Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33710.t1 | PPD89707.1 | 75.4 | 694 | 149 | 2 | 66 | 737 | 24 | 717 | 0.00E+00 | 1104.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33710.t1 | Q7Y1W1 | 56.3 | 490 | 208 | 4 | 1 | 489 | 1 | 485 | 3.6e-169 | 596.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33710.t1 | G7JLT3 | 89.0 | 480 | 50 | 1 | 1 | 480 | 1 | 477 | 1.6e-250 | 875.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052789 | MS.gene33710 | 0.819815 | 9.53E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33710.t1 | MTR_4g014600 | 92.708 | 480 | 32 | 1 | 1 | 480 | 1 | 477 | 0.0 | 884 |
| MS.gene33710.t1 | MTR_4g014600 | 92.640 | 394 | 26 | 1 | 1 | 394 | 1 | 391 | 0.0 | 717 |
| MS.gene33710.t1 | MTR_4g014590 | 100.000 | 263 | 0 | 0 | 479 | 741 | 2 | 264 | 0.0 | 541 |
| MS.gene33710.t1 | MTR_7g069950 | 96.850 | 254 | 8 | 0 | 479 | 732 | 2 | 255 | 1.16e-177 | 508 |
| MS.gene33710.t1 | MTR_5g018940 | 96.800 | 250 | 8 | 0 | 479 | 728 | 2 | 251 | 3.42e-175 | 502 |
| MS.gene33710.t1 | MTR_5g018940 | 96.800 | 250 | 8 | 0 | 479 | 728 | 2 | 251 | 3.42e-175 | 502 |
| MS.gene33710.t1 | MTR_8g052030 | 96.800 | 250 | 8 | 0 | 479 | 728 | 2 | 251 | 3.42e-175 | 502 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33710.t1 | AT5G01590 | 58.907 | 494 | 189 | 7 | 1 | 489 | 1 | 485 | 0.0 | 608 |
| MS.gene33710.t1 | AT2G17360 | 91.506 | 259 | 22 | 0 | 479 | 737 | 2 | 260 | 1.98e-176 | 505 |
| MS.gene33710.t1 | AT5G07090 | 91.506 | 259 | 22 | 0 | 479 | 737 | 2 | 260 | 2.10e-176 | 505 |
| MS.gene33710.t1 | AT5G58420 | 91.506 | 259 | 22 | 0 | 479 | 737 | 2 | 260 | 3.53e-176 | 504 |
| MS.gene33710.t1 | AT5G07090 | 90.909 | 242 | 22 | 0 | 496 | 737 | 1 | 242 | 6.71e-163 | 470 |
| MS.gene33710.t1 | AT5G07090 | 90.909 | 242 | 22 | 0 | 496 | 737 | 1 | 242 | 6.71e-163 | 470 |
| MS.gene33710.t1 | AT2G17360 | 91.667 | 180 | 15 | 0 | 479 | 658 | 2 | 181 | 1.48e-117 | 351 |
Find 225 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTAGTCATGCCCTATTTCTT+TGG | 0.190139 | 4.3:+83752016 | MS.gene33710:CDS |
| GCACCCATCCACATGATTTA+TGG | 0.221444 | 4.3:+83750737 | MS.gene33710:CDS |
| GAAGAATTGTTGGACCATTT+TGG | 0.224308 | 4.3:-83748316 | None:intergenic |
| TGCCGCTGGCCCAAAGAAAT+AGG | 0.281357 | 4.3:-83752026 | None:intergenic |
| ATTGATGTTGCTCAAGCTAT+TGG | 0.282613 | 4.3:+83748750 | MS.gene33710:CDS |
| ATGTAGCGCTAGATAAGTTT+TGG | 0.296534 | 4.3:+83748658 | MS.gene33710:CDS |
| TGTGGGGTGTGTCTGTGGTT+AGG | 0.303432 | 4.3:-83748357 | None:intergenic |
| TGATGCGAAATTTGAGGAAT+TGG | 0.304033 | 4.3:-83748208 | None:intergenic |
