AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35190

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35190.t1 MTR_4g094715 95.966 471 9 1 1 471 1 461 0.0 931
MS.gene35190.t1 MTR_2g032710 74.947 471 110 3 1 471 1 463 0.0 711
MS.gene35190.t1 MTR_1g086390 63.333 390 141 1 74 461 80 469 0.0 542
MS.gene35190.t1 MTR_7g110050 60.621 419 161 3 53 469 46 462 0.0 538
MS.gene35190.t1 MTR_5g034090 43.552 473 226 7 21 458 56 522 9.93e-126 376
MS.gene35190.t1 MTR_8g065010 46.604 427 194 7 58 458 91 509 5.27e-125 374
MS.gene35190.t1 MTR_6g005630 46.348 397 195 6 75 470 45 424 8.93e-122 362
MS.gene35190.t1 MTR_6g005630 43.577 397 183 8 75 470 45 401 1.39e-107 325
MS.gene35190.t1 MTR_5g033680 40.250 400 226 6 60 456 50 439 3.96e-104 318
MS.gene35190.t1 MTR_8g065030 41.117 394 219 6 74 465 64 446 5.95e-102 313
MS.gene35190.t1 MTR_1g052315 44.101 356 185 7 70 422 59 403 5.75e-94 291
MS.gene35190.t1 MTR_7g091850 44.413 358 186 6 74 428 75 422 1.55e-92 288
MS.gene35190.t1 MTR_2g103410 40.106 379 214 7 75 450 59 427 3.66e-92 287
MS.gene35190.t1 MTR_2g060350 43.251 363 191 7 69 427 68 419 8.58e-90 281
MS.gene35190.t1 MTR_7g091840 43.769 329 171 5 69 392 59 378 2.52e-86 270
MS.gene35190.t1 MTR_5g034090 41.926 353 167 5 21 339 56 404 1.68e-81 258
MS.gene35190.t1 MTR_2g103650 36.031 383 233 7 75 453 28 402 1.46e-79 253
MS.gene35190.t1 MTR_3g030540 41.873 363 187 8 76 432 71 415 3.28e-77 248
MS.gene35190.t1 MTR_3g054320 37.245 392 220 10 75 461 30 400 5.44e-76 244
MS.gene35190.t1 MTR_6g028080 36.719 384 222 9 75 453 30 397 3.26e-74 239
MS.gene35190.t1 MTR_3g084380 35.714 406 233 10 53 453 5 387 9.54e-73 235
MS.gene35190.t1 MTR_3g438050 36.883 385 223 8 74 453 26 395 1.50e-72 234
MS.gene35190.t1 MTR_2g049400 37.371 388 224 9 68 453 21 391 1.68e-72 234
MS.gene35190.t1 MTR_1g012390 36.104 385 227 9 75 455 28 397 1.50e-70 229
MS.gene35190.t1 MTR_8g010070 35.385 390 223 10 70 455 26 390 9.75e-70 227
MS.gene35190.t1 MTR_4g084050 35.342 365 209 8 75 428 23 371 6.71e-69 225
MS.gene35190.t1 MTR_5g043350 36.020 397 226 12 66 455 23 398 1.91e-68 224
MS.gene35190.t1 MTR_4g083660 37.275 389 223 13 69 453 3 374 6.02e-68 222
MS.gene35190.t1 MTR_8g010080 35.659 387 220 10 70 453 26 386 5.39e-67 220
MS.gene35190.t1 MTR_1g088160 36.387 382 215 12 68 438 28 392 1.46e-66 219
MS.gene35190.t1 MTR_8g030930 35.754 358 210 7 74 424 26 370 2.23e-66 219
MS.gene35190.t1 MTR_8g010040 35.433 381 218 9 75 452 32 387 7.11e-66 217
MS.gene35190.t1 MTR_0157s0060 35.864 382 217 8 80 455 3 362 7.88e-66 216
MS.gene35190.t1 MTR_4g034020 35.864 382 217 8 80 455 3 362 7.88e-66 216
MS.gene35190.t1 MTR_8g008765 35.671 328 186 5 70 394 26 331 5.52e-65 213
MS.gene35190.t1 MTR_3g084360 35.278 360 211 9 75 428 24 367 7.22e-65 214
MS.gene35190.t1 MTR_2g058640 35.475 358 218 8 74 428 24 371 1.24e-64 214
MS.gene35190.t1 MTR_2g058670 34.943 352 217 7 74 422 24 366 2.99e-64 214
MS.gene35190.t1 MTR_8g010110 33.592 387 229 8 70 453 26 387 7.92e-64 211
MS.gene35190.t1 MTR_2g058840 33.983 359 209 8 74 422 24 364 1.48e-62 209
MS.gene35190.t1 MTR_8g010090 32.817 387 232 9 70 453 26 387 1.57e-62 208
MS.gene35190.t1 MTR_7g113910 35.501 369 151 8 74 440 56 339 5.49e-62 206
MS.gene35190.t1 MTR_2g435640 34.181 354 217 7 74 422 52 394 7.28e-62 207
MS.gene35190.t1 MTR_7g035415 36.508 315 176 7 74 383 39 334 8.80e-62 204
MS.gene35190.t1 MTR_8g009810 35.509 383 226 11 75 453 35 400 1.02e-61 206
MS.gene35190.t1 MTR_2g103410 38.951 267 153 4 75 339 59 317 3.86e-61 203
MS.gene35190.t1 MTR_2g029170 39.506 324 175 10 107 422 23 333 6.15e-60 201
MS.gene35190.t1 MTR_5g097860 36.036 333 182 8 74 394 24 337 7.20e-60 200
MS.gene35190.t1 MTR_1g013730 31.937 382 229 9 75 453 27 380 3.38e-57 194
MS.gene35190.t1 MTR_4g120320 33.249 394 242 9 67 450 38 420 3.87e-57 195
MS.gene35190.t1 MTR_1g013740 31.510 384 232 9 75 455 35 390 1.52e-56 192
MS.gene35190.t1 MTR_8g010050 32.216 388 200 11 70 453 26 354 2.35e-56 191
MS.gene35190.t1 MTR_8g077320 30.711 394 196 10 74 452 32 363 1.61e-51 178
MS.gene35190.t1 MTR_5g063430 32.667 300 181 6 74 369 24 306 6.24e-48 167
MS.gene35190.t1 MTR_8g008700 32.817 323 170 8 133 453 13 290 1.06e-47 166
MS.gene35190.t1 MTR_2g100040 30.447 358 183 11 70 421 20 317 7.54e-41 150
MS.gene35190.t1 MTR_8g031180 37.019 208 121 3 222 423 70 273 8.97e-39 142
MS.gene35190.t1 MTR_4g124980 27.206 408 225 15 56 422 60 436 1.59e-24 106
MS.gene35190.t1 MTR_2g042740 27.415 383 227 14 73 422 54 418 1.19e-23 103
MS.gene35190.t1 MTR_1g100040 26.074 349 211 10 77 391 58 393 9.22e-19 89.0
MS.gene35190.t1 MTR_5g090160 27.532 316 188 9 77 364 46 348 2.97e-18 87.4
