Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36448.t1 | XP_013457307.1 | 79 | 386 | 37 | 9 | 1 | 385 | 1 | 343 | 1.90E-144 | 522.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36448.t1 | A0A072UQL8 | 79.0 | 386 | 37 | 9 | 1 | 385 | 1 | 343 | 1.4e-144 | 522.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051837 | MS.gene36448 | 0.803939 | 2.74E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36448.t1 | MTR_4g094532 | 78.238 | 386 | 40 | 8 | 1 | 385 | 1 | 343 | 0.0 | 545 |
| MS.gene36448.t1 | MTR_2g031350 | 33.092 | 414 | 216 | 11 | 1 | 374 | 1 | 393 | 1.56e-27 | 112 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36448.t1 | AT4G20880 | 32.432 | 185 | 98 | 7 | 1 | 171 | 1 | 172 | 6.10e-13 | 70.1 |
Find 64 sgRNAs with CRISPR-Local
Find 86 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAATATTCCATCAAGAATT+TGG | 0.136566 | 4.1:-18776549 | None:intergenic |
| TTGAAACGGTTTATTGGATT+TGG | 0.215358 | 4.1:-18775563 | None:intergenic |
| ATCTATGTTGAAGAAACTTT+TGG | 0.224829 | 4.1:+18776152 | MS.gene36448:CDS |
| CTAGTTACTCTCATGTCTTC+TGG | 0.238316 | 4.1:-18776345 | None:intergenic |
| CTTGTGTTCCAAATTCTTGA+TGG | 0.265046 | 4.1:+18776541 | MS.gene36448:CDS |
| TTGAGTTTGAAACGGTTTAT+TGG | 0.270531 | 4.1:-18775569 | None:intergenic |
| AGATTTGATCAGAGGCTTCT+TGG | 0.313470 | 4.1:-18776520 | None:intergenic |
| ATTGAAGATCGGCGACGATT+TGG | 0.314295 | 4.1:-18775447 | None:intergenic |
| AGTCAACAATGCCTCTTCTA+TGG | 0.324846 | 4.1:+18775395 | None:intergenic |
| TGTCAAGGAAGATAAGAAAT+TGG | 0.333166 | 4.1:+18776050 | MS.gene36448:CDS |
| TTATTTGGAATTGCACTTGT+TGG | 0.349662 | 4.1:+18776414 | MS.gene36448:CDS |
| TGAAACGGTTTATTGGATTT+GGG | 0.352827 | 4.1:-18775562 | None:intergenic |
| TAGAGCGAAAGAGGAAATTA+AGG | 0.353220 | 4.1:+18775933 | MS.gene36448:CDS |
| TATTTGGAATTGCACTTGTT+GGG | 0.361747 | 4.1:+18776415 | MS.gene36448:CDS |
| TTCGTGTGATGGTGTTAAAC+TGG | 0.379127 | 4.1:+18776023 | MS.gene36448:CDS |
| AAATGATTCCTTGTCATTTG+AGG | 0.398194 | 4.1:+18775963 | MS.gene36448:CDS |
| TTCAGATCATGGGGTTTGTC+CGG | 0.403912 | 4.1:+18775735 | MS.gene36448:CDS |
| GAGAGTAGAAGCGGTGAAGA+TGG | 0.407608 | 4.1:+18776228 | MS.gene36448:CDS |
| TCAACGTTCTTCAAGAAAAG+AGG | 0.417426 | 4.1:+18776185 | MS.gene36448:CDS |
| TACAATGACATGGTGTTTGA+TGG | 0.419564 | 4.1:+18776470 | MS.gene36448:CDS |
| CAAGGTTGATTGCGCCGAAC+CGG | 0.430414 | 4.1:+18775636 | MS.gene36448:CDS |
| TTCTGAGAGTCGGTGTTGTC+CGG | 0.435081 | 4.1:-18775655 | None:intergenic |
| TTTGAGGAAATTGAGGTTGT+TGG | 0.437196 | 4.1:+18775979 | MS.gene36448:CDS |
| TGCACTTGTTGGGCTTGTGA+TGG | 0.437616 | 4.1:+18776425 | MS.gene36448:CDS |
| TCTTCTTTCGCCGTTCAGAC+TGG | 0.446664 | 4.1:+18775484 | MS.gene36448:CDS |
| CCGGTTGGTAAAAGTAATGA+TGG | 0.462725 | 4.1:+18775754 | MS.gene36448:CDS |
