Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34878.t1 | XP_013446518.1 | 91.4 | 313 | 26 | 1 | 1 | 313 | 1 | 312 | 2.30E-164 | 588.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34878.t1 | A0A072U3X8 | 91.4 | 313 | 26 | 1 | 1 | 313 | 1 | 312 | 1.6e-164 | 588.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050642 | MS.gene34878 | 0.803875 | 2.83E-49 | -1.69E-46 |
| MS.gene052600 | MS.gene34878 | 0.806648 | 7.43E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34878.t1 | MTR_8g080020 | 94.569 | 313 | 16 | 1 | 1 | 313 | 1 | 312 | 0.0 | 597 |
| MS.gene34878.t1 | MTR_2g088950 | 40.574 | 244 | 125 | 6 | 78 | 307 | 103 | 340 | 5.71e-54 | 179 |
| MS.gene34878.t1 | MTR_2g437670 | 40.574 | 244 | 125 | 6 | 78 | 307 | 103 | 340 | 7.87e-54 | 179 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34878.t1 | AT1G67660 | 66.058 | 274 | 81 | 3 | 51 | 312 | 32 | 305 | 1.19e-134 | 384 |
| MS.gene34878.t1 | AT1G67660 | 66.058 | 274 | 81 | 3 | 51 | 312 | 61 | 334 | 1.55e-134 | 385 |
| MS.gene34878.t1 | AT1G67660 | 66.058 | 274 | 81 | 3 | 51 | 312 | 82 | 355 | 2.62e-134 | 385 |
| MS.gene34878.t1 | AT1G13810 | 36.327 | 245 | 136 | 5 | 80 | 309 | 62 | 301 | 2.71e-47 | 161 |
Find 67 sgRNAs with CRISPR-Local
Find 79 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAAGGGAAGCTTTGTTGTT+AGG | 0.238184 | 4.1:+15559643 | MS.gene34878:CDS |
| CTATTACATGCCTCAAGTTC+AGG | 0.257165 | 4.1:+15559471 | MS.gene34878:CDS |
| TCAGGGTCAAATGGAGATTA+TGG | 0.260537 | 4.1:+15559489 | MS.gene34878:CDS |
| AGTTAAGTGTCCTTATAATA+AGG | 0.269945 | 4.1:+15559408 | MS.gene34878:CDS |
| TTGTTAGAAATCGTGAATAT+TGG | 0.278665 | 4.1:+15559569 | MS.gene34878:CDS |
| TTCAAAGAAATGCTATGAAT+TGG | 0.293866 | 4.1:+15559236 | MS.gene34878:CDS |
| TTAGTACTGCATTAGGGTTT+TGG | 0.302003 | 4.1:+15559143 | MS.gene34878:CDS |
| TGTTCTTGGTACCGGAATAT+TGG | 0.306883 | 4.1:+15559384 | MS.gene34878:CDS |
| TCAGGCTTACCCTTATTATA+AGG | 0.309587 | 4.1:-15559418 | None:intergenic |
| AACAACAAAGCTTCCCTTGC+CGG | 0.335954 | 4.1:-15559640 | None:intergenic |
| ATGGGATTCTCAGAGAGTTT+TGG | 0.342835 | 4.1:+15559602 | MS.gene34878:CDS |
| CAAGGGAAGCTTTGTTGTTA+GGG | 0.349473 | 4.1:+15559644 | MS.gene34878:CDS |
| TCAAAGAAATGCTATGAATT+GGG | 0.356628 | 4.1:+15559237 | MS.gene34878:CDS |
| AGCACATTTAGTACTGCATT+AGG | 0.371913 | 4.1:+15559136 | MS.gene34878:CDS |
