AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene36521

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36521.t1 MTR_4g094715 96.178 471 8 1 1 471 1 461 0.0 931
MS.gene36521.t1 MTR_2g032710 74.735 471 111 3 1 471 1 463 0.0 707
MS.gene36521.t1 MTR_1g086390 63.333 390 141 1 74 461 80 469 0.0 542
MS.gene36521.t1 MTR_7g110050 60.621 419 161 3 53 469 46 462 0.0 539
MS.gene36521.t1 MTR_5g034090 43.552 473 226 7 21 458 56 522 5.13e-126 377
MS.gene36521.t1 MTR_8g065010 46.604 427 194 7 58 458 91 509 3.49e-125 374
MS.gene36521.t1 MTR_6g005630 46.348 397 195 6 75 470 45 424 6.81e-122 362
MS.gene36521.t1 MTR_6g005630 43.577 397 183 8 75 470 45 401 8.57e-108 325
MS.gene36521.t1 MTR_5g033680 40.250 400 226 6 60 456 50 439 2.19e-104 319
MS.gene36521.t1 MTR_8g065030 41.117 394 219 6 74 465 64 446 4.40e-102 313
MS.gene36521.t1 MTR_1g052315 44.101 356 185 7 70 422 59 403 3.95e-94 292
MS.gene36521.t1 MTR_7g091850 44.413 358 186 6 74 428 75 422 1.35e-92 288
MS.gene36521.t1 MTR_2g103410 40.000 380 215 7 74 450 58 427 2.36e-92 288
MS.gene36521.t1 MTR_2g060350 43.251 363 191 7 69 427 68 419 6.08e-90 281
MS.gene36521.t1 MTR_7g091840 43.769 329 171 5 69 392 59 378 2.31e-86 270
MS.gene36521.t1 MTR_5g034090 41.926 353 167 5 21 339 56 404 1.61e-81 258
MS.gene36521.t1 MTR_2g103650 36.031 383 233 7 75 453 28 402 1.41e-79 253
MS.gene36521.t1 MTR_3g030540 37.963 432 236 9 7 432 10 415 1.98e-77 248
MS.gene36521.t1 MTR_3g054320 37.245 392 220 10 75 461 30 400 4.89e-76 244
MS.gene36521.t1 MTR_6g028080 36.719 384 222 9 75 453 30 397 3.16e-74 239
MS.gene36521.t1 MTR_3g084380 35.714 406 233 10 53 453 5 387 6.43e-73 236
MS.gene36521.t1 MTR_2g049400 37.371 388 224 9 68 453 21 391 1.24e-72 234
MS.gene36521.t1 MTR_3g438050 36.883 385 223 8 74 453 26 395 1.44e-72 234
MS.gene36521.t1 MTR_1g012390 36.104 385 227 9 75 455 28 397 2.13e-70 229
MS.gene36521.t1 MTR_8g010070 35.385 390 223 10 70 455 26 390 1.26e-69 227
MS.gene36521.t1 MTR_4g084050 35.342 365 209 8 75 428 23 371 6.10e-69 225
MS.gene36521.t1 MTR_5g043350 36.020 397 226 12 66 455 23 398 1.33e-68 224
MS.gene36521.t1 MTR_4g083660 37.275 389 223 13 69 453 3 374 4.56e-68 222
MS.gene36521.t1 MTR_8g010080 35.659 387 220 10 70 453 26 386 5.86e-67 220
MS.gene36521.t1 MTR_1g088160 36.387 382 215 12 68 438 28 392 1.13e-66 219
MS.gene36521.t1 MTR_8g030930 35.754 358 210 7 74 424 26 370 1.90e-66 219
MS.gene36521.t1 MTR_8g010040 35.433 381 218 9 75 452 32 387 6.06e-66 217
MS.gene36521.t1 MTR_0157s0060 35.864 382 217 8 80 455 3 362 6.72e-66 216
MS.gene36521.t1 MTR_4g034020 35.864 382 217 8 80 455 3 362 6.72e-66 216
MS.gene36521.t1 MTR_8g008765 35.671 328 186 5 70 394 26 331 4.10e-65 214
MS.gene36521.t1 MTR_3g084360 35.278 360 211 9 75 428 24 367 6.43e-65 214
MS.gene36521.t1 MTR_2g058640 35.475 358 218 8 74 428 24 371 1.17e-64 214
MS.gene36521.t1 MTR_2g058670 34.943 352 217 7 74 422 24 366 3.77e-64 213
MS.gene36521.t1 MTR_8g010110 33.592 387 229 8 70 453 26 387 8.71e-64 211
MS.gene36521.t1 MTR_2g058840 33.983 359 209 8 74 422 24 364 1.27e-62 209
MS.gene36521.t1 MTR_8g010090 32.817 387 232 9 70 453 26 387 1.80e-62 208
MS.gene36521.t1 MTR_7g113910 35.501 369 151 8 74 440 56 339 5.32e-62 206
MS.gene36521.t1 MTR_7g035415 36.508 315 176 7 74 383 39 334 6.61e-62 205
MS.gene36521.t1 MTR_2g435640 34.181 354 217 7 74 422 52 394 6.77e-62 207
MS.gene36521.t1 MTR_8g009810 35.509 383 226 11 75 453 35 400 7.50e-62 207
MS.gene36521.t1 MTR_2g103410 38.806 268 154 4 74 339 58 317 4.58e-61 202
MS.gene36521.t1 MTR_2g029170 39.506 324 175 10 107 422 23 333 5.96e-60 201
MS.gene36521.t1 MTR_5g097860 36.036 333 182 8 74 394 24 337 7.92e-60 200
MS.gene36521.t1 MTR_1g013730 31.937 382 229 9 75 453 27 380 2.74e-57 194
MS.gene36521.t1 MTR_4g120320 33.249 394 242 9 67 450 38 420 4.12e-57 195
MS.gene36521.t1 MTR_1g013740 31.510 384 232 9 75 455 35 390 1.29e-56 192
MS.gene36521.t1 MTR_8g010050 32.216 388 200 11 70 453 26 354 1.94e-56 191
MS.gene36521.t1 MTR_8g077320 30.711 394 196 10 74 452 32 363 1.43e-51 179
MS.gene36521.t1 MTR_5g063430 32.667 300 181 6 74 369 24 306 5.74e-48 167
MS.gene36521.t1 MTR_8g008700 32.817 323 170 8 133 453 13 290 9.29e-48 166
MS.gene36521.t1 MTR_2g100040 30.447 358 183 11 70 421 20 317 7.38e-41 150
MS.gene36521.t1 MTR_8g031180 37.019 208 121 3 222 423 70 273 8.18e-39 142
MS.gene36521.t1 MTR_4g124980 27.273 407 224 15 57 422 61 436 1.81e-24 106
MS.gene36521.t1 MTR_2g042740 27.415 383 227 14 73 422 54 418 1.43e-23 103
MS.gene36521.t1 MTR_1g100040 26.074 349 211 10 77 391 58 393 9.65e-19 89.0
MS.gene36521.t1 MTR_5g090160 27.532 316 188 9 77 364 46 348 2.26e-18 87.8
MS.gene36521.t1 MTR_1g086030 27.726 321 188 9 77 368 84 389 2.42e-17 84.7
