AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene34148

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34148.t1 MTR_1g100950 96.861 446 6 1 1 438 41 486 0.0 897
MS.gene34148.t1 MTR_1g100950 96.861 446 6 1 1 438 1 446 0.0 896
MS.gene34148.t1 MTR_1g100950 95.389 347 8 1 1 339 1 347 0.0 686
MS.gene34148.t1 MTR_8g030890 66.815 449 134 6 1 438 1 445 0.0 606
MS.gene34148.t1 MTR_3g065480 57.111 443 174 5 1 430 1 440 0.0 521
MS.gene34148.t1 MTR_1g100950 91.935 248 16 1 1 248 1 244 4.89e-169 474
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34148.t1 AT3G49050 70.339 472 106 5 1 438 1 472 0.0 674
MS.gene34148.t1 AT4G00500 62.138 449 156 5 1 437 1 447 0.0 558
MS.gene34148.t1 AT4G00500 62.138 449 156 5 1 437 1 447 0.0 558
MS.gene34148.t1 AT5G37710 56.376 447 170 6 1 437 1 432 2.12e-176 500
MS.gene34148.t1 AT5G37710 64.183 349 112 4 1 339 1 346 1.76e-161 459

Find 116 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCTGTTGGATAATAAATT+AGG 0.146068 1.2:-75189246 MS.gene34148:CDS
CGTCAGATCATGCCATTATT+TGG 0.173210 1.2:-75187804 MS.gene34148:CDS
CGCTGCATGATTTGCTTTAC+AGG 0.173921 1.2:+75186739 None:intergenic
GTAATGCAGCTTTGTATTCT+TGG 0.174372 1.2:+75186692 None:intergenic
TTGTTGAGAGAAAGCCTTTC+AGG 0.219282 1.2:-75188109 MS.gene34148:intron
AATTATACTCTTACATTTGC+TGG 0.240349 1.2:-75189114 MS.gene34148:CDS
CTGATATAGTTCTTGCTATA+AGG 0.241938 1.2:-75189302 MS.gene34148:CDS
TAGATGTATGTCGCTTAATT+TGG 0.247337 1.2:-75188977 MS.gene34148:CDS
ACGATCTTCGGAACCCTCTT+TGG 0.259036 1.2:-75189437 MS.gene34148:CDS
GCAAATGTAAGAGTATAATT+TGG 0.291177 1.2:+75189117 None:intergenic
TGTTCACAATGGGCTGTTAA+AGG 0.304854 1.2:-75189199 MS.gene34148:CDS
CTTCTGTTAGTTCAACTAAT+AGG 0.307521 1.2:-75186567 MS.gene34148:CDS
TTTACGCCACCATCAGAATA+TGG 0.317987 1.2:-75186637 MS.gene34148:CDS
TGCTATAAGGGGACTAAATC+TGG 0.319987 1.2:-75189289 MS.gene34148:CDS
TTTCTAAGTAATAACCAATC+AGG 0.324026 1.2:+75189387 None:intergenic
ATTGTTTAGCTTGCGCTCGT+TGG 0.340664 1.2:-75189578 MS.gene34148:CDS
CTCTTTCTCTATCCAAATAA+TGG 0.346939 1.2:+75187792 None:intergenic
AACCCTCTTTGGGCGCCTCC+TGG 0.352249 1.2:-75189426 MS.gene34148:CDS
TGATCAAGATACAATATATA+TGG 0.354509 1.2:+75189333 None:intergenic
TGCCATGCTTATTGTGCTTA+AGG 0.358859 1.2:-75188214 MS.gene34148:CDS
CGATCTTCGGAACCCTCTTT+GGG 0.364296 1.2:-75189436 MS.gene34148:CDS
TTCTGTTGGATAATAAATTA+GGG 0.368729 1.2:-75189245 MS.gene34148:CDS
ATATTGAGAGGAAGAGAGTT+AGG 0.369624 1.2:-75189020 MS.gene34148:CDS
GGCACTGCTGTTCTCACAAC+TGG 0.373000 1.2:+75187871 None:intergenic
ACTTACATCCAAAGCCCTTT+GGG 0.382582 1.2:+75187768 None:intergenic
GACTTACATCCAAAGCCCTT+TGG 0.385089 1.2:+75187767 None:intergenic
TGATATAGTTCTTGCTATAA+GGG 0.394351 1.2:-75189301 MS.gene34148:CDS
TTGTTTAGCTTGCGCTCGTT+GGG 0.398289 1.2:-75189577 MS.gene34148:CDS
GAAAGAGGCCCAAAGGGCTT+TGG 0.400956 1.2:-75187776 MS.gene34148:CDS
ACATTTGCTGGACATTCCCT+TGG 0.407940 1.2:-75189102 MS.gene34148:CDS
AGAACAGCAGTGCCAGTAGA+TGG 0.408642 1.2:-75187862 MS.gene34148:CDS
CAGGGCATGACAGTGAAACT+TGG 0.415218 1.2:-75189530 MS.gene34148:CDS
GCTGGACATTCCCTTGGATC+AGG 0.417188 1.2:-75189096 MS.gene34148:CDS
AGGAAGGAGAGGAGAGTTCA+AGG 0.427280 1.2:-75186603 MS.gene34148:CDS
AAAGGCTGCTGGATGGGTTA+TGG 0.430445 1.2:-75189181 MS.gene34148:CDS
CTGTGTATGAAGACGATCTT+CGG 0.437451 1.2:-75189449 MS.gene34148:CDS
ACTTGAATATATCTTCCAAC+GGG 0.444494 1.2:+75188415 None:intergenic
TCCAACGGGGTGGCTGTCCT+TGG 0.464056 1.2:+75188429 None:intergenic
GGAGTACGGTTAACGAAAGC+TGG 0.464646 1.2:-75186546 MS.gene34148:CDS
TAGCTTGCGCTCGTTGGGCG+TGG 0.470914 1.2:-75189572 MS.gene34148:CDS
