Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36304.t1 | XP_003597762.1 | 91.7 | 121 | 3 | 3 | 1 | 119 | 88 | 203 | 1.80E-45 | 191.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36304.t1 | G7II32 | 91.7 | 121 | 3 | 3 | 1 | 119 | 88 | 203 | 1.3e-45 | 191.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050807 | MS.gene36304 | 0.82458 | 7.47E-54 | -1.69E-46 |
| MS.gene050808 | MS.gene36304 | 0.806594 | 7.63E-50 | -1.69E-46 |
| MS.gene052665 | MS.gene36304 | 0.810112 | 1.36E-50 | -1.69E-46 |
| MS.gene053630 | MS.gene36304 | 0.801821 | 7.52E-49 | -1.69E-46 |
| MS.gene058168 | MS.gene36304 | 0.804575 | 2.02E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36304.t1 | MTR_2g101980 | 84.298 | 121 | 12 | 3 | 1 | 119 | 88 | 203 | 3.05e-60 | 184 |
| MS.gene36304.t1 | MTR_0147s0050 | 51.613 | 124 | 52 | 4 | 1 | 119 | 82 | 202 | 5.16e-32 | 112 |
| MS.gene36304.t1 | MTR_0147s0020 | 68.182 | 66 | 21 | 0 | 39 | 104 | 2 | 67 | 1.44e-27 | 99.0 |
| MS.gene36304.t1 | MTR_8g103530 | 50.000 | 72 | 32 | 2 | 48 | 118 | 134 | 202 | 4.39e-16 | 70.9 |
| MS.gene36304.t1 | MTR_6g086915 | 56.250 | 48 | 21 | 0 | 48 | 95 | 121 | 168 | 7.05e-14 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36304.t1 | AT1G15260 | 51.948 | 77 | 35 | 1 | 43 | 119 | 104 | 178 | 1.63e-19 | 79.3 |
| MS.gene36304.t1 | AT3G16070 | 63.830 | 47 | 17 | 0 | 48 | 94 | 104 | 150 | 4.14e-18 | 75.1 |
Find 11 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAAAGTCAAAGGGAAGATT+TGG | 0.308261 | 2.2:-1828845 | MS.gene36304:CDS |
| TCCACAATATCTAAATAAAT+AGG | 0.349490 | 2.2:+1828932 | None:intergenic |
| GAATTAGAGATGAATGATTC+AGG | 0.387057 | 2.2:-1829016 | MS.gene36304:CDS |
| GAATGAAGGTGTTAATGAAT+TGG | 0.390905 | 2.2:-1829053 | MS.gene36304:CDS |
| AGGGAAGATTTGGATCAATC+AGG | 0.432753 | 2.2:-1828835 | MS.gene36304:CDS |
| TCCTATTTATTTAGATATTG+TGG | 0.439985 | 2.2:-1828933 | MS.gene36304:CDS |
| TGAATTGGCATTGAAGATGA+AGG | 0.456133 | 2.2:-1829038 | MS.gene36304:CDS |
| CATCAAACGTTCAAAGTCAA+AGG | 0.492509 | 2.2:-1828855 | MS.gene36304:CDS |
| AAAGGGAATAAGAAGAATGA+AGG | 0.496505 | 2.2:-1829067 | MS.gene36304:CDS |
| ATCAAACGTTCAAAGTCAAA+GGG | 0.531207 | 2.2:-1828854 | MS.gene36304:CDS |
| TGAACGTTTGATGCTGACAG+AGG | 0.640696 | 2.2:+1828865 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCCACAATATCTAAATAAAT+AGG | + | chr2.2:1829066-1829085 | None:intergenic | 20.0% |
| !! | TCCTATTTATTTAGATATTG+TGG | - | chr2.2:1829062-1829081 | MS.gene36304:CDS | 20.0% |
| ! | AGAAAGAGAAAAAAGACAAA+GGG | - | chr2.2:1828911-1828930 | MS.gene36304:CDS | 25.0% |
| ! | CAAAAAAACCTTACTGAAAA+TGG | - | chr2.2:1828886-1828905 | MS.gene36304:CDS | 25.0% |
| !!! | TTTTCAGTAAGGTTTTTTTG+TGG | + | chr2.2:1828886-1828905 | None:intergenic | 25.0% |
| AAAGGGAATAAGAAGAATGA+AGG | - | chr2.2:1828928-1828947 | MS.gene36304:CDS | 30.0% | |
| ATCAAACGTTCAAAGTCAAA+GGG | - | chr2.2:1829141-1829160 | MS.gene36304:CDS | 30.0% | |
| GAATTAGAGATGAATGATTC+AGG | - | chr2.2:1828979-1828998 | MS.gene36304:CDS | 30.0% | |
| GAGAAAGAGAAAAAAGACAA+AGG | - | chr2.2:1828910-1828929 | MS.gene36304:CDS | 30.0% | |
| !! | GAATGAAGGTGTTAATGAAT+TGG | - | chr2.2:1828942-1828961 | MS.gene36304:CDS | 30.0% |
| CATCAAACGTTCAAAGTCAA+AGG | - | chr2.2:1829140-1829159 | MS.gene36304:CDS | 35.0% | |
| TCAAAGTCAAAGGGAAGATT+TGG | - | chr2.2:1829150-1829169 | MS.gene36304:CDS | 35.0% | |
| ! | CTTTCTCTCCATTTTCAGTA+AGG | + | chr2.2:1828897-1828916 | None:intergenic | 35.0% |
| ! | TGAATTGGCATTGAAGATGA+AGG | - | chr2.2:1828957-1828976 | MS.gene36304:CDS | 35.0% |
| AGGGAAGATTTGGATCAATC+AGG | - | chr2.2:1829160-1829179 | MS.gene36304:CDS | 40.0% | |
| TGAACGTTTGATGCTGACAG+AGG | + | chr2.2:1829133-1829152 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.2 | gene | 1828829 | 1829188 | 1828829 | ID=MS.gene36304 |
| chr2.2 | mRNA | 1828829 | 1829188 | 1828829 | ID=MS.gene36304.t1;Parent=MS.gene36304 |
| chr2.2 | exon | 1828829 | 1829188 | 1828829 | ID=MS.gene36304.t1.exon1;Parent=MS.gene36304.t1 |
| chr2.2 | CDS | 1828829 | 1829188 | 1828829 | ID=cds.MS.gene36304.t1;Parent=MS.gene36304.t1 |
| Gene Sequence |
| Protein sequence |
