Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33775.t1 | XP_013457066.1 | 89.1 | 184 | 19 | 1 | 1 | 183 | 123 | 306 | 3.10E-73 | 284.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33775.t1 | Q7XKE9 | 69.9 | 123 | 36 | 1 | 1 | 123 | 133 | 254 | 1.1e-42 | 174.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33775.t1 | G7ZZ83 | 89.1 | 184 | 19 | 1 | 1 | 183 | 123 | 306 | 2.3e-73 | 284.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050838 | MS.gene33775 | 0.800815 | 1.21E-48 | -1.69E-46 |
| MS.gene058720 | MS.gene33775 | 0.801643 | 8.17E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33775.t1 | MTR_4g088980 | 97.283 | 184 | 4 | 1 | 1 | 183 | 123 | 306 | 1.26e-126 | 358 |
| MS.gene33775.t1 | MTR_2g013220 | 74.457 | 184 | 39 | 2 | 1 | 183 | 137 | 313 | 2.64e-84 | 251 |
| MS.gene33775.t1 | MTR_8g098515 | 58.673 | 196 | 68 | 4 | 1 | 183 | 151 | 346 | 5.18e-57 | 183 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33775.t1 | AT2G20760 | 56.477 | 193 | 68 | 4 | 1 | 179 | 132 | 322 | 3.10e-54 | 175 |
| MS.gene33775.t1 | AT2G40060 | 63.158 | 114 | 40 | 1 | 4 | 115 | 115 | 228 | 8.19e-47 | 154 |
| MS.gene33775.t1 | AT3G51890 | 60.163 | 123 | 46 | 1 | 1 | 123 | 121 | 240 | 1.04e-45 | 151 |
Find 58 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCATCCTTTGGTTGTGATTC+AGG | 0.112857 | 4.3:-23911692 | None:intergenic |
| GTTTGCCTGGCTTTGGGCCT+TGG | 0.150519 | 4.3:-23911490 | None:intergenic |
| CGGTGGGTTTGCCTGGCTTT+GGG | 0.248540 | 4.3:-23911496 | None:intergenic |
| GCAAACCCACCGACCTTTCT+AGG | 0.257216 | 4.3:+23911507 | MS.gene33775:CDS |
| TCGGTGGGTTTGCCTGGCTT+TGG | 0.260717 | 4.3:-23911497 | None:intergenic |
| TTTGTGAAAATTCTCTTGAT+TGG | 0.330165 | 4.3:-23911351 | None:intergenic |
| TGAATCTACAGTACCGTTAT+TGG | 0.331003 | 4.3:-23911717 | None:intergenic |
| GGTGCTGATCCATTTGGTTT+AGG | 0.333375 | 4.3:-23911659 | None:intergenic |
| TGAGAAGAAAAGAGGCAAAA+AGG | 0.365407 | 4.3:+23911436 | MS.gene33775:CDS |
| AAAATTACTGGAAAGCAATT+GGG | 0.366531 | 4.3:+23911384 | MS.gene33775:CDS |
| TGCCTGGCTTTGGGCCTTGG+AGG | 0.380338 | 4.3:-23911487 | None:intergenic |
| TTCTCTTTGGAGTCTTTAGC+AGG | 0.392502 | 4.3:-23911596 | None:intergenic |
| CCGTTATTGGCAGCATCCTT+TGG | 0.394987 | 4.3:-23911704 | None:intergenic |
| GGTTTAGGTGCTGATCCATT+TGG | 0.396493 | 4.3:-23911644 | None:intergenic |
| GAAAGGTCGGTGGGTTTGCC+TGG | 0.402706 | 4.3:-23911503 | None:intergenic |
