AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene34524

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34524.t1 MTR_1g023070 93.333 120 8 0 1 120 1 120 1.87e-72 219
MS.gene34524.t1 MTR_1g023070 93.333 120 8 0 1 120 1 120 3.89e-72 218
MS.gene34524.t1 MTR_1g009617 89.474 95 10 0 26 120 167 261 1.39e-55 182
MS.gene34524.t1 MTR_5g069330 70.588 119 34 1 1 119 248 365 4.42e-53 176
MS.gene34524.t1 MTR_5g069330 70.588 119 34 1 1 119 244 361 4.74e-53 176
MS.gene34524.t1 MTR_6g082080 59.836 122 47 1 1 120 248 369 2.19e-41 146
MS.gene34524.t1 MTR_7g056830 59.836 122 47 1 1 120 248 369 2.21e-41 146
MS.gene34524.t1 MTR_4g098870 92.000 75 6 0 125 199 305 379 1.06e-40 146
MS.gene34524.t1 MTR_4g098870 92.000 75 6 0 125 199 285 359 1.08e-40 146
MS.gene34524.t1 MTR_4g098870 92.000 75 6 0 125 199 285 359 1.11e-40 146
MS.gene34524.t1 MTR_4g098870 92.000 75 6 0 125 199 305 379 1.19e-40 146
MS.gene34524.t1 MTR_4g098870 92.000 75 6 0 125 199 324 398 1.44e-40 146
MS.gene34524.t1 MTR_5g014040 75.342 73 18 0 130 202 251 323 1.91e-29 115
MS.gene34524.t1 MTR_5g014040 75.342 73 18 0 130 202 291 363 2.43e-29 115
MS.gene34524.t1 MTR_1g022305 64.286 84 29 1 37 120 221 303 1.07e-28 112
MS.gene34524.t1 MTR_5g014040 78.571 70 15 0 130 199 291 360 1.37e-28 112
MS.gene34524.t1 MTR_2g066050 35.385 130 78 2 5 128 298 427 1.86e-20 89.7
MS.gene34524.t1 MTR_2g090675 54.930 71 27 1 134 199 140 210 9.21e-20 87.4
MS.gene34524.t1 MTR_4g078810 66.038 53 18 0 147 199 155 207 1.19e-19 87.0
MS.gene34524.t1 MTR_2g450070 59.259 54 22 0 146 199 209 262 3.62e-15 74.3
MS.gene34524.t1 MTR_3g106220 52.113 71 33 1 139 208 187 257 3.69e-15 74.3
MS.gene34524.t1 MTR_2g450070 59.259 54 22 0 146 199 209 262 3.72e-15 74.3
MS.gene34524.t1 MTR_4g121020 54.930 71 29 2 149 216 110 180 9.85e-15 73.2
MS.gene34524.t1 MTR_4g121020 54.930 71 29 2 149 216 214 284 1.09e-14 73.2
MS.gene34524.t1 MTR_4g121020 54.930 71 29 2 149 216 214 284 1.13e-14 73.2
MS.gene34524.t1 MTR_4g131570 60.000 55 22 0 145 199 198 252 1.27e-14 72.8
MS.gene34524.t1 MTR_1g032570 57.377 61 25 1 149 208 130 190 2.39e-14 72.0
MS.gene34524.t1 MTR_1g032570 57.377 61 25 1 149 208 199 259 2.73e-14 72.0
MS.gene34524.t1 MTR_4g131600 55.000 60 24 1 140 199 194 250 1.46e-13 69.7
MS.gene34524.t1 MTR_5g027840 39.437 71 43 0 22 92 371 441 4.54e-13 68.2
MS.gene34524.t1 MTR_7g089010 44.872 78 38 1 122 199 68 140 5.10e-13 67.4
MS.gene34524.t1 MTR_4g064730 58.824 51 21 0 149 199 130 180 1.12e-12 66.2
MS.gene34524.t1 MTR_7g076820 29.412 119 82 1 6 122 389 507 1.23e-12 67.0
MS.gene34524.t1 MTR_4g131580 59.259 54 22 0 146 199 196 249 1.34e-12 67.0
MS.gene34524.t1 MTR_7g076800 28.571 119 83 1 6 122 380 498 1.97e-12 66.2
MS.gene34524.t1 MTR_7g076830 28.571 119 83 1 6 122 382 500 2.03e-12 66.2
MS.gene34524.t1 MTR_1g013170 51.613 62 29 1 148 208 189 250 2.16e-12 66.2
MS.gene34524.t1 MTR_8g079940 56.452 62 26 1 149 209 208 269 2.25e-12 66.2
MS.gene34524.t1 MTR_3g102590 52.542 59 27 1 143 201 137 194 2.47e-12 63.9
MS.gene34524.t1 MTR_2g039960 33.735 83 53 1 46 126 322 404 3.36e-12 65.5
MS.gene34524.t1 MTR_2g034960 44.156 77 37 2 149 219 210 286 3.82e-12 65.5
MS.gene34524.t1 MTR_1g013180 55.769 52 23 0 148 199 340 391 3.92e-12 65.5
MS.gene34524.t1 MTR_3g071620 30.172 116 75 2 7 116 280 395 4.93e-12 65.1
MS.gene34524.t1 MTR_3g102590 49.275 69 31 2 143 208 137 204 5.48e-12 63.5
MS.gene34524.t1 MTR_7g117705 51.613 62 29 1 148 208 189 250 6.19e-12 64.7
MS.gene34524.t1 MTR_3g086100 50.000 66 32 1 146 210 124 189 6.86e-12 64.7
MS.gene34524.t1 MTR_3g086100 50.000 66 32 1 146 210 195 260 6.96e-12 64.7
