AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35299

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35299.t1 MTR_2g084360 90.863 197 17 1 19 215 1 196 2.41e-121 350
MS.gene35299.t1 MTR_2g093960 36.129 155 86 3 224 378 41 182 3.25e-26 109
MS.gene35299.t1 MTR_4g059870 34.839 155 88 3 224 378 47 188 4.51e-25 107
MS.gene35299.t1 MTR_8g017210 35.484 155 86 3 224 378 71 211 4.70e-25 107
MS.gene35299.t1 MTR_1g093095 36.723 177 104 7 224 397 95 266 2.11e-24 102
MS.gene35299.t1 MTR_1g112270 36.129 155 85 4 224 378 53 193 1.35e-23 103
MS.gene35299.t1 MTR_2g099990 34.177 158 90 3 224 381 55 198 2.06e-23 102
MS.gene35299.t1 MTR_1g112270 36.184 152 83 4 227 378 67 204 6.03e-23 101
MS.gene35299.t1 MTR_1g016010 33.548 155 90 3 224 378 50 191 1.36e-22 99.4
MS.gene35299.t1 MTR_1g094115 34.194 155 88 4 224 378 56 196 3.84e-22 98.6
MS.gene35299.t1 MTR_2g090745 33.333 159 90 4 220 378 14 156 1.24e-20 93.6
MS.gene35299.t1 MTR_2g099990 36.029 136 73 3 246 381 8 129 1.59e-20 92.8
MS.gene35299.t1 MTR_1g094115 35.338 133 72 4 246 378 61 179 1.65e-18 87.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35299.t1 AT5G22890 47.486 358 126 7 44 388 53 361 8.28e-110 327
MS.gene35299.t1 AT1G34370 59.639 166 67 0 224 389 14 179 1.45e-73 231
MS.gene35299.t1 AT1G34370 36.082 388 197 9 8 389 47 389 1.88e-73 238
MS.gene35299.t1 AT1G34370 36.082 388 197 9 8 389 47 389 1.88e-73 238
MS.gene35299.t1 AT3G45260 36.129 155 86 3 224 378 48 189 4.70e-25 106
MS.gene35299.t1 AT3G45260 36.129 155 86 3 224 378 48 189 4.70e-25 106
MS.gene35299.t1 AT5G60470 34.839 155 88 4 224 378 53 194 5.83e-25 106
MS.gene35299.t1 AT5G60470 34.839 155 88 4 224 378 49 190 5.92e-25 106
MS.gene35299.t1 AT5G03150 35.484 155 87 3 224 378 62 203 1.96e-24 105
MS.gene35299.t1 AT5G44160 34.194 155 88 3 224 378 46 186 2.53e-24 104
MS.gene35299.t1 AT1G03840 33.548 155 89 3 224 378 50 190 2.75e-24 104
MS.gene35299.t1 AT5G60470 35.333 150 84 4 229 378 49 185 2.90e-24 104
MS.gene35299.t1 AT1G34790 38.012 171 96 6 225 392 124 287 5.71e-24 101
MS.gene35299.t1 AT5G66730 35.484 155 86 4 224 378 41 181 8.81e-24 103
MS.gene35299.t1 AT1G55110 34.839 155 88 3 224 378 72 213 1.06e-23 102
MS.gene35299.t1 AT1G55110 34.839 155 88 3 224 378 72 213 1.06e-23 102
MS.gene35299.t1 AT1G55110 34.839 155 88 3 224 378 72 213 1.06e-23 102
MS.gene35299.t1 AT2G02080 36.774 155 84 4 224 378 63 203 1.20e-23 103
