Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35617.t1 | XP_013460852.1 | 93.2 | 146 | 10 | 0 | 1 | 146 | 17 | 162 | 2.10E-72 | 281.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35617.t1 | P33050 | 38.0 | 150 | 89 | 1 | 1 | 146 | 20 | 169 | 2.3e-22 | 106.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35617.t1 | B7FN25 | 93.2 | 146 | 10 | 0 | 1 | 146 | 17 | 162 | 1.5e-72 | 281.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053143 | MS.gene35617 | 0.801748 | 7.78E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35617.t1 | MTR_3g073990 | 93.151 | 146 | 10 | 0 | 1 | 146 | 17 | 162 | 1.29e-101 | 288 |
| MS.gene35617.t1 | MTR_3g087540 | 41.406 | 128 | 73 | 2 | 12 | 137 | 25 | 152 | 3.04e-31 | 110 |
| MS.gene35617.t1 | MTR_2g016020 | 38.168 | 131 | 77 | 2 | 12 | 139 | 28 | 157 | 1.37e-23 | 90.5 |
| MS.gene35617.t1 | MTR_2g077940 | 33.594 | 128 | 82 | 2 | 12 | 139 | 43 | 167 | 7.37e-19 | 78.6 |
| MS.gene35617.t1 | MTR_2g077960 | 28.966 | 145 | 93 | 3 | 2 | 139 | 20 | 161 | 1.83e-15 | 69.7 |
| MS.gene35617.t1 | MTR_2g077080 | 28.906 | 128 | 88 | 3 | 12 | 139 | 36 | 160 | 6.22e-12 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35617.t1 | AT4G08685 | 48.611 | 144 | 74 | 0 | 1 | 144 | 16 | 159 | 3.74e-49 | 155 |
| MS.gene35617.t1 | AT5G10130 | 40.441 | 136 | 81 | 0 | 3 | 138 | 19 | 154 | 4.06e-28 | 102 |
| MS.gene35617.t1 | AT1G78040 | 35.849 | 159 | 89 | 3 | 1 | 146 | 8 | 166 | 4.82e-27 | 99.8 |
| MS.gene35617.t1 | AT1G78040 | 35.849 | 159 | 89 | 3 | 1 | 146 | 8 | 166 | 4.82e-27 | 99.8 |
| MS.gene35617.t1 | AT1G78040 | 35.849 | 159 | 89 | 3 | 1 | 146 | 8 | 166 | 4.82e-27 | 99.8 |
| MS.gene35617.t1 | AT4G18596 | 34.091 | 132 | 83 | 2 | 12 | 139 | 33 | 164 | 1.06e-20 | 83.2 |
| MS.gene35617.t1 | AT5G45880 | 33.333 | 132 | 84 | 2 | 12 | 139 | 35 | 166 | 1.19e-20 | 83.2 |
| MS.gene35617.t1 | AT1G29140 | 35.075 | 134 | 83 | 2 | 10 | 139 | 30 | 163 | 1.81e-20 | 82.8 |
| MS.gene35617.t1 | AT4G18596 | 34.091 | 132 | 83 | 2 | 12 | 139 | 79 | 210 | 2.47e-20 | 83.6 |
Find 42 sgRNAs with CRISPR-Local
Find 75 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCAAGCAGAAAGGGTTCT+TGG | 0.299969 | 3.3:-63848047 | None:intergenic |
| CAAGAACCCTTTCTGCTTGA+AGG | 0.359097 | 3.3:+63848048 | MS.gene35617:CDS |
| ATTGGCAAACCTGTCATTAG+AGG | 0.373550 | 3.3:-63849128 | None:intergenic |
| CCAGTTGCATCAGTCTTCCC+TGG | 0.379925 | 3.3:-63848962 | None:intergenic |
| AAGCAGAAAGGGTTCTTGGC+TGG | 0.404947 | 3.3:-63848043 | None:intergenic |
| CCTCAAGCAAATGCAAGAAT+TGG | 0.407401 | 3.3:+63849198 | MS.gene35617:CDS |
