Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35204.t1 | XP_013457395.1 | 92.7 | 331 | 24 | 0 | 1 | 331 | 1 | 331 | 4.20E-177 | 630.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35204.t1 | Q3TUH1 | 32.6 | 319 | 199 | 6 | 18 | 330 | 24 | 332 | 1.5e-42 | 174.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35204.t1 | G8A1V5 | 92.7 | 331 | 24 | 0 | 1 | 331 | 1 | 331 | 3.0e-177 | 630.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049824 | MS.gene35204 | 0.811043 | 8.53E-51 | -1.69E-46 |
| MS.gene056091 | MS.gene35204 | 0.813864 | 2.06E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35204.t1 | MTR_4g094760 | 92.749 | 331 | 24 | 0 | 1 | 331 | 1 | 331 | 0.0 | 622 |
| MS.gene35204.t1 | MTR_4g094760 | 92.517 | 294 | 22 | 0 | 38 | 331 | 1 | 294 | 0.0 | 545 |
| MS.gene35204.t1 | MTR_4g094760 | 92.520 | 254 | 19 | 0 | 78 | 331 | 10 | 263 | 2.51e-165 | 461 |
| MS.gene35204.t1 | MTR_4g094760 | 93.562 | 233 | 15 | 0 | 1 | 233 | 1 | 233 | 7.69e-157 | 439 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35204.t1 | AT3G47630 | 65.741 | 324 | 111 | 0 | 6 | 329 | 7 | 330 | 1.97e-156 | 441 |
| MS.gene35204.t1 | AT3G47630 | 65.741 | 324 | 111 | 0 | 6 | 329 | 7 | 330 | 1.97e-156 | 441 |
| MS.gene35204.t1 | AT3G47630 | 65.741 | 324 | 111 | 0 | 6 | 329 | 7 | 330 | 1.97e-156 | 441 |
| MS.gene35204.t1 | AT3G47630 | 65.763 | 295 | 101 | 0 | 35 | 329 | 24 | 318 | 2.41e-138 | 395 |
| MS.gene35204.t1 | AT3G47630 | 66.045 | 268 | 91 | 0 | 62 | 329 | 6 | 273 | 1.13e-123 | 356 |
| MS.gene35204.t1 | AT3G47630 | 62.780 | 223 | 83 | 0 | 107 | 329 | 1 | 223 | 3.71e-92 | 274 |
Find 65 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTGAGTTTGCTTGCGTTTA+TGG | 0.096576 | 4.4:-18739426 | MS.gene35204:CDS |
| TCAAATTGTTGTTGATAATT+TGG | 0.122916 | 4.4:-18734898 | MS.gene35204:CDS |
| ACCATGATTGATTATATTCT+TGG | 0.194064 | 4.4:-18739287 | MS.gene35204:CDS |
| CTCCTTGCTTCATGCTCAAT+TGG | 0.235122 | 4.4:+18732924 | None:intergenic |
| AATTTGTAATCTCTCATATA+TGG | 0.240141 | 4.4:-18734697 | MS.gene35204:CDS |
| AGACCTGAGATACTTGTATT+TGG | 0.288139 | 4.4:+18734056 | None:intergenic |
| ACCTCAGAATGCCATTGTTT+GGG | 0.293822 | 4.4:+18739254 | None:intergenic |
| TATATGCAGATGTTCAAGTA+TGG | 0.294312 | 4.4:-18738683 | MS.gene35204:intron |
| ACCGCGGTTGCAGATAAAAT+TGG | 0.296872 | 4.4:-18738930 | MS.gene35204:CDS |
| CTCAATTGGCTTCTGATTGA+TGG | 0.298730 | 4.4:+18732938 | None:intergenic |
| ACTTCAAGATGTGCAGTATT+GGG | 0.344897 | 4.4:-18738639 | MS.gene35204:CDS |
| CAGGTGAAGAAGATTGTAAC+TGG | 0.350465 | 4.4:-18734169 | MS.gene35204:CDS |
| TACCTCAGAATGCCATTGTT+TGG | 0.352393 | 4.4:+18739253 | None:intergenic |
| AGTGCAAGGCAGGCCGTATC+TGG | 0.358772 | 4.4:-18732567 | MS.gene35204:CDS |
| GCCTCGTATTCTTCAAGAAA+TGG | 0.368181 | 4.4:+18734115 | None:intergenic |
| TAAACGCAAGCAAACTCAAC+AGG | 0.400684 | 4.4:+18739429 | None:intergenic |
| ATTTGGTGATTGACTGTTGA+TGG | 0.403147 | 4.4:+18734073 | None:intergenic |