MS.gene35299.t1 AT2G02080 36.774 155 84 4 224 378 63 203 1.20e-23 103
MS.gene35299.t1 AT2G02080 36.774 155 84 4 224 378 63 203 1.20e-23 103
MS.gene35299.t1 AT2G02080 36.774 155 84 4 224 378 63 203 1.20e-23 103
MS.gene35299.t1 AT1G03840 33.553 152 87 3 227 378 51 188 2.44e-23 102
MS.gene35299.t1 AT5G44160 34.211 152 86 3 227 378 10 147 2.52e-23 101
MS.gene35299.t1 AT4G02670 35.484 155 87 3 224 378 62 203 2.98e-23 100
MS.gene35299.t1 AT4G02670 35.484 155 87 3 224 378 64 205 3.04e-23 100
MS.gene35299.t1 AT2G02070 36.129 155 85 4 224 378 61 201 3.43e-23 102
MS.gene35299.t1 AT2G02070 36.129 155 85 4 224 378 61 201 3.43e-23 102
MS.gene35299.t1 AT3G13810 33.548 155 90 3 224 378 74 215 6.91e-23 100
MS.gene35299.t1 AT3G13810 33.548 155 90 3 224 378 79 220 7.41e-23 100
MS.gene35299.t1 AT3G13810 33.548 155 90 3 224 378 79 220 7.41e-23 100
MS.gene35299.t1 AT3G13810 33.548 155 90 3 224 378 60 201 7.86e-23 100
MS.gene35299.t1 AT3G50700 34.839 155 87 4 224 378 43 183 1.12e-22 99.8
MS.gene35299.t1 AT3G13810 33.553 152 88 3 227 378 83 221 5.69e-22 98.2
MS.gene35299.t1 AT1G14580 33.548 155 89 4 224 378 62 202 6.06e-22 97.8
MS.gene35299.t1 AT1G14580 33.548 155 89 4 224 378 62 202 6.06e-22 97.8
MS.gene35299.t1 AT3G13810 33.553 152 88 3 227 378 69 207 6.11e-22 97.8
MS.gene35299.t1 AT1G14580 33.548 155 89 4 224 378 76 216 6.79e-22 97.8
MS.gene35299.t1 AT1G51220 39.161 143 79 4 246 387 180 315 1.22e-21 95.5
MS.gene35299.t1 AT3G20880 39.161 143 79 4 246 387 258 393 1.33e-21 96.3
MS.gene35299.t1 AT1G08290 38.889 144 79 6 246 387 182 318 1.65e-21 95.1
MS.gene35299.t1 AT3G57670 38.298 141 80 3 246 386 219 352 1.68e-21 95.5
MS.gene35299.t1 AT1G13290 39.007 141 81 3 246 386 103 238 2.69e-21 93.6
MS.gene35299.t1 AT2G01940 35.669 157 85 5 224 378 53 195 4.87e-21 94.7
MS.gene35299.t1 AT2G02080 37.594 133 69 4 246 378 8 126 6.71e-20 91.7
MS.gene35299.t1 AT2G02080 37.594 133 69 4 246 378 8 126 6.71e-20 91.7
MS.gene35299.t1 AT1G25250 33.333 156 91 4 224 378 42 185 1.65e-19 89.7
MS.gene35299.t1 AT1G68130 32.692 156 91 4 224 378 50 192 9.86e-19 87.8
MS.gene35299.t1 AT1G25250 33.333 153 89 4 227 378 22 162 1.33e-18 87.0
MS.gene35299.t1 AT2G01940 35.862 145 77 5 224 366 53 183 3.23e-18 86.7

Find 87 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCTTGCATACATACATTT+TGG 0.081024 2.1:+17248438 None:intergenic
GACGATACATGCTTAAAATT+TGG 0.102733 2.1:-17248802 MS.gene35299:CDS
TCTAATATTCAATCAAATTT+TGG 0.121082 2.1:-17249042 MS.gene35299:CDS
