AlfalfaGEDB | MS.gene33058

M. sativa cultivar XinJiangDaYe / MS.gene33058

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33058.t1	XP_003588905.1	97.4	39	0	1	1	39	24	61	3.40E-10	75.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33058.t1	G7I8Y6	97.4	39	0	1	1	39	24	61	2.5e-10	75.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049239	MS.gene33058	0.841222	5.42E-58	-1.69E-46
MS.gene051539	MS.gene33058	0.855921	4.56E-62	-1.69E-46
MS.gene055566	MS.gene33058	0.807572	4.74E-50	-1.69E-46
MS.gene059905	MS.gene33058	0.80955	1.79E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33058.t1	MTR_1g015040	86.175	217	28	2	1	216	24	239	1.96e-76	230

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 68 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTTCGCCTTCTTCGGGTTC+AGG	0.278379	1.1:+6677902	MS.gene33058:CDS
TGAGGCATAATAATAGTAAT+AGG	0.283637	1.1:-6677975	None:intergenic
TTTGAAATCTTCGCCTTCTT+CGG	0.306660	1.1:+6677895	MS.gene33058:CDS
GGTGGTCCACCAATTACTTT+AGG	0.326407	1.1:-6677935	None:intergenic
CAAGGACAACCTAAAGTAAT+TGG	0.337399	1.1:+6677926	MS.gene33058:CDS
TTGAAATCTTCGCCTTCTTC+GGG	0.338296	1.1:+6677896	MS.gene33058:CDS
GAATTTGATGAAGCAAGTTT+AGG	0.338870	1.1:-6677554	None:intergenic
AGTCCTTCGTCACATTCTTC+CGG	0.348481	1.1:+6677854	MS.gene33058:CDS
GTCCTTCGTCACATTCTTCC+GGG	0.349605	1.1:+6677855	MS.gene33058:CDS
TTTGATGATGTGAGTGGAAA+TGG	0.378894	1.1:-6677737	None:intergenic
TTCATCAGATATTCTTTGCT+TGG	0.384427	1.1:+6678053	MS.gene33058:CDS
TAATAATAGTAATAGGGATA+TGG	0.384788	1.1:-6677968	None:intergenic
TGTGTTAGGAGTGGAGTAGA+TGG	0.387212	1.1:-6677674	None:intergenic
TTTAGAAGTGAAGGAGGAGG+AGG	0.388578	1.1:-6677764	None:intergenic
GTTAATAGTGGAGGAGGTGG+AGG	0.430557	1.1:-6677701	None:intergenic
TGGGGTGTTATTAGTGGAAA+AGG	0.432885	1.1:-6677647	None:intergenic
TTGATGATGTGAGTGGAAAT+GGG	0.439050	1.1:-6677736	None:intergenic
CCTTCTTCGGGTTCAGGTCA+AGG	0.439335	1.1:+6677908	MS.gene33058:CDS
GGTGAATATGATGTTAATAG+TGG	0.445887	1.1:-6677713	None:intergenic
GAGGCATAATAATAGTAATA+GGG	0.446723	1.1:-6677974	None:intergenic
AGTGGAGTAGATGGAGGGTG+GGG	0.452281	1.1:-6677665	None:intergenic
GGAGGGTGGGGTGTTATTAG+TGG	0.452753	1.1:-6677653	None:intergenic
ATGCATAATAAACCACTTGA+AGG	0.460999	1.1:-6677488	None:intergenic
GGGGTGTTATTAGTGGAAAA+GGG	0.461571	1.1:-6677646	None:intergenic
GATGTTAATAGTGGAGGAGG+TGG	0.484497	1.1:-6677704	None:intergenic
GGGTGTTATTAGTGGAAAAG+GGG	0.496788	1.1:-6677645	None:intergenic
AAATAGAACATTGGAGATTG+AGG	0.504682	1.1:+6677448	MS.gene33058:CDS
GAGTGGAGTAGATGGAGGGT+GGG	0.505478	1.1:-6677666	None:intergenic
GAAGAATGTGACGAAGGACT+TGG	0.508990	1.1:-6677851	None:intergenic
GGCGAAGATTTCAAAGGTGG+TGG	0.511485	1.1:-6677887	None:intergenic
GGAGTGGAGTAGATGGAGGG+TGG	0.519473	1.1:-6677667	None:intergenic
GGTGGTGGTGAACAAATTGT+AGG	0.526245	1.1:-6677530	None:intergenic
GTCAACAAAGGAGGAGGTGA+AGG	0.526752	1.1:-6677611	None:intergenic
TTTGTTTGCAAATAGAACAT+TGG	0.528234	1.1:+6677439	MS.gene33058:CDS