| GCGTCGCCTGACGGTGTTCT+TGG | 0.374201 | 4.1:+15559370 | MS.gene34878:CDS |
| TGTTAGAAATCGTGAATATT+GGG | 0.374315 | 4.1:+15559570 | MS.gene34878:CDS |
| TTCCGCAACGTTCGGAAGAA+TGG | 0.380577 | 4.1:+15559083 | MS.gene34878:CDS |
| ACACTTAACTTCCAATATTC+CGG | 0.387205 | 4.1:-15559395 | None:intergenic |
| AATGGGTTGATTTGTATTGT+TGG | 0.389762 | 4.1:+15559518 | MS.gene34878:CDS |
| TTCCATGGCAAACCTGTCTC+AGG | 0.395522 | 4.1:-15559436 | None:intergenic |
| ATTCGAAACAGTCTTATGAT+TGG | 0.396472 | 4.1:+15559341 | MS.gene34878:CDS |
| GCACATTTAGTACTGCATTA+GGG | 0.406562 | 4.1:+15559137 | MS.gene34878:CDS |
| TTGGTGGGAAAACGTGATTC+CGG | 0.412330 | 4.1:+15559621 | MS.gene34878:CDS |
| CCTAGCAGAATTAAGCCATA+TGG | 0.413449 | 4.1:+15558941 | MS.gene34878:CDS |
| GGCCGATGTTCCGCAACGTT+CGG | 0.416231 | 4.1:+15559075 | MS.gene34878:CDS |
| GCCTCAAGTTCAGGGTCAAA+TGG | 0.417390 | 4.1:+15559480 | MS.gene34878:CDS |
| CGAAATATAGTACTTCCATT+TGG | 0.450018 | 4.1:-15559544 | None:intergenic |
| GTTGTTAGGGTCTAAAGATC+AGG | 0.451891 | 4.1:+15559657 | MS.gene34878:CDS |
| TGGTGAGTTAGTAGTACATA+TGG | 0.459125 | 4.1:-15558987 | None:intergenic |
| GTTAAGTGTCCTTATAATAA+GGG | 0.460463 | 4.1:+15559409 | MS.gene34878:CDS |
| GTCAATTTATGTGTAGATGC+AGG | 0.462525 | 4.1:-15559694 | None:intergenic |
| TGGGAGGGAGGTGACTTCGA+TGG | 0.467844 | 4.1:+15559306 | MS.gene34878:CDS |
| AGCATTTGCTTCACCGGCAC+CGG | 0.470494 | 4.1:+15559054 | MS.gene34878:CDS |
| GGGAGGGAGGTGACTTCGAT+GGG | 0.473979 | 4.1:+15559307 | MS.gene34878:CDS |
| TATTACATGCCTCAAGTTCA+GGG | 0.475534 | 4.1:+15559472 | MS.gene34878:CDS |
| ATTTACAGCATTTGCTTCAC+CGG | 0.476975 | 4.1:+15559048 | MS.gene34878:CDS |
| GCATCTACACATAAATTGAC+TGG | 0.483317 | 4.1:+15559697 | MS.gene34878:CDS |
| AATGGATATGAGAATTTCAT+CGG | 0.483975 | 4.1:+15558865 | None:intergenic |
| AAAGAAGTTGTTGATGATGT+TGG | 0.484625 | 4.1:-15559007 | None:intergenic |
| TGCGGAACATCGGCCGGTGC+CGG | 0.486267 | 4.1:-15559067 | None:intergenic |
| GAACGTTGCGGAACATCGGC+CGG | 0.493534 | 4.1:-15559073 | None:intergenic |
| AAACAGTCTTATGATTGGCT+TGG | 0.505920 | 4.1:+15559346 | MS.gene34878:CDS |
| AATAAGGGTAAGCCTGAGAC+AGG | 0.506865 | 4.1:+15559424 | MS.gene34878:CDS |
| TTGTATTGTTGGACGCCAAA+TGG | 0.508428 | 4.1:+15559529 | MS.gene34878:CDS |