MS.gene35190.t1 MTR_1g086030 27.726 321 188 9 77 368 84 389 2.38e-17 84.7
MS.gene35190.t1 MTR_3g449590 26.074 326 200 10 66 364 39 350 2.75e-17 84.3
MS.gene35190.t1 MTR_7g110710 27.245 323 188 10 77 368 45 351 2.89e-17 84.3
MS.gene35190.t1 MTR_7g110710 27.329 322 189 9 77 368 45 351 3.96e-17 84.0
MS.gene35190.t1 MTR_1g100050 25.926 351 209 14 77 391 52 387 5.01e-17 83.6
MS.gene35190.t1 MTR_7g088550 26.282 312 185 10 77 358 52 348 6.58e-17 83.6
MS.gene35190.t1 MTR_8g098840 26.498 317 178 12 77 358 46 342 7.49e-17 83.2
MS.gene35190.t1 MTR_3g096330 26.087 345 206 11 44 358 49 374 8.00e-17 84.0
MS.gene35190.t1 MTR_1g100060 24.928 349 215 10 77 391 95 430 8.49e-17 83.2
MS.gene35190.t1 MTR_1g099850 25.000 352 211 12 77 391 57 392 1.49e-16 82.4
MS.gene35190.t1 MTR_8g028725 24.633 341 215 10 80 391 60 387 3.72e-15 77.8
MS.gene35190.t1 MTR_2g435340 27.492 331 185 13 65 362 31 339 7.13e-15 77.0
MS.gene35190.t1 MTR_4g120730 25.234 321 195 9 77 367 42 347 1.82e-14 75.9
MS.gene35190.t1 MTR_1g086030 28.159 277 157 8 121 368 22 285 1.87e-14 75.5
MS.gene35190.t1 MTR_7g078680 26.045 311 187 10 77 358 57 353 6.82e-14 73.9
MS.gene35190.t1 MTR_2g435340 29.353 201 120 4 173 362 85 274 6.81e-12 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35190.t1 AT1G48100 69.114 463 138 3 1 458 1 463 0.0 659
MS.gene35190.t1 AT1G02460 61.381 391 146 3 74 459 88 478 6.62e-180 513
MS.gene35190.t1 AT1G56710 57.213 409 169 4 54 459 28 433 1.57e-169 484
MS.gene35190.t1 AT4G01890 55.583 403 173 3 63 460 53 454 9.61e-167 478
MS.gene35190.t1 AT5G14650 47.179 390 190 6 75 461 51 427 1.06e-120 360
MS.gene35190.t1 AT1G60590 43.614 415 201 6 75 462 123 531 9.36e-118 356
MS.gene35190.t1 AT1G10640 44.743 409 196 6 74 458 118 520 5.02e-116 351
MS.gene35190.t1 AT1G80170 42.932 382 206 6 71 449 49 421 5.85e-107 325
MS.gene35190.t1 AT3G26610 40.225 445 238 10 21 457 27 451 9.15e-107 325
MS.gene35190.t1 AT1G70500 41.667 396 218 6 74 467 66 450 2.35e-106 324
MS.gene35190.t1 AT1G23460 41.294 402 219 7 62 459 30 418 1.30e-100 308
MS.gene35190.t1 AT1G23460 41.294 402 219 7 62 459 54 442 2.14e-100 308
MS.gene35190.t1 AT3G57510 45.299 351 175 7 77 422 70 408 6.90e-94 291
MS.gene35190.t1 AT3G07970 39.173 411 232 9 35 441 33 429 7.80e-91 283
MS.gene35190.t1 AT2G41850 42.857 350 185 6 77 422 70 408 2.77e-88 276
MS.gene35190.t1 AT1G05660 37.887 388 223 8 70 455 23 394 5.77e-78 248
MS.gene35190.t1 AT1G05650 37.179 390 227 8 68 455 21 394 9.67e-76 243
MS.gene35190.t1 AT3G15720 38.718 390 206 12 75 454 24 390 2.98e-75 243
MS.gene35190.t1 AT3G15720 38.718 390 206 12 75 454 23 389 3.09e-75 243
MS.gene35190.t1 AT2G43890 36.979 384 224 11 67 448 21 388 9.89e-75 240
MS.gene35190.t1 AT4G35670 35.955 356 210 6 75 427 24 364 1.82e-74 239
MS.gene35190.t1 AT5G14650 44.737 266 135 3 75 339 51 305 7.78e-74 235
MS.gene35190.t1 AT2G43870 39.316 351 196 7 75 422 21 357 1.94e-73 236
MS.gene35190.t1 AT3G15720 38.918 388 205 12 75 454 23 386 1.03e-72 237
MS.gene35190.t1 AT2G43880 37.076 383 218 10 75 453 31 394 5.73e-72 233
MS.gene35190.t1 AT1G80170 42.804 271 145 4 71 339 49 311 6.71e-72 231
MS.gene35190.t1 AT5G44840 34.492 374 219 8 74 440 23 377 2.04e-71 231
MS.gene35190.t1 AT1G80140 35.143 350 209 6 107 453 2 336 1.68e-70 227
MS.gene35190.t1 AT2G33160 37.624 404 225 11 56 452 19 402 7.08e-70 234
MS.gene35190.t1 AT5G27530 37.222 360 196 7 74 428 52 386 7.80e-70 229
MS.gene35190.t1 AT3G59850 37.995 379 213 11 75 448 23 384 3.22e-69 226
MS.gene35190.t1 AT1G02790 36.872 358 203 8 68 422 45 382 7.63e-69 226
MS.gene35190.t1 AT3G59850 39.377 353 194 10 75 422 67 404 8.20e-69 226
MS.gene35190.t1 AT1G65570 37.500 384 218 12 75 453 31 397 3.10e-68 223
MS.gene35190.t1 AT2G43860 36.979 384 223 11 75 455 33 400 2.20e-67 221
MS.gene35190.t1 AT1G17150 36.104 385 226 8 74 453 32 401 1.02e-63 212
MS.gene35190.t1 AT1G78400 35.733 389 224 10 74 454 34 404 1.03e-63 212
MS.gene35190.t1 AT4G13760 36.134 357 206 9 74 422 30 372 4.47e-63 210
MS.gene35190.t1 AT1G43080 36.695 357 204 9 74 422 30 372 6.54e-63 209
MS.gene35190.t1 AT5G17200 34.063 411 221 12 71 455 35 421 1.75e-62 209
MS.gene35190.t1 AT1G43090 36.695 357 204 9 74 422 30 372 3.05e-62 209
MS.gene35190.t1 AT5G39910 31.328 399 192 10 74 454 35 369 3.60e-61 204
MS.gene35190.t1 AT3G14040 39.660 353 195 9 75 423 71 409 4.58e-61 206
MS.gene35190.t1 AT4G18180 36.413 368 203 8 68 422 32 381 6.39e-61 205
MS.gene35190.t1 AT2G43880 38.851 296 167 5 75 369 31 313 1.64e-60 201
MS.gene35190.t1 AT5G44830 32.840 338 197 6 106 440 8 318 1.66e-60 201
MS.gene35190.t1 AT3G15720 37.356 348 186 11 116 454 2 326 1.78e-60 203
MS.gene35190.t1 AT1G43100 36.134 357 206 9 74 422 30 372 3.93e-60 203