TTTGATGAAGCAAGTTTAGG+AGG	0.539473	1.1:-6677551	None:intergenic
GATGAAGCAAGTTTAGGAGG+TGG	0.545014	1.1:-6677548	None:intergenic
GAAGATTTCAAAGGTGGTGG+AGG	0.554576	1.1:-6677884	None:intergenic
GAATGTGACGAAGGACTTGG+TGG	0.555897	1.1:-6677848	None:intergenic
CCTTGACCTGAACCCGAAGA+AGG	0.567300	1.1:-6677908	None:intergenic
TATGATGTTAATAGTGGAGG+AGG	0.568744	1.1:-6677707	None:intergenic
GGACAACCTAAAGTAATTGG+TGG	0.583322	1.1:+6677929	MS.gene33058:CDS
GAAGCAAGTTTAGGAGGTGG+TGG	0.584772	1.1:-6677545	None:intergenic
TGATGATGTGAGTGGAAATG+GGG	0.585770	1.1:-6677735	None:intergenic
AAAGTTGAAGAGCCTTCAAG+TGG	0.589794	1.1:+6677476	MS.gene33058:CDS
AGGTGGTGGAGGAGTTTCCC+CGG	0.592101	1.1:-6677873	None:intergenic
GTGGTGGAAGACTTAGTGAT+GGG	0.594811	1.1:-6677799	None:intergenic
GTTAGGAGTGGAGTAGATGG+AGG	0.597422	1.1:-6677671	None:intergenic
TTAGGAGTGGAGTAGATGGA+GGG	0.598597	1.1:-6677670	None:intergenic
TTTGGTGTCAACAAAGGAGG+AGG	0.602071	1.1:-6677617	None:intergenic
GGTGCATTTGGTGTCAACAA+AGG	0.602133	1.1:-6677623	None:intergenic
GAAGGACTTGGTGGAGATGG+TGG	0.605778	1.1:-6677839	None:intergenic
GGTGGAAGACTTAGTGATGG+GGG	0.608316	1.1:-6677797	None:intergenic
GACGAAGGACTTGGTGGAGA+TGG	0.610720	1.1:-6677842	None:intergenic
GAATATGATGTTAATAGTGG+AGG	0.611804	1.1:-6677710	None:intergenic
GGTAGAGTCAACAATGGTGG+TGG	0.613265	1.1:-6677815	None:intergenic
GGACTTGGTGGAGATGGTGG+CGG	0.623426	1.1:-6677836	None:intergenic
GGAGAATTTGATGATGTGAG+TGG	0.631413	1.1:-6677743	None:intergenic
GCATTTGGTGTCAACAAAGG+AGG	0.639121	1.1:-6677620	None:intergenic
TCCTTCGTCACATTCTTCCG+GGG	0.641768	1.1:+6677856	MS.gene33058:CDS
TGGTGGAAGACTTAGTGATG+GGG	0.649103	1.1:-6677798	None:intergenic
GAAGAAGGCGAAGATTTCAA+AGG	0.649156	1.1:-6677893	None:intergenic
GAAGGCGAAGATTTCAAAGG+TGG	0.657373	1.1:-6677890	None:intergenic
GGCGGTAGAGTCAACAATGG+TGG	0.660549	1.1:-6677818	None:intergenic
GGTGGTGGAAGACTTAGTGA+TGG	0.666350	1.1:-6677800	None:intergenic
GGTGTTATTAGTGGAAAAGG+GGG	0.675151	1.1:-6677644	None:intergenic
TCCCCGGAAGAATGTGACGA+AGG	0.679405	1.1:-6677857	None:intergenic
GATGATGTGAGTGGAAATGG+GGG	0.687997	1.1:-6677734	None:intergenic
GGTGGCGGTAGAGTCAACAA+TGG	0.730976	1.1:-6677821	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAATAATAGTAATAGGGATA+TGG	-	chr1.1:6677971-6677990	None:intergenic	20.0%
!	GAGGCATAATAATAGTAATA+GGG	-	chr1.1:6677977-6677996	None:intergenic	25.0%
!	TGAGGCATAATAATAGTAAT+AGG	-	chr1.1:6677978-6677997	None:intergenic	25.0%
!	TTTGTTTGCAAATAGAACAT+TGG	+	chr1.1:6677439-6677458	MS.gene33058:CDS	25.0%
	AAATAGAACATTGGAGATTG+AGG	+	chr1.1:6677448-6677467	MS.gene33058:CDS	30.0%
	ATGCATAATAAACCACTTGA+AGG	-	chr1.1:6677491-6677510	None:intergenic	30.0%
	GAATATGATGTTAATAGTGG+AGG	-	chr1.1:6677713-6677732	None:intergenic	30.0%
	GAATTTGATGAAGCAAGTTT+AGG	-	chr1.1:6677557-6677576	None:intergenic	30.0%
	GGTGAATATGATGTTAATAG+TGG	-	chr1.1:6677716-6677735	None:intergenic	30.0%
	TTCATCAGATATTCTTTGCT+TGG	+	chr1.1:6678053-6678072	MS.gene33058:CDS	30.0%
!!	GGATATGATTTTAGAAGTGA+AGG	-	chr1.1:6677776-6677795	None:intergenic	30.0%