AATTTGGGTCATCTTATAAT+TGG 0.155349 2.1:-17248786 MS.gene35299:CDS
TAATCTCTATTCTTAGTAAT+TGG 0.163762 2.1:+17248592 None:intergenic
AGACCCTCTTATACTATTAT+TGG 0.167733 2.1:+17248861 None:intergenic
GAAATTCCTTCCATGGAATT+TGG 0.168179 2.1:+17249336 None:intergenic
ACTATCTCATTGTCCAATTC+TGG 0.197440 2.1:+17248757 None:intergenic
TTGACGAGTCTTGTTGATTT+GGG 0.269721 2.1:+17249217 None:intergenic
TTCTAGGAAAGATAAGCTTA+TGG 0.293207 2.1:-17248320 MS.gene35299:CDS
TTTCTGTTCTTTCTGATTTG+AGG 0.317726 2.1:-17248399 MS.gene35299:CDS
ACGATACATGCTTAAAATTT+GGG 0.323396 2.1:-17248801 MS.gene35299:CDS
ATAATAGTAACATTAATTAT+AGG 0.329713 2.1:-17248933 MS.gene35299:CDS
GAGATTGAAAAGGAGGGAAT+TGG 0.343144 2.1:+17249296 None:intergenic
TCTAGGAAAGATAAGCTTAT+GGG 0.346344 2.1:-17248319 MS.gene35299:CDS
ACACCTCTAGGGTTTGAAAT+AGG 0.353386 2.1:-17249411 MS.gene35299:CDS
GTTGACGAGTCTTGTTGATT+TGG 0.361562 2.1:+17249216 None:intergenic
ACAAGACTCGTCAACATCTA+TGG 0.370241 2.1:-17249208 MS.gene35299:CDS
ATTTGGGTCATCTTATAATT+GGG 0.382161 2.1:-17248785 MS.gene35299:CDS
TAATTGGGTCGGCCCAGAAT+TGG 0.390164 2.1:-17248770 MS.gene35299:CDS
TATGACAAACCATTGATAAC+CGG 0.393477 2.1:+17248268 None:intergenic
GAAACCAATAATAGTATAAG+AGG 0.400835 2.1:-17248865 MS.gene35299:CDS
GTCCTCAAGAAGGGTGTAGA+TGG 0.403741 2.1:-17248537 MS.gene35299:CDS
AGAAACATTGTGGTGATCTT+AGG 0.406285 2.1:-17248369 MS.gene35299:CDS
AATCAAGTGCAAACTCTTGT+TGG 0.406380 2.1:-17249252 MS.gene35299:CDS
AAGCTTATCTTTCCTAGAAA+AGG 0.414705 2.1:+17248324 None:intergenic
ACACATTATTGTCAAGTTTG+TGG 0.417742 2.1:-17248700 MS.gene35299:CDS
GGACATGTTGGTTTGTTTGT+GGG 0.428197 2.1:-17248298 MS.gene35299:CDS
CTATCTCATTGTCCAATTCT+GGG 0.439489 2.1:+17248758 None:intergenic
GGGACATGTTGGTTTGTTTG+TGG 0.441560 2.1:-17248299 MS.gene35299:CDS
TACAATGATTTCTTGTATTG+TGG 0.444279 2.1:+17249167 None:intergenic
TGTGTACTTGGCTAATAAAT+CGG 0.444661 2.1:+17248720 None:intergenic
ATCAAATCTTCCGTGATCAT+AGG 0.450294 2.1:+17249366 None:intergenic
TAATAATGAGTCAGAACCAT+TGG 0.457675 2.1:-17248974 MS.gene35299:CDS
GAGTAGAGAGATTGAAAAGG+AGG 0.459196 2.1:+17249289 None:intergenic
TATTGTCAAGTTTGTGGCAA+AGG 0.467134 2.1:-17248694 MS.gene35299:CDS
TCAGAACCATTGGATTGGTT+TGG 0.473677 2.1:-17248964 MS.gene35299:CDS
ATTGTCAAGTTTGTGGCAAA+GGG 0.476447 2.1:-17248693 MS.gene35299:CDS
ATTGAAAAGGAGGGAATTGG+AGG 0.476928 2.1:+17249299 None:intergenic
TGAACTAGAAATTCCTTCCA+TGG 0.477549 2.1:+17249329 None:intergenic