MS.gene34524.t1 MTR_7g026400 50.000 62 30 1 149 209 156 217 8.01e-12 64.7
MS.gene34524.t1 MTR_4g021760 31.132 106 63 1 104 199 113 218 8.25e-12 63.9
MS.gene34524.t1 MTR_3g107420 34.314 102 65 1 7 106 292 393 9.05e-12 64.3
MS.gene34524.t1 MTR_1g012420 34.314 102 65 1 7 106 292 393 1.03e-11 63.9
MS.gene34524.t1 MTR_4g021790 46.970 66 35 0 134 199 152 217 1.04e-11 63.2
MS.gene34524.t1 MTR_4g010565 34.314 102 65 1 7 106 369 470 1.09e-11 63.9
MS.gene34524.t1 MTR_3g102600 53.333 45 21 0 155 199 88 132 1.40e-11 60.8
MS.gene34524.t1 MTR_2g055850 31.132 106 70 1 7 109 341 446 1.67e-11 63.5
MS.gene34524.t1 MTR_3g102600 53.333 45 21 0 155 199 137 181 2.75e-11 60.8
MS.gene34524.t1 MTR_4g021845 50.000 52 26 0 149 200 167 218 3.27e-11 61.2
MS.gene34524.t1 MTR_3g102600 49.020 51 26 0 149 199 82 132 3.54e-11 60.5
MS.gene34524.t1 MTR_3g102600 49.020 51 26 0 149 199 131 181 5.37e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34524.t1 AT5G60990 54.098 122 54 1 1 120 258 379 1.99e-37 136
MS.gene34524.t1 AT1G16280 37.288 118 72 1 5 120 304 421 6.14e-21 91.3
MS.gene34524.t1 AT4G18020 78.182 55 10 1 145 199 293 345 3.49e-20 89.0
MS.gene34524.t1 AT4G18020 78.182 55 10 1 145 199 293 345 3.49e-20 89.0
MS.gene34524.t1 AT4G18020 78.182 55 10 1 145 199 293 345 3.49e-20 89.0
MS.gene34524.t1 AT4G18020 78.182 55 10 1 145 199 293 345 3.49e-20 89.0
MS.gene34524.t1 AT4G18020 78.182 55 10 1 145 199 293 345 3.49e-20 89.0
MS.gene34524.t1 AT4G18020 78.182 55 10 1 145 199 293 345 3.49e-20 89.0
MS.gene34524.t1 AT4G18020 78.182 55 10 1 145 199 293 345 3.59e-20 89.0
MS.gene34524.t1 AT4G18020 78.182 55 10 1 145 199 293 345 3.59e-20 89.0
MS.gene34524.t1 AT4G18020 78.182 55 10 1 145 199 293 345 3.59e-20 89.0
MS.gene34524.t1 AT4G18020 78.182 55 10 1 145 199 293 345 3.59e-20 89.0
MS.gene34524.t1 AT4G18020 78.182 55 10 1 145 199 293 345 3.59e-20 89.0
MS.gene34524.t1 AT5G44190 73.077 52 14 0 148 199 145 196 6.42e-20 87.4
MS.gene34524.t1 AT5G44190 73.077 52 14 0 148 199 145 196 7.03e-20 87.4
MS.gene34524.t1 AT2G20570 71.698 53 15 0 147 199 150 202 1.20e-19 87.0
MS.gene34524.t1 AT2G20570 75.510 49 12 0 151 199 170 218 1.52e-18 84.0
MS.gene34524.t1 AT5G58080 62.963 54 20 0 146 199 190 243 1.69e-16 78.6
MS.gene34524.t1 AT1G31970 37.363 91 55 1 19 107 382 472 5.91e-15 73.9
MS.gene34524.t1 AT2G01760 56.250 64 27 1 146 208 131 194 7.36e-15 72.8
MS.gene34524.t1 AT2G01760 56.250 64 27 1 146 208 224 287 1.22e-14 72.8
MS.gene34524.t1 AT3G16857 56.923 65 27 1 141 204 158 222 1.24e-14 72.8
MS.gene34524.t1 AT2G01760 56.250 64 27 1 146 208 196 259 1.27e-14 72.4
MS.gene34524.t1 AT3G16857 48.810 84 37 2 141 218 228 311 1.47e-14 72.8
MS.gene34524.t1 AT3G16857 48.810 84 37 2 141 218 228 311 1.51e-14 72.8
MS.gene34524.t1 AT2G25180 59.677 62 24 1 148 208 193 254 2.20e-14 72.0
MS.gene34524.t1 AT2G40970 47.368 76 37 1 124 199 82 154 6.07e-14 69.3
MS.gene34524.t1 AT4G31920 42.353 85 48 1 138 221 171 255 6.34e-14 70.9
MS.gene34524.t1 AT5G05090 58.824 51 21 0 149 199 80 130 1.99e-13 68.2
MS.gene34524.t1 AT5G59570 47.826 69 36 0 131 199 122 190 2.10e-13 68.6
MS.gene34524.t1 AT5G59570 47.826 69 36 0 131 199 122 190 2.10e-13 68.6
MS.gene34524.t1 AT3G10760 58.824 51 21 0 149 199 104 154 2.44e-13 68.6
MS.gene34524.t1 AT3G46640 58.824 51 21 0 149 199 143 193 8.67e-13 67.0
MS.gene34524.t1 AT3G46640 58.824 51 21 0 149 199 143 193 8.67e-13 67.0
MS.gene34524.t1 AT3G46640 58.824 51 21 0 149 199 143 193 8.80e-13 67.0
MS.gene34524.t1 AT3G22310 30.952 126 84 2 3 126 361 485 9.54e-13 67.4
MS.gene34524.t1 AT3G22310 30.952 126 84 2 3 126 361 485 9.62e-13 67.4
MS.gene34524.t1 AT5G49240 55.769 52 23 0 149 200 222 273 9.84e-13 66.6