| ATGTCAGAGGAGGTTGCTTC+TGG | 0.416314 | 3.3:+63849163 | MS.gene35617:CDS |
| AGAGGCAATGCCGTTGTAAC+GGG | 0.421384 | 3.3:-63849110 | None:intergenic |
| TGTTCTTCCAGCGATGGTTA+TGG | 0.427430 | 3.3:+63848012 | None:intergenic |
| TCTGCTATCACTCACATTGC+TGG | 0.439139 | 3.3:+63848112 | MS.gene35617:CDS |
| GTATGTGACCCTTGCCGTGC+TGG | 0.440078 | 3.3:+63848079 | MS.gene35617:CDS |
| CTTAGGTGCCGAGGTTATGC+TGG | 0.455452 | 3.3:+63848898 | MS.gene35617:intron |
| AGCAGAAAGGGTTCTTGGCT+GGG | 0.458995 | 3.3:-63848042 | None:intergenic |
| CCAGGGAAGACTGATGCAAC+TGG | 0.462248 | 3.3:+63848962 | MS.gene35617:CDS |
| CTTTCACTGCAACTACGCTC+AGG | 0.464860 | 3.3:-63849052 | None:intergenic |
| ATATACAATCTATGTGGATC+AGG | 0.471477 | 3.3:+63848988 | MS.gene35617:CDS |
| CACGACCCTTCAAGCAGAAA+GGG | 0.481464 | 3.3:-63848054 | None:intergenic |
| CCAATTCTTGCATTTGCTTG+AGG | 0.488555 | 3.3:-63849198 | None:intergenic |
| AGGGTTCTTGGCTGGGCGAA+TGG | 0.488766 | 3.3:-63848035 | None:intergenic |
| AGTTGCAGTGAAAGAGCACC+TGG | 0.493527 | 3.3:+63849061 | MS.gene35617:CDS |
| GAAGTTGTGTTCTCAAAGCC+AGG | 0.495130 | 3.3:+63848944 | MS.gene35617:CDS |
| AAGAACCCTTTCTGCTTGAA+GGG | 0.515228 | 3.3:+63848049 | MS.gene35617:CDS |
| TAGAGGCAATGCCGTTGTAA+CGG | 0.528529 | 3.3:-63849111 | None:intergenic |
| ACACGACCCTTCAAGCAGAA+AGG | 0.545423 | 3.3:-63848055 | None:intergenic |
| ACGGCATTGCCTCTAATGAC+AGG | 0.562012 | 3.3:+63849119 | MS.gene35617:CDS |
| CTTGCATTCCAGCATAACCT+CGG | 0.573523 | 3.3:-63848906 | None:intergenic |
| GTTATTCTCACCCGTTACAA+CGG | 0.576439 | 3.3:+63849100 | MS.gene35617:CDS |
| ACACGTGCTTGGTCACGCCC+AGG | 0.584341 | 3.3:-63849079 | None:intergenic |
| TGACATGTATAACATTGCAT+TGG | 0.586829 | 3.3:-63849146 | None:intergenic |
| GTTTCATAACCAGCACGGCA+AGG | 0.587642 | 3.3:-63848088 | None:intergenic |
| GGTTGCTTCTGGCTGTGCTG+AGG | 0.590498 | 3.3:+63849174 | MS.gene35617:CDS |
| TTTCACTGCAACTACGCTCA+GGG | 0.598609 | 3.3:-63849051 | None:intergenic |
| TGGAGAATATACAATCTATG+TGG | 0.605358 | 3.3:+63848982 | MS.gene35617:CDS |
| ATGAATCTGCTTAGGTGCCG+AGG | 0.607207 | 3.3:+63848889 | MS.gene35617:intron |
| TTTCATAACCAGCACGGCAA+GGG | 0.614551 | 3.3:-63848087 | None:intergenic |
| GGGTGAGAATAACACGTGCT+TGG | 0.627588 | 3.3:-63849090 | None:intergenic |
| TGCAATGTTATACATGTCAG+AGG | 0.631370 | 3.3:+63849150 | MS.gene35617:CDS |
| AAGTTGTGTTCTCAAAGCCA+GGG | 0.636901 | 3.3:+63848945 | MS.gene35617:CDS |
| TTATGCTGGAATGCAAGCAA+AGG | 0.653268 | 3.3:+63848912 | MS.gene35617:CDS |
| CAGAAGTTTCATAACCAGCA+CGG | 0.663268 | 3.3:-63848093 | None:intergenic |