GGACATCACCCGGTTATCAA+TGG 0.481764 2.1:-17248277 MS.gene35299:CDS
GACACAAACATTGATCTTAG+AGG 0.484429 2.1:-17249012 MS.gene35299:CDS
GATGTCATTGCCAAATTCCA+TGG 0.493187 2.1:-17249346 MS.gene35299:CDS
TAATAGAAGTAGTACCTTGA+GGG 0.496518 2.1:+17249097 None:intergenic
TGAGAGTAGAGAGATTGAAA+AGG 0.497992 2.1:+17249286 None:intergenic
AGGATGATATTGATGATCAA+AGG 0.500011 2.1:-17248906 MS.gene35299:CDS
ATTTCAAGGACAACACCTCT+AGG 0.500290 2.1:-17249423 MS.gene35299:CDS
AGGATGCATATGAGAGCTCA+TGG 0.501614 2.1:-17248649 MS.gene35299:CDS
GATAAGCTTATGGGACATGT+TGG 0.504366 2.1:-17248310 MS.gene35299:CDS
TTTGTTTGTGGGACATCACC+CGG 0.508232 2.1:-17248287 MS.gene35299:CDS
TGATCATAGGAAACATTTGT+AGG 0.513435 2.1:+17249379 None:intergenic
TGACTTCAAAGGTTGAAACT+TGG 0.516503 2.1:+17248501 None:intergenic
ATGAGTCAGAACCATTGGAT+TGG 0.516915 2.1:-17248969 MS.gene35299:CDS
GCACAAATCAATGACTTCAA+AGG 0.522700 2.1:+17248490 None:intergenic
AAATATTCATGTCCTCAAGA+AGG 0.523706 2.1:-17248547 MS.gene35299:CDS
AGTAGAGAGATTGAAAAGGA+GGG 0.529561 2.1:+17249290 None:intergenic
GGATGATATTGATGATCAAA+GGG 0.531710 2.1:-17248905 MS.gene35299:CDS
TTGACAATAATGTGTGTACT+TGG 0.534066 2.1:+17248708 None:intergenic
GGGTTTGAAATAGGAAATCA+AGG 0.537141 2.1:-17249402 MS.gene35299:CDS
GGGTCATCTTATAATTGGGT+CGG 0.542248 2.1:-17248781 MS.gene35299:CDS
TCAAGAGGGATGCAAATTTG+AGG 0.543405 2.1:-17248669 MS.gene35299:CDS
AATATTCATGTCCTCAAGAA+GGG 0.556447 2.1:-17248546 MS.gene35299:CDS
TTGTATTGTGGAGTTCATTG+TGG 0.558473 2.1:+17249179 None:intergenic
TAACATTAATTATAGGCTCA+AGG 0.571276 2.1:-17248926 MS.gene35299:CDS
TTCCATCTACACCCTTCTTG+AGG 0.572019 2.1:+17248535 None:intergenic
TTTCAAGGACAACACCTCTA+GGG 0.577368 2.1:-17249422 MS.gene35299:CDS
GGAAACTAAGAGCAATCTGT+TGG 0.579939 2.1:+17249118 None:intergenic
CTTTCACCAAACCAATCCAA+TGG 0.587496 2.1:+17248958 None:intergenic
TTTGTGGCAAAGGGTTCAAG+AGG 0.595190 2.1:-17248684 MS.gene35299:CDS
TCATTGCCAAATTCCATGGA+AGG 0.596577 2.1:-17249342 MS.gene35299:CDS
ATGACAAACCATTGATAACC+GGG 0.597673 2.1:+17248269 None:intergenic
ACAAATGTTTCCTATGATCA+CGG 0.598164 2.1:-17249376 MS.gene35299:CDS
CTTAGGTGGCAGTGTTCATG+TGG 0.605201 2.1:-17248352 MS.gene35299:CDS
AACATTGTGGTGATCTTAGG+TGG 0.610982 2.1:-17248366 MS.gene35299:CDS
TGACGAGTCTTGTTGATTTG+GGG 0.614111 2.1:+17249218 None:intergenic
ATTGCTCTTAGTTTCCCTCA+AGG 0.618106 2.1:-17249111 MS.gene35299:CDS
TTAATAGAAGTAGTACCTTG+AGG 0.620130 2.1:+17249096 None:intergenic
