Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054937.t1 | XP_013444087.1 | 100 | 215 | 0 | 0 | 1 | 215 | 1 | 215 | 4.00E-112 | 414.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054937.t1 | P49199 | 87.2 | 219 | 24 | 1 | 1 | 215 | 1 | 219 | 1.0e-100 | 367.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054937.t1 | I3T3T7 | 100.0 | 215 | 0 | 0 | 1 | 215 | 1 | 215 | 2.9e-112 | 414.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050277 | MS.gene054937 | 0.800699 | 1.27E-48 | -1.69E-46 |
| MS.gene050290 | MS.gene054937 | 0.921407 | 3.72E-88 | -1.69E-46 |
| MS.gene050916 | MS.gene054937 | 0.825939 | 3.57E-54 | -1.69E-46 |
| MS.gene051677 | MS.gene054937 | 0.815597 | 8.52E-52 | -1.69E-46 |
| MS.gene051746 | MS.gene054937 | 0.812463 | 4.19E-51 | -1.69E-46 |
| MS.gene051748 | MS.gene054937 | 0.802139 | 6.46E-49 | -1.69E-46 |
| MS.gene051850 | MS.gene054937 | 0.831072 | 2.05E-55 | -1.69E-46 |
| MS.gene051903 | MS.gene054937 | 0.849835 | 2.51E-60 | -1.69E-46 |
| MS.gene052947 | MS.gene054937 | 0.825803 | 3.84E-54 | -1.69E-46 |
| MS.gene053952 | MS.gene054937 | 0.811882 | 5.61E-51 | -1.69E-46 |
| MS.gene054937 | MS.gene056075 | 0.833108 | 6.45E-56 | -1.69E-46 |
| MS.gene054937 | MS.gene056601 | 0.80731 | 5.38E-50 | -1.69E-46 |
| MS.gene054937 | MS.gene056631 | 0.800108 | 1.68E-48 | -1.69E-46 |
| MS.gene054937 | MS.gene056917 | 0.874674 | 5.62E-68 | -1.69E-46 |
| MS.gene054937 | MS.gene057052 | 0.829142 | 6.07E-55 | -1.69E-46 |
| MS.gene054937 | MS.gene057092 | 0.818377 | 2.02E-52 | -1.69E-46 |
| MS.gene054937 | MS.gene057094 | 0.830327 | 3.13E-55 | -1.69E-46 |
| MS.gene054937 | MS.gene057206 | 0.816409 | 5.61E-52 | -1.69E-46 |
| MS.gene054937 | MS.gene058354 | 0.806995 | 6.28E-50 | -1.69E-46 |
| MS.gene054937 | MS.gene058364 | 0.801693 | 7.98E-49 | -1.69E-46 |
| MS.gene054937 | MS.gene058560 | 0.816496 | 5.37E-52 | -1.69E-46 |
| MS.gene054937 | MS.gene059726 | 0.80845 | 3.08E-50 | -1.69E-46 |
| MS.gene054937 | MS.gene06001 | 0.838063 | 3.59E-57 | -1.69E-46 |
| MS.gene054937 | MS.gene060856 | 0.869501 | 2.95E-66 | -1.69E-46 |
| MS.gene054937 | MS.gene061145 | 0.83865 | 2.54E-57 | -1.69E-46 |
| MS.gene054937 | MS.gene06172 | 0.805646 | 1.21E-49 | -1.69E-46 |
| MS.gene054937 | MS.gene065599 | 0.802885 | 4.54E-49 | -1.69E-46 |
| MS.gene054937 | MS.gene065715 | 0.879566 | 1.12E-69 | -1.69E-46 |
| MS.gene054937 | MS.gene065998 | 0.824852 | 6.45E-54 | -1.69E-46 |
| MS.gene054937 | MS.gene066034 | 0.858238 | 9.45E-63 | -1.69E-46 |
| MS.gene054937 | MS.gene066035 | 0.814218 | 1.72E-51 | -1.69E-46 |
| MS.gene054937 | MS.gene066455 | 0.87685 | 1.01E-68 | -1.69E-46 |
| MS.gene054937 | MS.gene068255 | 0.873339 | 1.59E-67 | -1.69E-46 |
| MS.gene054937 | MS.gene06835 | 0.82664 | 2.43E-54 | -1.69E-46 |
| MS.gene054937 | MS.gene069310 | 0.899765 | 1.43E-77 | -1.69E-46 |
| MS.gene054937 | MS.gene069313 | 0.821711 | 3.49E-53 | -1.69E-46 |
| MS.gene054937 | MS.gene069962 | 0.902183 | 1.26E-78 | -1.69E-46 |
| MS.gene054937 | MS.gene070569 | 0.812536 | 4.04E-51 | -1.69E-46 |
| MS.gene054937 | MS.gene070599 | 0.845316 | 4.38E-59 | -1.69E-46 |
| MS.gene054937 | MS.gene070600 | 0.800569 | 1.36E-48 | -1.69E-46 |
| MS.gene054937 | MS.gene071364 | 0.851656 | 7.70E-61 | -1.69E-46 |
| MS.gene054937 | MS.gene071366 | 0.85702 | 2.17E-62 | -1.69E-46 |
| MS.gene054937 | MS.gene071958 | 0.862197 | 6.01E-64 | -1.69E-46 |
| MS.gene054937 | MS.gene072057 | 0.805789 | 1.13E-49 | -1.69E-46 |
| MS.gene054937 | MS.gene07222 | 0.864552 | 1.12E-64 | -1.69E-46 |
| MS.gene054937 | MS.gene073078 | 0.869629 | 2.68E-66 | -1.69E-46 |