TGGGCGCCTCCTGGTGGCTA+TGG 0.472533 1.2:-75189417 MS.gene34148:CDS
AAAGCAAATCATGCAGCGTC+AGG 0.480132 1.2:-75186734 MS.gene34148:CDS
AATGGGCTGTTAAAGGCTGC+TGG 0.482503 1.2:-75189192 MS.gene34148:CDS
TGCTCGAACCTTCCATCTAC+TGG 0.484062 1.2:+75187850 None:intergenic
CCAGGTGCATAGAGTCTCCT+TGG 0.485341 1.2:+75188146 None:intergenic
CACCAGGAGGCGCCCAAAGA+GGG 0.495968 1.2:+75189424 None:intergenic
CTGCCAATTGCAGTTGATGA+TGG 0.503555 1.2:-75186785 MS.gene34148:intron
TGCTGGATGGGTTATGGATG+CGG 0.509716 1.2:-75189175 MS.gene34148:CDS
GCTATGGAATCAACCCTGAT+TGG 0.521714 1.2:-75189401 MS.gene34148:CDS
AAAAGATGTCTCCACACTGC+AGG 0.522926 1.2:-75189549 MS.gene34148:CDS
CATTATTTGGATAGAGAAAG+AGG 0.525363 1.2:-75187791 MS.gene34148:CDS
GCTGTTAAAGGCTGCTGGAT+GGG 0.527172 1.2:-75189187 MS.gene34148:CDS
GATAGAGAAAGAGGCCCAAA+GGG 0.528361 1.2:-75187782 MS.gene34148:CDS
TTAGGGAAGAGAAAGTTTGA+TGG 0.529142 1.2:-75189228 MS.gene34148:CDS
AGGGCATGACAGTGAAACTT+GGG 0.536314 1.2:-75189529 MS.gene34148:CDS
AGGCTCAAATTCTTCTGTTG+CGG 0.536990 1.2:+75189500 None:intergenic
GGCTGTTAAAGGCTGCTGGA+TGG 0.539589 1.2:-75189188 MS.gene34148:CDS
CCACCAGGAGGCGCCCAAAG+AGG 0.544467 1.2:+75189423 None:intergenic
TAGCGACAAAGACGTGGGAC+AGG 0.547885 1.2:+75189480 None:intergenic
TTGCTTTACAGGTACTTCCA+TGG 0.554356 1.2:+75186750 None:intergenic
CCTCTTTGGGCGCCTCCTGG+TGG 0.558537 1.2:-75189423 MS.gene34148:CDS
TAGGGAAGAGAAAGTTTGAT+GGG 0.559444 1.2:-75189227 MS.gene34148:CDS
CTGACGTAGCATTACAAGAA+AGG 0.560213 1.2:+75187821 None:intergenic
CCAAGGAGACTCTATGCACC+TGG 0.561142 1.2:-75188146 MS.gene34148:CDS
GAGTGATTATGCTGTTCTGT+TGG 0.561183 1.2:-75189259 MS.gene34148:CDS
TTCTAAGTAATAACCAATCA+GGG 0.562621 1.2:+75189388 None:intergenic
CTGCAGCTACTCCTGATCCA+AGG 0.564734 1.2:+75189085 None:intergenic
TCAAAAGTTCCATATTCTGA+TGG 0.568938 1.2:+75186628 None:intergenic
GGGAAGAGAAAGTTTGATGG+GGG 0.571897 1.2:-75189225 MS.gene34148:CDS
AATCCTTAGCCATACCTGAA+AGG 0.572964 1.2:+75188095 None:intergenic
TTAAGCGACATACATCTAGC+AGG 0.579156 1.2:+75188982 None:intergenic
TAGTTCAACTAATAGGAGTA+CGG 0.579759 1.2:-75186560 MS.gene34148:CDS
AGAGGAGAGTTCAAGGTCAG+AGG 0.582485 1.2:-75186596 MS.gene34148:CDS
GACTTGAATATATCTTCCAA+CGG 0.583155 1.2:+75188414 None:intergenic
TGCAGCTACTCCTGATCCAA+GGG 0.585146 1.2:+75189086 None:intergenic
AGTAGCTGCAGCGTTGAGCA+TGG 0.585878 1.2:-75189073 MS.gene34148:CDS
AGAAGATGCTAAAAGATCCA+AGG 0.590230 1.2:-75188163 MS.gene34148:CDS
CAATATACACACTCAACAAG+AGG 0.590440 1.2:+75189597 None:intergenic
CACCTTAAGCACAATAAGCA+TGG 0.593008 1.2:+75188212 None:intergenic
GGATAGAGAAAGAGGCCCAA+AGG 0.594158 1.2:-75187783 MS.gene34148:CDS
AAAGATGTCTCCACACTGCA+GGG 0.595097 1.2:-75189548 MS.gene34148:CDS
CAGCAGTGCCAGTAGATGGA+AGG 0.597618 1.2:-75187858 MS.gene34148:CDS
CAGGAAACAATGACAAGACA+CGG 0.602595 1.2:-75186715 MS.gene34148:CDS
TCAAGATACAATATATATGG+TGG 0.604903 1.2:+75189336 None:intergenic
AAAATATAGCGACAAAGACG+TGG 0.605455 1.2:+75189474 None:intergenic
GTACAAAGATACAAGAGGAC+GGG 0.607406 1.2:-75189361 MS.gene34148:CDS
TGTTTCTGTGCAGATTGGGA+AGG 0.608268 1.2:-75187900 MS.gene34148:intron
ACCAAGGACAGCCACCCCGT+TGG 0.612019 1.2:-75188430 MS.gene34148:CDS
GAGTACGGTTAACGAAAGCT+GGG 0.612808 1.2:-75186545 MS.gene34148:CDS
AAGGGGACTAAATCTGGCAA+AGG 0.614888 1.2:-75189283 MS.gene34148:CDS
GTGCAGAATCGAGATAGACT+TGG 0.616158 1.2:-75189045 MS.gene34148:CDS
ACTGCTGTTCTCACAACTGG+AGG 0.621198 1.2:+75187874 None:intergenic
AAGACGTACAAAGATACAAG+AGG 0.622138 1.2:-75189366 MS.gene34148:CDS
GATATAGTTCTTGCTATAAG+GGG 0.623233 1.2:-75189300 MS.gene34148:CDS
GGGTTGATTCCATAGCCACC+AGG 0.625363 1.2:+75189408 None:intergenic