| AATGTTATACATGTCAGAGG+AGG | 0.692196 | 3.3:+63849153 | MS.gene35617:CDS |
| GTTGCAGTGAAAGAGCACCT+GGG | 0.719508 | 3.3:+63849062 | MS.gene35617:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATATCTTTGTTAATTAA+TGG | + | chr3.3:63848441-63848460 | MS.gene35617:intron | 10.0% |
| !!! | ACTTTAAATTCTTTAAATTT+TGG | + | chr3.3:63848232-63848251 | MS.gene35617:intron | 10.0% |
| !!! | TTAATTAGAAGTATAAAAAA+GGG | + | chr3.3:63848790-63848809 | MS.gene35617:intron | 10.0% |
| !!! | TTTAATTAGAAGTATAAAAA+AGG | + | chr3.3:63848789-63848808 | MS.gene35617:intron | 10.0% |
| !! | ATTATATGATAGAACATTAT+GGG | + | chr3.3:63848683-63848702 | MS.gene35617:intron | 15.0% |
| !! | TATTATATGATAGAACATTA+TGG | + | chr3.3:63848682-63848701 | MS.gene35617:intron | 15.0% |
| !!! | AATTCTTTAAATTTTGGAAA+TGG | + | chr3.3:63848238-63848257 | MS.gene35617:intron | 15.0% |
| !! | AATAGTGAAATTAAACCAAA+AGG | - | chr3.3:63848208-63848227 | None:intergenic | 20.0% |
| !! | ATAGTGAAATTAAACCAAAA+GGG | - | chr3.3:63848207-63848226 | None:intergenic | 20.0% |
| !! | ATCTATTGTTTCAAATAATC+TGG | - | chr3.3:63848523-63848542 | None:intergenic | 20.0% |
| !! | CACAATATTAGTGTAATATA+GGG | + | chr3.3:63848370-63848389 | MS.gene35617:intron | 20.0% |
| !! | CTTATAATTTATAATCCTCA+TGG | + | chr3.3:63848817-63848836 | MS.gene35617:intron | 20.0% |
| !! | TCACAATATTAGTGTAATAT+AGG | + | chr3.3:63848369-63848388 | MS.gene35617:intron | 20.0% |
| !!! | CAATTTTGCTTTAGATTTTA+GGG | + | chr3.3:63848275-63848294 | MS.gene35617:intron | 20.0% |
| !!! | TCAATTTTGCTTTAGATTTT+AGG | + | chr3.3:63848274-63848293 | MS.gene35617:intron | 20.0% |
| ! | TCGAATGAAAAACTTAAACT+TGG | - | chr3.3:63848175-63848194 | None:intergenic | 25.0% |
| ! | TTTGATTAATGAATCTGCTT+AGG | + | chr3.3:63848881-63848900 | MS.gene35617:intron | 25.0% |
| AAAGAGATCTGAGAGTAAAA+AGG | - | chr3.3:63848143-63848162 | None:intergenic | 30.0% | |
| AAGAGATCTGAGAGTAAAAA+GGG | - | chr3.3:63848142-63848161 | None:intergenic | 30.0% | |
| ATATACAATCTATGTGGATC+AGG | + | chr3.3:63848988-63849007 | MS.gene35617:CDS | 30.0% | |
| CTCAATTATCTCAAAAAGAG+TGG | - | chr3.3:63848330-63848349 | None:intergenic | 30.0% | |
| TGACATGTATAACATTGCAT+TGG | - | chr3.3:63849149-63849168 | None:intergenic | 30.0% | |
| TGGAGAATATACAATCTATG+TGG | + | chr3.3:63848982-63849001 | MS.gene35617:CDS | 30.0% | |
| TGTGTTAACAAACTACCATA+TGG | - | chr3.3:63848853-63848872 | None:intergenic | 30.0% | |
| TGTTTGATTCATGTAACCAT+TGG | + | chr3.3:63848708-63848727 | MS.gene35617:intron | 30.0% | |
