AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene33061


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33061.t1 MTR_1g015000 95.105 143 6 1 1 143 1 142 3.65e-98 278
MS.gene33061.t1 MTR_3g105330 59.060 149 49 4 1 143 1 143 4.50e-55 169
MS.gene33061.t1 MTR_4g019870 31.034 145 71 4 2 138 13 136 3.52e-18 75.9
MS.gene33061.t1 MTR_7g066070 32.283 127 65 2 2 128 22 127 1.12e-15 69.3
MS.gene33061.t1 MTR_0027s0220 30.000 140 76 4 2 139 27 146 6.13e-15 67.8
MS.gene33061.t1 MTR_4g065660 30.645 124 64 3 5 128 35 136 3.93e-14 65.5
MS.gene33061.t1 MTR_4g065123 28.148 135 75 2 1 135 27 139 1.08e-13 64.3
MS.gene33061.t1 MTR_4g064963 31.579 133 66 4 5 133 46 157 1.49e-13 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33061.t1 AT5G20650 55.102 147 61 3 1 143 1 146 1.18e-50 158
MS.gene33061.t1 AT2G26975 33.577 137 64 3 1 131 24 139 3.99e-20 80.9
MS.gene33061.t1 AT5G59040 34.375 128 63 2 1 128 29 135 2.94e-17 73.6
MS.gene33061.t1 AT5G59030 27.778 144 80 3 5 142 45 170 1.42e-13 64.7
MS.gene33061.t1 AT2G37925 30.469 128 71 2 1 128 29 138 1.45e-13 63.9
MS.gene33061.t1 AT3G46900 26.761 142 78 4 7 142 35 156 4.28e-13 63.2