MS.gene36521.t1 MTR_7g110710 27.245 323 188 10 77 368 45 351 2.84e-17 84.3
MS.gene36521.t1 MTR_3g449590 26.074 326 200 10 66 364 39 350 3.18e-17 84.3
MS.gene36521.t1 MTR_7g110710 27.329 322 189 9 77 368 45 351 3.31e-17 84.3
MS.gene36521.t1 MTR_1g100050 25.926 351 209 14 77 391 52 387 5.15e-17 83.6
MS.gene36521.t1 MTR_7g088550 26.282 312 185 10 77 358 52 348 5.40e-17 83.6
MS.gene36521.t1 MTR_3g096330 25.526 380 227 13 9 358 21 374 6.70e-17 84.0
MS.gene36521.t1 MTR_8g098840 26.498 317 178 12 77 358 46 342 7.91e-17 83.2
MS.gene36521.t1 MTR_1g100060 24.928 349 215 10 77 391 95 430 8.05e-17 83.2
MS.gene36521.t1 MTR_1g099850 25.000 352 211 12 77 391 57 392 1.68e-16 82.0
MS.gene36521.t1 MTR_8g028725 24.633 341 215 10 80 391 60 387 3.11e-15 78.2
MS.gene36521.t1 MTR_2g435340 27.492 331 185 13 65 362 31 339 7.32e-15 77.0
MS.gene36521.t1 MTR_1g086030 28.159 277 157 8 121 368 22 285 1.94e-14 75.5
MS.gene36521.t1 MTR_4g120730 25.234 321 195 9 77 367 42 347 2.01e-14 75.5
MS.gene36521.t1 MTR_7g078680 25.484 310 190 9 77 358 57 353 6.76e-14 73.9
MS.gene36521.t1 MTR_2g435340 29.353 201 120 4 173 362 85 274 6.57e-12 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36521.t1 AT1G48100 68.898 463 139 3 1 458 1 463 0.0 657
MS.gene36521.t1 AT1G02460 61.381 391 146 3 74 459 88 478 5.26e-180 513
MS.gene36521.t1 AT1G56710 57.213 409 169 4 54 459 28 433 6.43e-170 485
MS.gene36521.t1 AT4G01890 55.583 403 173 3 63 460 53 454 8.25e-167 478
MS.gene36521.t1 AT5G14650 47.179 390 190 6 75 461 51 427 1.06e-120 360
MS.gene36521.t1 AT1G60590 43.614 415 201 6 75 462 123 531 7.14e-118 356
MS.gene36521.t1 AT1G10640 44.743 409 196 6 74 458 118 520 3.19e-116 352
MS.gene36521.t1 AT3G26610 38.925 465 256 10 1 457 7 451 4.21e-107 326
MS.gene36521.t1 AT1G80170 42.932 382 206 6 71 449 49 421 4.82e-107 325
MS.gene36521.t1 AT1G70500 41.667 396 218 6 74 467 66 450 2.23e-106 324
MS.gene36521.t1 AT1G23460 41.294 402 219 7 62 459 30 418 1.12e-100 308
MS.gene36521.t1 AT1G23460 41.294 402 219 7 62 459 54 442 1.74e-100 309
MS.gene36521.t1 AT3G57510 45.299 351 175 7 77 422 70 408 6.47e-94 291
MS.gene36521.t1 AT3G07970 39.173 411 232 9 35 441 33 429 9.47e-91 283
MS.gene36521.t1 AT2G41850 42.857 350 185 6 77 422 70 408 2.01e-88 277
MS.gene36521.t1 AT1G05660 37.887 388 223 8 70 455 23 394 6.99e-78 248
MS.gene36521.t1 AT1G05650 37.179 390 227 8 68 455 21 394 9.98e-76 243
MS.gene36521.t1 AT3G15720 38.718 390 206 12 75 454 23 389 2.96e-75 243
MS.gene36521.t1 AT3G15720 38.718 390 206 12 75 454 24 390 3.01e-75 243
MS.gene36521.t1 AT2G43890 36.979 384 224 11 67 448 21 388 1.14e-74 240
MS.gene36521.t1 AT4G35670 35.955 356 210 6 75 427 24 364 1.15e-74 240
MS.gene36521.t1 AT5G14650 44.737 266 135 3 75 339 51 305 6.28e-74 235
MS.gene36521.t1 AT2G43870 39.316 351 196 7 75 422 21 357 2.05e-73 236
MS.gene36521.t1 AT3G15720 38.974 390 202 13 75 454 23 386 7.44e-73 237
MS.gene36521.t1 AT2G43880 37.076 383 218 10 75 453 31 394 4.39e-72 233
MS.gene36521.t1 AT1G80170 42.804 271 145 4 71 339 49 311 9.16e-72 230
MS.gene36521.t1 AT5G44840 34.492 374 219 8 74 440 23 377 2.22e-71 231
MS.gene36521.t1 AT1G80140 35.143 350 209 6 107 453 2 336 2.31e-70 227
MS.gene36521.t1 AT5G27530 37.222 360 196 7 74 428 52 386 4.74e-70 230
MS.gene36521.t1 AT2G33160 37.624 404 225 11 56 452 19 402 5.29e-70 235
MS.gene36521.t1 AT3G59850 39.377 353 194 10 75 422 23 360 2.52e-69 226
MS.gene36521.t1 AT3G59850 39.377 353 194 10 75 422 67 404 6.56e-69 226
MS.gene36521.t1 AT1G02790 36.872 358 203 8 68 422 45 382 9.74e-69 225
MS.gene36521.t1 AT1G65570 37.500 384 218 12 75 453 31 397 3.04e-68 223
MS.gene36521.t1 AT2G43860 36.979 384 223 11 75 455 33 400 1.93e-67 221
MS.gene36521.t1 AT1G78400 35.733 389 224 10 74 454 34 404 6.52e-64 212
MS.gene36521.t1 AT1G17150 36.104 385 226 8 74 453 32 401 1.02e-63 212
MS.gene36521.t1 AT4G13760 36.134 357 206 9 74 422 30 372 4.19e-63 210
MS.gene36521.t1 AT1G43080 36.415 357 205 9 74 422 30 372 5.64e-63 210
MS.gene36521.t1 AT5G17200 34.063 411 221 12 71 455 35 421 1.54e-62 209
MS.gene36521.t1 AT1G43090 36.695 357 204 9 74 422 30 372 2.37e-62 209
MS.gene36521.t1 AT5G39910 31.328 399 192 10 74 454 35 369 2.85e-61 204
MS.gene36521.t1 AT3G14040 39.660 353 195 9 75 423 71 409 5.08e-61 206
MS.gene36521.t1 AT4G18180 36.413 368 203 8 68 422 32 381 6.39e-61 205
MS.gene36521.t1 AT3G15720 37.356 348 186 11 116 454 2 326 1.44e-60 203
MS.gene36521.t1 AT2G43880 38.851 296 167 5 75 369 31 313 1.90e-60 201
MS.gene36521.t1 AT5G44830 32.840 338 197 6 106 440 8 318 2.10e-60 201
MS.gene36521.t1 AT1G43100 36.134 357 206 9 74 422 30 372 3.09e-60 204
MS.gene36521.t1 AT2G26620 36.056 355 207 9 74 422 30 370 2.19e-59 200
