AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene056092

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056092.t1 MTR_1g083750 97.400 423 11 0 1 423 1 423 0.0 846
MS.gene056092.t1 MTR_7g112430 85.071 422 61 2 1 422 1 420 0.0 717
MS.gene056092.t1 MTR_1g083750 96.979 331 10 0 93 423 1 331 0.0 658
MS.gene056092.t1 MTR_1g110210 73.065 323 85 1 1 323 1 321 5.17e-173 487
MS.gene056092.t1 MTR_7g029240 62.673 434 137 13 1 422 1 421 1.34e-167 477
MS.gene056092.t1 MTR_1g019760 56.098 410 178 2 14 423 11 418 4.63e-167 476
MS.gene056092.t1 MTR_3g101540 55.556 414 175 3 14 422 10 419 4.08e-163 466
MS.gene056092.t1 MTR_3g101540 55.556 414 175 3 14 422 10 419 5.13e-163 466
MS.gene056092.t1 MTR_1g019760 58.671 346 141 2 14 359 11 354 5.08e-148 427
MS.gene056092.t1 MTR_1g014640 31.449 283 155 12 25 298 72 324 2.14e-28 114
MS.gene056092.t1 MTR_1g013400 31.449 283 155 12 25 298 72 324 2.14e-28 114
MS.gene056092.t1 MTR_3g105730 31.452 248 144 9 57 298 32 259 3.09e-27 110
MS.gene056092.t1 MTR_3g105730 30.924 249 144 9 57 298 54 281 3.87e-27 110
MS.gene056092.t1 MTR_1g115570 31.673 281 155 14 25 299 90 339 1.72e-25 106
MS.gene056092.t1 MTR_3g464650 29.779 272 163 11 33 297 64 314 5.14e-25 104
MS.gene056092.t1 MTR_3g464700 31.579 228 136 8 33 260 88 295 2.20e-24 103
MS.gene056092.t1 MTR_5g071550 28.625 269 164 11 36 297 127 374 2.44e-22 98.2
MS.gene056092.t1 MTR_8g463130 30.043 233 139 8 68 295 66 279 3.58e-21 93.2
MS.gene056092.t1 MTR_8g463130 30.043 233 139 8 68 295 38 251 4.45e-21 92.4
MS.gene056092.t1 MTR_2g078760 29.572 257 152 11 46 297 21 253 3.86e-20 89.7
MS.gene056092.t1 MTR_2g078760 29.572 257 152 11 46 297 100 332 4.14e-20 91.3
MS.gene056092.t1 MTR_4g125810 27.365 296 159 10 46 297 220 503 7.72e-20 92.0
MS.gene056092.t1 MTR_3g091060 29.020 255 152 9 46 295 108 338 2.88e-19 89.0
MS.gene056092.t1 MTR_4g094208 27.698 278 168 10 27 295 21 274 5.66e-19 87.0
MS.gene056092.t1 MTR_1g050520 31.301 246 136 7 69 299 419 646 6.49e-19 89.7
MS.gene056092.t1 MTR_5g071550 28.636 220 137 8 36 255 127 326 9.51e-19 87.4
MS.gene056092.t1 MTR_4g123080 29.699 266 151 10 67 313 94 342 1.09e-18 87.4
MS.gene056092.t1 MTR_4g123080 30.556 252 139 10 67 299 112 346 1.47e-18 87.0
MS.gene056092.t1 MTR_5g024340 26.894 264 153 9 67 314 123 362 2.04e-18 86.7
MS.gene056092.t1 MTR_3g074610 29.032 279 163 11 27 295 26 279 3.77e-18 84.7
MS.gene056092.t1 MTR_2g008850 25.818 275 177 9 27 295 25 278 4.81e-18 84.3
MS.gene056092.t1 MTR_2g008850 27.778 234 143 9 68 295 19 232 7.02e-18 82.8
MS.gene056092.t1 MTR_5g063940 29.137 278 164 12 27 295 26 279 1.23e-17 83.2
MS.gene056092.t1 MTR_5g063940 30.472 233 138 9 68 295 19 232 1.94e-17 81.6
MS.gene056092.t1 MTR_5g009370 29.524 210 123 7 66 265 85 279 2.20e-17 83.2
MS.gene056092.t1 MTR_1g015110 30.712 267 137 10 69 299 291 545 1.32e-16 82.4
MS.gene056092.t1 MTR_4g094542 29.921 254 150 10 46 295 133 362 2.15e-16 80.5
MS.gene056092.t1 MTR_3g068200 29.032 217 122 6 62 261 117 318 2.23e-16 80.9
MS.gene056092.t1 MTR_2g078760 30.332 211 120 9 46 252 100 287 2.65e-16 79.3