| AAGAAAGTTCCGAATATTAC+TGG | 0.305251 | 4.3:+83748594 | MS.gene33710:CDS |
| TAGTCATGCCCTATTTCTTT+GGG | 0.306477 | 4.3:+83752017 | MS.gene33710:CDS |
| CTATCCAGATCCACTAATTA+AGG | 0.316510 | 4.3:+83757376 | MS.gene33710:CDS |
| GGAAGAGGGAGGGGAGTTGG+TGG | 0.323057 | 4.3:-83748159 | None:intergenic |
| GGTTCCGTCTCCACTCTGTT+AGG | 0.323873 | 4.3:+83756701 | MS.gene33710:CDS |
| CTCATCAGAAAGTGTATAAC+TGG | 0.325591 | 4.3:-83748486 | None:intergenic |
| CCACCATGGGTTCCTGTTAA+GGG | 0.326099 | 4.3:+83751883 | MS.gene33710:CDS |
| TTGTCAAGCATCCAATGTTT+TGG | 0.326410 | 4.3:-83756215 | None:intergenic |
| AGGCTCTATGATGTAATTAA+TGG | 0.326946 | 4.3:+83752900 | MS.gene33710:CDS |
| AGGTGAGAGGAGGAAGAGGG+AGG | 0.334097 | 4.3:-83748170 | None:intergenic |
| GACATATCCCGCTGGTTTCA+TGG | 0.336653 | 4.3:+83756508 | MS.gene33710:CDS |
| TTCTTTCACAAACTACAAAA+AGG | 0.338561 | 4.3:+83751856 | MS.gene33710:CDS |
| CCGAATATTACTGGGCTTGA+TGG | 0.342330 | 4.3:+83748603 | MS.gene33710:CDS |
| TTCCAAAGCTCTCTATCTTC+AGG | 0.344377 | 4.3:-83748573 | None:intergenic |
| CAGCGTCATGTTCTTGTTGA+TGG | 0.345107 | 4.3:+83756467 | MS.gene33710:CDS |
| AGGGAGGTGAGAGGAGGAAG+AGG | 0.345181 | 4.3:-83748174 | None:intergenic |
| TCAACCCATTCAACGGAAAC+TGG | 0.347535 | 4.3:+83748121 | MS.gene33710:CDS |
| CACTGAACTGACTACTACTT+TGG | 0.348235 | 4.3:+83752047 | MS.gene33710:CDS |
| GCAGACATACCTAAGATCTT+TGG | 0.354976 | 4.3:-83752100 | None:intergenic |
| GGAGGAAGAGGGAGGGGAGT+TGG | 0.357781 | 4.3:-83748162 | None:intergenic |
| CCATCAAGCCCAGTAATATT+CGG | 0.358656 | 4.3:-83748603 | None:intergenic |
| TTGGGCAATGTGTTTACTAT+TGG | 0.359690 | 4.3:+83757596 | MS.gene33710:CDS |
| GTGAGAGGAGGAAGAGGGAG+GGG | 0.360628 | 4.3:-83748168 | None:intergenic |
| CACGGCGGTGGCGGTGGTGG+TGG | 0.362595 | 4.3:-83748262 | None:intergenic |
| CTTAAAACTGCTTGGGGTGG+TGG | 0.364700 | 4.3:+83750665 | MS.gene33710:CDS |
| AGTACATTCCCAAAGATCTT+AGG | 0.366281 | 4.3:+83752091 | MS.gene33710:CDS |
| GCATACACTTAAAACTGCTT+GGG | 0.372364 | 4.3:+83750658 | MS.gene33710:CDS |
| GTGGGGTGTGTCTGTGGTTA+GGG | 0.373886 | 4.3:-83748356 | None:intergenic |
| GGGAACCAGTTTCCGTTGAA+TGG | 0.379023 | 4.3:-83748126 | None:intergenic |
| GGAATTGGAGGTGTTGGTGG+TGG | 0.380310 | 4.3:-83748241 | None:intergenic |
| TTCCACGGCGGTGGCGGTGG+TGG | 0.381182 | 4.3:-83748265 | None:intergenic |
| ACCACCATGGGTTCCTGTTA+AGG | 0.381373 | 4.3:+83751882 | MS.gene33710:CDS |
| ATTTCTTGTTCCGTTGCATT+TGG | 0.382053 | 4.3:-83748423 | None:intergenic |
| ACCCGGTCCATTCACCAAAA+TGG | 0.383098 | 4.3:+83748302 | MS.gene33710:CDS |
| TCTCTATCTTCAGGCTTATG+AGG | 0.386828 | 4.3:-83748564 | None:intergenic |