| TTGGAGTCTTTAGCAGGTGC+AGG | 0.408406 | 4.3:-23911590 | None:intergenic |
| ACTTCCGGTGCTGATCCATT+TGG | 0.420411 | 4.3:-23911665 | None:intergenic |
| TGTGATTCAGGTGCAACTTC+CGG | 0.424313 | 4.3:-23911680 | None:intergenic |
| CTCAATGTTGGCAACCTCAC+GGG | 0.426203 | 4.3:-23911417 | None:intergenic |
| GCAGACTGCTCCTCAGTGGC+AGG | 0.431231 | 4.3:-23911755 | None:intergenic |
| GTTTGTGGAACAGTTATACT+TGG | 0.453267 | 4.3:+23911328 | MS.gene33775:intron |
| ACAAGGTTCAAAACAGAGAA+AGG | 0.459770 | 4.3:+23911217 | MS.gene33775:CDS |
| AATGAGATTAAAGATTATTG+AGG | 0.464614 | 4.3:+23911137 | None:intergenic |
| GAAACAGCAACTAAACCAAA+TGG | 0.475248 | 4.3:+23911629 | MS.gene33775:CDS |
| GGATCAGCACCTAAACCAAA+TGG | 0.488856 | 4.3:+23911650 | MS.gene33775:CDS |
| AAGTGGGTTTCTATGAGAAA+AGG | 0.493819 | 4.3:+23911175 | MS.gene33775:CDS |
| GTGGCAGGAGCTTCTTTCTG+AGG | 0.503664 | 4.3:-23911740 | None:intergenic |
| GTCCTCCAAGGCCCAAAGCC+AGG | 0.509150 | 4.3:+23911485 | MS.gene33775:CDS |
| TTAGCAGGTGCAGGTGGAGG+AGG | 0.510354 | 4.3:-23911581 | None:intergenic |
| CCAAAGGATGCTGCCAATAA+CGG | 0.510873 | 4.3:+23911704 | MS.gene33775:CDS |
| ATATCTGCCGCATCCTAGAA+AGG | 0.513686 | 4.3:-23911520 | None:intergenic |
| TCTTTAGCAGGTGCAGGTGG+AGG | 0.524857 | 4.3:-23911584 | None:intergenic |
| CAAGGTTCAAAACAGAGAAA+GGG | 0.537446 | 4.3:+23911218 | MS.gene33775:CDS |
| TCTCAATGTTGGCAACCTCA+CGG | 0.539692 | 4.3:-23911418 | None:intergenic |
| GAGTCTTTAGCAGGTGCAGG+TGG | 0.551285 | 4.3:-23911587 | None:intergenic |
| GCCAACATTGAGAAGAAAAG+AGG | 0.561154 | 4.3:+23911428 | MS.gene33775:CDS |
| AAAGATTATTGAGGAAGCTG+AGG | 0.562392 | 4.3:+23911146 | MS.gene33775:CDS |
| GAAGCTGAGGATTACAAAGT+GGG | 0.570600 | 4.3:+23911159 | MS.gene33775:CDS |
| AAATTACTGGAAAGCAATTG+GGG | 0.589625 | 4.3:+23911385 | MS.gene33775:CDS |
| CCTTGGAGGACAGTAACTGA+TGG | 0.599991 | 4.3:-23911473 | None:intergenic |
| CCACCGACCTTTCTAGGATG+CGG | 0.602155 | 4.3:+23911513 | MS.gene33775:CDS |
| TGCTAAAGACTCCAAAGAGA+AGG | 0.604267 | 4.3:+23911598 | MS.gene33775:CDS |
| TGCACCTGAATCACAACCAA+AGG | 0.604455 | 4.3:+23911688 | MS.gene33775:CDS |
| GGAGGAGGAATCATGTGAAG+TGG | 0.606489 | 4.3:-23911566 | None:intergenic |
| CTGCCGCATCCTAGAAAGGT+CGG | 0.609710 | 4.3:-23911516 | None:intergenic |
| GGAAGCTGAGGATTACAAAG+TGG | 0.610216 | 4.3:+23911158 | MS.gene33775:CDS |
| CGCATCCTAGAAAGGTCGGT+GGG | 0.610689 | 4.3:-23911512 | None:intergenic |
| TCAATGTTGGCAACCTCACG+GGG | 0.611587 | 4.3:-23911416 | None:intergenic |