MS.gene34524.t1 AT4G00660 28.571 119 83 1 6 122 371 489 2.64e-12 65.9
MS.gene34524.t1 AT4G00660 28.571 119 83 1 6 122 371 489 2.64e-12 65.9
MS.gene34524.t1 AT3G61240 28.571 119 83 1 6 122 364 482 3.17e-12 65.9
MS.gene34524.t1 AT3G61240 28.571 119 83 1 6 122 364 482 3.17e-12 65.9
MS.gene34524.t1 AT2G45810 28.571 119 83 1 6 122 394 512 7.08e-12 64.7

Find 48 sgRNAs with CRISPR-Local

Find 177 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTCAGAATCTTGGTTTC+AGG 0.140899 6.3:+54490834 MS.gene34524:CDS
CACTATCCCTTCTCGAATAT+TGG 0.236667 6.3:+54493490 MS.gene34524:CDS
TGACTCATATAACTAATAAT+AGG 0.255686 6.3:-54490863 None:intergenic
GCTTTGATTCTTCAGAATCT+TGG 0.269332 6.3:+54490827 MS.gene34524:CDS
TCCTTGAATGCGTTCAAGTC+TGG 0.307276 6.3:+54490996 MS.gene34524:CDS
TTGAAATGTCTCCTTTCATC+TGG 0.335756 6.3:-54491256 None:intergenic
ATGTGGTACAACGCTTCTTT+TGG 0.360475 6.3:+54490805 MS.gene34524:CDS
TAATGTGGCAAGCCATCTTC+AGG 0.386166 6.3:+54493544 MS.gene34524:CDS
TTGTAGTGACTTTGTTAGTA+AGG 0.398889 6.3:+54491037 MS.gene34524:CDS
CCCAGACTTGAACGCATTCA+AGG 0.403087 6.3:-54490997 None:intergenic
GACTCATATAACTAATAATA+GGG 0.421629 6.3:-54490862 None:intergenic
ATCTGGGCTCACAAAGGAAA+TGG 0.429905 6.3:-54491239 None:intergenic
TCTTCAGAATCTTGGTTTCA+GGG 0.431999 6.3:+54490835 MS.gene34524:CDS
TATTATTAGTTATATGAGTC+AGG 0.445349 6.3:+54490865 MS.gene34524:CDS
AGATATTCCAGCGGTAGATA+TGG 0.449221 6.3:+54491064 MS.gene34524:CDS
TACTTTGGTCTTAGGTAGAC+TGG 0.457759 6.3:+54493411 MS.gene34524:intron
AATGTGAGATAAAAGCTAAT+CGG 0.462021 6.3:+54493232 MS.gene34524:CDS
CCTTGAATGCGTTCAAGTCT+GGG 0.463606 6.3:+54490997 MS.gene34524:CDS
AATATGATAGAGAAACATAT+AGG 0.463712 6.3:+54491277 MS.gene34524:CDS
TGATTAACTACAACATTCCT+TGG 0.469598 6.3:+54491087 MS.gene34524:CDS
CCTTTGTGAGCCCAGATGAA+AGG 0.506008 6.3:+54491245 MS.gene34524:CDS
TACACTCTCACTAAAATGAC+TGG 0.518270 6.3:+54490755 None:intergenic
AATATTGGATCTAATGAAAG+TGG 0.519493 6.3:+54493505 MS.gene34524:CDS
TGTAGTGACTTTGTTAGTAA+GGG 0.522265 6.3:+54491038 MS.gene34524:CDS
ATTAGATCCAATATTCGAGA+AGG 0.526425 6.3:-54493497 None:intergenic
GTGAAGGCAGTGGAGCAACT+AGG 0.527044 6.3:+54493458 MS.gene34524:CDS
ACAACATCTGCAAATAAAGT+AGG 0.528162 6.3:+54493195 MS.gene34524:CDS
GCAAATAAAGTAGGTATTCG+CGG 0.536109 6.3:+54493204 MS.gene34524:CDS
TGAAATGTCTCCTTTCATCT+GGG 0.550468 6.3:-54491255 None:intergenic
AATGACTGGAAGTACATCAA+TGG 0.550477 6.3:+54490769 None:intergenic
ATTACATCTACCGCGTGGGA+TGG 0.552861 6.3:+54491194 MS.gene34524:CDS
GGTAGATTATAACCTGAAGA+TGG 0.564115 6.3:-54493556 None:intergenic
TTAATCACCATATCTACCGC+TGG 0.579180 6.3:-54491071 None:intergenic
GGTTATAATCTACCTTCCCA+CGG 0.590624 6.3:+54493565 MS.gene34524:CDS
TTAGATCCAATATTCGAGAA+GGG 0.597968 6.3:-54493496 None:intergenic
CATATAACTAATAATAGGGA+TGG 0.602559 6.3:-54490858 None:intergenic
ATTGGATCTAATGAAAGTGG+AGG 0.613074 6.3:+54493508 MS.gene34524:CDS
CATCTTACGTTTGGATACCA+AGG 0.615406 6.3:-54491104 None:intergenic
GGGCTTGACAAGGCATAATG+TGG 0.631155 6.3:+54493529 MS.gene34524:CDS
TTGGATCTAATGAAAGTGGA+GGG 0.637148 6.3:+54493509 MS.gene34524:CDS
CCTTTCATCTGGGCTCACAA+AGG 0.638109 6.3:-54491245 None:intergenic
CTTGAATGCGTTCAAGTCTG+GGG 0.643789 6.3:+54490998 MS.gene34524:CDS
TGAAAGTGGAGGGCTTGACA+AGG 0.644049 6.3:+54493519 MS.gene34524:CDS
CAGGATTACATCTACCGCGT+GGG 0.645040 6.3:+54491190 MS.gene34524:intron
ACAGGATTACATCTACCGCG+TGG 0.703821 6.3:+54491189 MS.gene34524:intron
ATGGTATACACTCAGCAATG+TGG 0.716399 6.3:+54490788 MS.gene34524:CDS
TAAGGGACTAGATATTCCAG+CGG 0.751272 6.3:+54491055 MS.gene34524:CDS
GAGCAACATTCCATCCCACG+CGG 0.794839 6.3:-54491204 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAATTAATGTAGTTTAAA+AGG - chr6.3:54492781-54492800 None:intergenic 10.0%
