Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055566.t1 | AES61347.1 | 94 | 117 | 7 | 0 | 1 | 117 | 1 | 117 | 7.20E-58 | 233 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055566.t1 | Q84MC0 | 36.4 | 107 | 66 | 2 | 10 | 116 | 6 | 110 | 1.3e-10 | 67.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055566.t1 | G7I826 | 94.0 | 117 | 7 | 0 | 1 | 117 | 1 | 117 | 5.2e-58 | 233.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049239 | MS.gene055566 | 0.837578 | 4.78E-57 | -1.69E-46 |
| MS.gene055566 | MS.gene06362 | 0.837208 | 5.95E-57 | -1.69E-46 |
| MS.gene055566 | MS.gene31804 | 0.805846 | 1.10E-49 | -1.69E-46 |
| MS.gene055566 | MS.gene32359 | 0.840274 | 9.60E-58 | -1.69E-46 |
| MS.gene055566 | MS.gene33058 | 0.807572 | 4.74E-50 | -1.69E-46 |
| MS.gene055566 | MS.gene38102 | 0.805817 | 1.11E-49 | -1.69E-46 |
| MS.gene055566 | MS.gene40936 | 0.82722 | 1.76E-54 | -1.69E-46 |
| MS.gene055566 | MS.gene49981 | 0.807567 | 4.75E-50 | -1.69E-46 |
| MS.gene055566 | MS.gene58539 | 0.812493 | 4.12E-51 | -1.69E-46 |
| MS.gene055566 | MS.gene62740 | 0.801171 | 1.02E-48 | -1.69E-46 |
| MS.gene055566 | MS.gene74253 | 0.829547 | 4.84E-55 | -1.69E-46 |
| MS.gene055566 | MS.gene90899 | 0.833991 | 3.88E-56 | -1.69E-46 |
| MS.gene055566 | MS.gene91390 | 0.819203 | 1.31E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055566.t1 | MTR_1g082770 | 94.017 | 117 | 7 | 0 | 1 | 117 | 1 | 117 | 3.60e-69 | 206 |
| MS.gene055566.t1 | MTR_1g082790 | 61.240 | 129 | 35 | 2 | 1 | 115 | 1 | 128 | 3.77e-41 | 134 |
| MS.gene055566.t1 | MTR_1g082830 | 67.442 | 86 | 27 | 1 | 32 | 117 | 47 | 131 | 1.54e-37 | 126 |
| MS.gene055566.t1 | MTR_7g113640 | 46.591 | 88 | 46 | 1 | 31 | 117 | 27 | 114 | 6.37e-22 | 85.9 |
| MS.gene055566.t1 | MTR_5g005710 | 35.185 | 108 | 68 | 2 | 10 | 116 | 4 | 110 | 1.59e-16 | 72.0 |
| MS.gene055566.t1 | MTR_1g105305 | 33.645 | 107 | 69 | 2 | 10 | 116 | 10 | 114 | 1.98e-14 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055566.t1 | AT1G54860 | 31.579 | 114 | 74 | 2 | 5 | 117 | 6 | 116 | 3.18e-15 | 68.9 |
| MS.gene055566.t1 | AT3G06035 | 36.471 | 85 | 53 | 1 | 32 | 116 | 27 | 110 | 1.73e-14 | 66.6 |
| MS.gene055566.t1 | AT5G19240 | 33.721 | 86 | 56 | 1 | 32 | 117 | 28 | 112 | 8.04e-13 | 62.4 |
| MS.gene055566.t1 | AT5G19250 | 34.884 | 86 | 55 | 1 | 31 | 116 | 27 | 111 | 2.31e-12 | 61.2 |
Find 24 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACATCAATACCACAAAAGA+TGG | 0.409765 | 1.2:-60092657 | MS.gene055566:CDS |
| TCCTCCTTCAAACATGGTCT+TGG | 0.411097 | 1.2:-60093438 | MS.gene055566:CDS |
| TTGGAGCTTGGAAGGGTCAA+TGG | 0.458642 | 1.2:-60092434 | MS.gene055566:intron |