MS.gene056092.t1 MTR_2g040500 25.857 321 162 10 40 297 228 535 2.83e-16 81.3
MS.gene056092.t1 MTR_4g116420 29.762 252 132 10 65 295 121 348 5.44e-16 79.7
MS.gene056092.t1 MTR_1g086350 28.358 268 156 11 66 314 14 264 6.75e-16 78.2
MS.gene056092.t1 MTR_8g102550 29.442 197 118 6 69 258 153 335 7.03e-16 79.3
MS.gene056092.t1 MTR_2g033000 31.373 255 131 11 64 295 96 329 7.24e-16 79.0
MS.gene056092.t1 MTR_6g081850 28.053 303 165 13 26 295 34 316 8.47e-16 78.2
MS.gene056092.t1 MTR_3g491830 24.633 341 185 14 29 322 54 369 1.11e-15 78.6
MS.gene056092.t1 MTR_3g104710 27.574 272 149 8 62 297 280 539 1.35e-15 79.3
MS.gene056092.t1 MTR_1g086350 28.464 267 155 11 67 314 101 350 1.79e-15 77.8
MS.gene056092.t1 MTR_1g086350 28.464 267 155 11 67 314 117 366 2.34e-15 77.8
MS.gene056092.t1 MTR_5g042243 85.366 41 6 0 1 41 1 41 3.19e-15 72.0
MS.gene056092.t1 MTR_7g112490 33.898 177 95 9 116 276 183 353 9.80e-15 75.9
MS.gene056092.t1 MTR_7g112490 33.898 177 95 9 116 276 165 335 1.38e-14 75.1
MS.gene056092.t1 MTR_1g028300 31.048 248 138 9 69 297 146 379 1.45e-14 75.1
MS.gene056092.t1 MTR_7g021530 30.403 273 142 14 36 283 48 297 2.98e-14 73.9
MS.gene056092.t1 MTR_5g080680 28.019 207 123 7 69 262 133 326 3.43e-14 74.3
MS.gene056092.t1 MTR_7g021530 30.403 273 142 14 36 283 98 347 7.11e-14 73.2
MS.gene056092.t1 MTR_7g021530 30.403 273 142 14 36 283 139 388 1.29e-13 72.8
MS.gene056092.t1 MTR_6g087000 27.517 298 167 14 36 297 97 381 2.28e-13 72.8
MS.gene056092.t1 MTR_6g087000 27.517 298 167 14 36 297 97 381 2.30e-13 72.8
MS.gene056092.t1 MTR_3g107880 27.969 261 145 9 67 308 120 356 2.65e-13 71.2
MS.gene056092.t1 MTR_3g107880 27.969 261 145 9 67 308 119 355 2.76e-13 71.2
MS.gene056092.t1 MTR_3g107880 27.969 261 145 9 67 308 62 298 3.25e-13 70.5
MS.gene056092.t1 MTR_4g118340 31.126 302 148 19 36 297 59 340 6.45e-13 70.5
MS.gene056092.t1 MTR_8g017240 29.949 197 113 9 100 283 136 320 6.65e-13 70.1
MS.gene056092.t1 MTR_2g020970 26.829 287 166 11 43 297 67 341 2.33e-12 68.2
MS.gene056092.t1 MTR_4g007440 34.043 141 66 5 128 257 156 280 4.83e-12 67.4
MS.gene056092.t1 MTR_2g020970 26.829 287 166 11 43 297 67 341 6.28e-12 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056092.t1 AT1G09160 75.355 422 102 1 1 422 1 420 0.0 647
MS.gene056092.t1 AT1G09160 75.355 422 102 1 1 422 1 420 0.0 647
MS.gene056092.t1 AT1G68410 68.434 415 127 2 11 422 16 429 0.0 577
MS.gene056092.t1 AT1G68410 68.434 415 127 2 11 422 16 429 0.0 577
MS.gene056092.t1 AT1G68410 68.434 415 127 2 11 422 16 429 0.0 577
MS.gene056092.t1 AT1G68410 68.434 415 127 2 11 422 16 429 0.0 577
MS.gene056092.t1 AT1G47380 56.341 410 178 1 14 423 10 418 4.95e-170 483
MS.gene056092.t1 AT5G10740 30.921 304 168 13 6 298 8 280 4.25e-27 111
MS.gene056092.t1 AT5G10740 30.921 304 168 13 6 298 8 280 4.25e-27 111
MS.gene056092.t1 AT2G34740 33.054 239 136 8 65 298 116 335 7.91e-27 110
MS.gene056092.t1 AT1G07160 30.686 277 157 11 33 297 123 376 1.27e-25 107
MS.gene056092.t1 AT1G67820 32.787 244 139 10 34 270 78 303 1.44e-25 108