!! TATGTATAAATGAATTATTA+AGG - chr6.3:54492414-54492433 None:intergenic 10.0%
!!! TATAAAATACTAATTTCTTT+TGG - chr6.3:54491755-54491774 None:intergenic 10.0%
!!! TTAACATAATAACTTTTTAT+AGG + chr6.3:54490959-54490978 MS.gene34524:intron 10.0%
!! AAGTTATTATGTTAAGAATT+AGG - chr6.3:54490954-54490973 None:intergenic 15.0%
!! AATAAAATTCTAATTGTCAA+TGG - chr6.3:54491861-54491880 None:intergenic 15.0%
!! AGAAATTACAATTATAATAC+AGG - chr6.3:54490907-54490926 None:intergenic 15.0%
!! ATAAAATTCTAATTGTCAAT+GGG - chr6.3:54491860-54491879 None:intergenic 15.0%
!! GTAAGAAATAAATACTATTA+TGG - chr6.3:54492441-54492460 None:intergenic 15.0%
!! TATAAATGAATTATTAAGGA+CGG - chr6.3:54492410-54492429 None:intergenic 15.0%
!!! AATAAAAAGGTTTTACATAA+AGG + chr6.3:54492561-54492580 MS.gene34524:intron 15.0%
!!! ATAAAAAGGTTTTACATAAA+GGG + chr6.3:54492562-54492581 MS.gene34524:intron 15.0%
!!! ATTTTTACATTTGTTTCATA+TGG + chr6.3:54492066-54492085 MS.gene34524:intron 15.0%
!!! TAATATGACAGTTTTTTTTA+GGG + chr6.3:54491829-54491848 MS.gene34524:intron 15.0%
!!! TGATATTCATATATTTTCAT+AGG + chr6.3:54493639-54493658 MS.gene34524:CDS 15.0%
!!! TTTTTCAAGATCTTTTATTT+AGG + chr6.3:54492595-54492614 MS.gene34524:intron 15.0%
!! AAAACTGTATTAAGAAAATC+AGG - chr6.3:54493389-54493408 None:intergenic 20.0%
!! AATATGATAGAGAAACATAT+AGG + chr6.3:54491277-54491296 MS.gene34524:CDS 20.0%
!! GACTCATATAACTAATAATA+GGG - chr6.3:54490865-54490884 None:intergenic 20.0%
!! GTTGAATTATTATAGTGTTA+TGG + chr6.3:54492276-54492295 MS.gene34524:intron 20.0%
!! TATTATTAGTTATATGAGTC+AGG + chr6.3:54490865-54490884 MS.gene34524:CDS 20.0%
!! TCCTTTCTATATCTAATTAT+CGG - chr6.3:54491630-54491649 None:intergenic 20.0%
!! TGACTCATATAACTAATAAT+AGG - chr6.3:54490866-54490885 None:intergenic 20.0%
!! TTGTTTATCAATGTTGTATA+AGG + chr6.3:54493329-54493348 MS.gene34524:intron 20.0%
!!! AAATAGAGAAGTTAAGTTTT+TGG + chr6.3:54491545-54491564 MS.gene34524:intron 20.0%
!!! AACTACTCTAATGTTTATTT+TGG + chr6.3:54493050-54493069 MS.gene34524:intron 20.0%
!!! ACTACTCTAATGTTTATTTT+GGG + chr6.3:54493051-54493070 MS.gene34524:intron 20.0%
!!! ATCTTTTATTTAGGAATTGA+TGG + chr6.3:54492604-54492623 MS.gene34524:intron 20.0%
!!! ATTAATTACAGTTTTGAATG+AGG - chr6.3:54493612-54493631 None:intergenic 20.0%
!!! ATTATATCTAGTGTCAATTT+TGG - chr6.3:54492500-54492519 None:intergenic 20.0%
!!! GTAATATGACAGTTTTTTTT+AGG + chr6.3:54491828-54491847 MS.gene34524:intron 20.0%
!!! TGATTTTCTTAATACAGTTT+TGG + chr6.3:54493388-54493407 MS.gene34524:intron 20.0%
! AAAAAAGTAGTAACTGTTAG+AGG - chr6.3:54493087-54493106 None:intergenic 25.0%
! AACATTATGAGAAGAATGAA+TGG - chr6.3:54493283-54493302 None:intergenic 25.0%
! AATATTGGATCTAATGAAAG+TGG + chr6.3:54493505-54493524 MS.gene34524:CDS 25.0%
! AATGTGAGATAAAAGCTAAT+CGG + chr6.3:54493232-54493251 MS.gene34524:CDS 25.0%
! AATTCACCTATGAATATGAA+GGG + chr6.3:54491905-54491924 MS.gene34524:intron 25.0%
! AGGGATAAATGTGAATAAAA+AGG + chr6.3:54492548-54492567 MS.gene34524:intron 25.0%
! ATGCACAAATATATAGACAT+TGG + chr6.3:54492135-54492154 MS.gene34524:intron 25.0%
! CATATAACTAATAATAGGGA+TGG - chr6.3:54490861-54490880 None:intergenic 25.0%
! TACATTTGTTTCATATGGAT+TGG + chr6.3:54492071-54492090 MS.gene34524:intron 25.0%
! TCATTAAAATGGCAAAGTAA+AGG - chr6.3:54491312-54491331 None:intergenic 25.0%
! TCCGATAATTAGATATAGAA+AGG + chr6.3:54491626-54491645 MS.gene34524:intron 25.0%
! TCTATTTAAAAGAAGTAGAC+AGG - chr6.3:54491532-54491551 None:intergenic 25.0%
! TTATACCAATGAAGACAAAT+TGG - chr6.3:54491598-54491617 None:intergenic 25.0%
