Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33235.t1 | PNX75835.1 | 63.6 | 44 | 16 | 0 | 1 | 44 | 1 | 44 | 1.30E-06 | 62.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33235.t1 | A0A2K3LBB7 | 63.6 | 44 | 16 | 0 | 1 | 44 | 1 | 44 | 9.7e-07 | 62.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057406 | MS.gene33235 | 0.814553 | 1.45E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33235.t1 | MTR_2g044740 | 51.020 | 98 | 44 | 2 | 1 | 98 | 1 | 94 | 2.60e-14 | 63.9 |
| MS.gene33235.t1 | MTR_2g042470 | 36.893 | 103 | 56 | 2 | 1 | 99 | 1 | 98 | 6.73e-12 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 35 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATACATTGTTTCCCATATC+TGG | 0.209592 | 6.3:+60394625 | None:intergenic |
| AAGAGGAGAAAATAATAAAA+AGG | 0.299777 | 6.3:-60394303 | MS.gene33235:CDS |
| GAAAAGAACACAAAGATAAA+AGG | 0.316710 | 6.3:+60394689 | None:intergenic |
| GGAATTGAATCATCCAGATA+TGG | 0.324652 | 6.3:-60394638 | MS.gene33235:CDS |
| AGGAGGAGGTGGACAAAATC+AGG | 0.330426 | 6.3:-60394363 | MS.gene33235:CDS |
| GGATCAAGCTCAGGAGAAAA+TGG | 0.374472 | 6.3:-60394404 | MS.gene33235:CDS |
| GCTGGCCGGGAGGATTAAAA+AGG | 0.387508 | 6.3:-60394466 | MS.gene33235:CDS |
| AATGGACCGCTTGGCAAAGC+TGG | 0.395837 | 6.3:-60394484 | MS.gene33235:CDS |
| ATTGGTAAAGAAGGCCAAAA+AGG | 0.400206 | 6.3:-60394443 | MS.gene33235:CDS |
| GACCGCTTGGCAAAGCTGGC+CGG | 0.427719 | 6.3:-60394480 | MS.gene33235:CDS |
| ACCGCTTGGCAAAGCTGGCC+GGG | 0.446723 | 6.3:-60394479 | MS.gene33235:CDS |
| TTGGGATACAGAATTGGAAA+TGG | 0.458344 | 6.3:-60394502 | MS.gene33235:CDS |
| GAAGGAGTTGCGCAAGGAGG+AGG | 0.483849 | 6.3:-60394377 | MS.gene33235:CDS |
| CTAATTCTTGTCAATGTTGT+GGG | 0.486977 | 6.3:-60394659 | MS.gene33235:CDS |
| GAACACAAAGATAAAAGGCT+TGG | 0.496731 | 6.3:+60394694 | None:intergenic |
| AGAATTGGAAATGGACCGCT+TGG | 0.497786 | 6.3:-60394493 | MS.gene33235:CDS |
| GGAGTTGCGCAAGGAGGAGG+TGG | 0.504922 | 6.3:-60394374 | MS.gene33235:CDS |
| GGCTCAGGTGGATCAAGCTC+AGG | 0.505461 | 6.3:-60394413 | MS.gene33235:CDS |
| GAGCTTGATCCACCTGAGCC+CGG | 0.516745 | 6.3:+60394416 | None:intergenic |
| AAAGGTGAACCGGGCTCAGG+TGG | 0.518166 | 6.3:-60394425 | MS.gene33235:CDS |
| TCAGGAGAAAATGGCGGTGA+AGG | 0.543194 | 6.3:-60394395 | MS.gene33235:CDS |
| ACTAATATAACATAAAATCA+TGG | 0.545693 | 6.3:+60394264 | None:intergenic |
| GAATTGAATCATCCAGATAT+GGG | 0.550487 | 6.3:-60394637 | MS.gene33235:CDS |
| GACAAAGAAAGTGCGACTGA+TGG | 0.553843 | 6.3:-60394332 | MS.gene33235:CDS |
| GCGACTGATGGAGGAAGAAG+AGG | 0.555550 | 6.3:-60394320 | MS.gene33235:CDS |
| GCTAATTCTTGTCAATGTTG+TGG | 0.561586 | 6.3:-60394660 | MS.gene33235:CDS |
| TTGATTTGTAGTAGATTCAA+CGG | 0.575724 | 6.3:-60394543 | MS.gene33235:intron |
| AAAGAAAGTGCGACTGATGG+AGG | 0.575733 | 6.3:-60394329 | MS.gene33235:CDS |
