AlfalfaGEDB | MS.gene057406

M. sativa cultivar XinJiangDaYe / MS.gene057406

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057406.t1	XP_003595324.1	74.1	135	34	1	1	134	1	135	2.50E-27	131.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057406.t1	A7KHI0	74.1	135	34	1	1	134	1	135	1.8e-27	131.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene057406	MS.gene33235	0.814553	1.45E-51	-1.69E-46
MS.gene057406	MS.gene46123	0.814553	1.45E-51	-1.69E-46
MS.gene057406	MS.gene57866	0.814553	1.45E-51	-1.69E-46
MS.gene057406	MS.gene81175	0.814553	1.45E-51	-1.69E-46
MS.gene057406	MS.gene91566	0.814553	1.45E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057406.t1	MTR_2g042520	83.704	135	21	1	1	134	1	135	3.71e-47	149
MS.gene057406.t1	MTR_2g042510	85.470	117	16	1	1	116	1	117	3.75e-40	130
MS.gene057406.t1	MTR_2g042480	67.692	65	21	0	1	65	1	65	6.78e-15	66.2
MS.gene057406.t1	MTR_2g042470	49.438	89	40	2	1	86	1	87	5.30e-13	61.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 42 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATACATTGTTTCTCATCTT+TGG	0.185462	2.1:-50991672	None:intergenic
GTTGGGGATATTTGAAGAAT+TGG	0.269904	2.1:+50992033	MS.gene057406:CDS
GGAGGAGAAAATAATAAAAT+AGG	0.275351	2.1:+50992011	MS.gene057406:CDS
AATTCAAATTAAAAGTTTCC+TGG	0.326557	2.1:+50991814	MS.gene057406:CDS
AGAAAATAATAAAATAGGTT+GGG	0.370670	2.1:+50992016	MS.gene057406:CDS
TTGGGGATATTTGAAGAATT+GGG	0.374101	2.1:+50992034	MS.gene057406:CDS
GGTCCAAGCTCCACGTTTCC+AGG	0.411624	2.1:-50991832	None:intergenic
CATTGTTTCTCATCTTTGGA+TGG	0.412855	2.1:-50991668	None:intergenic
GGATCAGGCTCAGGAGGAAA+TGG	0.424839	2.1:+50991906	MS.gene057406:CDS
GGAGGATCAAAAGGTGGATC+AGG	0.449291	2.1:+50991891	MS.gene057406:CDS
GAGAAAATAATAAAATAGGT+TGG	0.454702	2.1:+50992015	MS.gene057406:CDS
GGAAACGTGGAGCTTGGACC+TGG	0.465325	2.1:+50991835	MS.gene057406:CDS
TTTCCTGGAAACGTGGAGCT+TGG	0.471240	2.1:+50991829	MS.gene057406:CDS
GAAGGAGGTGCACAAGGTGG+AGG	0.476976	2.1:+50991933	MS.gene057406:CDS
TCAGGAGGAAATGGCGGTGA+AGG	0.484259	2.1:+50991915	MS.gene057406:CDS
GGTGGTAAAGGAGGATCAAA+AGG	0.489233	2.1:+50991882	MS.gene057406:CDS
GGACTTGATGCAAGAGGAGG+AGG	0.494557	2.1:+50991990	MS.gene057406:CDS
AGCAAAAGTGAAGGGAATCA+AGG	0.501265	2.1:+50992080	MS.gene057406:CDS
GGAGGTGCACAAGGTGGAGG+TGG	0.511180	2.1:+50991936	MS.gene057406:CDS
GAAAATAATAAAATAGGTTG+GGG	0.511887	2.1:+50992017	MS.gene057406:CDS
GTAAAACGAAAGGGTGGTAA+AGG	0.517947	2.1:+50991870	MS.gene057406:CDS
CTTGATGCAAGAGGAGGAGG+AGG	0.521929	2.1:+50991993	MS.gene057406:CDS
TCAAAAGGTGGATCAGGCTC+AGG	0.526089	2.1:+50991897	MS.gene057406:CDS
GACATTAGTAGCAAAAGTGA+AGG	0.540827	2.1:+50992071	MS.gene057406:CDS
GTCTATGCAGTAAAACGAAA+GGG	0.542045	2.1:+50991861	MS.gene057406:CDS