| GATCATGGGGTTTGTCCGGT+TGG | 0.463209 | 4.1:+18775739 | MS.gene36448:CDS |
| TGATGGGTCGGGTTCAAAAT+CGG | 0.463843 | 4.1:+18775771 | MS.gene36448:CDS |
| ATTAAAAGAGGAGGTGGTAG+TGG | 0.467017 | 4.1:+18776281 | MS.gene36448:CDS |
| CTTGTCATTTGAGGAAATTG+AGG | 0.476954 | 4.1:+18775972 | MS.gene36448:CDS |
| AAAAGATCGACGAGGGAGGT+TGG | 0.477129 | 4.1:-18775511 | None:intergenic |
| AAAGATCGACGAGGGAGGTT+GGG | 0.481141 | 4.1:-18775510 | None:intergenic |
| CCACATCATCGTTTACATGA+AGG | 0.486327 | 4.1:-18775705 | None:intergenic |
| CGGCGACGATTTGGAAAACT+CGG | 0.494695 | 4.1:-18775438 | None:intergenic |
| TGAAGACAGTAGATAGTGTT+GGG | 0.499506 | 4.1:+18776373 | MS.gene36448:CDS |
| GTGAAGACAGTAGATAGTGT+TGG | 0.499650 | 4.1:+18776372 | MS.gene36448:CDS |
| GTCATTGTAAGTATTAGTGC+TGG | 0.509075 | 4.1:-18776456 | None:intergenic |
| TTCGTGTTCTAGAGCGAAAG+AGG | 0.510125 | 4.1:+18775924 | MS.gene36448:CDS |
| AGAGTCGGTGTTGTCCGGTT+CGG | 0.510297 | 4.1:-18775650 | None:intergenic |
| GCACTTGTTGGGCTTGTGAT+GGG | 0.528076 | 4.1:+18776426 | MS.gene36448:CDS |
| TCAGAGGTTTGAGTTTGAAA+CGG | 0.529732 | 4.1:-18775577 | None:intergenic |
| TGGTAAAAGTAATGATGGGT+CGG | 0.542655 | 4.1:+18775759 | MS.gene36448:CDS |
| GAAGACACTTCTTCGTGTGA+TGG | 0.545282 | 4.1:+18776012 | MS.gene36448:CDS |
| GGTTTAACATTGAGCAACAA+TGG | 0.545879 | 4.1:-18776324 | None:intergenic |
| GGTAAAAGTAATGATGGGTC+GGG | 0.548543 | 4.1:+18775760 | MS.gene36448:CDS |
| CTCAATTTCCTCAAATGACA+AGG | 0.552693 | 4.1:-18775971 | None:intergenic |
| GAGGTGGTAGTGGTAGATAG+TGG | 0.556356 | 4.1:+18776291 | MS.gene36448:CDS |
| TCGGGTTCAAAATCGGTACT+TGG | 0.556494 | 4.1:+18775778 | MS.gene36448:CDS |
| CTCTGACGGTGGTGGATCAG+AGG | 0.559702 | 4.1:-18775593 | None:intergenic |
| TGCAAAGTTGAGAGTAGAAG+CGG | 0.561798 | 4.1:+18776219 | MS.gene36448:CDS |
| GGAACACAAGATTTGATCAG+AGG | 0.566594 | 4.1:-18776528 | None:intergenic |
| ATGGGTGTCTCTGACGGTGG+TGG | 0.567189 | 4.1:-18775601 | None:intergenic |
| CCTTCATGTAAACGATGATG+TGG | 0.574752 | 4.1:+18775705 | MS.gene36448:CDS |
| CACTAATACTTACAATGACA+TGG | 0.584897 | 4.1:+18776460 | MS.gene36448:CDS |
| GCTTCGTCGTCATGTATTCG+AGG | 0.587029 | 4.1:-18775894 | None:intergenic |
| AAAATGGGTGTCTCTGACGG+TGG | 0.599069 | 4.1:-18775604 | None:intergenic |
| GCGTTTAGATGATTGAAGAT+CGG | 0.601462 | 4.1:-18775458 | None:intergenic |
| CTGAGTTAAATTCTGAGAGT+CGG | 0.604404 | 4.1:-18775665 | None:intergenic |
| GGTTGGGAAACCAGTCTGAA+CGG | 0.607212 | 4.1:-18775494 | None:intergenic |
| CGGTTGGTAAAAGTAATGAT+GGG | 0.627482 | 4.1:+18775755 | MS.gene36448:CDS |
| ATACATGACGACGAAGCATG+TGG | 0.656709 | 4.1:+18775900 | MS.gene36448:CDS |
| TATTAAAAGATCGACGAGGG+AGG | 0.663174 | 4.1:-18775515 | None:intergenic |
| TGTTAAACTGGATTGTGTCA+AGG | 0.671675 | 4.1:+18776035 | MS.gene36448:CDS |