!!	TATGATTTTAGAAGTGAAGG+AGG	-	chr1.1:6677773-6677792	None:intergenic	30.0%
	AGAAAAATGAGATGCACTTG+AGG	-	chr1.1:6677996-6678015	None:intergenic	35.0%
	CAAGGACAACCTAAAGTAAT+TGG	+	chr1.1:6677926-6677945	MS.gene33058:CDS	35.0%
	TTTGATGAAGCAAGTTTAGG+AGG	-	chr1.1:6677554-6677573	None:intergenic	35.0%
!	TATGATGTTAATAGTGGAGG+AGG	-	chr1.1:6677710-6677729	None:intergenic	35.0%
!	TTTGAAATCTTCGCCTTCTT+CGG	+	chr1.1:6677895-6677914	MS.gene33058:CDS	35.0%
!!	TAGGGATATGGATAGTTTTG+TGG	-	chr1.1:6677959-6677978	None:intergenic	35.0%
!!	TTGATGATGTGAGTGGAAAT+GGG	-	chr1.1:6677739-6677758	None:intergenic	35.0%
!!	TTTGATGATGTGAGTGGAAA+TGG	-	chr1.1:6677740-6677759	None:intergenic	35.0%
	AAAGTTGAAGAGCCTTCAAG+TGG	+	chr1.1:6677476-6677495	MS.gene33058:CDS	40.0%
	GAAGAAGGCGAAGATTTCAA+AGG	-	chr1.1:6677896-6677915	None:intergenic	40.0%
	GGACAACCTAAAGTAATTGG+TGG	+	chr1.1:6677929-6677948	MS.gene33058:CDS	40.0%
	TTGAAATCTTCGCCTTCTTC+GGG	+	chr1.1:6677896-6677915	MS.gene33058:CDS	40.0%
!	AGGTGAAGGATTTTGTGTTG+GGG	-	chr1.1:6677600-6677619	None:intergenic	40.0%
!	GAGGTGAAGGATTTTGTGTT+GGG	-	chr1.1:6677601-6677620	None:intergenic	40.0%
!	TGATGATGTGAGTGGAAATG+GGG	-	chr1.1:6677738-6677757	None:intergenic	40.0%
!!	GATTTTAGAAGTGAAGGAGG+AGG	-	chr1.1:6677770-6677789	None:intergenic	40.0%
!!	GGAGAATTTGATGATGTGAG+TGG	-	chr1.1:6677746-6677765	None:intergenic	40.0%
!!	GGGGTGTTATTAGTGGAAAA+GGG	-	chr1.1:6677649-6677668	None:intergenic	40.0%
!!	GGGTGTTATTAGTGGAAAAG+GGG	-	chr1.1:6677648-6677667	None:intergenic	40.0%
!!	GGTGTTATTAGTGGAAAAGG+GGG	-	chr1.1:6677647-6677666	None:intergenic	40.0%
!!	TGGGGTGTTATTAGTGGAAA+AGG	-	chr1.1:6677650-6677669	None:intergenic	40.0%
!!!	GGATATGGATAGTTTTGTGG+TGG	-	chr1.1:6677956-6677975	None:intergenic	40.0%
	AGTCCTTCGTCACATTCTTC+CGG	+	chr1.1:6677854-6677873	MS.gene33058:CDS	45.0%
	GAAGAATGTGACGAAGGACT+TGG	-	chr1.1:6677854-6677873	None:intergenic	45.0%
	GAAGGCGAAGATTTCAAAGG+TGG	-	chr1.1:6677893-6677912	None:intergenic	45.0%
	GATGAAGCAAGTTTAGGAGG+TGG	-	chr1.1:6677551-6677570	None:intergenic	45.0%
	GATGATGTGAGTGGAAATGG+GGG	-	chr1.1:6677737-6677756	None:intergenic	45.0%
	GGTGGTCCACCAATTACTTT+AGG	-	chr1.1:6677938-6677957	None:intergenic	45.0%
	TGGTGGAAGACTTAGTGATG+GGG	-	chr1.1:6677801-6677820	None:intergenic	45.0%
	TGTGTTAGGAGTGGAGTAGA+TGG	-	chr1.1:6677677-6677696	None:intergenic	45.0%
	TTAGGAGTGGAGTAGATGGA+GGG	-	chr1.1:6677673-6677692	None:intergenic	45.0%
	TTTAGAAGTGAAGGAGGAGG+AGG	-	chr1.1:6677767-6677786	None:intergenic	45.0%
!	GAAGATTTCAAAGGTGGTGG+AGG	-	chr1.1:6677887-6677906	None:intergenic	45.0%
!	GAGGTGGAGGATTTTGTGTT+AGG	-	chr1.1:6677691-6677710	None:intergenic	45.0%
!	GATGTTAATAGTGGAGGAGG+TGG	-	chr1.1:6677707-6677726	None:intergenic	45.0%
!	GGAGGATTTTGTGTTAGGAG+TGG	-	chr1.1:6677686-6677705	None:intergenic	45.0%
!	GGAGGTGAAGGATTTTGTGT+TGG	-	chr1.1:6677602-6677621	None:intergenic	45.0%
!	GGTGAAGGATTTTGTGTTGG+GGG	-	chr1.1:6677599-6677618	None:intergenic	45.0%