! TTGGAAACACCATTATATTA+TGG - chr6.3:54492748-54492767 None:intergenic 25.0%
!! AAAGCGTCATAGTAAATAAT+TGG + chr6.3:54491335-54491354 MS.gene34524:intron 25.0%
!! TGACTTATTAAATTTTCAGC+AGG - chr6.3:54491990-54492009 None:intergenic 25.0%
!! TTTAAAAGGTAGTCTTCTTT+TGG - chr6.3:54492767-54492786 None:intergenic 25.0%
!! TTTTTATAGGCAAAGAAACT+TGG + chr6.3:54490972-54490991 MS.gene34524:intron 25.0%
!! TTTTTCTAGATCTTGAAGTT+TGG - chr6.3:54492109-54492128 None:intergenic 25.0%
!!! ACTACTTTTTTTTCTTCTTC+TGG + chr6.3:54493096-54493115 MS.gene34524:intron 25.0%
!!! AGTTAAGTTTTTGGATTTTG+TGG + chr6.3:54491554-54491573 MS.gene34524:intron 25.0%
!!! GTGTTACTTTTTTGTTCAAT+TGG + chr6.3:54492254-54492273 MS.gene34524:intron 25.0%
!!! TGAGGTATTGAAATAGTTTT+TGG - chr6.3:54493594-54493613 None:intergenic 25.0%
!!! TTAATACAGTTTTGGTACTT+TGG + chr6.3:54493396-54493415 MS.gene34524:intron 25.0%
AAAAGCAAAACTTCCTCAAA+TGG - chr6.3:54491420-54491439 None:intergenic 30.0%
ACAACATCTGCAAATAAAGT+AGG + chr6.3:54493195-54493214 MS.gene34524:CDS 30.0%
AGAACCTGAGAGATATTATT+AGG + chr6.3:54492528-54492547 MS.gene34524:intron 30.0%
AGTATAGTTGTATCAACACT+TGG + chr6.3:54492942-54492961 MS.gene34524:intron 30.0%
ATGGATATTGAAAATGTGTG+AGG - chr6.3:54492191-54492210 None:intergenic 30.0%
ATTAGATCCAATATTCGAGA+AGG - chr6.3:54493500-54493519 None:intergenic 30.0%
ATTGAGCATAGTTATGCTTT+AGG + chr6.3:54491492-54491511 MS.gene34524:intron 30.0%
CAATGAACAGTGTGAAATAA+CGG - chr6.3:54493312-54493331 None:intergenic 30.0%
CAATTCACCTATGAATATGA+AGG + chr6.3:54491904-54491923 MS.gene34524:intron 30.0%
GAACCTGAGAGATATTATTA+GGG + chr6.3:54492529-54492548 MS.gene34524:intron 30.0%
GAATATGGAGTTTGTATCTT+AGG + chr6.3:54492373-54492392 MS.gene34524:intron 30.0%
GAATTAGGCATATTTCTTGT+TGG - chr6.3:54490939-54490958 None:intergenic 30.0%
GATAGAGAAACATATAGGTT+AGG + chr6.3:54491282-54491301 MS.gene34524:intron 30.0%
GCAAGGCATATAATTGAAAA+AGG - chr6.3:54492648-54492667 None:intergenic 30.0%
GTTCTCTCACCATAATATAA+TGG + chr6.3:54492736-54492755 MS.gene34524:intron 30.0%
TATATAGCACATCTTACGTT+TGG - chr6.3:54491116-54491135 None:intergenic 30.0%
TATCCCTAATAATATCTCTC+AGG - chr6.3:54492535-54492554 None:intergenic 30.0%
TCATATTCATAGGTGAATTG+TGG - chr6.3:54491904-54491923 None:intergenic 30.0%
TGATTAACTACAACATTCCT+TGG + chr6.3:54491087-54491106 MS.gene34524:CDS 30.0%
TGTAGTGACTTTGTTAGTAA+GGG + chr6.3:54491038-54491057 MS.gene34524:CDS 30.0%
TTAGATCCAATATTCGAGAA+GGG - chr6.3:54493499-54493518 None:intergenic 30.0%
TTGGGAAAAAAAAGAACAAG+AGG + chr6.3:54492702-54492721 MS.gene34524:intron 30.0%
TTGTAGTGACTTTGTTAGTA+AGG + chr6.3:54491037-54491056 MS.gene34524:CDS 30.0%
! ATATCTAGTGTCAATTTTGG+TGG - chr6.3:54492497-54492516 None:intergenic 30.0%
! ATTTGAGGAAGTTTTGCTTT+TGG + chr6.3:54491419-54491438 MS.gene34524:intron 30.0%
! GACGCTTTTGTTCATTAAAA+TGG - chr6.3:54491323-54491342 None:intergenic 30.0%
!! AAATAGTTTTTGGAAACCGT+GGG - chr6.3:54493584-54493603 None:intergenic 30.0%
!! TATCTAGTGTCAATTTTGGT+GGG - chr6.3:54492496-54492515 None:intergenic 30.0%
!! TTGTTTCATATGGATTGGAT+TGG + chr6.3:54492076-54492095 MS.gene34524:intron 30.0%
!! TTTTGGAAATTCACTTGAGA+GGG - chr6.3:54491738-54491757 None:intergenic 30.0%
AATGTATGATAAGACTGCAC+GGG + chr6.3:54491649-54491668 MS.gene34524:intron 35.0%
ACAAGCATATCAAAGAGACA+AGG - chr6.3:54492231-54492250 None:intergenic 35.0%
AGAATTGCATTACCGTTGAA+GGG - chr6.3:54492994-54493013 None:intergenic 35.0%
ATCATGCACATATATGAGCA+AGG + chr6.3:54492346-54492365 MS.gene34524:intron 35.0%
ATGTAATCCTGTTGATCACA+CGG - chr6.3:54491181-54491200 None:intergenic 35.0%
ATTCACTTGAGAGGGATATT+AGG - chr6.3:54491730-54491749 None:intergenic 35.0%
