Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35766.t1 | RHN75615.1 | 76.6 | 124 | 29 | 0 | 1 | 124 | 1 | 124 | 1.60E-28 | 135.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35766.t1 | A0A396JGX3 | 76.6 | 124 | 29 | 0 | 1 | 124 | 1 | 124 | 1.2e-28 | 135.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052463 | MS.gene35766 | 0.800706 | 1.27E-48 | -1.69E-46 |
| MS.gene055513 | MS.gene35766 | 0.805195 | 1.50E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35766.t1 | MTR_2g089190 | 95.868 | 121 | 5 | 0 | 4 | 124 | 1 | 121 | 1.16e-65 | 195 |
| MS.gene35766.t1 | MTR_0945s0020 | 48.718 | 117 | 53 | 1 | 4 | 120 | 1 | 110 | 7.79e-19 | 76.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGATAACTCATATCTTCT+TGG | 0.242180 | 2.1:-12827952 | MS.gene35766:CDS |
| TTTCCCATCCTTCTATAATC+TGG | 0.304837 | 2.1:+12827765 | None:intergenic |
| CATTCGTGTTACAAGGAAAA+AGG | 0.319879 | 2.1:-12827655 | MS.gene35766:CDS |
| CACAATGAGCAAAGTTGGCT+TGG | 0.422205 | 2.1:-12827982 | MS.gene35766:CDS |
| CCATTCTTCATCATCAGAAA+TGG | 0.435323 | 2.1:-12827739 | MS.gene35766:CDS |
| CTATGGCTCCAGATTATAGA+AGG | 0.440541 | 2.1:-12827773 | MS.gene35766:CDS |
| GGTGGAGGTGTTATCATTGG+TGG | 0.460496 | 2.1:-12827714 | MS.gene35766:CDS |
| ATGTTCACAATGAGCAAAGT+TGG | 0.469197 | 2.1:-12827987 | MS.gene35766:CDS |
| TTTGCTACATTCGTGTTACA+AGG | 0.470423 | 2.1:-12827662 | MS.gene35766:CDS |
| GTGGAGGTGTTATCATTGGT+GGG | 0.484241 | 2.1:-12827713 | MS.gene35766:CDS |
| GCTGGTGGAGGTGTTATCAT+TGG | 0.499326 | 2.1:-12827717 | MS.gene35766:CDS |
| GGCTCCAGATTATAGAAGGA+TGG | 0.502778 | 2.1:-12827769 | MS.gene35766:CDS |
| GATAGTGATGGTGGTGGTGT+TGG | 0.521138 | 2.1:-12827633 | MS.gene35766:CDS |
| TCTAGCTCATCAAAGGAAGA+AGG | 0.538176 | 2.1:-12827822 | MS.gene35766:CDS |
| CTCTGAGGCTCCAACTCCAA+TGG | 0.550694 | 2.1:-12827898 | MS.gene35766:CDS |
| TAAAATTTCTAGCTCATCAA+AGG | 0.552169 | 2.1:-12827829 | MS.gene35766:CDS |
| CTTGGTAGCACTTCAAGCCA+TGG | 0.557559 | 2.1:-12827934 | MS.gene35766:CDS |
| GCTCCAGATTATAGAAGGAT+GGG | 0.558142 | 2.1:-12827768 | MS.gene35766:CDS |
| TCATCAGAAATGGCTGGTGG+AGG | 0.566796 | 2.1:-12827729 | MS.gene35766:CDS |
| TCTTCATCATCAGAAATGGC+TGG | 0.582426 | 2.1:-12827735 | MS.gene35766:CDS |
| AGAGTAATGAACTTCTTCCA+TGG | 0.586057 | 2.1:+12827917 | None:intergenic |
| ATCAAAAGAAGAAAGCGCTA+TGG | 0.617046 | 2.1:-12827790 | MS.gene35766:CDS |
| CCATTTCTGATGATGAAGAA+TGG | 0.627104 | 2.1:+12827739 | None:intergenic |
| TCATCATCAGAAATGGCTGG+TGG | 0.633404 | 2.1:-12827732 | MS.gene35766:CDS |
| GGAAGAAGTTCATTACTCTG+AGG | 0.699853 | 2.1:-12827913 | MS.gene35766:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAAAATTTCTAGCTCATCAA+AGG | - | chr2.1:12827784-12827803 | MS.gene35766:CDS | 25.0% |
| ! | CTTGATAACTCATATCTTCT+TGG | - | chr2.1:12827661-12827680 | MS.gene35766:CDS | 30.0% |
| !! | CTATGATTTTTAAGCTCCAT+TGG | + | chr2.1:12827734-12827753 | None:intergenic | 30.0% |