MS.gene36521.t1 AT3G07850 39.093 353 197 9 75 423 70 408 2.58e-59 201
MS.gene36521.t1 AT2G40310 35.955 356 206 9 75 422 31 372 3.29e-59 200
MS.gene36521.t1 AT3G07830 35.503 338 202 7 88 422 34 358 7.28e-59 199
MS.gene36521.t1 AT3G07820 36.337 344 202 8 83 422 27 357 7.48e-59 199
MS.gene36521.t1 AT3G07840 34.971 346 209 7 80 422 26 358 1.05e-58 199
MS.gene36521.t1 AT4G32380 32.976 373 222 8 89 452 41 394 1.18e-58 200
MS.gene36521.t1 AT2G15460 35.475 358 207 9 74 422 30 372 1.41e-58 198
MS.gene36521.t1 AT5G48140 34.911 338 203 7 88 422 34 357 1.59e-58 198
MS.gene36521.t1 AT2G15450 35.196 358 208 9 74 422 30 372 3.48e-58 197
MS.gene36521.t1 AT2G15470 34.916 358 209 9 74 422 30 372 1.30e-56 193
MS.gene36521.t1 AT4G35670 35.185 270 161 3 158 427 2 257 4.08e-53 180
MS.gene36521.t1 AT4G32370 36.860 293 163 6 89 373 49 327 1.26e-52 181
MS.gene36521.t1 AT4G32375 36.667 270 149 4 158 422 13 265 1.82e-50 177
MS.gene36521.t1 AT4G32380 29.459 370 198 8 89 452 41 353 5.96e-46 165
MS.gene36521.t1 AT4G33440 28.363 342 191 12 48 358 52 370 3.00e-23 102
MS.gene36521.t1 AT3G42950 26.842 380 233 12 73 422 64 428 7.44e-21 95.5
MS.gene36521.t1 AT3G62110 26.437 348 210 11 77 393 45 377 6.01e-20 92.8
MS.gene36521.t1 AT3G62110 26.437 348 210 11 77 393 45 377 6.01e-20 92.8
MS.gene36521.t1 AT1G19170 24.627 402 235 14 63 422 75 450 9.43e-20 92.4
MS.gene36521.t1 AT5G41870 26.316 323 189 11 74 364 44 349 4.52e-19 89.7
MS.gene36521.t1 AT5G49215 25.600 375 235 11 77 422 43 402 1.24e-17 85.5
MS.gene36521.t1 AT5G49215 25.600 375 235 11 77 422 45 404 1.26e-17 85.5
MS.gene36521.t1 AT4G23820 25.868 317 191 8 77 364 43 344 1.92e-17 84.7
MS.gene36521.t1 AT3G48950 23.962 313 192 8 77 358 47 344 5.65e-17 83.6
MS.gene36521.t1 AT2G23900 25.641 312 188 10 77 358 58 355 5.22e-16 80.5
MS.gene36521.t1 AT3G61490 24.839 310 192 10 77 358 62 358 1.09e-14 76.6
MS.gene36521.t1 AT3G61490 24.839 310 192 10 77 358 160 456 1.27e-14 76.6
MS.gene36521.t1 AT3G61490 24.839 310 192 10 77 358 47 343 1.34e-14 76.3
MS.gene36521.t1 AT3G61490 24.839 310 192 10 77 358 47 343 1.34e-14 76.3
MS.gene36521.t1 AT3G61490 24.839 310 192 10 77 358 47 343 1.34e-14 76.3
MS.gene36521.t1 AT4G23500 26.537 309 176 11 80 355 73 363 3.30e-13 72.0

Find 109 sgRNAs with CRISPR-Local

Find 183 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACCTCCAATTAATTGTTTA+AGG 0.064758 4.1:-17879266 MS.gene36521:CDS
CAGTTCCACATGATATTTAA+TGG 0.196238 4.1:-17880302 MS.gene36521:CDS
CCTTCCCTTAAACAATTAAT+TGG 0.200232 4.1:+17879261 None:intergenic
TTCATAGACTATTTATTCTT+TGG 0.204045 4.1:-17881864 MS.gene36521:CDS
TTAATTACCATTGCTTATTA+AGG 0.227433 4.1:+17880172 None:intergenic
CCATAAGCATTCCAACAAAA+AGG 0.243640 4.1:+17879309 None:intergenic
GTTTACCCTGTGAGGTTTGC+AGG 0.305698 4.1:+17881117 None:intergenic
GACGAATCCCGAGGATCATT+TGG 0.309383 4.1:+17881688 None:intergenic
GTTACATCATTTGGAGCAAT+TGG 0.311153 4.1:-17881646 MS.gene36521:CDS
TAGTCCATTCACATACAAAT+TGG 0.324642 4.1:+17880343 None:intergenic
AACAATAGTATTGGTCTATA+AGG 0.326820 4.1:+17879520 None:intergenic
TGTAGAGTCAGGAGTTCTTC+TGG 0.355959 4.1:-17881566 MS.gene36521:CDS
TTGATCTCCATTTCCTTCAA+TGG 0.361661 4.1:+17881153 None:intergenic
GAAAGATCACCCACAGTCTC+AGG 0.377658 4.1:+17879234 None:intergenic
GGATCATTTGAAGACTTAGA+AGG 0.381774 4.1:+17881757 None:intergenic
GGTGATGATTGCATTTCAAT+TGG 0.384128 4.1:-17880028 MS.gene36521:CDS
ACATGGATTCCATCAGTGTT+GGG 0.385395 4.1:+17880227 None:intergenic
CTGTACCATTAAAACTCATT+TGG 0.389055 4.1:+17881179 None:intergenic
ACCTCCAATTAATTGTTTAA+GGG 0.389356 4.1:-17879265 MS.gene36521:CDS
TCTGCCTCTGGCCATGAATC+AGG 0.393547 4.1:+17881238 None:intergenic
TGATCCTCGGGATTCGTCTT+CGG 0.398088 4.1:-17881683 MS.gene36521:CDS
TCAGTGTTGGGGCTAAGTTT+TGG 0.401262 4.1:+17880239 None:intergenic
GGCCATGAATCAGGTCCATC+TGG 0.406021 4.1:+17881247 None:intergenic
GTGGTCCTAGCCACGGGATT+AGG 0.416076 4.1:-17879967 MS.gene36521:intron
TTCCATGCTTTCTTGAATGC+AGG 0.417079 4.1:+17881601 None:intergenic
TCACTACAAGCAAAATGAAT+AGG 0.422913 4.1:+17879405 None:intergenic
TGATTTCTGCTATCTGCCTC+TGG 0.424057 4.1:+17881226 None:intergenic
CTTACTTGAAATACTAATCC+TGG 0.430828 4.1:+17881487 None:intergenic
ACAGGAACCATTGAAGGAAA+TGG 0.436139 4.1:-17881160 MS.gene36521:CDS
CCTTTGTCAAACAATAGTAT+TGG 0.440502 4.1:+17879511 None:intergenic
CACCAGATGGACCTGATTCA+TGG 0.440641 4.1:-17881249 MS.gene36521:CDS
AGGAACACTAGGAGAAGAGT+TGG 0.444516 4.1:+17881723 None:intergenic
AACATGGATTCCATCAGTGT+TGG 0.447865 4.1:+17880226 None:intergenic
TCCCGAGGATCATTTGGATA+AGG 0.448420 4.1:+17881694 None:intergenic
AATGGTACAGGAACCATTGA+AGG 0.452512 4.1:-17881166 MS.gene36521:CDS
CCCTTATCCAAATGATCCTC+GGG 0.453352 4.1:-17881695 MS.gene36521:CDS
GCAGCTTGTGCTGTAGAGTC+AGG 0.454931 4.1:-17881577 MS.gene36521:CDS
