Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37572.t1 | XP_003617202.1 | 95.8 | 240 | 9 | 1 | 1 | 239 | 1 | 240 | 1.30E-114 | 422.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37572.t1 | G7KCU0 | 95.8 | 240 | 9 | 1 | 1 | 239 | 1 | 240 | 9.1e-115 | 422.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056092 | MS.gene37572 | 0.802076 | 6.66E-49 | -1.69E-46 |
| MS.gene056752 | MS.gene37572 | 0.837707 | 4.43E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37572.t1 | MTR_5g088990 | 95.417 | 240 | 10 | 1 | 1 | 239 | 1 | 240 | 3.20e-169 | 466 |
| MS.gene37572.t1 | MTR_7g075770 | 40.940 | 149 | 78 | 5 | 9 | 155 | 8 | 148 | 6.28e-26 | 100 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37572.t1 | AT1G08500 | 59.278 | 194 | 73 | 3 | 13 | 200 | 7 | 200 | 6.62e-78 | 234 |
Find 42 sgRNAs with CRISPR-Local
Find 98 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCCAAGCTGAATTCTTTCT+TGG | 0.237475 | 5.4:-70086743 | None:intergenic |
| AAATTTCACAACATGTTATA+TGG | 0.242967 | 5.4:+70088281 | MS.gene37572:CDS |
| TCTCCGGCTAGTCCGATTTC+TGG | 0.249790 | 5.4:+70088122 | MS.gene37572:CDS |
| TTCCTTAGGATGGTTGAAAT+TGG | 0.295594 | 5.4:-70088180 | None:intergenic |
| CAACTGTTAATTATCAGAAA+TGG | 0.300214 | 5.4:+70086716 | MS.gene37572:CDS |
| TCATCGTCACTGTCTTCCTT+AGG | 0.390911 | 5.4:-70088194 | None:intergenic |
| TCATGTTTCTTAGGTTCTGT+TGG | 0.394187 | 5.4:+70086634 | MS.gene37572:CDS |
| TACACAGTGGGAGATTCTTT+AGG | 0.400880 | 5.4:+70086670 | MS.gene37572:CDS |
| CTTTAACATCGAAACAGAAC+TGG | 0.404508 | 5.4:-70088252 | None:intergenic |
| AACTGTTAATTATCAGAAAT+GGG | 0.420262 | 5.4:+70086717 | MS.gene37572:CDS |
| AAAGGAACTGCCACTGTGAC+TGG | 0.433267 | 5.4:-70087960 | None:intergenic |
| GTTGCCCCTTCTTTCAGTAA+AGG | 0.467797 | 5.4:-70087978 | None:intergenic |
| GACTCATCGTCACCAGAAAT+CGG | 0.470529 | 5.4:-70088134 | None:intergenic |
| GCAGTTCCTTTACTGAAAGA+AGG | 0.475413 | 5.4:+70087972 | MS.gene37572:CDS |
| TGACAAAGATAAAGATAAAG+AGG | 0.482717 | 5.4:+70088226 | MS.gene37572:CDS |
| GCCAAGAAAGAATTCAGCTT+GGG | 0.483597 | 5.4:+70086742 | MS.gene37572:CDS |
| TTGGAAGGAATATTTGTGTC+TGG | 0.492247 | 5.4:-70088161 | None:intergenic |
| TTCTTCTCTAGTGATTATGA+TGG | 0.501250 | 5.4:+70088005 | MS.gene37572:CDS |
| GTCGGAAGATTCTCCATCTC+CGG | 0.504591 | 5.4:+70088106 | MS.gene37572:CDS |
| CAGTGGGAGATTCTTTAGGC+TGG | 0.508955 | 5.4:+70086674 | MS.gene37572:CDS |
| ACGGATCAACTGATGACCAT+TGG | 0.511597 | 5.4:-70087920 | None:intergenic |
| CGTCACTGTCTTCCTTAGGA+TGG | 0.511800 | 5.4:-70088190 | None:intergenic |
| TTAGGATGGTTGAAATTGGA+AGG | 0.515170 | 5.4:-70088176 | None:intergenic |
| CATTTCTGATAATTAACAGT+TGG | 0.517247 | 5.4:-70086715 | None:intergenic |