ATTGAAAATGTGTGAGGTGT+GGG - chr6.3:54492185-54492204 None:intergenic 35.0%
ATTGGATCTAATGAAAGTGG+AGG + chr6.3:54493508-54493527 MS.gene34524:CDS 35.0%
CTACAACCCTTCATATTCAT+AGG - chr6.3:54491914-54491933 None:intergenic 35.0%
GAATGTATGATAAGACTGCA+CGG + chr6.3:54491648-54491667 MS.gene34524:intron 35.0%
GCAAATAAAGTAGGTATTCG+CGG + chr6.3:54493204-54493223 MS.gene34524:CDS 35.0%
GCTTTGATTCTTCAGAATCT+TGG + chr6.3:54490827-54490846 MS.gene34524:CDS 35.0%
GGTAGATTATAACCTGAAGA+TGG - chr6.3:54493559-54493578 None:intergenic 35.0%
TATTGAAAATGTGTGAGGTG+TGG - chr6.3:54492186-54492205 None:intergenic 35.0%
TCATGCACATATATGAGCAA+GGG + chr6.3:54492347-54492366 MS.gene34524:intron 35.0%
TCTTCAGAATCTTGGTTTCA+GGG + chr6.3:54490835-54490854 MS.gene34524:CDS 35.0%
TGAAATGTCTCCTTTCATCT+GGG - chr6.3:54491258-54491277 None:intergenic 35.0%
TGGAAATCATTATAGCAGCA+TGG + chr6.3:54492296-54492315 MS.gene34524:intron 35.0%
TTCTTCAGAATCTTGGTTTC+AGG + chr6.3:54490834-54490853 MS.gene34524:CDS 35.0%
TTGAAATGTCTCCTTTCATC+TGG - chr6.3:54491259-54491278 None:intergenic 35.0%
TTGGATCTAATGAAAGTGGA+GGG + chr6.3:54493509-54493528 MS.gene34524:CDS 35.0%
TTTAATAAGTCACAGCCTCT+CGG + chr6.3:54491998-54492017 MS.gene34524:intron 35.0%
! AAAAAGTACAAGCTTGGAGT+TGG + chr6.3:54492898-54492917 MS.gene34524:intron 35.0%
! AAAAGTACAAGCTTGGAGTT+GGG + chr6.3:54492899-54492918 MS.gene34524:intron 35.0%
! ACAAAATTTTTGTGCAGCTC+TGG - chr6.3:54493440-54493459 None:intergenic 35.0%
! CAAAAATTTTGTGAAGGCAG+TGG + chr6.3:54493448-54493467 MS.gene34524:CDS 35.0%
! GCTGCACAAAAATTTTGTGA+AGG + chr6.3:54493442-54493461 MS.gene34524:CDS 35.0%
! TCTTTGAGCAAATGATAGTG+TGG - chr6.3:54491942-54491961 None:intergenic 35.0%
! TTTGTGGCAAGTTGAAATTG+TGG + chr6.3:54491570-54491589 MS.gene34524:intron 35.0%
!! AGGAAGTTTTGCTTTTGGAA+GGG + chr6.3:54491424-54491443 MS.gene34524:intron 35.0%
!! AGTTTTGGTACTTTGGTCTT+AGG + chr6.3:54493403-54493422 MS.gene34524:intron 35.0%
!! CATGTAGAGGTGATTTGTTT+GGG + chr6.3:54492684-54492703 MS.gene34524:intron 35.0%
!! CTTTTGGAAATTCACTTGAG+AGG - chr6.3:54491739-54491758 None:intergenic 35.0%
!! GAAATAGTTTTTGGAAACCG+TGG - chr6.3:54493585-54493604 None:intergenic 35.0%
!!! CTTGTACTTTTTTGCTGCTA+CGG - chr6.3:54492890-54492909 None:intergenic 35.0%
!!! TCAATTTTGGTGGGTTAGTT+CGG - chr6.3:54492487-54492506 None:intergenic 35.0%
AAAACTTCCTCAAATGGTGC+AGG - chr6.3:54491414-54491433 None:intergenic 40.0%
AACTTTGTGGATGCATGTAG+AGG + chr6.3:54492671-54492690 MS.gene34524:intron 40.0%
AGATATTCCAGCGGTAGATA+TGG + chr6.3:54491064-54491083 MS.gene34524:CDS 40.0%
ATGGTATACACTCAGCAATG+TGG + chr6.3:54490788-54490807 MS.gene34524:CDS 40.0%
ATTGTATCCTGCACCATTTG+AGG + chr6.3:54491404-54491423 MS.gene34524:intron 40.0%
CACTATCCCTTCTCGAATAT+TGG + chr6.3:54493490-54493509 MS.gene34524:CDS 40.0%
CATAGGTGAATTGTGGTGTT+GGG - chr6.3:54491897-54491916 None:intergenic 40.0%
CATCTTACGTTTGGATACCA+AGG - chr6.3:54491107-54491126 None:intergenic 40.0%
GAATTGCATTACCGTTGAAG+GGG - chr6.3:54492993-54493012 None:intergenic 40.0%
GAGAATTGCATTACCGTTGA+AGG - chr6.3:54492995-54493014 None:intergenic 40.0%
GGATCTGTGTGTATCATTGA+TGG - chr6.3:54492210-54492229 None:intergenic 40.0%
GGCTGAAAGATTATCACAAG+AGG + chr6.3:54491458-54491477 MS.gene34524:intron 40.0%
GGTTATAATCTACCTTCCCA+CGG + chr6.3:54493565-54493584 MS.gene34524:CDS 40.0%
TAAGGGACTAGATATTCCAG+CGG + chr6.3:54491055-54491074 MS.gene34524:CDS 40.0%
TATGCCTTGCTCAAACTTTG+TGG + chr6.3:54492658-54492677 MS.gene34524:intron 40.0%
TCAAATGGTGCAGGATACAA+TGG - chr6.3:54491405-54491424 None:intergenic 40.0%
TCATAGGTGAATTGTGGTGT+TGG - chr6.3:54491898-54491917 None:intergenic 40.0%
