AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene060702


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060702.t1 MTR_1g048960 100.000 70 0 0 66 135 27 96 2.25e-47 148
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060702.t1 AT1G72020 67.949 78 24 1 59 135 20 97 9.61e-34 114

Find 43 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAATGGGCATGCTGGTTT+TGG 0.279633 1.3:+31222201 None:intergenic
CTTGGACAAGATGAAGTATC+AGG 0.339393 1.3:-31221741 MS.gene060702:CDS
CCGATGCCACCCCATTGAAA+TGG 0.344424 1.3:+31222186 None:intergenic
ATCCCCATCAAGCAACCAAC+AGG 0.385635 1.3:-31222250 MS.gene060702:CDS
GGCTGGAAAACATTCTTTGT+TGG 0.400005 1.3:-31222165 MS.gene060702:CDS
AACCCTGTTGGTTGCTTGAT+GGG 0.409014 1.3:+31222247 None:intergenic
TTCAATGGGGTGGCATCGGC+TGG 0.413325 1.3:-31222182 MS.gene060702:CDS
ACCAGCATGCCCATTTCAAT+GGG 0.415172 1.3:-31222196 MS.gene060702:CDS
ACCACCAATGGCAATGTTGT+TGG 0.419322 1.3:+31222127 None:intergenic
AACCAGCATGCCCATTTCAA+TGG 0.434819 1.3:-31222197 MS.gene060702:CDS
CAGCAAAAGAAGACAGCATC+TGG 0.440272 1.3:-31221644 MS.gene060702:CDS
TTTGGAGCCTCAAAAGTAGT+TGG 0.442515 1.3:+31222219 None:intergenic
CCCCATTGAAATGGGCATGC+TGG 0.448377 1.3:+31222195 None:intergenic
AAAACATTCTTTGTTGGCTG+TGG 0.450377 1.3:-31222159 MS.gene060702:CDS
GAACCCTGTTGGTTGCTTGA+TGG 0.450900 1.3:+31222246 None:intergenic
CGATGCCACCCCATTGAAAT+GGG 0.461617 1.3:+31222187 None:intergenic
AAGAGGATGGGAGGCGGACA+TGG 0.489860 1.3:-31221806 MS.gene060702:CDS
GTGTTCACAGCTTTCAGCAT+TGG 0.501579 1.3:-31221698 MS.gene060702:CDS
CCATTTCAATGGGGTGGCAT+CGG 0.502593 1.3:-31222186 MS.gene060702:CDS
TGGGTTCAGAATTAAACTCC+TGG 0.515451 1.3:-31222445 MS.gene060702:CDS
TTCAACTGGCGTGAAGAGGA+TGG 0.515922 1.3:-31221819 MS.gene060702:CDS
ACCCTGTTGGTTGCTTGATG+GGG 0.526542 1.3:+31222248 None:intergenic
GCCAACAACATTGCCATTGG+TGG 0.527263 1.3:-31222128 MS.gene060702:intron
TGCATATCACATGTACAACT+TGG 0.529028 1.3:-31221759 MS.gene060702:CDS
TTCATTCAACTGGCGTGAAG+AGG 0.543901 1.3:-31221823 MS.gene060702:CDS
AAACATTCTTTGTTGGCTGT+GGG 0.549894 1.3:-31222158 MS.gene060702:CDS
GGCGTGAAGAGGATGGGAGG+CGG 0.552929 1.3:-31221812 MS.gene060702:CDS
TGGGCCACACCTACCACCAA+TGG 0.561598 1.3:+31222115 None:intergenic
TCAACTGGCGTGAAGAGGAT+GGG 0.574066 1.3:-31221818 MS.gene060702:CDS
GCATATACTCTGGTTCGCTA+TGG 0.578575 1.3:+31221783 None:intergenic
AAAGCCAACAACATTGCCAT+TGG 0.580536 1.3:-31222131 MS.gene060702:CDS
TGGAAAATGACTGCATACAC+AGG 0.585917 1.3:+31221665 None:intergenic
TTCAGAATTAAACTCCTGGA+AGG 0.586793 1.3:-31222441 MS.gene060702:CDS
GCATGCCCATTTCAATGGGG+TGG 0.604000 1.3:-31222192 MS.gene060702:CDS
TTGGACAAGATGAAGTATCA+GGG 0.612137 1.3:-31221740 MS.gene060702:CDS
AGCCTCAAAAGTAGTTGGAG+AGG 0.641705 1.3:+31222224 None:intergenic
TCAGAATTAAACTCCTGGAA+GGG 0.648897 1.3:-31222440 MS.gene060702:CDS
TATGTTGTGACAGTGAGTAG+AGG 0.651791 1.3:-31221848 MS.gene060702:intron
TGATATGCATGCATATACTC+TGG 0.665333 1.3:+31221773 None:intergenic
ACTGGCGTGAAGAGGATGGG+AGG 0.672984 1.3:-31221815 MS.gene060702:CDS
TAGTTGGAGAGGAACCCTGT+TGG 0.689517 1.3:+31222235 None:intergenic
TCCCCATCAAGCAACCAACA+GGG 0.694599 1.3:-31222249 MS.gene060702:CDS
CCAGCATGCCCATTTCAATG+GGG 0.765092 1.3:-31222195 MS.gene060702:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTGAAAGAAAAAAAATCA+TGG + chr1.3:31221733-31221752 None:intergenic 15.0%
!! TTTTTACGTGAAAATGAAAG+AGG + chr1.3:31222132-31222151 None:intergenic 25.0%