| MS.gene054937 | MS.gene073332 | 0.805787 | 1.13E-49 | -1.69E-46 |
| MS.gene054937 | MS.gene074191 | 0.802385 | 5.75E-49 | -1.69E-46 |
| MS.gene054937 | MS.gene074262 | 0.871993 | 4.47E-67 | -1.69E-46 |
| MS.gene054937 | MS.gene074593 | 0.803862 | 2.85E-49 | -1.69E-46 |
| MS.gene054937 | MS.gene074594 | 0.87209 | 4.15E-67 | -1.69E-46 |
| MS.gene054937 | MS.gene09807 | 0.822715 | 2.04E-53 | -1.69E-46 |
| MS.gene054937 | MS.gene20740 | 0.861205 | 1.21E-63 | -1.69E-46 |
| MS.gene054937 | MS.gene21219 | 0.80819 | 3.50E-50 | -1.69E-46 |
| MS.gene054937 | MS.gene21636 | 0.879967 | 8.08E-70 | -1.69E-46 |
| MS.gene054937 | MS.gene22465 | 0.85294 | 3.32E-61 | -1.69E-46 |
| MS.gene054937 | MS.gene23058 | 0.858221 | 9.56E-63 | -1.69E-46 |
| MS.gene054937 | MS.gene23059 | 0.847255 | 1.30E-59 | -1.69E-46 |
| MS.gene054937 | MS.gene23123 | 0.84782 | 9.08E-60 | -1.69E-46 |
| MS.gene054937 | MS.gene23126 | 0.834165 | 3.51E-56 | -1.69E-46 |
| MS.gene054937 | MS.gene23437 | 0.859079 | 5.30E-63 | -1.69E-46 |
| MS.gene054937 | MS.gene24706 | 0.920232 | 1.66E-87 | -1.69E-46 |
| MS.gene054937 | MS.gene24867 | 0.839079 | 1.96E-57 | -1.69E-46 |
| MS.gene054937 | MS.gene24930 | 0.850746 | 1.39E-60 | -1.69E-46 |
| MS.gene054937 | MS.gene25491 | 0.830486 | 2.86E-55 | -1.69E-46 |
| MS.gene054937 | MS.gene27668 | 0.808232 | 3.43E-50 | -1.69E-46 |
| MS.gene054937 | MS.gene27879 | 0.873891 | 1.03E-67 | -1.69E-46 |
| MS.gene054937 | MS.gene28323 | 0.806614 | 7.55E-50 | -1.69E-46 |
| MS.gene054937 | MS.gene29581 | 0.805613 | 1.23E-49 | -1.69E-46 |
| MS.gene054937 | MS.gene30904 | 0.809819 | 1.57E-50 | -1.69E-46 |
| MS.gene054937 | MS.gene31177 | 0.835399 | 1.72E-56 | -1.69E-46 |
| MS.gene054937 | MS.gene31405 | 0.880682 | 4.49E-70 | -1.69E-46 |
| MS.gene054937 | MS.gene31991 | 0.815235 | 1.03E-51 | -1.69E-46 |
| MS.gene054937 | MS.gene33384 | 0.827434 | 1.57E-54 | -1.69E-46 |
| MS.gene054937 | MS.gene34149 | 0.876909 | 9.59E-69 | -1.69E-46 |
| MS.gene054937 | MS.gene34283 | 0.866487 | 2.75E-65 | -1.69E-46 |
| MS.gene054937 | MS.gene34284 | 0.867626 | 1.19E-65 | -1.69E-46 |
| MS.gene054937 | MS.gene34285 | 0.854447 | 1.22E-61 | -1.69E-46 |
| MS.gene054937 | MS.gene34891 | 0.801221 | 9.97E-49 | -1.69E-46 |
| MS.gene054937 | MS.gene35214 | 0.807331 | 5.33E-50 | -1.69E-46 |
| MS.gene054937 | MS.gene35364 | 0.831963 | 1.24E-55 | -1.69E-46 |
| MS.gene054937 | MS.gene35379 | 0.860084 | 2.64E-63 | -1.69E-46 |
| MS.gene054937 | MS.gene35699 | 0.852416 | 4.68E-61 | -1.69E-46 |
| MS.gene054937 | MS.gene36392 | 0.817325 | 3.50E-52 | -1.69E-46 |
| MS.gene054937 | MS.gene36468 | 0.807837 | 4.16E-50 | -1.69E-46 |
| MS.gene054937 | MS.gene36490 | 0.829613 | 4.67E-55 | -1.69E-46 |
| MS.gene054937 | MS.gene37037 | 0.802554 | 5.31E-49 | -1.69E-46 |
| MS.gene054937 | MS.gene37588 | 0.804387 | 2.21E-49 | -1.69E-46 |
| MS.gene054937 | MS.gene39764 | 0.825303 | 5.04E-54 | -1.69E-46 |
| MS.gene054937 | MS.gene40831 | 0.824108 | 9.65E-54 | -1.69E-46 |
| MS.gene054937 | MS.gene41171 | 0.815599 | 8.51E-52 | -1.69E-46 |
| MS.gene054937 | MS.gene41901 | 0.854482 | 1.20E-61 | -1.69E-46 |
| MS.gene054937 | MS.gene41902 | 0.851859 | 6.74E-61 | -1.69E-46 |
| MS.gene054937 | MS.gene42247 | 0.848023 | 7.98E-60 | -1.69E-46 |
| MS.gene054937 | MS.gene43070 | 0.839695 | 1.36E-57 | -1.69E-46 |
| MS.gene054937 | MS.gene43071 | 0.823817 | 1.13E-53 | -1.69E-46 |
| MS.gene054937 | MS.gene44141 | 0.832729 | 8.01E-56 | -1.69E-46 |
| MS.gene054937 | MS.gene45470 | 0.835869 | 1.31E-56 | -1.69E-46 |
| MS.gene054937 | MS.gene46488 | 0.834068 | 3.71E-56 | -1.69E-46 |
| MS.gene054937 | MS.gene46489 | 0.837058 | 6.50E-57 | -1.69E-46 |