| TCCATTTGACCCTGAACTTG+AGG | 0.510243 | 4.1:-15559481 | None:intergenic |
| CTATTATGCAGAGAAATTGC+AGG | 0.517198 | 4.1:+15559763 | MS.gene34878:CDS |
| GAATATTGGGATATAATACA+TGG | 0.523100 | 4.1:+15559583 | MS.gene34878:CDS |
| CCATATGGCTTAATTCTGCT+AGG | 0.530559 | 4.1:-15558941 | None:intergenic |
| TGGCTTGGCGCGTCGCCTGA+CGG | 0.530902 | 4.1:+15559361 | MS.gene34878:CDS |
| AGAATGGTTCGCTCTTCGTA+AGG | 0.534956 | 4.1:+15559099 | MS.gene34878:CDS |
| CCGGTACCAAGAACACCGTC+AGG | 0.549096 | 4.1:-15559376 | None:intergenic |
| CCTGACGGTGTTCTTGGTAC+CGG | 0.552937 | 4.1:+15559376 | MS.gene34878:CDS |
| CAAAGAAATGCTATGAATTG+GGG | 0.562081 | 4.1:+15559238 | MS.gene34878:CDS |
| GGAAAACGTGATTCCGGCAA+GGG | 0.564224 | 4.1:+15559627 | MS.gene34878:CDS |
| ACTTGAGGCATGTAATAGAA+TGG | 0.565196 | 4.1:-15559466 | None:intergenic |
| GAATCTAATCAAGCTAGAGT+TGG | 0.567061 | 4.1:-15558894 | None:intergenic |
| TCTAGCTTGATTAGATTCAG+TGG | 0.567949 | 4.1:+15558899 | MS.gene34878:CDS |
| TTCCGAACGTTGCGGAACAT+CGG | 0.575421 | 4.1:-15559077 | None:intergenic |
| GAAGCAAATGCTGTAAATGA+TGG | 0.578930 | 4.1:-15559043 | None:intergenic |
| AGCCTGAGACAGGTTTGCCA+TGG | 0.592845 | 4.1:+15559434 | MS.gene34878:CDS |
| AACCATTCTTCCGAACGTTG+CGG | 0.616608 | 4.1:-15559085 | None:intergenic |
| GGGAAAACGTGATTCCGGCA+AGG | 0.625422 | 4.1:+15559626 | MS.gene34878:CDS |
| GAATTGGGGAGTGCTTAATG+AGG | 0.644725 | 4.1:+15559252 | MS.gene34878:CDS |
| TTGGGGAGTGCTTAATGAGG+CGG | 0.645877 | 4.1:+15559255 | MS.gene34878:CDS |
| GTGGTCGTCGTGCTGAGTTG+TGG | 0.647045 | 4.1:+15559170 | MS.gene34878:CDS |
| AATATTGGGATATAATACAT+GGG | 0.663115 | 4.1:+15559584 | MS.gene34878:CDS |
| GCAAATGCTGTAAATGATGG+AGG | 0.672307 | 4.1:-15559040 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAAGTATACAAAAATTACT+GGG | + | chr4.1:15559287-15559306 | MS.gene34878:CDS | 15.0% |
| !! | AATATTGGGATATAATACAT+GGG | + | chr4.1:15559584-15559603 | MS.gene34878:CDS | 20.0% |
| !!! | ATAAAATGATCTTTTCCATA+TGG | - | chr4.1:15558959-15558978 | None:intergenic | 20.0% |
| !!! | GAAAAGTATACAAAAATTAC+TGG | + | chr4.1:15559286-15559305 | MS.gene34878:CDS | 20.0% |
| ! | AGTTAAGTGTCCTTATAATA+AGG | + | chr4.1:15559408-15559427 | MS.gene34878:CDS | 25.0% |
| ! | GAATATTGGGATATAATACA+TGG | + | chr4.1:15559583-15559602 | MS.gene34878:CDS | 25.0% |
| ! | GTTAAGTGTCCTTATAATAA+GGG | + | chr4.1:15559409-15559428 | MS.gene34878:CDS | 25.0% |
