AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene34210

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34210.t1 MTR_1g085460 53.942 241 105 2 19 254 18 257 3.92e-98 287
MS.gene34210.t1 MTR_7g111010 52.653 245 110 2 15 254 11 254 4.27e-98 287
MS.gene34210.t1 MTR_7g110990 52.263 243 104 3 17 254 4 239 2.63e-95 280
MS.gene34210.t1 MTR_1g026020 51.984 252 111 4 11 254 6 255 3.24e-95 280
MS.gene34210.t1 MTR_3g435430 54.274 234 97 5 21 249 15 243 1.84e-89 265
MS.gene34210.t1 MTR_5g041700 56.889 225 87 5 30 249 24 243 6.69e-88 261
MS.gene34210.t1 MTR_4g081950 50.781 256 110 7 3 249 31 279 2.47e-87 261
MS.gene34210.t1 MTR_3g094040 51.190 252 112 6 4 249 3 249 7.46e-87 259
MS.gene34210.t1 MTR_3g094060 50.794 252 113 6 4 249 3 249 3.51e-86 257
MS.gene34210.t1 MTR_5g075320 50.598 251 111 7 6 248 1 246 5.26e-86 256
MS.gene34210.t1 MTR_7g011060 53.975 239 98 6 15 249 6 236 8.58e-86 255
MS.gene34210.t1 MTR_3g466790 50.800 250 109 6 8 248 5 249 1.08e-83 251
MS.gene34210.t1 MTR_5g079950 48.077 260 123 6 1 252 1 256 2.33e-82 247
MS.gene34210.t1 MTR_3g466760 50.820 244 104 7 15 248 18 255 1.13e-81 246
MS.gene34210.t1 MTR_7g061720 50.439 228 103 5 31 252 32 255 4.23e-81 244
MS.gene34210.t1 MTR_2g097030 46.484 256 125 6 5 252 6 257 1.06e-79 241
MS.gene34210.t1 MTR_7g013150 47.581 248 119 6 11 254 13 253 3.09e-78 237
MS.gene34210.t1 MTR_6g012520 55.752 226 92 5 30 255 27 244 8.08e-77 233
MS.gene34210.t1 MTR_4g063975 45.098 255 130 5 4 252 5 255 3.14e-75 229
MS.gene34210.t1 MTR_7g013140 46.311 244 122 6 15 254 13 251 3.19e-75 229
MS.gene34210.t1 MTR_7g013120 46.721 244 122 5 15 254 14 253 4.98e-75 228
MS.gene34210.t1 MTR_8g058990 48.361 244 117 6 15 254 15 253 1.89e-73 224
MS.gene34210.t1 MTR_4g124340 44.828 232 115 6 28 252 58 283 5.20e-71 219
MS.gene34210.t1 MTR_8g464350 46.774 248 123 6 11 254 12 254 5.40e-71 218
MS.gene34210.t1 MTR_4g102450 47.458 236 112 6 27 255 32 262 1.32e-70 218
MS.gene34210.t1 MTR_0040s0140 44.882 254 126 7 1 248 6 251 6.50e-70 216
MS.gene34210.t1 MTR_5g011210 46.552 232 112 6 31 255 37 263 3.50e-69 214
MS.gene34210.t1 MTR_2g090255 44.082 245 127 5 11 249 8 248 6.13e-69 213
MS.gene34210.t1 MTR_5g076950 46.552 232 111 5 31 255 41 266 3.60e-67 209
MS.gene34210.t1 MTR_3g064510 46.552 232 107 7 31 249 53 280 2.27e-66 207
MS.gene34210.t1 MTR_0008s0120 34.426 244 103 4 11 254 13 199 1.53e-39 136
MS.gene34210.t1 MTR_2g090270 31.660 259 152 9 11 254 10 258 2.37e-31 116
MS.gene34210.t1 MTR_0122s0020 35.745 235 131 8 31 255 41 265 8.28e-31 115
MS.gene34210.t1 MTR_2g090265 31.783 258 147 10 15 254 10 256 1.21e-28 109
MS.gene34210.t1 MTR_6g012500 50.485 103 50 1 153 255 181 282 1.99e-28 109
MS.gene34210.t1 MTR_4g028640 33.210 271 145 12 6 254 8 264 9.84e-26 102
MS.gene34210.t1 MTR_0809s0010 31.224 237 137 10 31 254 47 270 2.18e-21 90.5
MS.gene34210.t1 MTR_0118s0070 31.140 228 135 8 36 250 53 271 9.63e-21 89.0
MS.gene34210.t1 MTR_5g013440 29.730 259 150 12 9 254 12 251 2.05e-18 82.0
MS.gene34210.t1 MTR_0122s0020 35.632 174 92 8 31 194 41 204 5.94e-18 80.1
MS.gene34210.t1 MTR_1g085365 55.556 54 24 0 198 251 4 57 3.96e-13 63.2
MS.gene34210.t1 MTR_4g099370 26.070 257 155 12 14 254 13 250 1.03e-12 66.2
MS.gene34210.t1 MTR_4g075790 27.660 235 141 10 33 254 25 243 1.49e-11 63.2