MS.gene056092.t1 AT5G24940 30.877 285 154 13 25 298 28 280 1.76e-25 108
MS.gene056092.t1 AT1G67820 32.787 244 139 10 34 270 114 339 1.89e-25 108
MS.gene056092.t1 AT4G31750 30.108 279 158 11 25 295 28 277 3.23e-25 105
MS.gene056092.t1 AT4G31750 30.357 280 156 12 25 295 28 277 3.88e-25 105
MS.gene056092.t1 AT4G31750 30.357 280 156 12 25 295 28 277 3.88e-25 105
MS.gene056092.t1 AT1G43900 31.970 269 148 14 36 295 124 366 3.61e-24 103
MS.gene056092.t1 AT1G22280 30.901 233 143 7 68 295 65 284 4.74e-24 101
MS.gene056092.t1 AT2G40180 28.269 283 183 8 21 297 117 385 9.74e-24 102
MS.gene056092.t1 AT1G34750 29.749 279 161 11 27 295 26 279 1.58e-23 100
MS.gene056092.t1 AT1G34750 29.749 279 161 11 27 295 26 279 1.58e-23 100
MS.gene056092.t1 AT1G34750 29.749 279 161 11 27 295 26 279 1.58e-23 100
MS.gene056092.t1 AT1G78200 30.357 280 161 11 27 295 24 280 1.97e-23 99.8
MS.gene056092.t1 AT1G78200 30.357 280 161 11 27 295 24 280 1.97e-23 99.8
MS.gene056092.t1 AT1G78200 30.357 280 161 11 27 295 24 280 1.97e-23 99.8
MS.gene056092.t1 AT1G78200 31.128 257 147 10 46 295 2 235 2.20e-23 98.6
MS.gene056092.t1 AT1G78200 31.128 257 147 10 46 295 2 235 2.20e-23 98.6
MS.gene056092.t1 AT4G28400 29.258 229 146 6 68 295 45 258 8.37e-23 97.4
MS.gene056092.t1 AT2G30020 28.782 271 163 11 36 297 143 392 1.07e-22 99.8
MS.gene056092.t1 AT5G53140 29.412 255 151 10 46 295 115 345 1.14e-22 99.8
MS.gene056092.t1 AT5G53140 29.412 255 151 10 46 295 115 345 1.14e-22 99.8
MS.gene056092.t1 AT4G28400 29.258 229 146 6 68 295 67 280 1.44e-22 97.4
MS.gene056092.t1 AT1G22280 30.901 233 137 8 68 295 65 278 3.40e-22 96.3
MS.gene056092.t1 AT2G20630 29.104 268 158 12 33 295 36 276 5.52e-22 95.5
MS.gene056092.t1 AT4G26080 27.737 274 149 7 61 299 165 424 1.33e-21 96.7
MS.gene056092.t1 AT3G51470 28.475 295 169 11 36 314 32 300 2.00e-21 94.7
MS.gene056092.t1 AT2G20630 28.839 267 158 12 33 294 36 275 2.17e-21 94.0
MS.gene056092.t1 AT3G51470 28.475 295 169 11 36 314 76 344 2.33e-21 95.1
MS.gene056092.t1 AT5G57050 28.464 267 145 9 65 297 117 371 3.87e-20 92.0
MS.gene056092.t1 AT5G57050 28.464 267 145 9 65 297 157 411 4.97e-20 92.0
MS.gene056092.t1 AT2G40860 29.286 280 157 9 36 299 396 650 1.47e-19 91.7
MS.gene056092.t1 AT2G25620 30.451 266 136 10 67 308 127 367 1.48e-19 90.5
MS.gene056092.t1 AT2G25620 30.451 266 136 10 67 308 127 367 1.48e-19 90.5
MS.gene056092.t1 AT2G40860 29.286 280 157 9 36 299 298 552 2.46e-19 90.9
MS.gene056092.t1 AT2G34740 29.665 209 127 5 65 270 112 303 2.68e-19 88.6
MS.gene056092.t1 AT2G34740 29.665 209 127 5 65 270 130 321 3.17e-19 88.6
MS.gene056092.t1 AT3G62260 29.151 271 157 12 67 319 26 279 1.14e-17 83.6
MS.gene056092.t1 AT2G40860 28.409 264 148 8 36 282 396 635 1.20e-17 85.9
MS.gene056092.t1 AT1G48040 32.692 260 129 12 63 295 107 347 2.08e-17 84.0
MS.gene056092.t1 AT3G62260 29.151 271 157 12 67 319 116 369 4.77e-17 82.8
MS.gene056092.t1 AT3G62260 29.151 271 157 12 67 319 115 368 5.06e-17 82.8
MS.gene056092.t1 AT3G15260 29.185 233 141 8 68 295 73 286 5.35e-17 81.3
