AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35862

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 67 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTCTATATGGTTGTATCTA+TGG 0.163920 2.1:-13686805 None:intergenic
CATGAGATTGTTTCACAATT+AGG 0.168545 2.1:-13683868 None:intergenic
TACATTAAGCCCATAGATTT+TGG 0.191666 2.1:-13684941 None:intergenic
TTTCACCTTAAAAGGCTTAT+TGG 0.192883 2.1:-13686021 None:intergenic
AATGACTTAAAACACGTTTA+AGG 0.216795 2.1:-13684209 None:intergenic
TTATTGGATCTTGGAATTAT+TGG 0.221864 2.1:-13686005 None:intergenic
GATACTTTCCTGAATAAATT+AGG 0.237511 2.1:+13684727 MS.gene35862:CDS
TGGGGTGCTGTTCAAGGTTT+TGG 0.253190 2.1:+13686155 MS.gene35862:CDS
TGCTAACACCTAATTTATTC+AGG 0.253669 2.1:-13684735 None:intergenic
ATTCGAGCTGCCAAAATCTA+TGG 0.255519 2.1:+13684931 MS.gene35862:CDS
GCGACATTTCTTCGAGTTCT+TGG 0.264046 2.1:+13686774 MS.gene35862:CDS
ATGAGATTGTTTCACAATTA+GGG 0.284913 2.1:-13683867 None:intergenic
TTAAAAGGCTTATTGGATCT+TGG 0.305150 2.1:-13686014 None:intergenic
GAATATAGCTATTGTCTATA+TGG 0.327588 2.1:-13686817 None:intergenic
ATGATTTACTTCTTCGTCAT+AGG 0.356110 2.1:+13684112 MS.gene35862:CDS
TTCTAAAGCGGTTTGTGCTC+GGG 0.359775 2.1:-13686581 None:intergenic
CGTCATAGGCTTCACATTGT+TGG 0.374885 2.1:+13684126 MS.gene35862:CDS
GCAATATCAGATATGGATAA+TGG 0.377891 2.1:-13683671 None:intergenic
CAAAGTACGAATATCATATC+AGG 0.382928 2.1:+13683715 MS.gene35862:CDS
AGGTCCTGGGGTGCTGTTCA+AGG 0.393416 2.1:+13686149 MS.gene35862:CDS
CTTAGTGGCCGATTTCTTTG+TGG 0.398476 2.1:-13686309 None:intergenic
AGCTGCCGCAATATCAGATA+TGG 0.398548 2.1:-13683678 None:intergenic
TCAAATGGTCTGTACGATAC+TGG 0.406928 2.1:+13684895 MS.gene35862:CDS
TTAGTGGCCGATTTCTTTGT+GGG 0.416731 2.1:-13686308 None:intergenic
GTCGTTGACGACTCCAACAC+TGG 0.427647 2.1:+13686333 MS.gene35862:CDS
CTGATATGATATTCGTACTT+TGG 0.430254 2.1:-13683714 None:intergenic
CTCTCCCTCCTAGCTATGCC+TGG 0.432761 2.1:+13684171 MS.gene35862:CDS
GAGTCGTCAACGACTCTTAG+TGG 0.444824 2.1:-13686324 None:intergenic
ATTCCAATTGAAAATACATC+TGG 0.458943 2.1:+13684069 MS.gene35862:CDS
ACAGGCTGTTGAGTTGTGGC+TGG 0.462106 2.1:+13684032 MS.gene35862:intron
GTCTATATGGTTGTATCTAT+GGG 0.462194 2.1:-13686804 None:intergenic
GTCGTTATCTTGTGCTTGCA+AGG 0.469043 2.1:+13685979 MS.gene35862:CDS
AAAGTCGCCCACAAAGAAAT+CGG 0.470071 2.1:+13686301 MS.gene35862:CDS
GAAGAAGTAAATCATAGTTC+CGG 0.473021 2.1:-13684104 None:intergenic
TGATACAGCAGGTGCTGCTC+AGG 0.476534 2.1:+13684771 MS.gene35862:CDS
GACTAGATTGTAGCTTCAAA+TGG 0.479717 2.1:+13684880 MS.gene35862:intron
GTTCTAAAGCGGTTTGTGCT+CGG 0.485918 2.1:-13686582 None:intergenic
CCTCAAAATCAATATAGAAA+AGG 0.497773 2.1:-13686543 None:intergenic
CATTGTTGGCGAGGTGCAGT+TGG 0.503801 2.1:+13684140 MS.gene35862:CDS
CAGGCATAGCTAGGAGGGAG+AGG 0.512484 2.1:-13684170 None:intergenic
ATTGTGTTTACACTGAATGA+AGG 0.513002 2.1:+13686129 MS.gene35862:CDS
GCATCCTCGCTGTATGATCC+TGG 0.526215 2.1:-13683829 None:intergenic
TCTAAAGCGGTTTGTGCTCG+GGG 0.534280 2.1:-13686580 None:intergenic
TGTACTTGCAGAAGGAATCC+AGG 0.535619 2.1:+13684960 MS.gene35862:CDS
GAAAATGTTGATGATACAGC+AGG 0.537015 2.1:+13684760 MS.gene35862:CDS
CATCTGGTGGAAGCATTCAC+CGG 0.538869 2.1:+13684085 MS.gene35862:CDS