AAGAATTGGGAAGGTTACAA+TGG	0.546843	2.1:+50992047	MS.gene057406:CDS
GGCGGTGAAGGAGGTGCACA+AGG	0.574852	2.1:+50991927	MS.gene057406:CDS
AGTCTATGCAGTAAAACGAA+AGG	0.584016	2.1:+50991860	MS.gene057406:CDS
AAAGGTGGATCAGGCTCAGG+AGG	0.587959	2.1:+50991900	MS.gene057406:CDS
GGAGGAAATGGCGGTGAAGG+AGG	0.588195	2.1:+50991918	MS.gene057406:CDS
GCAAAAGTGAAGGGAATCAA+GGG	0.589925	2.1:+50992081	MS.gene057406:CDS
GGAAATGGACTTGATGCAAG+AGG	0.591685	2.1:+50991984	MS.gene057406:CDS
AAACGAAAGGGTGGTAAAGG+AGG	0.609087	2.1:+50991873	MS.gene057406:CDS
TTAAAAGTTTCCTGGAAACG+TGG	0.609903	2.1:+50991822	MS.gene057406:CDS
GGATATTTGAAGAATTGGGA+AGG	0.610393	2.1:+50992038	MS.gene057406:CDS
CATTCATCTGGATTTCATGG+AGG	0.620659	2.1:-50992104	None:intergenic
GGTGAAGGAGGTGCACAAGG+TGG	0.651404	2.1:+50991930	MS.gene057406:CDS
ACATTAGTAGCAAAAGTGAA+GGG	0.652694	2.1:+50992072	MS.gene057406:CDS
AATGGACTTGATGCAAGAGG+AGG	0.654638	2.1:+50991987	MS.gene057406:CDS
TATGCAGTAAAACGAAAGGG+TGG	0.676481	2.1:+50991864	MS.gene057406:CDS
TCAGGCTCAGGAGGAAATGG+CGG	0.681849	2.1:+50991909	MS.gene057406:CDS
GGTAAAGGAGGATCAAAAGG+TGG	0.696385	2.1:+50991885	MS.gene057406:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AGAAAATAATAAAATAGGTT+GGG	+	chr2.1:50992016-50992035	MS.gene057406:CDS	15.0%
!!	GTATTGAATATAATAAAAGA+AGG	-	chr2.1:50991728-50991747	None:intergenic	15.0%
!!	AATTCAAATTAAAAGTTTCC+TGG	+	chr2.1:50991814-50991833	MS.gene057406:CDS	20.0%
!!	GAAAATAATAAAATAGGTTG+GGG	+	chr2.1:50992017-50992036	MS.gene057406:CDS	20.0%
!!	GAGAAAATAATAAAATAGGT+TGG	+	chr2.1:50992015-50992034	MS.gene057406:CDS	20.0%
!	GGAGGAGAAAATAATAAAAT+AGG	+	chr2.1:50992011-50992030	MS.gene057406:CDS	25.0%
!!	TATACATTGTTTCTCATCTT+TGG	-	chr2.1:50991675-50991694	None:intergenic	25.0%
	ACATTAGTAGCAAAAGTGAA+GGG	+	chr2.1:50992072-50992091	MS.gene057406:CDS	30.0%
	ATCGAAGATGAAAAAGATGA+AGG	+	chr2.1:50991963-50991982	MS.gene057406:CDS	30.0%
	GATGAAAAAGATGAAGGAAA+TGG	+	chr2.1:50991969-50991988	MS.gene057406:CDS	30.0%
	TTGGGGATATTTGAAGAATT+GGG	+	chr2.1:50992034-50992053	MS.gene057406:CDS	30.0%
!!	AGTGTTTGTTAATTTGCAGT+TGG	+	chr2.1:50991780-50991799	MS.gene057406:intron	30.0%
!!!	ACTAGTTTTTATCTCTGTTG+TGG	+	chr2.1:50991637-50991656	MS.gene057406:CDS	30.0%
	AAGAATTGGGAAGGTTACAA+TGG	+	chr2.1:50992047-50992066	MS.gene057406:CDS	35.0%
	AGTCTATGCAGTAAAACGAA+AGG	+	chr2.1:50991860-50991879	MS.gene057406:CDS	35.0%
	GACATTAGTAGCAAAAGTGA+AGG	+	chr2.1:50992071-50992090	MS.gene057406:CDS	35.0%
	GGATATTTGAAGAATTGGGA+AGG	+	chr2.1:50992038-50992057	MS.gene057406:CDS	35.0%
	GTCTATGCAGTAAAACGAAA+GGG	+	chr2.1:50991861-50991880	MS.gene057406:CDS	35.0%
	GTTGGGGATATTTGAAGAAT+TGG	+	chr2.1:50992033-50992052	MS.gene057406:CDS	35.0%
	TTAAAAGTTTCCTGGAAACG+TGG	+	chr2.1:50991822-50991841	MS.gene057406:CDS	35.0%