| MS.gene054937 | MS.gene46491 | 0.858782 | 6.50E-63 | -1.69E-46 |
| MS.gene054937 | MS.gene47819 | 0.823909 | 1.07E-53 | -1.69E-46 |
| MS.gene054937 | MS.gene48048 | 0.835918 | 1.27E-56 | -1.69E-46 |
| MS.gene054937 | MS.gene49511 | -0.816356 | 5.77E-52 | -1.69E-46 |
| MS.gene054937 | MS.gene50088 | 0.805207 | 1.49E-49 | -1.69E-46 |
| MS.gene054937 | MS.gene50252 | 0.869873 | 2.23E-66 | -1.69E-46 |
| MS.gene054937 | MS.gene51562 | 0.844997 | 5.34E-59 | -1.69E-46 |
| MS.gene054937 | MS.gene54282 | 0.800319 | 1.52E-48 | -1.69E-46 |
| MS.gene054937 | MS.gene55732 | 0.840196 | 1.01E-57 | -1.69E-46 |
| MS.gene054937 | MS.gene57391 | 0.904226 | 1.53E-79 | -1.69E-46 |
| MS.gene054937 | MS.gene57462 | 0.864737 | 9.80E-65 | -1.69E-46 |
| MS.gene054937 | MS.gene57578 | 0.838607 | 2.60E-57 | -1.69E-46 |
| MS.gene054937 | MS.gene58148 | 0.8851 | 1.09E-71 | -1.69E-46 |
| MS.gene054937 | MS.gene58215 | 0.90156 | 2.37E-78 | -1.69E-46 |
| MS.gene054937 | MS.gene58227 | 0.800034 | 1.74E-48 | -1.69E-46 |
| MS.gene054937 | MS.gene58448 | 0.837026 | 6.62E-57 | -1.69E-46 |
| MS.gene054937 | MS.gene58693 | 0.852552 | 4.28E-61 | -1.69E-46 |
| MS.gene054937 | MS.gene58776 | 0.896687 | 2.90E-76 | -1.69E-46 |
| MS.gene054937 | MS.gene60259 | 0.848868 | 4.66E-60 | -1.69E-46 |
| MS.gene054937 | MS.gene60717 | 0.853549 | 2.22E-61 | -1.69E-46 |
| MS.gene054937 | MS.gene61677 | 0.831763 | 1.39E-55 | -1.69E-46 |
| MS.gene054937 | MS.gene62652 | 0.844713 | 6.38E-59 | -1.69E-46 |
| MS.gene054937 | MS.gene62834 | 0.837292 | 5.67E-57 | -1.69E-46 |
| MS.gene054937 | MS.gene65076 | 0.889201 | 3.00E-73 | -1.69E-46 |
| MS.gene054937 | MS.gene66089 | 0.848147 | 7.37E-60 | -1.69E-46 |
| MS.gene054937 | MS.gene66475 | 0.858795 | 6.45E-63 | -1.69E-46 |
| MS.gene054937 | MS.gene67234 | 0.813778 | 2.15E-51 | -1.69E-46 |
| MS.gene054937 | MS.gene67235 | 0.803826 | 2.90E-49 | -1.69E-46 |
| MS.gene054937 | MS.gene67681 | 0.848621 | 5.45E-60 | -1.69E-46 |
| MS.gene054937 | MS.gene70105 | 0.82714 | 1.84E-54 | -1.69E-46 |
| MS.gene054937 | MS.gene70215 | 0.902284 | 1.13E-78 | -1.69E-46 |
| MS.gene054937 | MS.gene70292 | 0.881547 | 2.19E-70 | -1.69E-46 |
| MS.gene054937 | MS.gene72285 | 0.857996 | 1.12E-62 | -1.69E-46 |
| MS.gene054937 | MS.gene75171 | 0.871945 | 4.64E-67 | -1.69E-46 |
| MS.gene054937 | MS.gene75625 | 0.835021 | 2.14E-56 | -1.69E-46 |
| MS.gene054937 | MS.gene76576 | 0.841756 | 3.92E-58 | -1.69E-46 |
| MS.gene054937 | MS.gene77160 | 0.851999 | 6.16E-61 | -1.69E-46 |
| MS.gene054937 | MS.gene77163 | 0.846143 | 2.61E-59 | -1.69E-46 |
| MS.gene054937 | MS.gene77282 | 0.905068 | 6.34E-80 | -1.69E-46 |
| MS.gene054937 | MS.gene78853 | 0.806458 | 8.14E-50 | -1.69E-46 |
| MS.gene054937 | MS.gene80101 | 0.804031 | 2.63E-49 | -1.69E-46 |
| MS.gene054937 | MS.gene81099 | 0.804481 | 2.12E-49 | -1.69E-46 |
| MS.gene054937 | MS.gene81493 | 0.859739 | 3.36E-63 | -1.69E-46 |
| MS.gene054937 | MS.gene83217 | 0.808398 | 3.16E-50 | -1.69E-46 |
| MS.gene054937 | MS.gene83961 | 0.871357 | 7.27E-67 | -1.69E-46 |
| MS.gene054937 | MS.gene84316 | 0.876975 | 9.10E-69 | -1.69E-46 |
| MS.gene054937 | MS.gene84455 | 0.819349 | 1.22E-52 | -1.69E-46 |
| MS.gene054937 | MS.gene85108 | 0.859237 | 4.75E-63 | -1.69E-46 |
| MS.gene054937 | MS.gene85110 | 0.8381 | 3.52E-57 | -1.69E-46 |
| MS.gene054937 | MS.gene86265 | 0.801034 | 1.09E-48 | -1.69E-46 |
| MS.gene054937 | MS.gene87190 | 0.857695 | 1.37E-62 | -1.69E-46 |
| MS.gene054937 | MS.gene87309 | 0.893541 | 5.71E-75 | -1.69E-46 |
| MS.gene054937 | MS.gene88427 | 0.81326 | 2.80E-51 | -1.69E-46 |
