Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34891.t1 | XP_013446502.1 | 96.1 | 129 | 5 | 0 | 1 | 129 | 1 | 129 | 1.20E-63 | 252.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34891.t1 | I3S2Q7 | 96.1 | 129 | 5 | 0 | 1 | 129 | 1 | 129 | 8.8e-64 | 252.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050277 | MS.gene34891 | 0.811201 | 7.88E-51 | -1.69E-46 |
| MS.gene054937 | MS.gene34891 | 0.801221 | 9.97E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34891.t1 | MTR_8g079498 | 97.938 | 97 | 2 | 0 | 16 | 112 | 16 | 112 | 2.79e-66 | 197 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34891.t1 | AT4G22000 | 68.376 | 117 | 36 | 1 | 1 | 117 | 1 | 116 | 4.73e-54 | 166 |
Find 33 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCTTCATCTCCTTCCTTTC+AGG | 0.120263 | 4.1:+15720847 | None:intergenic |
| CGTAAAGAAGTTCTTCTTGC+TGG | 0.300933 | 4.1:-15720889 | MS.gene34891:CDS |
| TTCTTCTTGCTGGAGAAGAT+TGG | 0.378509 | 4.1:-15720879 | MS.gene34891:CDS |
| AGCTGCTAGTTCAGCGGTAA+AGG | 0.432878 | 4.1:-15721052 | MS.gene34891:CDS |
| GAAAAGGACATAAGGTTGAT+AGG | 0.434665 | 4.1:-15720819 | MS.gene34891:CDS |
| GGCAAACACTGCTAGGTTGA+TGG | 0.439655 | 4.1:-15720779 | MS.gene34891:CDS |
| TTAGTATTAGTGCTCGGCAT+AGG | 0.464094 | 4.1:-15720921 | MS.gene34891:CDS |
| ATGAGTTTGCCAAGGGAGTG+TGG | 0.500959 | 4.1:-15720975 | MS.gene34891:CDS |
| TTGTAAGCAAGCAACATATC+CGG | 0.503383 | 4.1:+15720748 | None:intergenic |
| TAGTATTAGTGCTCGGCATA+GGG | 0.503438 | 4.1:-15720920 | MS.gene34891:CDS |
| TGAGTTTGCCAAGGGAGTGT+GGG | 0.513187 | 4.1:-15720974 | MS.gene34891:CDS |
| GAGATGAAGACGAGGCGAAA+AGG | 0.513253 | 4.1:-15720835 | MS.gene34891:CDS |
| TTTGCTAACTTCTCGCGCCA+TGG | 0.520110 | 4.1:+15721006 | None:intergenic |
| AAGATAAATAGGGTTATGGC+AGG | 0.525263 | 4.1:-15721078 | None:intergenic |
| TGCCGAGCACTAATACTAAG+AGG | 0.532897 | 4.1:+15720925 | None:intergenic |
| GGTATTGGGAGCTAGGGGCA+TGG | 0.536258 | 4.1:-15720951 | MS.gene34891:CDS |
| GAGTTTGCCAAGGGAGTGTG+GGG | 0.545739 | 4.1:-15720973 | MS.gene34891:CDS |
| GACGAGGCGAAAAGGACATA+AGG | 0.547643 | 4.1:-15720827 | MS.gene34891:CDS |
| AGAAGAGGGCAAACACTGCT+AGG | 0.550721 | 4.1:-15720786 | MS.gene34891:CDS |
| AAAGTGGGCGAAGAAGATGA+AGG | 0.570139 | 4.1:-15720719 | MS.gene34891:CDS |
| AAGATGAAGGAAGAAGAGAA+AGG | 0.572789 | 4.1:-15720706 | MS.gene34891:CDS |
| CTACAGAGTATCAATATCCA+TGG | 0.573029 | 4.1:-15721023 | MS.gene34891:CDS |
| CGAGAAGTTAGCAAAATACA+AGG | 0.575656 | 4.1:-15720998 | MS.gene34891:CDS |
