AlfalfaGEDB | MS.gene35998

M. sativa cultivar XinJiangDaYe / MS.gene35998

KEGG: -
GO: GO:0005575 GO:0006810 GO:0008150 GO:0016021 GO:0031224 GO:0051179 GO:0051234 GO:0055085 GO:0110165
PFAM: PF03595.20

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35998.t1	RHN50927.1	93.9	605	26	2	1	595	1	604	0.00E+00	1102.4

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35998.t1	Q9FLV9	52.8	633	204	13	17	579	14	621	1.4e-163	577.8

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35998.t1	A0A396HJ49	93.9	605	26	2	1	595	1	604	0.0e+00	1102.4

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049993	MS.gene35998	-0.80162	8.26E-49	-1.69E-46
MS.gene052011	MS.gene35998	0.824737	6.86E-54	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35998.t1	MTR_6g045200	94.380	605	23	2	1	595	1	604	0.0	1157
MS.gene35998.t1	MTR_6g034030	86.851	616	56	6	1	594	1	613	0.0	1068
MS.gene35998.t1	MTR_0549s0020	95.038	524	16	1	64	577	1	524	0.0	1020
MS.gene35998.t1	MTR_6g034030	85.386	479	45	6	1	457	1	476	0.0	811
MS.gene35998.t1	MTR_4g101880	51.340	485	206	9	125	592	85	556	5.46e-160	469
MS.gene35998.t1	MTR_0549s0010	86.058	208	18	2	64	261	1	207	3.49e-119	352
MS.gene35998.t1	MTR_4g049640	43.408	311	165	4	223	523	53	362	1.39e-66	221
MS.gene35998.t1	MTR_3g112530	41.411	326	178	6	223	538	51	373	9.37e-63	212
MS.gene35998.t1	MTR_4g132400	35.077	325	201	5	208	522	32	356	9.18e-56	193

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35998.t1	AT5G24030	53.302	636	196	15	17	579	14	621	0.0	618
MS.gene35998.t1	AT4G27970	59.877	486	163	8	108	582	41	505	0.0	567
MS.gene35998.t1	AT4G27970	59.877	486	163	8	108	582	41	505	0.0	567
MS.gene35998.t1	AT5G24030	52.783	521	149	13	17	467	14	507	7.41e-169	490
MS.gene35998.t1	AT4G27970	64.305	367	127	3	225	590	1	364	1.39e-164	474
MS.gene35998.t1	AT4G27970	64.305	367	127	3	225	590	1	364	1.39e-164	474
MS.gene35998.t1	AT4G27970	63.435	361	104	5	108	458	41	383	1.87e-155	451
MS.gene35998.t1	AT4G27970	63.435	361	104	5	108	458	41	383	1.87e-155	451
MS.gene35998.t1	AT1G12480	55.294	425	169	5	146	549	102	526	1.26e-154	456
MS.gene35998.t1	AT1G62280	39.542	306	176	6	223	520	46	350	1.06e-68	228

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Find 116 sgRNAs with CRISPR-Local