| CAGTTCCTTTACTGAAAGAA+GGG | 0.524215 | 5.4:+70087973 | MS.gene37572:CDS |
| TTCAAAATAAATGTGACTCA+TGG | 0.525741 | 5.4:+70088053 | MS.gene37572:CDS |
| TTCCAATTTCAACCATCCTA+AGG | 0.526093 | 5.4:+70088178 | MS.gene37572:CDS |
| TATATGTTTGAACAAGAGAG+TGG | 0.543824 | 5.4:-70087842 | None:intergenic |
| TCACCAGAAATCGGACTAGC+CGG | 0.553642 | 5.4:-70088125 | None:intergenic |
| ATAATCACATTGTTTGTATG+TGG | 0.560805 | 5.4:-70087871 | None:intergenic |
| TGCCAAGAAAGAATTCAGCT+TGG | 0.561777 | 5.4:+70086741 | MS.gene37572:CDS |
| GAAATCGGACTAGCCGGAGA+TGG | 0.573901 | 5.4:-70088119 | None:intergenic |
| GACTGGATGTATATCAGTGT+TGG | 0.601352 | 5.4:-70087943 | None:intergenic |
| ATAAATGTGACTCATGGACA+AGG | 0.609850 | 5.4:+70088059 | MS.gene37572:CDS |
| TTATGATGATGCACAAGACA+AGG | 0.629304 | 5.4:+70087890 | MS.gene37572:CDS |
| AGTTCCTTTACTGAAAGAAG+GGG | 0.634152 | 5.4:+70087974 | MS.gene37572:CDS |
| GGATGTATATCAGTGTTGGA+CGG | 0.669469 | 5.4:-70087939 | None:intergenic |
| GGAGATGGAGAATCTTCCGA+CGG | 0.671736 | 5.4:-70088104 | None:intergenic |
| TAAATGTGACTCATGGACAA+GGG | 0.675226 | 5.4:+70088060 | MS.gene37572:CDS |
| AAGATGTCCCTAATAAAACA+AGG | 0.680872 | 5.4:-70088307 | None:intergenic |
| AAGACAAGGACACAATCCAA+TGG | 0.682396 | 5.4:+70087904 | MS.gene37572:CDS |
| TGATATACATCCAGTCACAG+TGG | 0.752267 | 5.4:+70087950 | MS.gene37572:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGAAAGGAATATTAAAAAA+AGG | - | chr5.4:70087579-70087598 | None:intergenic | 15.0% |
| !! | TATAAAAGTTATGCTTAAAT+TGG | + | chr5.4:70087340-70087359 | MS.gene37572:intron | 15.0% |
| !!! | TTTTTAATATTCCTTTCTTT+TGG | + | chr5.4:70087580-70087599 | MS.gene37572:intron | 15.0% |
| !! | AAAAATAAAATCACTTACTG+AGG | - | chr5.4:70086774-70086793 | None:intergenic | 20.0% |
| !! | AAAGTGGTATAAAATTAGAT+TGG | + | chr5.4:70087206-70087225 | MS.gene37572:intron | 20.0% |
| !! | AAATAAAAACATAACCACTT+AGG | - | chr5.4:70087286-70087305 | None:intergenic | 20.0% |
| !! | AAATTAACAAACCAAACTTT+AGG | - | chr5.4:70087641-70087660 | None:intergenic | 20.0% |
| !! | AAATTTCACAACATGTTATA+TGG | + | chr5.4:70088281-70088300 | MS.gene37572:CDS | 20.0% |
| !! | AACTGTTAATTATCAGAAAT+GGG | + | chr5.4:70086717-70086736 | MS.gene37572:CDS | 20.0% |
| !! | AATTAACAAACCAAACTTTA+GGG | - | chr5.4:70087640-70087659 | None:intergenic | 20.0% |
| !!! | ATTTTATGTTTAGACCTTTT+TGG | + | chr5.4:70086834-70086853 | MS.gene37572:intron | 20.0% |
| !!! | TTTGTCTCTTTTGTTAAAAA+AGG | + | chr5.4:70087499-70087518 | MS.gene37572:intron | 20.0% |
| !!! | TTTTTAACAAAAGAGACAAA+TGG | - | chr5.4:70087499-70087518 | None:intergenic | 20.0% |
| ! | ATAATCACATTGTTTGTATG+TGG | - | chr5.4:70087874-70087893 | None:intergenic | 25.0% |
| ! | CAACTGTTAATTATCAGAAA+TGG | + | chr5.4:70086716-70086735 | MS.gene37572:CDS | 25.0% |
