Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36869.t1 | XP_013444919.1 | 91.4 | 187 | 14 | 2 | 1 | 187 | 1 | 185 | 7.10E-89 | 336.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36869.t1 | A0A072TN82 | 91.4 | 187 | 14 | 2 | 1 | 187 | 1 | 185 | 5.1e-89 | 336.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057071 | MS.gene36869 | 0.807005 | 6.24E-50 | -1.69E-46 |
| MS.gene057208 | MS.gene36869 | 0.825795 | 3.86E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36869.t1 | MTR_8g432480 | 91.444 | 187 | 14 | 2 | 1 | 187 | 1 | 185 | 1.44e-121 | 341 |
| MS.gene36869.t1 | MTR_8g432460 | 54.802 | 177 | 60 | 4 | 7 | 169 | 5 | 175 | 9.75e-54 | 169 |
| MS.gene36869.t1 | MTR_8g432440 | 54.237 | 177 | 60 | 5 | 7 | 168 | 5 | 175 | 4.71e-52 | 165 |
| MS.gene36869.t1 | MTR_8g432420 | 54.237 | 177 | 60 | 5 | 7 | 168 | 5 | 175 | 4.71e-52 | 165 |
| MS.gene36869.t1 | MTR_8g432490 | 52.841 | 176 | 63 | 4 | 7 | 168 | 5 | 174 | 2.85e-51 | 163 |
| MS.gene36869.t1 | MTR_8g432470 | 51.412 | 177 | 65 | 4 | 7 | 168 | 5 | 175 | 8.88e-50 | 159 |
| MS.gene36869.t1 | MTR_8g432450 | 51.685 | 178 | 64 | 5 | 7 | 168 | 5 | 176 | 4.98e-49 | 157 |
| MS.gene36869.t1 | MTR_3g064780 | 50.282 | 177 | 68 | 4 | 7 | 169 | 5 | 175 | 3.26e-48 | 157 |
| MS.gene36869.t1 | MTR_8g432370 | 34.884 | 129 | 77 | 4 | 7 | 131 | 2 | 127 | 6.57e-18 | 77.4 |
| MS.gene36869.t1 | MTR_8g432400 | 35.156 | 128 | 79 | 3 | 7 | 131 | 2 | 128 | 8.47e-17 | 74.7 |
| MS.gene36869.t1 | MTR_8g432390 | 35.385 | 130 | 76 | 5 | 7 | 131 | 2 | 128 | 2.80e-16 | 73.2 |
| MS.gene36869.t1 | MTR_8g432350 | 34.615 | 130 | 77 | 5 | 7 | 131 | 2 | 128 | 2.95e-16 | 73.2 |
| MS.gene36869.t1 | MTR_7g076000 | 33.918 | 171 | 91 | 8 | 7 | 169 | 2 | 158 | 4.72e-14 | 67.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 33 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTCATTGGGATTCCAGTTT+TGG | 0.209973 | 8.4:+64124907 | MS.gene36869:CDS |
| TTAGAAATCACCATTATTTC+CGG | 0.251148 | 8.4:+64124446 | MS.gene36869:CDS |
| AAGCGTTGATCGAAATTTAA+AGG | 0.259589 | 8.4:+64124478 | MS.gene36869:CDS |
| AAAACGACGGTTTACTTTCA+TGG | 0.293418 | 8.4:+64124567 | MS.gene36869:CDS |
| GAGTTATGGGTTGAGGGATT+GGG | 0.319102 | 8.4:+64124751 | MS.gene36869:CDS |
| TGAGTTATGGGTTGAGGGAT+TGG | 0.329761 | 8.4:+64124750 | MS.gene36869:CDS |
| AATTCAAATCATGTTGTCAT+TGG | 0.330631 | 8.4:+64124893 | MS.gene36869:CDS |
| GTGTTCAAACTTTGAGTTAT+GGG | 0.372826 | 8.4:+64124738 | MS.gene36869:CDS |
| ATTCAAATCATGTTGTCATT+GGG | 0.399832 | 8.4:+64124894 | MS.gene36869:CDS |
| AACTTTGAGTTATGGGTTGA+GGG | 0.424777 | 8.4:+64124745 | MS.gene36869:CDS |
| AAACTTTGAGTTATGGGTTG+AGG | 0.441165 | 8.4:+64124744 | MS.gene36869:CDS |
| GGTGTTCAAACTTTGAGTTA+TGG | 0.451609 | 8.4:+64124737 | MS.gene36869:CDS |
| TGTTGAGGATGAGGGGTTGA+AGG | 0.474497 | 8.4:+64124859 | MS.gene36869:CDS |
| TGCAAAATCAGTGACGTTTG+AGG | 0.496537 | 8.4:+64124622 | MS.gene36869:CDS |
| AGAACAACGTGTTGAGGATG+AGG | 0.497164 | 8.4:+64124850 | MS.gene36869:CDS |
| TGATCGAAATTTAAAGGCAG+AGG | 0.511979 | 8.4:+64124484 | MS.gene36869:CDS |