Find 187 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCTACATCAATCTCTA+TGG	0.381498	6.2:+24168561	MS.gene35998:CDS
AACAATGGCCGTTTCTTCTC+CGG	0.397146	6.2:+24168240	MS.gene35998:CDS
AACACTGGATGTAGCTCTTT+AGG	0.372096	6.2:-24168839	None:intergenic
AACCCTAACCAGTGTTTATG+TGG	0.454199	6.2:-24169608	None:intergenic
AACGGCCATTGTTTGTCATG+AGG	0.606151	6.2:-24168230	None:intergenic
AACTTACCAATGAGAAATAA+AGG	0.395497	6.2:-24168964	None:intergenic
AACTTTGTTGGAGCGTTACT+TGG	0.463926	6.2:+24168704	MS.gene35998:CDS
AAGAAGAAGTTAACACGGAT+TGG	0.491885	6.2:-24168482	None:intergenic
AAGCCACATAAACACTGGTT+AGG	0.404899	6.2:+24169605	MS.gene35998:CDS
AAGTCTCAACCAATGCCAAA+AGG	0.547948	6.2:+24167918	MS.gene35998:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAACCTACATCAATCTCTA+TGG	+	chr6.2:24168561-24168580	MS.gene35998:CDS	30.0%
ATCTCTATGGTACATTCTTA+TGG	+	chr6.2:24168574-24168593	MS.gene35998:CDS	30.0%
CTTAAAAAGGAGTCTTACTT+TGG	-	chr6.2:24167214-24167233	None:intergenic	30.0%
GACTTAAGAGTTTCTAATTC+TGG	-	chr6.2:24168191-24168210	None:intergenic	30.0%
GAGACTGTTTGTATTACTTA+CGG	+	chr6.2:24167307-24167326	MS.gene35998:intron	30.0%
TCCAAATCATGAACATAAAG+AGG	-	chr6.2:24167289-24167308	None:intergenic	30.0%
TCCTCTTTATGTTCATGATT+TGG	+	chr6.2:24167285-24167304	MS.gene35998:intron	30.0%
TTAAGCTCGAGACATAATAT+TGG	-	chr6.2:24168602-24168621	None:intergenic	30.0%
AAAAACAAGAGAGCTATCCA+CGG	-	chr6.2:24168512-24168531	None:intergenic	35.0%
AAGAAGAAGTTAACACGGAT+TGG	-	chr6.2:24168485-24168504	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6.2	gene	24167078	24169760	24167078	ID=MS.gene35998
chr6.2	mRNA	24167078	24169760	24167078	ID=MS.gene35998.t1;Parent=MS.gene35998
chr6.2	exon	24167078	24167215	24167078	ID=MS.gene35998.t1.exon1;Parent=MS.gene35998.t1
chr6.2	CDS	24167078	24167215	24167078	ID=cds.MS.gene35998.t1;Parent=MS.gene35998.t1
chr6.2	exon	24167720	24168185	24167720	ID=MS.gene35998.t1.exon2;Parent=MS.gene35998.t1
chr6.2	CDS	24167720	24168185	24167720	ID=cds.MS.gene35998.t1;Parent=MS.gene35998.t1
chr6.2	exon	24168216	24168979	24168216	ID=MS.gene35998.t1.exon3;Parent=MS.gene35998.t1
chr6.2	CDS	24168216	24168979	24168216	ID=cds.MS.gene35998.t1;Parent=MS.gene35998.t1
chr6.2	exon	24169237	24169268	24169237	ID=MS.gene35998.t1.exon4;Parent=MS.gene35998.t1
chr6.2	CDS	24169237	24169268	24169237	ID=cds.MS.gene35998.t1;Parent=MS.gene35998.t1
chr6.2	exon	24169373	24169760	24169373	ID=MS.gene35998.t1.exon5;Parent=MS.gene35998.t1
chr6.2	CDS	24169373	24169760	24169373	ID=cds.MS.gene35998.t1;Parent=MS.gene35998.t1

