Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36173.t1 | XP_003622535.1 | 86.9 | 145 | 18 | 1 | 1 | 144 | 1 | 145 | 7.00E-68 | 266.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36173.t1 | P62927 | 42.0 | 131 | 69 | 5 | 1 | 127 | 1 | 128 | 4.8e-17 | 89.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36173.t1 | G7KTY0 | 86.9 | 145 | 18 | 1 | 1 | 144 | 1 | 145 | 5.0e-68 | 266.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051996 | MS.gene36173 | 0.800484 | 1.41E-48 | -1.69E-46 |
| MS.gene056920 | MS.gene36173 | 0.801948 | 7.07E-49 | -1.69E-46 |
| MS.gene056923 | MS.gene36173 | 0.806333 | 8.66E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36173.t1 | MTR_7g044980 | 86.897 | 145 | 18 | 1 | 1 | 144 | 1 | 145 | 1.03e-90 | 259 |
| MS.gene36173.t1 | MTR_7g044920 | 56.579 | 152 | 58 | 4 | 1 | 144 | 1 | 152 | 1.65e-51 | 160 |
| MS.gene36173.t1 | MTR_8g056800 | 67.708 | 96 | 29 | 2 | 50 | 144 | 7 | 101 | 1.52e-40 | 131 |
| MS.gene36173.t1 | MTR_3g436100 | 45.161 | 155 | 73 | 7 | 1 | 144 | 1 | 154 | 1.43e-34 | 118 |
| MS.gene36173.t1 | MTR_8g022430 | 45.000 | 140 | 69 | 7 | 1 | 132 | 1 | 140 | 1.71e-31 | 109 |
| MS.gene36173.t1 | MTR_6g017170 | 52.419 | 124 | 50 | 7 | 1 | 116 | 1 | 123 | 7.12e-31 | 107 |
| MS.gene36173.t1 | MTR_8g022420 | 51.145 | 131 | 56 | 7 | 1 | 124 | 1 | 130 | 9.19e-31 | 108 |
| MS.gene36173.t1 | MTR_8g022400 | 48.855 | 131 | 60 | 5 | 1 | 124 | 1 | 131 | 2.27e-30 | 107 |
| MS.gene36173.t1 | MTR_3g067270 | 47.287 | 129 | 63 | 4 | 1 | 124 | 1 | 129 | 6.51e-30 | 105 |
| MS.gene36173.t1 | MTR_3g467750 | 47.287 | 129 | 63 | 4 | 1 | 124 | 1 | 129 | 6.51e-30 | 105 |
| MS.gene36173.t1 | MTR_6g017150 | 50.758 | 132 | 57 | 7 | 1 | 124 | 1 | 132 | 1.29e-29 | 105 |
| MS.gene36173.t1 | MTR_3g463570 | 48.855 | 131 | 60 | 6 | 1 | 124 | 1 | 131 | 1.32e-29 | 105 |
| MS.gene36173.t1 | MTR_8g461020 | 45.865 | 133 | 63 | 5 | 3 | 127 | 4 | 135 | 4.43e-29 | 103 |
| MS.gene36173.t1 | MTR_3g067280 | 46.512 | 129 | 64 | 4 | 1 | 124 | 1 | 129 | 9.22e-29 | 102 |
| MS.gene36173.t1 | MTR_3g067437 | 47.287 | 129 | 62 | 4 | 1 | 123 | 1 | 129 | 1.18e-28 | 102 |
| MS.gene36173.t1 | MTR_6g036620 | 45.517 | 145 | 61 | 7 | 1 | 140 | 1 | 132 | 3.17e-27 | 99.0 |
| MS.gene36173.t1 | MTR_7g056817 | 47.761 | 134 | 60 | 6 | 1 | 124 | 1 | 134 | 1.98e-26 | 97.1 |
| MS.gene36173.t1 | MTR_4g026590 | 41.401 | 157 | 67 | 6 | 1 | 144 | 1 | 145 | 6.11e-25 | 93.2 |
| MS.gene36173.t1 | MTR_6g047880 | 43.939 | 132 | 64 | 7 | 1 | 123 | 1 | 131 | 1.48e-24 | 92.0 |