!	CATTGTTTCTCATCTTTGGA+TGG	-	chr2.1:50991671-50991690	None:intergenic	35.0%
!	TTTCGTTTTACTGCATAGAC+TGG	-	chr2.1:50991861-50991880	None:intergenic	35.0%
	AGCAAAAGTGAAGGGAATCA+AGG	+	chr2.1:50992080-50992099	MS.gene057406:CDS	40.0%
	GCAAAAGTGAAGGGAATCAA+GGG	+	chr2.1:50992081-50992100	MS.gene057406:CDS	40.0%
	GTAAAACGAAAGGGTGGTAA+AGG	+	chr2.1:50991870-50991889	MS.gene057406:CDS	40.0%
	TATGCAGTAAAACGAAAGGG+TGG	+	chr2.1:50991864-50991883	MS.gene057406:CDS	40.0%
	AAACGAAAGGGTGGTAAAGG+AGG	+	chr2.1:50991873-50991892	MS.gene057406:CDS	45.0%
	GGTAAAGGAGGATCAAAAGG+TGG	+	chr2.1:50991885-50991904	MS.gene057406:CDS	45.0%
	GGTGGTAAAGGAGGATCAAA+AGG	+	chr2.1:50991882-50991901	MS.gene057406:CDS	45.0%
!	AATGGACTTGATGCAAGAGG+AGG	+	chr2.1:50991987-50992006	MS.gene057406:CDS	45.0%
!	GGAAATGGACTTGATGCAAG+AGG	+	chr2.1:50991984-50992003	MS.gene057406:CDS	45.0%
!!	TTTTACTGCATAGACTGGCC+AGG	-	chr2.1:50991856-50991875	None:intergenic	45.0%
	GGAGGATCAAAAGGTGGATC+AGG	+	chr2.1:50991891-50991910	MS.gene057406:CDS	50.0%
	TCAAAAGGTGGATCAGGCTC+AGG	+	chr2.1:50991897-50991916	MS.gene057406:CDS	50.0%
	TTTCCTGGAAACGTGGAGCT+TGG	+	chr2.1:50991829-50991848	MS.gene057406:CDS	50.0%
	AAAGGTGGATCAGGCTCAGG+AGG	+	chr2.1:50991900-50991919	MS.gene057406:CDS	55.0%
	CTTGATGCAAGAGGAGGAGG+AGG	+	chr2.1:50991993-50992012	MS.gene057406:CDS	55.0%
	GGACTTGATGCAAGAGGAGG+AGG	+	chr2.1:50991990-50992009	MS.gene057406:CDS	55.0%
	GGATCAGGCTCAGGAGGAAA+TGG	+	chr2.1:50991906-50991925	MS.gene057406:CDS	55.0%
	TCAGGAGGAAATGGCGGTGA+AGG	+	chr2.1:50991915-50991934	MS.gene057406:CDS	55.0%
	TCAGGCTCAGGAGGAAATGG+CGG	+	chr2.1:50991909-50991928	MS.gene057406:CDS	55.0%
	GGAAACGTGGAGCTTGGACC+TGG	+	chr2.1:50991835-50991854	MS.gene057406:CDS	60.0%
	GGAGGAAATGGCGGTGAAGG+AGG	+	chr2.1:50991918-50991937	MS.gene057406:CDS	60.0%
	GGTCCAAGCTCCACGTTTCC+AGG	-	chr2.1:50991835-50991854	None:intergenic	60.0%
!	GAAGGAGGTGCACAAGGTGG+AGG	+	chr2.1:50991933-50991952	MS.gene057406:CDS	60.0%
!	GGTGAAGGAGGTGCACAAGG+TGG	+	chr2.1:50991930-50991949	MS.gene057406:CDS	60.0%
!	GGAGGTGCACAAGGTGGAGG+TGG	+	chr2.1:50991936-50991955	MS.gene057406:CDS	65.0%
!	GGCGGTGAAGGAGGTGCACA+AGG	+	chr2.1:50991927-50991946	MS.gene057406:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	50991596	50992112	50991596	ID=MS.gene057406
chr2.1	mRNA	50991596	50992112	50991596	ID=MS.gene057406.t1;Parent=MS.gene057406
chr2.1	exon	50991596	50991689	50991596	ID=MS.gene057406.t1.exon1;Parent=MS.gene057406.t1
chr2.1	CDS	50991596	50991689	50991596	ID=cds.MS.gene057406.t1;Parent=MS.gene057406.t1
chr2.1	exon	50991799	50992112	50991799	ID=MS.gene057406.t1.exon2;Parent=MS.gene057406.t1
chr2.1	CDS	50991799	50992112	50991799	ID=cds.MS.gene057406.t1;Parent=MS.gene057406.t1