TTCTGACACCAGGCATAGCT+AGG 0.539323 2.1:-13684179 None:intergenic
TTTGGCGGTATTTGCAATGA+GGG 0.541837 2.1:+13686173 MS.gene35862:CDS
CCAATTGAAAATACATCTGG+TGG 0.545574 2.1:+13684072 MS.gene35862:CDS
AAAACCTTGAACAGCACCCC+AGG 0.551093 2.1:-13686153 None:intergenic
TTTACACTGAATGAAGGTCC+TGG 0.555043 2.1:+13686135 MS.gene35862:CDS
TTCGAGCTGCCAAAATCTAT+GGG 0.560483 2.1:+13684932 MS.gene35862:CDS
AACCGCTTTAGAACATCTGC+AGG 0.560617 2.1:+13686591 MS.gene35862:CDS
TCGTTATCTTGTGCTTGCAA+GGG 0.561749 2.1:+13685980 MS.gene35862:CDS
TCACTTGGCAGTTCCAGTGT+TGG 0.564118 2.1:-13686346 None:intergenic
GACACCAGGCATAGCTAGGA+GGG 0.564157 2.1:-13684175 None:intergenic
GGGCTTAATGTACTTGCAGA+AGG 0.566639 2.1:+13684952 MS.gene35862:CDS
TTACTGATTTAGGGGATACC+AGG 0.572631 2.1:+13683811 MS.gene35862:intron
TTACACTGAATGAAGGTCCT+GGG 0.575636 2.1:+13686136 MS.gene35862:CDS
TAGGCTTCACATTGTTGGCG+AGG 0.601015 2.1:+13684131 MS.gene35862:CDS
ATTATCCATATCTGATATTG+CGG 0.603030 2.1:+13683673 MS.gene35862:CDS
TGTGACAGGCTGTTGAGTTG+TGG 0.603950 2.1:+13684028 MS.gene35862:intron
TGACACCAGGCATAGCTAGG+AGG 0.623817 2.1:-13684176 None:intergenic
CACCTGCAGATGTTCTAAAG+CGG 0.635791 2.1:-13686593 None:intergenic
GAGGGACATAAATTTAACCA+AGG 0.667146 2.1:+13686191 MS.gene35862:CDS
TACACTGAATGAAGGTCCTG+GGG 0.677041 2.1:+13686137 MS.gene35862:CDS
GATACCAGGATCATACAGCG+AGG 0.785382 2.1:+13683825 MS.gene35862:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACAAGTATTTATATTTTATA+TGG + chr2.1:13684565-13684584 MS.gene35862:intron 10.0%
!! TGAAAAATTATAGAAAATGA+GGG - chr2.1:13685519-13685538 None:intergenic 15.0%
!! TTAGAAAAATGAAAATGATT+GGG - chr2.1:13684997-13685016 None:intergenic 15.0%
!! TTGAAAAATTATAGAAAATG+AGG - chr2.1:13685520-13685539 None:intergenic 15.0%
!!! ACAATTTTTCAAAACAAAAA+AGG - chr2.1:13684011-13684030 None:intergenic 15.0%
!! AAAGCTTATGAAAAAGATTA+TGG - chr2.1:13686679-13686698 None:intergenic 20.0%
!! AATAATTTCCCAAATAATCT+TGG - chr2.1:13685325-13685344 None:intergenic 20.0%
!! ACTATATACTAACCATTATT+TGG + chr2.1:13686736-13686755 MS.gene35862:intron 20.0%
!! CAACTAGTTAAAACAATAAT+GGG + chr2.1:13684522-13684541 MS.gene35862:intron 20.0%
!! CTTAAAAATCACATTATACA+TGG - chr2.1:13684801-13684820 None:intergenic 20.0%
!! CTTAGAAAAATGAAAATGAT+TGG - chr2.1:13684998-13685017 None:intergenic 20.0%
!! TATCAAACTATATGAATTCT+TGG + chr2.1:13685728-13685747 MS.gene35862:intron 20.0%
!! TCAACTAGTTAAAACAATAA+TGG + chr2.1:13684521-13684540 MS.gene35862:intron 20.0%
!! TGAAATAATGACTAGTATAT+TGG + chr2.1:13685881-13685900 MS.gene35862:intron 20.0%
!!! CTTACATGTTATTTTATGAT+TGG + chr2.1:13686622-13686641 MS.gene35862:intron 20.0%
!!! TATTTTATATGGTTTAAGCA+TGG + chr2.1:13684576-13684595 MS.gene35862:intron 20.0%
! AAAAATGAAAATGATTGGGT+AGG - chr2.1:13684993-13685012 None:intergenic 25.0%
! AAAATGAAAATGATTGGGTA+GGG - chr2.1:13684992-13685011 None:intergenic 25.0%
! AACAAAAAAGGTTATTGAGT+TGG - chr2.1:13683999-13684018 None:intergenic 25.0%
! AATGACTTAAAACACGTTTA+AGG - chr2.1:13684212-13684231 None:intergenic 25.0%
! ACATAAATTTAACCAAGGTA+TGG + chr2.1:13686196-13686215 MS.gene35862:intron 25.0%
! ACCGAAGTTAAAAAAAATCA+AGG + chr2.1:13685369-13685388 MS.gene35862:intron 25.0%
