Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33292.t1 | XP_024639030.1 | 95.1 | 122 | 6 | 0 | 1 | 122 | 161 | 282 | 7.90E-66 | 259.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33292.t1 | G7K8A0 | 95.1 | 122 | 6 | 0 | 1 | 122 | 161 | 282 | 5.7e-66 | 259.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052308 | MS.gene33292 | 0.808956 | 2.40E-50 | -1.69E-46 |
| MS.gene056041 | MS.gene33292 | 0.802078 | 6.65E-49 | -1.69E-46 |
| MS.gene058853 | MS.gene33292 | 0.806115 | 9.62E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCACATCATCTTACAATAA+TGG | 0.287235 | 5.2:-27474105 | MS.gene33292:CDS |
| CTTCAATGCAGTTCATATAC+AGG | 0.342561 | 5.2:-27474333 | MS.gene33292:CDS |
| GATCCTCAAAAGTGGAACTA+TGG | 0.374420 | 5.2:-27474282 | MS.gene33292:CDS |
| AGGTTAATATTAGTACTAAT+AGG | 0.427315 | 5.2:+27474426 | None:intergenic |
| GTTTATACACCGGTTAATCT+TGG | 0.430829 | 5.2:-27474384 | MS.gene33292:CDS |
| TAAACACAACATGTCGAAAT+CGG | 0.436820 | 5.2:+27474402 | None:intergenic |
| GTAGTAGTGTTAATTCCATT+AGG | 0.443608 | 5.2:+27474138 | None:intergenic |
| GCTGCATGTGAAAGGAGCTT+TGG | 0.444173 | 5.2:-27474207 | MS.gene33292:CDS |
| CTCATCTTATAAGCAATTGA+TGG | 0.460174 | 5.2:-27473723 | MS.gene33292:CDS |
| AACAAATTGATCCTCAAAAG+TGG | 0.460285 | 5.2:-27474290 | MS.gene33292:CDS |
| AAAGCTGGACCAAGATTAAC+CGG | 0.476906 | 5.2:+27474375 | None:intergenic |
| GCAATTGATGGTAACAATCA+AGG | 0.481266 | 5.2:-27473711 | MS.gene33292:CDS |
| TGAAGATCCATATCAAAAGC+TGG | 0.499024 | 5.2:+27474360 | None:intergenic |
| TCACATGCAGCACAAGAGCT+AGG | 0.560587 | 5.2:+27474219 | None:intergenic |
| GACATGTTGTGTTTATACAC+CGG | 0.561125 | 5.2:-27474394 | MS.gene33292:CDS |
| GCTCTTGTGCTGCATGTGAA+AGG | 0.562347 | 5.2:-27474215 | MS.gene33292:CDS |
| CACAACATGTCGAAATCGGA+AGG | 0.597606 | 5.2:+27474406 | None:intergenic |
| AATCAAGGTTATGATCACCT+AGG | 0.696642 | 5.2:-27473696 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAAATGAATGAAAAAATT+AGG | + | chr5.2:27474308-27474327 | None:intergenic | 10.0% |
| !! | AGGTTAATATTAGTACTAAT+AGG | + | chr5.2:27473701-27473720 | None:intergenic | 20.0% |
| !! | ATGTAATTGACAAATAAGAA+AGG | + | chr5.2:27474072-27474091 | None:intergenic | 20.0% |
| !! | TGTAATTGACAAATAAGAAA+GGG | + | chr5.2:27474071-27474090 | None:intergenic | 20.0% |
| !!! | ACACTAAAATTTAAGATTAG+AGG | - | chr5.2:27474142-27474161 | MS.gene33292:CDS | 20.0% |
| !!! | CACTAAAATTTAAGATTAGA+GGG | - | chr5.2:27474143-27474162 | MS.gene33292:CDS | 20.0% |
| !!! | TACAATAATGGTTTTAGAAA+TGG | - | chr5.2:27474031-27474050 | MS.gene33292:intron | 20.0% |
| !!! | TTTAAAACTATTATCAGTGT+CGG | - | chr5.2:27474237-27474256 | MS.gene33292:CDS | 20.0% |
| ! | TTAAATGTAACAAGTTGTGT+TGG | + | chr5.2:27474205-27474224 | None:intergenic | 25.0% |
| ! | TTCACATCATCTTACAATAA+TGG | - | chr5.2:27474019-27474038 | MS.gene33292:intron | 25.0% |
| !!! | TCATTTTTAATGACTTGTTC+TGG | - | chr5.2:27474319-27474338 | MS.gene33292:CDS | 25.0% |
| AACAAATTGATCCTCAAAAG+TGG | - | chr5.2:27473834-27473853 | MS.gene33292:intron | 30.0% | |
| CTCATCTTATAAGCAATTGA+TGG | - | chr5.2:27474401-27474420 | MS.gene33292:CDS | 30.0% | |