| GTAAAGATAGCTGCAGTACG+AGG | 0.678373 | 4.1:+18776492 | MS.gene36448:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAAGATTTTGAATTAAAAG+AGG | + | chr4.1:18776269-18776288 | MS.gene36448:CDS | 15.0% |
| !!! | TCAGATTACATGATTTTATT+TGG | + | chr4.1:18776399-18776418 | MS.gene36448:CDS | 20.0% |
| !! | AGATTTTGAATTAAAAGAGG+AGG | + | chr4.1:18776272-18776291 | MS.gene36448:CDS | 25.0% |
| !! | ATCTATGTTGAAGAAACTTT+TGG | + | chr4.1:18776152-18776171 | MS.gene36448:CDS | 25.0% |
| !! | TCTATGTTGAAGAAACTTTT+GGG | + | chr4.1:18776153-18776172 | MS.gene36448:CDS | 25.0% |
| !! | TTTTATTAAAAGATCGACGA+GGG | - | chr4.1:18775521-18775540 | None:intergenic | 25.0% |
| !! | TTTTTATTAAAAGATCGACG+AGG | - | chr4.1:18775522-18775541 | None:intergenic | 25.0% |
| AACCTTGCAATCGTTAAAAA+TGG | - | chr4.1:18775623-18775642 | None:intergenic | 30.0% | |
| ACCTTGCAATCGTTAAAAAT+GGG | - | chr4.1:18775622-18775641 | None:intergenic | 30.0% | |
| CACTAATACTTACAATGACA+TGG | + | chr4.1:18776460-18776479 | MS.gene36448:CDS | 30.0% | |
| TGAAACGGTTTATTGGATTT+GGG | - | chr4.1:18775565-18775584 | None:intergenic | 30.0% | |
| TGTCAAGGAAGATAAGAAAT+TGG | + | chr4.1:18776050-18776069 | MS.gene36448:CDS | 30.0% | |
| TTGAAACGGTTTATTGGATT+TGG | - | chr4.1:18775566-18775585 | None:intergenic | 30.0% | |
| ! | AAATGATTCCTTGTCATTTG+AGG | + | chr4.1:18775963-18775982 | MS.gene36448:CDS | 30.0% |
| ! | TATTTGGAATTGCACTTGTT+GGG | + | chr4.1:18776415-18776434 | MS.gene36448:CDS | 30.0% |
| ! | TTATTTGGAATTGCACTTGT+TGG | + | chr4.1:18776414-18776433 | MS.gene36448:CDS | 30.0% |
| ! | TTGAGTTTGAAACGGTTTAT+TGG | - | chr4.1:18775572-18775591 | None:intergenic | 30.0% |
| ! | TTTTGAATTAAAAGAGGAGG+TGG | + | chr4.1:18776275-18776294 | MS.gene36448:CDS | 30.0% |
| !!! | TGGTTTTGAATTCAGATCAT+GGG | + | chr4.1:18775725-18775744 | MS.gene36448:CDS | 30.0% |
| CTCAATTTCCTCAAATGACA+AGG | - | chr4.1:18775974-18775993 | None:intergenic | 35.0% | |
| GCGTTTAGATGATTGAAGAT+CGG | - | chr4.1:18775461-18775480 | None:intergenic | 35.0% | |
| GGTTTAACATTGAGCAACAA+TGG | - | chr4.1:18776327-18776346 | None:intergenic | 35.0% | |
| GTCATTGTAAGTATTAGTGC+TGG | - | chr4.1:18776459-18776478 | None:intergenic | 35.0% | |
| TACAATGACATGGTGTTTGA+TGG | + | chr4.1:18776470-18776489 | MS.gene36448:CDS | 35.0% | |
| TAGAGCGAAAGAGGAAATTA+AGG | + | chr4.1:18775933-18775952 | MS.gene36448:CDS | 35.0% | |
| TCAACGTTCTTCAAGAAAAG+AGG | + | chr4.1:18776185-18776204 | MS.gene36448:CDS | 35.0% | |
| TCTATGGACCAACAAAAACA+GGG | + | chr4.1:18775411-18775430 | MS.gene36448:CDS | 35.0% | |
| TGAAGACAGTAGATAGTGTT+GGG | + | chr4.1:18776373-18776392 | MS.gene36448:CDS | 35.0% | |
| TGTTAAACTGGATTGTGTCA+AGG | + | chr4.1:18776035-18776054 | MS.gene36448:CDS | 35.0% | |
