCAGTTCAGTTCGCATAGTTGATGGATTGTGTACAATGCTTTTTCTGCATTGTCCCTCATTTTGATGACACTTTTTTTTTTTTTTTTTGAATACACTTGCTTTGTTTTGCTCTTCTGTTTTGTCATGGGCATGCCTATGAGTTATATGTTAACTGTTAGTTGTTGCCAATGTCCTCAATTATAACATGCGCTGGATCTGAAGTCCATCAGAATTTTTGCCTTGAATGATTGTTTGATAGAACAAGGCAACCCGATATCAATCGAGTCTATGATAGGAGAAAGCCAAATAGAGAGTTAAATAGGGGAAGTTATTTGTCATAACTGTCGATATTTATAACTGACAATAGTTAGCTAGGAAGAGTAGATGGATGGGAGTCTATCAATAGGAAAATTTGGTAGAGAGAAAATTTTGCATAATAATAGTTGTATGGGATATTTGTTATCAGGTTCCTCGAACCTCCTGTGAATTGTTCATCAGTTTTCAATAAATATTCTATACTTTCGCAGAGGAGATTTCCCTTCTGTAATATACTTCGCGAGTTCTAATGTGATTCAAGTTCCATCATTGTTTAAAACTTTATGGTGCGTCCAATATGTCGTGGAGTAAACACATATTACCTGCTTCCTGATTTCCATCTACTTGCAAAACATAATAATGCTCAAGTTCTTCTCATCTCGCACATTTAAAAGTTACTGTATGATAGTATTGCATTCTTACTGCATTTTCAAATGCATGCTTTTGCTCTGAAACAGGAAGGTGAAGCAGAATGGAAGGCCCATGAAGAGATCAAAAAGTGTTCGAAAGATGAAGAAAATAGCAAAAGCTATATCCGCAAATGAGAAGTCTGCCGCGAAAGTTTCCAAGTCTGAAAACAAAAAAAGTAGAGTTCAATCTGCGAAAACACTCTACGAATAA

Protein sequence

>MS.gene059898.t1

MAKNRNKNKKNKNGAVSMDITDTIVSEAPQAMDTSESGAKIKVAGGATILKVKQNGRPMKRSKSVRKMKKIAKAISANEKSAAKVSKSENKKSRVQSAKTLYE