Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37088.t1 | KEH20775.1 | 92.5 | 107 | 8 | 0 | 1 | 107 | 1 | 107 | 5.40E-49 | 203.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37088.t1 | A0A072U4M2 | 92.5 | 107 | 8 | 0 | 1 | 107 | 1 | 107 | 3.9e-49 | 203.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052340 | MS.gene37088 | 0.806224 | 9.13E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37088.t1 | MTR_8g089830 | 92.523 | 107 | 8 | 0 | 1 | 107 | 1 | 107 | 8.33e-72 | 209 |
| MS.gene37088.t1 | MTR_8g089825 | 76.190 | 105 | 20 | 4 | 1 | 103 | 1 | 102 | 2.61e-50 | 154 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTGTTGTGGTTTCGGTTT+TGG | 0.230297 | 4.3:-10574318 | None:intergenic |
| ACCCGTAATTGTTGTGGTTT+CGG | 0.263916 | 4.3:-10574324 | None:intergenic |
| AACCGAAACCACAACAATTA+CGG | 0.350207 | 4.3:+10574322 | MS.gene37088:CDS |
| GATTGATCTTCCTCAACTAT+TGG | 0.360870 | 4.3:-10574430 | None:intergenic |
| CCTCATTGATGACATGAGTT+TGG | 0.382010 | 4.3:-10574602 | None:intergenic |
| ACCGAAACCACAACAATTAC+GGG | 0.390353 | 4.3:+10574323 | MS.gene37088:CDS |
| AGATCAATCCTACTATTTAG+AGG | 0.394992 | 4.3:+10574444 | MS.gene37088:CDS |
| TTACGAACCCGCATACTATC+CGG | 0.409986 | 4.3:+10574366 | MS.gene37088:CDS |
| TACGAACCCGCATACTATCC+GGG | 0.436849 | 4.3:+10574367 | MS.gene37088:CDS |
| ACGACGAACCTCTAAATAGT+AGG | 0.482372 | 4.3:-10574452 | None:intergenic |
| AGACCGATCGACGAGGTTTG+AGG | 0.483942 | 4.3:+10574488 | MS.gene37088:CDS |
| GATCGACGAGGTTTGAGGTA+TGG | 0.517220 | 4.3:+10574493 | MS.gene37088:CDS |
| GCGTCGTGTTGCAATATATG+AGG | 0.518107 | 4.3:+10574405 | MS.gene37088:CDS |
| ATACCTCAAACCTCGTCGAT+CGG | 0.542925 | 4.3:-10574491 | None:intergenic |
| TTATTCAACACGAGCATAGG+AGG | 0.555341 | 4.3:+10574569 | MS.gene37088:CDS |
| CAAACTCATGTCATCAATGA+GGG | 0.571315 | 4.3:+10574603 | MS.gene37088:CDS |
| GACTCCACCCGTAATTGTTG+TGG | 0.580902 | 4.3:-10574330 | None:intergenic |
| GTGATGCCCCGGATAGTATG+CGG | 0.591042 | 4.3:-10574374 | None:intergenic |
| CCAAACTCATGTCATCAATG+AGG | 0.606593 | 4.3:+10574602 | MS.gene37088:CDS |
| TGATGCCCCGGATAGTATGC+GGG | 0.606955 | 4.3:-10574373 | None:intergenic |
| TCAACACGAGCATAGGAGGA+TGG | 0.624383 | 4.3:+10574573 | MS.gene37088:CDS |
| ATATGAGGAACCAATAGTTG+AGG | 0.635820 | 4.3:+10574420 | MS.gene37088:CDS |
| GAAACCACAACAATTACGGG+TGG | 0.673197 | 4.3:+10574326 | MS.gene37088:CDS |
| ACGAACCCGCATACTATCCG+GGG | 0.673282 | 4.3:+10574368 | MS.gene37088:CDS |
| GAGACTGAGACCGATCGACG+AGG | 0.700755 | 4.3:+10574481 | MS.gene37088:CDS |
| AAGTGTTGATGAAGAAGCTG+AGG | 0.703686 | 4.3:+10574543 | MS.gene37088:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGATCAATCCTACTATTTAG+AGG | + | chr4.3:10574444-10574463 | MS.gene37088:CDS | 30.0% | |
| AACCGAAACCACAACAATTA+CGG | + | chr4.3:10574322-10574341 | MS.gene37088:CDS | 35.0% | |
