Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056923.t1 | XP_013467946.1 | 96.9 | 194 | 6 | 0 | 1 | 194 | 1 | 194 | 5.50E-92 | 347.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056923.t1 | P0DI40 | 84.5 | 194 | 28 | 1 | 1 | 194 | 1 | 192 | 5.2e-83 | 308.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056923.t1 | A0A072VKN9 | 96.9 | 194 | 6 | 0 | 1 | 194 | 1 | 194 | 3.9e-92 | 347.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049549 | MS.gene056923 | 0.807164 | 5.78E-50 | -1.69E-46 |
| MS.gene051996 | MS.gene056923 | 0.859401 | 4.24E-63 | -1.69E-46 |
| MS.gene052223 | MS.gene056923 | 0.851266 | 9.93E-61 | -1.69E-46 |
| MS.gene05240 | MS.gene056923 | 0.820356 | 7.16E-53 | -1.69E-46 |
| MS.gene052706 | MS.gene056923 | 0.801183 | 1.02E-48 | -1.69E-46 |
| MS.gene054138 | MS.gene056923 | 0.812999 | 3.20E-51 | -1.69E-46 |
| MS.gene054277 | MS.gene056923 | 0.847055 | 1.47E-59 | -1.69E-46 |
| MS.gene054742 | MS.gene056923 | 0.857928 | 1.17E-62 | -1.69E-46 |
| MS.gene056330 | MS.gene056923 | 0.858458 | 8.12E-63 | -1.69E-46 |
| MS.gene056691 | MS.gene056923 | 0.821381 | 4.16E-53 | -1.69E-46 |
| MS.gene056920 | MS.gene056923 | 0.883738 | 3.48E-71 | -1.69E-46 |
| MS.gene056922 | MS.gene056923 | 0.864502 | 1.16E-64 | -1.69E-46 |
| MS.gene056923 | MS.gene056924 | 0.960468 | 1.37E-118 | -1.69E-46 |
| MS.gene056923 | MS.gene056925 | 0.945801 | 1.54E-104 | -1.69E-46 |
| MS.gene056923 | MS.gene057261 | 0.814964 | 1.18E-51 | -1.69E-46 |
| MS.gene056923 | MS.gene059328 | 0.818494 | 1.90E-52 | -1.69E-46 |
| MS.gene056923 | MS.gene060087 | 0.830674 | 2.57E-55 | -1.69E-46 |
| MS.gene056923 | MS.gene062651 | 0.811027 | 8.60E-51 | -1.69E-46 |
| MS.gene056923 | MS.gene063988 | 0.856472 | 3.14E-62 | -1.69E-46 |
| MS.gene056923 | MS.gene064237 | -0.818573 | 1.83E-52 | -1.69E-46 |
| MS.gene056923 | MS.gene064807 | 0.807358 | 5.26E-50 | -1.69E-46 |
| MS.gene056923 | MS.gene065724 | 0.868258 | 7.46E-66 | -1.69E-46 |
| MS.gene056923 | MS.gene065728 | 0.837715 | 4.41E-57 | -1.69E-46 |
| MS.gene056923 | MS.gene065729 | 0.954896 | 1.05E-112 | -1.69E-46 |
| MS.gene056923 | MS.gene06593 | 0.860643 | 1.79E-63 | -1.69E-46 |
| MS.gene056923 | MS.gene066937 | 0.859096 | 5.24E-63 | -1.69E-46 |
| MS.gene056923 | MS.gene06756 | 0.828566 | 8.37E-55 | -1.69E-46 |
| MS.gene056923 | MS.gene06805 | 0.860345 | 2.20E-63 | -1.69E-46 |
| MS.gene056923 | MS.gene068297 | 0.802053 | 6.73E-49 | -1.69E-46 |
| MS.gene056923 | MS.gene069482 | 0.816943 | 4.26E-52 | -1.69E-46 |
| MS.gene056923 | MS.gene071108 | 0.850474 | 1.66E-60 | -1.69E-46 |
| MS.gene056923 | MS.gene071840 | 0.82358 | 1.28E-53 | -1.69E-46 |
| MS.gene056923 | MS.gene07241 | 0.816131 | 6.48E-52 | -1.69E-46 |
| MS.gene056923 | MS.gene073526 | 0.806003 | 1.02E-49 | -1.69E-46 |
| MS.gene056923 | MS.gene073870 | 0.803786 | 2.95E-49 | -1.69E-46 |
| MS.gene056923 | MS.gene074299 | 0.817247 | 3.64E-52 | -1.69E-46 |