| GGAGGAGGTGGACAAAATCA+GGG | 0.611095 | 6.3:-60394362 | MS.gene33235:CDS |
| GGCGGTGAAGGAGTTGCGCA+AGG | 0.611945 | 6.3:-60394383 | MS.gene33235:CDS |
| TCCCGGCCAGCTTTGCCAAG+CGG | 0.616564 | 6.3:+60394478 | None:intergenic |
| GCTTGGCAAAGCTGGCCGGG+AGG | 0.664993 | 6.3:-60394476 | MS.gene33235:CDS |
| GGTGAAGGAGTTGCGCAAGG+AGG | 0.712010 | 6.3:-60394380 | MS.gene33235:CDS |
| TCAAGCTCAGGAGAAAATGG+CGG | 0.716902 | 6.3:-60394401 | MS.gene33235:CDS |
| GGAGGTGGACAAAATCAGGG+TGG | 0.749319 | 6.3:-60394359 | MS.gene33235:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGAAATCAATACAAAAATT+GGG | - | chr6.3:60394446-60394465 | MS.gene33235:CDS | 15.0% |
| !! | GAAAATAATAAAAAGGATTA+TGG | - | chr6.3:60394670-60394689 | MS.gene33235:CDS | 15.0% |
| !! | AAGAGGAGAAAATAATAAAA+AGG | - | chr6.3:60394663-60394682 | MS.gene33235:CDS | 20.0% |
| !! | AATAATAAAAAGGATTATGG+AGG | - | chr6.3:60394673-60394692 | MS.gene33235:CDS | 20.0% |
| !! | GAAGAAATCAATACAAAAAT+TGG | - | chr6.3:60394445-60394464 | MS.gene33235:CDS | 20.0% |
| !! | TTAGATATAACAAATGAAGA+AGG | + | chr6.3:60394390-60394409 | None:intergenic | 20.0% |
| ! | GAAAAGAACACAAAGATAAA+AGG | + | chr6.3:60394280-60394299 | None:intergenic | 25.0% |
| !! | TTAAAAAGGATTGGTAAAGA+AGG | - | chr6.3:60394514-60394533 | MS.gene33235:CDS | 25.0% |
| !!! | TTGATTTGTAGTAGATTCAA+CGG | - | chr6.3:60394423-60394442 | MS.gene33235:CDS | 25.0% |
| ATAAAAAGGATTATGGAGGT+TGG | - | chr6.3:60394677-60394696 | MS.gene33235:CDS | 30.0% | |
| CAAAAATTGGGATACAGAAT+TGG | - | chr6.3:60394458-60394477 | MS.gene33235:CDS | 30.0% | |
| CTAATTCTTGTCAATGTTGT+GGG | - | chr6.3:60394307-60394326 | MS.gene33235:CDS | 30.0% | |
| GAATTGAATCATCCAGATAT+GGG | - | chr6.3:60394329-60394348 | MS.gene33235:CDS | 30.0% | |
| TAAAAAGGATTATGGAGGTT+GGG | - | chr6.3:60394678-60394697 | MS.gene33235:CDS | 30.0% | |
| ATTGGTAAAGAAGGCCAAAA+AGG | - | chr6.3:60394523-60394542 | MS.gene33235:CDS | 35.0% | |
| CATACATTGTTTCCCATATC+TGG | + | chr6.3:60394344-60394363 | None:intergenic | 35.0% | |
| GAACACAAAGATAAAAGGCT+TGG | + | chr6.3:60394275-60394294 | None:intergenic | 35.0% | |
| GCTAATTCTTGTCAATGTTG+TGG | - | chr6.3:60394306-60394325 | MS.gene33235:CDS | 35.0% | |
| GGAATTGAATCATCCAGATA+TGG | - | chr6.3:60394328-60394347 | MS.gene33235:CDS | 35.0% | |
| TTGGGATACAGAATTGGAAA+TGG | - | chr6.3:60394464-60394483 | MS.gene33235:CDS | 35.0% | |
| ! | CCAATCCTTTTTAATCCTCC+CGG | + | chr6.3:60394508-60394527 | None:intergenic | 40.0% |
| AGAAGGCCAAAAAGGTGAAC+CGG | - | chr6.3:60394531-60394550 | MS.gene33235:CDS | 45.0% | |
| AGAATTGGAAATGGACCGCT+TGG | - | chr6.3:60394473-60394492 | MS.gene33235:CDS | 45.0% | |
| CCGGGAGGATTAAAAAGGAT+TGG | - | chr6.3:60394505-60394524 | MS.gene33235:CDS | 45.0% | |
| GGATCAAGCTCAGGAGAAAA+TGG | - | chr6.3:60394562-60394581 | MS.gene33235:intron | 45.0% | |
