Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36536.t1 | KEH24003.1 | 87.1 | 178 | 21 | 2 | 1 | 177 | 175 | 351 | 1.50E-78 | 302.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36536.t1 | A0A072U4F2 | 87.1 | 178 | 21 | 2 | 1 | 177 | 175 | 351 | 1.1e-78 | 302.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055356 | MS.gene36536 | 0.800874 | 1.17E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36536.t1 | MTR_7g100328 | 87.079 | 178 | 21 | 2 | 1 | 177 | 175 | 351 | 7.45e-108 | 312 |
| MS.gene36536.t1 | MTR_1g067600 | 43.151 | 146 | 77 | 3 | 14 | 158 | 198 | 338 | 1.34e-33 | 122 |
| MS.gene36536.t1 | MTR_3g095070 | 33.526 | 173 | 107 | 4 | 1 | 171 | 158 | 324 | 1.28e-22 | 92.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36536.t1 | AT2G37400 | 54.335 | 173 | 73 | 3 | 2 | 173 | 165 | 332 | 1.74e-55 | 178 |
| MS.gene36536.t1 | AT3G53560 | 54.913 | 173 | 72 | 3 | 2 | 173 | 165 | 332 | 3.83e-54 | 175 |
| MS.gene36536.t1 | AT3G53560 | 54.913 | 173 | 72 | 3 | 2 | 173 | 213 | 380 | 8.50e-54 | 176 |
| MS.gene36536.t1 | AT5G02590 | 50.617 | 162 | 73 | 4 | 2 | 162 | 164 | 319 | 2.72e-45 | 152 |
| MS.gene36536.t1 | AT3G09490 | 34.783 | 161 | 101 | 1 | 3 | 163 | 149 | 305 | 1.58e-26 | 103 |
| MS.gene36536.t1 | AT4G39470 | 31.210 | 157 | 100 | 3 | 1 | 156 | 167 | 316 | 6.95e-22 | 90.9 |
| MS.gene36536.t1 | AT3G18420 | 30.539 | 167 | 103 | 5 | 2 | 164 | 153 | 310 | 2.49e-17 | 78.2 |
Find 34 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAAGAAGGTTTCGGATGTT+AGG | 0.266026 | 7.1:+7484186 | MS.gene36536:CDS |
| CTTCGAGTTGAAGCATTTCA+TGG | 0.282568 | 7.1:+7484065 | MS.gene36536:CDS |
| TCATCAAAATACTCTTTATA+AGG | 0.300941 | 7.1:-7484413 | None:intergenic |
| AGAGGCGCATAAAGCTTATC+AGG | 0.310700 | 7.1:+7484259 | MS.gene36536:CDS |
| ACAATTTCTTAGCAGATTCA+TGG | 0.328436 | 7.1:-7484016 | None:intergenic |
| GTTATTGGTTGCTCAGATTA+GGG | 0.339072 | 7.1:+7484217 | MS.gene36536:CDS |
| TCTTTATAAGGATGATTCTT+CGG | 0.343610 | 7.1:-7484401 | None:intergenic |
| GGTTATTGGTTGCTCAGATT+AGG | 0.351325 | 7.1:+7484216 | MS.gene36536:CDS |
| CTTTATAAGGATGATTCTTC+GGG | 0.354190 | 7.1:-7484400 | None:intergenic |
| GCATAAAGCTTATCAGGATT+TGG | 0.366772 | 7.1:+7484265 | MS.gene36536:CDS |
| TGGACTTGTGATGGCGAGTT+CGG | 0.402706 | 7.1:+7484085 | MS.gene36536:CDS |
| GAAGAAGGTTTCGGATGTTA+GGG | 0.426707 | 7.1:+7484187 | MS.gene36536:CDS |
| CAAGAAACAGAAGAAGGTTT+CGG | 0.449162 | 7.1:+7484178 | MS.gene36536:CDS |
| ATCTGCTAAGAAATTGTTTG+AGG | 0.450418 | 7.1:+7484022 | MS.gene36536:CDS |
| CTTCCCTCTCGAATTTCTGC+GGG | 0.460361 | 7.1:-7484460 | None:intergenic |
| TTCATCTTTCTTTCTAAGCA+AGG | 0.478456 | 7.1:-7484341 | None:intergenic |