GTCACAATGTCGATTATCTG+CGG 0.627450 1.2:-75189624 None:intergenic
CACTGTCATGCCCTGCAGTG+TGG 0.628154 1.2:+75189538 None:intergenic
GATGGAGAAAGATAATACCA+TGG 0.629976 1.2:-75186767 MS.gene34148:CDS
GTTAGGATGACTTCTTACCA+AGG 0.630330 1.2:-75188446 MS.gene34148:intron
TATTGTGCTTAAGGTGCATG+AGG 0.631803 1.2:-75188205 MS.gene34148:CDS
AAAGTTCCATATTCTGATGG+TGG 0.632248 1.2:+75186631 None:intergenic
ATAGACTTGGAAATATTGAG+AGG 0.643093 1.2:-75189032 MS.gene34148:CDS
AGGGAAGAGAAAGTTTGATG+GGG 0.643212 1.2:-75189226 MS.gene34148:CDS
ATTGTGCTTAAGGTGCATGA+GGG 0.654264 1.2:-75188204 MS.gene34148:CDS
ACAATGTGATACAAGCGACC+AGG 0.658544 1.2:+75188128 None:intergenic
GGAAGAGAAAGTTTGATGGG+GGG 0.658997 1.2:-75189224 MS.gene34148:CDS
GAATATATCTTCCAACGGGG+TGG 0.663765 1.2:+75188419 None:intergenic
CGTACAAAGATACAAGAGGA+CGG 0.663943 1.2:-75189362 MS.gene34148:CDS
TAAGGATGAGCATGGTCACA+TGG 0.671907 1.2:-75186497 MS.gene34148:CDS
GATGGTGGCGTAAAAGCATG+TGG 0.680362 1.2:+75186646 None:intergenic
AAATATAGCGACAAAGACGT+GGG 0.685383 1.2:+75189475 None:intergenic
GAGGGATACATGTATACCAG+AGG 0.695751 1.2:-75188186 MS.gene34148:CDS
GGGCATGACAGTGAAACTTG+GGG 0.699008 1.2:-75189528 MS.gene34148:CDS
AGCTGCAGCGTTGAGCATGG+TGG 0.702176 1.2:-75189070 MS.gene34148:CDS
TTGATTCCATAGCCACCAGG+AGG 0.734365 1.2:+75189411 None:intergenic
CTTGAATATATCTTCCAACG+GGG 0.749285 1.2:+75188416 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGAAGTACAAAAATTACA+AGG + chr1.2:75188366-75188385 None:intergenic 20.0%
!! CTAGATGTAAAAATATTCAA+AGG + chr1.2:75187238-75187257 None:intergenic 20.0%
!! TATCACATAAAATAAACTTC+AGG + chr1.2:75187385-75187404 None:intergenic 20.0%
!! TCTAAGAATTTCAAATCTTA+AGG + chr1.2:75188462-75188481 None:intergenic 20.0%
!! TGATCAAGATACAATATATA+TGG + chr1.2:75186771-75186790 None:intergenic 20.0%
!! TGTCAACTATAATTAAGATT+TGG - chr1.2:75188436-75188455 MS.gene34148:CDS 20.0%
!! TTCTGTTGGATAATAAATTA+GGG - chr1.2:75186856-75186875 MS.gene34148:intron 20.0%
!! TTTATTATGAGAGATAGTAA+AGG - chr1.2:75187439-75187458 MS.gene34148:intron 20.0%
!!! AATTTTTGTACTTCATTCTT+TGG - chr1.2:75188369-75188388 MS.gene34148:intron 20.0%
!!! TATGTTTTCGTAAAATATCA+TGG + chr1.2:75188801-75188820 None:intergenic 20.0%
! AATTATACTCTTACATTTGC+TGG - chr1.2:75186987-75187006 MS.gene34148:intron 25.0%
! ATAAGCTCCAATAAGTAATA+AGG + chr1.2:75188686-75188705 None:intergenic 25.0%
! ATGAAGCTGAAAACAATTTA+TGG + chr1.2:75188771-75188790 None:intergenic 25.0%
! GTATGCATACAAATTATCAT+AGG - chr1.2:75188629-75188648 MS.gene34148:intron 25.0%
! GTTCTGTTGGATAATAAATT+AGG - chr1.2:75186855-75186874 MS.gene34148:intron 25.0%
! TAAATAGAATGCCAAATTAG+TGG + chr1.2:75189259-75189278 None:intergenic 25.0%
! TCAAGATACAATATATATGG+TGG + chr1.2:75186768-75186787 None:intergenic 25.0%
! TGAAGCTGAAAACAATTTAT+GGG + chr1.2:75188770-75188789 None:intergenic 25.0%
! TGGAACTTGATTGATTATTT+AGG + chr1.2:75189239-75189258 None:intergenic 25.0%
! TTAGTTAATTTCAACTGTTG+AGG - chr1.2:75187529-75187548 MS.gene34148:intron 25.0%
! TTCTAAGTAATAACCAATCA+GGG + chr1.2:75186716-75186735 None:intergenic 25.0%
! TTTCTAAGTAATAACCAATC+AGG + chr1.2:75186717-75186736 None:intergenic 25.0%
!! ATATATTCAAGTCTCTTTTC+TGG - chr1.2:75187697-75187716 MS.gene34148:intron 25.0%
!! ATGAGAAGATATTGTTTTCT+TGG - chr1.2:75187854-75187873 MS.gene34148:CDS 25.0%
!! GCAAATGTAAGAGTATAATT+TGG + chr1.2:75186987-75187006 None:intergenic 25.0%
!! TAATTTGTATGCATACTTGA+AGG + chr1.2:75188625-75188644 None:intergenic 25.0%
!! TGATATAGTTCTTGCTATAA+GGG - chr1.2:75186800-75186819 MS.gene34148:intron 25.0%