| ! | TCAAAGAAATGCTATGAATT+GGG | + | chr4.1:15559237-15559256 | MS.gene34878:CDS | 25.0% |
| ! | TGTTAGAAATCGTGAATATT+GGG | + | chr4.1:15559570-15559589 | MS.gene34878:CDS | 25.0% |
| ! | TTCAAAGAAATGCTATGAAT+TGG | + | chr4.1:15559236-15559255 | MS.gene34878:CDS | 25.0% |
| ! | TTGTTAGAAATCGTGAATAT+TGG | + | chr4.1:15559569-15559588 | MS.gene34878:CDS | 25.0% |
| ACACTTAACTTCCAATATTC+CGG | - | chr4.1:15559398-15559417 | None:intergenic | 30.0% | |
| AGTATACAAAAATTACTGGG+AGG | + | chr4.1:15559290-15559309 | MS.gene34878:CDS | 30.0% | |
| CAAAGAAATGCTATGAATTG+GGG | + | chr4.1:15559238-15559257 | MS.gene34878:CDS | 30.0% | |
| CGAAATATAGTACTTCCATT+TGG | - | chr4.1:15559547-15559566 | None:intergenic | 30.0% | |
| GTATACAAAAATTACTGGGA+GGG | + | chr4.1:15559291-15559310 | MS.gene34878:CDS | 30.0% | |
| ! | ATTCGAAACAGTCTTATGAT+TGG | + | chr4.1:15559341-15559360 | MS.gene34878:CDS | 30.0% |
| ! | GGAGATTATGGATTTTGAAT+GGG | + | chr4.1:15559501-15559520 | MS.gene34878:CDS | 30.0% |
| ! | TGGAGATTATGGATTTTGAA+TGG | + | chr4.1:15559500-15559519 | MS.gene34878:CDS | 30.0% |
| !! | AAAGAAGTTGTTGATGATGT+TGG | - | chr4.1:15559010-15559029 | None:intergenic | 30.0% |
| !! | AATGGGTTGATTTGTATTGT+TGG | + | chr4.1:15559518-15559537 | MS.gene34878:CDS | 30.0% |
| ACTTGAGGCATGTAATAGAA+TGG | - | chr4.1:15559469-15559488 | None:intergenic | 35.0% | |
| AGCACATTTAGTACTGCATT+AGG | + | chr4.1:15559136-15559155 | MS.gene34878:CDS | 35.0% | |
| ATTTACAGCATTTGCTTCAC+CGG | + | chr4.1:15559048-15559067 | MS.gene34878:CDS | 35.0% | |
| CTATTATGCAGAGAAATTGC+AGG | + | chr4.1:15559763-15559782 | MS.gene34878:CDS | 35.0% | |
| GAAGCAAATGCTGTAAATGA+TGG | - | chr4.1:15559046-15559065 | None:intergenic | 35.0% | |
| GAATCTAATCAAGCTAGAGT+TGG | - | chr4.1:15558897-15558916 | None:intergenic | 35.0% | |
| GCACATTTAGTACTGCATTA+GGG | + | chr4.1:15559137-15559156 | MS.gene34878:CDS | 35.0% | |
| GCATCTACACATAAATTGAC+TGG | + | chr4.1:15559697-15559716 | MS.gene34878:CDS | 35.0% | |
| GTCAATTTATGTGTAGATGC+AGG | - | chr4.1:15559697-15559716 | None:intergenic | 35.0% | |
| TATTACATGCCTCAAGTTCA+GGG | + | chr4.1:15559472-15559491 | MS.gene34878:CDS | 35.0% | |
| TCAGGCTTACCCTTATTATA+AGG | - | chr4.1:15559421-15559440 | None:intergenic | 35.0% | |