| ! | CATTTCTGATAATTAACAGT+TGG | - | chr5.4:70086718-70086737 | None:intergenic | 25.0% |
| ! | CTAGTATTAACTTCAAAATG+TGG | + | chr5.4:70087721-70087740 | MS.gene37572:intron | 25.0% |
| ! | CTGATGATTATAATTGATTG+AGG | - | chr5.4:70087006-70087025 | None:intergenic | 25.0% |
| ! | GAATATTTGTACCAAAAGAA+AGG | - | chr5.4:70087594-70087613 | None:intergenic | 25.0% |
| ! | GTTTGTAATTGATTGTTTGA+TGG | + | chr5.4:70087083-70087102 | MS.gene37572:intron | 25.0% |
| ! | TAGAATAAAATGTCAACACT+GGG | + | chr5.4:70087146-70087165 | MS.gene37572:intron | 25.0% |
| ! | TCATGATATATGTTCCAAAA+AGG | - | chr5.4:70086851-70086870 | None:intergenic | 25.0% |
| ! | TGACAAAGATAAAGATAAAG+AGG | + | chr5.4:70088226-70088245 | MS.gene37572:CDS | 25.0% |
| ! | TTAGAATAAAATGTCAACAC+TGG | + | chr5.4:70087145-70087164 | MS.gene37572:intron | 25.0% |
| ! | TTCAAAATAAATGTGACTCA+TGG | + | chr5.4:70088053-70088072 | MS.gene37572:CDS | 25.0% |
| ! | TTGCATTGAATTACTTCTTA+TGG | + | chr5.4:70086916-70086935 | MS.gene37572:intron | 25.0% |
| !! | CTTTTTCTATCATGTTTCTT+AGG | + | chr5.4:70086625-70086644 | MS.gene37572:CDS | 25.0% |
| !! | TATATGTGTGACTTTTATGA+TGG | - | chr5.4:70087058-70087077 | None:intergenic | 25.0% |
| !! | TGTTAATCTGTTTTGCTAAA+TGG | - | chr5.4:70087460-70087479 | None:intergenic | 25.0% |
| !!! | ATGGATTCCTTGTTTTATTA+GGG | + | chr5.4:70088300-70088319 | MS.gene37572:CDS | 25.0% |
| !!! | TATGGATTCCTTGTTTTATT+AGG | + | chr5.4:70088299-70088318 | MS.gene37572:CDS | 25.0% |
| AAGATGTCCCTAATAAAACA+AGG | - | chr5.4:70088310-70088329 | None:intergenic | 30.0% | |
| ACACACAATTGAGTGTAAAT+TGG | - | chr5.4:70086978-70086997 | None:intergenic | 30.0% | |
| AGATAGAGATCATGTACTAT+AGG | - | chr5.4:70086885-70086904 | None:intergenic | 30.0% | |
| AGCTTACAAAAATTACACAG+TGG | + | chr5.4:70086657-70086676 | MS.gene37572:CDS | 30.0% | |
| GCTTACAAAAATTACACAGT+GGG | + | chr5.4:70086658-70086677 | MS.gene37572:CDS | 30.0% | |
| TACTCAAAATGCCAAAATGA+CGG | + | chr5.4:70087545-70087564 | MS.gene37572:intron | 30.0% | |
| TATATGTTTGAACAAGAGAG+TGG | - | chr5.4:70087845-70087864 | None:intergenic | 30.0% | |
| TCACAAGTTGTTTATGAAAG+TGG | + | chr5.4:70087190-70087209 | MS.gene37572:intron | 30.0% | |
| TGTATTCTTAGTGTGTCTTT+AGG | + | chr5.4:70087686-70087705 | MS.gene37572:intron | 30.0% | |
| TTCTTCTCTAGTGATTATGA+TGG | + | chr5.4:70088005-70088024 | MS.gene37572:CDS | 30.0% | |
| ! | TTCTCTTTTGATACTATGAG+TGG | + | chr5.4:70087611-70087630 | MS.gene37572:intron | 30.0% |
| !! | AAATTGGAAGCACTAAGAAA+AGG | + | chr5.4:70087356-70087375 | MS.gene37572:intron | 30.0% |
| !! | AATTGGAAGCACTAAGAAAA+GGG | + | chr5.4:70087357-70087376 | MS.gene37572:intron | 30.0% |