| MS.gene36173.t1 | MTR_3g463570 | 43.307 | 127 | 67 | 4 | 3 | 124 | 25 | 151 | 1.71e-24 | 92.4 |
| MS.gene36173.t1 | MTR_6g047900 | 45.865 | 133 | 59 | 8 | 1 | 124 | 1 | 129 | 2.35e-23 | 89.0 |
| MS.gene36173.t1 | MTR_7g056803 | 47.368 | 133 | 61 | 7 | 1 | 124 | 1 | 133 | 1.07e-22 | 87.4 |
| MS.gene36173.t1 | MTR_3g067510 | 46.218 | 119 | 49 | 5 | 1 | 108 | 1 | 115 | 3.33e-22 | 85.9 |
| MS.gene36173.t1 | MTR_3g067430 | 40.000 | 135 | 70 | 5 | 1 | 124 | 1 | 135 | 8.07e-22 | 85.1 |
| MS.gene36173.t1 | MTR_3g067570 | 47.826 | 92 | 42 | 3 | 21 | 108 | 1 | 90 | 9.75e-22 | 83.6 |
| MS.gene36173.t1 | MTR_3g467760 | 41.732 | 127 | 49 | 5 | 1 | 124 | 1 | 105 | 9.94e-22 | 84.0 |
| MS.gene36173.t1 | MTR_3g067510 | 44.355 | 124 | 49 | 5 | 1 | 108 | 1 | 120 | 2.51e-21 | 84.0 |
| MS.gene36173.t1 | MTR_3g067500 | 39.823 | 113 | 64 | 3 | 21 | 129 | 1 | 113 | 1.02e-20 | 81.6 |
| MS.gene36173.t1 | MTR_3g067580 | 41.221 | 131 | 70 | 5 | 1 | 124 | 9 | 139 | 1.99e-20 | 81.6 |
| MS.gene36173.t1 | MTR_3g067540 | 43.382 | 136 | 64 | 8 | 1 | 124 | 1 | 135 | 4.22e-20 | 80.9 |
| MS.gene36173.t1 | MTR_3g067555 | 43.382 | 136 | 64 | 8 | 1 | 124 | 1 | 135 | 4.22e-20 | 80.9 |
| MS.gene36173.t1 | MTR_3g067445 | 41.880 | 117 | 58 | 4 | 1 | 107 | 1 | 117 | 1.43e-19 | 79.0 |
| MS.gene36173.t1 | MTR_1g019650 | 44.828 | 116 | 52 | 7 | 1 | 113 | 1 | 107 | 5.14e-19 | 77.0 |
| MS.gene36173.t1 | MTR_7g029540 | 39.535 | 129 | 53 | 7 | 1 | 113 | 1 | 120 | 7.91e-18 | 74.3 |
| MS.gene36173.t1 | MTR_3g067535 | 43.382 | 136 | 60 | 10 | 1 | 124 | 1 | 131 | 2.94e-17 | 73.2 |
| MS.gene36173.t1 | MTR_3g067555 | 39.837 | 123 | 62 | 7 | 13 | 124 | 1 | 122 | 4.34e-15 | 67.4 |
| MS.gene36173.t1 | MTR_3g067550 | 40.000 | 115 | 57 | 7 | 21 | 124 | 1 | 114 | 3.69e-13 | 62.4 |
| MS.gene36173.t1 | MTR_3g067555 | 40.000 | 115 | 57 | 7 | 21 | 124 | 1 | 114 | 3.69e-13 | 62.4 |
| MS.gene36173.t1 | MTR_6g082060 | 35.484 | 155 | 76 | 6 | 1 | 140 | 1 | 146 | 1.12e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 41 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAGAATCCATTTCCATTAA+TGG | 0.138065 | 7.1:-54957502 | None:intergenic |
| CCATGTTCGATTTCAGAATT+AGG | 0.219721 | 7.1:-54957387 | None:intergenic |
| ATCCCACATGGATTGCATTA+AGG | 0.252731 | 7.1:+54957338 | MS.gene36173:CDS |
| AAGAATCCATTTCCATTAAT+GGG | 0.254802 | 7.1:-54957501 | None:intergenic |
| ACTGTATCTTACCCCATTAA+TGG | 0.304225 | 7.1:+54957489 | MS.gene36173:CDS |
| TCCCACATGGATTGCATTAA+GGG | 0.338204 | 7.1:+54957339 | MS.gene36173:CDS |