| AATGGAGTGGCAATTGTTGA+AGG | 0.525932 | 7.1:+7483968 | None:intergenic |
| CTCTTTAACAATCCTTTCAA+CGG | 0.526642 | 7.1:-7484131 | None:intergenic |
| GGTTGCTCAGATTAGGGTGA+TGG | 0.528944 | 7.1:+7484223 | MS.gene36536:CDS |
| AGAGGGAAGAAGCTGGTGCT+AGG | 0.536510 | 7.1:+7484474 | MS.gene36536:CDS |
| TCTTCCCTCTCGAATTTCTG+CGG | 0.553717 | 7.1:-7484461 | None:intergenic |
| CAATCTGATGAACCGTTGAA+AGG | 0.557133 | 7.1:+7484119 | MS.gene36536:CDS |
| ATAATACCCTGACACAGATA+AGG | 0.564227 | 7.1:-7484313 | None:intergenic |
| AGATAAGGCCTAAAATCTCT+CGG | 0.585108 | 7.1:-7484298 | None:intergenic |
| TTTAGGCCTTATCTGTGTCA+GGG | 0.591449 | 7.1:+7484307 | MS.gene36536:CDS |
| AGCATTTCATGGACTTGTGA+TGG | 0.594852 | 7.1:+7484076 | MS.gene36536:CDS |
| GTTGTGCAAGAAACAGAAGA+AGG | 0.595826 | 7.1:+7484172 | MS.gene36536:CDS |
| AAATTCGAGAGGGAAGAAGC+TGG | 0.597290 | 7.1:+7484467 | MS.gene36536:CDS |
| CTTAGAAAGAAAGATGAAAG+CGG | 0.601160 | 7.1:+7484346 | MS.gene36536:CDS |
| GCTCAGATTAGGGTGATGGA+AGG | 0.608499 | 7.1:+7484227 | MS.gene36536:CDS |
| CTCAGATTAGGGTGATGGAA+GGG | 0.632777 | 7.1:+7484228 | MS.gene36536:CDS |
| AATTGAACTAAGAAATGGAG+TGG | 0.636484 | 7.1:+7483955 | None:intergenic |
| TGAAATGCTTCAACTCGAAG+CGG | 0.637606 | 7.1:-7484062 | None:intergenic |
| TTTCCCGCAGAAATTCGAGA+GGG | 0.688033 | 7.1:+7484457 | MS.gene36536:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCATCAAAATACTCTTTATA+AGG | - | chr7.1:7484416-7484435 | None:intergenic | 20.0% |
| !!! | TATTTTGATGATAACATGAA+TGG | + | chr7.1:7484425-7484444 | MS.gene36536:CDS | 20.0% |
| ! | TCTTTATAAGGATGATTCTT+CGG | - | chr7.1:7484404-7484423 | None:intergenic | 25.0% |
| AAAGAGAGTTGAAAAAGCAA+TGG | + | chr7.1:7484148-7484167 | MS.gene36536:CDS | 30.0% | |
| ACAATTTCTTAGCAGATTCA+TGG | - | chr7.1:7484019-7484038 | None:intergenic | 30.0% | |
| ATCTGCTAAGAAATTGTTTG+AGG | + | chr7.1:7484022-7484041 | MS.gene36536:CDS | 30.0% | |
| CTCTTTAACAATCCTTTCAA+CGG | - | chr7.1:7484134-7484153 | None:intergenic | 30.0% | |
| CTTAGAAAGAAAGATGAAAG+CGG | + | chr7.1:7484346-7484365 | MS.gene36536:CDS | 30.0% | |
| CTTTATAAGGATGATTCTTC+GGG | - | chr7.1:7484403-7484422 | None:intergenic | 30.0% | |
| TTCATCTTTCTTTCTAAGCA+AGG | - | chr7.1:7484344-7484363 | None:intergenic | 30.0% | |
| !!! | TGTTAGGGATTTTAGGTTAT+TGG | + | chr7.1:7484202-7484221 | MS.gene36536:CDS | 30.0% |
| AGATAAGGCCTAAAATCTCT+CGG | - | chr7.1:7484301-7484320 | None:intergenic | 35.0% | |
| ATAATACCCTGACACAGATA+AGG | - | chr7.1:7484316-7484335 | None:intergenic | 35.0% | |
| CAAGAAACAGAAGAAGGTTT+CGG | + | chr7.1:7484178-7484197 | MS.gene36536:CDS | 35.0% | |
| GCATAAAGCTTATCAGGATT+TGG | + | chr7.1:7484265-7484284 | MS.gene36536:CDS | 35.0% | |