| TTAAAAATGGGTGTCTCTGA+CGG | - | chr4.1:18775610-18775629 | None:intergenic | 35.0% | |
| TTCTATGGACCAACAAAAAC+AGG | + | chr4.1:18775410-18775429 | MS.gene36448:CDS | 35.0% | |
| TTTGAGGAAATTGAGGTTGT+TGG | + | chr4.1:18775979-18775998 | MS.gene36448:CDS | 35.0% | |
| ! | CTGAGTTAAATTCTGAGAGT+CGG | - | chr4.1:18775668-18775687 | None:intergenic | 35.0% |
| ! | CTTGTCATTTGAGGAAATTG+AGG | + | chr4.1:18775972-18775991 | MS.gene36448:CDS | 35.0% |
| ! | TCAGAGGTTTGAGTTTGAAA+CGG | - | chr4.1:18775580-18775599 | None:intergenic | 35.0% |
| ! | TTTTTGTTGGTCCATAGAAG+AGG | - | chr4.1:18775409-18775428 | None:intergenic | 35.0% |
| !! | ACCCATTTTTAACGATTGCA+AGG | + | chr4.1:18775618-18775637 | MS.gene36448:CDS | 35.0% |
| !! | CCATCATTACTTTTACCAAC+CGG | - | chr4.1:18775757-18775776 | None:intergenic | 35.0% |
| !! | CGGTTGGTAAAAGTAATGAT+GGG | + | chr4.1:18775755-18775774 | MS.gene36448:CDS | 35.0% |
| !! | GGAATGTTCTGAAAAAGTTG+TGG | + | chr4.1:18776071-18776090 | MS.gene36448:CDS | 35.0% |
| !! | TGGATTTGGGTTTTGTGAAA+CGG | - | chr4.1:18775552-18775571 | None:intergenic | 35.0% |
| !! | TGGTAAAAGTAATGATGGGT+CGG | + | chr4.1:18775759-18775778 | MS.gene36448:CDS | 35.0% |
| !!! | AGTTTTGAGAAACTCACTGT+TGG | + | chr4.1:18775841-18775860 | MS.gene36448:CDS | 35.0% |
| !!! | GGTTTTGAATTCAGATCATG+GGG | + | chr4.1:18775726-18775745 | MS.gene36448:CDS | 35.0% |
| !!! | GTGGTTTTGAATTCAGATCA+TGG | + | chr4.1:18775724-18775743 | MS.gene36448:CDS | 35.0% |
| !!! | GTTTTGAGAAACTCACTGTT+GGG | + | chr4.1:18775842-18775861 | MS.gene36448:CDS | 35.0% |
| AGATTTGATCAGAGGCTTCT+TGG | - | chr4.1:18776523-18776542 | None:intergenic | 40.0% | |
| ATTAAAAGAGGAGGTGGTAG+TGG | + | chr4.1:18776281-18776300 | MS.gene36448:CDS | 40.0% | |
| CCACATCATCGTTTACATGA+AGG | - | chr4.1:18775708-18775727 | None:intergenic | 40.0% | |
| CCTTCATGTAAACGATGATG+TGG | + | chr4.1:18775705-18775724 | MS.gene36448:CDS | 40.0% | |
| CTAGTTACTCTCATGTCTTC+TGG | - | chr4.1:18776348-18776367 | None:intergenic | 40.0% | |
| GGAACACAAGATTTGATCAG+AGG | - | chr4.1:18776531-18776550 | None:intergenic | 40.0% | |
| GTGAAGACAGTAGATAGTGT+TGG | + | chr4.1:18776372-18776391 | MS.gene36448:CDS | 40.0% | |
| TATTAAAAGATCGACGAGGG+AGG | - | chr4.1:18775518-18775537 | None:intergenic | 40.0% | |
| TGCAAAGTTGAGAGTAGAAG+CGG | + | chr4.1:18776219-18776238 | MS.gene36448:CDS | 40.0% | |
| ! | TTCGTGTGATGGTGTTAAAC+TGG | + | chr4.1:18776023-18776042 | MS.gene36448:CDS | 40.0% |
| !! | CCGGTTGGTAAAAGTAATGA+TGG | + | chr4.1:18775754-18775773 | MS.gene36448:CDS | 40.0% |
| !! | GGATTTGGGTTTTGTGAAAC+GGG | - | chr4.1:18775551-18775570 | None:intergenic | 40.0% |
| !! | GGTAAAAGTAATGATGGGTC+GGG | + | chr4.1:18775760-18775779 | MS.gene36448:CDS | 40.0% |
| !!! | TCGGTTTACCCTGTTTTTGT+TGG | - | chr4.1:18775422-18775441 | None:intergenic | 40.0% |