MS.gene35190.t1 AT2G26620 36.056 355 207 9 74 422 30 370 1.89e-59 201
MS.gene35190.t1 AT3G07850 39.093 353 197 9 75 423 70 408 2.83e-59 201
MS.gene35190.t1 AT2G40310 35.955 356 206 9 75 422 31 372 3.47e-59 200
MS.gene35190.t1 AT3G07820 36.337 344 202 8 83 422 27 357 5.63e-59 199
MS.gene35190.t1 AT3G07830 35.503 338 202 7 88 422 34 358 6.62e-59 199
MS.gene35190.t1 AT3G07840 34.971 346 209 7 80 422 26 358 7.88e-59 199
MS.gene35190.t1 AT2G15460 35.475 358 207 9 74 422 30 372 1.24e-58 198
MS.gene35190.t1 AT4G32380 32.976 373 222 8 89 452 41 394 1.60e-58 199
MS.gene35190.t1 AT5G48140 34.911 338 203 7 88 422 34 357 1.62e-58 198
MS.gene35190.t1 AT2G15450 35.196 358 208 9 74 422 30 372 3.67e-58 197
MS.gene35190.t1 AT2G15470 34.916 358 209 9 74 422 30 372 1.26e-56 193
MS.gene35190.t1 AT4G35670 35.185 270 161 3 158 427 2 257 4.04e-53 180
MS.gene35190.t1 AT4G32370 36.860 293 163 6 89 373 49 327 1.11e-52 181
MS.gene35190.t1 AT4G32375 36.667 270 149 4 158 422 13 265 2.44e-50 177
MS.gene35190.t1 AT4G32380 29.459 370 198 8 89 452 41 353 6.15e-46 165
MS.gene35190.t1 AT4G33440 28.363 342 191 12 48 358 52 370 3.72e-23 102
MS.gene35190.t1 AT3G42950 26.842 380 233 12 73 422 64 428 7.44e-21 95.5
MS.gene35190.t1 AT3G62110 26.437 348 210 11 77 393 45 377 5.29e-20 92.8
MS.gene35190.t1 AT3G62110 26.437 348 210 11 77 393 45 377 5.29e-20 92.8
MS.gene35190.t1 AT1G19170 24.627 402 235 14 63 422 75 450 7.12e-20 92.8
MS.gene35190.t1 AT5G41870 26.000 350 206 12 47 364 21 349 4.16e-19 90.1
MS.gene35190.t1 AT5G49215 25.600 375 235 11 77 422 45 404 1.17e-17 85.5
MS.gene35190.t1 AT5G49215 25.600 375 235 11 77 422 43 402 1.43e-17 85.5
MS.gene35190.t1 AT4G23820 25.868 317 191 8 77 364 43 344 1.99e-17 84.7
MS.gene35190.t1 AT3G48950 23.962 313 192 8 77 358 47 344 5.17e-17 83.6
MS.gene35190.t1 AT2G23900 25.641 312 188 10 77 358 58 355 5.60e-16 80.5
MS.gene35190.t1 AT3G61490 24.839 310 192 10 77 358 62 358 1.17e-14 76.6
MS.gene35190.t1 AT3G61490 24.839 310 192 10 77 358 47 343 1.30e-14 76.3
MS.gene35190.t1 AT3G61490 24.839 310 192 10 77 358 47 343 1.30e-14 76.3
MS.gene35190.t1 AT3G61490 24.839 310 192 10 77 358 47 343 1.30e-14 76.3
MS.gene35190.t1 AT3G61490 24.839 310 192 10 77 358 160 456 1.33e-14 76.6
MS.gene35190.t1 AT4G23500 26.537 309 176 11 80 355 73 363 3.45e-13 72.0

Find 110 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACCTCCAATTAATTGTTTA+AGG 0.064758 4.4:-18901032 MS.gene35190:CDS
CAGTTCCACATGATATTTAA+TGG 0.196238 4.4:-18902030 MS.gene35190:CDS
CCTTCCCTTAAACAATTAAT+TGG 0.200232 4.4:+18901027 None:intergenic
TTCATGGACTATTTATTCTT+TGG 0.220446 4.4:-18903600 MS.gene35190:CDS
TTAATTACCATTGCTTATTA+AGG 0.227433 4.4:+18901900 None:intergenic
CCATAAGCATTCCAACAAAA+AGG 0.243640 4.4:+18901075 None:intergenic
TCTCAAATTAAATATTATAA+AGG 0.297000 4.4:+18903573 None:intergenic
GTTTACCCTGTGAGGTTTGC+AGG 0.305698 4.4:+18902850 None:intergenic
GACGAATCCCGAGGATCATT+TGG 0.309383 4.4:+18903424 None:intergenic
GTTACATCATTTGGAGCAAT+TGG 0.311153 4.4:-18903382 MS.gene35190:CDS
TAGTCCATTCACATACAAAT+TGG 0.324642 4.4:+18902071 None:intergenic
AACAATAGTATTGGTCTATA+AGG 0.326820 4.4:+18901286 None:intergenic
TGTAGAGTCAGGAGTTCTTC+TGG 0.355959 4.4:-18903302 MS.gene35190:CDS
TTGATCTCCATTTCCTTCAA+TGG 0.361661 4.4:+18902886 None:intergenic
GAAAGATCACCCACAGTCTC+AGG 0.377658 4.4:+18901000 None:intergenic
GGATCATTTGAAGACTTAGA+AGG 0.381774 4.4:+18903493 None:intergenic
GGTGATGATTGCATTTCAAT+TGG 0.384128 4.4:-18901762 MS.gene35190:CDS
ACATGGATTCCATCAGTGTT+GGG 0.385395 4.4:+18901955 None:intergenic
CTGTACCATTAAAACTCATT+TGG 0.389055 4.4:+18902912 None:intergenic
ACCTCCAATTAATTGTTTAA+GGG 0.389356 4.4:-18901031 MS.gene35190:CDS
TCTGCCTCTGGCCATGAATC+AGG 0.393547 4.4:+18902971 None:intergenic
TGATCCTCGGGATTCGTCTT+CGG 0.398088 4.4:-18903419 MS.gene35190:CDS
TCAGTGTTGGGGCTAAGTTT+TGG 0.401262 4.4:+18901967 None:intergenic
GGCCATGAATCAGGTCCATC+TGG 0.406021 4.4:+18902980 None:intergenic
TCCCATATGGATCTCATTCA+TGG 0.407523 4.4:-18903616 None:intergenic
GTGGTCCTAGCCACGGGATT+AGG 0.416076 4.4:-18901701 MS.gene35190:intron
TTCCATGCTTTCTTGAATGC+AGG 0.417079 4.4:+18903337 None:intergenic
TCACTACAAGCAAAATGAAT+AGG 0.422913 4.4:+18901171 None:intergenic
TGATTTCTGCTATCTGCCTC+TGG 0.424057 4.4:+18902959 None:intergenic
CTTACTTGAAATACTAATCC+TGG 0.430828 4.4:+18903223 None:intergenic
AATATTTAATTTGAGAAATG+TGG 0.432106 4.4:-18903566 MS.gene35190:CDS
ACAGGAACCATTGAAGGAAA+TGG 0.436139 4.4:-18902893 MS.gene35190:CDS
CCTTTGTCAAACAATAGTAT+TGG 0.440502 4.4:+18901277 None:intergenic
CACCAGATGGACCTGATTCA+TGG 0.440641 4.4:-18902982 MS.gene35190:CDS
AGGAACACTAGGAGAAGAGT+TGG 0.444516 4.4:+18903459 None:intergenic