ATCTTCCCTGCAAACCTCAC+AGG 0.456078 4.1:-17881123 MS.gene36521:CDS
AAAATTTGAGAACATACAAA+TGG 0.456117 4.1:-17879562 MS.gene36521:CDS
GCCATTTGAAGGTGAATTGT+TGG 0.464227 4.1:-17879337 MS.gene36521:CDS
TCCCTTAAACAATTAATTGG+AGG 0.466396 4.1:+17879264 None:intergenic
TTTCATATCTATAGATGATA+CGG 0.467936 4.1:-17880379 MS.gene36521:intron
AAGACATGGCAAGGTGGAAT+GGG 0.468398 4.1:-17879600 MS.gene36521:CDS
TATTTCCACATTTGAAGTCC+CGG 0.469866 4.1:+17880003 None:intergenic
CCCGAGGATCATTTGGATAA+GGG 0.470657 4.1:+17881695 None:intergenic
GTTGAAAACTCACAAGATGT+TGG 0.482732 4.1:-17880206 MS.gene36521:CDS
TAAGTTTGAGACACATGCTT+GGG 0.483234 4.1:+17879671 None:intergenic
TTGAAAACTCACAAGATGTT+GGG 0.483533 4.1:-17880205 MS.gene36521:CDS
TCAGGTGGATGGTATGCTAA+TGG 0.494463 4.1:-17881272 MS.gene36521:intron
AGCTCCAATTTGTATGTGAA+TGG 0.494817 4.1:-17880347 MS.gene36521:CDS
CCAATTAATTGTTTAAGGGA+AGG 0.495839 4.1:-17879261 MS.gene36521:CDS
TAACATGTACTGCTGATGTT+TGG 0.499162 4.1:+17879478 None:intergenic
TTGTACAGCATAGGAAGTCT+TGG 0.499986 4.1:-17879705 MS.gene36521:intron
ACTACTTAATTTGAGAAATG+TGG 0.500752 4.1:-17881830 MS.gene36521:CDS
AAAGACATGGCAAGGTGGAA+TGG 0.501829 4.1:-17879601 MS.gene36521:CDS
ACCCTTATCCAAATGATCCT+CGG 0.501972 4.1:-17881696 MS.gene36521:CDS
TCCAACAATTCACCTTCAAA+TGG 0.505935 4.1:+17879336 None:intergenic
AATACTAATCCTGGTTTGCA+TGG 0.507411 4.1:+17881496 None:intergenic
ACATATTGATCATTACTCCT+TGG 0.515439 4.1:+17880276 None:intergenic
TAACTTGTGGTCCTAGCCAC+GGG 0.522097 4.1:-17879973 MS.gene36521:CDS
ACTTCAAATGTGGAAATAGC+TGG 0.529833 4.1:-17879998 MS.gene36521:CDS
TCATTTGGAGCAATTGGAGA+TGG 0.537985 4.1:-17881640 MS.gene36521:CDS
GTAACTTGTGGTCCTAGCCA+CGG 0.538941 4.1:-17879974 MS.gene36521:CDS
ATTTGAGAAATGTGGATGGA+AGG 0.539093 4.1:-17881822 MS.gene36521:CDS
ATGATATTTAATGGCTGCCA+AGG 0.542871 4.1:-17880293 MS.gene36521:CDS
AACATACAAATGGAGAATGT+AGG 0.544153 4.1:-17879552 MS.gene36521:CDS
ATTTCCACATTTGAAGTCCC+GGG 0.546166 4.1:+17880004 None:intergenic
AAGGAAATGGAGATCAATGG+TGG 0.551216 4.1:-17881147 MS.gene36521:CDS
TTAAGTTTGAGACACATGCT+TGG 0.553610 4.1:+17879670 None:intergenic
AAACTTAGCCCCAACACTGA+TGG 0.554244 4.1:-17880236 MS.gene36521:CDS
GATTGCATTTCAATTGGACC+CGG 0.555852 4.1:-17880022 MS.gene36521:CDS
TACAACTCCTTAATAAGCAA+TGG 0.557489 4.1:-17880179 MS.gene36521:intron
TTGAAGACTTAGAAGGAACA+AGG 0.561772 4.1:+17881764 None:intergenic
CACCTGCATTCAAGAAAGCA+TGG 0.563512 4.1:-17881603 MS.gene36521:CDS
TTGAAATGTAATCAGGTGGA+TGG 0.567449 4.1:-17881283 MS.gene36521:intron
CTTAATTTGAGAAATGTGGA+TGG 0.570049 4.1:-17881826 MS.gene36521:CDS
TTGAAGGAAATGGAGATCAA+TGG 0.574015 4.1:-17881150 MS.gene36521:CDS
TTTACCCTGTGAGGTTTGCA+GGG 0.576342 4.1:+17881118 None:intergenic
TCATTTGGATAAGGGTCAGA+AGG 0.578630 4.1:+17881703 None:intergenic
ATGTACCTAATCCCGTGGCT+AGG 0.592436 4.1:+17879962 None:intergenic
CCAATACTATTGTTTGACAA+AGG 0.596001 4.1:-17879511 MS.gene36521:CDS
GGAAGGTGACCCTGAGACTG+TGG 0.598346 4.1:-17879244 MS.gene36521:CDS
TTAAATATCATGTGGAACTG+AGG 0.598983 4.1:+17880305 None:intergenic
TCTTCCCTGCAAACCTCACA+GGG 0.600082 4.1:-17881122 MS.gene36521:intron
AGGCAGATAGCAGAAATCAA+TGG 0.605028 4.1:-17881222 MS.gene36521:CDS
TGGACCCGGGACTTCAAATG+TGG 0.605054 4.1:-17880008 MS.gene36521:CDS
CTCACAGCTGGACTTCCACA+TGG 0.605942 4.1:+17880862 None:intergenic
TCTTATGACTGTGTCACGGA+CGG 0.610881 4.1:+17879647 None:intergenic
CTAAGAATAAAGACATGGCA+AGG 0.613229 4.1:-17879609 MS.gene36521:CDS
GGTATGCTAATGGCACCAGA+TGG 0.619104 4.1:-17881262 MS.gene36521:CDS
GGCAGCCATTAAATATCATG+TGG 0.622918 4.1:+17880297 None:intergenic
CATGGATTCCATCAGTGTTG+GGG 0.624775 4.1:+17880228 None:intergenic
TAAGGGTCAGAAGGAACACT+AGG 0.625203 4.1:+17881712 None:intergenic
ATTGCATTTCAATTGGACCC+GGG 0.630537 4.1:-17880021 MS.gene36521:CDS
TGAAGACTTAGAAGGAACAA+GGG 0.632788 4.1:+17881765 None:intergenic
ACACTAGGAGAAGAGTTGGA+AGG 0.640153 4.1:+17881727 None:intergenic
AAAGATCACCCACAGTCTCA+GGG 0.643345 4.1:+17879235 None:intergenic
ATGTAATGTACCTAATCCCG+TGG 0.645529 4.1:+17879957 None:intergenic
TGGACCTGATTCATGGCCAG+AGG 0.650317 4.1:-17881242 MS.gene36521:CDS
GAAATAGCTGGTGTAACTTG+TGG 0.654498 4.1:-17879986 MS.gene36521:CDS
GTCATAAGAGAATCAGACAA+TGG 0.654639 4.1:-17879633 MS.gene36521:CDS
ATTCTCTTATGACTGTGTCA+CGG 0.659505 4.1:+17879643 None:intergenic
AAAACTTTGTCAGGACCATG+TGG 0.660723 4.1:-17880877 MS.gene36521:CDS
AGGAAATGGAGATCAATGGT+GGG 0.664389 4.1:-17881146 MS.gene36521:CDS
TACAAACATAACACTCTCTG+AGG 0.682981 4.1:-17879370 MS.gene36521:CDS
AGAATAAAGACATGGCAAGG+TGG 0.690276 4.1:-17879606 MS.gene36521:CDS
CAGTCCGAAGACGAATCCCG+AGG 0.710358 4.1:+17881679 None:intergenic
ATGGACTAAGAATAAAGACA+TGG 0.715833 4.1:-17879614 MS.gene36521:CDS