| GGGAATGTTGTCATGGTTAC+CGG | 0.387189 | 4.3:+83757464 | MS.gene33710:CDS |
| CACATTCATAGAAGAATTGT+TGG | 0.387563 | 4.3:-83748326 | None:intergenic |
| GTAATGAATGCGAGATTTCC+TGG | 0.388524 | 4.3:+83752945 | MS.gene33710:CDS |
| GCGGATGGTGCGACCATCAT+AGG | 0.388644 | 4.3:-83757354 | None:intergenic |
| GGTGGCGGTGGTGGTGGAAT+TGG | 0.390283 | 4.3:-83748256 | None:intergenic |
| TTAGCCTTAATTAGTGGATC+TGG | 0.390639 | 4.3:-83757380 | None:intergenic |
| GGTGAGAGGAGGAAGAGGGA+GGG | 0.397331 | 4.3:-83748169 | None:intergenic |
| GTTGAATGGGTTGAAGTTGA+TGG | 0.398068 | 4.3:-83748112 | None:intergenic |
| TTGATGTTGCTCAAGCTATT+GGG | 0.399807 | 4.3:+83748751 | MS.gene33710:CDS |
| ATCTTCCACGGCGGTGGCGG+TGG | 0.402097 | 4.3:-83748268 | None:intergenic |
| GAGTGGTGTATGGTTCTATA+AGG | 0.403771 | 4.3:+83750601 | MS.gene33710:CDS |
| ATCTTTGGGAATGTACTTGT+TGG | 0.405106 | 4.3:-83752085 | None:intergenic |
| ACTTCCCATGGCCTCAATCC+TGG | 0.410006 | 4.3:-83752556 | None:intergenic |
| GACTTGTGGGGTCCAGATGA+TGG | 0.410304 | 4.3:-83756359 | None:intergenic |
| CATGAGTTTGCCACTCGGTT+GGG | 0.410419 | 4.3:+83757578 | MS.gene33710:CDS |
| CAGACATACCTAAGATCTTT+GGG | 0.412032 | 4.3:-83752099 | None:intergenic |
| TGCATACACTTAAAACTGCT+TGG | 0.416294 | 4.3:+83750657 | MS.gene33710:CDS |
| TTAAAACTGCTTGGGGTGGT+GGG | 0.417274 | 4.3:+83750666 | MS.gene33710:CDS |
| AGGAATTGGAGAAGTTGAGA+AGG | 0.422609 | 4.3:-83748194 | None:intergenic |
| TGGAGCGTGCAATCGCTACT+TGG | 0.423767 | 4.3:+83750762 | MS.gene33710:CDS |
| GGTGGAATTGGAGGTGTTGG+TGG | 0.424099 | 4.3:-83748244 | None:intergenic |
| AAGTTGGCTAAACTAATTCC+AGG | 0.428800 | 4.3:+83752538 | MS.gene33710:CDS |
| TGGGGTCCAGATGATGGCTT+TGG | 0.432297 | 4.3:-83756353 | None:intergenic |
| GCTAATGATGGCCTCTGGCC+TGG | 0.434082 | 4.3:+83751982 | MS.gene33710:CDS |
| TCTAATATATTTCAGGCAAG+AGG | 0.436290 | 4.3:+83756161 | MS.gene33710:intron |
| TGAGGACTTTGTTGACTTGT+GGG | 0.436322 | 4.3:-83748374 | None:intergenic |
| GGGAGGTGAGAGGAGGAAGA+GGG | 0.436592 | 4.3:-83748173 | None:intergenic |
| TGGCAAAGGCCTTATCCTCC+TGG | 0.436674 | 4.3:+83748720 | MS.gene33710:CDS |
| TTCATCTTCCACGGCGGTGG+CGG | 0.436783 | 4.3:-83748271 | None:intergenic |
| TTTGGTGAATGGACCGGGTT+TGG | 0.439814 | 4.3:-83748298 | None:intergenic |
| GGTGTTGGTGGTGGAGAGAG+AGG | 0.442577 | 4.3:-83748232 | None:intergenic |
| CATTGGATGCTTGACAAACT+TGG | 0.449684 | 4.3:+83756221 | MS.gene33710:CDS |
| TCTTTGGGAATGTACTTGTT+GGG | 0.450292 | 4.3:-83752084 | None:intergenic |
| CATTACATCACCCGCCAGAT+TGG | 0.451225 | 4.3:-83752928 | None:intergenic |
| AATTAATGGCATCGCCAATC+TGG | 0.452261 | 4.3:+83752914 | MS.gene33710:CDS |