TTAATCACCATATCTACCGC+TGG - chr6.3:54491074-54491093 None:intergenic 40.0%
! ATGTGGTACAACGCTTCTTT+TGG + chr6.3:54490805-54490824 MS.gene34524:CDS 40.0%
! GCAGCAAAAAAGTACAAGCT+TGG + chr6.3:54492892-54492911 MS.gene34524:intron 40.0%
! TGGTGCCAATTTGTCTTCAT+TGG + chr6.3:54491590-54491609 MS.gene34524:intron 40.0%
!! GAGGAAGTTTTGCTTTTGGA+AGG + chr6.3:54491423-54491442 MS.gene34524:intron 40.0%
!! GCATGTAGAGGTGATTTGTT+TGG + chr6.3:54492683-54492702 MS.gene34524:intron 40.0%
!! TACTTTGGTCTTAGGTAGAC+TGG + chr6.3:54493411-54493430 MS.gene34524:intron 40.0%
AACTACCTACTAGCTAGCTG+CGG + chr6.3:54492967-54492986 MS.gene34524:intron 45.0%
AATGATGACTGTTTGCCGAG+AGG - chr6.3:54492016-54492035 None:intergenic 45.0%
ATCTGGGCTCACAAAGGAAA+TGG - chr6.3:54491242-54491261 None:intergenic 45.0%
CCTTGAATGCGTTCAAGTCT+GGG + chr6.3:54490997-54491016 MS.gene34524:CDS 45.0%
CTTGAATGCGTTCAAGTCTG+GGG + chr6.3:54490998-54491017 MS.gene34524:CDS 45.0%
GAGTCAGGTAACTGTACTAC+AGG + chr6.3:54490880-54490899 MS.gene34524:intron 45.0%
GCATCCACAAAGTTTGAGCA+AGG - chr6.3:54492665-54492684 None:intergenic 45.0%
TAATGTGGCAAGCCATCTTC+AGG + chr6.3:54493544-54493563 MS.gene34524:CDS 45.0%
TCCTTGAATGCGTTCAAGTC+TGG + chr6.3:54490996-54491015 MS.gene34524:CDS 45.0%
! TATGAGCAAGGGCGTGAATA+TGG + chr6.3:54492358-54492377 MS.gene34524:intron 45.0%
! TTTGGAAGGGTGTGCTATTG+AGG + chr6.3:54491437-54491456 MS.gene34524:intron 45.0%
!! AGTTTTTGGAAACCGTGGGA+AGG - chr6.3:54493580-54493599 None:intergenic 45.0%
!! AGAAATAATTAATAAATTAA+AGG + chr6.3:54492818-54492837 MS.gene34524:intron 5.0%
!!! ATTTATTTATTTATTTTTTC+AGG + chr6.3:54491369-54491388 MS.gene34524:intron 5.0%
ACAGGATTACATCTACCGCG+TGG + chr6.3:54491189-54491208 MS.gene34524:intron 50.0%
ATTACATCTACCGCGTGGGA+TGG + chr6.3:54491194-54491213 MS.gene34524:CDS 50.0%
CAGGATTACATCTACCGCGT+GGG + chr6.3:54491190-54491209 MS.gene34524:intron 50.0%
CCCAGACTTGAACGCATTCA+AGG - chr6.3:54491000-54491019 None:intergenic 50.0%
CCTTTCATCTGGGCTCACAA+AGG - chr6.3:54491248-54491267 None:intergenic 50.0%
CCTTTGTGAGCCCAGATGAA+AGG + chr6.3:54491245-54491264 MS.gene34524:CDS 50.0%
CTCATGACCGTGTGATCAAC+AGG + chr6.3:54491171-54491190 MS.gene34524:intron 50.0%
GGGCTTGACAAGGCATAATG+TGG + chr6.3:54493529-54493548 MS.gene34524:CDS 50.0%
TGAAAGTGGAGGGCTTGACA+AGG + chr6.3:54493519-54493538 MS.gene34524:CDS 50.0%
TGTGAGGTGTGGGAAGAAGA+GGG - chr6.3:54492175-54492194 None:intergenic 50.0%
GAGCAACATTCCATCCCACG+CGG - chr6.3:54491207-54491226 None:intergenic 55.0%
GGAGTTGGGACAACTGTCGT+TGG + chr6.3:54492913-54492932 MS.gene34524:intron 55.0%
GTGAAGGCAGTGGAGCAACT+AGG + chr6.3:54493458-54493477 MS.gene34524:CDS 55.0%
GTGTGAGGTGTGGGAAGAAG+AGG - chr6.3:54492176-54492195 None:intergenic 55.0%
GGGACAACTGTCGTTGGCAG+AGG + chr6.3:54492919-54492938 MS.gene34524:intron 60.0%
AGGGGCCGCAGCTAGCTAGT+AGG - chr6.3:54492975-54492994 None:intergenic 65.0%
GCTAGCTGCGGCCCCTTCAA+CGG + chr6.3:54492979-54492998 MS.gene34524:intron 65.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 54490770 54493664 54490770 ID=MS.gene34524
chr6.3 mRNA 54490770 54493664 54490770 ID=MS.gene34524.t1;Parent=MS.gene34524
chr6.3 exon 54490770 54490886 54490770 ID=MS.gene34524.t1.exon1;Parent=MS.gene34524.t1
chr6.3 CDS 54490770 54490886 54490770 ID=cds.MS.gene34524.t1;Parent=MS.gene34524.t1
chr6.3 exon 54490981 54491118 54490981 ID=MS.gene34524.t1.exon2;Parent=MS.gene34524.t1
chr6.3 CDS 54490981 54491118 54490981 ID=cds.MS.gene34524.t1;Parent=MS.gene34524.t1
chr6.3 exon 54491193 54491298 54491193 ID=MS.gene34524.t1.exon3;Parent=MS.gene34524.t1