AACATGGATTCCATCAGTGT+TGG 0.447865 4.4:+18901954 None:intergenic
TCCCGAGGATCATTTGGATA+AGG 0.448420 4.4:+18903430 None:intergenic
AATGGTACAGGAACCATTGA+AGG 0.452512 4.4:-18902899 MS.gene35190:CDS
CCCTTATCCAAATGATCCTC+GGG 0.453352 4.4:-18903431 MS.gene35190:CDS
GCAGCTTGTGCTGTAGAGTC+AGG 0.454931 4.4:-18903313 MS.gene35190:CDS
ATCTTCCCTGCAAACCTCAC+AGG 0.456078 4.4:-18902856 MS.gene35190:CDS
AAAATTTGAGAACATACAAA+TGG 0.456117 4.4:-18901328 MS.gene35190:CDS
GCCATTTGAAGGTGAATTGT+TGG 0.464227 4.4:-18901103 MS.gene35190:CDS
TCCCTTAAACAATTAATTGG+AGG 0.466396 4.4:+18901030 None:intergenic
TTTCATATCTATAGATGATA+CGG 0.467936 4.4:-18902107 MS.gene35190:intron
AAGACATGGCAAGGTGGAAT+GGG 0.468398 4.4:-18901366 MS.gene35190:CDS
TATTTCCACATTTGAAGTCC+CGG 0.469866 4.4:+18901737 None:intergenic
CCCGAGGATCATTTGGATAA+GGG 0.470657 4.4:+18903431 None:intergenic
GTTGAAAACTCACAAGATGT+TGG 0.482732 4.4:-18901934 MS.gene35190:CDS
TAAGTTTGAGACACATGCTT+GGG 0.483234 4.4:+18901437 None:intergenic
TTGAAAACTCACAAGATGTT+GGG 0.483533 4.4:-18901933 MS.gene35190:CDS
TCAGGTGGATGGTATGCTAA+TGG 0.494463 4.4:-18903005 MS.gene35190:intron
AGCTCCAATTTGTATGTGAA+TGG 0.494817 4.4:-18902075 MS.gene35190:CDS
CCAATTAATTGTTTAAGGGA+AGG 0.495839 4.4:-18901027 MS.gene35190:CDS
TTGTACAGCATAGGAAGTCT+TGG 0.499986 4.4:-18901471 MS.gene35190:intron
ATTTGAGAAATGTGGATGGA+AGG 0.500205 4.4:-18903558 MS.gene35190:CDS
AAAGACATGGCAAGGTGGAA+TGG 0.501829 4.4:-18901367 MS.gene35190:CDS
ACCCTTATCCAAATGATCCT+CGG 0.501972 4.4:-18903432 MS.gene35190:CDS
TCCAACAATTCACCTTCAAA+TGG 0.505935 4.4:+18901102 None:intergenic
AATACTAATCCTGGTTTGCA+TGG 0.507411 4.4:+18903232 None:intergenic
ACATATTGATCATTACTCCT+TGG 0.515439 4.4:+18902004 None:intergenic
TAACTTGTGGTCCTAGCCAC+GGG 0.522097 4.4:-18901707 MS.gene35190:CDS
ACTTCAAATGTGGAAATAGC+TGG 0.529833 4.4:-18901732 MS.gene35190:CDS
TCATTTGGAGCAATTGGAGA+TGG 0.537985 4.4:-18903376 MS.gene35190:CDS
GTAACTTGTGGTCCTAGCCA+CGG 0.538941 4.4:-18901708 MS.gene35190:CDS
ATGATATTTAATGGCTGCCA+AGG 0.542871 4.4:-18902021 MS.gene35190:CDS
AACATACAAATGGAGAATGT+AGG 0.544153 4.4:-18901318 MS.gene35190:CDS
ATTTCCACATTTGAAGTCCC+GGG 0.546166 4.4:+18901738 None:intergenic
AAGGAAATGGAGATCAATGG+TGG 0.551216 4.4:-18902880 MS.gene35190:CDS
TTAAGTTTGAGACACATGCT+TGG 0.553610 4.4:+18901436 None:intergenic
AAACTTAGCCCCAACACTGA+TGG 0.554244 4.4:-18901964 MS.gene35190:CDS
GATTGCATTTCAATTGGACC+CGG 0.555852 4.4:-18901756 MS.gene35190:CDS
TTTAATTTGAGAAATGTGGA+TGG 0.556957 4.4:-18903562 MS.gene35190:CDS
TACAACTCCTTAATAAGCAA+TGG 0.557489 4.4:-18901907 MS.gene35190:intron
TTGAAGACTTAGAAGGAACA+AGG 0.561772 4.4:+18903500 None:intergenic
CACCTGCATTCAAGAAAGCA+TGG 0.563512 4.4:-18903339 MS.gene35190:CDS
TTGAAATGTAATCAGGTGGA+TGG 0.567449 4.4:-18903016 MS.gene35190:intron
TTGAAGGAAATGGAGATCAA+TGG 0.574015 4.4:-18902883 MS.gene35190:CDS
TTTACCCTGTGAGGTTTGCA+GGG 0.576342 4.4:+18902851 None:intergenic
TCATTTGGATAAGGGTCAGA+AGG 0.578630 4.4:+18903439 None:intergenic
ATGTACCTAATCCCGTGGCT+AGG 0.592436 4.4:+18901696 None:intergenic
CCAATACTATTGTTTGACAA+AGG 0.596001 4.4:-18901277 MS.gene35190:CDS
GGAAGGTGACCCTGAGACTG+TGG 0.598346 4.4:-18901010 MS.gene35190:CDS
TTAAATATCATGTGGAACTG+AGG 0.598983 4.4:+18902033 None:intergenic
TCTTCCCTGCAAACCTCACA+GGG 0.600082 4.4:-18902855 MS.gene35190:intron
AGGCAGATAGCAGAAATCAA+TGG 0.605028 4.4:-18902955 MS.gene35190:CDS
TGGACCCGGGACTTCAAATG+TGG 0.605054 4.4:-18901742 MS.gene35190:CDS
CTCACAGCTGGACTTCCACA+TGG 0.605942 4.4:+18902591 None:intergenic
TCTTATGACTGTGTCACGGA+CGG 0.610881 4.4:+18901413 None:intergenic
CTAAGAATAAAGACATGGCA+AGG 0.613229 4.4:-18901375 MS.gene35190:CDS
GGTATGCTAATGGCACCAGA+TGG 0.619104 4.4:-18902995 MS.gene35190:CDS
GGCAGCCATTAAATATCATG+TGG 0.622918 4.4:+18902025 None:intergenic
CATGGATTCCATCAGTGTTG+GGG 0.624775 4.4:+18901956 None:intergenic
TAAGGGTCAGAAGGAACACT+AGG 0.625203 4.4:+18903448 None:intergenic
ATTGCATTTCAATTGGACCC+GGG 0.630537 4.4:-18901755 MS.gene35190:CDS
TGAAGACTTAGAAGGAACAA+GGG 0.632788 4.4:+18903501 None:intergenic
ACACTAGGAGAAGAGTTGGA+AGG 0.640153 4.4:+18903463 None:intergenic
AAAGATCACCCACAGTCTCA+GGG 0.643345 4.4:+18901001 None:intergenic
ATGTAATGTACCTAATCCCG+TGG 0.645529 4.4:+18901691 None:intergenic
TGGACCTGATTCATGGCCAG+AGG 0.650317 4.4:-18902975 MS.gene35190:CDS
GAAATAGCTGGTGTAACTTG+TGG 0.654498 4.4:-18901720 MS.gene35190:CDS
GTCATAAGAGAATCAGACAA+TGG 0.654639 4.4:-18901399 MS.gene35190:CDS
ATTCTCTTATGACTGTGTCA+CGG 0.659505 4.4:+18901409 None:intergenic
AAAACTTTGTCAGGACCATG+TGG 0.660723 4.4:-18902606 MS.gene35190:CDS
AGGAAATGGAGATCAATGGT+GGG 0.664389 4.4:-18902879 MS.gene35190:CDS
TACAAACATAACACTCTCTG+AGG 0.682981 4.4:-18901136 MS.gene35190:CDS