! AGGAAAAAATTTAACTCACT+TGG - chr2.1:13686364-13686383 None:intergenic 25.0%
! ATGAGATTGTTTCACAATTA+GGG - chr2.1:13683870-13683889 None:intergenic 25.0%
! ATTATCCATATCTGATATTG+CGG + chr2.1:13683673-13683692 MS.gene35862:CDS 25.0%
! ATTATGATAGAATAGAGTCA+AGG - chr2.1:13686059-13686078 None:intergenic 25.0%
! ATTCCAATTGAAAATACATC+TGG + chr2.1:13684069-13684088 MS.gene35862:CDS 25.0%
! CCAATTTAGACAATCAATAA+AGG - chr2.1:13685495-13685514 None:intergenic 25.0%
! CCGAAGTTAAAAAAAATCAA+GGG + chr2.1:13685370-13685389 MS.gene35862:intron 25.0%
! CCTCAAAATCAATATAGAAA+AGG - chr2.1:13686546-13686565 None:intergenic 25.0%
! GATACTTTCCTGAATAAATT+AGG + chr2.1:13684727-13684746 MS.gene35862:CDS 25.0%
! GCAGAAAATAAAGAAAAACT+AGG - chr2.1:13686415-13686434 None:intergenic 25.0%
! GTGTTAAAATGTGTCTAATT+TGG + chr2.1:13686436-13686455 MS.gene35862:intron 25.0%
! TGAATATGTATTAGTCACAT+TGG + chr2.1:13684655-13684674 MS.gene35862:intron 25.0%
! TGAGAATCAAGCATTTAATT+TGG - chr2.1:13684343-13684362 None:intergenic 25.0%
! TTATCATCAATTAATGCAGA+AGG - chr2.1:13686480-13686499 None:intergenic 25.0%
! TTATGATAGAATAGAGTCAA+GGG - chr2.1:13686058-13686077 None:intergenic 25.0%
! TTATTGGATCTTGGAATTAT+TGG - chr2.1:13686008-13686027 None:intergenic 25.0%
! TTTATCTGACTTCAAATGAA+AGG - chr2.1:13685048-13685067 None:intergenic 25.0%
!! AAGAGATTAATTTTTGCTCT+GGG + chr2.1:13685295-13685314 MS.gene35862:intron 25.0%
!! ATTTTATGATTGGAATTCGA+GGG + chr2.1:13686632-13686651 MS.gene35862:intron 25.0%
!! CCTTTATTGATTGTCTAAAT+TGG + chr2.1:13685492-13685511 MS.gene35862:intron 25.0%
!! TACTCAAACTTTTCAATGAA+AGG + chr2.1:13685911-13685930 MS.gene35862:intron 25.0%
!! TATTTTATGATTGGAATTCG+AGG + chr2.1:13686631-13686650 MS.gene35862:intron 25.0%
!!! AATAATGGGCATATAACAAT+AGG + chr2.1:13684536-13684555 MS.gene35862:intron 25.0%
!!! ATGTTTGGATTACTGATTTA+GGG + chr2.1:13683802-13683821 MS.gene35862:intron 25.0%
!!! CCCTTGATTTTTTTTAACTT+CGG - chr2.1:13685373-13685392 None:intergenic 25.0%
!!! CCTTTTCTATATTGATTTTG+AGG + chr2.1:13686543-13686562 MS.gene35862:CDS 25.0%
!!! GTTCTAGAAGTTTAAAGAAA+GGG - chr2.1:13685694-13685713 None:intergenic 25.0%
!!! TATGTTTGGATTACTGATTT+AGG + chr2.1:13683801-13683820 MS.gene35862:intron 25.0%
!!! TGTTCTAGAAGTTTAAAGAA+AGG - chr2.1:13685695-13685714 None:intergenic 25.0%
!!! TTGTTTTGAAAAATTGTGAC+AGG + chr2.1:13684014-13684033 MS.gene35862:intron 25.0%
AAAAATCAGGGGTTAGATAA+CGG - chr2.1:13685821-13685840 None:intergenic 30.0%
AAATGAAAATGATTGGGTAG+GGG - chr2.1:13684991-13685010 None:intergenic 30.0%
AAGTCAGATAAACTAAGCAA+TGG + chr2.1:13685056-13685075 MS.gene35862:intron 30.0%
ACGTAATGCATCTATATTAC+AGG + chr2.1:13685938-13685957 MS.gene35862:intron 30.0%
AGTAGTTAGCAAGAAAAATC+AGG - chr2.1:13685834-13685853 None:intergenic 30.0%
ATCTAATCCTATCCAACATA+AGG + chr2.1:13684315-13684334 MS.gene35862:intron 30.0%
ATGATTTACTTCTTCGTCAT+AGG + chr2.1:13684112-13684131 MS.gene35862:CDS 30.0%
ATTGTGTTTACACTGAATGA+AGG + chr2.1:13686129-13686148 MS.gene35862:CDS 30.0%
CAAAGTACGAATATCATATC+AGG + chr2.1:13683715-13683734 MS.gene35862:CDS 30.0%
CATGAGATTGTTTCACAATT+AGG - chr2.1:13683871-13683890 None:intergenic 30.0%