| !! | ACCTATTTTGGGAATAATGT+GGG | - | chr5.4:70087415-70087434 | None:intergenic | 30.0% |
| !! | ATGTTTTTATTTCCCCACTT+GGG | + | chr5.4:70087294-70087313 | MS.gene37572:intron | 30.0% |
| !! | TATGTTTTTATTTCCCCACT+TGG | + | chr5.4:70087293-70087312 | MS.gene37572:intron | 30.0% |
| ACCCACATTATTCCCAAAAT+AGG | + | chr5.4:70087411-70087430 | MS.gene37572:intron | 35.0% | |
| AGTTCCTTTACTGAAAGAAG+GGG | + | chr5.4:70087974-70087993 | MS.gene37572:CDS | 35.0% | |
| ATAAATGTGACTCATGGACA+AGG | + | chr5.4:70088059-70088078 | MS.gene37572:CDS | 35.0% | |
| CAGTTCCTTTACTGAAAGAA+GGG | + | chr5.4:70087973-70087992 | MS.gene37572:CDS | 35.0% | |
| CTTTAACATCGAAACAGAAC+TGG | - | chr5.4:70088255-70088274 | None:intergenic | 35.0% | |
| GAAGAGTGAATACTTGAGTT+GGG | - | chr5.4:70087438-70087457 | None:intergenic | 35.0% | |
| GATGGTGAACAATGCAAAAA+TGG | + | chr5.4:70088023-70088042 | MS.gene37572:CDS | 35.0% | |
| TAAATGTGACTCATGGACAA+GGG | + | chr5.4:70088060-70088079 | MS.gene37572:CDS | 35.0% | |
| TCATGTTTCTTAGGTTCTGT+TGG | + | chr5.4:70086634-70086653 | MS.gene37572:CDS | 35.0% | |
| TTAGGATGGTTGAAATTGGA+AGG | - | chr5.4:70088179-70088198 | None:intergenic | 35.0% | |
| TTCCAATTTCAACCATCCTA+AGG | + | chr5.4:70088178-70088197 | MS.gene37572:CDS | 35.0% | |
| TTCCTTAGGATGGTTGAAAT+TGG | - | chr5.4:70088183-70088202 | None:intergenic | 35.0% | |
| TTGGAAGGAATATTTGTGTC+TGG | - | chr5.4:70088164-70088183 | None:intergenic | 35.0% | |
| ! | TTATGATGATGCACAAGACA+AGG | + | chr5.4:70087890-70087909 | MS.gene37572:CDS | 35.0% |
| !! | GACCTATTTTGGGAATAATG+TGG | - | chr5.4:70087416-70087435 | None:intergenic | 35.0% |
| AAGACAAGGACACAATCCAA+TGG | + | chr5.4:70087904-70087923 | MS.gene37572:CDS | 40.0% | |
| AATGTCAACACTGGGAATAG+TGG | + | chr5.4:70087154-70087173 | MS.gene37572:intron | 40.0% | |
| ACTATAGTGTCACACCTAAG+TGG | + | chr5.4:70087269-70087288 | MS.gene37572:intron | 40.0% | |
| GAAGAGTCTTCAAAAACCGT+CGG | + | chr5.4:70088088-70088107 | MS.gene37572:CDS | 40.0% | |
| GACTGGATGTATATCAGTGT+TGG | - | chr5.4:70087946-70087965 | None:intergenic | 40.0% | |
| GCAGTTCCTTTACTGAAAGA+AGG | + | chr5.4:70087972-70087991 | MS.gene37572:CDS | 40.0% | |
| GCCAAGAAAGAATTCAGCTT+GGG | + | chr5.4:70086742-70086761 | MS.gene37572:CDS | 40.0% | |
| GGAAGAGTGAATACTTGAGT+TGG | - | chr5.4:70087439-70087458 | None:intergenic | 40.0% | |
| GGGATGTACTATTCTTGTTC+TGG | + | chr5.4:70087377-70087396 | MS.gene37572:intron | 40.0% | |
| TACACAGTGGGAGATTCTTT+AGG | + | chr5.4:70086670-70086689 | MS.gene37572:CDS | 40.0% | |
| TCCCAAGCTGAATTCTTTCT+TGG | - | chr5.4:70086746-70086765 | None:intergenic | 40.0% | |
| TGATATACATCCAGTCACAG+TGG | + | chr5.4:70087950-70087969 | MS.gene37572:CDS | 40.0% | |
| TGCCAAGAAAGAATTCAGCT+TGG | + | chr5.4:70086741-70086760 | MS.gene37572:CDS | 40.0% | |
