Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34666.t1 | ABN09016.1 | 88.2 | 93 | 11 | 0 | 1 | 93 | 216 | 308 | 1.60E-41 | 178.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34666.t1 | Q9MAA2 | 57.3 | 89 | 38 | 0 | 1 | 89 | 188 | 276 | 4.0e-25 | 115.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34666.t1 | A2Q5Z6 | 88.2 | 93 | 11 | 0 | 1 | 93 | 216 | 308 | 1.2e-41 | 178.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049853 | MS.gene34666 | 0.845965 | 2.92E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34666.t1 | MTR_7g113920 | 88.172 | 93 | 11 | 0 | 1 | 93 | 216 | 308 | 1.69e-56 | 177 |
| MS.gene34666.t1 | MTR_1g082360 | 59.341 | 91 | 35 | 1 | 1 | 89 | 184 | 274 | 1.29e-34 | 120 |
| MS.gene34666.t1 | MTR_5g010090 | 48.750 | 80 | 40 | 1 | 1 | 80 | 262 | 340 | 2.06e-19 | 80.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34666.t1 | AT3G05170 | 57.303 | 89 | 38 | 0 | 1 | 89 | 188 | 276 | 6.65e-31 | 110 |
| MS.gene34666.t1 | AT1G08940 | 48.315 | 89 | 45 | 1 | 2 | 89 | 192 | 280 | 5.38e-25 | 94.7 |
Find 21 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGAACTTCTCAACAATTT+TGG | 0.120449 | 8.1:-46320527 | MS.gene34666:CDS |
| TTCTGATCAGCTACAATATC+AGG | 0.345167 | 8.1:+46320407 | None:intergenic |
| TCCTAATGAGGTGGTTTAGA+TGG | 0.351426 | 8.1:-46320562 | None:intergenic |
| GAGTTCCGTGTGATGCAGTT+TGG | 0.421924 | 8.1:-46320497 | MS.gene34666:CDS |
| TCAAGGGAGCCTTGCTGCCA+TGG | 0.424486 | 8.1:+46320375 | None:intergenic |
| AGTTCCGTGTGATGCAGTTT+GGG | 0.444864 | 8.1:-46320496 | MS.gene34666:CDS |
| CGTGTGATGCAGTTTGGGAC+TGG | 0.458259 | 8.1:-46320491 | MS.gene34666:CDS |
| CAGATGAAGAACTGCTTAAG+TGG | 0.460851 | 8.1:-46320439 | MS.gene34666:CDS |
| GTAGGGACTTTGAACATTCA+AGG | 0.483939 | 8.1:+46320358 | None:intergenic |
| CAAGGGAGCCTTGCTGCCAT+GGG | 0.546859 | 8.1:+46320376 | None:intergenic |
| ATATTGTAGCTGATCAGAAA+TGG | 0.560099 | 8.1:-46320403 | MS.gene34666:CDS |
| AGATGAAGAACTGCTTAAGT+GGG | 0.564810 | 8.1:-46320438 | MS.gene34666:CDS |
| TGATTCTGATGACGAGATTG+TGG | 0.566182 | 8.1:-46320306 | MS.gene34666:CDS |
| TAGGGACTTTGAACATTCAA+GGG | 0.581857 | 8.1:+46320359 | None:intergenic |
| GTGATGCAGTTTGGGACTGG+TGG | 0.583634 | 8.1:-46320488 | MS.gene34666:CDS |
| GACTGGTGGAGATTACAGCT+TGG | 0.612852 | 8.1:-46320474 | MS.gene34666:CDS |
| GATCAGAAATGGTGTGCCCA+TGG | 0.629856 | 8.1:-46320392 | MS.gene34666:CDS |
| CAGTCCCAAACTGCATCACA+CGG | 0.634212 | 8.1:+46320492 | None:intergenic |
| ATGGTGTGCCCATGGCAGCA+AGG | 0.643428 | 8.1:-46320384 | MS.gene34666:CDS |
| GATGAAGAACTGCTTAAGTG+GGG | 0.656412 | 8.1:-46320437 | MS.gene34666:CDS |