| MS.gene056923 | MS.gene09920 | 0.800365 | 1.49E-48 | -1.69E-46 |
| MS.gene056923 | MS.gene20763 | 0.821073 | 4.90E-53 | -1.69E-46 |
| MS.gene056923 | MS.gene23026 | 0.816787 | 4.62E-52 | -1.69E-46 |
| MS.gene056923 | MS.gene23507 | 0.820817 | 5.61E-53 | -1.69E-46 |
| MS.gene056923 | MS.gene23869 | 0.837325 | 5.56E-57 | -1.69E-46 |
| MS.gene056923 | MS.gene24108 | 0.82419 | 9.23E-54 | -1.69E-46 |
| MS.gene056923 | MS.gene24862 | 0.801253 | 9.82E-49 | -1.69E-46 |
| MS.gene056923 | MS.gene24881 | 0.818452 | 1.95E-52 | -1.69E-46 |
| MS.gene056923 | MS.gene25114 | 0.805712 | 1.17E-49 | -1.69E-46 |
| MS.gene056923 | MS.gene25138 | 0.805567 | 1.25E-49 | -1.69E-46 |
| MS.gene056923 | MS.gene25652 | 0.831756 | 1.39E-55 | -1.69E-46 |
| MS.gene056923 | MS.gene26285 | 0.813956 | 1.97E-51 | -1.69E-46 |
| MS.gene056923 | MS.gene26289 | 0.86419 | 1.45E-64 | -1.69E-46 |
| MS.gene056923 | MS.gene28548 | 0.831146 | 1.97E-55 | -1.69E-46 |
| MS.gene056923 | MS.gene30382 | 0.813151 | 2.96E-51 | -1.69E-46 |
| MS.gene056923 | MS.gene30666 | 0.854548 | 1.14E-61 | -1.69E-46 |
| MS.gene056923 | MS.gene32342 | 0.821296 | 4.35E-53 | -1.69E-46 |
| MS.gene056923 | MS.gene33335 | 0.832462 | 9.33E-56 | -1.69E-46 |
| MS.gene056923 | MS.gene33992 | 0.814375 | 1.59E-51 | -1.69E-46 |
| MS.gene056923 | MS.gene35433 | 0.849305 | 3.52E-60 | -1.69E-46 |
| MS.gene056923 | MS.gene35459 | 0.800713 | 1.27E-48 | -1.69E-46 |
| MS.gene056923 | MS.gene35460 | 0.822853 | 1.90E-53 | -1.69E-46 |
| MS.gene056923 | MS.gene35690 | 0.860088 | 2.64E-63 | -1.69E-46 |
| MS.gene056923 | MS.gene35781 | 0.810961 | 8.89E-51 | -1.69E-46 |
| MS.gene056923 | MS.gene35951 | 0.803098 | 4.10E-49 | -1.69E-46 |
| MS.gene056923 | MS.gene36173 | 0.806333 | 8.66E-50 | -1.69E-46 |
| MS.gene056923 | MS.gene37749 | 0.808705 | 2.72E-50 | -1.69E-46 |
| MS.gene056923 | MS.gene38338 | 0.819142 | 1.36E-52 | -1.69E-46 |
| MS.gene056923 | MS.gene38435 | 0.813459 | 2.53E-51 | -1.69E-46 |
| MS.gene056923 | MS.gene39756 | 0.802137 | 6.47E-49 | -1.69E-46 |
| MS.gene056923 | MS.gene40846 | 0.828644 | 8.02E-55 | -1.69E-46 |
| MS.gene056923 | MS.gene42005 | 0.816566 | 5.18E-52 | -1.69E-46 |
| MS.gene056923 | MS.gene42080 | 0.802024 | 6.83E-49 | -1.69E-46 |
| MS.gene056923 | MS.gene42096 | 0.829306 | 5.54E-55 | -1.69E-46 |
| MS.gene056923 | MS.gene43162 | 0.851712 | 7.43E-61 | -1.69E-46 |
| MS.gene056923 | MS.gene43874 | 0.81536 | 9.62E-52 | -1.69E-46 |
| MS.gene056923 | MS.gene44712 | 0.80713 | 5.87E-50 | -1.69E-46 |
| MS.gene056923 | MS.gene44958 | 0.807979 | 3.88E-50 | -1.69E-46 |
| MS.gene056923 | MS.gene45463 | 0.831973 | 1.23E-55 | -1.69E-46 |
| MS.gene056923 | MS.gene45694 | 0.834127 | 3.59E-56 | -1.69E-46 |
| MS.gene056923 | MS.gene46275 | 0.809529 | 1.81E-50 | -1.69E-46 |
| MS.gene056923 | MS.gene48169 | 0.863271 | 2.81E-64 | -1.69E-46 |
| MS.gene056923 | MS.gene49021 | 0.810175 | 1.31E-50 | -1.69E-46 |
| MS.gene056923 | MS.gene50066 | 0.802644 | 5.09E-49 | -1.69E-46 |