| TTTCGAAGTTAGGAATTGTC+GGG | 0.464042 | 1.2:+60092703 | None:intergenic |
| CTAACTTCGAAAAGAGTGTT+GGG | 0.482345 | 1.2:-60092692 | MS.gene055566:CDS |
| CACAAGTCACCGGACTTGTA+AGG | 0.482998 | 1.2:+60093379 | None:intergenic |
| CCTAACTTCGAAAAGAGTGT+TGG | 0.496092 | 1.2:-60092693 | MS.gene055566:CDS |
| AGCTAAACATGACGACTTGT+CGG | 0.502817 | 1.2:+60092803 | None:intergenic |
| CCACAACCTCCTTACAAGTC+CGG | 0.504950 | 1.2:-60093388 | MS.gene055566:CDS |
| GGTAAAAGACAAGTAGAAGA+AGG | 0.505225 | 1.2:+60093409 | None:intergenic |
| ACATCAATACCACAAAAGAT+GGG | 0.526345 | 1.2:-60092656 | MS.gene055566:CDS |
| CCGGACTTGTAAGGAGGTTG+TGG | 0.527323 | 1.2:+60093388 | None:intergenic |
| CCTCCGCAGGCACAAGTCAC+CGG | 0.536069 | 1.2:+60093369 | None:intergenic |
| GAGACCAAGACCATGTTTGA+AGG | 0.551223 | 1.2:+60093434 | None:intergenic |
| AACTATTACCCTAATATACC+TGG | 0.564594 | 1.2:-60092732 | MS.gene055566:CDS |
| TTTCCATGCAGAGAAGGAGA+AGG | 0.579150 | 1.2:-60092886 | MS.gene055566:intron |
| ACCATGTTTGAAGGAGGACA+TGG | 0.614142 | 1.2:+60093443 | None:intergenic |
| AGTCCGGTGACTTGTGCCTG+CGG | 0.619898 | 1.2:-60093372 | MS.gene055566:CDS |
| CTGTAGTGTATTACCTCCGC+AGG | 0.628973 | 1.2:+60093356 | None:intergenic |
| ACCATGTCCTCCTTCAAACA+TGG | 0.632031 | 1.2:-60093444 | MS.gene055566:CDS |
| CCGGTGACTTGTGCCTGCGG+AGG | 0.640108 | 1.2:-60093369 | MS.gene055566:intron |
| ACCAAGACCATGTTTGAAGG+AGG | 0.654950 | 1.2:+60093437 | None:intergenic |
| AAGTCACCGGACTTGTAAGG+AGG | 0.688883 | 1.2:+60093382 | None:intergenic |
| CATCAATACCACAAAAGATG+GGG | 0.692507 | 1.2:-60092655 | MS.gene055566:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAATATAACTTTTAAAATTG+CGG | + | chr1.2:60092659-60092678 | None:intergenic | 10.0% |
| !!! | TAGAGTTTATATTATTTTTT+TGG | + | chr1.2:60092939-60092958 | None:intergenic | 10.0% |
| !!! | ATATAAGCTAGTTTATTTTT+TGG | + | chr1.2:60092887-60092906 | None:intergenic | 15.0% |
| !!! | TTGCTATTTTGTAAATTTAA+AGG | + | chr1.2:60092571-60092590 | None:intergenic | 15.0% |
| !!! | TTTAAGAAACTTTTACTATT+TGG | - | chr1.2:60092802-60092821 | MS.gene055566:CDS | 15.0% |
| !!! | TTTATATTATTTTTTTGGAG+AGG | + | chr1.2:60092934-60092953 | None:intergenic | 15.0% |
| !! | AATTAGAAAACAAATCATCT+TGG | + | chr1.2:60093270-60093289 | None:intergenic | 20.0% |
| !! | AATTCAAAGTATATTATAGC+AGG | - | chr1.2:60093325-60093344 | MS.gene055566:intron | 20.0% |
| !! | ATTCAAAGTATATTATAGCA+GGG | - | chr1.2:60093326-60093345 | MS.gene055566:intron | 20.0% |
| !! | TCTCTTAAATTAAACAATGT+TGG | - | chr1.2:60092596-60092615 | MS.gene055566:intron | 20.0% |