| ATGAAGAACTGCTTAAGTGG+GGG | 0.668914 | 8.1:-46320436 | MS.gene34666:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTGAACTTCTCAACAATTT+TGG | - | chr8.1:46320328-46320347 | MS.gene34666:CDS | 25.0% |
| ATATTGTAGCTGATCAGAAA+TGG | - | chr8.1:46320452-46320471 | MS.gene34666:CDS | 30.0% | |
| TGAGATCAAAAAATGCTTCA+AGG | + | chr8.1:46320525-46320544 | None:intergenic | 30.0% | |
| AAAAAATGCTTCAAGGCAGT+AGG | + | chr8.1:46320518-46320537 | None:intergenic | 35.0% | |
| AAAAATGCTTCAAGGCAGTA+GGG | + | chr8.1:46320517-46320536 | None:intergenic | 35.0% | |
| AGATGAAGAACTGCTTAAGT+GGG | - | chr8.1:46320417-46320436 | MS.gene34666:CDS | 35.0% | |
| TAGGGACTTTGAACATTCAA+GGG | + | chr8.1:46320499-46320518 | None:intergenic | 35.0% | |
| TTCTGATCAGCTACAATATC+AGG | + | chr8.1:46320451-46320470 | None:intergenic | 35.0% | |
| ATGAAGAACTGCTTAAGTGG+GGG | - | chr8.1:46320419-46320438 | MS.gene34666:CDS | 40.0% | |
| CAGATGAAGAACTGCTTAAG+TGG | - | chr8.1:46320416-46320435 | MS.gene34666:CDS | 40.0% | |
| GATGAAGAACTGCTTAAGTG+GGG | - | chr8.1:46320418-46320437 | MS.gene34666:CDS | 40.0% | |
| GTAGGGACTTTGAACATTCA+AGG | + | chr8.1:46320500-46320519 | None:intergenic | 40.0% | |
| ! | TGATTCTGATGACGAGATTG+TGG | - | chr8.1:46320549-46320568 | MS.gene34666:CDS | 40.0% |
| AGTTCCGTGTGATGCAGTTT+GGG | - | chr8.1:46320359-46320378 | MS.gene34666:CDS | 45.0% | |
| CAGTCCCAAACTGCATCACA+CGG | + | chr8.1:46320366-46320385 | None:intergenic | 50.0% | |
| GACTGGTGGAGATTACAGCT+TGG | - | chr8.1:46320381-46320400 | MS.gene34666:CDS | 50.0% | |
| GAGTTCCGTGTGATGCAGTT+TGG | - | chr8.1:46320358-46320377 | MS.gene34666:CDS | 50.0% | |
| GATCAGAAATGGTGTGCCCA+TGG | - | chr8.1:46320463-46320482 | MS.gene34666:CDS | 50.0% | |
| ! | CGTGTGATGCAGTTTGGGAC+TGG | - | chr8.1:46320364-46320383 | MS.gene34666:CDS | 55.0% |
| ! | GTGATGCAGTTTGGGACTGG+TGG | - | chr8.1:46320367-46320386 | MS.gene34666:CDS | 55.0% |
| ATGGTGTGCCCATGGCAGCA+AGG | - | chr8.1:46320471-46320490 | MS.gene34666:CDS | 60.0% | |
| CAAGGGAGCCTTGCTGCCAT+GGG | + | chr8.1:46320482-46320501 | None:intergenic | 60.0% | |
| TCAAGGGAGCCTTGCTGCCA+TGG | + | chr8.1:46320483-46320502 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 46320298 | 46320579 | 46320298 | ID=MS.gene34666 |
| chr8.1 | mRNA | 46320298 | 46320579 | 46320298 | ID=MS.gene34666.t1;Parent=MS.gene34666 |
| chr8.1 | exon | 46320298 | 46320579 | 46320298 | ID=MS.gene34666.t1.exon1;Parent=MS.gene34666.t1 |
| chr8.1 | CDS | 46320298 | 46320579 | 46320298 | ID=cds.MS.gene34666.t1;Parent=MS.gene34666.t1 |
| Gene Sequence |
| Protein sequence |