| GGCTTATGTTAAGCTTGCTT+TGG | 0.344621 | 7.1:+54956782 | MS.gene36173:CDS |
| TCTTACCCCATTAATGGAAA+TGG | 0.347113 | 7.1:+54957495 | MS.gene36173:CDS |
| TGCCTCGCGTGGGGAATAAT+TGG | 0.352167 | 7.1:+54957252 | MS.gene36173:CDS |
| ATGGATTGCATTAAGGGAAA+TGG | 0.362237 | 7.1:+54957345 | MS.gene36173:CDS |
| CATGTGGGATTGACATAAGT+TGG | 0.416016 | 7.1:-54957325 | None:intergenic |
| GCGTTCGGCTTCAACGTTAG+AGG | 0.426857 | 7.1:-54957421 | None:intergenic |
| CCACGCGAGGCAACCACATC+CGG | 0.431307 | 7.1:-54957241 | None:intergenic |
| CATGCTTCTACCTTCTTCAT+CGG | 0.451053 | 7.1:-54957174 | None:intergenic |
| ATGCTTCTACCTTCTTCATC+GGG | 0.462775 | 7.1:-54957173 | None:intergenic |
| TGGATTGCATTAAGGGAAAT+GGG | 0.464127 | 7.1:+54957346 | MS.gene36173:CDS |
| GATTAAGTGGCAATCTCTAC+AGG | 0.474451 | 7.1:-54957531 | None:intergenic |
| CCGGATGTGGTTGCCTCGCG+TGG | 0.488380 | 7.1:+54957241 | MS.gene36173:CDS |
| GAGAATCTTGAAGTAGCGTT+CGG | 0.495476 | 7.1:-54957436 | None:intergenic |
| ATAGACATATCAAGATTAAG+TGG | 0.519324 | 7.1:-54957544 | None:intergenic |
| ACAGTATGTGGCTCCGGATG+TGG | 0.521366 | 7.1:+54957228 | MS.gene36173:CDS |
| CGGATGTGGTTGCCTCGCGT+GGG | 0.521966 | 7.1:+54957242 | MS.gene36173:CDS |
| TCAGTTCATACAACAGTATG+TGG | 0.525112 | 7.1:+54957216 | MS.gene36173:CDS |
| CATACAACAGTATGTGGCTC+CGG | 0.542165 | 7.1:+54957222 | MS.gene36173:CDS |
| ACGACCATCTCTTACGAAGA+TGG | 0.542973 | 7.1:+54957290 | MS.gene36173:CDS |
| TCGACCATCTTCGTAAGAGA+TGG | 0.555256 | 7.1:-54957294 | None:intergenic |
| AGTCATAAGCCCGATGAAGA+AGG | 0.556957 | 7.1:+54957164 | MS.gene36173:intron |
| CTCGCGTGGGGAATAATTGG+TGG | 0.561996 | 7.1:+54957255 | MS.gene36173:CDS |
| CCTAATTCTGAAATCGAACA+TGG | 0.569253 | 7.1:+54957387 | MS.gene36173:CDS |
| ATTCTGAAATCGAACATGGC+TGG | 0.571433 | 7.1:+54957391 | MS.gene36173:CDS |
| ATGTGGGATTGACATAAGTT+GGG | 0.572370 | 7.1:-54957324 | None:intergenic |
| TCCCTTAATGCAATCCATGT+GGG | 0.582118 | 7.1:-54957340 | None:intergenic |
| AGAATCCATTTCCATTAATG+GGG | 0.584366 | 7.1:-54957500 | None:intergenic |
| GGATGTGGTTGCCTCGCGTG+GGG | 0.586874 | 7.1:+54957243 | MS.gene36173:CDS |
| TAGAAGCATGTGACACAACA+TGG | 0.603510 | 7.1:+54957187 | MS.gene36173:CDS |
| CAACTTATGTCAATCCCACA+TGG | 0.603760 | 7.1:+54957326 | MS.gene36173:CDS |
| TTCCCTTAATGCAATCCATG+TGG | 0.622632 | 7.1:-54957341 | None:intergenic |
| ACAAATTGTTGCAAGAAAGA+CGG | 0.629650 | 7.1:-54956808 | None:intergenic |
| CACCAATTATTCCCCACGCG+AGG | 0.633688 | 7.1:-54957254 | None:intergenic |
| TGGGGTAAGATACAGTCTTG+AGG | 0.662338 | 7.1:-54957482 | None:intergenic |