Find 31 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAACATTCTACCAATATT+TGG 0.138215 1.1:-6696922 MS.gene33061:CDS
GAGATTCGGACTCCTCTTCT+TGG 0.249119 1.1:-6696837 MS.gene33061:CDS
ACCAATATTTGGAAAATATT+CGG 0.254322 1.1:-6696911 MS.gene33061:CDS
TCCTCTTCTTGGGTTGAATA+AGG 0.262656 1.1:-6696826 MS.gene33061:CDS
TGACACTCTTATTTGATTCA+TGG 0.286816 1.1:-6696998 MS.gene33061:CDS
TGATGCACATGACATTATAT+TGG 0.315744 1.1:-6697031 MS.gene33061:CDS
GATGCACATGACATTATATT+GGG 0.333053 1.1:-6697030 MS.gene33061:CDS
CGTTTGGTTACTTCTTGTTT+AGG 0.336421 1.1:-6696680 MS.gene33061:CDS
ATTCATGGAAAACCAATTCA+TGG 0.342364 1.1:-6696983 MS.gene33061:CDS
TTGGCTATAATGTCGTTTAA+TGG 0.343696 1.1:-6696735 MS.gene33061:CDS
TTTGGTGTGAATTCTGCTAT+TGG 0.364233 1.1:-6696771 MS.gene33061:CDS
ATTGTGGTTGGTTTAACGTT+TGG 0.388729 1.1:-6696696 MS.gene33061:CDS
TAGATAACTCGTCCATGAAT+TGG 0.393378 1.1:+6696971 None:intergenic
AGATTCGGACTCCTCTTCTT+GGG 0.397569 1.1:-6696836 MS.gene33061:CDS
TTGGTGTGAATTCTGCTATT+GGG 0.404245 1.1:-6696770 MS.gene33061:CDS
ATTTGGAAAATATTCGGATT+CGG 0.414816 1.1:-6696905 MS.gene33061:CDS
AGTCGCCGGTGCCGGAGATT+CGG 0.431491 1.1:-6696851 MS.gene33061:CDS
TATTGGGTATTTGTTGATGT+TGG 0.437573 1.1:-6696754 MS.gene33061:CDS
GAGGAACAAGGTTGTTGAAT+CGG 0.457237 1.1:-6696799 MS.gene33061:CDS
TTGGGTTGAATAAGGTTTCG+AGG 0.486516 1.1:-6696818 MS.gene33061:CDS
AGAAGAGGAGTCCGAATCTC+CGG 0.488903 1.1:+6696840 None:intergenic
CGAGGGGAGGACGCAGTCGC+CGG 0.496710 1.1:-6696865 MS.gene33061:CDS
GAGGACGCAGTCGCCGGTGC+CGG 0.509370 1.1:-6696859 MS.gene33061:CDS
ACCTTATTCAACCCAAGAAG+AGG 0.545808 1.1:+6696825 None:intergenic
GGAGTCCGAATCTCCGGCAC+CGG 0.550760 1.1:+6696846 None:intergenic
TCAGAGTGAAGAAGAGGCTG+CGG 0.552122 1.1:-6696655 MS.gene33061:CDS
TAGGAGTCAGAGTGAAGAAG+AGG 0.579273 1.1:-6696661 MS.gene33061:CDS
CACAAACTAAGCACATGCAC+AGG 0.579857 1.1:+6696617 None:intergenic
GAATAAGGTTTCGAGGAACA+AGG 0.583225 1.1:-6696811 MS.gene33061:CDS
GCTATAATGTCGTTTAATGG+TGG 0.610087 1.1:-6696732 MS.gene33061:CDS
ATGCACATGACATTATATTG+GGG 0.668922 1.1:-6697029 MS.gene33061:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACCAATATTTGGAAAATATT+CGG - chr1.1:6696744-6696763 MS.gene33061:CDS 20.0%
! ATTTGGAAAATATTCGGATT+CGG - chr1.1:6696750-6696769 MS.gene33061:CDS 25.0%
!! TCCGAATATTTTCCAAATAT+TGG + chr1.1:6696748-6696767 None:intergenic 25.0%
ATGCACATGACATTATATTG+GGG - chr1.1:6696626-6696645 MS.gene33061:CDS 30.0%
ATTCATGGAAAACCAATTCA+TGG - chr1.1:6696672-6696691 MS.gene33061:CDS 30.0%
GATGCACATGACATTATATT+GGG - chr1.1:6696625-6696644 MS.gene33061:CDS 30.0%
TGATGCACATGACATTATAT+TGG - chr1.1:6696624-6696643 MS.gene33061:CDS 30.0%
TGCAACATTCTACCAATATT+TGG - chr1.1:6696733-6696752 MS.gene33061:CDS 30.0%
! TATTGGGTATTTGTTGATGT+TGG - chr1.1:6696901-6696920 MS.gene33061:CDS 30.0%
! TGACACTCTTATTTGATTCA+TGG - chr1.1:6696657-6696676 MS.gene33061:CDS 30.0%
! TTGGCTATAATGTCGTTTAA+TGG - chr1.1:6696920-6696939 MS.gene33061:CDS 30.0%
!!! GTTGTTGAATCGGTTTTATT+TGG - chr1.1:6696866-6696885 MS.gene33061:CDS 30.0%
TAGATAACTCGTCCATGAAT+TGG + chr1.1:6696687-6696706 None:intergenic 35.0%
TTGGTGTGAATTCTGCTATT+GGG - chr1.1:6696885-6696904 MS.gene33061:CDS 35.0%
! ATTCGGTTGAAGTTTTTCTC+TGG - chr1.1:6696767-6696786 MS.gene33061:CDS 35.0%
! CGTTTGGTTACTTCTTGTTT+AGG - chr1.1:6696975-6696994 MS.gene33061:CDS 35.0%
! GCTATAATGTCGTTTAATGG+TGG - chr1.1:6696923-6696942 MS.gene33061:CDS 35.0%
! TTTGGTGTGAATTCTGCTAT+TGG - chr1.1:6696884-6696903 MS.gene33061:CDS 35.0%
!! ATTGTGGTTGGTTTAACGTT+TGG - chr1.1:6696959-6696978 MS.gene33061:CDS 35.0%
!! GTTTAATGGTGGTGTTTTTG+TGG - chr1.1:6696934-6696953 MS.gene33061:CDS 35.0%
ACCTTATTCAACCCAAGAAG+AGG + chr1.1:6696833-6696852 None:intergenic 40.0%
GAATAAGGTTTCGAGGAACA+AGG - chr1.1:6696844-6696863 MS.gene33061:CDS 40.0%
GAGGAACAAGGTTGTTGAAT+CGG - chr1.1:6696856-6696875 MS.gene33061:CDS 40.0%
! GTTTTTGTGGCTATTGTGGT+TGG - chr1.1:6696947-6696966 MS.gene33061:CDS 40.0%
! TTGAAGTTTTTCTCTGGCGA+GGG - chr1.1:6696773-6696792 MS.gene33061:CDS 40.0%
!! TCCTCTTCTTGGGTTGAATA+AGG - chr1.1:6696829-6696848 MS.gene33061:CDS 40.0%
!! TGGTGTTTTTGTGGCTATTG+TGG - chr1.1:6696943-6696962 MS.gene33061:CDS 40.0%
!! TTGGGTTGAATAAGGTTTCG+AGG - chr1.1:6696837-6696856 MS.gene33061:CDS 40.0%
AGATTCGGACTCCTCTTCTT+GGG - chr1.1:6696819-6696838 MS.gene33061:CDS 45.0%
TAGGAGTCAGAGTGAAGAAG+AGG - chr1.1:6696994-6697013 MS.gene33061:CDS 45.0%
! GTTGAAGTTTTTCTCTGGCG+AGG - chr1.1:6696772-6696791 MS.gene33061:CDS 45.0%
! TGAAGTTTTTCTCTGGCGAG+GGG - chr1.1:6696774-6696793 MS.gene33061:CDS 45.0%
AGAAGAGGAGTCCGAATCTC+CGG + chr1.1:6696818-6696837 None:intergenic 50.0%
GAGATTCGGACTCCTCTTCT+TGG - chr1.1:6696818-6696837 MS.gene33061:CDS 50.0%
TCAGAGTGAAGAAGAGGCTG+CGG - chr1.1:6697000-6697019 MS.gene33061:CDS 50.0%
! AGTTTTTCTCTGGCGAGGGG+AGG - chr1.1:6696777-6696796 MS.gene33061:CDS 55.0%
GGAGTCCGAATCTCCGGCAC+CGG + chr1.1:6696812-6696831 None:intergenic 65.0%
! AGTCGCCGGTGCCGGAGATT+CGG - chr1.1:6696804-6696823 MS.gene33061:CDS 65.0%
CGAGGGGAGGACGCAGTCGC+CGG - chr1.1:6696790-6696809 MS.gene33061:CDS 75.0%
GAGGACGCAGTCGCCGGTGC+CGG - chr1.1:6696796-6696815 MS.gene33061:CDS 75.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 6696623 6697054 6696623 ID=MS.gene33061
chr1.1 mRNA 6696623 6697054 6696623 ID=MS.gene33061.t1;Parent=MS.gene33061
chr1.1 exon 6696623 6697054 6696623 ID=MS.gene33061.t1.exon1;Parent=MS.gene33061.t1
chr1.1 CDS 6696623 6697054 6696623 ID=cds.MS.gene33061.t1;Parent=MS.gene33061.t1
Gene Sequence