| MS.gene056923 | MS.gene50069 | 0.832489 | 9.18E-56 | -1.69E-46 |
| MS.gene056923 | MS.gene51331 | 0.837408 | 5.29E-57 | -1.69E-46 |
| MS.gene056923 | MS.gene54346 | 0.830165 | 3.42E-55 | -1.69E-46 |
| MS.gene056923 | MS.gene54611 | 0.853233 | 2.74E-61 | -1.69E-46 |
| MS.gene056923 | MS.gene56528 | 0.839138 | 1.90E-57 | -1.69E-46 |
| MS.gene056923 | MS.gene57807 | 0.831073 | 2.05E-55 | -1.69E-46 |
| MS.gene056923 | MS.gene58458 | 0.822288 | 2.57E-53 | -1.69E-46 |
| MS.gene056923 | MS.gene58786 | 0.80513 | 1.55E-49 | -1.69E-46 |
| MS.gene056923 | MS.gene59006 | 0.8016 | 8.34E-49 | -1.69E-46 |
| MS.gene056923 | MS.gene59920 | 0.810955 | 8.91E-51 | -1.69E-46 |
| MS.gene056923 | MS.gene60581 | 0.820903 | 5.36E-53 | -1.69E-46 |
| MS.gene056923 | MS.gene63913 | 0.805256 | 1.46E-49 | -1.69E-46 |
| MS.gene056923 | MS.gene64276 | 0.803226 | 3.86E-49 | -1.69E-46 |
| MS.gene056923 | MS.gene64584 | 0.871369 | 7.20E-67 | -1.69E-46 |
| MS.gene056923 | MS.gene65144 | 0.853001 | 3.19E-61 | -1.69E-46 |
| MS.gene056923 | MS.gene65724 | 0.804731 | 1.88E-49 | -1.69E-46 |
| MS.gene056923 | MS.gene67371 | 0.880503 | 5.20E-70 | -1.69E-46 |
| MS.gene056923 | MS.gene67377 | 0.816427 | 5.56E-52 | -1.69E-46 |
| MS.gene056923 | MS.gene67670 | 0.822996 | 1.76E-53 | -1.69E-46 |
| MS.gene056923 | MS.gene69355 | 0.810066 | 1.39E-50 | -1.69E-46 |
| MS.gene056923 | MS.gene70062 | 0.83124 | 1.87E-55 | -1.69E-46 |
| MS.gene056923 | MS.gene70064 | 0.802873 | 4.56E-49 | -1.69E-46 |
| MS.gene056923 | MS.gene71342 | 0.857735 | 1.33E-62 | -1.69E-46 |
| MS.gene056923 | MS.gene71346 | 0.851924 | 6.46E-61 | -1.69E-46 |
| MS.gene056923 | MS.gene73993 | 0.807237 | 5.58E-50 | -1.69E-46 |
| MS.gene056923 | MS.gene73995 | 0.801336 | 9.45E-49 | -1.69E-46 |
| MS.gene056923 | MS.gene74553 | 0.812679 | 3.75E-51 | -1.69E-46 |
| MS.gene056923 | MS.gene75460 | 0.84803 | 7.95E-60 | -1.69E-46 |
| MS.gene056923 | MS.gene79232 | 0.84764 | 1.02E-59 | -1.69E-46 |
| MS.gene056923 | MS.gene80293 | 0.833415 | 5.40E-56 | -1.69E-46 |
| MS.gene056923 | MS.gene80744 | 0.831551 | 1.57E-55 | -1.69E-46 |
| MS.gene056923 | MS.gene81770 | 0.91663 | 1.41E-85 | -1.69E-46 |
| MS.gene056923 | MS.gene81771 | 0.847971 | 8.25E-60 | -1.69E-46 |
| MS.gene056923 | MS.gene81774 | 0.881778 | 1.81E-70 | -1.69E-46 |
| MS.gene056923 | MS.gene82245 | 0.812216 | 4.74E-51 | -1.69E-46 |
| MS.gene056923 | MS.gene83008 | 0.806923 | 6.50E-50 | -1.69E-46 |
| MS.gene056923 | MS.gene83011 | 0.822354 | 2.48E-53 | -1.69E-46 |
| MS.gene056923 | MS.gene83366 | 0.810267 | 1.25E-50 | -1.69E-46 |
| MS.gene056923 | MS.gene84143 | 0.842297 | 2.82E-58 | -1.69E-46 |
| MS.gene056923 | MS.gene84373 | 0.826869 | 2.14E-54 | -1.69E-46 |
| MS.gene056923 | MS.gene85750 | 0.876299 | 1.56E-68 | -1.69E-46 |
| MS.gene056923 | MS.gene87043 | 0.842979 | 1.86E-58 | -1.69E-46 |
| MS.gene056923 | MS.gene87222 | 0.86028 | 2.31E-63 | -1.69E-46 |