MS.gene34210.t1 MTR_4g098930 26.038 265 159 10 4 253 9 251 1.84e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34210.t1 AT3G15370 66.122 245 83 0 11 255 7 251 5.30e-123 350
MS.gene34210.t1 AT3G15370 59.341 273 81 1 11 253 7 279 3.03e-116 334
MS.gene34210.t1 AT4G01630 52.521 238 107 2 22 254 19 255 8.29e-96 281
MS.gene34210.t1 AT1G20190 54.393 239 99 4 24 254 16 252 6.22e-90 266
MS.gene34210.t1 AT1G69530 54.321 243 98 6 15 249 8 245 1.93e-88 263
MS.gene34210.t1 AT1G69530 54.321 243 98 6 15 249 8 245 1.93e-88 263
MS.gene34210.t1 AT2G40610 51.653 242 108 4 12 248 11 248 2.00e-88 263
MS.gene34210.t1 AT1G69530 54.321 243 98 6 15 249 8 245 3.27e-88 263
MS.gene34210.t1 AT5G56320 52.500 240 104 5 15 249 13 247 8.17e-88 261
MS.gene34210.t1 AT5G56320 52.500 240 104 5 15 249 13 247 8.17e-88 261
MS.gene34210.t1 AT2G03090 53.150 254 103 7 1 249 6 248 1.75e-87 260
MS.gene34210.t1 AT5G56320 52.500 240 104 5 15 249 13 247 3.55e-87 261
MS.gene34210.t1 AT2G39700 50.000 250 113 6 11 252 9 254 3.74e-83 249
MS.gene34210.t1 AT3G29030 52.968 219 96 4 31 249 38 249 4.41e-83 249
MS.gene34210.t1 AT5G05290 48.571 245 116 5 11 249 11 251 8.30e-83 248
MS.gene34210.t1 AT3G55500 48.263 259 123 6 1 252 3 257 5.90e-82 246
MS.gene34210.t1 AT5G02260 50.439 228 103 5 31 252 32 255 2.63e-81 245
MS.gene34210.t1 AT2G37640 50.877 228 102 5 31 252 36 259 1.25e-79 241
MS.gene34210.t1 AT1G26770 50.000 246 113 5 9 249 4 244 2.74e-79 239
MS.gene34210.t1 AT1G26770 50.000 246 113 5 9 249 14 254 5.41e-79 239
MS.gene34210.t1 AT2G28950 47.755 245 116 6 16 252 14 254 1.68e-76 233
MS.gene34210.t1 AT5G39280 49.780 227 106 5 31 254 54 275 2.70e-76 233
MS.gene34210.t1 AT5G39300 48.899 227 108 5 31 254 55 276 3.00e-76 233
MS.gene34210.t1 AT1G20190 56.186 194 78 3 66 254 2 193 2.39e-74 225
MS.gene34210.t1 AT1G69530 55.122 205 79 6 15 211 8 207 3.25e-73 224
MS.gene34210.t1 AT1G69530 55.122 205 79 6 15 211 8 207 3.84e-73 223
MS.gene34210.t1 AT5G39290 49.339 227 107 5 31 254 58 279 8.75e-73 224
MS.gene34210.t1 AT5G39270 49.339 227 107 5 31 254 58 279 1.14e-72 223
MS.gene34210.t1 AT2G37640 51.185 211 93 5 48 252 28 234 2.15e-72 221
MS.gene34210.t1 AT1G12560 43.233 266 134 7 1 254 1 261 8.81e-72 221
MS.gene34210.t1 AT3G03220 47.345 226 106 5 31 249 40 259 6.49e-68 211
MS.gene34210.t1 AT1G62980 43.913 230 119 5 31 254 31 256 4.66e-65 203
MS.gene34210.t1 AT4G38210 45.200 250 124 7 14 255 11 255 8.30e-65 202
MS.gene34210.t1 AT5G39310 43.777 233 122 6 26 254 85 312 2.36e-64 203
MS.gene34210.t1 AT5G39260 44.444 225 117 6 31 254 45 262 7.44e-62 195
MS.gene34210.t1 AT4G28250 37.037 270 138 11 3 254 5 260 7.83e-39 136
MS.gene34210.t1 AT2G20750 37.344 241 133 9 25 254 34 267 3.12e-35 127
MS.gene34210.t1 AT4G28250 35.316 269 138 11 3 254 5 254 6.78e-34 123
MS.gene34210.t1 AT1G65680 35.294 238 129 11 31 254 47 273 1.30e-26 104
MS.gene34210.t1 AT2G20750 38.418 177 92 8 25 191 34 203 4.69e-24 96.7
MS.gene34210.t1 AT4G28250 33.971 209 103 10 3 195 5 194 1.33e-22 92.4
MS.gene34210.t1 AT4G28250 34.634 205 99 10 3 191 5 190 2.46e-22 91.7
MS.gene34210.t1 AT1G65681 30.594 219 131 10 47 254 14 222 5.79e-19 83.2
MS.gene34210.t1 AT3G60570 26.360 239 146 8 31 254 34 257 1.41e-17 80.1
MS.gene34210.t1 AT4G17030 26.894 264 154 11 4 251 6 246 3.24e-14 70.5
MS.gene34210.t1 AT3G60570 25.792 221 134 8 49 254 4 209 1.03e-13 68.6