| GTGATTTCTAATGTGTGTGC+TGG | 0.514168 | 8.4:-64124434 | None:intergenic |
| TACAAAGGAGCTCGTCCCAT+TGG | 0.515512 | 8.4:+64124659 | MS.gene36869:CDS |
| GGTTGAGGGATTGGGATGGT+AGG | 0.532351 | 8.4:+64124759 | MS.gene36869:CDS |
| TGAGGATGAGGGGTTGAAGG+TGG | 0.539423 | 8.4:+64124862 | MS.gene36869:CDS |
| GCTATCCTAGCCACTCCAAT+GGG | 0.544573 | 8.4:-64124674 | None:intergenic |
| TGCTATCCTAGCCACTCCAA+TGG | 0.578717 | 8.4:-64124675 | None:intergenic |
| AGTGGCTAGGATAGCACTAT+CGG | 0.591367 | 8.4:+64124682 | MS.gene36869:CDS |
| GAACAACGTGTTGAGGATGA+GGG | 0.595440 | 8.4:+64124851 | MS.gene36869:CDS |
| TATGGGTTGAGGGATTGGGA+TGG | 0.614237 | 8.4:+64124755 | MS.gene36869:CDS |
| TATTTATGTTGTTGTTCGTG+CGG | 0.619888 | 8.4:+64124508 | MS.gene36869:CDS |
| AGAAGGGTGGTGACACAAGA+AGG | 0.628535 | 8.4:+64124809 | MS.gene36869:CDS |
| CTCGTCCCATTGGAGTGGCT+AGG | 0.651174 | 8.4:+64124669 | MS.gene36869:CDS |
| GTACAATGCAAGAAGTACAA+AGG | 0.653282 | 8.4:+64124644 | MS.gene36869:CDS |
| AGGAGCTCGTCCCATTGGAG+TGG | 0.656408 | 8.4:+64124664 | MS.gene36869:CDS |
| AACAACGTGTTGAGGATGAG+GGG | 0.664340 | 8.4:+64124852 | MS.gene36869:CDS |
| GACGATTCGTCGTTGCAAAG+TGG | 0.704449 | 8.4:+64124716 | MS.gene36869:CDS |
| AAAATGGTGAAAGAAAACGA+CGG | 0.784435 | 8.4:+64124554 | MS.gene36869:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATTAATTTTTGTGTGAGAA+GGG | + | chr8.4:64124793-64124812 | MS.gene36869:CDS | 20.0% |
| !!! | TTATTAATTTTTGTGTGAGA+AGG | + | chr8.4:64124792-64124811 | MS.gene36869:CDS | 20.0% |
| ! | AATTCAAATCATGTTGTCAT+TGG | + | chr8.4:64124893-64124912 | MS.gene36869:CDS | 25.0% |
| ! | ATTCAAATCATGTTGTCATT+GGG | + | chr8.4:64124894-64124913 | MS.gene36869:CDS | 25.0% |
| ! | TTAGAAATCACCATTATTTC+CGG | + | chr8.4:64124446-64124465 | MS.gene36869:CDS | 25.0% |
| AAAATGGTGAAAGAAAACGA+CGG | + | chr8.4:64124554-64124573 | MS.gene36869:CDS | 30.0% | |
| AGAAACAAAAAACCCAAAAC+TGG | - | chr8.4:64124923-64124942 | None:intergenic | 30.0% | |
| ! | GTGTTCAAACTTTGAGTTAT+GGG | + | chr8.4:64124738-64124757 | MS.gene36869:CDS | 30.0% |
| ! | TATGTTTTGTCCGGAAATAA+TGG | - | chr8.4:64124459-64124478 | None:intergenic | 30.0% |
| ! | TATTTATGTTGTTGTTCGTG+CGG | + | chr8.4:64124508-64124527 | MS.gene36869:CDS | 30.0% |
| !! | AAGCGTTGATCGAAATTTAA+AGG | + | chr8.4:64124478-64124497 | MS.gene36869:CDS | 30.0% |
| !!! | TTGGGTTTTTTGTTTCTCTT+GGG | + | chr8.4:64124926-64124945 | MS.gene36869:CDS | 30.0% |
| !!! | TTTGGGTTTTTTGTTTCTCT+TGG | + | chr8.4:64124925-64124944 | MS.gene36869:CDS | 30.0% |
| AAAACGACGGTTTACTTTCA+TGG | + | chr8.4:64124567-64124586 | MS.gene36869:CDS | 35.0% | |
| AGAAAAAGAACAACGTGTTG+AGG | + | chr8.4:64124844-64124863 | MS.gene36869:CDS | 35.0% | |
| CAACTCTTGCAAGACAAAAA+TGG | + | chr8.4:64124538-64124557 | MS.gene36869:CDS | 35.0% | |
| TGATCGAAATTTAAAGGCAG+AGG | + | chr8.4:64124484-64124503 | MS.gene36869:CDS | 35.0% | |
| ! | AAACTTTGAGTTATGGGTTG+AGG | + | chr8.4:64124744-64124763 | MS.gene36869:CDS | 35.0% |