| MS.gene054937 | MS.gene88892 | 0.870292 | 1.63E-66 | -1.69E-46 |
| MS.gene054937 | MS.gene89721 | 0.829197 | 5.89E-55 | -1.69E-46 |
| MS.gene054937 | MS.gene90723 | 0.805796 | 1.12E-49 | -1.69E-46 |
| MS.gene054937 | MS.gene90833 | 0.820943 | 5.25E-53 | -1.69E-46 |
| MS.gene054937 | MS.gene91826 | 0.861495 | 9.86E-64 | -1.69E-46 |
| MS.gene054937 | MS.gene92572 | 0.829511 | 4.94E-55 | -1.69E-46 |
| MS.gene054937 | MS.gene94561 | 0.844201 | 8.75E-59 | -1.69E-46 |
| MS.gene054937 | MS.gene95828 | 0.871757 | 5.36E-67 | -1.69E-46 |
| MS.gene054937 | MS.gene98113 | 0.842654 | 2.27E-58 | -1.69E-46 |
| MS.gene054937 | MS.gene99599 | 0.820717 | 5.92E-53 | -1.69E-46 |
| MS.gene054937 | MS.gene99709 | 0.819158 | 1.34E-52 | -1.69E-46 |
| MS.gene054937 | MS.gene99744 | 0.813253 | 2.81E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene054937 | MS.gene30990 | PPI |
| MS.gene054937 | MS.gene23040 | PPI |
| MS.gene054937 | MS.gene06433 | PPI |
| MS.gene054937 | MS.gene013335 | PPI |
| MS.gene049306 | MS.gene054937 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054937.t1 | MTR_8g012325 | 100.000 | 215 | 0 | 0 | 1 | 215 | 1 | 215 | 5.57e-159 | 438 |
| MS.gene054937.t1 | MTR_7g053450 | 91.705 | 217 | 16 | 1 | 1 | 215 | 1 | 217 | 1.17e-126 | 356 |
| MS.gene054937.t1 | MTR_8g012330 | 85.116 | 215 | 30 | 1 | 3 | 215 | 74 | 288 | 2.75e-123 | 350 |
| MS.gene054937.t1 | MTR_4g016670 | 86.528 | 193 | 23 | 2 | 26 | 215 | 26 | 218 | 3.85e-117 | 332 |
| MS.gene054937.t1 | MTR_4g016663 | 73.494 | 83 | 21 | 1 | 134 | 215 | 5 | 87 | 7.61e-36 | 121 |
| MS.gene054937.t1 | MTR_4g016620 | 72.414 | 58 | 15 | 1 | 135 | 191 | 21 | 78 | 6.33e-21 | 84.0 |
| MS.gene054937.t1 | MTR_4g016610 | 58.242 | 91 | 12 | 2 | 125 | 215 | 1045 | 1109 | 4.45e-20 | 88.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054937.t1 | AT5G59240 | 85.714 | 203 | 22 | 1 | 1 | 203 | 1 | 196 | 1.62e-126 | 356 |
| MS.gene054937.t1 | AT5G20290 | 80.476 | 210 | 34 | 1 | 1 | 203 | 1 | 210 | 1.00e-108 | 311 |
Find 50 sgRNAs with CRISPR-Local
Find 117 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATCAAGCTGGCGCTCTTTC+TGG | 0.081596 | 8.3:+77210936 | None:intergenic |
| CACCCTTGCCCTTCTTCTTC+TGG | 0.129125 | 8.3:+77210701 | None:intergenic |
| CAACACTATGGTGTTGATAT+TGG | 0.287835 | 8.3:-77211539 | MS.gene054937:CDS |
| TGTGAAGAGTGCTATTGTTC+AGG | 0.325462 | 8.3:-77211594 | MS.gene054937:CDS |
| GCTTCCCCATGAGAAGTTAC+CGG | 0.437652 | 8.3:+77211697 | None:intergenic |
| ATACGCAGCTACATTCTTGA+AGG | 0.447637 | 8.3:-77210754 | MS.gene054937:intron |
| GTGAGGAGGATTCGTGTTAG+AGG | 0.449573 | 8.3:-77211755 | MS.gene054937:CDS |
| TGTAAACCACATCGAGCAAA+CGG | 0.457912 | 8.3:+77211654 | None:intergenic |
| CAATGGTACCTTCAACACTA+TGG | 0.459815 | 8.3:-77211551 | MS.gene054937:CDS |
| GGTCAATGCGGCAGAGCTGA+TGG | 0.461383 | 8.3:-77210854 | MS.gene054937:CDS |
| ATGCACAAGAGACGTGAAAC+TGG | 0.464049 | 8.3:-77212650 | MS.gene054937:CDS |
| CCTCAATGTGGGCATCAAGC+TGG | 0.484443 | 8.3:+77210924 | None:intergenic |
| TTGAAGGTACCATTGCTTGA+AGG | 0.488302 | 8.3:+77211559 | None:intergenic |
| GCATGCATTTCTTCTCGACC+TGG | 0.488891 | 8.3:-77210875 | MS.gene054937:CDS |
| TATCAACACCATAGTGTTGA+AGG | 0.489500 | 8.3:+77211543 | None:intergenic |
| GAAGTGGAGAGCTTTGAGAT+TGG | 0.494282 | 8.3:-77211723 | MS.gene054937:CDS |
| TCAGCTCTGCCGCATTGACC+AGG | 0.506358 | 8.3:+77210857 | None:intergenic |
| CAGCTACATTCTTGAAGGTA+AGG | 0.509801 | 8.3:-77210749 | MS.gene054937:CDS |
| ATTGAGGAGCAGTTTGGTGG+TGG | 0.510507 | 8.3:-77210908 | MS.gene054937:CDS |
| TGAAGGTACCATTGCTTGAA+GGG | 0.514607 | 8.3:+77211560 | None:intergenic |