| GGAACCAGTTTCCGTTGAAT+GGG | 0.452933 | 4.3:-83748125 | None:intergenic |
| ATCGGCTTCCCAGGAGGATA+AGG | 0.453853 | 4.3:-83748729 | None:intergenic |
| AGAGGTTGATGCGAAATTTG+AGG | 0.455788 | 4.3:-83748214 | None:intergenic |
| TTATCATGAGGAGTGGTGTA+TGG | 0.457939 | 4.3:+83750591 | MS.gene33710:CDS |
| AAGGCACTCCCTTGACTTGT+GGG | 0.458121 | 4.3:-83756372 | None:intergenic |
| ACTGACTACTACTTTGGAAC+AGG | 0.459475 | 4.3:+83752053 | MS.gene33710:CDS |
| AAAGTTCGATCTGTTCAATT+CGG | 0.459883 | 4.3:+83757305 | MS.gene33710:CDS |
| GCCGCTGGCCCAAAGAAATA+GGG | 0.460604 | 4.3:-83752025 | None:intergenic |
| GTGTCATTAGCCTTAATTAG+TGG | 0.466445 | 4.3:-83757386 | None:intergenic |
| CAGGCACCAAAGCCATCATC+TGG | 0.471057 | 4.3:+83756347 | MS.gene33710:intron |
| GGTGGTGGAATTGGAGGTGT+TGG | 0.472723 | 4.3:-83748247 | None:intergenic |
| TCATGAGTTTGCCACTCGGT+TGG | 0.474930 | 4.3:+83757577 | MS.gene33710:CDS |
| CATATTTCAGTCTGTTTCGA+AGG | 0.477511 | 4.3:-83756409 | None:intergenic |
| GGCGGTGGTGGTGGAATTGG+AGG | 0.482953 | 4.3:-83748253 | None:intergenic |
| ACACAGGTCCACCATTCATC+AGG | 0.484693 | 4.3:+83752653 | MS.gene33710:CDS |
| GTCTTCATCTTCCACGGCGG+TGG | 0.484747 | 4.3:-83748274 | None:intergenic |
| CAAGAACAGAGAGAAACATA+AGG | 0.485349 | 4.3:+83757517 | MS.gene33710:CDS |
| CTAAACTAATTCCAGGATTG+AGG | 0.489998 | 4.3:+83752545 | MS.gene33710:CDS |
| ACCATCATAGGTGTTCAGAT+AGG | 0.491470 | 4.3:-83757342 | None:intergenic |
| ATCTGTTCAATTCGGACAGA+AGG | 0.492212 | 4.3:+83757313 | MS.gene33710:CDS |
| ATGGTTCTATAAGGATCGAC+TGG | 0.492481 | 4.3:+83750610 | MS.gene33710:CDS |
| TTCTTGTTGATGGCAAGGTT+AGG | 0.493369 | 4.3:+83756477 | MS.gene33710:CDS |
| CCCTATCTGAACACCTATGA+TGG | 0.494508 | 4.3:+83757341 | MS.gene33710:CDS |
| ACATATCCCGCTGGTTTCAT+GGG | 0.495686 | 4.3:+83756509 | MS.gene33710:CDS |
| TAATTCCAGGATTGAGGCCA+TGG | 0.496414 | 4.3:+83752551 | MS.gene33710:CDS |
| ATGGATCGAATAACATTCAA+TGG | 0.501205 | 4.3:+83752598 | MS.gene33710:CDS |
| GTTCCGTCTCCACTCTGTTA+GGG | 0.502187 | 4.3:+83756702 | MS.gene33710:CDS |
| TTGAGGACTTTGTTGACTTG+TGG | 0.507192 | 4.3:-83748375 | None:intergenic |
| GGAATTGGAGAAGTTGAGAA+GGG | 0.507806 | 4.3:-83748193 | None:intergenic |
| GCATTCCAACGCCTGATGAA+TGG | 0.508612 | 4.3:-83752664 | None:intergenic |
| GTATCTCTTCCCAAGGGAAA+AGG | 0.508790 | 4.3:+83757638 | MS.gene33710:CDS |
| GCAACATCAATCGGCTTCCC+AGG | 0.509357 | 4.3:-83748738 | None:intergenic |
| GGGTCGAACCCGAGGGCCAT+TGG | 0.509929 | 4.3:+83750631 | MS.gene33710:CDS |
| TTGGCAAGGGAACAAAGCCT+TGG | 0.509969 | 4.3:+83757615 | MS.gene33710:CDS |
| GAGACGTGATTTAGCAGTGA+TGG | 0.511406 | 4.3:+83751957 | MS.gene33710:CDS |
