AlfalfaGEDB | MS.gene33425

M. sativa cultivar XinJiangDaYe / MS.gene33425

KEGG: -
GO: -
PFAM: PF03018.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33425.t1	XP_013454798.1	96.3	108	4	0	1	108	89	196	4.80E-53	216.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33425.t1	Q9C523	73.1	108	29	0	1	108	78	185	1.4e-40	166.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33425.t1	A0A072UGG3	96.3	108	4	0	1	108	89	196	3.4e-53	216.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049887	MS.gene33425	0.816378	5.70E-52	-1.69E-46
MS.gene050807	MS.gene33425	0.817555	3.10E-52	-1.69E-46
MS.gene051719	MS.gene33425	0.812403	4.31E-51	-1.69E-46
MS.gene052133	MS.gene33425	0.818182	2.24E-52	-1.69E-46
MS.gene052665	MS.gene33425	0.827394	1.60E-54	-1.69E-46
MS.gene052732	MS.gene33425	0.812999	3.20E-51	-1.69E-46
MS.gene053144	MS.gene33425	0.824096	9.71E-54	-1.69E-46
MS.gene053581	MS.gene33425	0.829991	3.78E-55	-1.69E-46
MS.gene054584	MS.gene33425	0.820244	7.60E-53	-1.69E-46
MS.gene055891	MS.gene33425	0.802973	4.35E-49	-1.69E-46
MS.gene057057	MS.gene33425	0.803039	4.22E-49	-1.69E-46
MS.gene059171	MS.gene33425	0.823371	1.44E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33425.t1	MTR_4g013345	96.296	108	4	0	1	108	89	196	7.86e-76	222
MS.gene33425.t1	MTR_4g013330	89.815	108	11	0	1	108	92	199	1.48e-70	209
MS.gene33425.t1	MTR_4g013350	89.815	108	11	0	1	108	90	197	3.32e-69	206
MS.gene33425.t1	MTR_4g013320	79.630	108	22	0	1	108	92	199	7.84e-63	189
MS.gene33425.t1	MTR_4g013325	75.926	108	26	0	1	108	92	199	3.56e-59	180
MS.gene33425.t1	MTR_7g070390	74.074	108	28	0	1	108	85	192	7.92e-59	179
MS.gene33425.t1	MTR_4g013335	75.000	108	27	0	1	108	89	196	1.83e-58	178
MS.gene33425.t1	MTR_4g013310	71.296	108	29	1	1	108	92	197	5.14e-52	162
MS.gene33425.t1	MTR_1g054525	71.296	108	29	1	1	108	87	192	2.99e-51	160
MS.gene33425.t1	MTR_4g013385	69.444	108	33	0	1	108	83	190	4.74e-51	159
MS.gene33425.t1	MTR_0433s0040	69.444	108	33	0	1	108	83	190	4.74e-51	159
MS.gene33425.t1	MTR_4g013770	68.519	108	34	0	1	108	84	191	1.59e-49	155
MS.gene33425.t1	MTR_4g073950	60.185	108	43	0	1	108	85	192	4.16e-45	144
MS.gene33425.t1	MTR_4g013355	65.741	108	27	1	1	108	82	179	9.92e-45	143
MS.gene33425.t1	MTR_7g093870	56.757	111	45	1	1	108	83	193	3.17e-43	140
MS.gene33425.t1	MTR_7g093820	59.259	108	43	1	2	108	87	194	3.08e-42	137
MS.gene33425.t1	MTR_7g093850	58.333	108	44	1	2	108	87	194	6.40e-42	136