| ATACATGACGACGAAGCATG+TGG | + | chr4.1:18775900-18775919 | MS.gene36448:CDS | 45.0% | |
| ATTGAAGATCGGCGACGATT+TGG | - | chr4.1:18775450-18775469 | None:intergenic | 45.0% | |
| GAAGACACTTCTTCGTGTGA+TGG | + | chr4.1:18776012-18776031 | MS.gene36448:CDS | 45.0% | |
| GTAAAGATAGCTGCAGTACG+AGG | + | chr4.1:18776492-18776511 | MS.gene36448:CDS | 45.0% | |
| TCGGGTTCAAAATCGGTACT+TGG | + | chr4.1:18775778-18775797 | MS.gene36448:CDS | 45.0% | |
| TTCAGATCATGGGGTTTGTC+CGG | + | chr4.1:18775735-18775754 | MS.gene36448:CDS | 45.0% | |
| ! | TGATGGGTCGGGTTCAAAAT+CGG | + | chr4.1:18775771-18775790 | MS.gene36448:CDS | 45.0% |
| !! | TGGGTTTTGTGAAACGGGTA+TGG | - | chr4.1:18775546-18775565 | None:intergenic | 45.0% |
| !! | TTCGTGTTCTAGAGCGAAAG+AGG | + | chr4.1:18775924-18775943 | MS.gene36448:CDS | 45.0% |
| AAAAGATCGACGAGGGAGGT+TGG | - | chr4.1:18775514-18775533 | None:intergenic | 50.0% | |
| AAAATGGGTGTCTCTGACGG+TGG | - | chr4.1:18775607-18775626 | None:intergenic | 50.0% | |
| AAAGATCGACGAGGGAGGTT+GGG | - | chr4.1:18775513-18775532 | None:intergenic | 50.0% | |
| GAGAGTAGAAGCGGTGAAGA+TGG | + | chr4.1:18776228-18776247 | MS.gene36448:CDS | 50.0% | |
| GCTTCGTCGTCATGTATTCG+AGG | - | chr4.1:18775897-18775916 | None:intergenic | 50.0% | |
| GGTTGGGAAACCAGTCTGAA+CGG | - | chr4.1:18775497-18775516 | None:intergenic | 50.0% | |
| TCTTCTTTCGCCGTTCAGAC+TGG | + | chr4.1:18775484-18775503 | MS.gene36448:CDS | 50.0% | |
| ! | CGGCGACGATTTGGAAAACT+CGG | - | chr4.1:18775441-18775460 | None:intergenic | 50.0% |
| ! | GCACTTGTTGGGCTTGTGAT+GGG | + | chr4.1:18776426-18776445 | MS.gene36448:CDS | 50.0% |
| ! | TGCACTTGTTGGGCTTGTGA+TGG | + | chr4.1:18776425-18776444 | MS.gene36448:CDS | 50.0% |
| !! | GAGGTGGTAGTGGTAGATAG+TGG | + | chr4.1:18776291-18776310 | MS.gene36448:CDS | 50.0% |
| !! | TTCTGAGAGTCGGTGTTGTC+CGG | - | chr4.1:18775658-18775677 | None:intergenic | 50.0% |
| GATCATGGGGTTTGTCCGGT+TGG | + | chr4.1:18775739-18775758 | MS.gene36448:CDS | 55.0% | |
| ! | CAAGGTTGATTGCGCCGAAC+CGG | + | chr4.1:18775636-18775655 | MS.gene36448:CDS | 55.0% |
| !! | AGAGTCGGTGTTGTCCGGTT+CGG | - | chr4.1:18775653-18775672 | None:intergenic | 55.0% |
| ATGGGTGTCTCTGACGGTGG+TGG | - | chr4.1:18775604-18775623 | None:intergenic | 60.0% | |
| !! | CTCTGACGGTGGTGGATCAG+AGG | - | chr4.1:18775596-18775615 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 18775403 | 18776560 | 18775403 | ID=MS.gene36448 |
| chr4.1 | mRNA | 18775403 | 18776560 | 18775403 | ID=MS.gene36448.t1;Parent=MS.gene36448 |
| chr4.1 | exon | 18775403 | 18776560 | 18775403 | ID=MS.gene36448.t1.exon1;Parent=MS.gene36448.t1 |
| chr4.1 | CDS | 18775403 | 18776560 | 18775403 | ID=cds.MS.gene36448.t1;Parent=MS.gene36448.t1 |
| Gene Sequence |
| Protein sequence |