| ! | ACTAAAGAGATATATATCGA+TGG | - | chr1.2:60092752-60092771 | MS.gene055566:CDS | 25.0% |
| ! | GATAGATGTTTGTAATTTCT+AGG | - | chr1.2:60092845-60092864 | MS.gene055566:CDS | 25.0% |
| ! | TTCAAAGTATATTATAGCAG+GGG | - | chr1.2:60093327-60093346 | MS.gene055566:intron | 25.0% |
| AAATCATCTTGGTCCAATTT+AGG | + | chr1.2:60093259-60093278 | None:intergenic | 30.0% | |
| AACATCAATACCACAAAAGA+TGG | - | chr1.2:60093211-60093230 | MS.gene055566:intron | 30.0% | |
| AACTATTACCCTAATATACC+TGG | - | chr1.2:60093136-60093155 | MS.gene055566:intron | 30.0% | |
| ACATCAATACCACAAAAGAT+GGG | - | chr1.2:60093212-60093231 | MS.gene055566:intron | 30.0% | |
| ATGGAACGACTAAATTGATA+AGG | - | chr1.2:60092771-60092790 | MS.gene055566:CDS | 30.0% | |
| ! | GGTTTTTACCAGGTATATTA+GGG | + | chr1.2:60093147-60093166 | None:intergenic | 30.0% |
| ! | TTTTCGAAGTTAGGAATTGT+CGG | + | chr1.2:60093169-60093188 | None:intergenic | 30.0% |
| AAGTATATTATAGCAGGGGT+TGG | - | chr1.2:60093331-60093350 | MS.gene055566:intron | 35.0% | |
| ATTAGCACTAACACAACTTC+TGG | - | chr1.2:60093382-60093401 | MS.gene055566:CDS | 35.0% | |
| CATCAATACCACAAAAGATG+GGG | - | chr1.2:60093213-60093232 | MS.gene055566:intron | 35.0% | |
| GGTAAAAGACAAGTAGAAGA+AGG | + | chr1.2:60092462-60092481 | None:intergenic | 35.0% | |
| TCTGCTAATTCTTTGCTTGT+TGG | - | chr1.2:60093415-60093434 | MS.gene055566:CDS | 35.0% | |
| TTTCGAAGTTAGGAATTGTC+GGG | + | chr1.2:60093168-60093187 | None:intergenic | 35.0% | |
| ! | ATCTATTTTCCATGCAGAGA+AGG | - | chr1.2:60092976-60092995 | MS.gene055566:intron | 35.0% |
| ! | GGGTTTTTACCAGGTATATT+AGG | + | chr1.2:60093148-60093167 | None:intergenic | 35.0% |
| !! | CTAACTTCGAAAAGAGTGTT+GGG | - | chr1.2:60093176-60093195 | MS.gene055566:intron | 35.0% |
| AGCTAAACATGACGACTTGT+CGG | + | chr1.2:60093068-60093087 | None:intergenic | 40.0% | |
| TCCAATTTAGGCACACACAT+TGG | + | chr1.2:60093247-60093266 | None:intergenic | 40.0% | |
| ! | ATTCTTTGCTTGTTGGAGCT+TGG | - | chr1.2:60093422-60093441 | MS.gene055566:CDS | 40.0% |
| ! | CATAATGACCCCATCTTTTG+TGG | + | chr1.2:60093224-60093243 | None:intergenic | 40.0% |
| ! | CCAACACTCTTTTCGAAGTT+AGG | + | chr1.2:60093178-60093197 | None:intergenic | 40.0% |
| ! | CCTAACTTCGAAAAGAGTGT+TGG | - | chr1.2:60093175-60093194 | MS.gene055566:intron | 40.0% |
| !! | TGGTTCTGAAGATGATTGGA+TGG | - | chr1.2:60093351-60093370 | MS.gene055566:intron | 40.0% |
| ACCAAGACCATGTTTGAAGG+AGG | + | chr1.2:60092434-60092453 | None:intergenic | 45.0% | |
| GAGACCAAGACCATGTTTGA+AGG | + | chr1.2:60092437-60092456 | None:intergenic | 45.0% | |
| GCCAATGTGTGTGCCTAAAT+TGG | - | chr1.2:60093243-60093262 | MS.gene055566:intron | 45.0% | |