AAATATTCAAAGGAACGACA+AGG + chr1.2:75187228-75187247 None:intergenic 30.0%
AAATGTAAAGCCAAACGAAA+AGG + chr1.2:75188173-75188192 None:intergenic 30.0%
ACTTGAATATATCTTCCAAC+GGG + chr1.2:75187689-75187708 None:intergenic 30.0%
AGTAATAAGGCAATCCAAAT+AGG + chr1.2:75188673-75188692 None:intergenic 30.0%
ATAACGAAGTTAGTCGTATT+AGG + chr1.2:75187737-75187756 None:intergenic 30.0%
ATAGACTTGGAAATATTGAG+AGG - chr1.2:75187069-75187088 MS.gene34148:intron 30.0%
ATGCATACTTGAAGGAAATA+TGG + chr1.2:75188617-75188636 None:intergenic 30.0%
CATTATTTGGATAGAGAAAG+AGG - chr1.2:75188310-75188329 MS.gene34148:intron 30.0%
CTCTTTCTCTATCCAAATAA+TGG + chr1.2:75188312-75188331 None:intergenic 30.0%
CTTCTGTTAGTTCAACTAAT+AGG - chr1.2:75189534-75189553 MS.gene34148:CDS 30.0%
GAAGGCAATTTCCAAAATTA+TGG + chr1.2:75188059-75188078 None:intergenic 30.0%
GACTTGAATATATCTTCCAA+CGG + chr1.2:75187690-75187709 None:intergenic 30.0%
GTCAGCTTGAATAAGTATAA+GGG + chr1.2:75187586-75187605 None:intergenic 30.0%
TAACGAAGTTAGTCGTATTA+GGG + chr1.2:75187736-75187755 None:intergenic 30.0%
TAGTTCAACTAATAGGAGTA+CGG - chr1.2:75189541-75189560 MS.gene34148:CDS 30.0%
TATCAATTTGAGCTTCTTGT+AGG - chr1.2:75189213-75189232 MS.gene34148:CDS 30.0%
TCAAAAGTTCCATATTCTGA+TGG + chr1.2:75189476-75189495 None:intergenic 30.0%
TCAATCAAGTTCCACTAATT+TGG - chr1.2:75189245-75189264 MS.gene34148:CDS 30.0%
! AGAATATGGAACTTTTGATG+AGG - chr1.2:75189478-75189497 MS.gene34148:CDS 30.0%
! CAGAACTGTAACCATAATTT+TGG - chr1.2:75188045-75188064 MS.gene34148:intron 30.0%
! CTGATATAGTTCTTGCTATA+AGG - chr1.2:75186799-75186818 MS.gene34148:intron 30.0%
! GATATAGTTCTTGCTATAAG+GGG - chr1.2:75186801-75186820 MS.gene34148:intron 30.0%
! TAGATGTATGTCGCTTAATT+TGG - chr1.2:75187124-75187143 MS.gene34148:intron 30.0%
! TGGATTGCCTTATTACTTAT+TGG - chr1.2:75188676-75188695 MS.gene34148:intron 30.0%
! TGTTAAATTAGAGCTTTTGC+TGG - chr1.2:75187272-75187291 MS.gene34148:intron 30.0%
! TTTTGTACTTCATTCTTTGG+TGG - chr1.2:75188372-75188391 MS.gene34148:intron 30.0%
!! AAGTTAATCGCTATTTTCCT+TGG - chr1.2:75187633-75187652 MS.gene34148:intron 30.0%
AAAATATAGCGACAAAGACG+TGG + chr1.2:75186630-75186649 None:intergenic 35.0%
AAAGTTCCATATTCTGATGG+TGG + chr1.2:75189473-75189492 None:intergenic 35.0%
AAATATAGCGACAAAGACGT+GGG + chr1.2:75186629-75186648 None:intergenic 35.0%
AAGACGTACAAAGATACAAG+AGG - chr1.2:75186735-75186754 MS.gene34148:CDS 35.0%
AATCCAAATAGGCCATAAGT+AGG + chr1.2:75188662-75188681 None:intergenic 35.0%
ACTTATTGGAGCTTATCTAC+TGG - chr1.2:75188690-75188709 MS.gene34148:intron 35.0%
AGAAGATGCTAAAAGATCCA+AGG - chr1.2:75187938-75187957 MS.gene34148:intron 35.0%
ATACACTGTGTTTGAACTGA+AGG + chr1.2:75188077-75188096 None:intergenic 35.0%
ATATTGAGAGGAAGAGAGTT+AGG - chr1.2:75187081-75187100 MS.gene34148:intron 35.0%
ATCCAAATAGGCCATAAGTA+GGG + chr1.2:75188661-75188680 None:intergenic 35.0%
ATGCATACTTGAAGGACATA+TGG + chr1.2:75188915-75188934 None:intergenic 35.0%
CAATATACACACTCAACAAG+AGG + chr1.2:75186507-75186526 None:intergenic 35.0%
CAGAGAGAAACAATTTATGG+CGG - chr1.2:75187318-75187337 MS.gene34148:intron 35.0%
CTTGAATATATCTTCCAACG+GGG + chr1.2:75187688-75187707 None:intergenic 35.0%
GATGGAGAAAGATAATACCA+TGG - chr1.2:75189334-75189353 MS.gene34148:CDS 35.0%
GGTCAGCTTGAATAAGTATA+AGG + chr1.2:75187587-75187606 None:intergenic 35.0%
TACAAGATTCAGCATGAATC+AGG + chr1.2:75187608-75187627 None:intergenic 35.0%
TAGGGAAGAGAAAGTTTGAT+GGG - chr1.2:75186874-75186893 MS.gene34148:intron 35.0%
TGCCAGAGAGAAACAATTTA+TGG - chr1.2:75187315-75187334 MS.gene34148:intron 35.0%
TTAGGGAAGAGAAAGTTTGA+TGG - chr1.2:75186873-75186892 MS.gene34148:intron 35.0%