| TAGGATAGCTGCGGAGAAGA+GGG | 0.580076 | 4.1:-15720800 | MS.gene34891:CDS |
| AGCCTCTTAGTATTAGTGCT+CGG | 0.583490 | 4.1:-15720927 | MS.gene34891:CDS |
| TAGGTTGATGGATAAGATGC+CGG | 0.589131 | 4.1:-15720767 | MS.gene34891:CDS |
| ATAGGATAGCTGCGGAGAAG+AGG | 0.590998 | 4.1:-15720801 | MS.gene34891:CDS |
| ATACAAGGATGAGTTTGCCA+AGG | 0.595001 | 4.1:-15720983 | MS.gene34891:CDS |
| TACAAGGATGAGTTTGCCAA+GGG | 0.634320 | 4.1:-15720982 | MS.gene34891:CDS |
| TAAGGTTGATAGGATAGCTG+CGG | 0.683219 | 4.1:-15720809 | MS.gene34891:CDS |
| AGGAAAAGCTGCTAGTTCAG+CGG | 0.684482 | 4.1:-15721058 | MS.gene34891:CDS |
| AAAGGAAGGAGATGAAGACG+AGG | 0.696241 | 4.1:-15720843 | MS.gene34891:CDS |
| AGTTTGCCAAGGGAGTGTGG+GGG | 0.755828 | 4.1:-15720972 | MS.gene34891:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TGCTTACAAGAAGAAAAAGT+GGG | - | chr4.1:15721025-15721044 | MS.gene34891:CDS | 30.0% | |
| TTGCTTACAAGAAGAAAAAG+TGG | - | chr4.1:15721024-15721043 | MS.gene34891:CDS | 30.0% | |
| AAGATGAAGGAAGAAGAGAA+AGG | - | chr4.1:15721053-15721072 | MS.gene34891:CDS | 35.0% | |
| CGAGAAGTTAGCAAAATACA+AGG | - | chr4.1:15720761-15720780 | MS.gene34891:CDS | 35.0% | |
| TTGTAAGCAAGCAACATATC+CGG | + | chr4.1:15721014-15721033 | None:intergenic | 35.0% | |
| ! | ATTGGACTTTTGATCCTGAA+AGG | - | chr4.1:15720898-15720917 | MS.gene34891:CDS | 35.0% |
| ! | CTACAGAGTATCAATATCCA+TGG | - | chr4.1:15720736-15720755 | MS.gene34891:CDS | 35.0% |
| ! | GAAAAGGACATAAGGTTGAT+AGG | - | chr4.1:15720940-15720959 | MS.gene34891:CDS | 35.0% |
| AGCCTCTTAGTATTAGTGCT+CGG | - | chr4.1:15720832-15720851 | MS.gene34891:CDS | 40.0% | |
| ATACAAGGATGAGTTTGCCA+AGG | - | chr4.1:15720776-15720795 | MS.gene34891:CDS | 40.0% | |
| CGTAAAGAAGTTCTTCTTGC+TGG | - | chr4.1:15720870-15720889 | MS.gene34891:CDS | 40.0% | |
| TACAAGGATGAGTTTGCCAA+GGG | - | chr4.1:15720777-15720796 | MS.gene34891:CDS | 40.0% | |
| TAGTATTAGTGCTCGGCATA+GGG | - | chr4.1:15720839-15720858 | MS.gene34891:CDS | 40.0% | |
| TTAGTATTAGTGCTCGGCAT+AGG | - | chr4.1:15720838-15720857 | MS.gene34891:CDS | 40.0% | |
| TTCTTCTTGCTGGAGAAGAT+TGG | - | chr4.1:15720880-15720899 | MS.gene34891:CDS | 40.0% | |
| ! | GACTTTTGATCCTGAAAGGA+AGG | - | chr4.1:15720902-15720921 | MS.gene34891:CDS | 40.0% |
| !! | TAAGGTTGATAGGATAGCTG+CGG | - | chr4.1:15720950-15720969 | MS.gene34891:CDS | 40.0% |
| !! | TAGGTTGATGGATAAGATGC+CGG | - | chr4.1:15720992-15721011 | MS.gene34891:CDS | 40.0% |
| AAAGGAAGGAGATGAAGACG+AGG | - | chr4.1:15720916-15720935 | MS.gene34891:CDS | 45.0% | |