CTGATATGATATTCGTACTT+TGG - chr2.1:13683717-13683736 None:intergenic 30.0%
CTTCTCGAATATATAGTTGT+TGG + chr2.1:13685415-13685434 MS.gene35862:intron 30.0%
GAAGAAGTAAATCATAGTTC+CGG - chr2.1:13684107-13684126 None:intergenic 30.0%
GCAATATCAGATATGGATAA+TGG - chr2.1:13683674-13683693 None:intergenic 30.0%
GTAGTTAGCAAGAAAAATCA+GGG - chr2.1:13685833-13685852 None:intergenic 30.0%
GTCTTAGCTAAACAACATTA+TGG - chr2.1:13685667-13685686 None:intergenic 30.0%
GTCTTCATGTTTCTCATTTA+AGG + chr2.1:13684241-13684260 MS.gene35862:intron 30.0%
TAAAAGGTTATTCTGTTTGG+TGG + chr2.1:13685561-13685580 MS.gene35862:intron 30.0%
TAGTTAGCAAGAAAAATCAG+GGG - chr2.1:13685832-13685851 None:intergenic 30.0%
TCATTACAAGAATCCAACTT+TGG - chr2.1:13686709-13686728 None:intergenic 30.0%
TCCTAAAAGGTTATTCTGTT+TGG + chr2.1:13685558-13685577 MS.gene35862:intron 30.0%
TCTGGAAAATAACGAATGAT+TGG - chr2.1:13686104-13686123 None:intergenic 30.0%
TGCTAACACCTAATTTATTC+AGG - chr2.1:13684738-13684757 None:intergenic 30.0%
TTAAAAGGCTTATTGGATCT+TGG - chr2.1:13686017-13686036 None:intergenic 30.0%
TTTCACCTTAAAAGGCTTAT+TGG - chr2.1:13686024-13686043 None:intergenic 30.0%
TTTCTTGCTAACTACTCTTA+TGG + chr2.1:13685838-13685857 MS.gene35862:intron 30.0%
! AAAGGAGTTTTCACCTTAAA+AGG - chr2.1:13686032-13686051 None:intergenic 30.0%
! AAGATCCAATAAGCCTTTTA+AGG + chr2.1:13686016-13686035 MS.gene35862:CDS 30.0%
! ACCAAACAGAATAACCTTTT+AGG - chr2.1:13685562-13685581 None:intergenic 30.0%
! CAAGAGATTAATTTTTGCTC+TGG + chr2.1:13685294-13685313 MS.gene35862:intron 30.0%
! CTTTTACCAGAAACTTATTG+TGG + chr2.1:13684288-13684307 MS.gene35862:intron 30.0%
! TACATTAAGCCCATAGATTT+TGG - chr2.1:13684944-13684963 None:intergenic 30.0%
! TTTTATGATTGGAATTCGAG+GGG + chr2.1:13686633-13686652 MS.gene35862:intron 30.0%
!! AGCTTTAAATCTTCCAAAGT+TGG + chr2.1:13686693-13686712 MS.gene35862:intron 30.0%
!! TGATTTTGAAGAGGCTTTTA+AGG + chr2.1:13683894-13683913 MS.gene35862:CDS 30.0%
!! TGTTTGGATTACTGATTTAG+GGG + chr2.1:13683803-13683822 MS.gene35862:intron 30.0%
!! TTTAATTTGGTGCCTTATGT+TGG - chr2.1:13684330-13684349 None:intergenic 30.0%
AAATGTCGCAAATTCCTGTA+AGG - chr2.1:13686763-13686782 None:intergenic 35.0%
AACCATTATTTGGTCCTTAC+AGG + chr2.1:13686746-13686765 MS.gene35862:intron 35.0%
AGACAATCAATAAAGGCAGT+AGG - chr2.1:13685488-13685507 None:intergenic 35.0%
ATGCAACCACAATAAGTTTC+TGG - chr2.1:13684297-13684316 None:intergenic 35.0%
CAAACATATAATGACGAACG+TGG - chr2.1:13683789-13683808 None:intergenic 35.0%
CAATGTGCTCTGATATGAAA+AGG - chr2.1:13686384-13686403 None:intergenic 35.0%
CAATTAATGCAGAAGGATTG+AGG - chr2.1:13686473-13686492 None:intergenic 35.0%
CACGTTCGTCATTATATGTT+TGG + chr2.1:13683787-13683806 MS.gene35862:intron 35.0%
CCAAGAACAAATCTCCTAAA+AGG + chr2.1:13685545-13685564 MS.gene35862:intron 35.0%
CCAATTGAAAATACATCTGG+TGG + chr2.1:13684072-13684091 MS.gene35862:CDS 35.0%
CCCATAGATACAACCATATA+GGG + chr2.1:13686804-13686823 MS.gene35862:CDS 35.0%
CTCAACAATGTAGTTGAAGT+TGG + chr2.1:13685246-13685265 MS.gene35862:intron 35.0%
CTCTTAAACTCGACTATATG+TGG - chr2.1:13684611-13684630 None:intergenic 35.0%
GACTAGATTGTAGCTTCAAA+TGG + chr2.1:13684880-13684899 MS.gene35862:intron 35.0%