MS.gene34210.t1 AT3G45960 29.000 200 129 7 62 254 6 199 1.43e-12 65.5
MS.gene34210.t1 AT3G45960 29.000 200 129 7 62 254 54 247 2.00e-12 65.9
MS.gene34210.t1 AT4G38400 26.500 200 134 7 62 254 55 248 1.23e-11 63.5

Find 63 sgRNAs with CRISPR-Local

Find 141 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTGCCAATGCACAGAAAT+TGG 0.147949 8.4:+48189849 MS.gene34210:CDS
AACGCTCATGCAACGTTCTA+TGG 0.182142 8.4:+48186715 MS.gene34210:CDS
GACTTCTAAGACACCATTTC+GGG 0.244010 8.4:-48188569 None:intergenic
GTTTCTTCCAATTGGAGATT+TGG 0.274322 8.4:+48189968 MS.gene34210:CDS
GAAACTAATAGAATTGATTA+TGG 0.275627 8.4:-48190006 None:intergenic
TTCAAGATTACTTTGGTTGA+TGG 0.313364 8.4:+48189920 MS.gene34210:CDS
CACCATCCTCCATGATTGTT+TGG 0.340496 8.4:-48188627 None:intergenic
GATATCTTTCATGCTGGGTT+TGG 0.347405 8.4:+48188456 MS.gene34210:CDS
TGGCAACCAAGCTCCACTTC+TGG 0.354563 8.4:-48189834 None:intergenic
TCAATGTAGTTTCTTCCAAT+TGG 0.355197 8.4:+48189960 MS.gene34210:CDS
GTTGCCAATGCACAGAAATT+GGG 0.356433 8.4:+48189850 MS.gene34210:CDS
ACAGAAATTGGGGAGCTAAT+TGG 0.358167 8.4:+48189861 MS.gene34210:CDS
CGACTTCTAAGACACCATTT+CGG 0.364267 8.4:-48188570 None:intergenic
AAGGCTGGTTGGACTTTGAT+TGG 0.372747 8.4:-48186740 None:intergenic
GGCAAAGCAGTGCAGATCTA+AGG 0.383218 8.4:+48189882 MS.gene34210:CDS
TTCTACCTTCTATAAAGAAC+AGG 0.400951 8.4:-48188729 None:intergenic
TTGTTCCTGTTCTTTATAGA+AGG 0.410739 8.4:+48188724 MS.gene34210:CDS
TATGATGATATCTTTCATGC+TGG 0.420228 8.4:+48188450 MS.gene34210:CDS
GCAAAAGAAGAGGAGGAGTT+AGG 0.422267 8.4:+48189720 MS.gene34210:CDS
ACTATAAAATCGACCCGAAA+TGG 0.429332 8.4:+48188556 MS.gene34210:CDS
TAATCTTGAATGAGAGTCTC+TGG 0.444856 8.4:-48189907 None:intergenic
TTCAGAGATGGTGAGGTTTG+TGG 0.448953 8.4:+48188510 MS.gene34210:CDS
ACTCTCATTCAAGATTACTT+TGG 0.449267 8.4:+48189913 MS.gene34210:CDS
AGGAGTTAGGTTCACATTGA+AGG 0.456036 8.4:+48189733 MS.gene34210:CDS
ACGCTCATGCAACGTTCTAT+GGG 0.460595 8.4:+48186716 MS.gene34210:CDS
ATGATGATATCTTTCATGCT+GGG 0.461474 8.4:+48188451 MS.gene34210:CDS
GCTCCCCAATTTCTGTGCAT+TGG 0.463834 8.4:-48189854 None:intergenic
AAACTAATAGAATTGATTAT+GGG 0.477188 8.4:-48190005 None:intergenic
TGTTTGTCCAAATCTCCAAT+TGG 0.490797 8.4:-48189975 None:intergenic
GTGAAGGGAGTGTGGATAAG+AGG 0.501955 8.4:+48189809 MS.gene34210:CDS
ATAGTATGTCATACCAAGGC+TGG 0.505913 8.4:-48186755 None:intergenic
ATAACAAACGTGGGTGGTAG+CGG 0.518408 8.4:+48189782 MS.gene34210:CDS
GGGTGGTAGCGGTGATGTGA+AGG 0.525724 8.4:+48189793 MS.gene34210:CDS
GGAGTTAGGTTCACATTGAA+GGG 0.526521 8.4:+48189734 MS.gene34210:CDS
TTAAATGAAGAAATCATAAG+AGG 0.526570 8.4:-48186625 None:intergenic
AATCAAAGTCCAACCAGCCT+TGG 0.527653 8.4:+48186742 MS.gene34210:CDS
ATTGCACGACAAGGCAATGA+AGG 0.531708 8.4:+48188699 MS.gene34210:CDS
TGGATAAGAGGTTCCAGAAG+TGG 0.541107 8.4:+48189821 MS.gene34210:CDS
CGTGCAATGCGAAAGAAAGC+AGG 0.542098 8.4:-48188684 None:intergenic
TTCTTTCGCATTGCACGACA+AGG 0.545521 8.4:+48188690 MS.gene34210:CDS
GAGGTTCCAGAAGTGGAGCT+TGG 0.547406 8.4:+48189828 MS.gene34210:CDS
CATCCTCCATGATTGTTTGG+AGG 0.550332 8.4:-48188624 None:intergenic
AGAATCCACGTTGAAGCTAA+CGG 0.575394 8.4:+48186685 MS.gene34210:CDS
TTGCCAATGCACAGAAATTG+GGG 0.577155 8.4:+48189851 MS.gene34210:CDS
GCGGTGATGTGAAGGGAGTG+TGG 0.578538 8.4:+48189801 MS.gene34210:CDS
TCCACGTTGAAGCTAACGGT+TGG 0.587064 8.4:+48186689 MS.gene34210:CDS