MS.gene056092.t1 AT3G15260 29.185 233 141 8 68 295 73 286 5.35e-17 81.3
MS.gene056092.t1 AT1G34750 28.085 235 136 9 27 252 26 236 1.26e-16 79.3
MS.gene056092.t1 AT5G59220 28.287 251 150 10 31 265 107 343 2.92e-15 77.8
MS.gene056092.t1 AT4G28400 27.568 185 120 4 68 252 67 237 4.41e-15 75.1
MS.gene056092.t1 AT1G18030 29.020 255 135 10 69 295 110 346 5.41e-15 76.3
MS.gene056092.t1 AT1G07430 28.054 221 126 7 62 265 151 355 4.92e-14 73.9
MS.gene056092.t1 AT4G27800 24.848 330 173 12 40 322 67 368 1.37e-13 72.4
MS.gene056092.t1 AT3G11410 25.446 224 126 7 62 260 131 338 2.28e-12 68.6
MS.gene056092.t1 AT2G40860 29.114 237 127 8 36 255 396 608 3.20e-12 68.9
MS.gene056092.t1 AT1G16220 23.864 352 195 13 5 301 45 378 3.92e-12 68.2
MS.gene056092.t1 AT3G17250 28.063 253 140 13 67 295 157 391 4.18e-12 68.2
MS.gene056092.t1 AT4G27800 26.119 268 134 9 40 265 67 312 4.30e-12 67.4
MS.gene056092.t1 AT4G27800 26.119 268 134 9 40 265 67 312 4.34e-12 67.4
MS.gene056092.t1 AT1G79630 29.798 198 121 6 131 314 151 344 7.89e-12 67.0
MS.gene056092.t1 AT1G79630 29.798 198 121 6 131 314 151 344 7.89e-12 67.0
MS.gene056092.t1 AT5G27930 33.544 158 88 7 105 251 150 301 9.00e-12 66.2
MS.gene056092.t1 AT1G79630 29.500 200 123 6 131 316 111 306 1.01e-11 66.6
MS.gene056092.t1 AT1G79630 29.500 200 123 6 131 316 111 306 1.01e-11 66.6
MS.gene056092.t1 AT1G79630 29.500 200 123 6 131 316 111 306 1.01e-11 66.6
MS.gene056092.t1 AT1G79630 29.500 200 123 6 131 316 111 306 1.01e-11 66.6
MS.gene056092.t1 AT1G79630 29.500 200 123 6 131 316 217 412 1.05e-11 67.0
MS.gene056092.t1 AT1G17550 27.434 226 120 8 69 265 238 448 1.13e-11 67.0
MS.gene056092.t1 AT1G17550 27.434 226 120 8 69 265 238 448 1.13e-11 67.0
MS.gene056092.t1 AT1G79630 29.500 200 123 6 131 316 221 416 1.38e-11 66.6
MS.gene056092.t1 AT4G32950 30.114 176 110 5 131 297 138 309 4.10e-11 64.3
MS.gene056092.t1 AT5G51760 25.301 249 152 6 40 265 86 323 4.21e-11 64.7
MS.gene056092.t1 AT2G29380 27.928 222 132 8 59 265 103 311 4.50e-11 64.3
MS.gene056092.t1 AT1G18030 30.288 208 104 9 69 253 110 299 4.81e-11 63.9
MS.gene056092.t1 AT5G51760 25.301 249 152 6 40 265 116 353 5.20e-11 64.7
MS.gene056092.t1 AT5G36250 26.222 225 136 9 100 300 171 389 5.33e-11 64.7
MS.gene056092.t1 AT1G72770 25.090 279 143 11 69 297 239 501 5.48e-11 64.7
MS.gene056092.t1 AT1G72770 25.090 279 143 11 69 297 239 501 5.48e-11 64.7
MS.gene056092.t1 AT1G72770 25.090 279 143 11 69 297 239 501 5.48e-11 64.7
MS.gene056092.t1 AT2G25070 31.206 141 70 5 128 257 156 280 8.87e-11 63.5
MS.gene056092.t1 AT2G25070 31.206 141 70 5 128 257 156 280 8.87e-11 63.5

Find 119 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATAGCTTCTGATGGAATT+TGG 0.123861 1.4:-67724286 MS.gene056092:CDS
AGCACATTATGTTTCTTTCT+TGG 0.197447 1.4:+67723992 None:intergenic
GTTCCTTCCAATACAAATTC+TGG 0.255485 1.4:-67723357 MS.gene056092:CDS
TGTTTCTTTCTTGGAGTTGC+TGG 0.268275 1.4:+67724001 None:intergenic
GTGCTTAGTTCACTTCTCTT+TGG 0.288508 1.4:-67723974 MS.gene056092:CDS