| GAAGGCACTCCCTTGACTTG+TGG | 0.511811 | 4.3:-83756373 | None:intergenic |
| CAAGTTGACTGTTATTGAAG+AGG | 0.512862 | 4.3:+83757664 | MS.gene33710:CDS |
| ATAGCTTGAGCAACATCAAT+CGG | 0.513604 | 4.3:-83748747 | None:intergenic |
| GACTCAATGCTCCAAAACAT+TGG | 0.516338 | 4.3:+83756204 | MS.gene33710:CDS |
| TAAAACTGCTTGGGGTGGTG+GGG | 0.516930 | 4.3:+83750667 | MS.gene33710:CDS |
| AGTAGTCAGTTCAGTGCCGC+TGG | 0.518023 | 4.3:-83752040 | None:intergenic |
| ATCAACTTCAACCCATTCAA+CGG | 0.518603 | 4.3:+83748114 | MS.gene33710:CDS |
| TCAGTCTGTTTCGAAGGATG+AGG | 0.518997 | 4.3:-83756403 | None:intergenic |
| CATCCACATGATTTATGGAA+TGG | 0.519518 | 4.3:+83750742 | MS.gene33710:CDS |
| TTTGGTGTCTTCATCTTCCA+CGG | 0.522196 | 4.3:-83748280 | None:intergenic |
| CCATCATAGGTGTTCAGATA+GGG | 0.524783 | 4.3:-83757341 | None:intergenic |
| GGCAAAGGCCTTATCCTCCT+GGG | 0.525192 | 4.3:+83748721 | MS.gene33710:CDS |
| TGCCGAGAAAAGCGCAGTCA+CGG | 0.525298 | 4.3:+83748634 | MS.gene33710:CDS |
| GCTGTGAGATAGACAGAAGT+TGG | 0.526546 | 4.3:+83752522 | MS.gene33710:intron |
| CAATGTGTTTACTATTGGCA+AGG | 0.529290 | 4.3:+83757601 | MS.gene33710:CDS |
| AGCCTGAAGATAGAGAGCTT+TGG | 0.530064 | 4.3:+83748571 | MS.gene33710:CDS |
| GCCCTATTTCTTTGGGCCAG+CGG | 0.535335 | 4.3:+83752024 | MS.gene33710:CDS |
| ATGCAATGCCAATGGCCCTC+GGG | 0.536053 | 4.3:-83750639 | None:intergenic |
| TCATGTTCTTGTTGATGGCA+AGG | 0.537954 | 4.3:+83756472 | MS.gene33710:CDS |
| TGAAGAGGCTAGAAAGAGGC+AGG | 0.540159 | 4.3:+83757679 | MS.gene33710:CDS |
| CATCTGGACCCCACAAGTCA+AGG | 0.541796 | 4.3:+83756363 | MS.gene33710:CDS |
| CTAATATATTTCAGGCAAGA+GGG | 0.544568 | 4.3:+83756162 | MS.gene33710:intron |
| TGGCAAGGGAACAAAGCCTT+GGG | 0.546208 | 4.3:+83757616 | MS.gene33710:CDS |
| CAAAGAAATAGGGCATGACT+AGG | 0.546517 | 4.3:-83752015 | None:intergenic |
| TGGAAGCTAATGATGGCCTC+TGG | 0.547585 | 4.3:+83751977 | MS.gene33710:CDS |
| AATGGCATCGCCAATCTGGC+GGG | 0.552417 | 4.3:+83752918 | MS.gene33710:CDS |
| ACGACCTCTATTCCTTCCTC+CGG | 0.553480 | 4.3:-83757483 | None:intergenic |
| AAGAACAGAGAGAAACATAA+GGG | 0.554158 | 4.3:+83757518 | MS.gene33710:CDS |
| GCCTTGGGTATCTCTTCCCA+AGG | 0.555309 | 4.3:+83757631 | MS.gene33710:CDS |
| TTGAGAAGGGAGGTGAGAGG+AGG | 0.555883 | 4.3:-83748180 | None:intergenic |
| TCAGGCGTTGGAATGCCGAC+AGG | 0.556543 | 4.3:+83752671 | MS.gene33710:CDS |
| TGATGTGGGGAATGTTGTCA+TGG | 0.557070 | 4.3:+83757457 | MS.gene33710:CDS |
| TGACTTGTGGGGTGTGTCTG+TGG | 0.559993 | 4.3:-83748362 | None:intergenic |
| CACCACCACCGCCACCGCCG+TGG | 0.562117 | 4.3:+83748263 | MS.gene33710:CDS |
| GGAGCGTGCAATCGCTACTT+GGG | 0.564345 | 4.3:+83750763 | MS.gene33710:CDS |