Gene Sequence

>MS.gene057406

ATGAAAACAAAGTCTATTATCTCATTCTTCTTTATTTGTACACTAGTTTTTATCTCTGTTGTGGCAACTGAGCCATCCAAAGATGAGAAACAATGTATATAAGCATAAATATACACACATATTTTTTTTCCTTCTTTTATTATATTCAATACAAAGATTTTTTTTTTGAAGAAATTTAATACAAAGTGTTTGTTAATTTGCAGTTGGTGAAATAGAAGAATTCAAATTAAAAGTTTCCTGGAAACGTGGAGCTTGGACCTGGCCAGTCTATGCAGTAAAACGAAAGGGTGGTAAAGGAGGATCAAAAGGTGGATCAGGCTCAGGAGGAAATGGCGGTGAAGGAGGTGCACAAGGTGGAGGTGGACAAATCGAAGATGAAAAAGATGAAGGAAATGGACTTGATGCAAGAGGAGGAGGAGGAGAAAATAATAAAATAGGTTGGGGATATTTGAAGAATTGGGAAGGTTACAATGGTGACATTAGTAGCAAAAGTGAAGGGAATCAAGGGCCTCCATGA

Protein sequence

>MS.gene057406.t1

MKTKSIISFFFICTLVFISVVATEPSKDEKQFGEIEEFKLKVSWKRGAWTWPVYAVKRKGGKGGSKGGSGSGGNGGEGGAQGGGGQIEDEKDEGNGLDARGGGGENNKIGWGYLKNWEGYNGDISSKSEGNQGPP