CTCCAAACAATCATGGAGGA+TGG 0.600779 8.4:+48188625 MS.gene34210:CDS
GGTGGTAGCGGTGATGTGAA+GGG 0.604265 8.4:+48189794 MS.gene34210:CDS
TATGTCATACCAAGGCTGGT+TGG 0.607702 8.4:-48186751 None:intergenic
GCATGGACATATCAAAGTGA+TGG 0.615233 8.4:-48188662 None:intergenic
AGGGTAGCATGCAAAAGAAG+AGG 0.620539 8.4:+48189710 MS.gene34210:intron
GTAGCATGCAAAAGAAGAGG+AGG 0.621264 8.4:+48189713 MS.gene34210:CDS
GACATATCAAAGTGATGGTG+TGG 0.621785 8.4:-48188657 None:intergenic
ATATCAAAGTGATGGTGTGG+AGG 0.623230 8.4:-48188654 None:intergenic
TTTGGTTGATGGTAAAACAC+TGG 0.629207 8.4:+48189931 MS.gene34210:CDS
CGCTCATGCAACGTTCTATG+GGG 0.631588 8.4:+48186717 MS.gene34210:CDS
AATGCGAAAGAAAGCAGGCA+TGG 0.655376 8.4:-48188679 None:intergenic
GGGTCAATCAAATTTCAACA+TGG 0.676126 8.4:+48189754 MS.gene34210:CDS
TGTCCTCCAAACAATCATGG+AGG 0.680110 8.4:+48188621 MS.gene34210:CDS
CATGGTGATGATAACAAACG+TGG 0.683408 8.4:+48189772 MS.gene34210:CDS
GCCAACCGTTAGCTTCAACG+TGG 0.691827 8.4:-48186690 None:intergenic
GTGATGATAACAAACGTGGG+TGG 0.768037 8.4:+48189776 MS.gene34210:CDS
ATGGTGATGATAACAAACGT+GGG 0.793586 8.4:+48189773 MS.gene34210:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAGAAGAATATTTA+TGG + chr8.4:48189494-48189513 MS.gene34210:intron 10.0%
!! AAAAAAAGAAGAATATTTAT+GGG + chr8.4:48189495-48189514 MS.gene34210:intron 10.0%
!! AATGCATATAAATTATAATA+TGG + chr8.4:48189438-48189457 MS.gene34210:intron 10.0%
!! TATAAATAAAAAATCTAATG+AGG + chr8.4:48189178-48189197 MS.gene34210:intron 10.0%
!! TGTTATATTAAATTATCATA+AGG - chr8.4:48188383-48188402 None:intergenic 10.0%
!! TTCTTATCTATAATATTTAT+TGG + chr8.4:48186818-48186837 MS.gene34210:intron 10.0%
!!! TTTCTTCATTTAATTTTATT+TGG + chr8.4:48186635-48186654 MS.gene34210:CDS 10.0%
!! AAAAAGTTGTTGTAAAAAAA+TGG - chr8.4:48187388-48187407 None:intergenic 15.0%
!! AAGTACATGTATATATAATA+CGG - chr8.4:48189686-48189705 None:intergenic 15.0%
!! AATAATAGTTCTCAATAAAA+AGG - chr8.4:48187358-48187377 None:intergenic 15.0%
!! AATAGTAATAAAATTCAACA+TGG + chr8.4:48187602-48187621 MS.gene34210:intron 15.0%
!! ATAATAGTTCTCAATAAAAA+GGG - chr8.4:48187357-48187376 None:intergenic 15.0%
!! ATAATTTATACACACATATA+AGG - chr8.4:48187081-48187100 None:intergenic 15.0%
!! ATTAGTCAAAGAATTAAATA+TGG - chr8.4:48189126-48189145 None:intergenic 15.0%
!! CATAAATTTACAAAGAAAAA+AGG - chr8.4:48187475-48187494 None:intergenic 15.0%
!!! CTTTTTTCTTTGTAAATTTA+TGG + chr8.4:48187473-48187492 MS.gene34210:intron 15.0%
!!! TAGTTTTAAATTTATCAAGA+AGG + chr8.4:48187010-48187029 MS.gene34210:intron 15.0%
!!! TCTTAAATTTTTGATTGTAT+AGG + chr8.4:48188918-48188937 MS.gene34210:intron 15.0%
!!! TGAAATTTTTATATGTTTTG+TGG + chr8.4:48187102-48187121 MS.gene34210:intron 15.0%
!!! TGGTCTATATAAAATATTTT+TGG - chr8.4:48187714-48187733 None:intergenic 15.0%
!! AAATACCATAGTTGAATATA+TGG + chr8.4:48188235-48188254 MS.gene34210:intron 20.0%
!! AATTAGACTAATGTAACATT+TGG - chr8.4:48188009-48188028 None:intergenic 20.0%
!! AATTCTTTGACTAATACTTA+AGG + chr8.4:48189131-48189150 MS.gene34210:intron 20.0%
!! AGGTTCTTAATATTAAACAA+AGG - chr8.4:48188363-48188382 None:intergenic 20.0%
!! ATTTATGTAAAGATTTAGAG+AGG - chr8.4:48187737-48187756 None:intergenic 20.0%
!! CATCATGTATCATATATAAA+GGG - chr8.4:48189334-48189353 None:intergenic 20.0%
!! GATGATCTAATATAAAGATA+TGG + chr8.4:48188150-48188169 MS.gene34210:intron 20.0%