!	GGTGGTGGTGAACAAATTGT+AGG	-	chr1.1:6677533-6677552	None:intergenic	45.0%
!	GTGGTGGAAGACTTAGTGAT+GGG	-	chr1.1:6677802-6677821	None:intergenic	45.0%
!!	GCATTTGGTGTCAACAAAGG+AGG	-	chr1.1:6677623-6677642	None:intergenic	45.0%
!!	GGTGCATTTGGTGTCAACAA+AGG	-	chr1.1:6677626-6677645	None:intergenic	45.0%
!!	TTTGGTGTCAACAAAGGAGG+AGG	-	chr1.1:6677620-6677639	None:intergenic	45.0%
	GAAGCAAGTTTAGGAGGTGG+TGG	-	chr1.1:6677548-6677567	None:intergenic	50.0%
	GAATGTGACGAAGGACTTGG+TGG	-	chr1.1:6677851-6677870	None:intergenic	50.0%
	GGCGAAGATTTCAAAGGTGG+TGG	-	chr1.1:6677890-6677909	None:intergenic	50.0%
	GGTAGAGTCAACAATGGTGG+TGG	-	chr1.1:6677818-6677837	None:intergenic	50.0%
	GGTGGAAGACTTAGTGATGG+GGG	-	chr1.1:6677800-6677819	None:intergenic	50.0%
	GTCAACAAAGGAGGAGGTGA+AGG	-	chr1.1:6677614-6677633	None:intergenic	50.0%
	GTCCTTCGTCACATTCTTCC+GGG	+	chr1.1:6677855-6677874	MS.gene33058:CDS	50.0%
	GTTAGGAGTGGAGTAGATGG+AGG	-	chr1.1:6677674-6677693	None:intergenic	50.0%
	TCCTTCGTCACATTCTTCCG+GGG	+	chr1.1:6677856-6677875	MS.gene33058:CDS	50.0%
!	AGTGGAAAAGGGGGTGCATT+TGG	-	chr1.1:6677638-6677657	None:intergenic	50.0%
!	GGATTTTGTGTTGGGGGTGA+AGG	-	chr1.1:6677593-6677612	None:intergenic	50.0%
!	GTTAATAGTGGAGGAGGTGG+AGG	-	chr1.1:6677704-6677723	None:intergenic	50.0%
!	TTTTGTGTTGGGGGTGAAGG+AGG	-	chr1.1:6677590-6677609	None:intergenic	50.0%
!!	GGTGGTGGAAGACTTAGTGA+TGG	-	chr1.1:6677803-6677822	None:intergenic	50.0%
	AGTGGAGTAGATGGAGGGTG+GGG	-	chr1.1:6677668-6677687	None:intergenic	55.0%
	CCTTGACCTGAACCCGAAGA+AGG	-	chr1.1:6677911-6677930	None:intergenic	55.0%
	GAGTGGAGTAGATGGAGGGT+GGG	-	chr1.1:6677669-6677688	None:intergenic	55.0%
	GGCGGTAGAGTCAACAATGG+TGG	-	chr1.1:6677821-6677840	None:intergenic	55.0%
	TCCCCGGAAGAATGTGACGA+AGG	-	chr1.1:6677860-6677879	None:intergenic	55.0%
!	CCTTCTTCGGGTTCAGGTCA+AGG	+	chr1.1:6677908-6677927	MS.gene33058:CDS	55.0%
!	GGAAGACTTAGTGATGGGGG+AGG	-	chr1.1:6677797-6677816	None:intergenic	55.0%
!	GGTGGCGGTAGAGTCAACAA+TGG	-	chr1.1:6677824-6677843	None:intergenic	55.0%
!	TCTTCGCCTTCTTCGGGTTC+AGG	+	chr1.1:6677902-6677921	MS.gene33058:CDS	55.0%
!!	GAAGGACTTGGTGGAGATGG+TGG	-	chr1.1:6677842-6677861	None:intergenic	55.0%
!!	GACGAAGGACTTGGTGGAGA+TGG	-	chr1.1:6677845-6677864	None:intergenic	55.0%
!!	GGAGGGTGGGGTGTTATTAG+TGG	-	chr1.1:6677656-6677675	None:intergenic	55.0%
	GGAGTGGAGTAGATGGAGGG+TGG	-	chr1.1:6677670-6677689	None:intergenic	60.0%
	GTGTTGGGGGTGAAGGAGGA+GGG	-	chr1.1:6677586-6677605	None:intergenic	60.0%
	TGTGTTGGGGGTGAAGGAGG+AGG	-	chr1.1:6677587-6677606	None:intergenic	60.0%
!!	AGGTGGTGGAGGAGTTTCCC+CGG	-	chr1.1:6677876-6677895	None:intergenic	60.0%
!!	GGACTTGGTGGAGATGGTGG+CGG	-	chr1.1:6677839-6677858	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	6677428	6678078	6677428	ID=MS.gene33058
chr1.1	mRNA	6677428	6678078	6677428	ID=MS.gene33058.t1;Parent=MS.gene33058
chr1.1	exon	6677428	6678078	6677428	ID=MS.gene33058.t1.exon1;Parent=MS.gene33058.t1
chr1.1	CDS	6677428	6678078	6677428	ID=cds.MS.gene33058.t1;Parent=MS.gene33058.t1