GAAGGTGACCCTGAGACTGT+GGG 0.722175 4.1:-17879243 MS.gene36521:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAAAATAAATGATATATA+TGG + chr4.1:17879654-17879673 None:intergenic 10.0%
!! ATTCTATATGTAATTAATAT+AGG - chr4.1:17880277-17880296 MS.gene36521:CDS 10.0%
!! TCTATAAATAAATATTTACA+TGG + chr4.1:17880024-17880043 None:intergenic 10.0%
!! TTCTATATGTAATTAATATA+GGG - chr4.1:17880278-17880297 MS.gene36521:CDS 10.0%
!!! ATATCCTATTTTTATTTTTA+AGG - chr4.1:17880591-17880610 MS.gene36521:intron 10.0%
!!! ATATTTTGAATAGAAAAATT+AGG + chr4.1:17881236-17881255 None:intergenic 10.0%
!!! TAAAATTCGTTTTATTTTTA+GGG - chr4.1:17880622-17880641 MS.gene36521:intron 10.0%
!!! TTAAAATTCGTTTTATTTTT+AGG - chr4.1:17880621-17880640 MS.gene36521:intron 10.0%
!! ATGTCAAAATTATATCAATT+AGG - chr4.1:17880532-17880551 MS.gene36521:intron 15.0%
!! TAGTCCTTAAAAATAAAAAT+AGG + chr4.1:17880598-17880617 None:intergenic 15.0%
!! TCAACTTAATAAAATATGAT+TGG - chr4.1:17879708-17879727 MS.gene36521:intron 15.0%
!! TGTCAAAATTATATCAATTA+GGG - chr4.1:17880533-17880552 MS.gene36521:intron 15.0%
!!! AAAATTCGTTTTATTTTTAG+GGG - chr4.1:17880623-17880642 MS.gene36521:intron 15.0%
!!! AAAATTTTCCATTTTGTTTT+TGG - chr4.1:17880386-17880405 MS.gene36521:intron 15.0%
!! AAAATTTGAGAACATACAAA+TGG - chr4.1:17881524-17881543 MS.gene36521:CDS 20.0%
!! AACAAAATTGAAATGTAATC+AGG - chr4.1:17879796-17879815 MS.gene36521:intron 20.0%
!! AACATAGAAACAATAAAACT+GGG + chr4.1:17880968-17880987 None:intergenic 20.0%
!! ACATTAGCATAAACATAAAT+TGG + chr4.1:17880422-17880441 None:intergenic 20.0%
!! ATAAACATAAATTGGTCATT+AGG + chr4.1:17880414-17880433 None:intergenic 20.0%
!! GACATTTCTTTAATATAACA+TGG + chr4.1:17880506-17880525 None:intergenic 20.0%
!! TAAACATAAATTGGTCATTA+GGG + chr4.1:17880413-17880432 None:intergenic 20.0%
!! TAACATAGAAACAATAAAAC+TGG + chr4.1:17880969-17880988 None:intergenic 20.0%
!! TAATAAGCAATGGTAATTAA+TGG - chr4.1:17880917-17880936 MS.gene36521:intron 20.0%
!! TTAATTACCATTGCTTATTA+AGG + chr4.1:17880917-17880936 None:intergenic 20.0%
!! TTACTATTGACAAAATTTAG+AGG - chr4.1:17881255-17881274 MS.gene36521:CDS 20.0%
!! TTCATAGACTATTTATTCTT+TGG - chr4.1:17879222-17879241 MS.gene36521:CDS 20.0%
!! TTTCATATCTATAGATGATA+CGG - chr4.1:17880707-17880726 MS.gene36521:intron 20.0%
!!! AAAATGCCAAGTTTTATTTT+TGG + chr4.1:17880174-17880193 None:intergenic 20.0%
!!! AAATGAAACAAAAATTTTCG+AGG - chr4.1:17880561-17880580 MS.gene36521:intron 20.0%
!!! AATGAAACAAAAATTTTCGA+GGG - chr4.1:17880562-17880581 MS.gene36521:intron 20.0%
!!! ACAAAATGGAAAATTTTGAT+AGG + chr4.1:17880383-17880402 None:intergenic 20.0%
!!! ACATCTCGTTTTTATTTTTA+TGG + chr4.1:17880354-17880373 None:intergenic 20.0%
!!! ATAGTGTAATTTGTTTTACA+CGG - chr4.1:17880078-17880097 MS.gene36521:intron 20.0%
!!! ATCACTTCAATTATTTTTTC+AGG - chr4.1:17879566-17879585 MS.gene36521:CDS 20.0%
!!! ATCATAAAAGTGTAATTTTC+TGG + chr4.1:17879548-17879567 None:intergenic 20.0%
! AAAATTGAAATGTAATCAGG+TGG - chr4.1:17879799-17879818 MS.gene36521:intron 25.0%
! ACCTCCAATTAATTGTTTAA+GGG - chr4.1:17881821-17881840 MS.gene36521:CDS 25.0%
! CACTAACCAAAAATAAAACT+TGG - chr4.1:17880165-17880184 MS.gene36521:intron 25.0%
! CTGGGTAAGTAAATTAATAT+TGG + chr4.1:17880950-17880969 None:intergenic 25.0%
! TACCTCCAATTAATTGTTTA+AGG - chr4.1:17881820-17881839 MS.gene36521:CDS 25.0%
! TGAATAGAAAAATTAGGATC+TGG + chr4.1:17881230-17881249 None:intergenic 25.0%
! TTAGAGGTGAAATATGAAAT+CGG - chr4.1:17881271-17881290 MS.gene36521:CDS 25.0%
! TTTAATATAACATGGACTCA+GGG + chr4.1:17880498-17880517 None:intergenic 25.0%
!! AAATAAAACTTGGCATTTTC+AGG - chr4.1:17880175-17880194 MS.gene36521:intron 25.0%
!! AATAAAACTTGGCATTTTCA+GGG - chr4.1:17880176-17880195 MS.gene36521:intron 25.0%
!! ACTACTTAATTTGAGAAATG+TGG - chr4.1:17879256-17879275 MS.gene36521:CDS 25.0%
!! ATTTTCGATGTTACATCATT+TGG - chr4.1:17879431-17879450 MS.gene36521:CDS 25.0%
!! TTGTTAATTTTGTACAGCAT+AGG - chr4.1:17881372-17881391 MS.gene36521:intron 25.0%
!!! AACAATAGTATTGGTCTATA+AGG + chr4.1:17881569-17881588 None:intergenic 25.0%
!!! AACATGATTTGATTTTGAAC+AGG - chr4.1:17881037-17881056 MS.gene36521:intron 25.0%
!!! CAAGTTTTATTTTTGGTTAG+TGG + chr4.1:17880167-17880186 None:intergenic 25.0%
AACATACAAATGGAGAATGT+AGG - chr4.1:17881534-17881553 MS.gene36521:CDS 30.0%
ACATATTGATCATTACTCCT+TGG + chr4.1:17880813-17880832 None:intergenic 30.0%
ACTAAACTTCGTGACAAAAT+TGG + chr4.1:17881300-17881319 None:intergenic 30.0%
ATTAGGGACCAAAAACAAAA+TGG + chr4.1:17880397-17880416 None:intergenic 30.0%
ATTGGAAGTAAGTGTAAAAC+TGG - chr4.1:17879726-17879745 MS.gene36521:intron 30.0%
CAGTTCCACATGATATTTAA+TGG - chr4.1:17880784-17880803 MS.gene36521:intron 30.0%
CATAAGCATTCCAACAAAAA+GGG + chr4.1:17881779-17881798 None:intergenic 30.0%