| ! | AACTTTGAGTTATGGGTTGA+GGG | + | chr8.4:64124745-64124764 | MS.gene36869:CDS | 35.0% |
| ! | ATAGCACTATCGGATTTTCT+TGG | + | chr8.4:64124692-64124711 | MS.gene36869:CDS | 35.0% |
| ! | ATCAACGCTTATGTTTTGTC+CGG | - | chr8.4:64124468-64124487 | None:intergenic | 35.0% |
| ! | GGTGTTCAAACTTTGAGTTA+TGG | + | chr8.4:64124737-64124756 | MS.gene36869:CDS | 35.0% |
| ! | GTACAATGCAAGAAGTACAA+AGG | + | chr8.4:64124644-64124663 | MS.gene36869:CDS | 35.0% |
| ! | TAATTTTTGTGTGAGAAGGG+TGG | + | chr8.4:64124796-64124815 | MS.gene36869:CDS | 35.0% |
| GATTGGGATGGTAGGAAAAA+TGG | + | chr8.4:64124767-64124786 | MS.gene36869:CDS | 40.0% | |
| GTGATTTCTAATGTGTGTGC+TGG | - | chr8.4:64124437-64124456 | None:intergenic | 40.0% | |
| TGCAAAATCAGTGACGTTTG+AGG | + | chr8.4:64124622-64124641 | MS.gene36869:CDS | 40.0% | |
| ! | GTCATTGGGATTCCAGTTTT+GGG | + | chr8.4:64124908-64124927 | MS.gene36869:CDS | 40.0% |
| ! | TGTCATTGGGATTCCAGTTT+TGG | + | chr8.4:64124907-64124926 | MS.gene36869:CDS | 40.0% |
| !!! | AACGTCACTGATTTTGCATG+TGG | - | chr8.4:64124620-64124639 | None:intergenic | 40.0% |
| AACAACGTGTTGAGGATGAG+GGG | + | chr8.4:64124852-64124871 | MS.gene36869:CDS | 45.0% | |
| AGAACAACGTGTTGAGGATG+AGG | + | chr8.4:64124850-64124869 | MS.gene36869:CDS | 45.0% | |
| AGTGGCTAGGATAGCACTAT+CGG | + | chr8.4:64124682-64124701 | MS.gene36869:CDS | 45.0% | |
| GAACAACGTGTTGAGGATGA+GGG | + | chr8.4:64124851-64124870 | MS.gene36869:CDS | 45.0% | |
| ! | GAGTTATGGGTTGAGGGATT+GGG | + | chr8.4:64124751-64124770 | MS.gene36869:CDS | 45.0% |
| ! | TGAGTTATGGGTTGAGGGAT+TGG | + | chr8.4:64124750-64124769 | MS.gene36869:CDS | 45.0% |
| AGAAGGGTGGTGACACAAGA+AGG | + | chr8.4:64124809-64124828 | MS.gene36869:CDS | 50.0% | |
| GACGATTCGTCGTTGCAAAG+TGG | + | chr8.4:64124716-64124735 | MS.gene36869:CDS | 50.0% | |
| GCTATCCTAGCCACTCCAAT+GGG | - | chr8.4:64124677-64124696 | None:intergenic | 50.0% | |
| TACAAAGGAGCTCGTCCCAT+TGG | + | chr8.4:64124659-64124678 | MS.gene36869:CDS | 50.0% | |
| TGCTATCCTAGCCACTCCAA+TGG | - | chr8.4:64124678-64124697 | None:intergenic | 50.0% | |
| TGTTGAGGATGAGGGGTTGA+AGG | + | chr8.4:64124859-64124878 | MS.gene36869:CDS | 50.0% | |
| ! | TATGGGTTGAGGGATTGGGA+TGG | + | chr8.4:64124755-64124774 | MS.gene36869:CDS | 50.0% |
| GGTTGAGGGATTGGGATGGT+AGG | + | chr8.4:64124759-64124778 | MS.gene36869:CDS | 55.0% | |
| TGAGGATGAGGGGTTGAAGG+TGG | + | chr8.4:64124862-64124881 | MS.gene36869:CDS | 55.0% | |
| AGGAGCTCGTCCCATTGGAG+TGG | + | chr8.4:64124664-64124683 | MS.gene36869:CDS | 60.0% | |
| CTCGTCCCATTGGAGTGGCT+AGG | + | chr8.4:64124669-64124688 | MS.gene36869:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 64124419 | 64124982 | 64124419 | ID=MS.gene36869 |
| chr8.4 | mRNA | 64124419 | 64124982 | 64124419 | ID=MS.gene36869.t1;Parent=MS.gene36869 |
| chr8.4 | exon | 64124419 | 64124982 | 64124419 | ID=MS.gene36869.t1.exon1;Parent=MS.gene36869.t1 |
| chr8.4 | CDS | 64124419 | 64124982 | 64124419 | ID=cds.MS.gene36869.t1;Parent=MS.gene36869.t1 |
| Gene Sequence |
| Protein sequence |
