Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36225.t1 | XP_003597867.1 | 96.9 | 96 | 3 | 0 | 1 | 96 | 1 | 96 | 2.40E-40 | 174.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36225.t1 | Q2HVV6 | 96.9 | 96 | 3 | 0 | 1 | 96 | 1 | 96 | 1.7e-40 | 174.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052494 | MS.gene36225 | 0.810828 | 9.49E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36225.t1 | MTR_2g103440 | 96.875 | 96 | 3 | 0 | 1 | 96 | 1 | 96 | 7.62e-64 | 188 |
| MS.gene36225.t1 | MTR_4g011030 | 56.250 | 96 | 30 | 3 | 1 | 95 | 1 | 85 | 3.25e-23 | 85.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36225.t1 | AT1G15385 | 57.778 | 45 | 16 | 1 | 51 | 95 | 31 | 72 | 4.96e-11 | 53.9 |
Find 15 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGCCTTCATCAAGAATTTC+AGG | 0.206507 | 2.1:-2399698 | MS.gene36225:CDS |
| CATCTTCTCTCTCAAGTTGA+TGG | 0.263758 | 2.1:+2399839 | None:intergenic |
| TTTCCTGAAATTCTTGATGA+AGG | 0.417879 | 2.1:+2399695 | None:intergenic |
| TAGCTTCTTATTTGTTTCAA+AGG | 0.468483 | 2.1:+2399758 | None:intergenic |
| ATTATGAGAAAATGCTTGCA+AGG | 0.509016 | 2.1:-2399638 | MS.gene36225:CDS |
| ATCAGATCTCAAGAAAATAG+AGG | 0.513230 | 2.1:-2399871 | MS.gene36225:CDS |
| CTTGATGAAGGCATCAGCAC+TGG | 0.520908 | 2.1:+2399707 | None:intergenic |
| GGAAACAACTCGTGATTCAA+AGG | 0.528679 | 2.1:-2399677 | MS.gene36225:CDS |
| TCTTGAGATCTGATGATGGT+TGG | 0.529007 | 2.1:+2399880 | None:intergenic |
| AAAGTCATATCAACATGAGA+AGG | 0.558128 | 2.1:-2399911 | None:intergenic |
| AAACAAATAAGAAGCTAGAG+AGG | 0.570340 | 2.1:-2399752 | MS.gene36225:CDS |
| TTATGAGAAAATGCTTGCAA+GGG | 0.578068 | 2.1:-2399637 | MS.gene36225:CDS |
| ATTATCATATGATCATAATG+AGG | 0.611197 | 2.1:-2399799 | MS.gene36225:CDS |
| TATGAGAAAATGCTTGCAAG+GGG | 0.640051 | 2.1:-2399636 | MS.gene36225:CDS |
| TAATGAGGTAAAGAAAGTTG+AGG | 0.652046 | 2.1:-2399784 | MS.gene36225:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTATCATATGATCATAATG+AGG | - | chr2.1:2399727-2399746 | MS.gene36225:CDS | 20.0% |
| !! | CATTTTCTCATAATTCTCAA+TGG | + | chr2.1:2399882-2399901 | None:intergenic | 25.0% |
| !! | TAGCTTCTTATTTGTTTCAA+AGG | + | chr2.1:2399771-2399790 | None:intergenic | 25.0% |
| AAACAAATAAGAAGCTAGAG+AGG | - | chr2.1:2399774-2399793 | MS.gene36225:CDS | 30.0% | |
| ATCAGATCTCAAGAAAATAG+AGG | - | chr2.1:2399655-2399674 | MS.gene36225:CDS | 30.0% | |
| ATTATGAGAAAATGCTTGCA+AGG | - | chr2.1:2399888-2399907 | MS.gene36225:CDS | 30.0% | |
| TAATGAGGTAAAGAAAGTTG+AGG | - | chr2.1:2399742-2399761 | MS.gene36225:CDS | 30.0% | |
| TTATGAGAAAATGCTTGCAA+GGG | - | chr2.1:2399889-2399908 | MS.gene36225:CDS | 30.0% | |
| TTTCCTGAAATTCTTGATGA+AGG | + | chr2.1:2399834-2399853 | None:intergenic | 30.0% | |
| ! | ATTTTCTTGAGATCTGATGA+TGG | + | chr2.1:2399653-2399672 | None:intergenic | 30.0% |
| ATGCCTTCATCAAGAATTTC+AGG | - | chr2.1:2399828-2399847 | MS.gene36225:CDS | 35.0% | |
| TATGAGAAAATGCTTGCAAG+GGG | - | chr2.1:2399890-2399909 | MS.gene36225:CDS | 35.0% | |
| GGAAACAACTCGTGATTCAA+AGG | - | chr2.1:2399849-2399868 | MS.gene36225:CDS | 40.0% | |
| !! | CATCTTCTCTCTCAAGTTGA+TGG | + | chr2.1:2399690-2399709 | None:intergenic | 40.0% |
| !! | TCTTGAGATCTGATGATGGT+TGG | + | chr2.1:2399649-2399668 | None:intergenic | 40.0% |
| !! | CTTGATGAAGGCATCAGCAC+TGG | + | chr2.1:2399822-2399841 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 2399629 | 2399919 | 2399629 | ID=MS.gene36225 |
| chr2.1 | mRNA | 2399629 | 2399919 | 2399629 | ID=MS.gene36225.t1;Parent=MS.gene36225 |
| chr2.1 | exon | 2399629 | 2399919 | 2399629 | ID=MS.gene36225.t1.exon1;Parent=MS.gene36225.t1 |
| chr2.1 | CDS | 2399629 | 2399919 | 2399629 | ID=cds.MS.gene36225.t1;Parent=MS.gene36225.t1 |
| Gene Sequence |
| Protein sequence |