AGCCATTGGATTAAAATAGA+TGG + chr1.3:31222101-31222120 None:intergenic 30.0%
TGATTGGGCATACAAAATAT+TGG - chr1.3:31222040-31222059 MS.gene060702:intron 30.0%
! TCTCTTGTTTAAAGATGACT+AGG - chr1.3:31222171-31222190 MS.gene060702:CDS 30.0%
!!! AGCCATCTATTTTAATCCAA+TGG - chr1.3:31222096-31222115 MS.gene060702:intron 30.0%
AAAAAGATGCATCAATGGTG+GGG - chr1.3:31221687-31221706 MS.gene060702:CDS 35.0%
AGAAAAAGATGCATCAATGG+TGG - chr1.3:31221685-31221704 MS.gene060702:CDS 35.0%
ATGTTAAGCCAGTTCTTGAT+TGG - chr1.3:31222024-31222043 MS.gene060702:intron 35.0%
GAAAAAGATGCATCAATGGT+GGG - chr1.3:31221686-31221705 MS.gene060702:CDS 35.0%
GTGAGAAAAAGATGCATCAA+TGG - chr1.3:31221682-31221701 MS.gene060702:CDS 35.0%
TCAGAATTAAACTCCTGGAA+GGG - chr1.3:31221646-31221665 MS.gene060702:CDS 35.0%
TGATATGCATGCATATACTC+TGG + chr1.3:31222316-31222335 None:intergenic 35.0%
TGCATATCACATGTACAACT+TGG - chr1.3:31222327-31222346 MS.gene060702:intron 35.0%
TGTTAAGCCAGTTCTTGATT+GGG - chr1.3:31222025-31222044 MS.gene060702:intron 35.0%
TTCAGAATTAAACTCCTGGA+AGG - chr1.3:31221645-31221664 MS.gene060702:CDS 35.0%
! AAAACATTCTTTGTTGGCTG+TGG - chr1.3:31221927-31221946 MS.gene060702:intron 35.0%
! AAACATTCTTTGTTGGCTGT+GGG - chr1.3:31221928-31221947 MS.gene060702:intron 35.0%
! AGTAGAGGTTTTCATTCAAC+TGG - chr1.3:31222253-31222272 MS.gene060702:CDS 35.0%
! TTGGACAAGATGAAGTATCA+GGG - chr1.3:31222346-31222365 MS.gene060702:intron 35.0%
AAAAGATGCATCAATGGTGG+GGG - chr1.3:31221688-31221707 MS.gene060702:CDS 40.0%
AAAGCCAACAACATTGCCAT+TGG - chr1.3:31221955-31221974 MS.gene060702:intron 40.0%
ATACAAAATATTGGCGCGTG+AGG - chr1.3:31222049-31222068 MS.gene060702:intron 40.0%
GGCTGGAAAACATTCTTTGT+TGG - chr1.3:31221921-31221940 MS.gene060702:intron 40.0%
GTTAGTGGCATACTTGTATG+TGG - chr1.3:31221806-31221825 MS.gene060702:CDS 40.0%
TATGTTGTGACAGTGAGTAG+AGG - chr1.3:31222238-31222257 MS.gene060702:CDS 40.0%
TGGAAAATGACTGCATACAC+AGG + chr1.3:31222424-31222443 None:intergenic 40.0%
TGGGTTCAGAATTAAACTCC+TGG - chr1.3:31221641-31221660 MS.gene060702:CDS 40.0%
TTGTATGCCCAATCAAGAAC+TGG + chr1.3:31222035-31222054 None:intergenic 40.0%
! AAGAGGTTCTAGTTAGCCAT+TGG + chr1.3:31222115-31222134 None:intergenic 40.0%
! CTTGGACAAGATGAAGTATC+AGG - chr1.3:31222345-31222364 MS.gene060702:intron 40.0%
! TTTGGAGCCTCAAAAGTAGT+TGG + chr1.3:31221870-31221889 None:intergenic 40.0%
!! TTCCTCTCCAACTACTTTTG+AGG - chr1.3:31221860-31221879 MS.gene060702:intron 40.0%
AACCAGCATGCCCATTTCAA+TGG - chr1.3:31221889-31221908 MS.gene060702:intron 45.0%
ACCACCAATGGCAATGTTGT+TGG + chr1.3:31221962-31221981 None:intergenic 45.0%
ACCAGCATGCCCATTTCAAT+GGG - chr1.3:31221890-31221909 MS.gene060702:intron 45.0%
AGCCTCAAAAGTAGTTGGAG+AGG + chr1.3:31221865-31221884 None:intergenic 45.0%
AGGGGATGAATCAGTTTCAG+GGG - chr1.3:31221998-31222017 MS.gene060702:intron 45.0%
ATGCTTGGCATCTTCGTTAG+TGG - chr1.3:31221791-31221810 MS.gene060702:CDS 45.0%
CAGCAAAAGAAGACAGCATC+TGG - chr1.3:31222442-31222461 MS.gene060702:CDS 45.0%
GAGGGGATGAATCAGTTTCA+GGG - chr1.3:31221997-31222016 MS.gene060702:intron 45.0%
GCGCTAGAGAGTTATATGCT+TGG - chr1.3:31221776-31221795 MS.gene060702:CDS 45.0%
GGCATACTTGTATGTGGTAG+TGG - chr1.3:31221812-31221831 MS.gene060702:CDS 45.0%
GTGTTCACAGCTTTCAGCAT+TGG - chr1.3:31222388-31222407 MS.gene060702:intron 45.0%
TCTAGCGCACATTCTCATCT+TGG + chr1.3:31221764-31221783 None:intergenic 45.0%
TTCATTCAACTGGCGTGAAG+AGG - chr1.3:31222263-31222282 MS.gene060702:intron 45.0%