| TTGTTAAATGAATGACCTTG+CGG | + | chr3.3:63848482-63848501 | MS.gene35617:intron | 30.0% | |
| !!! | GCTTTAGATTTTAGGGTTTA+GGG | + | chr3.3:63848282-63848301 | MS.gene35617:intron | 30.0% |
| !!! | TGCTTTAGATTTTAGGGTTT+AGG | + | chr3.3:63848281-63848300 | MS.gene35617:intron | 30.0% |
| AATGTTATACATGTCAGAGG+AGG | + | chr3.3:63849153-63849172 | MS.gene35617:CDS | 35.0% | |
| ACAACAGTAGTAACAACCAA+TGG | - | chr3.3:63848727-63848746 | None:intergenic | 35.0% | |
| AGAGATCTGAGAGTAAAAAG+GGG | - | chr3.3:63848141-63848160 | None:intergenic | 35.0% | |
| ATTGAGTATCAGATCTGATC+TGG | + | chr3.3:63848344-63848363 | MS.gene35617:intron | 35.0% | |
| TATGGAAAATGTTCTCCATG+AGG | - | chr3.3:63848835-63848854 | None:intergenic | 35.0% | |
| TGCAATGTTATACATGTCAG+AGG | + | chr3.3:63849150-63849169 | MS.gene35617:CDS | 35.0% | |
| TTGCTTCTAATGCAAAGATC+TGG | + | chr3.3:63848635-63848654 | MS.gene35617:intron | 35.0% | |
| ! | CATGGAGAACATTTTCCATA+TGG | + | chr3.3:63848835-63848854 | MS.gene35617:intron | 35.0% |
| !! | TTCGAATTTGTTCACCCTTT+TGG | + | chr3.3:63848190-63848209 | MS.gene35617:intron | 35.0% |
| AAGAACCCTTTCTGCTTGAA+GGG | + | chr3.3:63848049-63848068 | MS.gene35617:CDS | 40.0% | |
| AAGTTGTGTTCTCAAAGCCA+GGG | + | chr3.3:63848945-63848964 | MS.gene35617:CDS | 40.0% | |
| ATTGGCAAACCTGTCATTAG+AGG | - | chr3.3:63849131-63849150 | None:intergenic | 40.0% | |
| CAGAAGTTTCATAACCAGCA+CGG | - | chr3.3:63848096-63848115 | None:intergenic | 40.0% | |
| CCAATTCTTGCATTTGCTTG+AGG | - | chr3.3:63849201-63849220 | None:intergenic | 40.0% | |
| CCTCAAGCAAATGCAAGAAT+TGG | + | chr3.3:63849198-63849217 | MS.gene35617:CDS | 40.0% | |
| GTTATTCTCACCCGTTACAA+CGG | + | chr3.3:63849100-63849119 | MS.gene35617:CDS | 40.0% | |
| TTACAAGATTTCACTCCGCA+AGG | - | chr3.3:63848500-63848519 | None:intergenic | 40.0% | |
| TTATGCTGGAATGCAAGCAA+AGG | + | chr3.3:63848912-63848931 | MS.gene35617:CDS | 40.0% | |
| !!! | AGATTTTAGGGTTTAGGGTG+TGG | + | chr3.3:63848287-63848306 | MS.gene35617:intron | 40.0% |
| CAAGAACCCTTTCTGCTTGA+AGG | + | chr3.3:63848048-63848067 | MS.gene35617:CDS | 45.0% | |
| CTTCAAGCAGAAAGGGTTCT+TGG | - | chr3.3:63848050-63848069 | None:intergenic | 45.0% | |
| CTTGCATTCCAGCATAACCT+CGG | - | chr3.3:63848909-63848928 | None:intergenic | 45.0% | |
| GAAGTTGTGTTCTCAAAGCC+AGG | + | chr3.3:63848944-63848963 | MS.gene35617:CDS | 45.0% | |
| TAGAGGCAATGCCGTTGTAA+CGG | - | chr3.3:63849114-63849133 | None:intergenic | 45.0% | |
| TCTGCTATCACTCACATTGC+TGG | + | chr3.3:63848112-63848131 | MS.gene35617:CDS | 45.0% | |