| TCCTCCTTCAAACATGGTCT+TGG | - | chr1.2:60092430-60092449 | MS.gene055566:intron | 45.0% | |
| TTTCCATGCAGAGAAGGAGA+AGG | - | chr1.2:60092982-60093001 | MS.gene055566:intron | 45.0% | |
| ! | GGAATTGTCGGGTTTTTACC+AGG | + | chr1.2:60093157-60093176 | None:intergenic | 45.0% |
| ! | TTGCTTGTTGGAGCTTGGAA+GGG | - | chr1.2:60093427-60093446 | MS.gene055566:CDS | 45.0% |
| ! | TTTGCTTGTTGGAGCTTGGA+AGG | - | chr1.2:60093426-60093445 | MS.gene055566:CDS | 45.0% |
| !! | GGGTTGGTTCTGAAGATGAT+TGG | - | chr1.2:60093347-60093366 | MS.gene055566:intron | 45.0% |
| !!! | ACGACTTGTCGGTTTTCTCT+AGG | + | chr1.2:60093057-60093076 | None:intergenic | 45.0% |
| !!! | TTGTCGGTTTTCTCTAGGAC+AGG | + | chr1.2:60093052-60093071 | None:intergenic | 45.0% |
| AAGTCACCGGACTTGTAAGG+AGG | + | chr1.2:60092489-60092508 | None:intergenic | 50.0% | |
| CACAAGTCACCGGACTTGTA+AGG | + | chr1.2:60092492-60092511 | None:intergenic | 50.0% | |
| CCACAACCTCCTTACAAGTC+CGG | - | chr1.2:60092480-60092499 | MS.gene055566:intron | 50.0% | |
| CTGTAGTGTATTACCTCCGC+AGG | + | chr1.2:60092515-60092534 | None:intergenic | 50.0% | |
| GATCCTTCTCCTTCTCTGCA+TGG | + | chr1.2:60092988-60093007 | None:intergenic | 50.0% | |
| TTGGAGCTTGGAAGGGTCAA+TGG | - | chr1.2:60093434-60093453 | MS.gene055566:CDS | 50.0% | |
| !!! | CGGTTTTCTCTAGGACAGGA+AGG | + | chr1.2:60093048-60093067 | None:intergenic | 50.0% |
| CCGGACTTGTAAGGAGGTTG+TGG | + | chr1.2:60092483-60092502 | None:intergenic | 55.0% | |
| ! | AGTCCGGTGACTTGTGCCTG+CGG | - | chr1.2:60092496-60092515 | MS.gene055566:intron | 60.0% |
| CCTCCGCAGGCACAAGTCAC+CGG | + | chr1.2:60092502-60092521 | None:intergenic | 65.0% | |
| ! | CCGGTGACTTGTGCCTGCGG+AGG | - | chr1.2:60092499-60092518 | MS.gene055566:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 60092427 | 60093463 | 60092427 | ID=MS.gene055566 |
| chr1.2 | mRNA | 60092427 | 60093463 | 60092427 | ID=MS.gene055566.t1;Parent=MS.gene055566 |
| chr1.2 | exon | 60093370 | 60093463 | 60093370 | ID=MS.gene055566.t1.exon1;Parent=MS.gene055566.t1 |
| chr1.2 | CDS | 60093370 | 60093463 | 60093370 | ID=cds.MS.gene055566.t1;Parent=MS.gene055566.t1 |
| chr1.2 | exon | 60092642 | 60092897 | 60092642 | ID=MS.gene055566.t1.exon2;Parent=MS.gene055566.t1 |
| chr1.2 | CDS | 60092642 | 60092897 | 60092642 | ID=cds.MS.gene055566.t1;Parent=MS.gene055566.t1 |
| chr1.2 | exon | 60092427 | 60092442 | 60092427 | ID=MS.gene055566.t1.exon3;Parent=MS.gene055566.t1 |
| chr1.2 | CDS | 60092427 | 60092442 | 60092427 | ID=cds.MS.gene055566.t1;Parent=MS.gene055566.t1 |
| Gene Sequence |
| Protein sequence |