AAACCAATAATAGTATAAGA+GGG 0.620427 2.1:-17248864 MS.gene35299:CDS
TTGAAAAGGAGGGAATTGGA+GGG 0.623406 2.1:+17249300 None:intergenic
GGATGCATATGAGAGCTCAT+GGG 0.624368 2.1:-17248648 MS.gene35299:CDS
GCAAGTGAACATCATTGATG+TGG 0.636466 2.1:+17249140 None:intergenic
CCAAAATGTATGTATGCAAG+AGG 0.650780 2.1:-17248438 MS.gene35299:CDS
TTTCCTATTTCAAACCCTAG+AGG 0.660481 2.1:+17249408 None:intergenic
GATGCATATGAGAGCTCATG+GGG 0.685805 2.1:-17248647 MS.gene35299:CDS
TTGTGGCAAAGGGTTCAAGA+GGG 0.697728 2.1:-17248683 MS.gene35299:CDS
AGGACACATGAGAAACATTG+TGG 0.713628 2.1:-17248379 MS.gene35299:CDS
TGAGATAGTTGAATTAGACG+CGG 0.752880 2.1:-17248743 MS.gene35299:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATAGTAACATTAATTAT+AGG - chr2.1:17248742-17248761 MS.gene35299:CDS 10.0%
!!! TCTAATATTCAATCAAATTT+TGG - chr2.1:17248633-17248652 MS.gene35299:CDS 15.0%
!! AAACCAATAATAGTATAAGA+GGG - chr2.1:17248811-17248830 MS.gene35299:CDS 20.0%
!! TAATCTCTATTCTTAGTAAT+TGG + chr2.1:17249086-17249105 None:intergenic 20.0%
!!! ATTTTTGCTTTTGAAATTCT+TGG + chr2.1:17248614-17248633 None:intergenic 20.0%
! AATTTGGGTCATCTTATAAT+TGG - chr2.1:17248889-17248908 MS.gene35299:CDS 25.0%
! ACGATACATGCTTAAAATTT+GGG - chr2.1:17248874-17248893 MS.gene35299:CDS 25.0%
! ATTTGGGTCATCTTATAATT+GGG - chr2.1:17248890-17248909 MS.gene35299:CDS 25.0%
! GAAACCAATAATAGTATAAG+AGG - chr2.1:17248810-17248829 MS.gene35299:CDS 25.0%
! TAACATTAATTATAGGCTCA+AGG - chr2.1:17248749-17248768 MS.gene35299:CDS 25.0%
! TACAATGATTTCTTGTATTG+TGG + chr2.1:17248511-17248530 None:intergenic 25.0%
AAATATTCATGTCCTCAAGA+AGG - chr2.1:17249128-17249147 MS.gene35299:CDS 30.0%
AAGCTTATCTTTCCTAGAAA+AGG + chr2.1:17249354-17249373 None:intergenic 30.0%
AATATTCATGTCCTCAAGAA+GGG - chr2.1:17249129-17249148 MS.gene35299:CDS 30.0%
ACAAATGTTTCCTATGATCA+CGG - chr2.1:17248299-17248318 MS.gene35299:CDS 30.0%
ACACATTATTGTCAAGTTTG+TGG - chr2.1:17248975-17248994 MS.gene35299:CDS 30.0%
AGACCCTCTTATACTATTAT+TGG + chr2.1:17248817-17248836 None:intergenic 30.0%
GACGATACATGCTTAAAATT+TGG - chr2.1:17248873-17248892 MS.gene35299:CDS 30.0%
TAATAATGAGTCAGAACCAT+TGG - chr2.1:17248701-17248720 MS.gene35299:CDS 30.0%
TAATAGAAGTAGTACCTTGA+GGG + chr2.1:17248581-17248600 None:intergenic 30.0%
TCTAGGAAAGATAAGCTTAT+GGG - chr2.1:17249356-17249375 MS.gene35299:CDS 30.0%
TGATCATAGGAAACATTTGT+AGG + chr2.1:17248299-17248318 None:intergenic 30.0%