!! GGTTCTTAATATTAAACAAA+GGG - chr8.4:48188362-48188381 None:intergenic 20.0%
!! TAATAGTTCTCAATAAAAAG+GGG - chr8.4:48187356-48187375 None:intergenic 20.0%
!! TCATCATGTATCATATATAA+AGG - chr8.4:48189335-48189354 None:intergenic 20.0%
!! TTAAATGAAGAAATCATAAG+AGG - chr8.4:48186628-48186647 None:intergenic 20.0%
!! TTAGTCCATAATTATAATGT+AGG + chr8.4:48189655-48189674 MS.gene34210:intron 20.0%
!! TTATATATGATACATGATGA+GGG + chr8.4:48189335-48189354 MS.gene34210:intron 20.0%
!! TTGTTTGTCATTAAGATATT+GGG - chr8.4:48189262-48189281 None:intergenic 20.0%
!! TTTATATATGATACATGATG+AGG + chr8.4:48189334-48189353 MS.gene34210:intron 20.0%
!! TTTGTTTGTCATTAAGATAT+TGG - chr8.4:48189263-48189282 None:intergenic 20.0%
!!! AAACTATAAATCTCTTTTTG+AGG - chr8.4:48189465-48189484 None:intergenic 20.0%
!!! TGTGATTATGACAATATTTT+AGG + chr8.4:48188414-48188433 MS.gene34210:intron 20.0%
!!! TTTGATATTTTCAATCAGTT+TGG + chr8.4:48188027-48188046 MS.gene34210:intron 20.0%
!!! TTTTATTTGGTGTCTTTTAT+TGG + chr8.4:48186648-48186667 MS.gene34210:CDS 20.0%
!!! TTTTCTTAGTGCATTAATTA+TGG + chr8.4:48186851-48186870 MS.gene34210:intron 20.0%
! AAAAGAAGAATATTTATGGG+TGG + chr8.4:48189498-48189517 MS.gene34210:intron 25.0%
! AAAATCCACTATTTCACTAA+TGG + chr8.4:48189583-48189602 MS.gene34210:intron 25.0%
! AATGAGTGTAAAGTAATATC+AGG + chr8.4:48188179-48188198 MS.gene34210:intron 25.0%
! ATATATACATGTACTTGAAC+AGG + chr8.4:48189690-48189709 MS.gene34210:intron 25.0%
! GAGTAAATCATGAATGTATA+AGG + chr8.4:48188058-48188077 MS.gene34210:intron 25.0%
! GATTACCATATATTCAACTA+TGG - chr8.4:48188243-48188262 None:intergenic 25.0%
! TATATACATGTACTTGAACA+GGG + chr8.4:48189691-48189710 MS.gene34210:intron 25.0%
!! TCATACTTGTAAGTTTTACA+TGG + chr8.4:48187686-48187705 MS.gene34210:intron 25.0%
!! TTTTATAGTCACATATGACT+TGG - chr8.4:48188545-48188564 None:intergenic 25.0%
!!! GATTATGACAATATTTTAGG+AGG + chr8.4:48188417-48188436 MS.gene34210:intron 25.0%
!!! GGAGGTAGAAATTTATTTTT+CGG - chr8.4:48188981-48189000 None:intergenic 25.0%
!!! TAACGTCATATCTTTTTTAG+TGG - chr8.4:48188304-48188323 None:intergenic 25.0%
ACTCTCATTCAAGATTACTT+TGG + chr8.4:48189913-48189932 MS.gene34210:CDS 30.0%
AGTAAACTCTTACTACTAGT+TGG + chr8.4:48189391-48189410 MS.gene34210:intron 30.0%
ATGATGATATCTTTCATGCT+GGG + chr8.4:48188451-48188470 MS.gene34210:CDS 30.0%
CATGTATATATAATACGGTG+AGG - chr8.4:48189681-48189700 None:intergenic 30.0%
CTTAATAGTATGTCATACCA+AGG - chr8.4:48186762-48186781 None:intergenic 30.0%
CTTTGTCATCTCTCTTTAAT+GGG + chr8.4:48188772-48188791 MS.gene34210:intron 30.0%
GATAACCATTAGTGAAATAG+TGG - chr8.4:48189591-48189610 None:intergenic 30.0%
TATGATGATATCTTTCATGC+TGG + chr8.4:48188450-48188469 MS.gene34210:CDS 30.0%
TATGTAAAGATTTAGAGAGG+TGG - chr8.4:48187734-48187753 None:intergenic 30.0%
TATTGAAGACACTATGAAAG+TGG + chr8.4:48189212-48189231 MS.gene34210:intron 30.0%
TCAATGTAGTTTCTTCCAAT+TGG + chr8.4:48189960-48189979 MS.gene34210:CDS 30.0%
TCTTTGTCATCTCTCTTTAA+TGG + chr8.4:48188771-48188790 MS.gene34210:intron 30.0%
TGAGGCCTACATTATAATTA+TGG - chr8.4:48189663-48189682 None:intergenic 30.0%
TTCAAGATTACTTTGGTTGA+TGG + chr8.4:48189920-48189939 MS.gene34210:CDS 30.0%
TTCAATCAGTTTGGATTGAA+AGG + chr8.4:48188036-48188055 MS.gene34210:intron 30.0%
TTCTACCTTCTATAAAGAAC+AGG - chr8.4:48188732-48188751 None:intergenic 30.0%