chr6.3 CDS 54491193 54491298 54491193 ID=cds.MS.gene34524.t1;Parent=MS.gene34524.t1
chr6.3 exon 54493172 54493266 54493172 ID=MS.gene34524.t1.exon4;Parent=MS.gene34524.t1
chr6.3 CDS 54493172 54493266 54493172 ID=cds.MS.gene34524.t1;Parent=MS.gene34524.t1
chr6.3 exon 54493425 54493664 54493425 ID=MS.gene34524.t1.exon5;Parent=MS.gene34524.t1
chr6.3 CDS 54493425 54493664 54493425 ID=cds.MS.gene34524.t1;Parent=MS.gene34524.t1
Gene Sequence

>MS.gene34524

ATGACTGGAAGTACATCAATGGTATACACTCAGCAATGTGGTACAACGCTTCTTTTGGCTTTGATTCTTCAGAATCTTGGTTTCAGGGCCATCCCTATTATTAGTTATATGAGTCAGGTAACTGTACTACAGGTCCTGTATTATAATTGTAATTTCTTCTAAACTGCCAACAAGAAATATGCCTAATTCTTAACATAATAACTTTTTATAGGCAAAGAAACTTGGATCCTTGAATGCGTTCAAGTCTGGGGAGTGCAATATTCTCATTTGTAGTGACTTTGTTAGTAAGGGACTAGATATTCCAGCGGTAGATATGGTGATTAACTACAACATTCCTTGGTATCCAAACGTAAGATGTGCTATATAATGCTTTTATACTTTTGTCGATGTGTTTTAGTATACTCATGACCGTGTGATCAACAGGATTACATCTACCGCGTGGGATGGAATGTTGCTCATGTTAATGTTGCCATTTCCTTTGTGAGCCCAGATGAAAGGAGACATTTCAATATGATAGAGAAACATATAGGTTAGGAGAGCCTTTACTTTGCCATTTTAATGAACAAAAGCGTCATAGTAAATAATTGGTACAAGTATTTATTTATTTATTTATTTTTTCAGGCAAGAAACTACCATTGTATCCTGCACCATTTGAGGAAGTTTTGCTTTTGGAAGGGTGTGCTATTGAGGCTGAAAGATTATCACAAGAGGTAAATTTATGTATTGAGCATAGTTATGCTTTAGGCTACTTTCAGTTTTCCTGTCTACTTCTTTTAAATAGAGAAGTTAAGTTTTTGGATTTTGTGGCAAGTTGAAATTGTGGTGCCAATTTGTCTTCATTGGTATAAGCAATGTTTCCGATAATTAGATATAGAAAGGAATGTATGATAAGACTGCACGGGTCTATTAAAAATCACAAAATAACTAACAGAGATACAATTTTAAGATTAAAATCGTCCTAATATCCCTCTCAAGTGAATTTCCAAAAGAAATTAGTATTTTATATGATTAGCTTGTCTAAACAATATGAAAGTTCACTCGATCAAACTTATATAAAAGTAATATGACAGTTTTTTTTAGGGAAAAGCCCATTGACAATTAGAATTTTATTAATAATGTCTAAACCCAACACCACAATTCACCTATGAATATGAAGGGTTGTAGAAATTCCACACTATCATTTGCTCAAAGATCACATACAAAGTGTTCATCACTATCCTGCTGAAAATTTAATAAGTCACAGCCTCTCGGCAAACAGTCATCATTAGTTTATCAGTAGTGTGAGTATTAATATATATTTTTACATTTGTTTCATATGGATTGGATTGGTATGATTCCAAACTTCAAGATCTAGAAAAAGTAGTTATGCACAAATATATAGACATTGGAATAACTAATTGTTCCCTCTTCTTCCCACACCTCACACATTTTCAATATCCATCAATGATACACACAGATCCTTGTCTCTTTGATATGCTTGTATTGTGTTACTTTTTTGTTCAATTGGTTGAATTATTATAGTGTTATGGAAATCATTATAGCAGCATGGCATGTTACATATGTGTAACATAAATAGATCATGCACATATATGAGCAAGGGCGTGAATATGGAGTTTGTATCTTAGGTTATACTACATCCGTCCTTAATAATTCATTTATACATATTCACCATAATAGTATTTATTTCTTACTTGTTTTTTTTCTTTCTAATAAACCGAACTAACCCACCAAAATTGACACTAGATATAATCGAATCTGAGAACCTGAGAGATATTATTAGGGATAAATGTGAATAAAAAGGTTTTACATAAAGGGATAAGCTATATTTTTCAAGATCTTTTATTTAGGAATTGATGGAGTATATAGTTTCATAGACCTTTTTCAATTATATGCCTTGCTCAAACTTTGTGGATGCATGTAGAGGTGATTTGTTTGGGAAAAAAAAGAACAAGAGGATTAGAATTTCGTTCTCTCACCATAATATAATGGTGTTTCCAAAAGAAGACTACCTTTTAAACTACATTAATTATATAAGTATGAAGAATGAAAGAAATAATTAATAAATTAAAGGAATGAAACACTTTGAGTCTAAACTTGAACTGAGTGTTAGACTTAGACCGTAGCAGCAAAAAAGTACAAGCTTGGAGTTGGGACAACTGTCGTTGGCAGAGGAGTATAGTTGTATCAACACTTGGCAAACTACCTACTAGCTAGCTGCGGCCCCTTCAACGGTAATGCAATTCTCTGTATAGCTATGACTATGTGATGTAATTGTAATGCAACTACTCTAATGTTTATTTTGGGATATATTTGTCCTCTAACAGTTACTACTTTTTTTTCTTCTTCTGGATTTGAATTCAACATATGATAACTTTAATTTTATTCTTGTTTAAATTTTATAGTGACAATATTTAATGAAAGTAATACAACATCTGCAAATAAAGTAGGTATTCGCGGTCATAAATGTGAGATAAAAGCTAATCGGAAGAAGATGAAAGTAAGCTGTTTTTCCATTCATTCTTCTCATAATGTTCTTGTTCCGTTATTTCACACTGTTCATTGTTTATCAATGTTGTATAAGGTTATATTCATTTTATGTATGAGTGATCTACTGAACCTGATTTTCTTAATACAGTTTTGGTACTTTGGTCTTAGGTAGACTGGACGCCAGAGCTGCACAAAAATTTTGTGAAGGCAGTGGAGCAACTAGGCATTGATCACACTATCCCTTCTCGAATATTGGATCTAATGAAAGTGGAGGGCTTGACAAGGCATAATGTGGCAAGCCATCTTCAGGTTATAATCTACCTTCCCACGGTTTCCAAAAACTATTTCAATACCTCATTCAAAACTGTAATTAATGATAAATTGATATTCATATATTTTCATAGGTAA
Protein sequence

>MS.gene34524.t1

MTGSTSMVYTQQCGTTLLLALILQNLGFRAIPIISYMSQAKKLGSLNAFKSGECNILICSDFVSKGLDIPAVDMVINYNIPWYPNDYIYRVGWNVAHVNVAISFVSPDERRHFNMIEKHIVTIFNESNTTSANKVGIRGHKCEIKANRKKMKVDWTPELHKNFVKAVEQLGIDHTIPSRILDLMKVEGLTRHNVASHLQVIIYLPTVSKNYFNTSFKTVINDKLIFIYFHR