TTTCAACTGTTGAGGTTAAG+TGG - chr1.2:75187537-75187556 MS.gene34148:intron 35.0%
! CAATCGAACATTTTTCCATC+AGG + chr1.2:75187412-75187431 None:intergenic 35.0%
! CTTTTTGATAAGGATGAGCA+TGG - chr1.2:75189596-75189615 MS.gene34148:CDS 35.0%
! GATTGAGCGTCTTTTTGATA+AGG - chr1.2:75189586-75189605 MS.gene34148:CDS 35.0%
! GTAATGCAGCTTTGTATTCT+TGG + chr1.2:75189412-75189431 None:intergenic 35.0%
! TATGGAACTTTTGATGAGGA+AGG - chr1.2:75189482-75189501 MS.gene34148:CDS 35.0%
!! AATCGCTATTTTCCTTGGTT+AGG - chr1.2:75187638-75187657 MS.gene34148:intron 35.0%
!! GTGTGAAATTTTGAGGGAAT+TGG - chr1.2:75186950-75186969 MS.gene34148:intron 35.0%
!!! CTTTTTGATTTCCCTACACA+TGG + chr1.2:75188576-75188595 None:intergenic 35.0%
AATCCTTAGCCATACCTGAA+AGG + chr1.2:75188009-75188028 None:intergenic 40.0%
ACTTACATCCAAAGCCCTTT+GGG + chr1.2:75188336-75188355 None:intergenic 40.0%
AGGCTCAAATTCTTCTGTTG+CGG + chr1.2:75186604-75186623 None:intergenic 40.0%
AGGGAAGAGAAAGTTTGATG+GGG - chr1.2:75186875-75186894 MS.gene34148:intron 40.0%
ATTGTGCTTAAGGTGCATGA+GGG - chr1.2:75187897-75187916 MS.gene34148:intron 40.0%
CACCTTAAGCACAATAAGCA+TGG + chr1.2:75187892-75187911 None:intergenic 40.0%
CAGGAAACAATGACAAGACA+CGG - chr1.2:75189386-75189405 MS.gene34148:CDS 40.0%
CGTACAAAGATACAAGAGGA+CGG - chr1.2:75186739-75186758 MS.gene34148:CDS 40.0%
CGTCAGATCATGCCATTATT+TGG - chr1.2:75188297-75188316 MS.gene34148:intron 40.0%
CTGACGTAGCATTACAAGAA+AGG + chr1.2:75188283-75188302 None:intergenic 40.0%
CTGTGTATGAAGACGATCTT+CGG - chr1.2:75186652-75186671 MS.gene34148:CDS 40.0%
GAGTGATTATGCTGTTCTGT+TGG - chr1.2:75186842-75186861 MS.gene34148:intron 40.0%
GTAAGAAGTCATCCTAACCA+AGG + chr1.2:75187653-75187672 None:intergenic 40.0%
GTACAAAGATACAAGAGGAC+GGG - chr1.2:75186740-75186759 MS.gene34148:CDS 40.0%
GTTAGGATGACTTCTTACCA+AGG - chr1.2:75187655-75187674 MS.gene34148:intron 40.0%
TACCACATCTACCATGTGTA+GGG - chr1.2:75188562-75188581 MS.gene34148:intron 40.0%
TATTGTGCTTAAGGTGCATG+AGG - chr1.2:75187896-75187915 MS.gene34148:intron 40.0%
TCCAAATAGGCCATAAGTAG+GGG + chr1.2:75188660-75188679 None:intergenic 40.0%
TCTCCATCATCAACTGCAAT+TGG + chr1.2:75189322-75189341 None:intergenic 40.0%
TGCCATGCTTATTGTGCTTA+AGG - chr1.2:75187887-75187906 MS.gene34148:CDS 40.0%
TGCTATAAGGGGACTAAATC+TGG - chr1.2:75186812-75186831 MS.gene34148:intron 40.0%
TTAAGCGACATACATCTAGC+AGG + chr1.2:75187122-75187141 None:intergenic 40.0%
TTGCTTTACAGGTACTTCCA+TGG + chr1.2:75189354-75189373 None:intergenic 40.0%
TTGTTGAGAGAAAGCCTTTC+AGG - chr1.2:75187992-75188011 MS.gene34148:intron 40.0%
TTTACGCCACCATCAGAATA+TGG - chr1.2:75189464-75189483 MS.gene34148:CDS 40.0%
! AACTTTTGATGAGGAAGGAG+AGG - chr1.2:75189487-75189506 MS.gene34148:CDS 40.0%
! ATAGGTTTTCCCCCTACTTA+TGG - chr1.2:75188647-75188666 MS.gene34148:intron 40.0%
! ATGCGGAGTGTGAAATTTTG+AGG - chr1.2:75186943-75186962 MS.gene34148:intron 40.0%
! CGCCATAAATTGTTTCTCTC+TGG + chr1.2:75187320-75187339 None:intergenic 40.0%
! GCTTATACAGCCTTTTCGTT+TGG - chr1.2:75188160-75188179 MS.gene34148:CDS 40.0%
! TCTTTTAGCATCTTCTCCTC+TGG + chr1.2:75187934-75187953 None:intergenic 40.0%
! TGCGGAGTGTGAAATTTTGA+GGG - chr1.2:75186944-75186963 MS.gene34148:intron 40.0%
! TTTATGTGATAGCGACCTGA+TGG - chr1.2:75187394-75187413 MS.gene34148:intron 40.0%
!! AACACTGTTTCTGTGCAGAT+TGG - chr1.2:75188196-75188215 MS.gene34148:CDS 40.0%
!! ACACTGTTTCTGTGCAGATT+GGG - chr1.2:75188197-75188216 MS.gene34148:CDS 40.0%
!! TGTTCACAATGGGCTGTTAA+AGG - chr1.2:75186902-75186921 MS.gene34148:intron 40.0%
AAAAGATGTCTCCACACTGC+AGG - chr1.2:75186552-75186571 MS.gene34148:CDS 45.0%