TGTGTACTTGGCTAATAAAT+CGG + chr2.1:17248958-17248977 None:intergenic 30.0%
TTAATAGAAGTAGTACCTTG+AGG + chr2.1:17248582-17248601 None:intergenic 30.0%
TTGACAATAATGTGTGTACT+TGG + chr2.1:17248970-17248989 None:intergenic 30.0%
! AGGATGATATTGATGATCAA+AGG - chr2.1:17248769-17248788 MS.gene35299:CDS 30.0%
! CTCTTGCATACATACATTTT+GGG + chr2.1:17249239-17249258 None:intergenic 30.0%
! GGATGATATTGATGATCAAA+GGG - chr2.1:17248770-17248789 MS.gene35299:CDS 30.0%
! TATGACAAACCATTGATAAC+CGG + chr2.1:17249410-17249429 None:intergenic 30.0%
! TTCTAGGAAAGATAAGCTTA+TGG - chr2.1:17249355-17249374 MS.gene35299:CDS 30.0%
! TTTCTGTTCTTTCTGATTTG+AGG - chr2.1:17249276-17249295 MS.gene35299:CDS 30.0%
AATCAAGTGCAAACTCTTGT+TGG - chr2.1:17248423-17248442 MS.gene35299:CDS 35.0%
ACTATCTCATTGTCCAATTC+TGG + chr2.1:17248921-17248940 None:intergenic 35.0%
AGAAACATTGTGGTGATCTT+AGG - chr2.1:17249306-17249325 MS.gene35299:CDS 35.0%
AGTAGAGAGATTGAAAAGGA+GGG + chr2.1:17248388-17248407 None:intergenic 35.0%
ATCAAATCTTCCGTGATCAT+AGG + chr2.1:17248312-17248331 None:intergenic 35.0%
ATTGTCAAGTTTGTGGCAAA+GGG - chr2.1:17248982-17249001 MS.gene35299:CDS 35.0%
CCAAAATGTATGTATGCAAG+AGG - chr2.1:17249237-17249256 MS.gene35299:CDS 35.0%
CTATCTCATTGTCCAATTCT+GGG + chr2.1:17248920-17248939 None:intergenic 35.0%
GAAATTCCTTCCATGGAATT+TGG + chr2.1:17248342-17248361 None:intergenic 35.0%
GACACAAACATTGATCTTAG+AGG - chr2.1:17248663-17248682 MS.gene35299:CDS 35.0%
GCACAAATCAATGACTTCAA+AGG + chr2.1:17249188-17249207 None:intergenic 35.0%
TATTGTCAAGTTTGTGGCAA+AGG - chr2.1:17248981-17249000 MS.gene35299:CDS 35.0%
TGAACTAGAAATTCCTTCCA+TGG + chr2.1:17248349-17248368 None:intergenic 35.0%
TGACTTCAAAGGTTGAAACT+TGG + chr2.1:17249177-17249196 None:intergenic 35.0%
TGAGAGTAGAGAGATTGAAA+AGG + chr2.1:17248392-17248411 None:intergenic 35.0%
TGAGATAGTTGAATTAGACG+CGG - chr2.1:17248932-17248951 MS.gene35299:CDS 35.0%
TTGTATTGTGGAGTTCATTG+TGG + chr2.1:17248499-17248518 None:intergenic 35.0%
TTTCCTATTTCAAACCCTAG+AGG + chr2.1:17248270-17248289 None:intergenic 35.0%
! ATGACAAACCATTGATAACC+GGG + chr2.1:17249409-17249428 None:intergenic 35.0%
! CCTCTTGCATACATACATTT+TGG + chr2.1:17249240-17249259 None:intergenic 35.0%
! GGGTTTGAAATAGGAAATCA+AGG - chr2.1:17248273-17248292 MS.gene35299:CDS 35.0%
! GTTGAAACTTGGCATGTTTT+TGG + chr2.1:17249166-17249185 None:intergenic 35.0%
!! TTGACGAGTCTTGTTGATTT+GGG + chr2.1:17248461-17248480 None:intergenic 35.0%