CCAATACTATTGTTTGACAA+AGG - chr4.1:17881575-17881594 MS.gene36521:CDS 30.0%
CCAATTAATTGTTTAAGGGA+AGG - chr4.1:17881825-17881844 MS.gene36521:CDS 30.0%
CCTTCCCTTAAACAATTAAT+TGG + chr4.1:17881828-17881847 None:intergenic 30.0%
CCTTTGTCAAACAATAGTAT+TGG + chr4.1:17881578-17881597 None:intergenic 30.0%
CTGTACCATTAAAACTCATT+TGG + chr4.1:17879910-17879929 None:intergenic 30.0%
CTTACTTGAAATACTAATCC+TGG + chr4.1:17879602-17879621 None:intergenic 30.0%
CTTTAATATAACATGGACTC+AGG + chr4.1:17880499-17880518 None:intergenic 30.0%
GCTGCTTGCTTATATAAAAA+TGG + chr4.1:17881007-17881026 None:intergenic 30.0%
GTGTCCTACAAAAACATTAA+GGG - chr4.1:17881636-17881655 MS.gene36521:CDS 30.0%
TACAACTCCTTAATAAGCAA+TGG - chr4.1:17880907-17880926 MS.gene36521:intron 30.0%
TAGTCCATTCACATACAAAT+TGG + chr4.1:17880746-17880765 None:intergenic 30.0%
TATTTAATGACTGAGCTGAA+TGG + chr4.1:17880255-17880274 None:intergenic 30.0%
TCACTACAAGCAAAATGAAT+AGG + chr4.1:17881684-17881703 None:intergenic 30.0%
TCCCTTAAACAATTAATTGG+AGG + chr4.1:17881825-17881844 None:intergenic 30.0%
TGTGTCCTACAAAAACATTA+AGG - chr4.1:17881635-17881654 MS.gene36521:CDS 30.0%
TTAAATATCATGTGGAACTG+AGG + chr4.1:17880784-17880803 None:intergenic 30.0%
TTAATATAACATGGACTCAG+GGG + chr4.1:17880497-17880516 None:intergenic 30.0%
TTGAAAACTCACAAGATGTT+GGG - chr4.1:17880881-17880900 MS.gene36521:CDS 30.0%
! ATGGACTAAGAATAAAGACA+TGG - chr4.1:17881472-17881491 MS.gene36521:intron 30.0%
! CTTAATTTGAGAAATGTGGA+TGG - chr4.1:17879260-17879279 MS.gene36521:CDS 30.0%
!! AGTACCCTTAATGTTTTTGT+AGG + chr4.1:17881643-17881662 None:intergenic 30.0%
!! CAAATGAGTTTTAATGGTAC+AGG - chr4.1:17879908-17879927 MS.gene36521:intron 30.0%
!! CTTGACCAAATGAGTTTTAA+TGG - chr4.1:17879902-17879921 MS.gene36521:intron 30.0%
ACTTCAAATGTGGAAATAGC+TGG - chr4.1:17881088-17881107 MS.gene36521:intron 35.0%
AGCTCCAATTTGTATGTGAA+TGG - chr4.1:17880739-17880758 MS.gene36521:intron 35.0%
ATGATATTTAATGGCTGCCA+AGG - chr4.1:17880793-17880812 MS.gene36521:intron 35.0%
ATTCTCTTATGACTGTGTCA+CGG + chr4.1:17881446-17881465 None:intergenic 35.0%
ATTTGAGAAATGTGGATGGA+AGG - chr4.1:17879264-17879283 MS.gene36521:CDS 35.0%
CCATAAGCATTCCAACAAAA+AGG + chr4.1:17881780-17881799 None:intergenic 35.0%
CTAAGAATAAAGACATGGCA+AGG - chr4.1:17881477-17881496 MS.gene36521:intron 35.0%
GGATCATTTGAAGACTTAGA+AGG + chr4.1:17879332-17879351 None:intergenic 35.0%
GGTGATGATTGCATTTCAAT+TGG - chr4.1:17881058-17881077 MS.gene36521:intron 35.0%
GTCATAAGAGAATCAGACAA+TGG - chr4.1:17881453-17881472 MS.gene36521:intron 35.0%
GTTGAAAACTCACAAGATGT+TGG - chr4.1:17880880-17880899 MS.gene36521:CDS 35.0%
TAACATGTACTGCTGATGTT+TGG + chr4.1:17881611-17881630 None:intergenic 35.0%
TAATATAACATGGACTCAGG+GGG + chr4.1:17880496-17880515 None:intergenic 35.0%
TACAAACATAACACTCTCTG+AGG - chr4.1:17881716-17881735 MS.gene36521:CDS 35.0%
TCAAAAAAGTTTACCCTGTG+AGG + chr4.1:17879980-17879999 None:intergenic 35.0%
TCCAACAATTCACCTTCAAA+TGG + chr4.1:17881753-17881772 None:intergenic 35.0%
TGAAGACTTAGAAGGAACAA+GGG + chr4.1:17879324-17879343 None:intergenic 35.0%
TTGAAATGTAATCAGGTGGA+TGG - chr4.1:17879803-17879822 MS.gene36521:intron 35.0%
TTGAAGACTTAGAAGGAACA+AGG + chr4.1:17879325-17879344 None:intergenic 35.0%
TTGAAGGAAATGGAGATCAA+TGG - chr4.1:17879936-17879955 MS.gene36521:intron 35.0%
TTGATCTCCATTTCCTTCAA+TGG + chr4.1:17879936-17879955 None:intergenic 35.0%
! AATACTAATCCTGGTTTGCA+TGG + chr4.1:17879593-17879612 None:intergenic 35.0%
! CATCTTGTGAGTTTTCAACA+TGG + chr4.1:17880879-17880898 None:intergenic 35.0%
! CCTTTTTGTTGGAATGCTTA+TGG - chr4.1:17881777-17881796 MS.gene36521:CDS 35.0%
! GGAAGATACTTTCTTTTGCT+TGG + chr4.1:17879303-17879322 None:intergenic 35.0%
! GTTACATCATTTGGAGCAAT+TGG - chr4.1:17879440-17879459 MS.gene36521:CDS 35.0%
! TAAGTTTGAGACACATGCTT+GGG + chr4.1:17881418-17881437 None:intergenic 35.0%
! TATTTCCACATTTGAAGTCC+CGG + chr4.1:17881086-17881105 None:intergenic 35.0%
! TTAAGTTTGAGACACATGCT+TGG + chr4.1:17881419-17881438 None:intergenic 35.0%
AAAACTTTGTCAGGACCATG+TGG - chr4.1:17880209-17880228 MS.gene36521:CDS 40.0%
AACATGGATTCCATCAGTGT+TGG + chr4.1:17880863-17880882 None:intergenic 40.0%
AAGGAAATGGAGATCAATGG+TGG - chr4.1:17879939-17879958 MS.gene36521:intron 40.0%
AATGGTACAGGAACCATTGA+AGG - chr4.1:17879920-17879939 MS.gene36521:intron 40.0%
ACAGGAACCATTGAAGGAAA+TGG - chr4.1:17879926-17879945 MS.gene36521:intron 40.0%
ACATGGATTCCATCAGTGTT+GGG + chr4.1:17880862-17880881 None:intergenic 40.0%
ACCCTTATCCAAATGATCCT+CGG - chr4.1:17879390-17879409 MS.gene36521:CDS 40.0%
AGAATAAAGACATGGCAAGG+TGG - chr4.1:17881480-17881499 MS.gene36521:intron 40.0%
AGGAAATGGAGATCAATGGT+GGG - chr4.1:17879940-17879959 MS.gene36521:intron 40.0%