| TGGTGAGTTAGTAGTACATA+TGG | - | chr4.1:15558990-15559009 | None:intergenic | 35.0% | |
| ! | AAACAGTCTTATGATTGGCT+TGG | + | chr4.1:15559346-15559365 | MS.gene34878:CDS | 35.0% |
| ! | TAGAATGGCATAGTTTTCCA+TGG | - | chr4.1:15559454-15559473 | None:intergenic | 35.0% |
| ! | TCTAGCTTGATTAGATTCAG+TGG | + | chr4.1:15558899-15558918 | MS.gene34878:CDS | 35.0% |
| ! | TTAGTACTGCATTAGGGTTT+TGG | + | chr4.1:15559143-15559162 | MS.gene34878:CDS | 35.0% |
| CCATATGGCTTAATTCTGCT+AGG | - | chr4.1:15558944-15558963 | None:intergenic | 40.0% | |
| CCTAGCAGAATTAAGCCATA+TGG | + | chr4.1:15558941-15558960 | MS.gene34878:CDS | 40.0% | |
| CTATTACATGCCTCAAGTTC+AGG | + | chr4.1:15559471-15559490 | MS.gene34878:CDS | 40.0% | |
| GCAAATGCTGTAAATGATGG+AGG | - | chr4.1:15559043-15559062 | None:intergenic | 40.0% | |
| GTTGTTAGGGTCTAAAGATC+AGG | + | chr4.1:15559657-15559676 | MS.gene34878:CDS | 40.0% | |
| TACAAAAATTACTGGGAGGG+AGG | + | chr4.1:15559294-15559313 | MS.gene34878:CDS | 40.0% | |
| TCAGGGTCAAATGGAGATTA+TGG | + | chr4.1:15559489-15559508 | MS.gene34878:CDS | 40.0% | |
| TTGTATTGTTGGACGCCAAA+TGG | + | chr4.1:15559529-15559548 | MS.gene34878:CDS | 40.0% | |
| ! | ATGGGATTCTCAGAGAGTTT+TGG | + | chr4.1:15559602-15559621 | MS.gene34878:CDS | 40.0% |
| ! | TGTTCTTGGTACCGGAATAT+TGG | + | chr4.1:15559384-15559403 | MS.gene34878:CDS | 40.0% |
| !! | CAAGGGAAGCTTTGTTGTTA+GGG | + | chr4.1:15559644-15559663 | MS.gene34878:CDS | 40.0% |
| !! | GATTCTCAGAGAGTTTTGGT+GGG | + | chr4.1:15559606-15559625 | MS.gene34878:CDS | 40.0% |
| !!! | ACTGCATTAGGGTTTTGGAA+AGG | + | chr4.1:15559148-15559167 | MS.gene34878:CDS | 40.0% |
| AACAACAAAGCTTCCCTTGC+CGG | - | chr4.1:15559643-15559662 | None:intergenic | 45.0% | |
| AACCATTCTTCCGAACGTTG+CGG | - | chr4.1:15559088-15559107 | None:intergenic | 45.0% | |
| AATAAGGGTAAGCCTGAGAC+AGG | + | chr4.1:15559424-15559443 | MS.gene34878:CDS | 45.0% | |
| AGAATGGTTCGCTCTTCGTA+AGG | + | chr4.1:15559099-15559118 | MS.gene34878:CDS | 45.0% | |
| TCCATTTGACCCTGAACTTG+AGG | - | chr4.1:15559484-15559503 | None:intergenic | 45.0% | |
| TTGGTGGGAAAACGTGATTC+CGG | + | chr4.1:15559621-15559640 | MS.gene34878:CDS | 45.0% | |
| !! | GAATTGGGGAGTGCTTAATG+AGG | + | chr4.1:15559252-15559271 | MS.gene34878:CDS | 45.0% |
| !! | GCAAGGGAAGCTTTGTTGTT+AGG | + | chr4.1:15559643-15559662 | MS.gene34878:CDS | 45.0% |