| MS.gene056923 | MS.gene87346 | 0.811541 | 6.65E-51 | -1.69E-46 |
| MS.gene056923 | MS.gene88971 | 0.84195 | 3.48E-58 | -1.69E-46 |
| MS.gene056923 | MS.gene90814 | 0.824963 | 6.07E-54 | -1.69E-46 |
| MS.gene056923 | MS.gene91221 | 0.865646 | 5.08E-65 | -1.69E-46 |
| MS.gene056923 | MS.gene91222 | 0.85341 | 2.43E-61 | -1.69E-46 |
| MS.gene056923 | MS.gene91228 | 0.805864 | 1.09E-49 | -1.69E-46 |
| MS.gene056923 | MS.gene91754 | 0.835029 | 2.13E-56 | -1.69E-46 |
| MS.gene056923 | MS.gene93200 | 0.822522 | 2.26E-53 | -1.69E-46 |
| MS.gene056923 | MS.gene93632 | 0.821621 | 3.66E-53 | -1.69E-46 |
| MS.gene056923 | MS.gene95352 | 0.801555 | 8.52E-49 | -1.69E-46 |
| MS.gene056923 | MS.gene95353 | 0.800591 | 1.34E-48 | -1.69E-46 |
| MS.gene056923 | MS.gene96782 | 0.839092 | 1.95E-57 | -1.69E-46 |
| MS.gene056923 | MS.gene97024 | 0.828796 | 7.37E-55 | -1.69E-46 |
| MS.gene056923 | MS.gene97182 | 0.870682 | 1.21E-66 | -1.69E-46 |
| MS.gene056923 | MS.gene97293 | 0.840774 | 7.10E-58 | -1.69E-46 |
| MS.gene056923 | MS.gene97335 | 0.834054 | 3.74E-56 | -1.69E-46 |
| MS.gene056923 | MS.gene97561 | 0.807154 | 5.81E-50 | -1.69E-46 |
| MS.gene056923 | MS.gene97790 | 0.835848 | 1.32E-56 | -1.69E-46 |
| MS.gene056923 | MS.gene98466 | 0.815835 | 7.54E-52 | -1.69E-46 |
| MS.gene056923 | MS.gene98470 | 0.819914 | 9.04E-53 | -1.69E-46 |
| MS.gene056923 | MS.gene98920 | 0.801236 | 9.90E-49 | -1.69E-46 |
| MS.gene056923 | MS.gene99544 | 0.822895 | 1.85E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene81804 | MS.gene056923 | PPI |
| MS.gene05032 | MS.gene056923 | PPI |
| MS.gene017305 | MS.gene056923 | PPI |
| MS.gene056923 | MS.gene049677 | PPI |
| MS.gene05033 | MS.gene056923 | PPI |
| MS.gene26200 | MS.gene056923 | PPI |
| MS.gene008354 | MS.gene056923 | PPI |
| MS.gene061583 | MS.gene056923 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056923.t1 | MTR_1g058570 | 96.907 | 194 | 6 | 0 | 1 | 194 | 1 | 194 | 2.18e-136 | 379 |
| MS.gene056923.t1 | MTR_1g058540 | 79.082 | 196 | 39 | 1 | 1 | 194 | 1 | 196 | 1.02e-100 | 289 |
| MS.gene056923.t1 | MTR_1g058560 | 87.692 | 195 | 23 | 1 | 1 | 194 | 1 | 195 | 3.34e-100 | 288 |
| MS.gene056923.t1 | MTR_2g086740 | 56.818 | 176 | 76 | 0 | 18 | 193 | 44 | 219 | 1.98e-57 | 180 |
| MS.gene056923.t1 | MTR_7g011090 | 49.180 | 183 | 89 | 1 | 12 | 194 | 8 | 186 | 6.88e-55 | 172 |
| MS.gene056923.t1 | MTR_4g081880 | 43.784 | 185 | 99 | 2 | 9 | 193 | 7 | 186 | 7.07e-49 | 157 |
| MS.gene056923.t1 | MTR_5g041900 | 46.108 | 167 | 90 | 0 | 28 | 194 | 20 | 186 | 8.98e-43 | 142 |
| MS.gene056923.t1 | MTR_2g086740 | 57.944 | 107 | 45 | 0 | 18 | 124 | 44 | 150 | 1.84e-32 | 114 |
| MS.gene056923.t1 | MTR_0004s0210 | 35.849 | 159 | 89 | 4 | 31 | 187 | 3 | 150 | 2.16e-18 | 78.6 |
| MS.gene056923.t1 | MTR_7g108130 | 28.049 | 164 | 113 | 3 | 31 | 191 | 24 | 185 | 8.65e-14 | 67.0 |
| MS.gene056923.t1 | MTR_0097s0070 | 31.356 | 118 | 79 | 2 | 71 | 187 | 69 | 185 | 9.25e-13 | 64.3 |