TTACCTGCAGGGGAAACTTC+TGG 0.307065 1.4:-67724829 MS.gene056092:intron
TATAGCTTCTGATGGAATTT+GGG 0.324404 1.4:-67724285 MS.gene056092:CDS
GCTACGTTTGTGATTATTGA+TGG 0.331297 1.4:-67724799 MS.gene056092:CDS
CCGTTACAGTTGCATCTGTT+GGG 0.351128 1.4:-67724771 MS.gene056092:CDS
ACAGATTGCCATAGAGTTCC+TGG 0.368715 1.4:-67725449 MS.gene056092:CDS
ACTTGCTTAACATGTGGTAT+TGG 0.372029 1.4:+67724442 None:intergenic
TGCTGCCAAGCTGGTGGTTA+AGG 0.391611 1.4:-67724207 MS.gene056092:intron
AAGACGCCATGGAAGGAAAA+AGG 0.416517 1.4:-67723200 MS.gene056092:CDS
CAGATCCTTGACGGGCATAA+TGG 0.417208 1.4:-67725077 MS.gene056092:CDS
AACTTGAACAGGAAGCTCTA+AGG 0.419981 1.4:-67724099 MS.gene056092:intron
GGGCCTCTTCGCTGTTGGCC+TGG 0.420476 1.4:-67724520 MS.gene056092:CDS
GTAGGAAGACTCAACGTATT+CGG 0.426631 1.4:-67724634 MS.gene056092:CDS
CTTGATTGGCAGATCCTTGA+CGG 0.435403 1.4:-67725086 MS.gene056092:intron
TGGAAGAAAATGTAGAAGAG+AGG 0.437691 1.4:-67724684 MS.gene056092:CDS
GTGAATACCAGAATTTGTAT+TGG 0.439513 1.4:+67723350 None:intergenic
CTTAAAATGCAGGCTAGGTA+AGG 0.443855 1.4:-67723382 MS.gene056092:intron
ATGGCTTCAAGCACTGCCTC+GGG 0.448779 1.4:-67724973 MS.gene056092:CDS
ACTAATAAGCTTTCTGCAGT+TGG 0.455037 1.4:-67723920 MS.gene056092:CDS
TCGTAGCTCACGGCCGATCA+AGG 0.456909 1.4:+67725553 None:intergenic
GTTGCTGGCATCTGCAACAC+AGG 0.461352 1.4:+67724016 None:intergenic
GGATACTTTGTCTTCTGATA+CGG 0.461894 1.4:-67724264 MS.gene056092:CDS
AATGGCTTCAAGCACTGCCT+CGG 0.462446 1.4:-67724974 MS.gene056092:CDS
ATCGGCCGTGAGCTACGAAA+TGG 0.463670 1.4:-67725548 MS.gene056092:CDS
GTTCCGTTGGCAACCTTGAT+CGG 0.465033 1.4:-67725566 MS.gene056092:CDS
CCATACCGCAAGGTGTAAGC+AGG 0.465666 1.4:-67725001 MS.gene056092:CDS
AAGAATCGCCAGGAACTCTA+TGG 0.471757 1.4:+67725441 None:intergenic
GCCTGTCCATACTTCACAAA+AGG 0.476504 1.4:+67725512 None:intergenic
GAAGTATGGACAGGCTGGTT+TGG 0.479502 1.4:-67725504 MS.gene056092:CDS
GTGGAGGAGTTATTTGAAGA+GGG 0.481037 1.4:-67723893 MS.gene056092:CDS
CTGGGTATCTAATATGCATC+GGG 0.481436 1.4:+67724744 None:intergenic
ACCGTTACAGTTGCATCTGT+TGG 0.483048 1.4:-67724772 MS.gene056092:CDS
TGCAGAACTTGCTGCCAAGC+TGG 0.486136 1.4:-67724216 MS.gene056092:CDS
CCTGCTTACACCTTGCGGTA+TGG 0.486759 1.4:+67725001 None:intergenic
TCTTCCTACTTCACCACCAC+TGG 0.489968 1.4:+67724648 None:intergenic
AATAATATCTACAACAAGGC+AGG 0.494608 1.4:+67724051 None:intergenic
GCTTTCTGCAGTTGGTATTG+TGG 0.496447 1.4:-67723912 MS.gene056092:CDS
TGACAGTTTGTGCAGAGGAA+TGG 0.499633 1.4:+67723231 None:intergenic
CCTTCCCATGGCTTAGATAC+CGG 0.499863 1.4:+67723255 None:intergenic
TGCCTAGCAGATCAACCATC+TGG 0.501426 1.4:-67723315 MS.gene056092:CDS
TTTGTGAAGTATGGACAGGC+TGG 0.503204 1.4:-67725509 MS.gene056092:CDS
GTTTCCTAGCTTTCAAATGC+GGG 0.505250 1.4:-67724320 MS.gene056092:intron
AAAATGTAGAAGAGAGGGAG+CGG 0.505425 1.4:-67724678 MS.gene056092:CDS
CGTTTGTGATTATTGATGGA+TGG 0.510062 1.4:-67724795 MS.gene056092:CDS
AGAGAGAAACAACACCTCCC+TGG 0.510635 1.4:+67724725 None:intergenic
TTCTGCAGTTGGTATTGTGG+AGG 0.515246 1.4:-67723909 MS.gene056092:CDS
AGCTTGGCAGCAAGTTCTGC+AGG 0.516289 1.4:+67724218 None:intergenic