| TCAAAACAGAGAAAGGGAGA+AGG | 0.613489 | 4.3:+23911224 | MS.gene33775:CDS |
| CCATCAGTTACTGTCCTCCA+AGG | 0.615544 | 4.3:+23911473 | MS.gene33775:CDS |
| CCGCATCCTAGAAAGGTCGG+TGG | 0.616621 | 4.3:-23911513 | None:intergenic |
| TAAACCAAATGGATCAGCAC+CGG | 0.618396 | 4.3:+23911661 | MS.gene33775:CDS |
| GGAGGAATCATGTGAAGTGG+TGG | 0.643969 | 4.3:-23911563 | None:intergenic |
| AAAGACTCCAAAGAGAAGGA+CGG | 0.661177 | 4.3:+23911602 | MS.gene33775:CDS |
| GAAAGAAGCTCCTGCCACTG+AGG | 0.676516 | 4.3:+23911745 | MS.gene33775:CDS |
| TGCAGCAGACTGCTCCTCAG+TGG | 0.680257 | 4.3:-23911759 | None:intergenic |
| TGGGGAGATAATTCCCCGTG+AGG | 0.688947 | 4.3:+23911403 | MS.gene33775:CDS |
| GCTCAATGTTGAGACAAACA+AGG | 0.723788 | 4.3:+23911200 | MS.gene33775:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATCTCTAAAAATTAAAAGAA+CGG | - | chr4.3:23911301-23911320 | None:intergenic | 15.0% |
| !!! | GTAAAAAGTAATGATAATGT+AGG | - | chr4.3:23911263-23911282 | None:intergenic | 20.0% |
| ! | AAAAATTACTGGAAAGCAAT+TGG | + | chr4.3:23911383-23911402 | MS.gene33775:CDS | 25.0% |
| ! | AAAATTACTGGAAAGCAATT+GGG | + | chr4.3:23911384-23911403 | MS.gene33775:CDS | 25.0% |
| ! | TTTGTGAAAATTCTCTTGAT+TGG | - | chr4.3:23911354-23911373 | None:intergenic | 25.0% |
| AAATTACTGGAAAGCAATTG+GGG | + | chr4.3:23911385-23911404 | MS.gene33775:CDS | 30.0% | |
| TTAGAGATGAACTATGTTTG+TGG | + | chr4.3:23911313-23911332 | MS.gene33775:intron | 30.0% | |
| ! | TCAAGAGAATTTTCACAAAG+AGG | + | chr4.3:23911355-23911374 | MS.gene33775:CDS | 30.0% |
| AAAGATTATTGAGGAAGCTG+AGG | + | chr4.3:23911146-23911165 | MS.gene33775:CDS | 35.0% | |
| AAGAGGCTGACAAAAATTAC+TGG | + | chr4.3:23911372-23911391 | MS.gene33775:CDS | 35.0% | |
| ACAAGGTTCAAAACAGAGAA+AGG | + | chr4.3:23911217-23911236 | MS.gene33775:CDS | 35.0% | |
| CAAGGTTCAAAACAGAGAAA+GGG | + | chr4.3:23911218-23911237 | MS.gene33775:CDS | 35.0% | |
| GAAACAGCAACTAAACCAAA+TGG | + | chr4.3:23911629-23911648 | MS.gene33775:CDS | 35.0% | |
| GTTTGTGGAACAGTTATACT+TGG | + | chr4.3:23911328-23911347 | MS.gene33775:intron | 35.0% | |
| TGAATCTACAGTACCGTTAT+TGG | - | chr4.3:23911720-23911739 | None:intergenic | 35.0% | |
| TGAGAAGAAAAGAGGCAAAA+AGG | + | chr4.3:23911436-23911455 | MS.gene33775:CDS | 35.0% | |
| ! | AAGTGGGTTTCTATGAGAAA+AGG | + | chr4.3:23911175-23911194 | MS.gene33775:CDS | 35.0% |
| AAAGACTCCAAAGAGAAGGA+CGG | + | chr4.3:23911602-23911621 | MS.gene33775:CDS | 40.0% | |