| MS.gene056923.t1 | MTR_8g089300 | 31.013 | 158 | 96 | 4 | 40 | 187 | 30 | 184 | 1.10e-11 | 61.2 |
| MS.gene056923.t1 | MTR_3g064310 | 32.203 | 118 | 77 | 2 | 74 | 191 | 77 | 191 | 4.79e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056923.t1 | AT2G36100 | 58.911 | 202 | 74 | 1 | 2 | 194 | 5 | 206 | 3.22e-72 | 217 |
| MS.gene056923.t1 | AT5G06200 | 61.929 | 197 | 70 | 2 | 2 | 193 | 5 | 201 | 9.82e-65 | 198 |
| MS.gene056923.t1 | AT3G11550 | 61.000 | 200 | 71 | 2 | 2 | 194 | 5 | 204 | 3.59e-63 | 194 |
| MS.gene056923.t1 | AT2G27370 | 58.929 | 168 | 69 | 0 | 26 | 193 | 52 | 219 | 5.76e-61 | 189 |
| MS.gene056923.t1 | AT5G15290 | 48.000 | 150 | 78 | 0 | 25 | 174 | 17 | 166 | 1.12e-47 | 154 |
| MS.gene056923.t1 | AT1G14160 | 40.606 | 165 | 98 | 0 | 30 | 194 | 45 | 209 | 1.35e-41 | 139 |
| MS.gene056923.t1 | AT1G03700 | 32.215 | 149 | 92 | 2 | 39 | 187 | 12 | 151 | 7.01e-19 | 79.7 |
| MS.gene056923.t1 | AT4G03540 | 33.058 | 121 | 72 | 2 | 55 | 175 | 28 | 139 | 2.78e-15 | 70.5 |
| MS.gene056923.t1 | AT1G17200 | 27.545 | 167 | 109 | 4 | 29 | 191 | 23 | 181 | 3.14e-14 | 68.6 |
| MS.gene056923.t1 | AT4G15630 | 28.492 | 179 | 108 | 5 | 25 | 191 | 19 | 189 | 7.25e-14 | 67.4 |
| MS.gene056923.t1 | AT5G44550 | 30.263 | 152 | 97 | 3 | 40 | 183 | 22 | 172 | 2.26e-12 | 63.2 |
| MS.gene056923.t1 | AT3G06390 | 28.571 | 133 | 90 | 2 | 36 | 163 | 37 | 169 | 4.21e-12 | 62.8 |
Find 44 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGGTAATGTTGCTTGATTC+TGG | 0.093003 | 1.1:+45448751 | None:intergenic |
| CTAGCCATTTGCAACCAATT+TGG | 0.242258 | 1.1:-45448060 | MS.gene056923:CDS |
| GTGATGTCTGCTTTGGCTCT+TGG | 0.283856 | 1.1:-45447964 | MS.gene056923:CDS |
| AAGGCCTGGAGGATGGAAAA+AGG | 0.302153 | 1.1:-45448701 | MS.gene056923:CDS |
| CCTTAGAATGAAATCCATTA+TGG | 0.309686 | 1.1:+45448672 | None:intergenic |
| CCATAATGGATTTCATTCTA+AGG | 0.312115 | 1.1:-45448672 | MS.gene056923:CDS |
| TTCCATCCTCCAGGCCTTGT+AGG | 0.319831 | 1.1:+45448706 | None:intergenic |
| TTACGATATCTAAGATAATA+AGG | 0.319981 | 1.1:+45448341 | None:intergenic |
| TGATGTCTGCTTTGGCTCTT+GGG | 0.345325 | 1.1:-45447963 | MS.gene056923:CDS |
| TATTGCCGCTGCCGCCTCAA+TGG | 0.357738 | 1.1:-45448629 | MS.gene056923:CDS |
| ATTGCCGCTGCCGCCTCAAT+GGG | 0.364904 | 1.1:-45448628 | MS.gene056923:CDS |
| TGGTAATGTTGCTTGATTCT+GGG | 0.370302 | 1.1:+45448752 | None:intergenic |
| ATGGATTTCATTCTAAGGTT+AGG | 0.379588 | 1.1:-45448667 | MS.gene056923:CDS |
| AGCTGCTTCCATAGTTTACT+TGG | 0.387801 | 1.1:-45448112 | MS.gene056923:CDS |
| GTTGGTTGTGATGTCTGCTT+TGG | 0.390211 | 1.1:-45447971 | MS.gene056923:CDS |
| GTGCACAGAAATCTCCAAAT+TGG | 0.409853 | 1.1:+45448046 | None:intergenic |
| ATGGAAATCAAGACACAAAC+TGG | 0.414877 | 1.1:-45448083 | MS.gene056923:CDS |
| AGCTGCAGCAGCACTTGAAG+TGG | 0.421850 | 1.1:+45448131 | None:intergenic |
| AAGCCTTGGTCCAGGCGCAT+GGG | 0.441787 | 1.1:+45448366 | None:intergenic |