| CGTGCAATCGCTACTTGGGA+AGG | 0.564518 | 4.3:+83750767 | MS.gene33710:CDS |
| ATTGGAGAAGTTGAGAAGGG+AGG | 0.564793 | 4.3:-83748190 | None:intergenic |
| TATGCAATGCCAATGGCCCT+CGG | 0.567783 | 4.3:-83750640 | None:intergenic |
| GATCGACTGGGTCGAACCCG+AGG | 0.568670 | 4.3:+83750623 | MS.gene33710:CDS |
| AACATGACGCTGCATCAAGA+TGG | 0.573994 | 4.3:-83756456 | None:intergenic |
| GACCACTTGCATCCTGAACA+TGG | 0.574639 | 4.3:-83757556 | None:intergenic |
| GCAGCTTCAACATGAGGCGG+TGG | 0.574913 | 4.3:+83751927 | MS.gene33710:CDS |
| TGTACGAACCAAGTGGTTCA+CGG | 0.575523 | 4.3:-83752630 | None:intergenic |
| GTCCACCATTCATCAGGCGT+TGG | 0.577924 | 4.3:+83752659 | MS.gene33710:CDS |
| CATTCCATAAATCATGTGGA+TGG | 0.578067 | 4.3:-83750741 | None:intergenic |
| TAATGGCATCGCCAATCTGG+CGG | 0.578144 | 4.3:+83752917 | MS.gene33710:CDS |
| AGAAAGTTCCGAATATTACT+GGG | 0.578755 | 4.3:+83748595 | MS.gene33710:CDS |
| TGACTAGGAATTCTAACTCC+AGG | 0.578909 | 4.3:-83752000 | None:intergenic |
| CACATACCCATGAAACCAGC+GGG | 0.579767 | 4.3:-83756515 | None:intergenic |
| TGTTAGGGATGATGAGGCAA+AGG | 0.579828 | 4.3:+83756717 | MS.gene33710:CDS |
| TTTCGAAGGATGAGGATCAA+AGG | 0.580618 | 4.3:-83756395 | None:intergenic |
| GTGGAGACGGAACCGACCCT+TGG | 0.581652 | 4.3:-83756692 | None:intergenic |
| TTGTCATGGTTACCGGAGGA+AGG | 0.582711 | 4.3:+83757471 | MS.gene33710:CDS |
| TGGATGCTTGACAAACTTGG+TGG | 0.583185 | 4.3:+83756224 | MS.gene33710:CDS |
| CATCCCTAACAGAGTGGAGA+CGG | 0.583367 | 4.3:-83756705 | None:intergenic |
| TCTGAGCATAGAACAAGCAA+TGG | 0.583742 | 4.3:+83752579 | MS.gene33710:CDS |
| GATTTAGTTATCATGAGGAG+TGG | 0.591381 | 4.3:+83750584 | MS.gene33710:intron |
| TCTATGCTCAGAACTTCCCA+TGG | 0.595336 | 4.3:-83752568 | None:intergenic |
| ATCTGGACCCCACAAGTCAA+GGG | 0.597269 | 4.3:+83756364 | MS.gene33710:CDS |
| AAGTTGAGAAGGGAGGTGAG+AGG | 0.597273 | 4.3:-83748183 | None:intergenic |
| TCTGTTCAATTCGGACAGAA+GGG | 0.598687 | 4.3:+83757314 | MS.gene33710:CDS |
| CCACTCTGTTAGGGATGATG+AGG | 0.598797 | 4.3:+83756711 | MS.gene33710:CDS |
| AGGCACTCCCTTGACTTGTG+GGG | 0.598802 | 4.3:-83756371 | None:intergenic |
| GAAGATGAAGACACCAAACC+CGG | 0.600825 | 4.3:+83748285 | MS.gene33710:CDS |
| GTTACCGGAGGAAGGAATAG+AGG | 0.602203 | 4.3:+83757479 | MS.gene33710:CDS |
| GAATGGTACCGTGAACCACT+TGG | 0.603216 | 4.3:+83752622 | MS.gene33710:CDS |
| AGCTGATAAGATGAATGAGT+GGG | 0.603762 | 4.3:+83748521 | MS.gene33710:CDS |
| CGTCTTCTGTATGACACCAA+GGG | 0.605611 | 4.3:+83756676 | MS.gene33710:CDS |
| TTATTGAAGAGGCTAGAAAG+AGG | 0.605630 | 4.3:+83757675 | MS.gene33710:CDS |