| GAAAAGAGGCAAAAAGGATC+AGG | + | chr4.3:23911442-23911461 | MS.gene33775:CDS | 40.0% | |
| GAAGCTGAGGATTACAAAGT+GGG | + | chr4.3:23911159-23911178 | MS.gene33775:CDS | 40.0% | |
| GCCAACATTGAGAAGAAAAG+AGG | + | chr4.3:23911428-23911447 | MS.gene33775:CDS | 40.0% | |
| GCTCAATGTTGAGACAAACA+AGG | + | chr4.3:23911200-23911219 | MS.gene33775:CDS | 40.0% | |
| TAAACCAAATGGATCAGCAC+CGG | + | chr4.3:23911640-23911659 | MS.gene33775:CDS | 40.0% | |
| TCAAAACAGAGAAAGGGAGA+AGG | + | chr4.3:23911224-23911243 | MS.gene33775:CDS | 40.0% | |
| TGCTAAAGACTCCAAAGAGA+AGG | + | chr4.3:23911598-23911617 | MS.gene33775:CDS | 40.0% | |
| ! | GCCTCTTTTCTTCTCAATGT+TGG | - | chr4.3:23911432-23911451 | None:intergenic | 40.0% |
| ! | TTCTTTTCCGTCCTTCTCTT+TGG | - | chr4.3:23911612-23911631 | None:intergenic | 40.0% |
| !! | TTCTCTTTGGAGTCTTTAGC+AGG | - | chr4.3:23911599-23911618 | None:intergenic | 40.0% |
| ATATCTGCCGCATCCTAGAA+AGG | - | chr4.3:23911523-23911542 | None:intergenic | 45.0% | |
| CCAAAGGATGCTGCCAATAA+CGG | + | chr4.3:23911704-23911723 | MS.gene33775:CDS | 45.0% | |
| GCATCCTTTGGTTGTGATTC+AGG | - | chr4.3:23911695-23911714 | None:intergenic | 45.0% | |
| GGAAGCTGAGGATTACAAAG+TGG | + | chr4.3:23911158-23911177 | MS.gene33775:CDS | 45.0% | |
| GGATCAGCACCTAAACCAAA+TGG | + | chr4.3:23911650-23911669 | MS.gene33775:CDS | 45.0% | |
| TCTCAATGTTGGCAACCTCA+CGG | - | chr4.3:23911421-23911440 | None:intergenic | 45.0% | |
| TGCACCTGAATCACAACCAA+AGG | + | chr4.3:23911688-23911707 | MS.gene33775:CDS | 45.0% | |
| TGTGATTCAGGTGCAACTTC+CGG | - | chr4.3:23911683-23911702 | None:intergenic | 45.0% | |
| !! | GGTGCTGATCCATTTGGTTT+AGG | - | chr4.3:23911641-23911660 | None:intergenic | 45.0% |
| !! | GGTTTAGGTGCTGATCCATT+TGG | - | chr4.3:23911647-23911666 | None:intergenic | 45.0% |
| CCATCAGTTACTGTCCTCCA+AGG | + | chr4.3:23911473-23911492 | MS.gene33775:CDS | 50.0% | |
| CCGTTATTGGCAGCATCCTT+TGG | - | chr4.3:23911707-23911726 | None:intergenic | 50.0% | |
| CCTTGGAGGACAGTAACTGA+TGG | - | chr4.3:23911476-23911495 | None:intergenic | 50.0% | |
| CTCAATGTTGGCAACCTCAC+GGG | - | chr4.3:23911420-23911439 | None:intergenic | 50.0% | |
| GGAGGAATCATGTGAAGTGG+TGG | - | chr4.3:23911566-23911585 | None:intergenic | 50.0% | |
| GGAGGAGGAATCATGTGAAG+TGG | - | chr4.3:23911569-23911588 | None:intergenic | 50.0% | |
| TCAATGTTGGCAACCTCACG+GGG | - | chr4.3:23911419-23911438 | None:intergenic | 50.0% | |
| TTGGAGTCTTTAGCAGGTGC+AGG | - | chr4.3:23911593-23911612 | None:intergenic | 50.0% | |