! AACCCTGTTGGTTGCTTGAT+GGG + chr1.3:31221842-31221861 None:intergenic 45.0%
! ACAACATTGCCATTGGTGGT+AGG - chr1.3:31221962-31221981 MS.gene060702:intron 45.0%
! CAGATGCTGTCTTCTTTTGC+TGG + chr1.3:31222444-31222463 None:intergenic 45.0%
! GCATATACTCTGGTTCGCTA+TGG + chr1.3:31222306-31222325 None:intergenic 45.0%
!!! TGAAATGGGCATGCTGGTTT+TGG + chr1.3:31221888-31221907 None:intergenic 45.0%
ATCCCCATCAAGCAACCAAC+AGG - chr1.3:31221836-31221855 MS.gene060702:CDS 50.0%
CCAGCATGCCCATTTCAATG+GGG - chr1.3:31221891-31221910 MS.gene060702:intron 50.0%
CCATTTCAATGGGGTGGCAT+CGG - chr1.3:31221900-31221919 MS.gene060702:intron 50.0%
CGATGCCACCCCATTGAAAT+GGG + chr1.3:31221902-31221921 None:intergenic 50.0%
GCCAACAACATTGCCATTGG+TGG - chr1.3:31221958-31221977 MS.gene060702:intron 50.0%
GGAGGGGATGAATCAGTTTC+AGG - chr1.3:31221996-31222015 MS.gene060702:intron 50.0%
TAGTTGGAGAGGAACCCTGT+TGG + chr1.3:31221854-31221873 None:intergenic 50.0%
TCAACTGGCGTGAAGAGGAT+GGG - chr1.3:31222268-31222287 MS.gene060702:intron 50.0%
TCCCCATCAAGCAACCAACA+GGG - chr1.3:31221837-31221856 MS.gene060702:CDS 50.0%
TTCAACTGGCGTGAAGAGGA+TGG - chr1.3:31222267-31222286 MS.gene060702:intron 50.0%
! AACTGATTCATCCCCTCCCA+TGG + chr1.3:31221994-31222013 None:intergenic 50.0%
! ACCCTGTTGGTTGCTTGATG+GGG + chr1.3:31221841-31221860 None:intergenic 50.0%
! ACTGATTCATCCCCTCCCAT+GGG + chr1.3:31221993-31222012 None:intergenic 50.0%
! ATTGCCATTGGTGGTAGGTG+TGG - chr1.3:31221967-31221986 MS.gene060702:intron 50.0%
! GAACCCTGTTGGTTGCTTGA+TGG + chr1.3:31221843-31221862 None:intergenic 50.0%
CCCCATTGAAATGGGCATGC+TGG + chr1.3:31221894-31221913 None:intergenic 55.0%
CCGATGCCACCCCATTGAAA+TGG + chr1.3:31221903-31221922 None:intergenic 55.0%
GCATGCCCATTTCAATGGGG+TGG - chr1.3:31221894-31221913 MS.gene060702:intron 55.0%
!!! TCGCTGACTTTTGCCCTTCC+AGG + chr1.3:31221662-31221681 None:intergenic 55.0%
AAGAGGATGGGAGGCGGACA+TGG - chr1.3:31222280-31222299 MS.gene060702:intron 60.0%
ACTGGCGTGAAGAGGATGGG+AGG - chr1.3:31222271-31222290 MS.gene060702:intron 60.0%
TGGGCCACACCTACCACCAA+TGG + chr1.3:31221974-31221993 None:intergenic 60.0%
TGGTGGTAGGTGTGGCCCAT+GGG - chr1.3:31221975-31221994 MS.gene060702:intron 60.0%
! TTGGTGGTAGGTGTGGCCCA+TGG - chr1.3:31221974-31221993 MS.gene060702:intron 60.0%
!! TTCAATGGGGTGGCATCGGC+TGG - chr1.3:31221904-31221923 MS.gene060702:intron 60.0%
GGCGTGAAGAGGATGGGAGG+CGG - chr1.3:31222274-31222293 MS.gene060702:intron 65.0%
GGTAGGTGTGGCCCATGGGA+GGG - chr1.3:31221979-31221998 MS.gene060702:intron 65.0%
GTAGGTGTGGCCCATGGGAG+GGG - chr1.3:31221980-31221999 MS.gene060702:intron 65.0%
TGGTAGGTGTGGCCCATGGG+AGG - chr1.3:31221978-31221997 MS.gene060702:intron 65.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 31221640 31222468 31221640 ID=MS.gene060702
chr1.3 mRNA 31221640 31222468 31221640 ID=MS.gene060702.t1;Parent=MS.gene060702
chr1.3 exon 31222427 31222468 31222427 ID=MS.gene060702.t1.exon1;Parent=MS.gene060702.t1
chr1.3 CDS 31222427 31222468 31222427 ID=cds.MS.gene060702.t1;Parent=MS.gene060702.t1
chr1.3 exon 31222129 31222276 31222129 ID=MS.gene060702.t1.exon2;Parent=MS.gene060702.t1
chr1.3 CDS 31222129 31222276 31222129 ID=cds.MS.gene060702.t1;Parent=MS.gene060702.t1
chr1.3 exon 31221640 31221857 31221640 ID=MS.gene060702.t1.exon3;Parent=MS.gene060702.t1
chr1.3 CDS 31221640 31221857 31221640 ID=cds.MS.gene060702.t1;Parent=MS.gene060702.t1
Gene Sequence