| ACAAGGAAAAAGGATAGTGA+TGG | - | chr2.1:12827968-12827987 | MS.gene35766:CDS | 35.0% | |
| AGAGTAATGAACTTCTTCCA+TGG | + | chr2.1:12827699-12827718 | None:intergenic | 35.0% | |
| ATGTTCACAATGAGCAAAGT+TGG | - | chr2.1:12827626-12827645 | MS.gene35766:CDS | 35.0% | |
| CATTCGTGTTACAAGGAAAA+AGG | - | chr2.1:12827958-12827977 | MS.gene35766:CDS | 35.0% | |
| CCATTCTTCATCATCAGAAA+TGG | - | chr2.1:12827874-12827893 | MS.gene35766:CDS | 35.0% | |
| TTTCCCATCCTTCTATAATC+TGG | + | chr2.1:12827851-12827870 | None:intergenic | 35.0% | |
| TTTGCTACATTCGTGTTACA+AGG | - | chr2.1:12827951-12827970 | MS.gene35766:CDS | 35.0% | |
| ! | CCATTTCTGATGATGAAGAA+TGG | + | chr2.1:12827877-12827896 | None:intergenic | 35.0% |
| !! | ATCAAAAGAAGAAAGCGCTA+TGG | - | chr2.1:12827823-12827842 | MS.gene35766:CDS | 35.0% |
| !! | TTTTTAAGCTCCATTGGAGT+TGG | + | chr2.1:12827728-12827747 | None:intergenic | 35.0% |
| AGGAAAAAGGATAGTGATGG+TGG | - | chr2.1:12827971-12827990 | MS.gene35766:CDS | 40.0% | |
| CTATGGCTCCAGATTATAGA+AGG | - | chr2.1:12827840-12827859 | MS.gene35766:CDS | 40.0% | |
| GCTCCAGATTATAGAAGGAT+GGG | - | chr2.1:12827845-12827864 | MS.gene35766:CDS | 40.0% | |
| GGAAGAAGTTCATTACTCTG+AGG | - | chr2.1:12827700-12827719 | MS.gene35766:CDS | 40.0% | |
| TCTTCATCATCAGAAATGGC+TGG | - | chr2.1:12827878-12827897 | MS.gene35766:CDS | 40.0% | |
| ! | TCTAGCTCATCAAAGGAAGA+AGG | - | chr2.1:12827791-12827810 | MS.gene35766:CDS | 40.0% |
| !! | AAAAAGGATAGTGATGGTGG+TGG | - | chr2.1:12827974-12827993 | MS.gene35766:CDS | 40.0% |
| CACAATGAGCAAAGTTGGCT+TGG | - | chr2.1:12827631-12827650 | MS.gene35766:CDS | 45.0% | |
| GGCTCCAGATTATAGAAGGA+TGG | - | chr2.1:12827844-12827863 | MS.gene35766:CDS | 45.0% | |
| TCATCATCAGAAATGGCTGG+TGG | - | chr2.1:12827881-12827900 | MS.gene35766:CDS | 45.0% | |
| !! | GTGGAGGTGTTATCATTGGT+GGG | - | chr2.1:12827900-12827919 | MS.gene35766:CDS | 45.0% |
| TCATCAGAAATGGCTGGTGG+AGG | - | chr2.1:12827884-12827903 | MS.gene35766:CDS | 50.0% | |
| ! | CTTGGTAGCACTTCAAGCCA+TGG | - | chr2.1:12827679-12827698 | MS.gene35766:CDS | 50.0% |
| !! | GATAGTGATGGTGGTGGTGT+TGG | - | chr2.1:12827980-12827999 | MS.gene35766:CDS | 50.0% |
| !! | GCTGGTGGAGGTGTTATCAT+TGG | - | chr2.1:12827896-12827915 | MS.gene35766:CDS | 50.0% |
| !! | GGTGGAGGTGTTATCATTGG+TGG | - | chr2.1:12827899-12827918 | MS.gene35766:CDS | 50.0% |
| !! | CTCTGAGGCTCCAACTCCAA+TGG | - | chr2.1:12827715-12827734 | MS.gene35766:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 12827626 | 12828009 | 12827626 | ID=MS.gene35766 |
| chr2.1 | mRNA | 12827626 | 12828009 | 12827626 | ID=MS.gene35766.t1;Parent=MS.gene35766 |
| chr2.1 | exon | 12827626 | 12828009 | 12827626 | ID=MS.gene35766.t1.exon1;Parent=MS.gene35766.t1 |
| chr2.1 | CDS | 12827626 | 12828009 | 12827626 | ID=cds.MS.gene35766.t1;Parent=MS.gene35766.t1 |
| Gene Sequence |
| Protein sequence |