GACTTCAAATGAAAGGTAAC+AGG - chr2.1:13685041-13685060 None:intergenic 35.0%
GAGGGACATAAATTTAACCA+AGG + chr2.1:13686191-13686210 MS.gene35862:CDS 35.0%
GTCATTGTTCAATCTGTAAG+TGG + chr2.1:13684409-13684428 MS.gene35862:intron 35.0%
TCCCATAGATACAACCATAT+AGG + chr2.1:13686803-13686822 MS.gene35862:CDS 35.0%
TTCAATCTGTAAGTGGTACT+GGG + chr2.1:13684416-13684435 MS.gene35862:intron 35.0%
TTCCTGTAAGGACCAAATAA+TGG - chr2.1:13686751-13686770 None:intergenic 35.0%
! AGAAATTCTTTAGTTCCCCT+GGG + chr2.1:13685198-13685217 MS.gene35862:intron 35.0%
! ATAGAGATTTTTCATACCCC+AGG - chr2.1:13685218-13685237 None:intergenic 35.0%
! CCACATTATTCTGAGGAAAA+GGG - chr2.1:13685754-13685773 None:intergenic 35.0%
! CCACCAGATGTATTTTCAAT+TGG - chr2.1:13684075-13684094 None:intergenic 35.0%
! GGAAATACTTTTCCATACCT+TGG - chr2.1:13686211-13686230 None:intergenic 35.0%
! TAGAGATTTTTCATACCCCA+GGG - chr2.1:13685217-13685236 None:intergenic 35.0%
! TCCACATTATTCTGAGGAAA+AGG - chr2.1:13685755-13685774 None:intergenic 35.0%
!! ACGTGTTTTAAGTCATTCTC+AGG + chr2.1:13684215-13684234 MS.gene35862:CDS 35.0%
!! CCTTTTAGGAGATTTGTTCT+TGG - chr2.1:13685548-13685567 None:intergenic 35.0%
!! CTTCTAGAACATTCTTACTG+AGG + chr2.1:13685704-13685723 MS.gene35862:intron 35.0%
!! GAAAATGTTGATGATACAGC+AGG + chr2.1:13684760-13684779 MS.gene35862:CDS 35.0%
!!! TGAATTTTAAAGTGTCACGC+TGG + chr2.1:13684459-13684478 MS.gene35862:intron 35.0%
AAAGTCGCCCACAAAGAAAT+CGG + chr2.1:13686301-13686320 MS.gene35862:CDS 40.0%
ATTCGAGCTGCCAAAATCTA+TGG + chr2.1:13684931-13684950 MS.gene35862:CDS 40.0%
ATTGGAATTCGAGGGGAAAA+GGG + chr2.1:13686640-13686659 MS.gene35862:intron 40.0%
CAACTGAGCTAGACTAACAA+AGG - chr2.1:13686232-13686251 None:intergenic 40.0%
CAGGGGAACTAAAGAATTTC+TGG - chr2.1:13685199-13685218 None:intergenic 40.0%
CTAGTCTCGCATACACATTA+AGG - chr2.1:13684866-13684885 None:intergenic 40.0%
GAATAGAGTCAAGGGTCAAA+AGG - chr2.1:13686050-13686069 None:intergenic 40.0%
GAGTCATCCACATTATTCTG+AGG - chr2.1:13685761-13685780 None:intergenic 40.0%
GCTAACTACTCTTATGGCTA+TGG + chr2.1:13685844-13685863 MS.gene35862:intron 40.0%
GTGTAAACACAATGCTTGTC+TGG - chr2.1:13686122-13686141 None:intergenic 40.0%
GTTCAATCTGTAAGTGGTAC+TGG + chr2.1:13684415-13684434 MS.gene35862:intron 40.0%
TCAAATGGTCTGTACGATAC+TGG + chr2.1:13684895-13684914 MS.gene35862:CDS 40.0%
TCGTTATCTTGTGCTTGCAA+GGG + chr2.1:13685980-13685999 MS.gene35862:CDS 40.0%
TTACACTGAATGAAGGTCCT+GGG + chr2.1:13686136-13686155 MS.gene35862:CDS 40.0%
TTCGAGCTGCCAAAATCTAT+GGG + chr2.1:13684932-13684951 MS.gene35862:CDS 40.0%
TTTACACTGAATGAAGGTCC+TGG + chr2.1:13686135-13686154 MS.gene35862:CDS 40.0%
TTTGGCGGTATTTGCAATGA+GGG + chr2.1:13686173-13686192 MS.gene35862:CDS 40.0%
! AGAGATTTTTCATACCCCAG+GGG - chr2.1:13685216-13685235 None:intergenic 40.0%
! CAGAAATTCTTTAGTTCCCC+TGG + chr2.1:13685197-13685216 MS.gene35862:intron 40.0%
! CCCTTTTCCTCAGAATAATG+TGG + chr2.1:13685751-13685770 MS.gene35862:intron 40.0%
! GAAATTCTTTAGTTCCCCTG+GGG + chr2.1:13685199-13685218 MS.gene35862:intron 40.0%
! TGTTTGGTGGAAGCTCAAAA+TGG + chr2.1:13685574-13685593 MS.gene35862:intron 40.0%
! TTAGTGGCCGATTTCTTTGT+GGG - chr2.1:13686311-13686330 None:intergenic 40.0%