| GCCCACATTGAGGAGCAGTT+TGG | 0.515176 | 8.3:-77210914 | MS.gene054937:CDS |
| TGGATACCGGTAACTTCTCA+TGG | 0.515609 | 8.3:-77211703 | MS.gene054937:CDS |
| CAAAACCCGTTTGCTCGATG+TGG | 0.521241 | 8.3:-77211660 | MS.gene054937:CDS |
| CAATGCTTCGAACAATGAGT+TGG | 0.526770 | 8.3:-77211633 | MS.gene054937:CDS |
| CACATTGAGGAGCAGTTTGG+TGG | 0.527285 | 8.3:-77210911 | MS.gene054937:CDS |
| GAAGAAACTCCAGAAGAAGA+AGG | 0.530638 | 8.3:-77210710 | MS.gene054937:CDS |
| GTAAACCACATCGAGCAAAC+GGG | 0.544170 | 8.3:+77211655 | None:intergenic |
| CCAGCTTGATGCCCACATTG+AGG | 0.548092 | 8.3:-77210924 | MS.gene054937:CDS |
| AGGAGGATTCGTGTTAGAGG+TGG | 0.553665 | 8.3:-77211752 | MS.gene054937:CDS |
| TCTGCAATTACAGGAGGCTG+AGG | 0.558767 | 8.3:-77211014 | MS.gene054937:intron |
| ATGCTGCTCCCTTCAAGCAA+TGG | 0.568267 | 8.3:-77211568 | MS.gene054937:CDS |
| CTCCAGAAGAAGAAGGGCAA+GGG | 0.571076 | 8.3:-77210703 | MS.gene054937:CDS |
| GGATACCGGTAACTTCTCAT+GGG | 0.577966 | 8.3:-77211702 | MS.gene054937:CDS |
| GTCAATGCGGCAGAGCTGAT+GGG | 0.580186 | 8.3:-77210853 | MS.gene054937:intron |
| AAGAAACTCCAGAAGAAGAA+GGG | 0.596297 | 8.3:-77210709 | MS.gene054937:CDS |
| ACCAAACTGCTCCTCAATGT+GGG | 0.596753 | 8.3:+77210913 | None:intergenic |
| AAATGTTAGCAAGAAAGAGG+AGG | 0.606920 | 8.3:-77211504 | MS.gene054937:intron |
| CTGGTGGCAAGAAGAAAGCA+TGG | 0.611110 | 8.3:-77212631 | MS.gene054937:CDS |
| GTTTCATCTAGGTACGAGCT+CGG | 0.613407 | 8.3:-77211818 | MS.gene054937:intron |
| ACTCCAGAAGAAGAAGGGCA+AGG | 0.618644 | 8.3:-77210704 | MS.gene054937:CDS |
| CACCAAACTGCTCCTCAATG+TGG | 0.622269 | 8.3:+77210912 | None:intergenic |
| AGAGCTTTGAGATTGGATAC+CGG | 0.636809 | 8.3:-77211716 | MS.gene054937:CDS |
| TTAGAGGTGGAAATGTGAAG+TGG | 0.659708 | 8.3:-77211739 | MS.gene054937:CDS |
| TCTTCTCGACCTGGTCAATG+CGG | 0.660301 | 8.3:-77210866 | MS.gene054937:CDS |
| ATGGAAACAATACAATCAAG+CGG | 0.663363 | 8.3:+77210678 | None:intergenic |
| CACAAGAGACGTGAAACTGG+TGG | 0.674239 | 8.3:-77212647 | MS.gene054937:CDS |
| GATACCGGTAACTTCTCATG+GGG | 0.694944 | 8.3:-77211701 | MS.gene054937:CDS |
| CAAGCAACAAGACAGTGAGG+AGG | 0.706786 | 8.3:-77211769 | MS.gene054937:CDS |
| TATCAAGCAACAAGACAGTG+AGG | 0.731948 | 8.3:-77211772 | MS.gene054937:CDS |
| GTGGCAAGAAGAAAGCATGG+AGG | 0.807428 | 8.3:-77212628 | MS.gene054937:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACTTTTTTTTTTTTTTAATG+TGG | - | chr8.3:77211029-77211048 | MS.gene054937:intron | 10.0% |
| !!! | ATATTTTCTGATAAATAATT+AGG | - | chr8.3:77211464-77211483 | MS.gene054937:intron | 10.0% |
| !!! | ATTTTTTAACTAAGATATTT+TGG | - | chr8.3:77211632-77211651 | MS.gene054937:CDS | 10.0% |
| !!! | CTTTTTTTTTTTTTTAATGT+GGG | - | chr8.3:77211030-77211049 | MS.gene054937:intron | 10.0% |
| !! | CATTATAATATCAAAGAAAA+CGG | + | chr8.3:77212047-77212066 | None:intergenic | 15.0% |
| !! | TATAAAAAAAAGAGTAAAAC+AGG | + | chr8.3:77210897-77210916 | None:intergenic | 15.0% |
| !! | TTGAATATGAAAAAATTCAT+AGG | + | chr8.3:77210746-77210765 | None:intergenic | 15.0% |
| !!! | TTTTGTAATTGTTTTAGATA+TGG | - | chr8.3:77210979-77210998 | MS.gene054937:CDS | 15.0% |
| !! | AAAACATGTATGTTTACTTT+CGG | + | chr8.3:77211327-77211346 | None:intergenic | 20.0% |
| !! | AAAACCAGTATAAAAATTGT+GGG | + | chr8.3:77212125-77212144 | None:intergenic | 20.0% |
| !! | ATAATTAGGTTATAGATCAA+TGG | - | chr8.3:77211478-77211497 | MS.gene054937:intron | 20.0% |
| !! | ATAATTATAGCCAAAAGATA+AGG | + | chr8.3:77211375-77211394 | None:intergenic | 20.0% |
| !! | GTATTATTTAATCCTGAAAT+GGG | - | chr8.3:77211224-77211243 | MS.gene054937:intron | 20.0% |
| !! | TAATTATAGCCAAAAGATAA+GGG | + | chr8.3:77211374-77211393 | None:intergenic | 20.0% |