AAAGATGTCTCCACACTGCA+GGG - chr1.2:75186553-75186572 MS.gene34148:CDS 45.0%
AAAGCAAATCATGCAGCGTC+AGG - chr1.2:75189367-75189386 MS.gene34148:CDS 45.0%
AAGGGGACTAAATCTGGCAA+AGG - chr1.2:75186818-75186837 MS.gene34148:intron 45.0%
ACAATGTGATACAAGCGACC+AGG + chr1.2:75187976-75187995 None:intergenic 45.0%
AGGGCATGACAGTGAAACTT+GGG - chr1.2:75186572-75186591 MS.gene34148:CDS 45.0%
CATAAACTCTGTCGTGCTTC+AGG - chr1.2:75187166-75187185 MS.gene34148:intron 45.0%
CCAAATAGGCCATAAGTAGG+GGG + chr1.2:75188659-75188678 None:intergenic 45.0%
CTACCACATCTACCATGTGT+AGG - chr1.2:75188561-75188580 MS.gene34148:intron 45.0%
GAATATATCTTCCAACGGGG+TGG + chr1.2:75187685-75187704 None:intergenic 45.0%
GACTTACATCCAAAGCCCTT+TGG + chr1.2:75188337-75188356 None:intergenic 45.0%
GAGGGATACATGTATACCAG+AGG - chr1.2:75187915-75187934 MS.gene34148:intron 45.0%
GAGTACGGTTAACGAAAGCT+GGG - chr1.2:75189556-75189575 MS.gene34148:CDS 45.0%
GATAGAGAAAGAGGCCCAAA+GGG - chr1.2:75188319-75188338 MS.gene34148:intron 45.0%
GCTATGGAATCAACCCTGAT+TGG - chr1.2:75186700-75186719 MS.gene34148:CDS 45.0%
GGAAGAGAAAGTTTGATGGG+GGG - chr1.2:75186877-75186896 MS.gene34148:intron 45.0%
GGGAAGAGAAAGTTTGATGG+GGG - chr1.2:75186876-75186895 MS.gene34148:intron 45.0%
GTGCAGAATCGAGATAGACT+TGG - chr1.2:75187056-75187075 MS.gene34148:intron 45.0%
TAAGGATGAGCATGGTCACA+TGG - chr1.2:75189604-75189623 MS.gene34148:CDS 45.0%
TCATCAACTGCAATTGGCAG+TGG + chr1.2:75189316-75189335 None:intergenic 45.0%
TGAGCACTTGTGAGACTAGT+TGG - chr1.2:75188715-75188734 MS.gene34148:intron 45.0%
TGGTAGATGTGGTAGACACA+TGG + chr1.2:75188556-75188575 None:intergenic 45.0%
TTCCCTACACATGGTAGATG+TGG + chr1.2:75188567-75188586 None:intergenic 45.0%
! AAGCCTTTCAGGTATGGCTA+AGG - chr1.2:75188003-75188022 MS.gene34148:intron 45.0%
! ACATTTGCTGGACATTCCCT+TGG - chr1.2:75186999-75187018 MS.gene34148:intron 45.0%
! AGCACTTGTGAGACTAGTTG+GGG - chr1.2:75188717-75188736 MS.gene34148:intron 45.0%
! ATGGGGGGTATGTTCACAAT+GGG - chr1.2:75186892-75186911 MS.gene34148:intron 45.0%
! GAGAGAAAGCCTTTCAGGTA+TGG - chr1.2:75187997-75188016 MS.gene34148:intron 45.0%
! GAGCACTTGTGAGACTAGTT+GGG - chr1.2:75188716-75188735 MS.gene34148:intron 45.0%
! TGTTTCTGTGCAGATTGGGA+AGG - chr1.2:75188201-75188220 MS.gene34148:CDS 45.0%
!! ATTGTTTAGCTTGCGCTCGT+TGG - chr1.2:75186523-75186542 MS.gene34148:CDS 45.0%
!! CGCTGCATGATTTGCTTTAC+AGG + chr1.2:75189365-75189384 None:intergenic 45.0%
!! CTGCCAATTGCAGTTGATGA+TGG - chr1.2:75189316-75189335 MS.gene34148:CDS 45.0%
!! TTGTTTAGCTTGCGCTCGTT+GGG - chr1.2:75186524-75186543 MS.gene34148:CDS 45.0%
!!! TTTTTTTTATTATATATCAA+TGG + chr1.2:75188137-75188156 None:intergenic 5.0%
ACGATCTTCGGAACCCTCTT+TGG - chr1.2:75186664-75186683 MS.gene34148:CDS 50.0%
ACTGCTGTTCTCACAACTGG+AGG + chr1.2:75188230-75188249 None:intergenic 50.0%
AGAACAGCAGTGCCAGTAGA+TGG - chr1.2:75188239-75188258 MS.gene34148:intron 50.0%
AGAGGAGAGTTCAAGGTCAG+AGG - chr1.2:75189505-75189524 MS.gene34148:CDS 50.0%
AGGAAGGAGAGGAGAGTTCA+AGG - chr1.2:75189498-75189517 MS.gene34148:CDS 50.0%
CAGGGCATGACAGTGAAACT+TGG - chr1.2:75186571-75186590 MS.gene34148:CDS 50.0%
CCCCCTACTTATGGCCTATT+TGG - chr1.2:75188656-75188675 MS.gene34148:intron 50.0%
CGATCTTCGGAACCCTCTTT+GGG - chr1.2:75186665-75186684 MS.gene34148:CDS 50.0%
GCTGTTAAAGGCTGCTGGAT+GGG - chr1.2:75186914-75186933 MS.gene34148:intron 50.0%
GGAGTACGGTTAACGAAAGC+TGG - chr1.2:75189555-75189574 MS.gene34148:CDS 50.0%
GGATAGAGAAAGAGGCCCAA+AGG - chr1.2:75188318-75188337 MS.gene34148:intron 50.0%
GGGCATGACAGTGAAACTTG+GGG - chr1.2:75186573-75186592 MS.gene34148:CDS 50.0%