| AAAGCCTTGGTCCAGGCGCA+TGG | 0.443522 | 1.1:+45448365 | None:intergenic |
| AGCGGCAATAGCCGCTGCTA+TGG | 0.444367 | 1.1:+45448642 | None:intergenic |
| ATCTCCAAATTGGTTGCAAA+TGG | 0.445100 | 1.1:+45448056 | None:intergenic |
| TGGTAGGGATAGAACTAAGT+AGG | 0.473587 | 1.1:+45448414 | None:intergenic |
| TGTTCCCATTGAGGCGGCAG+CGG | 0.482406 | 1.1:+45448624 | None:intergenic |
| CTAGTTGCACCTACAAGGCC+TGG | 0.489392 | 1.1:-45448715 | MS.gene056923:CDS |
| ATTGTGTGCCAAGTAAACTA+TGG | 0.493367 | 1.1:+45448104 | None:intergenic |
| GCTTGATTCTGGGACATCAA+TGG | 0.503870 | 1.1:+45448762 | None:intergenic |
| TGGCTACGACAGAGAATGGT+AGG | 0.504458 | 1.1:+45448398 | None:intergenic |
| CAATTCTAGTTGCACCTACA+AGG | 0.507515 | 1.1:-45448720 | MS.gene056923:CDS |
| CACCTACAAGGCCTGGAGGA+TGG | 0.513895 | 1.1:-45448708 | MS.gene056923:CDS |
| CTGATCACTTGTTCCCATTG+AGG | 0.525536 | 1.1:+45448615 | None:intergenic |
| AGCCTTGGTCCAGGCGCATG+GGG | 0.531806 | 1.1:+45448367 | None:intergenic |
| ATGATGGCTACGACAGAGAA+TGG | 0.540767 | 1.1:+45448394 | None:intergenic |
| ATAAGGAAAAGCCTTGGTCC+AGG | 0.552077 | 1.1:+45448358 | None:intergenic |
| AAGATAATAAGGAAAAGCCT+TGG | 0.569952 | 1.1:+45448352 | None:intergenic |
| ATCACTTGTTCCCATTGAGG+CGG | 0.575884 | 1.1:+45448618 | None:intergenic |
| AGTTGCATCATTTATTACTG+TGG | 0.594258 | 1.1:-45448007 | MS.gene056923:CDS |
| ATAGTTTACTTGGCACACAA+TGG | 0.619739 | 1.1:-45448102 | MS.gene056923:CDS |
| AAGGTTAGGTGCCATAGCAG+CGG | 0.656083 | 1.1:-45448653 | MS.gene056923:CDS |
| GGCTACGACAGAGAATGGTA+GGG | 0.661247 | 1.1:+45448399 | None:intergenic |
| GCCTTGGTCCAGGCGCATGG+GGG | 0.663208 | 1.1:+45448368 | None:intergenic |
| AGAATCAAGCAACATTACCA+AGG | 0.678912 | 1.1:-45448749 | MS.gene056923:CDS |
| GATTTCTGTGCACAGACAAG+TGG | 0.683686 | 1.1:-45448036 | MS.gene056923:CDS |
| GTTGCACCTACAAGGCCTGG+AGG | 0.707990 | 1.1:-45448712 | MS.gene056923:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGTTAATAAAAATATGATAA+GGG | - | chr1.1:45448455-45448474 | MS.gene056923:intron | 10.0% |
| !! | TTGTTAATAAAAATATGATA+AGG | - | chr1.1:45448454-45448473 | MS.gene056923:intron | 10.0% |
| !! | ATTAAATTGTATACATCAAT+CGG | + | chr1.1:45448494-45448513 | None:intergenic | 15.0% |
| !! | GTTAATAAAAATATGATAAG+GGG | - | chr1.1:45448456-45448475 | MS.gene056923:intron | 15.0% |
| !!! | GTATTAGCTTATTATAATTA+TGG | + | chr1.1:45448246-45448265 | None:intergenic | 15.0% |
| !!! | AACTGTGTTTATGAAATTTT+AGG | - | chr1.1:45448557-45448576 | MS.gene056923:CDS | 20.0% |
| !!! | TTACGATATCTAAGATAATA+AGG | + | chr1.1:45448388-45448407 | None:intergenic | 20.0% |
| !!! | AACTAGAATTGTTTTTTCCT+TGG | + | chr1.1:45447997-45448016 | None:intergenic | 25.0% |
| !!! | CAGGTTTTTTGTTATTTCAA+TGG | - | chr1.1:45448277-45448296 | MS.gene056923:intron | 25.0% |
| AAGATAATAAGGAAAAGCCT+TGG | + | chr1.1:45448377-45448396 | None:intergenic | 30.0% | |
| AGTTGCATCATTTATTACTG+TGG | - | chr1.1:45448719-45448738 | MS.gene056923:CDS | 30.0% | |