| !! | TATTATTTAATCCTGAAATG+GGG | - | chr8.3:77211225-77211244 | MS.gene054937:intron | 20.0% |
| !! | TCTCAATGTTATTATTTGTT+GGG | - | chr8.3:77211593-77211612 | MS.gene054937:CDS | 20.0% |
| !! | TTGGTTGTTTAAATGTTATT+TGG | - | chr8.3:77212143-77212162 | MS.gene054937:intron | 20.0% |
| !! | TTTATATATAATGTGGGAAT+TGG | - | chr8.3:77211072-77211091 | MS.gene054937:intron | 20.0% |
| !!! | AGTTTACATTTCTATTTTCT+GGG | - | chr8.3:77211416-77211435 | MS.gene054937:intron | 20.0% |
| !!! | TAGTTTACATTTCTATTTTC+TGG | - | chr8.3:77211415-77211434 | MS.gene054937:intron | 20.0% |
| !!! | TTTATTCATCTGAAATTTTG+TGG | - | chr8.3:77212291-77212310 | MS.gene054937:intron | 20.0% |
| !!! | TTTTTTTTTTTTAATGTGGG+TGG | - | chr8.3:77211033-77211052 | MS.gene054937:intron | 20.0% |
| ! | CAAAACCAGTATAAAAATTG+TGG | + | chr8.3:77212126-77212145 | None:intergenic | 25.0% |
| ! | CGTATTATTTAATCCTGAAA+TGG | - | chr8.3:77211223-77211242 | MS.gene054937:intron | 25.0% |
| ! | GTATTCAAGGAAAAAAAGTT+AGG | + | chr8.3:77212705-77212724 | None:intergenic | 25.0% |
| ! | GTCTCAATGTTATTATTTGT+TGG | - | chr8.3:77211592-77211611 | MS.gene054937:CDS | 25.0% |
| ! | TATTCCTTAGTAATATGTTC+AGG | - | chr8.3:77212418-77212437 | MS.gene054937:intron | 25.0% |
| ! | TCAATTTCAACATATACATG+AGG | + | chr8.3:77212359-77212378 | None:intergenic | 25.0% |
| ! | TTAAAGATCAGTTAATCACT+TGG | - | chr8.3:77212074-77212093 | MS.gene054937:intron | 25.0% |
| ! | TTAATAATCTCTGCAATTAC+AGG | - | chr8.3:77212452-77212471 | MS.gene054937:intron | 25.0% |
| ! | TTGATTGAAGAAAGATTTGA+AGG | + | chr8.3:77210717-77210736 | None:intergenic | 25.0% |
| ! | TTGGGGTTTATATATAATGT+GGG | - | chr8.3:77211066-77211085 | MS.gene054937:intron | 25.0% |
| !! | ATTTCATTTCCCTTATCTTT+TGG | - | chr8.3:77211362-77211381 | MS.gene054937:intron | 25.0% |
| !! | GTTTAAATGTTATTTGGCTT+GGG | - | chr8.3:77212149-77212168 | MS.gene054937:intron | 25.0% |
| !! | TGTTTAAATGTTATTTGGCT+TGG | - | chr8.3:77212148-77212167 | MS.gene054937:intron | 25.0% |
| !! | TTTGGGGTTTATATATAATG+TGG | - | chr8.3:77211065-77211084 | MS.gene054937:intron | 25.0% |
| !!! | ATATTTTGGTTGTTTCATCT+AGG | - | chr8.3:77211646-77211665 | MS.gene054937:CDS | 25.0% |
| !!! | CACAATTTTTATACTGGTTT+TGG | - | chr8.3:77212124-77212143 | MS.gene054937:intron | 25.0% |
| !!! | TGTAATTGTTTTAGATATGG+TGG | - | chr8.3:77210982-77211001 | MS.gene054937:CDS | 25.0% |
| AATTGTGGGCATAAGTATAA+AGG | + | chr8.3:77212111-77212130 | None:intergenic | 30.0% | |
| ATTTCAACATATACATGAGG+TGG | + | chr8.3:77212356-77212375 | None:intergenic | 30.0% | |
| CTTTCCTGAACATATTACTA+AGG | + | chr8.3:77212425-77212444 | None:intergenic | 30.0% | |
| GAAAAATGTTAGCAAGAAAG+AGG | - | chr8.3:77211968-77211987 | MS.gene054937:intron | 30.0% | |
| ! | TATGCCCACAATTTTTATAC+TGG | - | chr8.3:77212118-77212137 | MS.gene054937:intron | 30.0% |
| !!! | GATTGTTGATTTTGAATCTC+AGG | - | chr8.3:77210786-77210805 | MS.gene054937:intron | 30.0% |
| !!! | TAGTTTTAGATGTCTGGATA+AGG | - | chr8.3:77212317-77212336 | MS.gene054937:intron | 30.0% |
| !!! | TGGATTTAGTTTTAGATGTC+TGG | - | chr8.3:77212311-77212330 | MS.gene054937:intron | 30.0% |
| AAATGTTAGCAAGAAAGAGG+AGG | - | chr8.3:77211971-77211990 | MS.gene054937:intron | 35.0% | |
| AAGAAACTCCAGAAGAAGAA+GGG | - | chr8.3:77212766-77212785 | MS.gene054937:intron | 35.0% | |
| ATAATCTCTGCAATTACAGG+AGG | - | chr8.3:77212455-77212474 | MS.gene054937:intron | 35.0% | |
| ATCAGAAAATATGTGCTACC+TGG | + | chr8.3:77211456-77211475 | None:intergenic | 35.0% | |
| GAAAGAGGAGGTAATGTTTA+TGG | - | chr8.3:77211983-77212002 | MS.gene054937:intron | 35.0% | |