| ATATGAGGAACCAATAGTTG+AGG | + | chr4.3:10574420-10574439 | MS.gene37088:CDS | 35.0% | |
| CAAACTCATGTCATCAATGA+GGG | + | chr4.3:10574603-10574622 | MS.gene37088:CDS | 35.0% | |
| GATTGATCTTCCTCAACTAT+TGG | - | chr4.3:10574433-10574452 | None:intergenic | 35.0% | |
| ! | AATTGTTGTGGTTTCGGTTT+TGG | - | chr4.3:10574321-10574340 | None:intergenic | 35.0% |
| ! | CTTTTATTCAACACGAGCAT+AGG | + | chr4.3:10574566-10574585 | MS.gene37088:CDS | 35.0% |
| ACCCGTAATTGTTGTGGTTT+CGG | - | chr4.3:10574327-10574346 | None:intergenic | 40.0% | |
| ACCGAAACCACAACAATTAC+GGG | + | chr4.3:10574323-10574342 | MS.gene37088:CDS | 40.0% | |
| ACGACGAACCTCTAAATAGT+AGG | - | chr4.3:10574455-10574474 | None:intergenic | 40.0% | |
| CCAAACTCATGTCATCAATG+AGG | + | chr4.3:10574602-10574621 | MS.gene37088:CDS | 40.0% | |
| TTATTCAACACGAGCATAGG+AGG | + | chr4.3:10574569-10574588 | MS.gene37088:CDS | 40.0% | |
| ! | CCTCATTGATGACATGAGTT+TGG | - | chr4.3:10574605-10574624 | None:intergenic | 40.0% |
| !! | AAGTGTTGATGAAGAAGCTG+AGG | + | chr4.3:10574543-10574562 | MS.gene37088:CDS | 40.0% |
| ATACCTCAAACCTCGTCGAT+CGG | - | chr4.3:10574494-10574513 | None:intergenic | 45.0% | |
| GAAACCACAACAATTACGGG+TGG | + | chr4.3:10574326-10574345 | MS.gene37088:CDS | 45.0% | |
| GCGTCGTGTTGCAATATATG+AGG | + | chr4.3:10574405-10574424 | MS.gene37088:CDS | 45.0% | |
| TTACGAACCCGCATACTATC+CGG | + | chr4.3:10574366-10574385 | MS.gene37088:CDS | 45.0% | |
| ! | TTGCAACACGACGCTTTTGA+TGG | - | chr4.3:10574399-10574418 | None:intergenic | 45.0% |
| GACTCCACCCGTAATTGTTG+TGG | - | chr4.3:10574333-10574352 | None:intergenic | 50.0% | |
| TACGAACCCGCATACTATCC+GGG | + | chr4.3:10574367-10574386 | MS.gene37088:CDS | 50.0% | |
| TCAACACGAGCATAGGAGGA+TGG | + | chr4.3:10574573-10574592 | MS.gene37088:CDS | 50.0% | |
| ! | GATCGACGAGGTTTGAGGTA+TGG | + | chr4.3:10574493-10574512 | MS.gene37088:CDS | 50.0% |
| ACGAACCCGCATACTATCCG+GGG | + | chr4.3:10574368-10574387 | MS.gene37088:CDS | 55.0% | |
| AGACCGATCGACGAGGTTTG+AGG | + | chr4.3:10574488-10574507 | MS.gene37088:CDS | 55.0% | |
| GTGATGCCCCGGATAGTATG+CGG | - | chr4.3:10574377-10574396 | None:intergenic | 55.0% | |
| TGATGCCCCGGATAGTATGC+GGG | - | chr4.3:10574376-10574395 | None:intergenic | 55.0% | |
| !!! | CGCTTTTGATGGTGATGCCC+CGG | - | chr4.3:10574388-10574407 | None:intergenic | 55.0% |
| GAGACTGAGACCGATCGACG+AGG | + | chr4.3:10574481-10574500 | MS.gene37088:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 10574310 | 10574633 | 10574310 | ID=MS.gene37088 |
| chr4.3 | mRNA | 10574310 | 10574633 | 10574310 | ID=MS.gene37088.t1;Parent=MS.gene37088 |
| chr4.3 | exon | 10574310 | 10574633 | 10574310 | ID=MS.gene37088.t1.exon1;Parent=MS.gene37088.t1 |
| chr4.3 | CDS | 10574310 | 10574633 | 10574310 | ID=cds.MS.gene37088.t1;Parent=MS.gene37088.t1 |
| Gene Sequence |
| Protein sequence |