| CCTTAGAATGAAATCCATTA+TGG | + | chr1.1:45448057-45448076 | None:intergenic | 30.0% | |
| GAAAGAACTGAGTGAAAAAA+GGG | + | chr1.1:45448142-45448161 | None:intergenic | 30.0% | |
| TAAAAATATGATAAGGGGTG+CGG | - | chr1.1:45448461-45448480 | MS.gene056923:intron | 30.0% | |
| TGAAAGAACTGAGTGAAAAA+AGG | + | chr1.1:45448143-45448162 | None:intergenic | 30.0% | |
| !! | ATGGATTTCATTCTAAGGTT+AGG | - | chr1.1:45448059-45448078 | MS.gene056923:CDS | 30.0% |
| !! | ATTTTAGGTGTTTCTTACTC+TGG | - | chr1.1:45448572-45448591 | MS.gene056923:CDS | 30.0% |
| !! | CCATAATGGATTTCATTCTA+AGG | - | chr1.1:45448054-45448073 | MS.gene056923:CDS | 30.0% |
| !!! | AAGTTTTGAGTGTTACATAC+TGG | + | chr1.1:45448199-45448218 | None:intergenic | 30.0% |
| !!! | TGTGGTTGTTTTAATCTTGT+TGG | - | chr1.1:45448737-45448756 | MS.gene056923:CDS | 30.0% |
| AGAATCAAGCAACATTACCA+AGG | - | chr1.1:45447977-45447996 | MS.gene056923:CDS | 35.0% | |
| ATAGTTTACTTGGCACACAA+TGG | - | chr1.1:45448624-45448643 | MS.gene056923:CDS | 35.0% | |
| ATCTCCAAATTGGTTGCAAA+TGG | + | chr1.1:45448673-45448692 | None:intergenic | 35.0% | |
| ATGGAAATCAAGACACAAAC+TGG | - | chr1.1:45448643-45448662 | MS.gene056923:CDS | 35.0% | |
| ATTGTGTGCCAAGTAAACTA+TGG | + | chr1.1:45448625-45448644 | None:intergenic | 35.0% | |
| GCTAATACTTGAAACAATGC+AGG | - | chr1.1:45448258-45448277 | MS.gene056923:intron | 35.0% | |
| TTTGAGTGTTACATACTGGA+AGG | + | chr1.1:45448195-45448214 | None:intergenic | 35.0% | |
| ! | TGGTAATGTTGCTTGATTCT+GGG | + | chr1.1:45447977-45447996 | None:intergenic | 35.0% |
| !! | TTGGTAATGTTGCTTGATTC+TGG | + | chr1.1:45447978-45447997 | None:intergenic | 35.0% |
| AGCTGCTTCCATAGTTTACT+TGG | - | chr1.1:45448614-45448633 | MS.gene056923:CDS | 40.0% | |
| CTAGCCATTTGCAACCAATT+TGG | - | chr1.1:45448666-45448685 | MS.gene056923:CDS | 40.0% | |
| GAAAAAGGGAACAGCCATAA+TGG | - | chr1.1:45448040-45448059 | MS.gene056923:CDS | 40.0% | |
| GTGCACAGAAATCTCCAAAT+TGG | + | chr1.1:45448683-45448702 | None:intergenic | 40.0% | |
| TGGTAGGGATAGAACTAAGT+AGG | + | chr1.1:45448315-45448334 | None:intergenic | 40.0% | |
| ! | CAATTCTAGTTGCACCTACA+AGG | - | chr1.1:45448006-45448025 | MS.gene056923:CDS | 40.0% |
| ATAAGGAAAAGCCTTGGTCC+AGG | + | chr1.1:45448371-45448390 | None:intergenic | 45.0% | |
| ATCACTTGTTCCCATTGAGG+CGG | + | chr1.1:45448111-45448130 | None:intergenic | 45.0% | |
| ATGATGGCTACGACAGAGAA+TGG | + | chr1.1:45448335-45448354 | None:intergenic | 45.0% | |
| CTGATCACTTGTTCCCATTG+AGG | + | chr1.1:45448114-45448133 | None:intergenic | 45.0% | |
| GATTTCTGTGCACAGACAAG+TGG | - | chr1.1:45448690-45448709 | MS.gene056923:CDS | 45.0% | |
| GTTGGTTGTGATGTCTGCTT+TGG | - | chr1.1:45448755-45448774 | MS.gene056923:CDS | 45.0% | |
| !! | GCTTGATTCTGGGACATCAA+TGG | + | chr1.1:45447967-45447986 | None:intergenic | 45.0% |
| !! | TGATGTCTGCTTTGGCTCTT+GGG | - | chr1.1:45448763-45448782 | MS.gene056923:CDS | 45.0% |