| GATTTGTGTGTTGAGATTCT+GGG | - | chr8.3:77210937-77210956 | MS.gene054937:CDS | 35.0% | |
| ! | CTCTAATTTTCTCTGCACAT+GGG | + | chr8.3:77212514-77212533 | None:intergenic | 35.0% |
| ! | TATCAACACCATAGTGTTGA+AGG | + | chr8.3:77211935-77211954 | None:intergenic | 35.0% |
| ! | TGATTTGTGTGTTGAGATTC+TGG | - | chr8.3:77210936-77210955 | MS.gene054937:CDS | 35.0% |
| !! | CAACACTATGGTGTTGATAT+TGG | - | chr8.3:77211936-77211955 | MS.gene054937:intron | 35.0% |
| !! | GTGGGTGGTTATTTGATTTT+GGG | - | chr8.3:77211048-77211067 | MS.gene054937:intron | 35.0% |
| !! | TGGGATAGTTATTTTGTACC+AGG | - | chr8.3:77211435-77211454 | MS.gene054937:intron | 35.0% |
| !! | TGGGTGGTTATTTGATTTTG+GGG | - | chr8.3:77211049-77211068 | MS.gene054937:intron | 35.0% |
| !! | TGTGGGTGGTTATTTGATTT+TGG | - | chr8.3:77211047-77211066 | MS.gene054937:intron | 35.0% |
| !!! | CTTGATAGTTTTGTGTTAGC+TGG | + | chr8.3:77211690-77211709 | None:intergenic | 35.0% |
| AAATCTTCGTACCCCATTTC+AGG | + | chr8.3:77211239-77211258 | None:intergenic | 40.0% | |
| AGAATGTAGCTGCGTATTCA+AGG | + | chr8.3:77212718-77212737 | None:intergenic | 40.0% | |
| ATACGCAGCTACATTCTTGA+AGG | - | chr8.3:77212721-77212740 | MS.gene054937:intron | 40.0% | |
| CAATGCTTCGAACAATGAGT+TGG | - | chr8.3:77211842-77211861 | MS.gene054937:intron | 40.0% | |
| CAATGGTACCTTCAACACTA+TGG | - | chr8.3:77211924-77211943 | MS.gene054937:intron | 40.0% | |
| CAGCTACATTCTTGAAGGTA+AGG | - | chr8.3:77212726-77212745 | MS.gene054937:intron | 40.0% | |
| GAAGAAACTCCAGAAGAAGA+AGG | - | chr8.3:77212765-77212784 | MS.gene054937:intron | 40.0% | |
| TATCAAGCAACAAGACAGTG+AGG | - | chr8.3:77211703-77211722 | MS.gene054937:CDS | 40.0% | |
| TGTAAACCACATCGAGCAAA+CGG | + | chr8.3:77211824-77211843 | None:intergenic | 40.0% | |
| TTAGAGGTGGAAATGTGAAG+TGG | - | chr8.3:77211736-77211755 | MS.gene054937:CDS | 40.0% | |
| ! | GCTCTAATTTTCTCTGCACA+TGG | + | chr8.3:77212515-77212534 | None:intergenic | 40.0% |
| !! | AGAGCTTTGAGATTGGATAC+CGG | - | chr8.3:77211759-77211778 | MS.gene054937:CDS | 40.0% |
| !! | TGAAGGTACCATTGCTTGAA+GGG | + | chr8.3:77211918-77211937 | None:intergenic | 40.0% |
| !! | TGTGAAGAGTGCTATTGTTC+AGG | - | chr8.3:77211881-77211900 | MS.gene054937:intron | 40.0% |
| !! | TTGAAGGTACCATTGCTTGA+AGG | + | chr8.3:77211919-77211938 | None:intergenic | 40.0% |
| !!! | ATAGTTTTGTGTTAGCTGGC+TGG | + | chr8.3:77211686-77211705 | None:intergenic | 40.0% |
| ACCAAACTGCTCCTCAATGT+GGG | + | chr8.3:77212565-77212584 | None:intergenic | 45.0% | |
| ATGCACAAGAGACGTGAAAC+TGG | - | chr8.3:77210825-77210844 | MS.gene054937:intron | 45.0% | |
| CGCACATGAATGTCTGAACT+GGG | - | chr8.3:77211569-77211588 | MS.gene054937:CDS | 45.0% | |
| GACATTCATGTGCGAAGCAT+AGG | + | chr8.3:77211563-77211582 | None:intergenic | 45.0% | |
| GATACCGGTAACTTCTCATG+GGG | - | chr8.3:77211774-77211793 | MS.gene054937:CDS | 45.0% | |
| GCACATGAATGTCTGAACTG+GGG | - | chr8.3:77211570-77211589 | MS.gene054937:CDS | 45.0% | |
| GGATACCGGTAACTTCTCAT+GGG | - | chr8.3:77211773-77211792 | MS.gene054937:CDS | 45.0% | |
| GTAAACCACATCGAGCAAAC+GGG | + | chr8.3:77211823-77211842 | None:intergenic | 45.0% | |
| GTTTCATCTAGGTACGAGCT+CGG | - | chr8.3:77211657-77211676 | MS.gene054937:CDS | 45.0% | |
| TCGCACATGAATGTCTGAAC+TGG | - | chr8.3:77211568-77211587 | MS.gene054937:CDS | 45.0% | |
| TGGATACCGGTAACTTCTCA+TGG | - | chr8.3:77211772-77211791 | MS.gene054937:CDS | 45.0% | |
| !! | GAAGTGGAGAGCTTTGAGAT+TGG | - | chr8.3:77211752-77211771 | MS.gene054937:CDS | 45.0% |
| !!! | TTTTATATATATAATGAAAT+TGG | - | chr8.3:77210910-77210929 | MS.gene054937:CDS | 5.0% |