TGCAGCTACTCCTGATCCAA+GGG + chr1.2:75187018-75187037 None:intergenic 50.0%
TGCTCGAACCTTCCATCTAC+TGG + chr1.2:75188254-75188273 None:intergenic 50.0%
TTGATTCCATAGCCACCAGG+AGG + chr1.2:75186693-75186712 None:intergenic 50.0%
! GATGGGGGGTATGTTCACAA+TGG - chr1.2:75186891-75186910 MS.gene34148:intron 50.0%
! GATGGTGGCGTAAAAGCATG+TGG + chr1.2:75189458-75189477 None:intergenic 50.0%
! GCACTTGTGAGACTAGTTGG+GGG - chr1.2:75188718-75188737 MS.gene34148:intron 50.0%
!! AAAGGCTGCTGGATGGGTTA+TGG - chr1.2:75186920-75186939 MS.gene34148:intron 50.0%
!! AATGGGCTGTTAAAGGCTGC+TGG - chr1.2:75186909-75186928 MS.gene34148:intron 50.0%
!! TGCTGGATGGGTTATGGATG+CGG - chr1.2:75186926-75186945 MS.gene34148:intron 50.0%
CAGCAGTGCCAGTAGATGGA+AGG - chr1.2:75188243-75188262 MS.gene34148:intron 55.0%
CCAAGGAGACTCTATGCACC+TGG - chr1.2:75187955-75187974 MS.gene34148:intron 55.0%
CCAGGTGCATAGAGTCTCCT+TGG + chr1.2:75187958-75187977 None:intergenic 55.0%
CTGCAGCTACTCCTGATCCA+AGG + chr1.2:75187019-75187038 None:intergenic 55.0%
GAAAGAGGCCCAAAGGGCTT+TGG - chr1.2:75188325-75188344 MS.gene34148:intron 55.0%
GCTGGACATTCCCTTGGATC+AGG - chr1.2:75187005-75187024 MS.gene34148:intron 55.0%
GGCACTGCTGTTCTCACAAC+TGG + chr1.2:75188233-75188252 None:intergenic 55.0%
GGCTGTTAAAGGCTGCTGGA+TGG - chr1.2:75186913-75186932 MS.gene34148:intron 55.0%
TAGCGACAAAGACGTGGGAC+AGG + chr1.2:75186624-75186643 None:intergenic 55.0%
! AGACTAGTTGGGGGAGCTTG+TGG - chr1.2:75188727-75188746 MS.gene34148:intron 55.0%
! AGTAGCTGCAGCGTTGAGCA+TGG - chr1.2:75187028-75187047 MS.gene34148:intron 55.0%
! GGGTTGATTCCATAGCCACC+AGG + chr1.2:75186696-75186715 None:intergenic 55.0%
CACTGTCATGCCCTGCAGTG+TGG + chr1.2:75186566-75186585 None:intergenic 60.0%
! AGCTGCAGCGTTGAGCATGG+TGG - chr1.2:75187031-75187050 MS.gene34148:intron 60.0%
AACCCTCTTTGGGCGCCTCC+TGG - chr1.2:75186675-75186694 MS.gene34148:CDS 65.0%
ACCAAGGACAGCCACCCCGT+TGG - chr1.2:75187671-75187690 MS.gene34148:intron 65.0%
TCCAACGGGGTGGCTGTCCT+TGG + chr1.2:75187675-75187694 None:intergenic 65.0%
! TAGCTTGCGCTCGTTGGGCG+TGG - chr1.2:75186529-75186548 MS.gene34148:CDS 65.0%
!! CACCAGGAGGCGCCCAAAGA+GGG + chr1.2:75186680-75186699 None:intergenic 65.0%
CCTCTTTGGGCGCCTCCTGG+TGG - chr1.2:75186678-75186697 MS.gene34148:CDS 70.0%
TGGGCGCCTCCTGGTGGCTA+TGG - chr1.2:75186684-75186703 MS.gene34148:CDS 70.0%
!! CCACCAGGAGGCGCCCAAAG+AGG + chr1.2:75186681-75186700 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 75186483 75189640 75186483 ID=MS.gene34148
chr1.2 mRNA 75186483 75189640 75186483 ID=MS.gene34148.t1;Parent=MS.gene34148
chr1.2 exon 75188960 75189640 75188960 ID=MS.gene34148.t1.exon1;Parent=MS.gene34148.t1
chr1.2 CDS 75188960 75189640 75188960 ID=cds.MS.gene34148.t1;Parent=MS.gene34148.t1
chr1.2 exon 75188405 75188463 75188405 ID=MS.gene34148.t1.exon2;Parent=MS.gene34148.t1
chr1.2 CDS 75188405 75188463 75188405 ID=cds.MS.gene34148.t1;Parent=MS.gene34148.t1
chr1.2 exon 75188110 75188238 75188110 ID=MS.gene34148.t1.exon3;Parent=MS.gene34148.t1
chr1.2 CDS 75188110 75188238 75188110 ID=cds.MS.gene34148.t1;Parent=MS.gene34148.t1
chr1.2 exon 75187774 75187909 75187774 ID=MS.gene34148.t1.exon4;Parent=MS.gene34148.t1
chr1.2 CDS 75187774 75187909 75187774 ID=cds.MS.gene34148.t1;Parent=MS.gene34148.t1
chr1.2 exon 75186483 75186794 75186483 ID=MS.gene34148.t1.exon5;Parent=MS.gene34148.t1
chr1.2 CDS 75186483 75186794 75186483 ID=cds.MS.gene34148.t1;Parent=MS.gene34148.t1
Gene Sequence

>MS.gene34148