| AAGGCCTGGAGGATGGAAAA+AGG | - | chr1.1:45448025-45448044 | MS.gene056923:CDS | 50.0% | |
| AGGCCTGGAGGATGGAAAAA+GGG | - | chr1.1:45448026-45448045 | MS.gene056923:CDS | 50.0% | |
| GGCTACGACAGAGAATGGTA+GGG | + | chr1.1:45448330-45448349 | None:intergenic | 50.0% | |
| TGGCTACGACAGAGAATGGT+AGG | + | chr1.1:45448331-45448350 | None:intergenic | 50.0% | |
| ! | AAGGTTAGGTGCCATAGCAG+CGG | - | chr1.1:45448073-45448092 | MS.gene056923:CDS | 50.0% |
| ! | GTTCCCTTTTTCCATCCTCC+AGG | + | chr1.1:45448032-45448051 | None:intergenic | 50.0% |
| !! | GTGATGTCTGCTTTGGCTCT+TGG | - | chr1.1:45448762-45448781 | MS.gene056923:CDS | 50.0% |
| AGCTGCAGCAGCACTTGAAG+TGG | + | chr1.1:45448598-45448617 | None:intergenic | 55.0% | |
| CTAGTTGCACCTACAAGGCC+TGG | - | chr1.1:45448011-45448030 | MS.gene056923:CDS | 55.0% | |
| ! | TTCCATCCTCCAGGCCTTGT+AGG | + | chr1.1:45448023-45448042 | None:intergenic | 55.0% |
| AAAGCCTTGGTCCAGGCGCA+TGG | + | chr1.1:45448364-45448383 | None:intergenic | 60.0% | |
| AAGCCTTGGTCCAGGCGCAT+GGG | + | chr1.1:45448363-45448382 | None:intergenic | 60.0% | |
| AGCGGCAATAGCCGCTGCTA+TGG | + | chr1.1:45448087-45448106 | None:intergenic | 60.0% | |
| ATTGCCGCTGCCGCCTCAAT+GGG | - | chr1.1:45448098-45448117 | MS.gene056923:CDS | 60.0% | |
| CACCTACAAGGCCTGGAGGA+TGG | - | chr1.1:45448018-45448037 | MS.gene056923:CDS | 60.0% | |
| GTTGCACCTACAAGGCCTGG+AGG | - | chr1.1:45448014-45448033 | MS.gene056923:CDS | 60.0% | |
| TATTGCCGCTGCCGCCTCAA+TGG | - | chr1.1:45448097-45448116 | MS.gene056923:CDS | 60.0% | |
| TGTTCCCATTGAGGCGGCAG+CGG | + | chr1.1:45448105-45448124 | None:intergenic | 60.0% | |
| AGCCTTGGTCCAGGCGCATG+GGG | + | chr1.1:45448362-45448381 | None:intergenic | 65.0% | |
| ATCATACGCCCCCATGCGCC+TGG | - | chr1.1:45448350-45448369 | MS.gene056923:CDS | 65.0% | |
| ! | AGGCGCATGGGGGCGTATGA+TGG | + | chr1.1:45448351-45448370 | None:intergenic | 65.0% |
| GCCTTGGTCCAGGCGCATGG+GGG | + | chr1.1:45448361-45448380 | None:intergenic | 70.0% | |
| GCCCCCATGCGCCTGGACCA+AGG | - | chr1.1:45448357-45448376 | MS.gene056923:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 45447954 | 45448794 | 45447954 | ID=MS.gene056923 |
| chr1.1 | mRNA | 45447954 | 45448794 | 45447954 | ID=MS.gene056923.t1;Parent=MS.gene056923 |
| chr1.1 | exon | 45448550 | 45448794 | 45448550 | ID=MS.gene056923.t1.exon1;Parent=MS.gene056923.t1 |
| chr1.1 | CDS | 45448550 | 45448794 | 45448550 | ID=cds.MS.gene056923.t1;Parent=MS.gene056923.t1 |
| chr1.1 | exon | 45448345 | 45448468 | 45448345 | ID=MS.gene056923.t1.exon2;Parent=MS.gene056923.t1 |
| chr1.1 | CDS | 45448345 | 45448468 | 45448345 | ID=cds.MS.gene056923.t1;Parent=MS.gene056923.t1 |
| chr1.1 | exon | 45447954 | 45448169 | 45447954 | ID=MS.gene056923.t1.exon3;Parent=MS.gene056923.t1 |
| chr1.1 | CDS | 45447954 | 45448169 | 45447954 | ID=cds.MS.gene056923.t1;Parent=MS.gene056923.t1 |
| Gene Sequence |
| Protein sequence |