AACATTGTGGTGATCTTAGG+TGG - chr2.1:17249309-17249328 MS.gene35299:CDS 40.0%
ACAAGACTCGTCAACATCTA+TGG - chr2.1:17248467-17248486 MS.gene35299:CDS 40.0%
AGGACACATGAGAAACATTG+TGG - chr2.1:17249296-17249315 MS.gene35299:CDS 40.0%
ATGAGTCAGAACCATTGGAT+TGG - chr2.1:17248706-17248725 MS.gene35299:CDS 40.0%
ATTGAAAAGGAGGGAATTGG+AGG + chr2.1:17248379-17248398 None:intergenic 40.0%
ATTGCTCTTAGTTTCCCTCA+AGG - chr2.1:17248564-17248583 MS.gene35299:CDS 40.0%
ATTTCAAGGACAACACCTCT+AGG - chr2.1:17248252-17248271 MS.gene35299:CDS 40.0%
CTTTCACCAAACCAATCCAA+TGG + chr2.1:17248720-17248739 None:intergenic 40.0%
GAGATTGAAAAGGAGGGAAT+TGG + chr2.1:17248382-17248401 None:intergenic 40.0%
GAGTAGAGAGATTGAAAAGG+AGG + chr2.1:17248389-17248408 None:intergenic 40.0%
GATAAGCTTATGGGACATGT+TGG - chr2.1:17249365-17249384 MS.gene35299:CDS 40.0%
GATGTCATTGCCAAATTCCA+TGG - chr2.1:17248329-17248348 MS.gene35299:CDS 40.0%
GCAAGTGAACATCATTGATG+TGG + chr2.1:17248538-17248557 None:intergenic 40.0%
GGAAACTAAGAGCAATCTGT+TGG + chr2.1:17248560-17248579 None:intergenic 40.0%
GGGTCATCTTATAATTGGGT+CGG - chr2.1:17248894-17248913 MS.gene35299:CDS 40.0%
TCAAGAGGGATGCAAATTTG+AGG - chr2.1:17249006-17249025 MS.gene35299:CDS 40.0%
TCATTGCCAAATTCCATGGA+AGG - chr2.1:17248333-17248352 MS.gene35299:CDS 40.0%
TTGAAAAGGAGGGAATTGGA+GGG + chr2.1:17248378-17248397 None:intergenic 40.0%
TTTCAAGGACAACACCTCTA+GGG - chr2.1:17248253-17248272 MS.gene35299:CDS 40.0%
!! ACACCTCTAGGGTTTGAAAT+AGG - chr2.1:17248264-17248283 MS.gene35299:CDS 40.0%
!! GGACATGTTGGTTTGTTTGT+GGG - chr2.1:17249377-17249396 MS.gene35299:CDS 40.0%
!! GTTGACGAGTCTTGTTGATT+TGG + chr2.1:17248462-17248481 None:intergenic 40.0%
!! TCAGAACCATTGGATTGGTT+TGG - chr2.1:17248711-17248730 MS.gene35299:CDS 40.0%
!! TGACGAGTCTTGTTGATTTG+GGG + chr2.1:17248460-17248479 None:intergenic 40.0%
AGGATGCATATGAGAGCTCA+TGG - chr2.1:17249026-17249045 MS.gene35299:CDS 45.0%
GATGCATATGAGAGCTCATG+GGG - chr2.1:17249028-17249047 MS.gene35299:CDS 45.0%
GGATGCATATGAGAGCTCAT+GGG - chr2.1:17249027-17249046 MS.gene35299:CDS 45.0%
TTCCATCTACACCCTTCTTG+AGG + chr2.1:17249143-17249162 None:intergenic 45.0%
TTGTGGCAAAGGGTTCAAGA+GGG - chr2.1:17248992-17249011 MS.gene35299:CDS 45.0%
TTTGTGGCAAAGGGTTCAAG+AGG - chr2.1:17248991-17249010 MS.gene35299:CDS 45.0%
TTTGTTTGTGGGACATCACC+CGG - chr2.1:17249388-17249407 MS.gene35299:CDS 45.0%
! CATGTGGCACTACCTTTTCT+AGG - chr2.1:17249339-17249358 MS.gene35299:CDS 45.0%