AGGCAGATAGCAGAAATCAA+TGG - chr4.1:17879864-17879883 MS.gene36521:intron 40.0%
ATGTAATGTACCTAATCCCG+TGG + chr4.1:17881132-17881151 None:intergenic 40.0%
ATTGCATTTCAATTGGACCC+GGG - chr4.1:17881065-17881084 MS.gene36521:intron 40.0%
CACTACCAAGTGAAACAACA+TGG - chr4.1:17881192-17881211 MS.gene36521:CDS 40.0%
CCAGATGGAAAAACTTTGTC+AGG - chr4.1:17880200-17880219 MS.gene36521:CDS 40.0%
GAAATAGCTGGTGTAACTTG+TGG - chr4.1:17881100-17881119 MS.gene36521:intron 40.0%
GATTGCATTTCAATTGGACC+CGG - chr4.1:17881064-17881083 MS.gene36521:intron 40.0%
GCCATTTGAAGGTGAATTGT+TGG - chr4.1:17881749-17881768 MS.gene36521:CDS 40.0%
GGCAGCCATTAAATATCATG+TGG + chr4.1:17880792-17880811 None:intergenic 40.0%
TCATTTGGATAAGGGTCAGA+AGG + chr4.1:17879386-17879405 None:intergenic 40.0%
TTCCATGCTTTCTTGAATGC+AGG + chr4.1:17879488-17879507 None:intergenic 40.0%
TTGTACAGCATAGGAAGTCT+TGG - chr4.1:17881381-17881400 MS.gene36521:intron 40.0%
! ATTTCCACATTTGAAGTCCC+GGG + chr4.1:17881085-17881104 None:intergenic 40.0%
! CCTGACAAAGTTTTTCCATC+TGG + chr4.1:17880203-17880222 None:intergenic 40.0%
! GAAGAGTTGGAAGGATTTTC+TGG + chr4.1:17879353-17879372 None:intergenic 40.0%
! TCATTTGGAGCAATTGGAGA+TGG - chr4.1:17879446-17879465 MS.gene36521:CDS 40.0%
! TGTTGGATGACCCTTTTTGT+TGG - chr4.1:17881766-17881785 MS.gene36521:CDS 40.0%
!! GCAAACCATGTTGTTTCACT+TGG + chr4.1:17881200-17881219 None:intergenic 40.0%
!! GCTAAGTTTTGGTGCAGAAA+TGG + chr4.1:17880839-17880858 None:intergenic 40.0%
!! GTTGAGCTTTTGCCATTTGA+AGG - chr4.1:17881738-17881757 MS.gene36521:CDS 40.0%
!! TGAATGGTTTTACTCACAGC+TGG + chr4.1:17880239-17880258 None:intergenic 40.0%
!!! TCTTTTGCTTGGTGTGAAAG+TGG + chr4.1:17879292-17879311 None:intergenic 40.0%
AAACTTAGCCCCAACACTGA+TGG - chr4.1:17880850-17880869 MS.gene36521:intron 45.0%
AAAGACATGGCAAGGTGGAA+TGG - chr4.1:17881485-17881504 MS.gene36521:intron 45.0%
AAAGATCACCCACAGTCTCA+GGG + chr4.1:17881854-17881873 None:intergenic 45.0%
AAGACATGGCAAGGTGGAAT+GGG - chr4.1:17881486-17881505 MS.gene36521:intron 45.0%
ACACTAGGAGAAGAGTTGGA+AGG + chr4.1:17879362-17879381 None:intergenic 45.0%
AGGAACACTAGGAGAAGAGT+TGG + chr4.1:17879366-17879385 None:intergenic 45.0%
CACCTGCATTCAAGAAAGCA+TGG - chr4.1:17879483-17879502 MS.gene36521:CDS 45.0%
CATGGATTCCATCAGTGTTG+GGG + chr4.1:17880861-17880880 None:intergenic 45.0%
CCCGAGGATCATTTGGATAA+GGG + chr4.1:17879394-17879413 None:intergenic 45.0%
CCCTTATCCAAATGATCCTC+GGG - chr4.1:17879391-17879410 MS.gene36521:CDS 45.0%
TAAGGGTCAGAAGGAACACT+AGG + chr4.1:17879377-17879396 None:intergenic 45.0%
TCAGGTGGATGGTATGCTAA+TGG - chr4.1:17879814-17879833 MS.gene36521:intron 45.0%
TCCCGAGGATCATTTGGATA+AGG + chr4.1:17879395-17879414 None:intergenic 45.0%
TCTTATGACTGTGTCACGGA+CGG + chr4.1:17881442-17881461 None:intergenic 45.0%
TGATTTCTGCTATCTGCCTC+TGG + chr4.1:17879863-17879882 None:intergenic 45.0%
TGTAGAGTCAGGAGTTCTTC+TGG - chr4.1:17879520-17879539 MS.gene36521:CDS 45.0%
TTTACCCTGTGAGGTTTGCA+GGG + chr4.1:17879971-17879990 None:intergenic 45.0%
!! TCAGTGTTGGGGCTAAGTTT+TGG + chr4.1:17880850-17880869 None:intergenic 45.0%
!!! TTTTCAGGTCCATGCAAACC+AGG - chr4.1:17879581-17879600 MS.gene36521:CDS 45.0%
ATCTTCCCTGCAAACCTCAC+AGG - chr4.1:17879963-17879982 MS.gene36521:intron 50.0%
ATGTACCTAATCCCGTGGCT+AGG + chr4.1:17881127-17881146 None:intergenic 50.0%
CACCAGATGGACCTGATTCA+TGG - chr4.1:17879837-17879856 MS.gene36521:intron 50.0%
GAAAGATCACCCACAGTCTC+AGG + chr4.1:17881855-17881874 None:intergenic 50.0%
GACGAATCCCGAGGATCATT+TGG + chr4.1:17879401-17879420 None:intergenic 50.0%
GGTATGCTAATGGCACCAGA+TGG - chr4.1:17879824-17879843 MS.gene36521:intron 50.0%
GTTTACCCTGTGAGGTTTGC+AGG + chr4.1:17879972-17879991 None:intergenic 50.0%
TATAACATGGACTCAGGGGG+TGG + chr4.1:17880493-17880512 None:intergenic 50.0%
TCTTCCCTGCAAACCTCACA+GGG - chr4.1:17879964-17879983 MS.gene36521:intron 50.0%
TGATCCTCGGGATTCGTCTT+CGG - chr4.1:17879403-17879422 MS.gene36521:CDS 50.0%
! GTAACTTGTGGTCCTAGCCA+CGG - chr4.1:17881112-17881131 MS.gene36521:intron 50.0%
! TAACTTGTGGTCCTAGCCAC+GGG - chr4.1:17881113-17881132 MS.gene36521:intron 50.0%
!! TGGCATTTTCAGGGTCCAGA+TGG - chr4.1:17880185-17880204 MS.gene36521:CDS 50.0%
CTCACAGCTGGACTTCCACA+TGG + chr4.1:17880227-17880246 None:intergenic 55.0%
GAAGGTGACCCTGAGACTGT+GGG - chr4.1:17881843-17881862 MS.gene36521:CDS 55.0%
GCAGCTTGTGCTGTAGAGTC+AGG - chr4.1:17879509-17879528 MS.gene36521:CDS 55.0%
GGCCATGAATCAGGTCCATC+TGG + chr4.1:17879842-17879861 None:intergenic 55.0%
TCTGCCTCTGGCCATGAATC+AGG + chr4.1:17879851-17879870 None:intergenic 55.0%
TGGACCCGGGACTTCAAATG+TGG - chr4.1:17881078-17881097 MS.gene36521:intron 55.0%
TGGACCTGATTCATGGCCAG+AGG - chr4.1:17879844-17879863 MS.gene36521:intron 55.0%