| GTAGTAGTGTTAATTCCATT+AGG | + | chr5.2:27473989-27474008 | None:intergenic | 30.0% | |
| TAAACACAACATGTCGAAAT+CGG | + | chr5.2:27473725-27473744 | None:intergenic | 30.0% | |
| ! | TTTTTATGCAACTGTCCTAA+TGG | - | chr5.2:27473971-27473990 | MS.gene33292:intron | 30.0% |
| AATCAAGGTTATGATCACCT+AGG | - | chr5.2:27474428-27474447 | MS.gene33292:CDS | 35.0% | |
| CTTCAATGCAGTTCATATAC+AGG | - | chr5.2:27473791-27473810 | MS.gene33292:intron | 35.0% | |
| GACATGTTGTGTTTATACAC+CGG | - | chr5.2:27473730-27473749 | MS.gene33292:intron | 35.0% | |
| TGAAGATCCATATCAAAAGC+TGG | + | chr5.2:27473767-27473786 | None:intergenic | 35.0% | |
| ! | CTTTTGCATGGTCTTTGATT+AGG | - | chr5.2:27474378-27474397 | MS.gene33292:CDS | 35.0% |
| ! | GCAATTGATGGTAACAATCA+AGG | - | chr5.2:27474413-27474432 | MS.gene33292:CDS | 35.0% |
| ! | GTTTATACACCGGTTAATCT+TGG | - | chr5.2:27473740-27473759 | MS.gene33292:intron | 35.0% |
| !! | ATTCCATAGTTCCACTTTTG+AGG | + | chr5.2:27473848-27473867 | None:intergenic | 35.0% |
| !! | GGTTGATATATGCTTTTGCA+TGG | - | chr5.2:27474366-27474385 | MS.gene33292:CDS | 35.0% |
| AAAGCTGGACCAAGATTAAC+CGG | + | chr5.2:27473752-27473771 | None:intergenic | 40.0% | |
| AGCAAACCCTGAAAACAGAA+AGG | + | chr5.2:27474345-27474364 | None:intergenic | 40.0% | |
| GATCCTCAAAAGTGGAACTA+TGG | - | chr5.2:27473842-27473861 | MS.gene33292:intron | 40.0% | |
| ! | TCTTGGTCCAGCTTTTGATA+TGG | - | chr5.2:27473757-27473776 | MS.gene33292:intron | 40.0% |
| !! | ATCAGTGTCGGTGTTATGTT+GGG | - | chr5.2:27474249-27474268 | MS.gene33292:CDS | 40.0% |
| !! | TATCAGTGTCGGTGTTATGT+TGG | - | chr5.2:27474248-27474267 | MS.gene33292:CDS | 40.0% |
| !! | TTCTGGCCTTTCTGTTTTCA+GGG | - | chr5.2:27474336-27474355 | MS.gene33292:CDS | 40.0% |
| !! | TTCTGTTTTCAGGGTTTGCT+TGG | - | chr5.2:27474345-27474364 | MS.gene33292:CDS | 40.0% |
| !!! | GAAAGGAGCTTTGGATTTTG+TGG | - | chr5.2:27473926-27473945 | MS.gene33292:intron | 40.0% |
| CACAACATGTCGAAATCGGA+AGG | + | chr5.2:27473721-27473740 | None:intergenic | 45.0% | |
| !! | GTTCTGGCCTTTCTGTTTTC+AGG | - | chr5.2:27474335-27474354 | MS.gene33292:CDS | 45.0% |
| !!! | TTTTTTTTTTTTTTTTGAAA+AGG | - | chr5.2:27474118-27474137 | MS.gene33292:CDS | 5.0% |
| GCTCTTGTGCTGCATGTGAA+AGG | - | chr5.2:27473909-27473928 | MS.gene33292:intron | 50.0% | |
| GCTGCATGTGAAAGGAGCTT+TGG | - | chr5.2:27473917-27473936 | MS.gene33292:intron | 50.0% | |
| TCACATGCAGCACAAGAGCT+AGG | + | chr5.2:27473908-27473927 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 27473697 | 27474449 | 27473697 | ID=MS.gene33292 |
| chr5.2 | mRNA | 27473697 | 27474449 | 27473697 | ID=MS.gene33292.t1;Parent=MS.gene33292 |
| chr5.2 | exon | 27474094 | 27474449 | 27474094 | ID=MS.gene33292.t1.exon1;Parent=MS.gene33292.t1 |
| chr5.2 | CDS | 27474094 | 27474449 | 27474094 | ID=cds.MS.gene33292.t1;Parent=MS.gene33292.t1 |
| chr5.2 | exon | 27473697 | 27473746 | 27473697 | ID=MS.gene33292.t1.exon2;Parent=MS.gene33292.t1 |
| chr5.2 | CDS | 27473697 | 27473746 | 27473697 | ID=cds.MS.gene33292.t1;Parent=MS.gene33292.t1 |
| Gene Sequence |
| Protein sequence |