| TACTTCAAGATGTGCAGTAT+TGG | 0.416483 | 4.4:-18738640 | MS.gene35204:CDS |
| CTGGCCTACTGGCTGCTGGT+GGG | 0.456512 | 4.4:-18732548 | MS.gene35204:CDS |
| GTATCTGGCCTACTGGCTGC+TGG | 0.457813 | 4.4:-18732552 | MS.gene35204:CDS |
| GACGTGAAATCGAGTCGAAC+TGG | 0.464437 | 4.4:-18732899 | MS.gene35204:intron |
| ATTTGTAATCTCTCATATAT+GGG | 0.464476 | 4.4:-18734696 | MS.gene35204:CDS |
| GCAGGCCGTATCTGGCCTAC+TGG | 0.467270 | 4.4:-18732559 | MS.gene35204:CDS |
| TCAATAAAGCTTGGAAGTCA+TGG | 0.467413 | 4.4:-18732494 | MS.gene35204:CDS |
| TTTCCGTTCCACGTAACAAA+AGG | 0.473223 | 4.4:+18738888 | None:intergenic |
| GCACCTTGGCGGAGAGAGGC+TGG | 0.473894 | 4.4:-18739067 | MS.gene35204:intron |
| TCACCAAATACAAGTATCTC+AGG | 0.479485 | 4.4:-18734059 | MS.gene35204:intron |
| TATGGTTTCAAGTGCAAGGC+AGG | 0.490103 | 4.4:-18732577 | MS.gene35204:CDS |
| TAAACCTCTGTGAATTCTGA+TGG | 0.503955 | 4.4:+18734813 | None:intergenic |
| AGCAGCCAGTAGGCCAGATA+CGG | 0.507076 | 4.4:+18732554 | None:intergenic |
| TGGCCTACTGGCTGCTGGTG+GGG | 0.511323 | 4.4:-18732547 | MS.gene35204:CDS |
| ACCAAGAATATAATCAATCA+TGG | 0.514721 | 4.4:+18739286 | None:intergenic |
| CCAACAAAATCAATAAAGCT+TGG | 0.517401 | 4.4:-18732503 | MS.gene35204:CDS |
| TCTGGCCTACTGGCTGCTGG+TGG | 0.520815 | 4.4:-18732549 | MS.gene35204:CDS |
| ATATGCAGATGTTCAAGTAT+GGG | 0.527202 | 4.4:-18738682 | MS.gene35204:intron |
| AAAGAAGACCTTGTCATGGT+TGG | 0.546678 | 4.4:+18739386 | None:intergenic |
| ACAAGCATCACTATGCATCA+TGG | 0.560182 | 4.4:-18739095 | MS.gene35204:CDS |
| GGGTTATGGTTTCAAGTGCA+AGG | 0.565532 | 4.4:-18732581 | MS.gene35204:CDS |
| TCATGGATGGTGCACCTTGG+CGG | 0.566477 | 4.4:-18739078 | MS.gene35204:CDS |
| TGGTGCACCTTGGCGGAGAG+AGG | 0.568336 | 4.4:-18739071 | MS.gene35204:CDS |
| AAGACCTTGTCATGGTTGGT+TGG | 0.581679 | 4.4:+18739390 | None:intergenic |
| GTACCAGCCTCTCTCCGCCA+AGG | 0.582816 | 4.4:+18739064 | None:intergenic |
| TGGGATAAATTCTACTTGTG+TGG | 0.584350 | 4.4:-18738620 | MS.gene35204:CDS |
| AAACGCAAGCAAACTCAACA+GGG | 0.592632 | 4.4:+18739430 | None:intergenic |
| GAAATGGCTTATACGTCGAG+TGG | 0.597222 | 4.4:+18734131 | None:intergenic |
| GTTGCAGATAAAATTGGCGT+GGG | 0.607310 | 4.4:-18738924 | MS.gene35204:CDS |
| GGTTGCAGATAAAATTGGCG+TGG | 0.610241 | 4.4:-18738925 | MS.gene35204:CDS |
| AGCCAATTGAGCATGAAGCA+AGG | 0.610932 | 4.4:-18732926 | MS.gene35204:CDS |
| AATTGGCTTCTGATTGATGG+AGG | 0.612804 | 4.4:+18732941 | None:intergenic |
| GTGTTTCTGACCCCAAACAA+TGG | 0.614293 | 4.4:-18739265 | MS.gene35204:CDS |
| TCATCCAACCAACCATGACA+AGG | 0.620663 | 4.4:-18739394 | MS.gene35204:intron |
| TATGCAGATGTTCAAGTATG+GGG | 0.622561 | 4.4:-18738681 | MS.gene35204:intron |
| CCTCAGAATGCCATTGTTTG+GGG | 0.623734 | 4.4:+18739255 | None:intergenic |
| GCATCACTATGCATCATGGA+TGG | 0.628377 | 4.4:-18739091 | MS.gene35204:CDS |
| TTACCCCACCAGCAGCCAGT+AGG | 0.636171 | 4.4:+18732544 | None:intergenic |
| CCCCAAACAATGGCATTCTG+AGG | 0.637750 | 4.4:-18739255 | MS.gene35204:intron |