| !!! | TTTTATTTTATTTTCTTATA+AGG | - | chr8.3:77211288-77211307 | MS.gene054937:intron | 5.0% |
| ACTCCAGAAGAAGAAGGGCA+AGG | - | chr8.3:77212771-77212790 | MS.gene054937:intron | 50.0% | |
| AGGAGGATTCGTGTTAGAGG+TGG | - | chr8.3:77211723-77211742 | MS.gene054937:CDS | 50.0% | |
| ATGCTGCTCCCTTCAAGCAA+TGG | - | chr8.3:77211907-77211926 | MS.gene054937:intron | 50.0% | |
| CAAAACCCGTTTGCTCGATG+TGG | - | chr8.3:77211815-77211834 | MS.gene054937:intron | 50.0% | |
| CAAGCAACAAGACAGTGAGG+AGG | - | chr8.3:77211706-77211725 | MS.gene054937:CDS | 50.0% | |
| CACAAGAGACGTGAAACTGG+TGG | - | chr8.3:77210828-77210847 | MS.gene054937:intron | 50.0% | |
| CACATTGAGGAGCAGTTTGG+TGG | - | chr8.3:77212564-77212583 | MS.gene054937:intron | 50.0% | |
| CACCAAACTGCTCCTCAATG+TGG | + | chr8.3:77212566-77212585 | None:intergenic | 50.0% | |
| CTCCAGAAGAAGAAGGGCAA+GGG | - | chr8.3:77212772-77212791 | MS.gene054937:intron | 50.0% | |
| CTGGTGGCAAGAAGAAAGCA+TGG | - | chr8.3:77210844-77210863 | MS.gene054937:intron | 50.0% | |
| GCATGCATTTCTTCTCGACC+TGG | - | chr8.3:77212600-77212619 | MS.gene054937:intron | 50.0% | |
| GCTTCCCCATGAGAAGTTAC+CGG | + | chr8.3:77211781-77211800 | None:intergenic | 50.0% | |
| GTGAGGAGGATTCGTGTTAG+AGG | - | chr8.3:77211720-77211739 | MS.gene054937:CDS | 50.0% | |
| TCTGCAATTACAGGAGGCTG+AGG | - | chr8.3:77212461-77212480 | MS.gene054937:intron | 50.0% | |
| TCTTCTCGACCTGGTCAATG+CGG | - | chr8.3:77212609-77212628 | MS.gene054937:intron | 50.0% | |
| ! | ATTGAGGAGCAGTTTGGTGG+TGG | - | chr8.3:77212567-77212586 | MS.gene054937:intron | 50.0% |
| ! | GTGGCAAGAAGAAAGCATGG+AGG | - | chr8.3:77210847-77210866 | MS.gene054937:intron | 50.0% |
| CACCCTTGCCCTTCTTCTTC+TGG | + | chr8.3:77212777-77212796 | None:intergenic | 55.0% | |
| CATCAAGCTGGCGCTCTTTC+TGG | + | chr8.3:77212542-77212561 | None:intergenic | 55.0% | |
| CCAGCTTGATGCCCACATTG+AGG | - | chr8.3:77212551-77212570 | MS.gene054937:intron | 55.0% | |
| CCTCAATGTGGGCATCAAGC+TGG | + | chr8.3:77212554-77212573 | None:intergenic | 55.0% | |
| GCCCACATTGAGGAGCAGTT+TGG | - | chr8.3:77212561-77212580 | MS.gene054937:intron | 55.0% | |
| GTCAATGCGGCAGAGCTGAT+GGG | - | chr8.3:77212622-77212641 | MS.gene054937:CDS | 55.0% | |
| GGTCAATGCGGCAGAGCTGA+TGG | - | chr8.3:77212621-77212640 | MS.gene054937:CDS | 60.0% | |
| ! | TCAGCTCTGCCGCATTGACC+AGG | + | chr8.3:77212621-77212640 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 77210693 | 77212804 | 77210693 | ID=MS.gene054937 |
| chr8.3 | mRNA | 77210693 | 77212804 | 77210693 | ID=MS.gene054937.t1;Parent=MS.gene054937 |
| chr8.3 | exon | 77212801 | 77212804 | 77212801 | ID=MS.gene054937.t1.exon1;Parent=MS.gene054937.t1 |
| chr8.3 | CDS | 77212801 | 77212804 | 77212801 | ID=cds.MS.gene054937.t1;Parent=MS.gene054937.t1 |
| chr8.3 | exon | 77212617 | 77212689 | 77212617 | ID=MS.gene054937.t1.exon2;Parent=MS.gene054937.t1 |
| chr8.3 | CDS | 77212617 | 77212689 | 77212617 | ID=cds.MS.gene054937.t1;Parent=MS.gene054937.t1 |
| chr8.3 | exon | 77211505 | 77211829 | 77211505 | ID=MS.gene054937.t1.exon3;Parent=MS.gene054937.t1 |
| chr8.3 | CDS | 77211505 | 77211829 | 77211505 | ID=cds.MS.gene054937.t1;Parent=MS.gene054937.t1 |
| chr8.3 | exon | 77210854 | 77211023 | 77210854 | ID=MS.gene054937.t1.exon4;Parent=MS.gene054937.t1 |
| chr8.3 | CDS | 77210854 | 77211023 | 77210854 | ID=cds.MS.gene054937.t1;Parent=MS.gene054937.t1 |
| chr8.3 | exon | 77210693 | 77210768 | 77210693 | ID=MS.gene054937.t1.exon5;Parent=MS.gene054937.t1 |
| chr8.3 | CDS | 77210693 | 77210768 | 77210693 | ID=cds.MS.gene054937.t1;Parent=MS.gene054937.t1 |
| Gene Sequence |
| Protein sequence |