AGGTAGGGCCTCTTCGCTGT+TGG 0.519693 1.4:-67724525 MS.gene056092:CDS
ACAGCCAGTGGTGGTGAAGT+AGG 0.520024 1.4:-67724652 MS.gene056092:CDS
CGTTACAGTTGCATCTGTTG+GGG 0.531574 1.4:-67724770 MS.gene056092:CDS
TTGATTGGCAGATCCTTGAC+GGG 0.533037 1.4:-67725085 MS.gene056092:intron
GAAGCAACCACTTGTTCCGT+TGG 0.533409 1.4:-67725579 MS.gene056092:CDS
TGAGTAAGACTGAATTATCA+AGG 0.534598 1.4:-67725604 MS.gene056092:CDS
ATAAAATGATCGGTGTTCAC+TGG 0.535304 1.4:+67723282 None:intergenic
CCTCTTCGCTGTTGGCCTGG+TGG 0.536837 1.4:-67724517 MS.gene056092:CDS
AAATGTAGAAGAGAGGGAGC+GGG 0.539423 1.4:-67724677 MS.gene056092:CDS
TGTGGAGGAGTTATTTGAAG+AGG 0.540145 1.4:-67723894 MS.gene056092:CDS
AATGACGTTGAAGAATCGCC+AGG 0.544088 1.4:+67725431 None:intergenic
CCTGGGTATCTAATATGCAT+CGG 0.544206 1.4:+67724743 None:intergenic
ATACCAGAATTTGTATTGGA+AGG 0.544653 1.4:+67723354 None:intergenic
TCCTCCCTGCTTACACCTTG+CGG 0.545928 1.4:+67724996 None:intergenic
TCACTCCGGTATCTAAGCCA+TGG 0.550001 1.4:-67723260 MS.gene056092:CDS
AGACTAATTATAGCTTCTGA+TGG 0.551958 1.4:-67724293 MS.gene056092:CDS
TCTAAGGTCAAGAGGGCTGA+AGG 0.552829 1.4:-67724083 MS.gene056092:CDS
TGTCACCAATCGATCTAGAA+AGG 0.554127 1.4:+67724488 None:intergenic
GAGAGAAACAACACCTCCCT+GGG 0.554466 1.4:+67724726 None:intergenic
AGGGAGCGGGTTACAGCCAG+TGG 0.559334 1.4:-67724664 MS.gene056092:CDS
GGAAGAAAATGTAGAAGAGA+GGG 0.560448 1.4:-67724683 MS.gene056092:CDS
CACTCCGGTATCTAAGCCAT+GGG 0.564768 1.4:-67723259 MS.gene056092:CDS
TGTTTCCTAGCTTTCAAATG+CGG 0.566362 1.4:-67724321 MS.gene056092:intron
AAGGTGTAAGCAGGGAGGAA+TGG 0.566433 1.4:-67724992 MS.gene056092:CDS
CTAGAAAGGCACAATCCACC+AGG 0.566834 1.4:+67724502 None:intergenic
CCGATGCATATTAGATACCC+AGG 0.567386 1.4:-67724743 MS.gene056092:CDS
TCTTCTGACAGTTTGTGCAG+AGG 0.570739 1.4:+67723226 None:intergenic
TTGTGCCTTTCTAGATCGAT+TGG 0.574242 1.4:-67724493 MS.gene056092:CDS
CACCAGATGGTTGATCTGCT+AGG 0.577655 1.4:+67723313 None:intergenic
AGGTACACCACGACATGACT+TGG 0.578313 1.4:+67724238 None:intergenic
CAGGAAGCTCTAAGGTCAAG+AGG 0.580501 1.4:-67724091 MS.gene056092:CDS
ACCTTCACTTGCTTAACATG+TGG 0.582513 1.4:+67724436 None:intergenic
CAGAGGAATGGACCTTCCCA+TGG 0.585428 1.4:+67723243 None:intergenic
TCTCAAAACTCGACAAACAA+AGG 0.587947 1.4:-67723944 MS.gene056092:CDS
CTTGACAGTTGATCACAGAC+TGG 0.593422 1.4:-67724704 MS.gene056092:CDS
AAGAAGAAAGACGCCATGGA+AGG 0.594109 1.4:-67723207 MS.gene056092:CDS
ATCGATTGGTGACACCGATG+TGG 0.601496 1.4:-67724479 MS.gene056092:CDS
GCAGATCAACCATCTGGTGA+TGG 0.602774 1.4:-67723309 MS.gene056092:CDS
TACCGGAGTGATAAAATGAT+CGG 0.613081 1.4:+67723272 None:intergenic
CATCACCTTAACCACCAGCT+TGG 0.613564 1.4:+67724202 None:intergenic
CCACCAGGCCAACAGCGAAG+AGG 0.616089 1.4:+67724517 None:intergenic
TGCAACACAGGATAGTCTGA+AGG 0.617016 1.4:+67724028 None:intergenic
TCGATTGGTGACACCGATGT+GGG 0.617709 1.4:-67724478 MS.gene056092:CDS
AAGGTTGCCAACGGAACAAG+TGG 0.618923 1.4:+67725572 None:intergenic
ATATACCATTATGCCCGTCA+AGG 0.625718 1.4:+67725072 None:intergenic
GAGCGGGTTACAGCCAGTGG+TGG 0.626341 1.4:-67724661 MS.gene056092:CDS