| TCAAGCTCAGGAGAAAATGG+CGG | - | chr6.3:60394565-60394584 | MS.gene33235:intron | 45.0% | |
| ! | AAAGAAAGTGCGACTGATGG+AGG | - | chr6.3:60394637-60394656 | MS.gene33235:CDS | 45.0% |
| ! | GACAAAGAAAGTGCGACTGA+TGG | - | chr6.3:60394634-60394653 | MS.gene33235:CDS | 45.0% |
| AGGAGGAGGTGGACAAAATC+AGG | - | chr6.3:60394603-60394622 | MS.gene33235:intron | 50.0% | |
| GAAGGCCAAAAAGGTGAACC+GGG | - | chr6.3:60394532-60394551 | MS.gene33235:CDS | 50.0% | |
| GGAGGAGGTGGACAAAATCA+GGG | - | chr6.3:60394604-60394623 | MS.gene33235:intron | 50.0% | |
| TCAGGAGAAAATGGCGGTGA+AGG | - | chr6.3:60394571-60394590 | MS.gene33235:intron | 50.0% | |
| AATGGACCGCTTGGCAAAGC+TGG | - | chr6.3:60394482-60394501 | MS.gene33235:CDS | 55.0% | |
| CAAAAAGGTGAACCGGGCTC+AGG | - | chr6.3:60394538-60394557 | MS.gene33235:intron | 55.0% | |
| GCGACTGATGGAGGAAGAAG+AGG | - | chr6.3:60394646-60394665 | MS.gene33235:CDS | 55.0% | |
| GCTGGCCGGGAGGATTAAAA+AGG | - | chr6.3:60394500-60394519 | MS.gene33235:CDS | 55.0% | |
| GGAGGTGGACAAAATCAGGG+TGG | - | chr6.3:60394607-60394626 | MS.gene33235:intron | 55.0% | |
| ! | CTGAGCCCGGTTCACCTTTT+TGG | + | chr6.3:60394540-60394559 | None:intergenic | 55.0% |
| AAAGGTGAACCGGGCTCAGG+TGG | - | chr6.3:60394541-60394560 | MS.gene33235:intron | 60.0% | |
| GAAGGAGTTGCGCAAGGAGG+AGG | - | chr6.3:60394589-60394608 | MS.gene33235:intron | 60.0% | |
| GAGCTTGATCCACCTGAGCC+CGG | + | chr6.3:60394553-60394572 | None:intergenic | 60.0% | |
| GGCTCAGGTGGATCAAGCTC+AGG | - | chr6.3:60394553-60394572 | MS.gene33235:intron | 60.0% | |
| GGTGAAGGAGTTGCGCAAGG+AGG | - | chr6.3:60394586-60394605 | MS.gene33235:intron | 60.0% | |
| ACCGCTTGGCAAAGCTGGCC+GGG | - | chr6.3:60394487-60394506 | MS.gene33235:CDS | 65.0% | |
| GACCGCTTGGCAAAGCTGGC+CGG | - | chr6.3:60394486-60394505 | MS.gene33235:CDS | 65.0% | |
| GGAGTTGCGCAAGGAGGAGG+TGG | - | chr6.3:60394592-60394611 | MS.gene33235:intron | 65.0% | |
| GGCGGTGAAGGAGTTGCGCA+AGG | - | chr6.3:60394583-60394602 | MS.gene33235:intron | 65.0% | |
| TCCCGGCCAGCTTTGCCAAG+CGG | + | chr6.3:60394491-60394510 | None:intergenic | 65.0% | |
| GCTTGGCAAAGCTGGCCGGG+AGG | - | chr6.3:60394490-60394509 | MS.gene33235:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 60394265 | 60394723 | 60394265 | ID=MS.gene33235 |
| chr6.3 | mRNA | 60394265 | 60394723 | 60394265 | ID=MS.gene33235.t1;Parent=MS.gene33235 |
| chr6.3 | exon | 60394630 | 60394723 | 60394630 | ID=MS.gene33235.t1.exon1;Parent=MS.gene33235.t1 |
| chr6.3 | CDS | 60394630 | 60394723 | 60394630 | ID=cds.MS.gene33235.t1;Parent=MS.gene33235.t1 |
| chr6.3 | exon | 60394265 | 60394554 | 60394265 | ID=MS.gene33235.t1.exon2;Parent=MS.gene33235.t1 |
| chr6.3 | CDS | 60394265 | 60394554 | 60394265 | ID=cds.MS.gene33235.t1;Parent=MS.gene33235.t1 |
| Gene Sequence |
| Protein sequence |