ATGTCGATTATCTGCGGTTTTCCTCTTGTTGAGTGTGTATATTGTTTAGCTTGCGCTCGTTGGGCGTGGAAAAGATGTCTCCACACTGCAGGGCATGACAGTGAAACTTGGGGATTTGCCGCAACAGAAGAATTTGAGCCTGTCCCACGTCTTTGTCGCTATATTTTAGCTGTGTATGAAGACGATCTTCGGAACCCTCTTTGGGCGCCTCCTGGTGGCTATGGAATCAACCCTGATTGGTTATTACTTAGAAAGACGTACAAAGATACAAGAGGACGGGCTCCACCATATATATTGTATCTTGATCATGATCATGCTGATATAGTTCTTGCTATAAGGGGACTAAATCTGGCAAAGGAGAGTGATTATGCTGTTCTGTTGGATAATAAATTAGGGAAGAGAAAGTTTGATGGGGGGTATGTTCACAATGGGCTGTTAAAGGCTGCTGGATGGGTTATGGATGCGGAGTGTGAAATTTTGAGGGAATTGGTAGAGAAGTATCCAAATTATACTCTTACATTTGCTGGACATTCCCTTGGATCAGGAGTAGCTGCAGCGTTGAGCATGGTGGTTGTGCAGAATCGAGATAGACTTGGAAATATTGAGAGGAAGAGAGTTAGGTGTTATGCTATTGCTCCTGCTAGATGTATGTCGCTTAATTTGGCAGTCAGATATGCAGATGTCATAAACTCTGTCGTGCTTCAGGCAAGTTATCAGTTTTTTTTTTGTTTAGTTGAAGAATCCTTGTCGTTCCTTTGAATATTTTTACATCTAGTAGAATGTGATGAATGTTAAATTAGAGCTTTTGCTGGCTAATTTGAAATCTTTCATGTGCCAGAGAGAAACAATTTATGGCGGATTTTCACGAACTTTAGTGTTTTACACGAGACAAATATTTTCCTGAAGTTTATTTTATGTGATAGCGACCTGATGGAAAAATGTTCGATTGTTGTTGTTTTATTATGAGAGATAGTAAAGGATTTGTTCTTATGAAAAGTGTGTTGCTTGCAGTTATTATATATATGTGCTCATAATAATTTTAAATTTTAGTTAATTTCAACTGTTGAGGTTAAGTGGCATGACAAACTTCTGCTTATACTCCCTTATACTTATTCAAGCTGACCTGATTCATGCTGAATCTTGTACTTTGAAGTTAATCGCTATTTTCCTTGGTTAGGATGACTTCTTACCAAGGACAGCCACCCCGTTGGAAGATATATTCAAGTCTCTTTTCTGGTATGTTGTAAGATCCCTAATACGACTAACTTCGTTATTATTGTGTTTGTTATGTACGACTAGAGTATGTTCTAGTATACTAAAGTCTAAGCAGTTAAATTAGTGTTGATGCTTCATAGCAATTTTATTGTTTTCATGAGAAGATATTGTTTTCTTGGATTTGCAGTTTGCCATGCTTATTGTGCTTAAGGTGCATGAGGGATACATGTATACCAGAGGAGAAGATGCTAAAAGATCCAAGGAGACTCTATGCACCTGGTCGCTTGTATCACATTGTTGAGAGAAAGCCTTTCAGGTATGGCTAAGGATTCAACAAAGTGCTTATACAGAACTGTAACCATAATTTTGGAAATTGCCTTCAGTTCAAACACAGTGTATCTTTTTGATTGTCTAGCATATGTTTATCTTATTTCTTCCATTGATATATAATAAAAAAAACATGCTTATACAGCCTTTTCGTTTGGCTTTACATTTATTAACACTGTTTCTGTGCAGATTGGGAAGGTTTCCTCCAGTTGTGAGAACAGCAGTGCCAGTAGATGGAAGGTTCGAGCATATCGTCCTTTCTTGTAATGCTACGTCAGATCATGCCATTATTTGGATAGAGAAAGAGGCCCAAAGGGCTTTGGATGTAAGTCTTTCAGCCTTGTAATTTTTGTACTTCATTCTTTGGTGGAATCAAACTTTTGTTTTTGTCTCTCTTTTTCTATGTGTTTGTGTCAACTATAATTAAGATTTGGCCTTAAGATTTGAAATTCTTAGAATAATACGAGAAATATGAATTTTGAGATGTAATCACTTCATATTTTATCAGCATCTTAGTTATAACAATTTCCATGTGTCTACCACATCTACCATGTGTAGGGAAATCAAAAAGAGCTTTTGACAATTTCAACCATATTTCCTTCAAGTATGCATACAAATTATCATAGGTTTTCCCCCTACTTATGGCCTATTTGGATTGCCTTATTACTTATTGGAGCTTATCTACTGGCATGAGCACTTGTGAGACTAGTTGGGGGAGCTTGTGGAAACAACTTATGACATGCCCATAAATTGTTTTCAGCTTCATAACTTTTCCATGATATTTTACGAAAACATATTGACTCTAATTTATCTTTTGTTATAGAAATATCTTATACATAAGCACTTAAGCTATTAGCGAAATCAAAAAGAGCTTTTGACAATTTCAACCATATGTCCTTCAAGTATGCATACAAATTATCATAGGTTTTCCCCCTACTTATGGCCTATTTGGATTGCCTTATTACTTATTGGAGCTTATCTACTGGCATGAGCACTTGTGAGACTAGTTGGGGGAGCTTGTGGAAACAACTTATGACATGCCCATAAATTGTTTTCAGCTTCATAACTTTTCCATGATATTTTACGAAAACATATTGACTCTAATTTATCTTTTGTTATAGAAATATCTTATACATAAGCACTTAAGCTATTAGAGCTTAACTAAATTGTTTATACAAACAATGTCTATATCAATTTGAGCTTCTTGTAGGCCTAAATAATCAATCAAGTTCCACTAATTTGGCATTCTATTTAATATATCAACGACATCTTTGCTATTTTGTTTATGCCACTGCCAATTGCAGTTGATGATGGAGAAAGATAATACCATGGAAGTACCTGTAAAGCAAATCATGCAGCGTCAGGAAACAATGACAAGACACGGCCAAGAATACAAAGCTGCATTACAAAGAGCGAAAACATTAGACATTCCACATGCTTTTACGCCACCATCAGAATATGGAACTTTTGATGAGGAAGGAGAGGAGAGTTCAAGGTCAGAGGCTGAGTCTTCTGTTAGTTCAACTAATAGGAGTACGGTTAACGAAAGCTGGGATGTATTGATTGAGCGTCTTTTTGATAAGGATGAGCATGGTCACATGGTGCTCAAGAGATGA
Protein sequence

>MS.gene34148.t1

MSIICGFPLVECVYCLACARWAWKRCLHTAGHDSETWGFAATEEFEPVPRLCRYILAVYEDDLRNPLWAPPGGYGINPDWLLLRKTYKDTRGRAPPYILYLDHDHADIVLAIRGLNLAKESDYAVLLDNKLGKRKFDGGYVHNGLLKAAGWVMDAECEILRELVEKYPNYTLTFAGHSLGSGVAAALSMVVVQNRDRLGNIERKRVRCYAIAPARCMSLNLAVRYADDDFLPRTATPLEDIFKSLFCLPCLLCLRCMRDTCIPEEKMLKDPRRLYAPGRLYHIVERKPFRLGRFPPVVRTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAQRALDLMMEKDNTMEVPVKQIMQRQETMTRHGQEYKAALQRAKTLDIPHAFTPPSEYGTFDEEGEESSRSEAESSVSSTNRSTVNESWDVLIERLFDKDEHGHMVLKR