TTGTTCCTGTTCTTTATAGA+AGG + chr8.4:48188724-48188743 MS.gene34210:CDS 30.0%
! TTTTCTTAACTAATGTCTCG+AGG - chr8.4:48189022-48189041 None:intergenic 30.0%
AACGAAAAAGTTTACGTGTG+TGG - chr8.4:48189637-48189656 None:intergenic 35.0%
ACTATAAAATCGACCCGAAA+TGG + chr8.4:48188556-48188575 MS.gene34210:CDS 35.0%
GGGTCAATCAAATTTCAACA+TGG + chr8.4:48189754-48189773 MS.gene34210:CDS 35.0%
GTTTCTTCCAATTGGAGATT+TGG + chr8.4:48189968-48189987 MS.gene34210:CDS 35.0%
GTTTGGAGGACAAAAATTTG+TGG - chr8.4:48188613-48188632 None:intergenic 35.0%
TAATCTTGAATGAGAGTCTC+TGG - chr8.4:48189910-48189929 None:intergenic 35.0%
TGTTTGTCCAAATCTCCAAT+TGG - chr8.4:48189978-48189997 None:intergenic 35.0%
TTTACTGCTCTTACAGAGAT+CGG - chr8.4:48189377-48189396 None:intergenic 35.0%
! ATGGTGATGATAACAAACGT+GGG + chr8.4:48189773-48189792 MS.gene34210:CDS 35.0%
! TTTTGTCCTCCAAACAATCA+TGG + chr8.4:48188618-48188637 MS.gene34210:CDS 35.0%
!! AATATTTTAGGAGGAGCTTG+TGG + chr8.4:48188426-48188445 MS.gene34210:intron 35.0%
!! TTTGGTTGATGGTAAAACAC+TGG + chr8.4:48189931-48189950 MS.gene34210:CDS 35.0%
ACAGAAATTGGGGAGCTAAT+TGG + chr8.4:48189861-48189880 MS.gene34210:CDS 40.0%
AGAATCCACGTTGAAGCTAA+CGG + chr8.4:48186685-48186704 MS.gene34210:CDS 40.0%
AGGAGTTAGGTTCACATTGA+AGG + chr8.4:48189733-48189752 MS.gene34210:CDS 40.0%
ATAGTATGTCATACCAAGGC+TGG - chr8.4:48186758-48186777 None:intergenic 40.0%
ATCACAAAAGCTCCTGCTAT+AGG + chr8.4:48189608-48189627 MS.gene34210:intron 40.0%
GATATCTTTCATGCTGGGTT+TGG + chr8.4:48188456-48188475 MS.gene34210:CDS 40.0%
GCATGGACATATCAAAGTGA+TGG - chr8.4:48188665-48188684 None:intergenic 40.0%
GGAGTTAGGTTCACATTGAA+GGG + chr8.4:48189734-48189753 MS.gene34210:CDS 40.0%
GTTGCCAATGCACAGAAATT+GGG + chr8.4:48189850-48189869 MS.gene34210:CDS 40.0%
TCACAAAAGCTCCTGCTATA+GGG + chr8.4:48189609-48189628 MS.gene34210:intron 40.0%
TCTCATCTCTTTCTGTCTCA+AGG - chr8.4:48188104-48188123 None:intergenic 40.0%
TTGCCAATGCACAGAAATTG+GGG + chr8.4:48189851-48189870 MS.gene34210:CDS 40.0%
! AGCACTATGCTTTTCAGAGA+TGG + chr8.4:48188498-48188517 MS.gene34210:CDS 40.0%
! ATATCAAAGTGATGGTGTGG+AGG - chr8.4:48188657-48188676 None:intergenic 40.0%
! CATGGTGATGATAACAAACG+TGG + chr8.4:48189772-48189791 MS.gene34210:CDS 40.0%
! CTCTCTTTAATGGGCAATGA+TGG + chr8.4:48188781-48188800 MS.gene34210:intron 40.0%
! GACATATCAAAGTGATGGTG+TGG - chr8.4:48188660-48188679 None:intergenic 40.0%
! TATGCTTTTCAGAGATGGTG+AGG + chr8.4:48188503-48188522 MS.gene34210:CDS 40.0%
!! CGACTTCTAAGACACCATTT+CGG - chr8.4:48188573-48188592 None:intergenic 40.0%
!! GACTTCTAAGACACCATTTC+GGG - chr8.4:48188572-48188591 None:intergenic 40.0%
AACGCTCATGCAACGTTCTA+TGG + chr8.4:48186715-48186734 MS.gene34210:CDS 45.0%
AACTAATGTCTCGAGGACAC+TGG - chr8.4:48189015-48189034 None:intergenic 45.0%
AATCAAAGTCCAACCAGCCT+TGG + chr8.4:48186742-48186761 MS.gene34210:CDS 45.0%
AATGCGAAAGAAAGCAGGCA+TGG - chr8.4:48188682-48188701 None:intergenic 45.0%
ACGCTCATGCAACGTTCTAT+GGG + chr8.4:48186716-48186735 MS.gene34210:CDS 45.0%
AGGGTAGCATGCAAAAGAAG+AGG + chr8.4:48189710-48189729 MS.gene34210:intron 45.0%
ATAACAAACGTGGGTGGTAG+CGG + chr8.4:48189782-48189801 MS.gene34210:CDS 45.0%
ATTGCACGACAAGGCAATGA+AGG + chr8.4:48188699-48188718 MS.gene34210:CDS 45.0%
CACCATCCTCCATGATTGTT+TGG - chr8.4:48188630-48188649 None:intergenic 45.0%
CATCCTCCATGATTGTTTGG+AGG - chr8.4:48188627-48188646 None:intergenic 45.0%