AGAATAAAGACATGGCAAGG+TGG 0.690276 4.4:-18901372 MS.gene35190:CDS
CAGTCCGAAGACGAATCCCG+AGG 0.710358 4.4:+18903415 None:intergenic
ATGGACTAAGAATAAAGACA+TGG 0.715833 4.4:-18901380 MS.gene35190:CDS
GAAGGTGACCCTGAGACTGT+GGG 0.722175 4.4:-18901009 MS.gene35190:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAAAATAAATGATATATA+GGG + chr4.4:18901420-18901439 None:intergenic 10.0%
!! ATTCTATATGTAATTAATAT+AGG - chr4.4:18902050-18902069 MS.gene35190:CDS 10.0%
!! TAACAAAATAAATGATATAT+AGG + chr4.4:18901421-18901440 None:intergenic 10.0%
!! TCTATAAATAAATATTTACA+TGG + chr4.4:18901793-18901812 None:intergenic 10.0%
!! TCTCAAATTAAATATTATAA+AGG + chr4.4:18901018-18901037 None:intergenic 10.0%
!! TTCTATATGTAATTAATATA+GGG - chr4.4:18902051-18902070 MS.gene35190:CDS 10.0%
!!! ATATCCTATTTTTATTTTTA+AGG - chr4.4:18902365-18902384 MS.gene35190:intron 10.0%
!!! ATATTTTGAATAGAAAAATT+AGG + chr4.4:18903002-18903021 None:intergenic 10.0%
!!! TAAAATTCGTTTTATTTTTA+GGG - chr4.4:18902396-18902415 MS.gene35190:intron 10.0%
!!! TTAAAATTCGTTTTATTTTT+AGG - chr4.4:18902395-18902414 MS.gene35190:intron 10.0%
!! ATGTCAAAATTATATCAATT+AGG - chr4.4:18902306-18902325 MS.gene35190:intron 15.0%
!! TAGTCCTTAAAAATAAAAAT+AGG + chr4.4:18902372-18902391 None:intergenic 15.0%
!! TCAACTTAATAAAATATGAT+TGG - chr4.4:18901474-18901493 MS.gene35190:intron 15.0%
!! TGTCAAAATTATATCAATTA+GGG - chr4.4:18902307-18902326 MS.gene35190:intron 15.0%
!!! AAAATTCGTTTTATTTTTAG+GGG - chr4.4:18902397-18902416 MS.gene35190:intron 15.0%
!!! AATATTTAATTTGAGAAATG+TGG - chr4.4:18901022-18901041 MS.gene35190:CDS 15.0%
!! AAAATTTGAGAACATACAAA+TGG - chr4.4:18903260-18903279 MS.gene35190:CDS 20.0%
!! AACAAAATTGAAATGTAATC+AGG - chr4.4:18901565-18901584 MS.gene35190:intron 20.0%
!! ATAAACATAAATCAGTCATT+AGG + chr4.4:18902188-18902207 None:intergenic 20.0%
!! GACATTTCTTTAATATAACA+TGG + chr4.4:18902280-18902299 None:intergenic 20.0%
!! TAAACATAAATCAGTCATTA+GGG + chr4.4:18902187-18902206 None:intergenic 20.0%
!! TAATAAGCAATGGTAATTAA+TGG - chr4.4:18902691-18902710 MS.gene35190:intron 20.0%
!! TATAATAACATGATTTGAAC+AGG - chr4.4:18902805-18902824 MS.gene35190:intron 20.0%
!! TTAATTACCATTGCTTATTA+AGG + chr4.4:18902691-18902710 None:intergenic 20.0%
!! TTTCATATCTATAGATGATA+CGG - chr4.4:18902481-18902500 MS.gene35190:intron 20.0%
!!! AAAATGCCAAGTTTTATTTT+TGG + chr4.4:18901947-18901966 None:intergenic 20.0%
!!! AAATGAAACAAAAATTTTCG+AGG - chr4.4:18902335-18902354 MS.gene35190:intron 20.0%
!!! AATGAAACAAAAATTTTCGA+GGG - chr4.4:18902336-18902355 MS.gene35190:intron 20.0%
!!! ACAAAATGGAAAATTTTGAT+AGG + chr4.4:18902157-18902176 None:intergenic 20.0%
!!! ACATCTCGTTTTTATTTTTA+TGG + chr4.4:18902128-18902147 None:intergenic 20.0%
!!! ATAGTGTAATTTGTTTTACA+CGG - chr4.4:18901847-18901866 MS.gene35190:intron 20.0%
!!! ATCACTTCAATTATTTTTTC+AGG - chr4.4:18901332-18901351 MS.gene35190:CDS 20.0%
!!! ATCATAAAAGTGTAATTTTC+TGG + chr4.4:18901314-18901333 None:intergenic 20.0%
! AAAATTGAAATGTAATCAGG+TGG - chr4.4:18901568-18901587 MS.gene35190:intron 25.0%
! ACCTCCAATTAATTGTTTAA+GGG - chr4.4:18903557-18903576 MS.gene35190:CDS 25.0%
! ATTAGGGACTAAAAACAAAA+TGG + chr4.4:18902171-18902190 None:intergenic 25.0%
! CACTAACCAAAAATAAAACT+TGG - chr4.4:18901938-18901957 MS.gene35190:CDS 25.0%
! TACCTCCAATTAATTGTTTA+AGG - chr4.4:18903556-18903575 MS.gene35190:CDS 25.0%
! TGAATAGAAAAATTAGGATC+TGG + chr4.4:18902996-18903015 None:intergenic 25.0%
! TTTAATATAACATGGACTCA+GGG + chr4.4:18902272-18902291 None:intergenic 25.0%
!! AAATAAAACTTGGCATTTTC+AGG - chr4.4:18901948-18901967 MS.gene35190:CDS 25.0%
!! AATAAAACTTGGCATTTTCA+GGG - chr4.4:18901949-18901968 MS.gene35190:CDS 25.0%
!! ATAGAAACAAAGTATCAAAC+AGG + chr4.4:18902739-18902758 None:intergenic 25.0%
!! ATTTTCGATGTTACATCATT+TGG - chr4.4:18901197-18901216 MS.gene35190:CDS 25.0%
!! TAGAAACAAAGTATCAAACA+GGG + chr4.4:18902738-18902757 None:intergenic 25.0%
!! TTCATGGACTATTTATTCTT+TGG - chr4.4:18900988-18901007 MS.gene35190:CDS 25.0%
!! TTGTTAATTTTGTACAGCAT+AGG - chr4.4:18903108-18903127 MS.gene35190:intron 25.0%
!!! AACAATAGTATTGGTCTATA+AGG + chr4.4:18903305-18903324 None:intergenic 25.0%
!!! CAAGTTTTATTTTTGGTTAG+TGG + chr4.4:18901940-18901959 None:intergenic 25.0%
!!! TTTAATTTGAGAAATGTGGA+TGG - chr4.4:18901026-18901045 MS.gene35190:CDS 25.0%
AACATACAAATGGAGAATGT+AGG - chr4.4:18903270-18903289 MS.gene35190:CDS 30.0%
ACATATTGATCATTACTCCT+TGG + chr4.4:18902587-18902606 None:intergenic 30.0%
CAAACAGGGTAAGTAAATAT+TGG + chr4.4:18902724-18902743 None:intergenic 30.0%
CAGTTCCACATGATATTTAA+TGG - chr4.4:18902558-18902577 MS.gene35190:intron 30.0%