Gene Sequence

>MS.gene35998

ATGGAAAATCACATAACTCTTGAAATCACAGAACAAGCTTCTCCTCCAGAAACTCCATCACTAATAAAGTATATATCATCAAATGAACTTGAAGATTTTGATTCATTTGAATCTCAGTTTCCAAGCCCAATTTCCAAAGTAAGACTCCTTTTTAAGTCATTTTTATTGCTTATGATAAAATTTAAAGTGCCTATATCATTTTAGAATTCCTCTTTATGTTCATGATTTGGAGACTGTTTGTATTACTTACGGAAACAACTTACGACATGTCCATAAATGCTTGAGGATAGCTTATGAAAACAGCTTATATATTGCATAAAAATTGTTACTTGATTTTATCTTTTGTTATAGAAATATGGTATGTTTCAATTGGTTTATTTTGACTTATTTCCTGACATAAGCACTTGTGAGACTGTTTGAGGCAATTTATATAAACAACTTATGACATGTTCATAAATTAATTTCTTCTTATTTCATTAGCCTCTTCAAAATAGCTTATGAAACCAACTTATTTTATCTTTTGTTATAGAATTATCTTATATATAAGCACTTATATGTAAGCGCTTAATTAAGGTCTTTATCCAAACAAGACCTTGTGACATAACTAAAATCTAACTTTTTTTTTCCATGTTTTTTCAATAGGAAAGTGAAGAAACATACAATCATCAAAGAAAACCTTCAATGTCTGTCAGCATGCCACTTTGTTATAAAGAAACTCAACTTCAATCACTAAACATCAACAATACCAAGAATGTTTCCTTTAGTGGAGAAAATGTTATTATCAAAGATGATTTTCCATCAGGAATTGCAATGTCTGAGCCTCCAAGACAACCAAAATTCAAGTCTCAACCAATGCCAAAAGGAGTTACTTACCAACAAGATGGTTCTCAAACTCGAAAGGCTAATCGTAATTATAATCAACCTGGAATAAAAATGTTTAGAGATAAGAGATTTGACTCGTTCAAAACATGGTCTGGTGGAGGACTTGAGAGACAATTATCGATTCTACGTGGAAAAGAACCGAGTGGAAATGCACAAGATGGTAACAACGCTACAAGAAGCTTTGATAGGGCATTACCTGTTGATAGGTATTTTGATGCATTGGAAGGTCCAGAATTAGAAACTCTTAAGTCATCAGAAGAGATAATGCTACCTCATGACAAACAATGGCCGTTTCTTCTCCGGTTCCCCGTTTCATCGTTCGGTATATGCCTCGGAGTTAGCAGCCAAGCAATTCTTTGGAAAACCCTAGCCACATCGCCTACCACCGAATTCCTTCACATAACTCCCAAAATAAACCTAATCTTATGGTACATCTCCGCGGTACTTATAGCTACCATTTTCGCTGTCTATATTCTCAAACTCCTTTTCTACTTCGAAGCAGTTCGTCGGGAATACTACCATCCAATCCGTGTTAACTTCTTCTTCGCACCGTGGATAGCTCTCTTGTTTTTAGCACTAGGAGTTCCTCCATCCGTAACCAAAAACCTACATCAATCTCTATGGTACATTCTTATGGCACCAATATTATGTCTCGAGCTTAAGATTTACGGACAATGGATGTCCGGTGGACAAAGGCGGCTTTCGAAGGTGGCGAACCCTTCAAATCATTTATCAGTTGTTGGAAACTTTGTTGGAGCGTTACTTGGTGCATCGATGGGACTAATTGAAGGACCAATTTTCTTCTTTGCTGTTGGACTTGCTCATTACACTGTGTTATTTGTGACACTTTATCAAAGACTTCCAACCAATGCAACACTACCTAAAGAGCTACATCCAGTGTTCTTTCTCTTTGTTGCAGCTCCTAGTGTTGCTTCAATGGCTTGGGCTAAGATTCAAGGTTCTTTTGATTATGGATCAAGGATTGCTTATTTCATTGCATTGTTCCTTTATTTCTCATTGGTAAGTTTTATTGTCACACTTTTATTTGAAATAATCATAATTAGTTATCAATTTAATATCTGATATATAAGCCAATTGTCCACGCGATATTAAATTTATTTTTTGAGGGGGATAGCCCACCACAATAGCTTGCTATTGGGTCTCTCAAGTGTCTCCTTTGCGATGAACTATTGCATTGACCTGGCGGGCCCCACCAAAAAAATATGCATAATTGTTTATCATTGAAATTTACTCACTGGTTTTATATGCTTCTACAGGCTGTCAGGATCAATTTCTTCAGAGGATTTAAGTAAGTTACATTAAATCTCCCCTATCTAGTTATATTATTTAATTAATTTCTCAATACTGTTGAATTTTCTTTCATAAATTAATTTTTTTATGTTATTTTTACAGATTTTCTCTTGCATGGTGGGCCTATACTTTTCCAATGACTGGTGCAGCAATTGCAACAATAAGATACTCAAATCAAGTACCAAATATTGTCACTAAGTCATTGTGTGTTGCATTGGCTCTAATCTCAACATTCACGGTTATGGCACTTCTTCTATCAACAATATTGCATGCATTTGTCTTTAGAGATCTCTTCCCTAATGACATTGCTATTGCCATTAGTGATAGAAGGAGAAAGCCACATAAACACTGGTTAGGGTTCAGATATGGAAGCCAAGACTCCAAAGAGATTGAAAATTACTTGAAGTTTGTGAACACTGATGATAGTAGTTTGGATGATTCTACGCCACAACCGTCCTCAAACGATGCCGATCAAAATTTGCCAAAATAA

Protein sequence

>MS.gene35998.t1

MENHITLEITEQASPPETPSLIKYISSNELEDFDSFESQFPSPISKESEETYNHQRKPSMSVSMPLCYKETQLQSLNINNTKNVSFSGENVIIKDDFPSGIAMSEPPRQPKFKSQPMPKGVTYQQDGSQTRKANRNYNQPGIKMFRDKRFDSFKTWSGGGLERQLSILRGKEPSGNAQDGNNATRSFDRALPVDRYFDALEEEIMLPHDKQWPFLLRFPVSSFGICLGVSSQAILWKTLATSPTTEFLHITPKINLILWYISAVLIATIFAVYILKLLFYFEAVRREYYHPIRVNFFFAPWIALLFLALGVPPSVTKNLHQSLWYILMAPILCLELKIYGQWMSGGQRRLSKVANPSNHLSVVGNFVGALLGASMGLIEGPIFFFAVGLAHYTVLFVTLYQRLPTNATLPKELHPVFFLFVAAPSVASMAWAKIQGSFDYGSRIAYFIALFLYFSLAVRINFFRGFKFSLAWWAYTFPMTGAAIATIRYSNQVPNIVTKSLCVALALISTFTVMALLLSTILHAFVFRDLFPNDIAIAISDRRRKPHKHWLGFRYGSQDSKEIENYLKFVNTDDSSLDDSTPQPSSNDADQNLPK