CTCCAAACAATCATGGAGGA+TGG + chr8.4:48188625-48188644 MS.gene34210:CDS 45.0%
GCAAAAGAAGAGGAGGAGTT+AGG + chr8.4:48189720-48189739 MS.gene34210:CDS 45.0%
GGTTGCCAATGCACAGAAAT+TGG + chr8.4:48189849-48189868 MS.gene34210:CDS 45.0%
GTAGCATGCAAAAGAAGAGG+AGG + chr8.4:48189713-48189732 MS.gene34210:CDS 45.0%
GTGATGATAACAAACGTGGG+TGG + chr8.4:48189776-48189795 MS.gene34210:CDS 45.0%
TATGTCATACCAAGGCTGGT+TGG - chr8.4:48186754-48186773 None:intergenic 45.0%
TGGATAAGAGGTTCCAGAAG+TGG + chr8.4:48189821-48189840 MS.gene34210:CDS 45.0%
TGTCCTCCAAACAATCATGG+AGG + chr8.4:48188621-48188640 MS.gene34210:CDS 45.0%
TTCAGAGATGGTGAGGTTTG+TGG + chr8.4:48188510-48188529 MS.gene34210:CDS 45.0%
TTCTTTCGCATTGCACGACA+AGG + chr8.4:48188690-48188709 MS.gene34210:CDS 45.0%
! AAGGCTGGTTGGACTTTGAT+TGG - chr8.4:48186743-48186762 None:intergenic 45.0%
! ACACTGGTTACTTAGCTTGG+AGG - chr8.4:48188999-48189018 None:intergenic 45.0%
! AGGACACTGGTTACTTAGCT+TGG - chr8.4:48189002-48189021 None:intergenic 45.0%
!! AAAATATATAATATACTAAA+TGG + chr8.4:48187837-48187856 MS.gene34210:intron 5.0%
!! TAAAATTAAATAATTAACTT+TGG + chr8.4:48187644-48187663 MS.gene34210:intron 5.0%
CGCTCATGCAACGTTCTATG+GGG + chr8.4:48186717-48186736 MS.gene34210:CDS 50.0%
CGTGCAATGCGAAAGAAAGC+AGG - chr8.4:48188687-48188706 None:intergenic 50.0%
GCTCCCCAATTTCTGTGCAT+TGG - chr8.4:48189857-48189876 None:intergenic 50.0%
GGCAAAGCAGTGCAGATCTA+AGG + chr8.4:48189882-48189901 MS.gene34210:CDS 50.0%
TCCACGTTGAAGCTAACGGT+TGG + chr8.4:48186689-48186708 MS.gene34210:CDS 50.0%
! GTGAAGGGAGTGTGGATAAG+AGG + chr8.4:48189809-48189828 MS.gene34210:CDS 50.0%
CGTGTGTGGATCCCTATAGC+AGG - chr8.4:48189623-48189642 None:intergenic 55.0%
GAGGTTCCAGAAGTGGAGCT+TGG + chr8.4:48189828-48189847 MS.gene34210:CDS 55.0%
TGGCAACCAAGCTCCACTTC+TGG - chr8.4:48189837-48189856 None:intergenic 55.0%
! GCCAACCGTTAGCTTCAACG+TGG - chr8.4:48186693-48186712 None:intergenic 55.0%
!! GGTGGTAGCGGTGATGTGAA+GGG + chr8.4:48189794-48189813 MS.gene34210:CDS 55.0%
GCGGTGATGTGAAGGGAGTG+TGG + chr8.4:48189801-48189820 MS.gene34210:CDS 60.0%
!! GGGTGGTAGCGGTGATGTGA+AGG + chr8.4:48189793-48189812 MS.gene34210:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 48186619 48190024 48186619 ID=MS.gene34210
chr8.4 mRNA 48186619 48190024 48186619 ID=MS.gene34210.t1;Parent=MS.gene34210
chr8.4 exon 48186619 48186763 48186619 ID=MS.gene34210.t1.exon1;Parent=MS.gene34210.t1
chr8.4 CDS 48186619 48186763 48186619 ID=cds.MS.gene34210.t1;Parent=MS.gene34210.t1
chr8.4 exon 48188436 48188745 48188436 ID=MS.gene34210.t1.exon2;Parent=MS.gene34210.t1
chr8.4 CDS 48188436 48188745 48188436 ID=cds.MS.gene34210.t1;Parent=MS.gene34210.t1
chr8.4 exon 48189712 48190024 48189712 ID=MS.gene34210.t1.exon3;Parent=MS.gene34210.t1
chr8.4 CDS 48189712 48190024 48189712 ID=cds.MS.gene34210.t1;Parent=MS.gene34210.t1
Gene Sequence

>MS.gene34210