! TTTTGGCGGTATTTGCAATG+AGG + chr2.1:13686172-13686191 MS.gene35862:CDS 40.0%
!! CTCATGCAGTGATTTTGAAG+AGG + chr2.1:13683885-13683904 MS.gene35862:CDS 40.0%
!! TGGGAGCACCAAGATTATTT+GGG + chr2.1:13685314-13685333 MS.gene35862:intron 40.0%
!! TTACTGATTTAGGGGATACC+AGG + chr2.1:13683811-13683830 MS.gene35862:intron 40.0%
!! TTTGGTGCCTTATGTTGGAT+AGG - chr2.1:13684325-13684344 None:intergenic 40.0%
!!! CTCAATGCCTTTTTTCATGC+AGG + chr2.1:13684690-13684709 MS.gene35862:intron 40.0%
AAGTGCTCTGATGTCAGTGA+TGG + chr2.1:13685119-13685138 MS.gene35862:intron 45.0%
AGCTGCCGCAATATCAGATA+TGG - chr2.1:13683681-13683700 None:intergenic 45.0%
CGTCATAGGCTTCACATTGT+TGG + chr2.1:13684126-13684145 MS.gene35862:CDS 45.0%
GATTGGAATTCGAGGGGAAA+AGG + chr2.1:13686639-13686658 MS.gene35862:intron 45.0%
GCAAGAGCCTGCATGAAAAA+AGG - chr2.1:13684700-13684719 None:intergenic 45.0%
GGGCTTAATGTACTTGCAGA+AGG + chr2.1:13684952-13684971 MS.gene35862:CDS 45.0%
GTCGTTATCTTGTGCTTGCA+AGG + chr2.1:13685979-13685998 MS.gene35862:CDS 45.0%
GTTTCTGTGTATCTGCCAAC+TGG + chr2.1:13685793-13685812 MS.gene35862:intron 45.0%
TACACTGAATGAAGGTCCTG+GGG + chr2.1:13686137-13686156 MS.gene35862:CDS 45.0%
TGTACTTGCAGAAGGAATCC+AGG + chr2.1:13684960-13684979 MS.gene35862:CDS 45.0%
! AGGAACTCTTTTCTGACACC+AGG - chr2.1:13684192-13684211 None:intergenic 45.0%
! CTTAGTGGCCGATTTCTTTG+TGG - chr2.1:13686312-13686331 None:intergenic 45.0%
! GCGACATTTCTTCGAGTTCT+TGG + chr2.1:13686774-13686793 MS.gene35862:CDS 45.0%
!! AACCGCTTTAGAACATCTGC+AGG + chr2.1:13686591-13686610 MS.gene35862:CDS 45.0%
!! CACCTGCAGATGTTCTAAAG+CGG - chr2.1:13686596-13686615 None:intergenic 45.0%
!! CTGGGAGCACCAAGATTATT+TGG + chr2.1:13685313-13685332 MS.gene35862:intron 45.0%
!! GTTCTAAAGCGGTTTGTGCT+CGG - chr2.1:13686585-13686604 None:intergenic 45.0%
!! TGATGTCAGTGATGGACTGA+TGG + chr2.1:13685127-13685146 MS.gene35862:intron 45.0%
!! TTCTAAAGCGGTTTGTGCTC+GGG - chr2.1:13686584-13686603 None:intergenic 45.0%
!! CAATAAATATAAATAAAATA+AGG - chr2.1:13683959-13683978 None:intergenic 5.0%
!!! AAAAAAAAAAAAAATTGTTT+TGG + chr2.1:13685094-13685113 MS.gene35862:intron 5.0%
!!! CTTATTTTATTTATATTTAT+TGG + chr2.1:13683957-13683976 MS.gene35862:intron 5.0%
AAAACCTTGAACAGCACCCC+AGG - chr2.1:13686156-13686175 None:intergenic 50.0%
GAACAACACACTGCAGCTGT+AGG - chr2.1:13685463-13685482 None:intergenic 50.0%
GAGTCGTCAACGACTCTTAG+TGG - chr2.1:13686327-13686346 None:intergenic 50.0%
GATACCAGGATCATACAGCG+AGG + chr2.1:13683825-13683844 MS.gene35862:CDS 50.0%
TAGGCTTCACATTGTTGGCG+AGG + chr2.1:13684131-13684150 MS.gene35862:CDS 50.0%
TCACTTGGCAGTTCCAGTGT+TGG - chr2.1:13686349-13686368 None:intergenic 50.0%
TTCTGACACCAGGCATAGCT+AGG - chr2.1:13684182-13684201 None:intergenic 50.0%
! GATTGGGTAGGGGTTTTACC+TGG - chr2.1:13684981-13685000 None:intergenic 50.0%
! TCTAAAGCGGTTTGTGCTCG+GGG - chr2.1:13686583-13686602 None:intergenic 50.0%
! TGTGACAGGCTGTTGAGTTG+TGG + chr2.1:13684028-13684047 MS.gene35862:intron 50.0%
!! CATCTGGTGGAAGCATTCAC+CGG + chr2.1:13684085-13684104 MS.gene35862:CDS 50.0%
!! GGTTAGATAACGGAGCCAGT+TGG - chr2.1:13685811-13685830 None:intergenic 50.0%
!!! GGTGCTGTTCAAGGTTTTGG+CGG + chr2.1:13686158-13686177 MS.gene35862:CDS 50.0%