| ATCTTCGTAAGAGATGGTCG+TGG | 0.691138 | 7.1:-54957288 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACTAAGTATATAACTTATTT+AGG | + | chr7.1:54957021-54957040 | MS.gene36173:intron | 15.0% |
| !!! | TTTTATGTTAGAAAACTCAT+GGG | + | chr7.1:54956876-54956895 | MS.gene36173:intron | 20.0% |
| !!! | TTTTTATGTTAGAAAACTCA+TGG | + | chr7.1:54956875-54956894 | MS.gene36173:intron | 20.0% |
| ! | AAGAATCCATTTCCATTAAT+GGG | - | chr7.1:54957504-54957523 | None:intergenic | 25.0% |
| ! | CTAACATAAAAAGAAATGCA+TGG | - | chr7.1:54956867-54956886 | None:intergenic | 25.0% |
| ACAAATTGTTGCAAGAAAGA+CGG | - | chr7.1:54956811-54956830 | None:intergenic | 30.0% | |
| AGAATCCATTTCCATTAATG+GGG | - | chr7.1:54957503-54957522 | None:intergenic | 30.0% | |
| CAAGAATCCATTTCCATTAA+TGG | - | chr7.1:54957505-54957524 | None:intergenic | 30.0% | |
| ACTGTATCTTACCCCATTAA+TGG | + | chr7.1:54957489-54957508 | MS.gene36173:CDS | 35.0% | |
| ATGGGAAAGTCAGTTAAAGT+TGG | - | chr7.1:54957140-54957159 | None:intergenic | 35.0% | |
| ATGTGGGATTGACATAAGTT+GGG | - | chr7.1:54957327-54957346 | None:intergenic | 35.0% | |
| CCATGTTCGATTTCAGAATT+AGG | - | chr7.1:54957390-54957409 | None:intergenic | 35.0% | |
| GAAAGTCAGTTAAAGTTGGA+AGG | - | chr7.1:54957136-54957155 | None:intergenic | 35.0% | |
| TCAGTTCATACAACAGTATG+TGG | + | chr7.1:54957216-54957235 | MS.gene36173:CDS | 35.0% | |
| TCTTACCCCATTAATGGAAA+TGG | + | chr7.1:54957495-54957514 | MS.gene36173:CDS | 35.0% | |
| TGGATTGCATTAAGGGAAAT+GGG | + | chr7.1:54957346-54957365 | MS.gene36173:CDS | 35.0% | |
| ! | ATGGATTGCATTAAGGGAAA+TGG | + | chr7.1:54957345-54957364 | MS.gene36173:CDS | 35.0% |
| !! | CCTAATTCTGAAATCGAACA+TGG | + | chr7.1:54957387-54957406 | MS.gene36173:CDS | 35.0% |
| ATCGGGCTTATGACTGTAAA+TGG | - | chr7.1:54957159-54957178 | None:intergenic | 40.0% | |
| ATGCTTCTACCTTCTTCATC+GGG | - | chr7.1:54957176-54957195 | None:intergenic | 40.0% | |
| CAACTTATGTCAATCCCACA+TGG | + | chr7.1:54957326-54957345 | MS.gene36173:CDS | 40.0% | |
| CATGCTTCTACCTTCTTCAT+CGG | - | chr7.1:54957177-54957196 | None:intergenic | 40.0% | |
| CATGTGGGATTGACATAAGT+TGG | - | chr7.1:54957328-54957347 | None:intergenic | 40.0% | |
| GAGAATCTTGAAGTAGCGTT+CGG | - | chr7.1:54957439-54957458 | None:intergenic | 40.0% | |
| TCCCTTAATGCAATCCATGT+GGG | - | chr7.1:54957343-54957362 | None:intergenic | 40.0% | |
| TCGGGCTTATGACTGTAAAT+GGG | - | chr7.1:54957158-54957177 | None:intergenic | 40.0% | |
| TTCCCTTAATGCAATCCATG+TGG | - | chr7.1:54957344-54957363 | None:intergenic | 40.0% | |