!! GGGACATGTTGGTTTGTTTG+TGG - chr2.1:17249376-17249395 MS.gene35299:CDS 45.0%
CTTAGGTGGCAGTGTTCATG+TGG - chr2.1:17249323-17249342 MS.gene35299:CDS 50.0%
GGACATCACCCGGTTATCAA+TGG - chr2.1:17249398-17249417 MS.gene35299:CDS 50.0%
TAATTGGGTCGGCCCAGAAT+TGG - chr2.1:17248905-17248924 MS.gene35299:CDS 50.0%
! GTCCTCAAGAAGGGTGTAGA+TGG - chr2.1:17249138-17249157 MS.gene35299:CDS 50.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 17248249 17249448 17248249 ID=MS.gene35299
chr2.1 mRNA 17248249 17249448 17248249 ID=MS.gene35299.t1;Parent=MS.gene35299
chr2.1 exon 17248249 17249448 17248249 ID=MS.gene35299.t1.exon1;Parent=MS.gene35299.t1
chr2.1 CDS 17248249 17249448 17248249 ID=cds.MS.gene35299.t1;Parent=MS.gene35299.t1
Gene Sequence

>MS.gene35299

ATGATTTCAAGGACAACACCTCTAGGGTTTGAAATAGGAAATCAAGGCCTACAAATGTTTCCTATGATCACGGAAGATTTGATGTCATTGCCAAATTCCATGGAAGGAATTTCTAGTTCAACTTTACCCTCCAATTCCCTCCTTTTCAATCTCTCTACTCTCAAAGACAAGCTCAATCAAGTGCAAACTCTTGTTGGTGTCATTCTCTCCCCAAATCAACAAGACTCGTCAACATCTATGGCATTATCCACAATGAACTCCACAATACAAGAAATCATTGTAACTTCCACATCAATGATGTTCACTTGCCAACAGATTGCTCTTAGTTTCCCTCAAGGTACTACTTCTATTAAGTGTACAAACCAAGAATTTCAAAAGCAAAAATCTAATATTCAATCAAATTTTGGCAACAATGACACAAACATTGATCTTAGAGGTCAAAGCATATTTTCTAATAATGAGTCAGAACCATTGGATTGGTTTGGTGAAAGCTATAATAGTAACATTAATTATAGGCTCAAGGATGATATTGATGATCAAAGGGTTGATCATCAAATTAGCGAAACCAATAATAGTATAAGAGGGTCTAATGAAGATATTTCAACGAAGAATAATTCTGATCGTGACGATACATGCTTAAAATTTGGGTCATCTTATAATTGGGTCGGCCCAGAATTGGACAATGAGATAGTTGAATTAGACGCGGCCGATTTATTAGCCAAGTACACACATTATTGTCAAGTTTGTGGCAAAGGGTTCAAGAGGGATGCAAATTTGAGGATGCATATGAGAGCTCATGGGGATGAGTACAAGAGTAGTGCAGCATTGAGCAATCCAATTACTAAGAATAGAGATTATTTGATGAGTATGAAAAATAAGAAATATTCATGTCCTCAAGAAGGGTGTAGATGGAACCAAAAACATGCCAAGTTTCAACCTTTGAAGTCATTGATTTGTGCTAAGAATCATTACAAGAGAAGTCATTGTCCCAAAATGTATGTATGCAAGAGGTGTAATTTGAAGCAATTTTCTGTTCTTTCTGATTTGAGGACACATGAGAAACATTGTGGTGATCTTAGGTGGCAGTGTTCATGTGGCACTACCTTTTCTAGGAAAGATAAGCTTATGGGACATGTTGGTTTGTTTGTGGGACATCACCCGGTTATCAATGGTTTGTCATATAGTACTCACACAAGTTAG
Protein sequence

>MS.gene35299.t1

MISRTTPLGFEIGNQGLQMFPMITEDLMSLPNSMEGISSSTLPSNSLLFNLSTLKDKLNQVQTLVGVILSPNQQDSSTSMALSTMNSTIQEIIVTSTSMMFTCQQIALSFPQGTTSIKCTNQEFQKQKSNIQSNFGNNDTNIDLRGQSIFSNNESEPLDWFGESYNSNINYRLKDDIDDQRVDHQISETNNSIRGSNEDISTKNNSDRDDTCLKFGSSYNWVGPELDNEIVELDAADLLAKYTHYCQVCGKGFKRDANLRMHMRAHGDEYKSSAALSNPITKNRDYLMSMKNKKYSCPQEGCRWNQKHAKFQPLKSLICAKNHYKRSHCPKMYVCKRCNLKQFSVLSDLRTHEKHCGDLRWQCSCGTTFSRKDKLMGHVGLFVGHHPVINGLSYSTHTS