| ! | GGATGTATATCAGTGTTGGA+CGG | - | chr5.4:70087942-70087961 | None:intergenic | 40.0% |
| !! | GACGGTTTTTGAAGACTCTT+CGG | - | chr5.4:70088089-70088108 | None:intergenic | 40.0% |
| !!! | ACTTGAGTTGGGACCTATTT+TGG | - | chr5.4:70087427-70087446 | None:intergenic | 40.0% |
| !!! | CTTGAGTTGGGACCTATTTT+GGG | - | chr5.4:70087426-70087445 | None:intergenic | 40.0% |
| AATGCCAAAATGACGGTTGC+TGG | + | chr5.4:70087552-70087571 | MS.gene37572:intron | 45.0% | |
| ACGGATCAACTGATGACCAT+TGG | - | chr5.4:70087923-70087942 | None:intergenic | 45.0% | |
| GACTCATCGTCACCAGAAAT+CGG | - | chr5.4:70088137-70088156 | None:intergenic | 45.0% | |
| GTTGCCCCTTCTTTCAGTAA+AGG | - | chr5.4:70087981-70088000 | None:intergenic | 45.0% | |
| TCATCGTCACTGTCTTCCTT+AGG | - | chr5.4:70088197-70088216 | None:intergenic | 45.0% | |
| TGAGTGGCTACCCTAAAGTT+TGG | + | chr5.4:70087627-70087646 | MS.gene37572:intron | 45.0% | |
| AAAGGAACTGCCACTGTGAC+TGG | - | chr5.4:70087963-70087982 | None:intergenic | 50.0% | |
| AATGCCACACATAGCCCAAG+TGG | - | chr5.4:70087311-70087330 | None:intergenic | 50.0% | |
| ATGCCACACATAGCCCAAGT+GGG | - | chr5.4:70087310-70087329 | None:intergenic | 50.0% | |
| CAGTGGGAGATTCTTTAGGC+TGG | + | chr5.4:70086674-70086693 | MS.gene37572:CDS | 50.0% | |
| CGTCACTGTCTTCCTTAGGA+TGG | - | chr5.4:70088193-70088212 | None:intergenic | 50.0% | |
| GGAGATGGAGAATCTTCCGA+CGG | - | chr5.4:70088107-70088126 | None:intergenic | 50.0% | |
| GTCGGAAGATTCTCCATCTC+CGG | + | chr5.4:70088106-70088125 | MS.gene37572:CDS | 50.0% | |
| ! | AGGTCCAGCAACCGTCATTT+TGG | - | chr5.4:70087559-70087578 | None:intergenic | 50.0% |
| !! | TCACCAGAAATCGGACTAGC+CGG | - | chr5.4:70088128-70088147 | None:intergenic | 50.0% |
| TGCCACACATAGCCCAAGTG+GGG | - | chr5.4:70087309-70087328 | None:intergenic | 55.0% | |
| TTCCCCACTTGGGCTATGTG+TGG | + | chr5.4:70087304-70087323 | MS.gene37572:intron | 55.0% | |
| ! | TCTCCGGCTAGTCCGATTTC+TGG | + | chr5.4:70088122-70088141 | MS.gene37572:CDS | 55.0% |
| !! | GAAATCGGACTAGCCGGAGA+TGG | - | chr5.4:70088122-70088141 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 70086574 | 70088340 | 70086574 | ID=MS.gene37572 |
| chr5.4 | mRNA | 70086574 | 70088340 | 70086574 | ID=MS.gene37572.t1;Parent=MS.gene37572 |
| chr5.4 | exon | 70086574 | 70086775 | 70086574 | ID=MS.gene37572.t1.exon1;Parent=MS.gene37572.t1 |
| chr5.4 | CDS | 70086574 | 70086775 | 70086574 | ID=cds.MS.gene37572.t1;Parent=MS.gene37572.t1 |
| chr5.4 | exon | 70087823 | 70088340 | 70087823 | ID=MS.gene37572.t1.exon2;Parent=MS.gene37572.t1 |
| chr5.4 | CDS | 70087823 | 70088340 | 70087823 | ID=cds.MS.gene37572.t1;Parent=MS.gene37572.t1 |
| Gene Sequence |
| Protein sequence |