!!! TGGGGTGCTGTTCAAGGTTT+TGG + chr2.1:13686155-13686174 MS.gene35862:CDS 50.0%
CATTGTTGGCGAGGTGCAGT+TGG + chr2.1:13684140-13684159 MS.gene35862:CDS 55.0%
GACACCAGGCATAGCTAGGA+GGG - chr2.1:13684178-13684197 None:intergenic 55.0%
GCATCCTCGCTGTATGATCC+TGG - chr2.1:13683832-13683851 None:intergenic 55.0%
GTCGTTGACGACTCCAACAC+TGG + chr2.1:13686333-13686352 MS.gene35862:CDS 55.0%
TGACACCAGGCATAGCTAGG+AGG - chr2.1:13684179-13684198 None:intergenic 55.0%
! ACAGGCTGTTGAGTTGTGGC+TGG + chr2.1:13684032-13684051 MS.gene35862:intron 55.0%
!! TGATACAGCAGGTGCTGCTC+AGG + chr2.1:13684771-13684790 MS.gene35862:CDS 55.0%
CAGGCATAGCTAGGAGGGAG+AGG - chr2.1:13684173-13684192 None:intergenic 60.0%
CTCTCCCTCCTAGCTATGCC+TGG + chr2.1:13684171-13684190 MS.gene35862:CDS 60.0%
!! AGGTCCTGGGGTGCTGTTCA+AGG + chr2.1:13686149-13686168 MS.gene35862:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 13683669 13686824 13683669 ID=MS.gene35862
chr2.1 mRNA 13683669 13686824 13683669 ID=MS.gene35862.t1;Parent=MS.gene35862
chr2.1 exon 13683669 13683736 13683669 ID=MS.gene35862.t1.exon1;Parent=MS.gene35862.t1
chr2.1 CDS 13683669 13683736 13683669 ID=cds.MS.gene35862.t1;Parent=MS.gene35862.t1
chr2.1 exon 13683823 13683915 13683823 ID=MS.gene35862.t1.exon2;Parent=MS.gene35862.t1
chr2.1 CDS 13683823 13683915 13683823 ID=cds.MS.gene35862.t1;Parent=MS.gene35862.t1
chr2.1 exon 13684036 13684236 13684036 ID=MS.gene35862.t1.exon3;Parent=MS.gene35862.t1
chr2.1 CDS 13684036 13684236 13684036 ID=cds.MS.gene35862.t1;Parent=MS.gene35862.t1
chr2.1 exon 13684712 13684792 13684712 ID=MS.gene35862.t1.exon4;Parent=MS.gene35862.t1
chr2.1 CDS 13684712 13684792 13684712 ID=cds.MS.gene35862.t1;Parent=MS.gene35862.t1
chr2.1 exon 13684886 13684981 13684886 ID=MS.gene35862.t1.exon5;Parent=MS.gene35862.t1
chr2.1 CDS 13684886 13684981 13684886 ID=cds.MS.gene35862.t1;Parent=MS.gene35862.t1
chr2.1 exon 13685960 13686037 13685960 ID=MS.gene35862.t1.exon6;Parent=MS.gene35862.t1
chr2.1 CDS 13685960 13686037 13685960 ID=cds.MS.gene35862.t1;Parent=MS.gene35862.t1
chr2.1 exon 13686123 13686212 13686123 ID=MS.gene35862.t1.exon7;Parent=MS.gene35862.t1
chr2.1 CDS 13686123 13686212 13686123 ID=cds.MS.gene35862.t1;Parent=MS.gene35862.t1
chr2.1 exon 13686297 13686364 13686297 ID=MS.gene35862.t1.exon8;Parent=MS.gene35862.t1
chr2.1 CDS 13686297 13686364 13686297 ID=cds.MS.gene35862.t1;Parent=MS.gene35862.t1
chr2.1 exon 13686531 13686612 13686531 ID=MS.gene35862.t1.exon9;Parent=MS.gene35862.t1
chr2.1 CDS 13686531 13686612 13686531 ID=cds.MS.gene35862.t1;Parent=MS.gene35862.t1
chr2.1 exon 13686768 13686824 13686768 ID=MS.gene35862.t1.exon10;Parent=MS.gene35862.t1
chr2.1 CDS 13686768 13686824 13686768 ID=cds.MS.gene35862.t1;Parent=MS.gene35862.t1
Gene Sequence

>MS.gene35862