| ! | AAGAAGAACCGAGAGATTTT+AGG | + | chr7.1:7484290-7484309 | MS.gene36536:CDS | 35.0% |
| ! | GTTATTGGTTGCTCAGATTA+GGG | + | chr7.1:7484217-7484236 | MS.gene36536:CDS | 35.0% |
| ! | TTTCGGATGTTAGGGATTTT+AGG | + | chr7.1:7484195-7484214 | MS.gene36536:CDS | 35.0% |
| AGAAGAAGGTTTCGGATGTT+AGG | + | chr7.1:7484186-7484205 | MS.gene36536:CDS | 40.0% | |
| AGCATTTCATGGACTTGTGA+TGG | + | chr7.1:7484076-7484095 | MS.gene36536:CDS | 40.0% | |
| CAATCTGATGAACCGTTGAA+AGG | + | chr7.1:7484119-7484138 | MS.gene36536:CDS | 40.0% | |
| GAAGAAGGTTTCGGATGTTA+GGG | + | chr7.1:7484187-7484206 | MS.gene36536:CDS | 40.0% | |
| GTTGTGCAAGAAACAGAAGA+AGG | + | chr7.1:7484172-7484191 | MS.gene36536:CDS | 40.0% | |
| TGAAATGCTTCAACTCGAAG+CGG | - | chr7.1:7484065-7484084 | None:intergenic | 40.0% | |
| ! | GGTTATTGGTTGCTCAGATT+AGG | + | chr7.1:7484216-7484235 | MS.gene36536:CDS | 40.0% |
| ! | TTTAGGCCTTATCTGTGTCA+GGG | + | chr7.1:7484307-7484326 | MS.gene36536:CDS | 40.0% |
| !! | CTTCGAGTTGAAGCATTTCA+TGG | + | chr7.1:7484065-7484084 | MS.gene36536:CDS | 40.0% |
| !! | GATGGAAGGGAATTTTTCAG+AGG | + | chr7.1:7484241-7484260 | MS.gene36536:CDS | 40.0% |
| !! | TTTTAGGCCTTATCTGTGTC+AGG | + | chr7.1:7484306-7484325 | MS.gene36536:CDS | 40.0% |
| AAATTCGAGAGGGAAGAAGC+TGG | + | chr7.1:7484467-7484486 | MS.gene36536:CDS | 45.0% | |
| TCTTCCCTCTCGAATTTCTG+CGG | - | chr7.1:7484464-7484483 | None:intergenic | 45.0% | |
| TTTCCCGCAGAAATTCGAGA+GGG | + | chr7.1:7484457-7484476 | MS.gene36536:CDS | 45.0% | |
| ! | AGAGGCGCATAAAGCTTATC+AGG | + | chr7.1:7484259-7484278 | MS.gene36536:CDS | 45.0% |
| ! | TTTTCCCGCAGAAATTCGAG+AGG | + | chr7.1:7484456-7484475 | MS.gene36536:CDS | 45.0% |
| !! | CTCAGATTAGGGTGATGGAA+GGG | + | chr7.1:7484228-7484247 | MS.gene36536:CDS | 45.0% |
| CTTCCCTCTCGAATTTCTGC+GGG | - | chr7.1:7484463-7484482 | None:intergenic | 50.0% | |
| GGTTGCTCAGATTAGGGTGA+TGG | + | chr7.1:7484223-7484242 | MS.gene36536:CDS | 50.0% | |
| ! | TGGACTTGTGATGGCGAGTT+CGG | + | chr7.1:7484085-7484104 | MS.gene36536:CDS | 50.0% |
| !! | GCTCAGATTAGGGTGATGGA+AGG | + | chr7.1:7484227-7484246 | MS.gene36536:CDS | 50.0% |
| AGAGGGAAGAAGCTGGTGCT+AGG | + | chr7.1:7484474-7484493 | MS.gene36536:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 7483969 | 7484526 | 7483969 | ID=MS.gene36536 |
| chr7.1 | mRNA | 7483969 | 7484526 | 7483969 | ID=MS.gene36536.t1;Parent=MS.gene36536 |
| chr7.1 | exon | 7483969 | 7484526 | 7483969 | ID=MS.gene36536.t1.exon1;Parent=MS.gene36536.t1 |
| chr7.1 | CDS | 7483969 | 7484526 | 7483969 | ID=cds.MS.gene36536.t1;Parent=MS.gene36536.t1 |
| Gene Sequence |
| Protein sequence |