>MS.gene060702

ATGGGTTCAGAATTAAACTCCTGGAAGGGCAAAAGTCAGCGAGTGAGAAAAAGATGCATCAATGGTGGGGGCTTAGATAAAGCTTAAACTCCATGATTTTTTTTCTTTCAAATAAATTAAACCAAGATGAGAATGTGCGCTAGAGAGTTATATGCTTGGCATCTTCGTTAGTGGCATACTTGTATGTGGTAGTGGAATCCCCATCAAGCAACCAACAGGGTTCCTCTCCAACTACTTTTGAGGCTCCAAAACCAGCATGCCCATTTCAATGGGGTGGCATCGGCTGGAAAACATTCTTTGTTGGCTGTGGGTATGAAAGCCAACAACATTGCCATTGGTGGTAGGTGTGGCCCATGGGAGGGGATGAATCAGTTTCAGGGGTAAATGTTAAGCCAGTTCTTGATTGGGCATACAAAATATTGGCGCGTGAGGTTATGAAAATTACGCATTAATCTAAGCCATCTATTTTAATCCAATGGCTAACTAGAACCTCTTTCATTTTCACGTAAAAAAAGTCATTCTCAATAGATGTCTCTTGTTTAAAGATGACTAGGAAAATTATTGTGTAGTTTTCTACTTTCTAACAAGAAGACATTGTTATGTTGTGACAGTGAGTAGAGGTTTTCATTCAACTGGCGTGAAGAGGATGGGAGGCGGACATGGCCATAGCGAACCAGAGTATATGCATGCATATCACATGTACAACTTGGACAAGATGAAGTATCAGGGTTTGAAAATGTCTCTTGCTGTGTTCACAGCTTTCAGCATTGGTGTTGCAGTTCCTGTGTATGCAGTCATTTTCCAGCAAAAGAAGACAGCATCTGGCTGA

Protein sequence

>MS.gene060702.t1

MGSELNSWKGKSQRWNPHQATNRVPLQLLLRLQNQHAHFNGVASAGKHSLLAVGMKANNIAIGVSRGFHSTGVKRMGGGHGHSEPEYMHAYHMYNLDKMKYQGLKMSLAVFTAFSIGVAVPVYAVIFQQKKTASG