| !! | GGATTCTCAGAGAGTTTTGG+TGG | + | chr4.1:15559605-15559624 | MS.gene34878:CDS | 45.0% |
| !!! | GCATTAGGGTTTTGGAAAGG+TGG | + | chr4.1:15559151-15559170 | MS.gene34878:CDS | 45.0% |
| GCCTCAAGTTCAGGGTCAAA+TGG | + | chr4.1:15559480-15559499 | MS.gene34878:CDS | 50.0% | |
| GGAAAACGTGATTCCGGCAA+GGG | + | chr4.1:15559627-15559646 | MS.gene34878:CDS | 50.0% | |
| TTCCATGGCAAACCTGTCTC+AGG | - | chr4.1:15559439-15559458 | None:intergenic | 50.0% | |
| TTCCGAACGTTGCGGAACAT+CGG | - | chr4.1:15559080-15559099 | None:intergenic | 50.0% | |
| TTCCGCAACGTTCGGAAGAA+TGG | + | chr4.1:15559083-15559102 | MS.gene34878:CDS | 50.0% | |
| !! | TTGGGGAGTGCTTAATGAGG+CGG | + | chr4.1:15559255-15559274 | MS.gene34878:CDS | 50.0% |
| AGCATTTGCTTCACCGGCAC+CGG | + | chr4.1:15559054-15559073 | MS.gene34878:CDS | 55.0% | |
| AGCCTGAGACAGGTTTGCCA+TGG | + | chr4.1:15559434-15559453 | MS.gene34878:CDS | 55.0% | |
| CCTGACGGTGTTCTTGGTAC+CGG | + | chr4.1:15559376-15559395 | MS.gene34878:CDS | 55.0% | |
| GGGAAAACGTGATTCCGGCA+AGG | + | chr4.1:15559626-15559645 | MS.gene34878:CDS | 55.0% | |
| CCGGTACCAAGAACACCGTC+AGG | - | chr4.1:15559379-15559398 | None:intergenic | 60.0% | |
| GAACGTTGCGGAACATCGGC+CGG | - | chr4.1:15559076-15559095 | None:intergenic | 60.0% | |
| GGCCGATGTTCCGCAACGTT+CGG | + | chr4.1:15559075-15559094 | MS.gene34878:CDS | 60.0% | |
| GGGAGGGAGGTGACTTCGAT+GGG | + | chr4.1:15559307-15559326 | MS.gene34878:CDS | 60.0% | |
| GTGGTCGTCGTGCTGAGTTG+TGG | + | chr4.1:15559170-15559189 | MS.gene34878:CDS | 60.0% | |
| TGGGAGGGAGGTGACTTCGA+TGG | + | chr4.1:15559306-15559325 | MS.gene34878:CDS | 60.0% | |
| GCGTCGCCTGACGGTGTTCT+TGG | + | chr4.1:15559370-15559389 | MS.gene34878:CDS | 65.0% | |
| TGCGGAACATCGGCCGGTGC+CGG | - | chr4.1:15559070-15559089 | None:intergenic | 70.0% | |
| ! | TGGCTTGGCGCGTCGCCTGA+CGG | + | chr4.1:15559361-15559380 | MS.gene34878:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 15558866 | 15559807 | 15558866 | ID=MS.gene34878 |
| chr4.1 | mRNA | 15558866 | 15559807 | 15558866 | ID=MS.gene34878.t1;Parent=MS.gene34878 |
| chr4.1 | exon | 15558866 | 15559807 | 15558866 | ID=MS.gene34878.t1.exon1;Parent=MS.gene34878.t1 |
| chr4.1 | CDS | 15558866 | 15559807 | 15558866 | ID=cds.MS.gene34878.t1;Parent=MS.gene34878.t1 |
| Gene Sequence |
| Protein sequence |