ATGGGACCTCTTATGATTTCTTCATTTAATTTTATTTGGTGTCTTTTATTGGTTTTATGTGCAGTTAGAATCCACGTTGAAGCTAACGGTTGGCTTAACGCTCATGCAACGTTCTATGGGGCCAATCAAAGTCCAACCAGCCTTGGTATGACATACTATTAAGTTTTTAGAGATCTCAAAATACATATTAACTTTTTATTTCTTATCTATAATATTTATTGGTGTTTCAATTTTTTCTTAGTGCATTAATTATGGTTTAGATATTAACTAGTTTACATACATACATACATACATATATATATATATATATATATATATATATATATATATATATATATATATATTTTTTTTCAAAAAAAAAAAAAATATATAATAATAATAAGTTTATATATAGTTTTAAATTTATCAAGAAGGTATAAATGCGAGACTTTGAAAATTATGATGTACAGTATAATTGTACCTTATATGTGTGTATAAATTATTTGAAATTTTTATATGTTTTGTGGATCATTTTAATATTTATTAAATTGTTCTTTAGTGAAAACAAATTGACAAAGCTCGTTAGTAAAAATTGTTAAAACAACTTTATATGTAATAAAGTATATAATATGTCTTGCTCAAAAAAAGTTTATAACATGTCAAGCATAATATATATATATATATATATATATATATATATATATATATATATATATATGTATATGTGTGTGTGTGTGTGTGTGTGTGTGTAATTACCCCTTTTTATTGAGAACTATTATTTGTATAACCATTTTTTTACAACAACTTTTTTCTCATATTCACATTATGTTTTTATTATTAATTTCTCTCTATAATTTTAGTTTTTGTGTCAATACCTTTTTTCTTTGTAAATTTATGGTTGTCTCATAAGTTGTCATAGAAATGATTGTTCAAATAACACAACTCTTTATGTAAGCTTGCATCATCATCATGTGAAAAAGAAACATAATAATAATAATAATATTAATAGTAATAAAATTCAACATGGAGAAAATCTTTATTATATTTAAAATTAAATAATTAACTTTGGATACACTCACATGCATAGATCATACTTGTAAGTTTTACATGGATCCAAAAATATTTTATATAGACCACCTCTCTAAATCTTTACATAAATCTAAAATGATTTGTTGTGTTATCAACATGCATCAATATTGATATGTTTAATAAAAGAACTTAAAATTTTGATCTTGAACAAAAATATATAATATACTAAATGGTTTGAAATTTACTTAAACTTTGAACAGACGATATCTCTCTCACTGCGTGTGCATATACATGTGTGTGTGATATAGCGAGAATTTTTAATCAAATAGCAAAAAATTTAAAATACTTCTGAAAAAGTTATTGAGTATTGCAACACTATCCAAATGTTACATTAGTCTAATTTGATATTTTCAATCAGTTTGGATTGAAAGGAGTAAATCATGAATGTATAAGGAAAATGTTTAATGTAGTACACCTTGAGACAGAAAGAGATGAGAGAGTGATGTGATAAATATGTTATATCGATGATCTAATATAAAGATATGGTGTTCAAATGAGTGTAAAGTAATATCAGGTGTTTAAATATCATAGTTGAATTGTGTAAAAAAAAATACCATAGTTGAATATATGGTAATCAAACTGTTAGAATTAAAAGTATAATAATCACATGAGTACCACTAAAAAAGATATGACGTTATATTCTAATCTTAACTAATCATTCATGATTTGATTCCCTTTGTTTAATATTAAGAACCTTATGATAATTTAATATAACATTAATCTTCTTTGTGATTATGACAATATTTTAGGAGGAGCTTGTGGTTATGATGATATCTTTCATGCTGGGTTTGGAGTGAACACAGCAGCAGTGAGCACTATGCTTTTCAGAGATGGTGAGGTTTGTGGTGCTTGCTACCAAGTCATATGTGACTATAAAATCGACCCGAAATGGTGTCTTAGAAGTCGAAGTGTTACAGTAACTGCCACAAATTTTTGTCCTCCAAACAATCATGGAGGATGGTGTGATCCTCCACACCATCACTTTGATATGTCCATGCCTGCTTTCTTTCGCATTGCACGACAAGGCAATGAAGGCGTTGTTCCTGTTCTTTATAGAAGGTAGAATAAAACATATCTGTCTTTGTCTTTGTCATCTCTCTTTAATGGGCAATGATGGTGCATTCTTTGTTACAAATTTGAAACTAAATAGTTACTTTTTCAAATGCATAGAACATCTTGAAAATTTTATACAAATCTAGATATTTTCGTAGAGCTGTAAATTTTACATGAATCTTAAATTTTTGATTGTATAGGATGACAATATTGACATTGTTTAATAAAAAACTAACAACCGAAAAATAAATTTCTACCTCCAAGCTAAGTAACCAGTGTCCTCGAGACATTAGTTAAGAAAACAAGAAATAAAAAATTTGCATTAAAAATATCATTTTTCATACTTTTAAAAATTTGACTACACACTTTTTAAGAAAGTTTTACCATATTTAATTCTTTGACTAATACTTAAGGACACTAGTTATCATTTTCTTTTTTTATAAATAAAAAATCTAATGAGGTTACATGTTTTTATTGAAGACACTATGAAAGTGGTGTGAATAAGCATCTCATTTCATACCCAATATCTTAATGACAAACAAATAATTAGCATTAAGAAAAGTGCATGTATAAAATAATCAAGAAACATCTCCCTTTATATATGATACATGATGAGGGAATTTCATAATTTTTTCCGATCTCTGTAAGAGCAGTAAACTCTTACTACTAGTTGGTTTTTTATATATATATATTTTACGAATGCATATAAATTATAATATGGACCTCAAAAAGAGATTTATAGTTTTATTGTAAAAAAAAAAAGAAGAATATTTATGGGTGGACTTTACTATTATAGTAAATTTTAATAAAATATTACATCTCTTTTTCTTTCTTTTTTATTGTAAAATCCACTATTTCACTAATGGTTATCACAAAAGCTCCTGCTATAGGGATCCACACACGTAAACTTTTTCGTTAGTCCATAATTATAATGTAGGCCTCACCGTATTATATATACATGTACTTGAACAGGGTAGCATGCAAAAGAAGAGGAGGAGTTAGGTTCACATTGAAGGGTCAATCAAATTTCAACATGGTGATGATAACAAACGTGGGTGGTAGCGGTGATGTGAAGGGAGTGTGGATAAGAGGTTCCAGAAGTGGAGCTTGGTTGCCAATGCACAGAAATTGGGGAGCTAATTGGCAAAGCAGTGCAGATCTAAGGAACCAGAGACTCTCATTCAAGATTACTTTGGTTGATGGTAAAACACTGGTGTTTCTCAATGTAGTTTCTTCCAATTGGAGATTTGGACAAACATTTTCATCCCATAATCAATTCTATTAG
Protein sequence

>MS.gene34210.t1

MGPLMISSFNFIWCLLLVLCAVRIHVEANGWLNAHATFYGANQSPTSLGGACGYDDIFHAGFGVNTAAVSTMLFRDGEVCGACYQVICDYKIDPKWCLRSRSVTVTATNFCPPNNHGGWCDPPHHHFDMSMPAFFRIARQGNEGVVPVLYRRVACKRRGGVRFTLKGQSNFNMVMITNVGGSGDVKGVWIRGSRSGAWLPMHRNWGANWQSSADLRNQRLSFKITLVDGKTLVFLNVVSSNWRFGQTFSSHNQFY