Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33774.t1 | XP_013457066.1 | 97.2 | 107 | 3 | 0 | 1 | 107 | 1 | 107 | 6.60E-55 | 223 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33774.t1 | Q9SKU1 | 40.2 | 102 | 50 | 4 | 14 | 107 | 18 | 116 | 9.4e-10 | 64.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33774.t1 | G7ZZ83 | 97.2 | 107 | 3 | 0 | 1 | 107 | 1 | 107 | 4.7e-55 | 223.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050894 | MS.gene33774 | 0.808233 | 3.43E-50 | -1.69E-46 |
| MS.gene052494 | MS.gene33774 | 0.813681 | 2.26E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33774.t1 | MTR_4g088980 | 97.196 | 107 | 3 | 0 | 1 | 107 | 1 | 107 | 1.78e-73 | 220 |
| MS.gene33774.t1 | MTR_2g013220 | 55.882 | 102 | 41 | 3 | 9 | 107 | 21 | 121 | 6.24e-26 | 98.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 42 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGGAGGAAGGTAACGTTCTT+AGG | 0.196876 | 4.3:+23909171 | MS.gene33774:CDS |
| CCGAATACATCCGGAGATTC+CGG | 0.326239 | 4.3:-23909005 | None:intergenic |
| GGCGACGACGACAACTATTC+TGG | 0.330828 | 4.3:+23908921 | MS.gene33774:CDS |
| ATGGAAAACGGAAACGGTTA+TGG | 0.368793 | 4.3:+23909083 | MS.gene33774:CDS |
| CCGGAATCTCCGGATGTATT+CGG | 0.406444 | 4.3:+23909005 | MS.gene33774:CDS |
| GCTGCTGTTGTGTGATCTAC+AGG | 0.409676 | 4.3:-23908981 | None:intergenic |
| AACAGCAGCGCCGGAATCTC+CGG | 0.421407 | 4.3:+23908995 | MS.gene33774:CDS |
| GCTTCCTCCTCCTGCTGAGA+TGG | 0.448243 | 4.3:+23909145 | MS.gene33774:CDS |
| GGTGAAGTGGTCTTCGTTGA+CGG | 0.458970 | 4.3:-23908895 | None:intergenic |
| CTAAGAACGTTACCTTCCTC+CGG | 0.484104 | 4.3:-23909170 | None:intergenic |
| AGAATAGTTGTCGTCGTCGC+CGG | 0.485975 | 4.3:-23908919 | None:intergenic |
| AGGAGGAAGCACCGGACCAT+CGG | 0.488490 | 4.3:-23909132 | None:intergenic |
| CATCACATGCACTGGTTCGA+AGG | 0.488969 | 4.3:-23909063 | None:intergenic |
| GTGATCTACAGGGACATCTT+CGG | 0.492594 | 4.3:-23908970 | None:intergenic |
| CCAGTGCATGTGATGGAAAA+CGG | 0.493151 | 4.3:+23909071 | MS.gene33774:CDS |
| TCCTCCGGCTCCATCTCAGC+AGG | 0.495243 | 4.3:-23909155 | None:intergenic |
| GGAGGAAGGTAACGTTCTTA+GGG | 0.497743 | 4.3:+23909172 | MS.gene33774:CDS |
| TCCTCCTGCTGAGATGGAGC+CGG | 0.501036 | 4.3:+23909151 | MS.gene33774:CDS |
| TGTATTCGGATTCAGCGATC+AGG | 0.515580 | 4.3:+23909019 | MS.gene33774:CDS |
| CGTCGTCGCCGGCGGTGAAG+TGG | 0.522107 | 4.3:-23908908 | None:intergenic |
| GGCTCCATCTCAGCAGGAGG+AGG | 0.522435 | 4.3:-23909149 | None:intergenic |
| GACGACAACTATTCTGGATA+CGG | 0.532542 | 4.3:+23908927 | MS.gene33774:CDS |
| AAGGGAGACTGAGCGTAACT+CGG | 0.540887 | 4.3:-23909044 | None:intergenic |
| AAGGTAACGTTCTTAGGGAA+TGG | 0.547512 | 4.3:+23909177 | MS.gene33774:CDS |
| AACGAAGACCACTTCACCGC+CGG | 0.548831 | 4.3:+23908900 | MS.gene33774:CDS |
| TCGCTGAATCCGAATACATC+CGG | 0.554031 | 4.3:-23909014 | None:intergenic |