| ! | ATCCCACATGGATTGCATTA+AGG | + | chr7.1:54957338-54957357 | MS.gene36173:CDS | 40.0% |
| ! | GGCTTATGTTAAGCTTGCTT+TGG | + | chr7.1:54956782-54956801 | MS.gene36173:CDS | 40.0% |
| ! | TAGAAGCATGTGACACAACA+TGG | + | chr7.1:54957187-54957206 | MS.gene36173:CDS | 40.0% |
| ! | TCCCACATGGATTGCATTAA+GGG | + | chr7.1:54957339-54957358 | MS.gene36173:CDS | 40.0% |
| !! | ATTCTGAAATCGAACATGGC+TGG | + | chr7.1:54957391-54957410 | MS.gene36173:CDS | 40.0% |
| ACGACCATCTCTTACGAAGA+TGG | + | chr7.1:54957290-54957309 | MS.gene36173:CDS | 45.0% | |
| AGTCATAAGCCCGATGAAGA+AGG | + | chr7.1:54957164-54957183 | MS.gene36173:intron | 45.0% | |
| ATCTTCGTAAGAGATGGTCG+TGG | - | chr7.1:54957291-54957310 | None:intergenic | 45.0% | |
| CATACAACAGTATGTGGCTC+CGG | + | chr7.1:54957222-54957241 | MS.gene36173:CDS | 45.0% | |
| TCGACCATCTTCGTAAGAGA+TGG | - | chr7.1:54957297-54957316 | None:intergenic | 45.0% | |
| TGGGGTAAGATACAGTCTTG+AGG | - | chr7.1:54957485-54957504 | None:intergenic | 45.0% | |
| !! | AATAAAAATTATATTAATAC+TGG | - | chr7.1:54957082-54957101 | None:intergenic | 5.0% |
| ACAGTATGTGGCTCCGGATG+TGG | + | chr7.1:54957228-54957247 | MS.gene36173:CDS | 55.0% | |
| CACCAATTATTCCCCACGCG+AGG | - | chr7.1:54957257-54957276 | None:intergenic | 55.0% | |
| CTCGCGTGGGGAATAATTGG+TGG | + | chr7.1:54957255-54957274 | MS.gene36173:CDS | 55.0% | |
| GCGTTCGGCTTCAACGTTAG+AGG | - | chr7.1:54957424-54957443 | None:intergenic | 55.0% | |
| TGCCTCGCGTGGGGAATAAT+TGG | + | chr7.1:54957252-54957271 | MS.gene36173:CDS | 55.0% | |
| CCACGCGAGGCAACCACATC+CGG | - | chr7.1:54957244-54957263 | None:intergenic | 65.0% | |
| CGGATGTGGTTGCCTCGCGT+GGG | + | chr7.1:54957242-54957261 | MS.gene36173:CDS | 65.0% | |
| GGATGTGGTTGCCTCGCGTG+GGG | + | chr7.1:54957243-54957262 | MS.gene36173:CDS | 65.0% | |
| CCGGATGTGGTTGCCTCGCG+TGG | + | chr7.1:54957241-54957260 | MS.gene36173:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 54956780 | 54957551 | 54956780 | ID=MS.gene36173 |
| chr7.1 | mRNA | 54956780 | 54957551 | 54956780 | ID=MS.gene36173.t1;Parent=MS.gene36173 |
| chr7.1 | exon | 54956780 | 54956828 | 54956780 | ID=MS.gene36173.t1.exon1;Parent=MS.gene36173.t1 |
| chr7.1 | CDS | 54956780 | 54956828 | 54956780 | ID=cds.MS.gene36173.t1;Parent=MS.gene36173.t1 |
| chr7.1 | exon | 54957166 | 54957551 | 54957166 | ID=MS.gene36173.t1.exon2;Parent=MS.gene36173.t1 |
| chr7.1 | CDS | 54957166 | 54957551 | 54957166 | ID=cds.MS.gene36173.t1;Parent=MS.gene36173.t1 |
| Gene Sequence |
| Protein sequence |