| !! | ACTTCCGGTGCTGATCCATT+TGG | - | chr4.3:23911668-23911687 | None:intergenic | 50.0% |
| CGCATCCTAGAAAGGTCGGT+GGG | - | chr4.3:23911515-23911534 | None:intergenic | 55.0% | |
| CTGCCGCATCCTAGAAAGGT+CGG | - | chr4.3:23911519-23911538 | None:intergenic | 55.0% | |
| GAAAGAAGCTCCTGCCACTG+AGG | + | chr4.3:23911745-23911764 | MS.gene33775:CDS | 55.0% | |
| GAGTCTTTAGCAGGTGCAGG+TGG | - | chr4.3:23911590-23911609 | None:intergenic | 55.0% | |
| GCAAACCCACCGACCTTTCT+AGG | + | chr4.3:23911507-23911526 | MS.gene33775:CDS | 55.0% | |
| GTGGCAGGAGCTTCTTTCTG+AGG | - | chr4.3:23911743-23911762 | None:intergenic | 55.0% | |
| TCTTTAGCAGGTGCAGGTGG+AGG | - | chr4.3:23911587-23911606 | None:intergenic | 55.0% | |
| TGGGGAGATAATTCCCCGTG+AGG | + | chr4.3:23911403-23911422 | MS.gene33775:CDS | 55.0% | |
| !! | CCACCGACCTTTCTAGGATG+CGG | + | chr4.3:23911513-23911532 | MS.gene33775:CDS | 55.0% |
| CCGCATCCTAGAAAGGTCGG+TGG | - | chr4.3:23911516-23911535 | None:intergenic | 60.0% | |
| TGCAGCAGACTGCTCCTCAG+TGG | - | chr4.3:23911762-23911781 | None:intergenic | 60.0% | |
| TTAGCAGGTGCAGGTGGAGG+AGG | - | chr4.3:23911584-23911603 | None:intergenic | 60.0% | |
| ! | CGGTGGGTTTGCCTGGCTTT+GGG | - | chr4.3:23911499-23911518 | None:intergenic | 60.0% |
| ! | GAAAGGTCGGTGGGTTTGCC+TGG | - | chr4.3:23911506-23911525 | None:intergenic | 60.0% |
| ! | TCGGTGGGTTTGCCTGGCTT+TGG | - | chr4.3:23911500-23911519 | None:intergenic | 60.0% |
| !! | GTTTGCCTGGCTTTGGGCCT+TGG | - | chr4.3:23911493-23911512 | None:intergenic | 60.0% |
| GCAGACTGCTCCTCAGTGGC+AGG | - | chr4.3:23911758-23911777 | None:intergenic | 65.0% | |
| GTCCTCCAAGGCCCAAAGCC+AGG | + | chr4.3:23911485-23911504 | MS.gene33775:CDS | 65.0% | |
| !! | TGCCTGGCTTTGGGCCTTGG+AGG | - | chr4.3:23911490-23911509 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 23911138 | 23911784 | 23911138 | ID=MS.gene33775 |
| chr4.3 | mRNA | 23911138 | 23911784 | 23911138 | ID=MS.gene33775.t1;Parent=MS.gene33775 |
| chr4.3 | exon | 23911138 | 23911245 | 23911138 | ID=MS.gene33775.t1.exon1;Parent=MS.gene33775.t1 |
| chr4.3 | CDS | 23911138 | 23911245 | 23911138 | ID=cds.MS.gene33775.t1;Parent=MS.gene33775.t1 |
| chr4.3 | exon | 23911341 | 23911784 | 23911341 | ID=MS.gene33775.t1.exon2;Parent=MS.gene33775.t1 |
| chr4.3 | CDS | 23911341 | 23911784 | 23911341 | ID=cds.MS.gene33775.t1;Parent=MS.gene33775.t1 |
| Gene Sequence |
| Protein sequence |