| CCGTCTTCTGTATGACACCA+AGG | 0.606098 | 4.3:+83756675 | MS.gene33710:CDS |
| GCAGTGATGGAAGCTAATGA+TGG | 0.607053 | 4.3:+83751970 | MS.gene33710:CDS |
| TTAAGTGTATGCAATGCCAA+TGG | 0.608483 | 4.3:-83750647 | None:intergenic |
| CCTTGGTGTCATACAGAAGA+CGG | 0.610727 | 4.3:-83756675 | None:intergenic |
| ATTCCATAAATCATGTGGAT+GGG | 0.612495 | 4.3:-83750740 | None:intergenic |
| AATGTGTTTACTATTGGCAA+GGG | 0.614045 | 4.3:+83757602 | MS.gene33710:CDS |
| GAATAACATTCAATGGCGAA+TGG | 0.615395 | 4.3:+83752605 | MS.gene33710:CDS |
| TATTTGATTTAGTTATCATG+AGG | 0.616799 | 4.3:+83750579 | MS.gene33710:intron |
| GGAAGGAATAGAGGTCGTGT+TGG | 0.618743 | 4.3:+83757488 | MS.gene33710:CDS |
| GTGGTCATGAGTTTGCCACT+CGG | 0.619011 | 4.3:+83757573 | MS.gene33710:CDS |
| TTCCAACGCCTGATGAATGG+TGG | 0.620251 | 4.3:-83752661 | None:intergenic |
| CATACACTTAAAACTGCTTG+GGG | 0.623287 | 4.3:+83750659 | MS.gene33710:CDS |
| TAGCTGATAAGATGAATGAG+TGG | 0.625483 | 4.3:+83748520 | MS.gene33710:CDS |
| GCGTTGGAATGCCGACAGGA+TGG | 0.626966 | 4.3:+83752675 | MS.gene33710:CDS |
| CGAAGCTAAACCAAATGCAA+CGG | 0.628654 | 4.3:+83748413 | MS.gene33710:CDS |
| TTCTGTATGACACCAAGGGT+CGG | 0.629625 | 4.3:+83756680 | MS.gene33710:CDS |
| TAGTGGATCTGGATAGCGGA+TGG | 0.630246 | 4.3:-83757369 | None:intergenic |
| GCTCCATTCCATAAATCATG+TGG | 0.634373 | 4.3:-83750745 | None:intergenic |
| TTATAAGCAGCTTCAACATG+AGG | 0.638676 | 4.3:+83751921 | MS.gene33710:CDS |
| ATCGACTGGGTCGAACCCGA+GGG | 0.641942 | 4.3:+83750624 | MS.gene33710:CDS |
| GAAGGATGAGGATCAAAGGA+AGG | 0.642486 | 4.3:-83756391 | None:intergenic |
| CCCTTAACAGGAACCCATGG+TGG | 0.643315 | 4.3:-83751883 | None:intergenic |
| AATTCCAGGATTGAGGCCAT+GGG | 0.643550 | 4.3:+83752552 | MS.gene33710:CDS |
| TAATTAGTGGATCTGGATAG+CGG | 0.647434 | 4.3:-83757373 | None:intergenic |
| ATCCATGTTCAGGATGCAAG+TGG | 0.648183 | 4.3:+83757554 | MS.gene33710:CDS |
| AATCCCTTAACAGGAACCCA+TGG | 0.648355 | 4.3:-83751886 | None:intergenic |
| ACACTTAAAACTGCTTGGGG+TGG | 0.657410 | 4.3:+83750662 | MS.gene33710:CDS |
| CCCTTGGGAAGAGATACCCA+AGG | 0.659442 | 4.3:-83757632 | None:intergenic |
| TAAGCAGCTTCAACATGAGG+CGG | 0.663272 | 4.3:+83751924 | MS.gene33710:CDS |
| GAGGACTTTGTTGACTTGTG+GGG | 0.663696 | 4.3:-83748373 | None:intergenic |
| CCTCATCATCCCTAACAGAG+TGG | 0.666127 | 4.3:-83756711 | None:intergenic |
| GAATTCTAACTCCAGGCCAG+AGG | 0.668583 | 4.3:-83751993 | None:intergenic |
| AGACACACTTACCATCCTGT+CGG | 0.669599 | 4.3:-83752686 | None:intergenic |
| TGGTTCTATAAGGATCGACT+GGG | 0.676661 | 4.3:+83750611 | MS.gene33710:CDS |
| ACATCAATCGGCTTCCCAGG+AGG | 0.676929 | 4.3:-83748735 | None:intergenic |