| AAAGTGGGCGAAGAAGATGA+AGG | - | chr4.1:15721040-15721059 | MS.gene34891:CDS | 45.0% | |
| AGGAAAAGCTGCTAGTTCAG+CGG | - | chr4.1:15720701-15720720 | MS.gene34891:CDS | 45.0% | |
| GTCTTCATCTCCTTCCTTTC+AGG | + | chr4.1:15720915-15720934 | None:intergenic | 45.0% | |
| TGCCGAGCACTAATACTAAG+AGG | + | chr4.1:15720837-15720856 | None:intergenic | 45.0% | |
| AGAAGAGGGCAAACACTGCT+AGG | - | chr4.1:15720973-15720992 | MS.gene34891:CDS | 50.0% | |
| AGCTGCTAGTTCAGCGGTAA+AGG | - | chr4.1:15720707-15720726 | MS.gene34891:CDS | 50.0% | |
| ATAGGATAGCTGCGGAGAAG+AGG | - | chr4.1:15720958-15720977 | MS.gene34891:CDS | 50.0% | |
| ATGAGTTTGCCAAGGGAGTG+TGG | - | chr4.1:15720784-15720803 | MS.gene34891:CDS | 50.0% | |
| GACGAGGCGAAAAGGACATA+AGG | - | chr4.1:15720932-15720951 | MS.gene34891:CDS | 50.0% | |
| GAGATGAAGACGAGGCGAAA+AGG | - | chr4.1:15720924-15720943 | MS.gene34891:CDS | 50.0% | |
| TAGGATAGCTGCGGAGAAGA+GGG | - | chr4.1:15720959-15720978 | MS.gene34891:CDS | 50.0% | |
| TTTGCTAACTTCTCGCGCCA+TGG | + | chr4.1:15720756-15720775 | None:intergenic | 50.0% | |
| ! | GGCAAACACTGCTAGGTTGA+TGG | - | chr4.1:15720980-15720999 | MS.gene34891:CDS | 50.0% |
| ! | TGAGTTTGCCAAGGGAGTGT+GGG | - | chr4.1:15720785-15720804 | MS.gene34891:CDS | 50.0% |
| ! | AGTTTGCCAAGGGAGTGTGG+GGG | - | chr4.1:15720787-15720806 | MS.gene34891:CDS | 55.0% |
| ! | GAGTTTGCCAAGGGAGTGTG+GGG | - | chr4.1:15720786-15720805 | MS.gene34891:CDS | 55.0% |
| !! | CAAGGGAGTGTGGGGGTATT+GGG | - | chr4.1:15720794-15720813 | MS.gene34891:CDS | 55.0% |
| !! | TGTGGGGGTATTGGGAGCTA+GGG | - | chr4.1:15720802-15720821 | MS.gene34891:CDS | 55.0% |
| CCAATACCCCCACACTCCCT+TGG | + | chr4.1:15720796-15720815 | None:intergenic | 60.0% | |
| ! | CCAAGGGAGTGTGGGGGTAT+TGG | - | chr4.1:15720793-15720812 | MS.gene34891:CDS | 60.0% |
| ! | GGTATTGGGAGCTAGGGGCA+TGG | - | chr4.1:15720808-15720827 | MS.gene34891:CDS | 60.0% |
| !! | GTGGGGGTATTGGGAGCTAG+GGG | - | chr4.1:15720803-15720822 | MS.gene34891:CDS | 60.0% |
| !! | GTGTGGGGGTATTGGGAGCT+AGG | - | chr4.1:15720801-15720820 | MS.gene34891:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 15720696 | 15721085 | 15720696 | ID=MS.gene34891 |
| chr4.1 | mRNA | 15720696 | 15721085 | 15720696 | ID=MS.gene34891.t1;Parent=MS.gene34891 |
| chr4.1 | exon | 15720696 | 15721085 | 15720696 | ID=MS.gene34891.t1.exon1;Parent=MS.gene34891.t1 |
| chr4.1 | CDS | 15720696 | 15721085 | 15720696 | ID=cds.MS.gene34891.t1;Parent=MS.gene34891.t1 |
| Gene Sequence |
| Protein sequence |