AGGAAGCTCTAAGGTCAAGA+GGG 0.627250 1.4:-67724090 MS.gene056092:CDS
ACCACATGTTAAGCAAGTGA+AGG 0.627901 1.4:-67724437 MS.gene056092:intron
GTCATGAGTGCCATACCGCA+AGG 0.630194 1.4:-67725011 MS.gene056092:CDS
TCAGAAGAAGAAAGACGCCA+TGG 0.630309 1.4:-67723211 MS.gene056092:CDS
CCCAACAGATGCAACTGTAA+CGG 0.630410 1.4:+67724771 None:intergenic
CGGCCGATCAAGGTTGCCAA+CGG 0.631386 1.4:+67725563 None:intergenic
CCGGTATCTAAGCCATGGGA+AGG 0.632107 1.4:-67723255 MS.gene056092:CDS
AAAGCGACAACTAGAGCCCG+AGG 0.640273 1.4:+67724957 None:intergenic
CAACGTATTCGGAGGCAATG+AGG 0.642352 1.4:-67724623 MS.gene056092:intron
CGATGCATATTAGATACCCA+GGG 0.643134 1.4:-67724742 MS.gene056092:CDS
TCCTAGCTTTCAAATGCGGG+CGG 0.652825 1.4:-67724317 MS.gene056092:intron
ACTGGTAAACCATCACCAGA+TGG 0.656343 1.4:+67723300 None:intergenic
CATACCGCAAGGTGTAAGCA+GGG 0.674683 1.4:-67725000 MS.gene056092:CDS
GGAAGACTCAACGTATTCGG+AGG 0.678380 1.4:-67724631 MS.gene056092:CDS
AACGATATACTCTCCCACAT+CGG 0.681964 1.4:+67724465 None:intergenic
AGAACTTGCTGCCAAGCTGG+TGG 0.683075 1.4:-67724213 MS.gene056092:CDS
ACGGCTGCCAAGTCATGTCG+TGG 0.691102 1.4:-67724245 MS.gene056092:CDS
AAGGAATAATATCTACAACA+AGG 0.708500 1.4:+67724047 None:intergenic
ACCGCAAGGTGTAAGCAGGG+AGG 0.718559 1.4:-67724997 MS.gene056092:CDS
GTTCTGCAATGCTTACAGAG+AGG 0.751165 1.4:-67723871 MS.gene056092:intron
TGCATATTAGATACCCAGGG+AGG 0.753250 1.4:-67724739 MS.gene056092:CDS
CTGCTAGGCAAACTGCACAG+CGG 0.759343 1.4:+67723328 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCAATTTTTACTTTTTACAT+CGG + chr1.4:67725319-67725338 None:intergenic 15.0%
!!! TTGAAAATGCATTTTATAAA+TGG - chr1.4:67725134-67725153 MS.gene056092:intron 15.0%
!! AAGAACTACTAAACTTTAAA+AGG + chr1.4:67724996-67725015 None:intergenic 20.0%
!! ATAAGATCTTATGAATGAAT+TGG - chr1.4:67723528-67723547 MS.gene056092:intron 20.0%
!! TAAGGAAGAAATTATATGAA+AGG + chr1.4:67724224-67724243 None:intergenic 20.0%
!!! AAAATTTAATTTTACCTGCA+GGG - chr1.4:67723959-67723978 MS.gene056092:CDS 20.0%
!!! CATTTGATTATGAATCTTTT+TGG + chr1.4:67725261-67725280 None:intergenic 20.0%
!!! TAAAATTTAATTTTACCTGC+AGG - chr1.4:67723958-67723977 MS.gene056092:CDS 20.0%
!!! TTTTTGTTTTCTTAAAATGC+AGG - chr1.4:67725407-67725426 MS.gene056092:intron 20.0%
! AAGGAATAATATCTACAACA+AGG + chr1.4:67724755-67724774 None:intergenic 25.0%
! ATCAATAAGGAATTCTTGAT+TGG - chr1.4:67723699-67723718 MS.gene056092:intron 25.0%
! ATTGATATCATAACCATTGT+GGG + chr1.4:67723685-67723704 None:intergenic 25.0%
! CAATGGTTATGATATCAATA+AGG - chr1.4:67723686-67723705 MS.gene056092:intron 25.0%
! GATTGGAACAAAAATGTTTA+GGG - chr1.4:67725036-67725055 MS.gene056092:CDS 25.0%
! TATTGATATCATAACCATTG+TGG + chr1.4:67723686-67723705 None:intergenic 25.0%
! TGATTGGAACAAAAATGTTT+AGG - chr1.4:67725035-67725054 MS.gene056092:CDS 25.0%
! TTCCTTAATATGTTTAGGTA+GGG - chr1.4:67724259-67724278 MS.gene056092:CDS 25.0%
!! AGTTTTCTTTGTATCCTTAT+TGG - chr1.4:67723907-67723926 MS.gene056092:CDS 25.0%
!! TTTGGTTCCTTAATATGTTT+AGG - chr1.4:67724254-67724273 MS.gene056092:CDS 25.0%