| CCTTGGGTATCTCTTCCCAA+GGG | 0.683663 | 4.3:+83757632 | MS.gene33710:CDS |
| ACAGACAAGACATATCCCGC+TGG | 0.683859 | 4.3:+83756500 | MS.gene33710:CDS |
| CCACTTGGTTCGTACAACAC+AGG | 0.683947 | 4.3:+83752637 | MS.gene33710:CDS |
| AATGTTGTCATGGTTACCGG+AGG | 0.684540 | 4.3:+83757467 | MS.gene33710:CDS |
| GCACATACCCATGAAACCAG+CGG | 0.695365 | 4.3:-83756516 | None:intergenic |
| AAACACATTGCCCAACCGAG+TGG | 0.706925 | 4.3:-83757588 | None:intergenic |
| GGTGTCTTCATCTTCCACGG+CGG | 0.739316 | 4.3:-83748277 | None:intergenic |
| CCTGTGTTGTACGAACCAAG+TGG | 0.802878 | 4.3:-83752637 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 83748105 | 83757727 | 83748105 | ID=MS.gene33710 |
| chr4.3 | mRNA | 83748105 | 83757727 | 83748105 | ID=MS.gene33710.t1;Parent=MS.gene33710 |
| chr4.3 | exon | 83748105 | 83748804 | 83748105 | ID=MS.gene33710.t1.exon1;Parent=MS.gene33710.t1 |
| chr4.3 | CDS | 83748105 | 83748804 | 83748105 | ID=cds.MS.gene33710.t1;Parent=MS.gene33710.t1 |
| chr4.3 | exon | 83750591 | 83750788 | 83750591 | ID=MS.gene33710.t1.exon2;Parent=MS.gene33710.t1 |
| chr4.3 | CDS | 83750591 | 83750788 | 83750591 | ID=cds.MS.gene33710.t1;Parent=MS.gene33710.t1 |
| chr4.3 | exon | 83751830 | 83752112 | 83751830 | ID=MS.gene33710.t1.exon3;Parent=MS.gene33710.t1 |
| chr4.3 | CDS | 83751830 | 83752112 | 83751830 | ID=cds.MS.gene33710.t1;Parent=MS.gene33710.t1 |
| chr4.3 | exon | 83752534 | 83752696 | 83752534 | ID=MS.gene33710.t1.exon4;Parent=MS.gene33710.t1 |
| chr4.3 | CDS | 83752534 | 83752696 | 83752534 | ID=cds.MS.gene33710.t1;Parent=MS.gene33710.t1 |
| chr4.3 | exon | 83752900 | 83752989 | 83752900 | ID=MS.gene33710.t1.exon5;Parent=MS.gene33710.t1 |
| chr4.3 | CDS | 83752900 | 83752989 | 83752900 | ID=cds.MS.gene33710.t1;Parent=MS.gene33710.t1 |
| chr4.3 | exon | 83756176 | 83756253 | 83756176 | ID=MS.gene33710.t1.exon6;Parent=MS.gene33710.t1 |
| chr4.3 | CDS | 83756176 | 83756253 | 83756176 | ID=cds.MS.gene33710.t1;Parent=MS.gene33710.t1 |
| chr4.3 | exon | 83756350 | 83756530 | 83756350 | ID=MS.gene33710.t1.exon7;Parent=MS.gene33710.t1 |
| chr4.3 | CDS | 83756350 | 83756530 | 83756350 | ID=cds.MS.gene33710.t1;Parent=MS.gene33710.t1 |
| chr4.3 | exon | 83756641 | 83756738 | 83756641 | ID=MS.gene33710.t1.exon8;Parent=MS.gene33710.t1 |
| chr4.3 | CDS | 83756641 | 83756738 | 83756641 | ID=cds.MS.gene33710.t1;Parent=MS.gene33710.t1 |
| chr4.3 | exon | 83757293 | 83757727 | 83757293 | ID=MS.gene33710.t1.exon9;Parent=MS.gene33710.t1 |
| chr4.3 | CDS | 83757293 | 83757727 | 83757293 | ID=cds.MS.gene33710.t1;Parent=MS.gene33710.t1 |
| Gene Sequence |
| Protein sequence |