| TTTCACTGCAACTACGCTCA+GGG | - | chr3.3:63849054-63849073 | None:intergenic | 45.0% | |
| TTTCATAACCAGCACGGCAA+GGG | - | chr3.3:63848090-63848109 | None:intergenic | 45.0% | |
| AAGCAGAAAGGGTTCTTGGC+TGG | - | chr3.3:63848046-63848065 | None:intergenic | 50.0% | |
| ACACGACCCTTCAAGCAGAA+AGG | - | chr3.3:63848058-63848077 | None:intergenic | 50.0% | |
| ACGGCATTGCCTCTAATGAC+AGG | + | chr3.3:63849119-63849138 | MS.gene35617:CDS | 50.0% | |
| AGAGGCAATGCCGTTGTAAC+GGG | - | chr3.3:63849113-63849132 | None:intergenic | 50.0% | |
| AGCAGAAAGGGTTCTTGGCT+GGG | - | chr3.3:63848045-63848064 | None:intergenic | 50.0% | |
| ATGTCAGAGGAGGTTGCTTC+TGG | + | chr3.3:63849163-63849182 | MS.gene35617:CDS | 50.0% | |
| CACGACCCTTCAAGCAGAAA+GGG | - | chr3.3:63848057-63848076 | None:intergenic | 50.0% | |
| CTTTCACTGCAACTACGCTC+AGG | - | chr3.3:63849055-63849074 | None:intergenic | 50.0% | |
| GGGTGAGAATAACACGTGCT+TGG | - | chr3.3:63849093-63849112 | None:intergenic | 50.0% | |
| GTTTCATAACCAGCACGGCA+AGG | - | chr3.3:63848091-63848110 | None:intergenic | 50.0% | |
| ! | ATGAATCTGCTTAGGTGCCG+AGG | + | chr3.3:63848889-63848908 | MS.gene35617:intron | 50.0% |
| !! | AGTTGCAGTGAAAGAGCACC+TGG | + | chr3.3:63849061-63849080 | MS.gene35617:CDS | 50.0% |
| !! | GTTGCAGTGAAAGAGCACCT+GGG | + | chr3.3:63849062-63849081 | MS.gene35617:CDS | 50.0% |
| CCAGGGAAGACTGATGCAAC+TGG | + | chr3.3:63848962-63848981 | MS.gene35617:CDS | 55.0% | |
| CCAGTTGCATCAGTCTTCCC+TGG | - | chr3.3:63848965-63848984 | None:intergenic | 55.0% | |
| !! | CTTAGGTGCCGAGGTTATGC+TGG | + | chr3.3:63848898-63848917 | MS.gene35617:intron | 55.0% |
| AGGGTTCTTGGCTGGGCGAA+TGG | - | chr3.3:63848038-63848057 | None:intergenic | 60.0% | |
| GTATGTGACCCTTGCCGTGC+TGG | + | chr3.3:63848079-63848098 | MS.gene35617:CDS | 60.0% | |
| ! | GGTTGCTTCTGGCTGTGCTG+AGG | + | chr3.3:63849174-63849193 | MS.gene35617:CDS | 60.0% |
| ACACGTGCTTGGTCACGCCC+AGG | - | chr3.3:63849082-63849101 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 63848025 | 63849234 | 63848025 | ID=MS.gene35617 |
| chr3.3 | mRNA | 63848025 | 63849234 | 63848025 | ID=MS.gene35617.t1;Parent=MS.gene35617 |
| chr3.3 | exon | 63848025 | 63848133 | 63848025 | ID=MS.gene35617.t1.exon1;Parent=MS.gene35617.t1 |
| chr3.3 | CDS | 63848025 | 63848133 | 63848025 | ID=cds.MS.gene35617.t1;Parent=MS.gene35617.t1 |
| chr3.3 | exon | 63848903 | 63849234 | 63848903 | ID=MS.gene35617.t1.exon2;Parent=MS.gene35617.t1 |
| chr3.3 | CDS | 63848903 | 63849234 | 63848903 | ID=cds.MS.gene35617.t1;Parent=MS.gene35617.t1 |
| Gene Sequence |
| Protein sequence |