| AACAAAAGAAGACCTTGTCA+TGG | 0.645360 | 4.4:+18739382 | None:intergenic |
| TGTGCGCATGTTATTTGCTG+AGG | 0.646332 | 4.4:-18734670 | MS.gene35204:CDS |
| GCATCATGGATGGTGCACCT+TGG | 0.647771 | 4.4:-18739081 | MS.gene35204:CDS |
| GCCATTTCTTGAAGAATACG+AGG | 0.652695 | 4.4:-18734116 | MS.gene35204:CDS |
| AGGTGAAGAAGATTGTAACT+GGG | 0.678071 | 4.4:-18734168 | MS.gene35204:CDS |
| ACGCAAGCAAACTCAACAGG+GGG | 0.689789 | 4.4:+18739432 | None:intergenic |
| AACGCAAGCAAACTCAACAG+GGG | 0.716547 | 4.4:+18739431 | None:intergenic |
| TGTAACATGTAAGATTACCG+CGG | 0.723354 | 4.4:-18738946 | MS.gene35204:intron |
| GCTTCCATCAGAATTCACAG+AGG | 0.776503 | 4.4:-18734817 | MS.gene35204:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 18732488 | 18739499 | 18732488 | ID=MS.gene35204 |
| chr4.4 | mRNA | 18732488 | 18739499 | 18732488 | ID=MS.gene35204.t1;Parent=MS.gene35204 |
| chr4.4 | exon | 18739395 | 18739499 | 18739395 | ID=MS.gene35204.t1.exon1;Parent=MS.gene35204.t1 |
| chr4.4 | CDS | 18739395 | 18739499 | 18739395 | ID=cds.MS.gene35204.t1;Parent=MS.gene35204.t1 |
| chr4.4 | exon | 18739256 | 18739312 | 18739256 | ID=MS.gene35204.t1.exon2;Parent=MS.gene35204.t1 |
| chr4.4 | CDS | 18739256 | 18739312 | 18739256 | ID=cds.MS.gene35204.t1;Parent=MS.gene35204.t1 |
| chr4.4 | exon | 18739068 | 18739130 | 18739068 | ID=MS.gene35204.t1.exon3;Parent=MS.gene35204.t1 |
| chr4.4 | CDS | 18739068 | 18739130 | 18739068 | ID=cds.MS.gene35204.t1;Parent=MS.gene35204.t1 |
| chr4.4 | exon | 18738887 | 18738955 | 18738887 | ID=MS.gene35204.t1.exon4;Parent=MS.gene35204.t1 |
| chr4.4 | CDS | 18738887 | 18738955 | 18738887 | ID=cds.MS.gene35204.t1;Parent=MS.gene35204.t1 |
| chr4.4 | exon | 18738604 | 18738696 | 18738604 | ID=MS.gene35204.t1.exon5;Parent=MS.gene35204.t1 |
| chr4.4 | CDS | 18738604 | 18738696 | 18738604 | ID=cds.MS.gene35204.t1;Parent=MS.gene35204.t1 |
| chr4.4 | exon | 18734818 | 18734922 | 18734818 | ID=MS.gene35204.t1.exon6;Parent=MS.gene35204.t1 |
| chr4.4 | CDS | 18734818 | 18734922 | 18734818 | ID=cds.MS.gene35204.t1;Parent=MS.gene35204.t1 |
| chr4.4 | exon | 18734659 | 18734736 | 18734659 | ID=MS.gene35204.t1.exon7;Parent=MS.gene35204.t1 |
| chr4.4 | CDS | 18734659 | 18734736 | 18734659 | ID=cds.MS.gene35204.t1;Parent=MS.gene35204.t1 |
| chr4.4 | exon | 18734060 | 18734188 | 18734060 | ID=MS.gene35204.t1.exon8;Parent=MS.gene35204.t1 |
| chr4.4 | CDS | 18734060 | 18734188 | 18734060 | ID=cds.MS.gene35204.t1;Parent=MS.gene35204.t1 |
| chr4.4 | exon | 18732900 | 18733005 | 18732900 | ID=MS.gene35204.t1.exon9;Parent=MS.gene35204.t1 |
| chr4.4 | CDS | 18732900 | 18733005 | 18732900 | ID=cds.MS.gene35204.t1;Parent=MS.gene35204.t1 |
| chr4.4 | exon | 18732488 | 18732678 | 18732488 | ID=MS.gene35204.t1.exon10;Parent=MS.gene35204.t1 |
| chr4.4 | CDS | 18732488 | 18732678 | 18732488 | ID=cds.MS.gene35204.t1;Parent=MS.gene35204.t1 |
| Gene Sequence |
| Protein sequence |