CATAAGCATTCCAACAAAAA+GGG + chr4.4:18903515-18903534 None:intergenic 30.0%
CCAATACTATTGTTTGACAA+AGG - chr4.4:18903311-18903330 MS.gene35190:CDS 30.0%
CCAATTAATTGTTTAAGGGA+AGG - chr4.4:18903561-18903580 MS.gene35190:CDS 30.0%
CCTTCCCTTAAACAATTAAT+TGG + chr4.4:18903564-18903583 None:intergenic 30.0%
CCTTTGTCAAACAATAGTAT+TGG + chr4.4:18903314-18903333 None:intergenic 30.0%
CTGTACCATTAAAACTCATT+TGG + chr4.4:18901679-18901698 None:intergenic 30.0%
CTTACTTGAAATACTAATCC+TGG + chr4.4:18901368-18901387 None:intergenic 30.0%
CTTTAATATAACATGGACTC+AGG + chr4.4:18902273-18902292 None:intergenic 30.0%
GTGTCCTACAAAAACATTAA+GGG - chr4.4:18903372-18903391 MS.gene35190:CDS 30.0%
TACAACTCCTTAATAAGCAA+TGG - chr4.4:18902681-18902700 MS.gene35190:intron 30.0%
TAGTCCATTCACATACAAAT+TGG + chr4.4:18902520-18902539 None:intergenic 30.0%
TATTTAATGACTGAGCTGAA+TGG + chr4.4:18902028-18902047 None:intergenic 30.0%
TCACTACAAGCAAAATGAAT+AGG + chr4.4:18903420-18903439 None:intergenic 30.0%
TCCCTTAAACAATTAATTGG+AGG + chr4.4:18903561-18903580 None:intergenic 30.0%
TGTGTCCTACAAAAACATTA+AGG - chr4.4:18903371-18903390 MS.gene35190:CDS 30.0%
TTAAATATCATGTGGAACTG+AGG + chr4.4:18902558-18902577 None:intergenic 30.0%
TTAATATAACATGGACTCAG+GGG + chr4.4:18902271-18902290 None:intergenic 30.0%
TTGAAAACTCACAAGATGTT+GGG - chr4.4:18902655-18902674 MS.gene35190:intron 30.0%
! ATGGACTAAGAATAAAGACA+TGG - chr4.4:18903208-18903227 MS.gene35190:intron 30.0%
!! CAAATGAGTTTTAATGGTAC+AGG - chr4.4:18901677-18901696 MS.gene35190:intron 30.0%
!! CTTGACCAAATGAGTTTTAA+TGG - chr4.4:18901671-18901690 MS.gene35190:intron 30.0%
!! TGTACCCTTAATGTTTTTGT+AGG + chr4.4:18903379-18903398 None:intergenic 30.0%
ACTTCAAATGTGGAAATAGC+TGG - chr4.4:18902856-18902875 MS.gene35190:CDS 35.0%
AGCTCCAATTTGTATGTGAA+TGG - chr4.4:18902513-18902532 MS.gene35190:intron 35.0%
ATGATATTTAATGGCTGCCA+AGG - chr4.4:18902567-18902586 MS.gene35190:intron 35.0%
ATTCTCTTATGACTGTGTCA+CGG + chr4.4:18903182-18903201 None:intergenic 35.0%
ATTGGACGTATGTGTAAAAC+TGG - chr4.4:18901492-18901511 MS.gene35190:intron 35.0%
ATTTGAGAAATGTGGATGGA+AGG - chr4.4:18901030-18901049 MS.gene35190:CDS 35.0%
CCATAAGCATTCCAACAAAA+AGG + chr4.4:18903516-18903535 None:intergenic 35.0%
CTAAGAATAAAGACATGGCA+AGG - chr4.4:18903213-18903232 MS.gene35190:intron 35.0%
GCTGCTTGCTTACATAAAAA+TGG + chr4.4:18902781-18902800 None:intergenic 35.0%
GGATCATTTGAAGACTTAGA+AGG + chr4.4:18901098-18901117 None:intergenic 35.0%
GGTGATGATTGCATTTCAAT+TGG - chr4.4:18902826-18902845 MS.gene35190:intron 35.0%
GTCATAAGAGAATCAGACAA+TGG - chr4.4:18903189-18903208 MS.gene35190:intron 35.0%
GTTGAAAACTCACAAGATGT+TGG - chr4.4:18902654-18902673 MS.gene35190:intron 35.0%
TAATATAACATGGACTCAGG+GGG + chr4.4:18902270-18902289 None:intergenic 35.0%
TACAAACATAACACTCTCTG+AGG - chr4.4:18903452-18903471 MS.gene35190:CDS 35.0%
TCAAAAAAGTTTACCCTGTG+AGG + chr4.4:18901749-18901768 None:intergenic 35.0%
TCCAACAATTCACCTTCAAA+TGG + chr4.4:18903489-18903508 None:intergenic 35.0%
TGAAGACTTAGAAGGAACAA+GGG + chr4.4:18901090-18901109 None:intergenic 35.0%
TTGAAATGTAATCAGGTGGA+TGG - chr4.4:18901572-18901591 MS.gene35190:intron 35.0%
TTGAAGACTTAGAAGGAACA+AGG + chr4.4:18901091-18901110 None:intergenic 35.0%
TTGAAGGAAATGGAGATCAA+TGG - chr4.4:18901705-18901724 MS.gene35190:CDS 35.0%
TTGATCTCCATTTCCTTCAA+TGG + chr4.4:18901705-18901724 None:intergenic 35.0%
! AATACTAATCCTGGTTTGCA+TGG + chr4.4:18901359-18901378 None:intergenic 35.0%
! CATCTTGTGAGTTTTCAACA+TGG + chr4.4:18902653-18902672 None:intergenic 35.0%
! CCTTTTTGTTGGAATGCTTA+TGG - chr4.4:18903513-18903532 MS.gene35190:CDS 35.0%
! GGAAGATACTTTCTTTTGCT+TGG + chr4.4:18901069-18901088 None:intergenic 35.0%
! GTTACATCATTTGGAGCAAT+TGG - chr4.4:18901206-18901225 MS.gene35190:CDS 35.0%
! TAAGTTTGAGACACATGCTT+GGG + chr4.4:18903154-18903173 None:intergenic 35.0%
! TATTTCCACATTTGAAGTCC+CGG + chr4.4:18902854-18902873 None:intergenic 35.0%
! TTAAGTTTGAGACACATGCT+TGG + chr4.4:18903155-18903174 None:intergenic 35.0%
AAAACTTTGTCAGGACCATG+TGG - chr4.4:18901982-18902001 MS.gene35190:CDS 40.0%
AACATGGATTCCATCAGTGT+TGG + chr4.4:18902637-18902656 None:intergenic 40.0%
AAGGAAATGGAGATCAATGG+TGG - chr4.4:18901708-18901727 MS.gene35190:CDS 40.0%
AATGGTACAGGAACCATTGA+AGG - chr4.4:18901689-18901708 MS.gene35190:intron 40.0%
ACAGGAACCATTGAAGGAAA+TGG - chr4.4:18901695-18901714 MS.gene35190:intron 40.0%
ACATGGATTCCATCAGTGTT+GGG + chr4.4:18902636-18902655 None:intergenic 40.0%
ACCCTTATCCAAATGATCCT+CGG - chr4.4:18901156-18901175 MS.gene35190:CDS 40.0%
AGAATAAAGACATGGCAAGG+TGG - chr4.4:18903216-18903235 MS.gene35190:intron 40.0%
AGGAAATGGAGATCAATGGT+GGG - chr4.4:18901709-18901728 MS.gene35190:CDS 40.0%