CAGTCCGAAGACGAATCCCG+AGG + chr4.1:17879410-17879429 None:intergenic 60.0%
GGAAGGTGACCCTGAGACTG+TGG - chr4.1:17881842-17881861 MS.gene36521:CDS 60.0%
GTGGTCCTAGCCACGGGATT+AGG - chr4.1:17881119-17881138 MS.gene36521:intron 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 17879212 17881896 17879212 ID=MS.gene36521
chr4.1 mRNA 17879212 17881896 17879212 ID=MS.gene36521.t1;Parent=MS.gene36521
chr4.1 exon 17881492 17881896 17881492 ID=MS.gene36521.t1.exon1;Parent=MS.gene36521.t1
chr4.1 CDS 17881492 17881896 17881492 ID=cds.MS.gene36521.t1;Parent=MS.gene36521.t1
chr4.1 exon 17881123 17881290 17881123 ID=MS.gene36521.t1.exon2;Parent=MS.gene36521.t1
chr4.1 CDS 17881123 17881290 17881123 ID=cds.MS.gene36521.t1;Parent=MS.gene36521.t1
chr4.1 exon 17880867 17880911 17880867 ID=MS.gene36521.t1.exon3;Parent=MS.gene36521.t1
chr4.1 CDS 17880867 17880911 17880867 ID=cds.MS.gene36521.t1;Parent=MS.gene36521.t1
chr4.1 exon 17880180 17880387 17880180 ID=MS.gene36521.t1.exon4;Parent=MS.gene36521.t1
chr4.1 CDS 17880180 17880387 17880180 ID=cds.MS.gene36521.t1;Parent=MS.gene36521.t1
chr4.1 exon 17879968 17880049 17879968 ID=MS.gene36521.t1.exon5;Parent=MS.gene36521.t1
chr4.1 CDS 17879968 17880049 17879968 ID=cds.MS.gene36521.t1;Parent=MS.gene36521.t1
chr4.1 exon 17879212 17879719 17879212 ID=MS.gene36521.t1.exon6;Parent=MS.gene36521.t1
chr4.1 CDS 17879212 17879719 17879212 ID=cds.MS.gene36521.t1;Parent=MS.gene36521.t1
Gene Sequence

>MS.gene36521

ATGGATCTCATTCATAGACTATTTATTCTTTGGATTACATTTATACTACTTAATTTGAGAAATGTGGATGGAAGGAACCACTTTCACACCAAGCAAAAGAAAGTATCTTCCCTTGTTCCTTCTAAGTCTTCAAATGATCCAGAAAATCCTTCCAACTCTTCTCCTAGTGTTCCTTCTGACCCTTATCCAAATGATCCTCGGGATTCGTCTTCGGACTGTATTTTCGATGTTACATCATTTGGAGCAATTGGAGATGGTTCAGCTGATGACACACCTGCATTCAAGAAAGCATGGAAAGCAGCTTGTGCTGTAGAGTCAGGAGTTCTTCTGGTTCCAGAAAATTACACTTTTATGATCACTTCAATTATTTTTTCAGGTCCATGCAAACCAGGATTAGTATTTCAAGTAAGTAAGATAACTTTTTCAATTTCTAATTTCACCATATATATCATTTATTTTGTTACAAACAAATTATTATTTCTTAAATGCATAAATATCAACTTAATAAAATATGATTGGAAGTAAGTGTAAAACTGGTTTAACGTTAGTGCGAATTCTTACTATTATAATAATATGAAATTATTAACAAAATTGAAATGTAATCAGGTGGATGGTATGCTAATGGCACCAGATGGACCTGATTCATGGCCAGAGGCAGATAGCAGAAATCAATGGCTTGTGTTCTATAAACTTGACCAAATGAGTTTTAATGGTACAGGAACCATTGAAGGAAATGGAGATCAATGGTGGGATCTTCCCTGCAAACCTCACAGGGTAAACTTTTTTGAACATTATTCAAGCATATCTCACCATGTAAATATTTATTTATAGAGATATTTTAAAAACTAATTTAAGAAAGTGACATTATAGTGTAATTTGTTTTACACGGTGAATGTGTACAAATTAATTTCTATTACTATTGTTGTATGCATCTAATGTTGATTGATACACACCACTAACCAAAAATAAAACTTGGCATTTTCAGGGTCCAGATGGAAAAACTTTGTCAGGACCATGTGGAAGTCCAGCTGTGAGTAAAACCATTCAGCTCAGTCATTAAATAACATTCTATATGTAATTAATATAGGGTGAAATAAATTTTTATATCGTAAAAATATAGAAAATTTTGATTATATAGTCCATAAAAATAAAAACGAGATGTTTTCTTCCTATCAAAATTTTCCATTTTGTTTTTGGTCCCTAATGACCAATTTATGTTTATGCTAATGTCAACATAATTATGATCATTTTTATGTTTCAGATGTATTCAATGTATCTCCACCCCCTGAGTCCATGTTATATTAAAGAAATGTCAAAAACATGTCAAAATTATATCAATTAGGGACAAAAAATGAAACAAAAATTTTCGAGGGACAAAAATATCCTATTTTTATTTTTAAGGACTAAAATTAAAATTCGTTTTATTTTTAGGGGAAGAAAAACTTATTTAATTCAATAATATATTACTAGCTAGTGTCTTAAAGTTGTTTTTAATTTTCATATCTATAGATGATACGGTTCTTCATGAGCTCCAATTTGTATGTGAATGGACTAAAAATTCAAAACAGTCCTCAGTTCCACATGATATTTAATGGCTGCCAAGGAGTAATGATCAATATGTTATCCATTTCTGCACCAAAACTTAGCCCCAACACTGATGGAATCCATGTTGAAAACTCACAAGATGTTGGGATATACAACTCCTTAATAAGCAATGGTAATTAATGGCTATAATCCAATATTAATTTACTTACCCAGTTTTATTGTTTCTATGTTAATTTTATTAATTTTACCATTTTTATATAAGCAAGCAGCTTAATATAATAACATGATTTGATTTTGAACAGGTGATGATTGCATTTCAATTGGACCCGGGACTTCAAATGTGGAAATAGCTGGTGTAACTTGTGGTCCTAGCCACGGGATTAGGTACATTACATAATCTATTCTTGTTTTGTTGATTAATAAAAAAATTAGTGTCACTACCAAGTGAAACAACATGGTTTGCAACAATACCAGATCCTAATTTTTCTATTCAAAATATTACTATTGACAAAATTTAGAGGTGAAATATGAAATCGGTCTCCAATTTTGTCACGAAGTTTAGTTTAGTTTAATTTTCTGTAGTACATATCAGCATGAGTCACTAAACTTAATGTTTTGTTAATTTTGTACAGCATAGGAAGTCTTGGTGTGCACAATTCCCAAGCATGTGTCTCAAACTTAACCGTCCGTGACACAGTCATAAGAGAATCAGACAATGGACTAAGAATAAAGACATGGCAAGGTGGAATGGGATCTGTGTCAAATTTAAAATTTGAGAACATACAAATGGAGAATGTAGGAAACTGCATCCTTATAGACCAATACTATTGTTTGACAAAGGAATGTCTTAACCAAACATCAGCAGTACATGTTAATGATGTGTCCTACAAAAACATTAAGGGTACTTATGATGTTAGAACTGCTCCTATTCATTTTGCTTGTAGTGACACTGTTGCTTGTACAAACATAACACTCTCTGAGGTTGAGCTTTTGCCATTTGAAGGTGAATTGTTGGATGACCCTTTTTGTTGGAATGCTTATGGAGATCAAGAGACTTTGACTATACCTCCAATTAATTGTTTAAGGGAAGGTGACCCTGAGACTGTGGGTGATCTTTCTACATATCAATGTAGTAATTAG
Protein sequence

>MS.gene36521.t1

MDLIHRLFILWITFILLNLRNVDGRNHFHTKQKKVSSLVPSKSSNDPENPSNSSPSVPSDPYPNDPRDSSSDCIFDVTSFGAIGDGSADDTPAFKKAWKAACAVESGVLLVPENYTFMITSIIFSGPCKPGLVFQVDGMLMAPDGPDSWPEADSRNQWLVFYKLDQMSFNGTGTIEGNGDQWWDLPCKPHRGPDGKTLSGPCGSPAMIRFFMSSNLYVNGLKIQNSPQFHMIFNGCQGVMINMLSISAPKLSPNTDGIHVENSQDVGIYNSLISNGDDCISIGPGTSNVEIAGVTCGPSHGISIGSLGVHNSQACVSNLTVRDTVIRESDNGLRIKTWQGGMGSVSNLKFENIQMENVGNCILIDQYYCLTKECLNQTSAVHVNDVSYKNIKGTYDVRTAPIHFACSDTVACTNITLSEVELLPFEGELLDDPFCWNAYGDQETLTIPPINCLREGDPETVGDLSTYQCSN