| CTTCGAACCAGTGCATGTGA+TGG | 0.556019 | 4.3:+23909064 | MS.gene33774:CDS |
| GTGGTCTTCGTTGACGGTGA+AGG | 0.559914 | 4.3:-23908889 | None:intergenic |
| TCCGGCTCCATCTCAGCAGG+AGG | 0.569202 | 4.3:-23909152 | None:intergenic |
| TTCGTTGACGGTGAAGGACA+TGG | 0.571845 | 4.3:-23908883 | None:intergenic |
| CATGTGATGGAAAACGGAAA+CGG | 0.572497 | 4.3:+23909077 | MS.gene33774:CDS |
| ATCACATGCACTGGTTCGAA+GGG | 0.575990 | 4.3:-23909062 | None:intergenic |
| AGATCACACAACAGCAGCGC+CGG | 0.576259 | 4.3:+23908986 | MS.gene33774:CDS |
| GCTGAGATGGAGCCGGAGGA+AGG | 0.577970 | 4.3:+23909158 | MS.gene33774:CDS |
| TCCTGCTGAGATGGAGCCGG+AGG | 0.580872 | 4.3:+23909154 | MS.gene33774:CDS |
| TCAGCAGGAGGAGGAAGCAC+CGG | 0.587615 | 4.3:-23909140 | None:intergenic |
| ATAGTTGTCGTCGTCGCCGG+CGG | 0.604799 | 4.3:-23908916 | None:intergenic |
| CTGCTGTTGTGTGATCTACA+GGG | 0.636206 | 4.3:-23908980 | None:intergenic |
| GACAACTATTCTGGATACGG+CGG | 0.653119 | 4.3:+23908930 | MS.gene33774:CDS |
| ACGTTCTTAGGGAATGGCGC+CGG | 0.656812 | 4.3:+23909183 | MS.gene33774:CDS |
| ATCTACAGGGACATCTTCGG+CGG | 0.664893 | 4.3:-23908967 | None:intergenic |
| GAAAACGGAAACGGTTATGG+CGG | 0.686022 | 4.3:+23909086 | MS.gene33774:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGGTAACGTTCTTAGGGAA+TGG | + | chr4.3:23909177-23909196 | MS.gene33774:CDS | 40.0% | |
| ATGGAAAACGGAAACGGTTA+TGG | + | chr4.3:23909083-23909102 | MS.gene33774:CDS | 40.0% | |
| GACGACAACTATTCTGGATA+CGG | + | chr4.3:23908927-23908946 | MS.gene33774:CDS | 40.0% | |
| ! | CATGTGATGGAAAACGGAAA+CGG | + | chr4.3:23909077-23909096 | MS.gene33774:CDS | 40.0% |
| CTAAGAACGTTACCTTCCTC+CGG | - | chr4.3:23909173-23909192 | None:intergenic | 45.0% | |
| CTGCTGTTGTGTGATCTACA+GGG | - | chr4.3:23908983-23909002 | None:intergenic | 45.0% | |
| GAAAACGGAAACGGTTATGG+CGG | + | chr4.3:23909086-23909105 | MS.gene33774:CDS | 45.0% | |
| GACAACTATTCTGGATACGG+CGG | + | chr4.3:23908930-23908949 | MS.gene33774:CDS | 45.0% | |
| GGAGGAAGGTAACGTTCTTA+GGG | + | chr4.3:23909172-23909191 | MS.gene33774:CDS | 45.0% | |
| GTGATCTACAGGGACATCTT+CGG | - | chr4.3:23908973-23908992 | None:intergenic | 45.0% | |
| TCGCTGAATCCGAATACATC+CGG | - | chr4.3:23909017-23909036 | None:intergenic | 45.0% | |
| TGTATTCGGATTCAGCGATC+AGG | + | chr4.3:23909019-23909038 | MS.gene33774:CDS | 45.0% | |
| ! | CCAGTGCATGTGATGGAAAA+CGG | + | chr4.3:23909071-23909090 | MS.gene33774:CDS | 45.0% |
| !! | ATCACATGCACTGGTTCGAA+GGG | - | chr4.3:23909065-23909084 | None:intergenic | 45.0% |
| AAGGGAGACTGAGCGTAACT+CGG | - | chr4.3:23909047-23909066 | None:intergenic | 50.0% | |
| AGAATAGTTGTCGTCGTCGC+CGG | - | chr4.3:23908922-23908941 | None:intergenic | 50.0% | |
| ATCTACAGGGACATCTTCGG+CGG | - | chr4.3:23908970-23908989 | None:intergenic | 50.0% | |