Gene Sequence

>MS.gene33058

ATGTTGTTGGTTTTGTTTGCAAATAGAACATTGGAGATTGAGGACAACAAAGTTGAAGAGCCTTCAAGTGGTTTATTATGCATTAGTGAATGTGTCACATGTCCTACAATTTGTTCACCACCACCTCCTAAACTTGCTTCATCAAATTCAAATCACCCTCCTCCTTCACCCCCAACACAAAATCCTTCACCTCCTCCTTTGTTGACACCAAATGCACCCCCTTTTCCACTAATAACACCCCACCCTCCATCTACTCCACTCCTAACACAAAATCCTCCACCTCCTCCACTATTAACATCATATTCACCCCCATTTCCACTCACATCATCAAATTCTCCTCCTCCTCCTTCACTTCTAAAATCATATCCTCCCCCATCACTAAGTCTTCCACCACCATTGTTGACTCTACCGCCACCATCTCCACCAAGTCCTTCGTCACATTCTTCCGGGGAAACTCCTCCACCACCTTTGAAATCTTCGCCTTCTTCGGGTTCAGGTCAAGGACAACCTAAAGTAATTGGTGGACCACCACAAAACTATCCATATCCCTATTACTATTATTATGCCTCAAGTGCATCTCATTTTTCTAATCATGTTGTACTCTTCTTTATTGTACTTTTGTTCATTCATCAGATATTCTTTGCTTGGTAA

Protein sequence

>MS.gene33058.t1

MLLVLFANRTLEIEDNKVEEPSSGLLCISECVTCPTICSPPPPKLASSNSNHPPPSPPTQNPSPPPLLTPNAPPFPLITPHPPSTPLLTQNPPPPPLLTSYSPPFPLTSSNSPPPPSLLKSYPPPSLSLPPPLLTLPPPSPPSPSSHSSGETPPPPLKSSPSSGSGQGQPKVIGGPPQNYPYPYYYYYASSASHFSNHVVLFFIVLLFIHQIFFAW