AGGCAGATAGCAGAAATCAA+TGG - chr4.4:18901633-18901652 MS.gene35190:intron 40.0%
ATGTAATGTACCTAATCCCG+TGG + chr4.4:18902900-18902919 None:intergenic 40.0%
ATTGCATTTCAATTGGACCC+GGG - chr4.4:18902833-18902852 MS.gene35190:intron 40.0%
CACTACCAAGTGAAACAACA+TGG - chr4.4:18902958-18902977 MS.gene35190:CDS 40.0%
CCAGATGGAAAAACTTTGTC+AGG - chr4.4:18901973-18901992 MS.gene35190:CDS 40.0%
GAAATAGCTGGTGTAACTTG+TGG - chr4.4:18902868-18902887 MS.gene35190:CDS 40.0%
GATTGCATTTCAATTGGACC+CGG - chr4.4:18902832-18902851 MS.gene35190:intron 40.0%
GCCATTTGAAGGTGAATTGT+TGG - chr4.4:18903485-18903504 MS.gene35190:CDS 40.0%
GGCAGCCATTAAATATCATG+TGG + chr4.4:18902566-18902585 None:intergenic 40.0%
TCATTTGGATAAGGGTCAGA+AGG + chr4.4:18901152-18901171 None:intergenic 40.0%
TTCCATGCTTTCTTGAATGC+AGG + chr4.4:18901254-18901273 None:intergenic 40.0%
TTGTACAGCATAGGAAGTCT+TGG - chr4.4:18903117-18903136 MS.gene35190:intron 40.0%
! ATTTCCACATTTGAAGTCCC+GGG + chr4.4:18902853-18902872 None:intergenic 40.0%
! CCTGACAAAGTTTTTCCATC+TGG + chr4.4:18901976-18901995 None:intergenic 40.0%
! GAAGAGTTGGAAGGATTTTC+TGG + chr4.4:18901119-18901138 None:intergenic 40.0%
! TCATTTGGAGCAATTGGAGA+TGG - chr4.4:18901212-18901231 MS.gene35190:CDS 40.0%
! TGTTGGATGACCCTTTTTGT+TGG - chr4.4:18903502-18903521 MS.gene35190:CDS 40.0%
!! GCAAACCATGTTGTTTCACT+TGG + chr4.4:18902966-18902985 None:intergenic 40.0%
!! GCTAAGTTTTGGTGCAGAAA+TGG + chr4.4:18902613-18902632 None:intergenic 40.0%
!! GTTGAGCTTTTGCCATTTGA+AGG - chr4.4:18903474-18903493 MS.gene35190:CDS 40.0%
!! TGAATGGTTTTACTCACAGC+TGG + chr4.4:18902012-18902031 None:intergenic 40.0%
!!! TCTTTTGCTTGGTGTGAAAG+TGG + chr4.4:18901058-18901077 None:intergenic 40.0%
AAACTTAGCCCCAACACTGA+TGG - chr4.4:18902624-18902643 MS.gene35190:intron 45.0%
AAAGACATGGCAAGGTGGAA+TGG - chr4.4:18903221-18903240 MS.gene35190:intron 45.0%
AAAGATCACCCACAGTCTCA+GGG + chr4.4:18903590-18903609 None:intergenic 45.0%
AAGACATGGCAAGGTGGAAT+GGG - chr4.4:18903222-18903241 MS.gene35190:intron 45.0%
ACACTAGGAGAAGAGTTGGA+AGG + chr4.4:18901128-18901147 None:intergenic 45.0%
AGGAACACTAGGAGAAGAGT+TGG + chr4.4:18901132-18901151 None:intergenic 45.0%
CACCTGCATTCAAGAAAGCA+TGG - chr4.4:18901249-18901268 MS.gene35190:CDS 45.0%
CATGGATTCCATCAGTGTTG+GGG + chr4.4:18902635-18902654 None:intergenic 45.0%
CCCGAGGATCATTTGGATAA+GGG + chr4.4:18901160-18901179 None:intergenic 45.0%
CCCTTATCCAAATGATCCTC+GGG - chr4.4:18901157-18901176 MS.gene35190:CDS 45.0%
TAAGGGTCAGAAGGAACACT+AGG + chr4.4:18901143-18901162 None:intergenic 45.0%
TCAGGTGGATGGTATGCTAA+TGG - chr4.4:18901583-18901602 MS.gene35190:intron 45.0%
TCCCGAGGATCATTTGGATA+AGG + chr4.4:18901161-18901180 None:intergenic 45.0%
TCTTATGACTGTGTCACGGA+CGG + chr4.4:18903178-18903197 None:intergenic 45.0%
TGATTTCTGCTATCTGCCTC+TGG + chr4.4:18901632-18901651 None:intergenic 45.0%
TGTAGAGTCAGGAGTTCTTC+TGG - chr4.4:18901286-18901305 MS.gene35190:CDS 45.0%
TTTACCCTGTGAGGTTTGCA+GGG + chr4.4:18901740-18901759 None:intergenic 45.0%
!! TCAGTGTTGGGGCTAAGTTT+TGG + chr4.4:18902624-18902643 None:intergenic 45.0%
!!! TTTTCAGGTCCATGCAAACC+AGG - chr4.4:18901347-18901366 MS.gene35190:CDS 45.0%
ATCTTCCCTGCAAACCTCAC+AGG - chr4.4:18901732-18901751 MS.gene35190:CDS 50.0%
ATGTACCTAATCCCGTGGCT+AGG + chr4.4:18902895-18902914 None:intergenic 50.0%
CACCAGATGGACCTGATTCA+TGG - chr4.4:18901606-18901625 MS.gene35190:intron 50.0%
GAAAGATCACCCACAGTCTC+AGG + chr4.4:18903591-18903610 None:intergenic 50.0%
GACGAATCCCGAGGATCATT+TGG + chr4.4:18901167-18901186 None:intergenic 50.0%
GGTATGCTAATGGCACCAGA+TGG - chr4.4:18901593-18901612 MS.gene35190:intron 50.0%
GTTTACCCTGTGAGGTTTGC+AGG + chr4.4:18901741-18901760 None:intergenic 50.0%
TCTTCCCTGCAAACCTCACA+GGG - chr4.4:18901733-18901752 MS.gene35190:CDS 50.0%
TGATCCTCGGGATTCGTCTT+CGG - chr4.4:18901169-18901188 MS.gene35190:CDS 50.0%
! GTAACTTGTGGTCCTAGCCA+CGG - chr4.4:18902880-18902899 MS.gene35190:CDS 50.0%
! TAACTTGTGGTCCTAGCCAC+GGG - chr4.4:18902881-18902900 MS.gene35190:CDS 50.0%
!! TGGCATTTTCAGGGTCCAGA+TGG - chr4.4:18901958-18901977 MS.gene35190:CDS 50.0%
CTCACAGCTGGACTTCCACA+TGG + chr4.4:18902000-18902019 None:intergenic 55.0%
GAAGGTGACCCTGAGACTGT+GGG - chr4.4:18903579-18903598 MS.gene35190:CDS 55.0%
GCAGCTTGTGCTGTAGAGTC+AGG - chr4.4:18901275-18901294 MS.gene35190:CDS 55.0%
GGCCATGAATCAGGTCCATC+TGG + chr4.4:18901611-18901630 None:intergenic 55.0%
TCTGCCTCTGGCCATGAATC+AGG + chr4.4:18901620-18901639 None:intergenic 55.0%
TGGACCCGGGACTTCAAATG+TGG - chr4.4:18902846-18902865 MS.gene35190:intron 55.0%
TGGACCTGATTCATGGCCAG+AGG - chr4.4:18901613-18901632 MS.gene35190:intron 55.0%