MS.gene33425.t1	MTR_7g093790	56.481	108	46	1	2	108	84	191	2.42e-41	135
MS.gene33425.t1	MTR_7g093830	56.481	108	46	1	2	108	86	193	1.13e-40	133
MS.gene33425.t1	MTR_1g056370	53.704	108	49	1	1	107	78	185	4.05e-37	124
MS.gene33425.t1	MTR_5g096120	51.852	108	51	1	2	108	82	189	7.87e-35	118
MS.gene33425.t1	MTR_3g105640	50.926	108	52	1	2	108	81	188	1.10e-33	115
MS.gene33425.t1	MTR_4g078885	47.222	108	57	0	1	108	78	185	1.97e-32	112
MS.gene33425.t1	MTR_3g034030	47.222	108	57	0	1	108	78	185	1.91e-31	109
MS.gene33425.t1	MTR_3g105630	46.296	108	57	1	2	108	82	189	1.64e-29	104
MS.gene33425.t1	MTR_4g122110	38.889	108	66	0	1	108	73	180	3.13e-21	83.2
MS.gene33425.t1	MTR_4g122130	37.037	108	68	0	1	108	73	180	3.27e-19	78.2
MS.gene33425.t1	MTR_4g049570	32.039	103	70	0	1	103	71	173	1.83e-14	65.9
MS.gene33425.t1	MTR_4g049550	32.039	103	70	0	1	103	71	173	7.21e-14	64.3
MS.gene33425.t1	MTR_8g099115	36.782	87	54	1	1	87	83	168	4.42e-11	57.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33425.t1	AT1G58170	73.148	108	29	0	1	108	78	185	1.16e-55	171
MS.gene33425.t1	AT1G55210	67.593	108	35	0	1	108	80	187	2.73e-49	155
MS.gene33425.t1	AT1G55210	67.593	108	35	0	1	108	80	187	2.73e-49	155
MS.gene33425.t1	AT3G13660	65.741	108	37	0	1	108	18	125	5.56e-48	149
MS.gene33425.t1	AT5G49040	64.815	108	38	0	1	108	84	191	2.65e-46	147
MS.gene33425.t1	AT3G13650	62.037	108	41	0	1	108	79	186	9.75e-44	140
MS.gene33425.t1	AT2G21100	54.206	107	49	0	1	107	81	187	7.08e-40	131
MS.gene33425.t1	AT2G21100	54.206	107	49	0	1	107	81	187	7.08e-40	131
MS.gene33425.t1	AT3G13662	65.263	95	33	0	1	95	78	172	7.66e-39	128
MS.gene33425.t1	AT1G65870	53.271	107	50	0	1	107	83	189	8.59e-39	128
MS.gene33425.t1	AT3G58090	52.336	107	51	0	1	107	165	271	2.15e-37	127
MS.gene33425.t1	AT5G42500	55.238	105	45	1	3	107	83	185	6.69e-36	120
MS.gene33425.t1	AT5G42510	54.286	105	46	1	3	107	80	182	4.61e-35	119
MS.gene33425.t1	AT5G49030	65.591	93	32	0	1	93	84	176	3.06e-34	124
MS.gene33425.t1	AT1G22900	52.336	107	49	1	1	107	89	193	1.43e-33	115
MS.gene33425.t1	AT2G21110	44.860	107	59	0	2	108	80	186	2.28e-29	104
MS.gene33425.t1	AT4G38700	44.643	112	58	2	1	108	79	190	6.55e-28	100

Find 27 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATTCGGGTCCTAAAGTTAA+AGG	0.186513	4.2:-87080587	None:intergenic
ATTGACAACCCTTTAACTTT+AGG	0.265955	4.2:+87080578	MS.gene33425:CDS
TTCATTGAAGGAAAATATAA+TGG	0.268703	4.2:+87080698	MS.gene33425:CDS