| CCGAATACATCCGGAGATTC+CGG | - | chr4.3:23909008-23909027 | None:intergenic | 50.0% | |
| CCGGAATCTCCGGATGTATT+CGG | + | chr4.3:23909005-23909024 | MS.gene33774:CDS | 50.0% | |
| CGGAGGAAGGTAACGTTCTT+AGG | + | chr4.3:23909171-23909190 | MS.gene33774:CDS | 50.0% | |
| CTTCGAACCAGTGCATGTGA+TGG | + | chr4.3:23909064-23909083 | MS.gene33774:CDS | 50.0% | |
| GCTGCTGTTGTGTGATCTAC+AGG | - | chr4.3:23908984-23909003 | None:intergenic | 50.0% | |
| GGTGAAGTGGTCTTCGTTGA+CGG | - | chr4.3:23908898-23908917 | None:intergenic | 50.0% | |
| ! | CCGTTTTCCATCACATGCAC+TGG | - | chr4.3:23909074-23909093 | None:intergenic | 50.0% |
| ! | CGTTTTTGTCTCCGATGGTC+CGG | + | chr4.3:23909121-23909140 | MS.gene33774:CDS | 50.0% |
| ! | GACGACGTTTTTGTCTCCGA+TGG | + | chr4.3:23909116-23909135 | MS.gene33774:CDS | 50.0% |
| !! | CATCACATGCACTGGTTCGA+AGG | - | chr4.3:23909066-23909085 | None:intergenic | 50.0% |
| AACGAAGACCACTTCACCGC+CGG | + | chr4.3:23908900-23908919 | MS.gene33774:CDS | 55.0% | |
| ACGTTCTTAGGGAATGGCGC+CGG | + | chr4.3:23909183-23909202 | MS.gene33774:CDS | 55.0% | |
| AGATCACACAACAGCAGCGC+CGG | + | chr4.3:23908986-23909005 | MS.gene33774:CDS | 55.0% | |
| GGCGACGACGACAACTATTC+TGG | + | chr4.3:23908921-23908940 | MS.gene33774:CDS | 55.0% | |
| GTGGTCTTCGTTGACGGTGA+AGG | - | chr4.3:23908892-23908911 | None:intergenic | 55.0% | |
| ATAGTTGTCGTCGTCGCCGG+CGG | - | chr4.3:23908919-23908938 | None:intergenic | 60.0% | |
| GCTTCCTCCTCCTGCTGAGA+TGG | + | chr4.3:23909145-23909164 | MS.gene33774:CDS | 60.0% | |
| TCCTCCTGCTGAGATGGAGC+CGG | + | chr4.3:23909151-23909170 | MS.gene33774:CDS | 60.0% | |
| ! | AACAGCAGCGCCGGAATCTC+CGG | + | chr4.3:23908995-23909014 | MS.gene33774:CDS | 60.0% |
| ! | TCAGCAGGAGGAGGAAGCAC+CGG | - | chr4.3:23909143-23909162 | None:intergenic | 60.0% |
| !! | AGGAGGAAGCACCGGACCAT+CGG | - | chr4.3:23909135-23909154 | None:intergenic | 60.0% |
| GCTGAGATGGAGCCGGAGGA+AGG | + | chr4.3:23909158-23909177 | MS.gene33774:CDS | 65.0% | |
| GGCTCCATCTCAGCAGGAGG+AGG | - | chr4.3:23909152-23909171 | None:intergenic | 65.0% | |
| TCCGGCTCCATCTCAGCAGG+AGG | - | chr4.3:23909155-23909174 | None:intergenic | 65.0% | |
| TCCTCCGGCTCCATCTCAGC+AGG | - | chr4.3:23909158-23909177 | None:intergenic | 65.0% | |
| TCCTGCTGAGATGGAGCCGG+AGG | + | chr4.3:23909154-23909173 | MS.gene33774:CDS | 65.0% | |
| CGTCGTCGCCGGCGGTGAAG+TGG | - | chr4.3:23908911-23908930 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 23908885 | 23909208 | 23908885 | ID=MS.gene33774 |
| chr4.3 | mRNA | 23908885 | 23909208 | 23908885 | ID=MS.gene33774.t1;Parent=MS.gene33774 |
| chr4.3 | exon | 23908885 | 23909208 | 23908885 | ID=MS.gene33774.t1.exon1;Parent=MS.gene33774.t1 |
| chr4.3 | CDS | 23908885 | 23909208 | 23908885 | ID=cds.MS.gene33774.t1;Parent=MS.gene33774.t1 |
| Gene Sequence |
| Protein sequence |