GGCCATTATCCATATCTGATATTGCGGCAGCTTCTGATGATCTTGCCAAAGTACGAATATCATATCAGGTATGATCATGATGTGTGTGATGTTGTTTGTCACTGTTTGATTTGATGTCCACGTTCGTCATTATATGTTTGGATTACTGATTTAGGGGATACCAGGATCATACAGCGAGGATGCTGCACTTAAAGCTTACCCTAATTGTGAAACAATCTCATGCAGTGATTTTGAAGAGGCTTTTAAGGTTTGATATTATAAGTTATTTCATATAATTATGTATTATTCCTTATTTTATTTATATTTATTGGCAGTGATTTTCATTTTCCAACTCAATAACCTTTTTTGTTTTGAAAAATTGTGACAGGCTGTTGAGTTGTGGCTGGCTCATAAAGTTGTCATTCCAATTGAAAATACATCTGGTGGAAGCATTCACCGGAACTATGATTTACTTCTTCGTCATAGGCTTCACATTGTTGGCGAGGTGCAGTTGGCTACTAACCTCTCCCTCCTAGCTATGCCTGGTGTCAGAAAAGAGTTCCTTAAACGTGTTTTAAGTCATTCTCAGGTCAGTCTTCATGTTTCTCATTTAAGGCTTTTTGAACATAGAACTTATATTCTTTTACCAGAAACTTATTGTGGTTGCATCTAATCCTATCCAACATAAGGCACCAAATTAAATGCTTGATTCTCATTCTAAAGTCAATGTGTCATTGTTCAATCTTTTTTTTGAATCAGCTGTCATTGTTCAATCTGTAAGTGGTACTGGGATTAATTTTCAATGTACTAATGAATTTTAAAGTGTCACGCTGGTTGAATTATTCTCTTTAAAGCTGAGCAATAATTTTTATATCAACTAGTTAAAACAATAATGGGCATATAACAATAGGCGTATAACAAGTATTTATATTTTATATGGTTTAAGCATGGACATCTTCTCCACATATAGTCGAGTTTAAGAGTGTTATATGTGTGTTGTACATAGCTGAATATGTATTAGTCACATTGGATTTGTCGAAATCTCAATGCCTTTTTTCATGCAGGCTCTTGCTTTAAGTGATACTTTCCTGAATAAATTAGGTGTTAGCAGAGAAAATGTTGATGATACAGCAGGTGCTGCTCAGGTAATCCATGTATAATGTGATTTTTAAGTTTCTCTAACGTTTTAGCACAGAATAATTTTGCTTTTGTTTTCCTTAATGTGTATGCGAGACTAGATTGTAGCTTCAAATGGTCTGTACGATACTGGTGCTATTGCAAGCATTCGAGCTGCCAAAATCTATGGGCTTAATGTACTTGCAGAAGGAATCCAGGTAAAACCCCTACCCAATCATTTTCATTTTTCTAAGAATCTTCAATTTACTTGTTTCCTGTTACCTTTCATTTGAAGTCAGATAAACTAAGCAATGGTATCAGATCTTTTCAAAAAAAAAAAAAAATTGTTTTGGCAAAGTGCTCTGATGTCAGTGATGGACTGATGGTTATAATGTAGTTGTGATCTTGTTTAAGAGTATAGAAATATTTATACCAGAAATTCTTTAGTTCCCCTGGGGTATGAAAAATCTCTATGCTTTGAGCTCAACAATGTAGTTGAAGTTGGAGACAGTGATTATTATATTACAAAGCAAGAGATTAATTTTTGCTCTGGGAGCACCAAGATTATTTGGGAAATTATTATGAATGCAGTCTTGACTCTTGACACCGAAGTTAAAAAAAATCAAGGGTGACTGATTGTGTATACTCTTTCTTCTCGAATATATAGTTGTTGGCTCGAGACAATATGTTCTTCTGCCTACAGCTGCAGTGTGTTGTTCAACCTACTGCCTTTATTGATTGTCTAAATTGGACCCTCATTTTCTATAATTTTTCAACATATCCAAGAACAAATCTCCTAAAAGGTTATTCTGTTTGGTGGAAGCTCAAAATGGTCTGTTAACAGATCTCAGATGATATACTGTCGACATTGATGCTGCAAGACAGAAATATCTTAAATTTCCATAATGTTGTTTAGCTAAGACATCACCCTTTCTTTAAACTTCTAGAACATTCTTACTGAGGCTATCAAACTATATGAATTCTTGGCCCTTTTCCTCAGAATAATGTGGATGACTCGAGACTTAAACTGTTTCTGTGTATCTGCCAACTGGCTCCGTTATCTAACCCCTGATTTTTCTTGCTAACTACTCTTATGGCTATGGATTTATTTTGTATTTGAAATAATGACTAGTATATTGGTACACACTACTCAAACTTTTCAATGAAAGGTCTAACGTAATGCATCTATATTACAGGATGATTCTGAAATCATCAGTCGTTATCTTGTGCTTGCAAGGGATCCAATAATTCCAAGATCCAATAAGCCTTTTAAGGTGAAAACTCCTTTTGACCCTTGACTCTATTCTATCATAATCACGCTTCTAGATTCTGTGTAACCAATCATTCGTTATTTTCCAGACAAGCATTGTGTTTACACTGAATGAAGGTCCTGGGGTGCTGTTCAAGGTTTTGGCGGTATTTGCAATGAGGGACATAAATTTAACCAAGGTATGGAAAAGTATTTCCTTTGTTAGTCTAGCTCAGTTGATGATAATTAAAAGATAATTTTAATAGTATGTACTATGAAATCAGATCGAAAGTCGCCCACAAAGAAATCGGCCACTAAGAGTCGTTGACGACTCCAACACTGGAACTGCCAAGTGAGTTAAATTTTTTCCTTTTCATATCAGAGCACATTGCTGTTCAACCTAGTTTTTCTTTATTTTCTGCAGTGTTAAAATGTGTCTAATTTGGATACATTTTGTCCTCAATCCTTCTGCATTAATTGATGATAAACTTAGATTTCTGAAATTATGTATTTCTCAGATACTTTGATTACCTTTTCTATATTGATTTTGAGGCATCTATGACTGATCCCCGAGCACAAACCGCTTTAGAACATCTGCAGGTGTGTTTTCTTACATGTTATTTTATGATTGGAATTCGAGGGGAAAAGGGAAAAAAAAAAACTCCATAATCTTTTTCATAAGCTTTAAATCTTCCAAAGTTGGATTCTTGTAATGAGTACTTGACTATATACTAACCATTATTTGGTCCTTACAGGAATTTGCGACATTTCTTCGAGTTCTTGGATGCTATCCCATAGATACAACCATATAG
Protein sequence

>MS.gene35862.t1

GHYPYLILRQLLMILPKYEYHIRGYQDHTARMLHLKLTLIVKQSHAVILKRLLRLLSCGWLIKLSFQLKIHLVEAFTGTMIYFFVIGFTLLARCSWLLTSPSULCLVSEKSSLNVFUVILRLLLUVILSUINUVLAEKMLMIQQVLLRLULQMVCTILVLLQAFELPKSMGLMYLQKESRMILKSSVVILCLQGIQUFQDPISLLRQALCLHUMKVLGCCSRFWRYLQUGTUIUPRSKVAHKEIGHUESLTTPTLELPNTLITFSILILRHLULIPEHKPLUNICRNLRHFFEFLDAIPUIQPY