>MS.gene33061

ATGATGCACATGACATTATATTGGGGAAAAAACGTGACACTCTTATTTGATTCATGGAAAACCAATTCATGGACGAGTTATCTACTGAGTTTAATTGCATGTTTCATGATTGCAACATTCTACCAATATTTGGAAAATATTCGGATTCGGTTGAAGTTTTTCTCTGGCGAGGGGAGGACGCAGTCGCCGGTGCCGGAGATTCGGACTCCTCTTCTTGGGTTGAATAAGGTTTCGAGGAACAAGGTTGTTGAATCGGTTTTATTTGGTGTGAATTCTGCTATTGGGTATTTGTTGATGTTGGCTATAATGTCGTTTAATGGTGGTGTTTTTGTGGCTATTGTGGTTGGTTTAACGTTTGGTTACTTCTTGTTTAGGAGTCAGAGTGAAGAAGAGGCTGCGGTTGTTGTTGATAATTCCTGTGCATGTGCTTAG

Protein sequence

>MS.gene33061.t1

MMHMTLYWGKNVTLLFDSWKTNSWTSYLLSLIACFMIATFYQYLENIRIRLKFFSGEGRTQSPVPEIRTPLLGLNKVSRNKVVESVLFGVNSAIGYLLMLAIMSFNGGVFVAIVVGLTFGYFLFRSQSEEEAAVVVDNSCACA