CAGTCCGAAGACGAATCCCG+AGG + chr4.4:18901176-18901195 None:intergenic 60.0%
GGAAGGTGACCCTGAGACTG+TGG - chr4.4:18903578-18903597 MS.gene35190:CDS 60.0%
GTGGTCCTAGCCACGGGATT+AGG - chr4.4:18902887-18902906 MS.gene35190:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 18900978 18903632 18900978 ID=MS.gene35190
chr4.4 mRNA 18900978 18903632 18900978 ID=MS.gene35190.t1;Parent=MS.gene35190
chr4.4 exon 18903228 18903632 18903228 ID=MS.gene35190.t1.exon1;Parent=MS.gene35190.t1
chr4.4 CDS 18903228 18903632 18903228 ID=cds.MS.gene35190.t1;Parent=MS.gene35190.t1
chr4.4 exon 18902856 18903023 18902856 ID=MS.gene35190.t1.exon2;Parent=MS.gene35190.t1
chr4.4 CDS 18902856 18903023 18902856 ID=cds.MS.gene35190.t1;Parent=MS.gene35190.t1
chr4.4 exon 18902596 18902640 18902596 ID=MS.gene35190.t1.exon3;Parent=MS.gene35190.t1
chr4.4 CDS 18902596 18902640 18902596 ID=cds.MS.gene35190.t1;Parent=MS.gene35190.t1
chr4.4 exon 18901908 18902115 18901908 ID=MS.gene35190.t1.exon4;Parent=MS.gene35190.t1
chr4.4 CDS 18901908 18902115 18901908 ID=cds.MS.gene35190.t1;Parent=MS.gene35190.t1
chr4.4 exon 18901702 18901783 18901702 ID=MS.gene35190.t1.exon5;Parent=MS.gene35190.t1
chr4.4 CDS 18901702 18901783 18901702 ID=cds.MS.gene35190.t1;Parent=MS.gene35190.t1
chr4.4 exon 18900978 18901485 18900978 ID=MS.gene35190.t1.exon6;Parent=MS.gene35190.t1
chr4.4 CDS 18900978 18901485 18900978 ID=cds.MS.gene35190.t1;Parent=MS.gene35190.t1
Gene Sequence

>MS.gene35190

ATGGATCTCATTCATGGACTATTTATTCTTTGGATTACCTTTATAATATTTAATTTGAGAAATGTGGATGGAAGGAACCACTTTCACACCAAGCAAAAGAAAGTATCTTCCCTTGTTCCTTCTAAGTCTTCAAATGATCCAGAAAATCCTTCCAACTCTTCTCCTAGTGTTCCTTCTGACCCTTATCCAAATGATCCTCGGGATTCGTCTTCGGACTGTATTTTCGATGTTACATCATTTGGAGCAATTGGAGATGGTTCAGCTGATGACACACCTGCATTCAAGAAAGCATGGAAAGCAGCTTGTGCTGTAGAGTCAGGAGTTCTTCTGGTTCCAGAAAATTACACTTTTATGATCACTTCAATTATTTTTTCAGGTCCATGCAAACCAGGATTAGTATTTCAAGTAAGTAAGATAACTTTTTCAATTTCTAATTTCACCCTATATATCATTTATTTTGTTACAAACAAATTATTATTTCTTAAATGCATAAATATCAACTTAATAAAATATGATTGGACGTATGTGTAAAACTGGTTTAACGTTAGTGCGAAATATTCTTACTATTATAATAATATGAAATTATTAACAAAATTGAAATGTAATCAGGTGGATGGTATGCTAATGGCACCAGATGGACCTGATTCATGGCCAGAGGCAGATAGCAGAAATCAATGGCTTGTGTTCTATAAACTTGACCAAATGAGTTTTAATGGTACAGGAACCATTGAAGGAAATGGAGATCAATGGTGGGATCTTCCCTGCAAACCTCACAGGGTAAACTTTTTTGAACATTATTCAAGCATATCTCACCATGTAAATATTTATTTATAGAGATATTTTAAAAACTAATTTAAGAAAGTGACATTATAGTGTAATTTGTTTTACACGGTGAATGTGTACAAATTAATTTCTATTACTATTGTTGTATATATGCATCTAATGTTGATTGATACACACCACTAACCAAAAATAAAACTTGGCATTTTCAGGGTCCAGATGGAAAAACTTTGTCAGGACCATGTGGAAGTCCAGCTGTGAGTAAAACCATTCAGCTCAGTCATTAAATAACATTCTATATGTAATTAATATAGGGTGAAATAAATTTTTTTTATCGTAAAAATATAGAAAATTTTGATTATATAGTCCATAAAAATAAAAACGAGATGTTTTCTTCCTATCAAAATTTTCCATTTTGTTTTTAGTCCCTAATGACTGATTTATGTTTATGCTAATGTCAACATAATTATGATCATTTTTATGTTTCAGATGTATTCAATGTATCTCTACCCCCTGAGTCCATGTTATATTAAAGAAATGTCAAAAACATGTCAAAATTATATCAATTAGGGACAAAAAATGAAACAAAAATTTTCGAGGGACAAAAATATCCTATTTTTATTTTTAAGGACTAAAATTAAAATTCGTTTTATTTTTAGGGGAAGAAAAACTTATTTAATTCAATAATATATTACTAGCTAGTGTCTTAAAGTTGTTTTTAATTTTCATATCTATAGATGATACGGTTCTTCATGAGCTCCAATTTGTATGTGAATGGACTAAAAATTCAAAACAGTCCTCAGTTCCACATGATATTTAATGGCTGCCAAGGAGTAATGATCAATATGTTATCCATTTCTGCACCAAAACTTAGCCCCAACACTGATGGAATCCATGTTGAAAACTCACAAGATGTTGGGATATACAACTCCTTAATAAGCAATGGTAATTAATGGCTATAATCCAATATTTACTTACCCTGTTTGATACTTTGTTTCTATGTTAATTTTATTAATTTTACCATTTTTATGTAAGCAAGCAGCTTAATATAATAACATGATTTGAACAGGTGATGATTGCATTTCAATTGGACCCGGGACTTCAAATGTGGAAATAGCTGGTGTAACTTGTGGTCCTAGCCACGGGATTAGGTACATTACATAATCTATTCTTATTTTGTTGATTAATAAAAATTAGTGTCACTACCAAGTGAAACAACATGGTTTGCTATAATACCAGATCCTAATTTTTCTATTCAAAATATTAAACTATTGACAAAATTTTGATAGTCAATTTTATTTTTTCTAGTAGTACATGCACATATCAGCATGAGTCACTAAACTTAATGTTTTGTTAATTTTGTACAGCATAGGAAGTCTTGGTGTGCACAATTCCCAAGCATGTGTCTCAAACTTAACCGTCCGTGACACAGTCATAAGAGAATCAGACAATGGACTAAGAATAAAGACATGGCAAGGTGGAATGGGATCTGTGTCAAATTTAAAATTTGAGAACATACAAATGGAGAATGTAGGAAACTGCATCCTTATAGACCAATACTATTGTTTGACAAAGGAATGTCTAAATCAAACATCAGCAGTACATGTTAATGATGTGTCCTACAAAAACATTAAGGGTACATATGATGTTAGAACTGCTCCTATTCATTTTGCTTGTAGTGACACTGTTGCTTGTACAAACATAACACTCTCTGAGGTTGAGCTTTTGCCATTTGAAGGTGAATTGTTGGATGACCCTTTTTGTTGGAATGCTTATGGAGATCAAGAGACTTTGACTATACCTCCAATTAATTGTTTAAGGGAAGGTGACCCTGAGACTGTGGGTGATCTTTCTACATATCAATGTAGTAATTAG
Protein sequence

>MS.gene35190.t1

MDLIHGLFILWITFIIFNLRNVDGRNHFHTKQKKVSSLVPSKSSNDPENPSNSSPSVPSDPYPNDPRDSSSDCIFDVTSFGAIGDGSADDTPAFKKAWKAACAVESGVLLVPENYTFMITSIIFSGPCKPGLVFQVDGMLMAPDGPDSWPEADSRNQWLVFYKLDQMSFNGTGTIEGNGDQWWDLPCKPHRGPDGKTLSGPCGSPAMIRFFMSSNLYVNGLKIQNSPQFHMIFNGCQGVMINMLSISAPKLSPNTDGIHVENSQDVGIYNSLISNGDDCISIGPGTSNVEIAGVTCGPSHGISIGSLGVHNSQACVSNLTVRDTVIRESDNGLRIKTWQGGMGSVSNLKFENIQMENVGNCILIDQYYCLTKECLNQTSAVHVNDVSYKNIKGTYDVRTAPIHFACSDTVACTNITLSEVELLPFEGELLDDPFCWNAYGDQETLTIPPINCLREGDPETVGDLSTYQCSN