Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34893.t1 | XP_013446497.1 | 100 | 69 | 0 | 0 | 1 | 69 | 1 | 69 | 1.80E-29 | 137.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34893.t1 | O22860 | 91.3 | 69 | 6 | 0 | 1 | 69 | 1 | 69 | 2.0e-29 | 129.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34893.t1 | I3SQN4 | 100.0 | 69 | 0 | 0 | 1 | 69 | 1 | 69 | 1.3e-29 | 137.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060702 | MS.gene34893 | 0.805604 | 1.23E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34893.t1 | MTR_8g079485 | 100.000 | 69 | 0 | 0 | 1 | 69 | 1 | 69 | 8.62e-45 | 138 |
| MS.gene34893.t1 | MTR_4g068040 | 94.203 | 69 | 4 | 0 | 1 | 69 | 1 | 69 | 4.72e-42 | 131 |
| MS.gene34893.t1 | MTR_2g018680 | 89.855 | 69 | 7 | 0 | 1 | 69 | 1 | 69 | 7.80e-40 | 125 |
| MS.gene34893.t1 | MTR_4g068040 | 95.238 | 63 | 3 | 0 | 1 | 63 | 1 | 63 | 2.63e-38 | 121 |
| MS.gene34893.t1 | MTR_2g018680 | 90.625 | 64 | 6 | 0 | 1 | 64 | 1 | 64 | 1.30e-36 | 117 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34893.t1 | AT3G59540 | 91.304 | 69 | 6 | 0 | 1 | 69 | 1 | 69 | 1.27e-41 | 130 |
| MS.gene34893.t1 | AT2G43460 | 91.304 | 69 | 6 | 0 | 1 | 69 | 1 | 69 | 1.27e-41 | 130 |
Find 9 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGCAGATCCACGAAATTA+AGG | 0.348108 | 4.1:+15752813 | MS.gene34893:CDS |
| GAAGAAAATCCTTAATTTCG+TGG | 0.409572 | 4.1:-15752822 | None:intergenic |
| TCTTCTGACGGCGCGTAGAA+AGG | 0.443174 | 4.1:+15752840 | MS.gene34893:CDS |
| AGTGAAAATCAAGAGAAGTA+AGG | 0.506775 | 4.1:+15752873 | MS.gene34893:CDS |
| TAAGGATGTGGTGAAGTTCA+AGG | 0.511754 | 4.1:+15752891 | MS.gene34893:CDS |
| TTGAAGCAATCTCTTCCACC+TGG | 0.570275 | 4.1:+15752973 | MS.gene34893:CDS |
| AATCAAGAGAAGTAAGGATG+TGG | 0.579591 | 4.1:+15752879 | MS.gene34893:CDS |
| AGATCTTGAACAGTCAAACC+AGG | 0.648645 | 4.1:-15752991 | None:intergenic |
| TCTTGAACAGTCAAACCAGG+TGG | 0.706952 | 4.1:-15752988 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTAAGGATTTTCTTCTGA+CGG | + | chr4.1:15752828-15752847 | MS.gene34893:CDS | 25.0% |
| !!! | ATGTATTGTGTTGTTTTTTC+AGG | + | chr4.1:15752789-15752808 | MS.gene34893:intron | 25.0% |
| AGTGAAAATCAAGAGAAGTA+AGG | + | chr4.1:15752873-15752892 | MS.gene34893:CDS | 30.0% | |
| GAAGAAAATCCTTAATTTCG+TGG | - | chr4.1:15752825-15752844 | None:intergenic | 30.0% | |
| TCCAATTAACGAAAGAAAGT+GGG | - | chr4.1:15752711-15752730 | None:intergenic | 30.0% | |
| ! | TGAATCTACAACGATGATTT+TGG | + | chr4.1:15752752-15752771 | MS.gene34893:intron | 30.0% |
| AATCAAGAGAAGTAAGGATG+TGG | + | chr4.1:15752879-15752898 | MS.gene34893:CDS | 35.0% | |
| CTCCAATTAACGAAAGAAAG+TGG | - | chr4.1:15752712-15752731 | None:intergenic | 35.0% | |
| TCCCACTTTCTTTCGTTAAT+TGG | + | chr4.1:15752707-15752726 | MS.gene34893:intron | 35.0% | |
| AGATCTTGAACAGTCAAACC+AGG | - | chr4.1:15752994-15753013 | None:intergenic | 40.0% | |
| GAAGCAGATCCACGAAATTA+AGG | + | chr4.1:15752813-15752832 | MS.gene34893:CDS | 40.0% | |
| TAAGGATGTGGTGAAGTTCA+AGG | + | chr4.1:15752891-15752910 | MS.gene34893:CDS | 40.0% | |
| TCTTGAACAGTCAAACCAGG+TGG | - | chr4.1:15752991-15753010 | None:intergenic | 45.0% | |
| TTGAAGCAATCTCTTCCACC+TGG | + | chr4.1:15752973-15752992 | MS.gene34893:CDS | 45.0% | |
| TCTTCTGACGGCGCGTAGAA+AGG | + | chr4.1:15752840-15752859 | MS.gene34893:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 15752699 | 15753017 | 15752699 | ID=MS.gene34893 |
| chr4.1 | mRNA | 15752699 | 15753017 | 15752699 | ID=MS.gene34893.t1;Parent=MS.gene34893 |
| chr4.1 | exon | 15752699 | 15752701 | 15752699 | ID=MS.gene34893.t1.exon1;Parent=MS.gene34893.t1 |
| chr4.1 | CDS | 15752699 | 15752701 | 15752699 | ID=cds.MS.gene34893.t1;Parent=MS.gene34893.t1 |
| chr4.1 | exon | 15752811 | 15753017 | 15752811 | ID=MS.gene34893.t1.exon2;Parent=MS.gene34893.t1 |
| chr4.1 | CDS | 15752811 | 15753017 | 15752811 | ID=cds.MS.gene34893.t1;Parent=MS.gene34893.t1 |
| Gene Sequence |
| Protein sequence |