TCTGCTTCACAGGAAGAAAT+AGG	0.312713	4.2:+87080650	MS.gene33425:CDS
AACAAGTTTAGAACTCAATT+CGG	0.349877	4.2:-87080603	None:intergenic
GGGAGTACTATTACTATCTT+GGG	0.365410	4.2:+87080719	MS.gene33425:CDS
TGGGAGTACTATTACTATCT+TGG	0.368563	4.2:+87080718	MS.gene33425:CDS
ATGCCTGTGGTTGGTGGTAG+TGG	0.369246	4.2:+87080773	MS.gene33425:CDS
GAGGAATCATGCTCGTAATA+AGG	0.371557	4.2:+87080742	MS.gene33425:CDS
CATTGGATTGATGCCAAAGC+TGG	0.410820	4.2:+87080836	MS.gene33425:CDS
ATTCGGGTCCTAAAGTTAAA+GGG	0.441542	4.2:-87080586	None:intergenic
ATCATGCTCGTAATAAGGTT+AGG	0.442060	4.2:+87080747	MS.gene33425:CDS
AACACAAGCATCCCCAGCTT+TGG	0.447090	4.2:-87080849	None:intergenic
TCATTGAAGGAAAATATAAT+GGG	0.451772	4.2:+87080699	MS.gene33425:CDS
ATGCTCAAGCTAGTACTCAT+TGG	0.460522	4.2:+87080819	MS.gene33425:CDS
GAATTGAGTTCTAAACTTGT+TGG	0.460969	4.2:+87080605	MS.gene33425:CDS
AGGGAGATGCCTGTGGTTGG+TGG	0.465775	4.2:+87080767	MS.gene33425:CDS
AAACTTGTTGGTAGAGCTCA+AGG	0.477359	4.2:+87080617	MS.gene33425:CDS
ACAAGTTTAGAACTCAATTC+GGG	0.477778	4.2:-87080602	None:intergenic
TCATGCTCGTAATAAGGTTA+GGG	0.511498	4.2:+87080748	MS.gene33425:CDS
ATTGGATTGATGCCAAAGCT+GGG	0.519261	4.2:+87080837	MS.gene33425:CDS
GTTAGGGAGATGCCTGTGGT+TGG	0.578353	4.2:+87080764	MS.gene33425:CDS
AGTCCACTACCACCAACCAC+AGG	0.640535	4.2:-87080776	None:intergenic
GTACTATTACTATCTTGGGG+AGG	0.673978	4.2:+87080723	MS.gene33425:CDS
TAAGGTTAGGGAGATGCCTG+TGG	0.677337	4.2:+87080760	MS.gene33425:CDS
GGAGTACTATTACTATCTTG+GGG	0.700411	4.2:+87080720	MS.gene33425:CDS
TTGGATTGATGCCAAAGCTG+GGG	0.710618	4.2:+87080838	MS.gene33425:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TCATTGAAGGAAAATATAAT+GGG	+	chr4.2:87080699-87080718	MS.gene33425:CDS	20.0%
!!	TTCATTGAAGGAAAATATAA+TGG	+	chr4.2:87080698-87080717	MS.gene33425:CDS	20.0%
!!	AACAAGTTTAGAACTCAATT+CGG	-	chr4.2:87080606-87080625	None:intergenic	25.0%
!!	ATGAATTTTGCTTTCATTGA+AGG	+	chr4.2:87080686-87080705	MS.gene33425:CDS	25.0%
!	ACAAGTTTAGAACTCAATTC+GGG	-	chr4.2:87080605-87080624	None:intergenic	30.0%
!	ATTGACAACCCTTTAACTTT+AGG	+	chr4.2:87080578-87080597	MS.gene33425:CDS	30.0%
!!	GAATTGAGTTCTAAACTTGT+TGG	+	chr4.2:87080605-87080624	MS.gene33425:CDS	30.0%
	AATTCGGGTCCTAAAGTTAA+AGG	-	chr4.2:87080590-87080609	None:intergenic	35.0%
	ATCATGCTCGTAATAAGGTT+AGG	+	chr4.2:87080747-87080766	MS.gene33425:CDS	35.0%
	ATTCGGGTCCTAAAGTTAAA+GGG	-	chr4.2:87080589-87080608	None:intergenic	35.0%
	TCATGCTCGTAATAAGGTTA+GGG	+	chr4.2:87080748-87080767	MS.gene33425:CDS	35.0%
!	ATTTTATGCATCTGCTTCAC+AGG	+	chr4.2:87080640-87080659	MS.gene33425:CDS	35.0%
!	GGAGTACTATTACTATCTTG+GGG	+	chr4.2:87080720-87080739	MS.gene33425:CDS	35.0%
!!	GGGAGTACTATTACTATCTT+GGG	+	chr4.2:87080719-87080738	MS.gene33425:CDS	35.0%
!!	TGGGAGTACTATTACTATCT+TGG	+	chr4.2:87080718-87080737	MS.gene33425:CDS	35.0%
	ATGCTCAAGCTAGTACTCAT+TGG	+	chr4.2:87080819-87080838	MS.gene33425:CDS	40.0%
	ATTGGATTGATGCCAAAGCT+GGG	+	chr4.2:87080837-87080856	MS.gene33425:CDS	40.0%
	GAGGAATCATGCTCGTAATA+AGG	+	chr4.2:87080742-87080761	MS.gene33425:CDS	40.0%
	GTACTATTACTATCTTGGGG+AGG	+	chr4.2:87080723-87080742	MS.gene33425:CDS	40.0%
	TCTGCTTCACAGGAAGAAAT+AGG	+	chr4.2:87080650-87080669	MS.gene33425:CDS	40.0%
!!	AAACTTGTTGGTAGAGCTCA+AGG	+	chr4.2:87080617-87080636	MS.gene33425:CDS	40.0%
!!!	GGACTTTTCAGATTTGCTAC+AGG	+	chr4.2:87080794-87080813	MS.gene33425:CDS	40.0%
	CATTGGATTGATGCCAAAGC+TGG	+	chr4.2:87080836-87080855	MS.gene33425:CDS	45.0%
	TTGGATTGATGCCAAAGCTG+GGG	+	chr4.2:87080838-87080857	MS.gene33425:CDS	45.0%
	AACACAAGCATCCCCAGCTT+TGG	-	chr4.2:87080852-87080871	None:intergenic	50.0%
	TAAGGTTAGGGAGATGCCTG+TGG	+	chr4.2:87080760-87080779	MS.gene33425:CDS	50.0%
	AGTCCACTACCACCAACCAC+AGG	-	chr4.2:87080779-87080798	None:intergenic	55.0%
	GTTAGGGAGATGCCTGTGGT+TGG	+	chr4.2:87080764-87080783	MS.gene33425:CDS	55.0%
!!	ATGCCTGTGGTTGGTGGTAG+TGG	+	chr4.2:87080773-87080792	MS.gene33425:CDS	55.0%
	AGGGAGATGCCTGTGGTTGG+TGG	+	chr4.2:87080767-87080786	MS.gene33425:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	87080575	87080901	87080575	ID=MS.gene33425
chr4.2	mRNA	87080575	87080901	87080575	ID=MS.gene33425.t1;Parent=MS.gene33425
chr4.2	exon	87080575	87080901	87080575	ID=MS.gene33425.t1.exon1;Parent=MS.gene33425.t1
chr4.2	CDS	87080575	87080901	87080575	ID=cds.MS.gene33425.t1;Parent=MS.gene33425.t1

Gene Sequence	>MS.gene33425 ATGATTGACAACCCTTTAACTTTAGGACCCGAATTGAGTTCTAAACTTGTTGGTAGAGCTCAAGGATTTTATGCATCTGCTTCACAGGAAGAAATAGGTTTTCTTATGACAATGAATTTTGCTTTCATTGAAGGAAAATATAATGGGAGTACTATTACTATCTTGGGGAGGAATCATGCTCGTAATAAGGTTAGGGAGATGCCTGTGGTTGGTGGTAGTGGACTTTTCAGATTTGCTACAGGATATGCTCAAGCTAGTACTCATTGGATTGATGCCAAAGCTGGGGATGCTTGTGTTGAGTATAATGTTTATGTTTTTCACTATTGA
Protein sequence	>MS.gene33425.t1 MIDNPLTLGPELSSKLVGRAQGFYASASQEEIGFLMTMNFAFIEGKYNGSTITILGRNHARNKVREMPVVGGSGLFRFATGYAQASTHWIDAKAGDACVEYNVYVFHY