!!! AAATTTAATTTTACCTGCAG+GGG - chr1.4:67723960-67723979 MS.gene056092:CDS 25.0%
!!! TCTATTGTTTTAACTTGAAC+AGG - chr1.4:67724689-67724708 MS.gene056092:CDS 25.0%
AAAGGGAATGTATCTTTCTT+AGG - chr1.4:67723575-67723594 MS.gene056092:intron 30.0%
AATAATATCTACAACAAGGC+AGG + chr1.4:67724751-67724770 None:intergenic 30.0%
AGACTAATTATAGCTTCTGA+TGG - chr1.4:67724506-67724525 MS.gene056092:CDS 30.0%
AGCACATTATGTTTCTTTCT+TGG + chr1.4:67724810-67724829 None:intergenic 30.0%
AGTAAACACCTTCACATAAA+AGG + chr1.4:67724425-67724444 None:intergenic 30.0%
ATACCAGAATTTGTATTGGA+AGG + chr1.4:67725448-67725467 None:intergenic 30.0%
GAACCAAAACAAGTTAGATA+AGG + chr1.4:67724242-67724261 None:intergenic 30.0%
GAATTCAAGTCTCATTATGT+TGG - chr1.4:67724651-67724670 MS.gene056092:CDS 30.0%
GACATGGAATTTCAGAAAAA+AGG - chr1.4:67723875-67723894 MS.gene056092:CDS 30.0%
GTGAATACCAGAATTTGTAT+TGG + chr1.4:67725452-67725471 None:intergenic 30.0%
GTTCCTTAATATGTTTAGGT+AGG - chr1.4:67724258-67724277 MS.gene056092:CDS 30.0%
TAAGAAAGATACATTCCCTT+TGG + chr1.4:67723576-67723595 None:intergenic 30.0%
TATAGCTTCTGATGGAATTT+GGG - chr1.4:67724514-67724533 MS.gene056092:CDS 30.0%
TGAGTAAGACTGAATTATCA+AGG - chr1.4:67723195-67723214 MS.gene056092:CDS 30.0%
TTAGTGAAATGTTTGATGAG+AGG - chr1.4:67723470-67723489 MS.gene056092:intron 30.0%
TTATAGCTTCTGATGGAATT+TGG - chr1.4:67724513-67724532 MS.gene056092:CDS 30.0%
!! AACTAGAGAAAGTGCATTTT+CGG + chr1.4:67725213-67725232 None:intergenic 30.0%
!! TTTGATGAGAGGTATTTGAT+TGG - chr1.4:67723481-67723500 MS.gene056092:intron 30.0%
!!! ATGTCCTTATTTTCCCATTT+TGG - chr1.4:67725360-67725379 MS.gene056092:intron 30.0%
!!! CTTCCTTATCTAACTTGTTT+TGG - chr1.4:67724236-67724255 MS.gene056092:CDS 30.0%
!!! TGTCCTTATTTTCCCATTTT+GGG - chr1.4:67725361-67725380 MS.gene056092:intron 30.0%
AAAGCTAGGAAACAAATCAG+GGG + chr1.4:67724472-67724491 None:intergenic 35.0%
ACACATGAATACACCCAAAA+TGG + chr1.4:67725377-67725396 None:intergenic 35.0%
ACACCCAAAATGGGAAAATA+AGG + chr1.4:67725367-67725386 None:intergenic 35.0%
ACTAATAAGCTTTCTGCAGT+TGG - chr1.4:67724879-67724898 MS.gene056092:intron 35.0%
ACTTGCTTAACATGTGGTAT+TGG + chr1.4:67724360-67724379 None:intergenic 35.0%
CACATGAATACACCCAAAAT+GGG + chr1.4:67725376-67725395 None:intergenic 35.0%
CGTTTGTGATTATTGATGGA+TGG - chr1.4:67724004-67724023 MS.gene056092:CDS 35.0%
GAAAGCTAGGAAACAAATCA+GGG + chr1.4:67724473-67724492 None:intergenic 35.0%
GCTACGTTTGTGATTATTGA+TGG - chr1.4:67724000-67724019 MS.gene056092:CDS 35.0%
GGAAGAAAATGTAGAAGAGA+GGG - chr1.4:67724116-67724135 MS.gene056092:intron 35.0%
GGATACTTTGTCTTCTGATA+CGG - chr1.4:67724535-67724554 MS.gene056092:intron 35.0%
GTTCCTTCCAATACAAATTC+TGG - chr1.4:67725442-67725461 MS.gene056092:CDS 35.0%
TACCGGAGTGATAAAATGAT+CGG + chr1.4:67725530-67725549 None:intergenic 35.0%
TCTCAAAACTCGACAAACAA+AGG - chr1.4:67724855-67724874 MS.gene056092:intron 35.0%
TGAAAGCTAGGAAACAAATC+AGG + chr1.4:67724474-67724493 None:intergenic 35.0%
TGAGCTACGAAATGGAAAAA+CGG - chr1.4:67723259-67723278 MS.gene056092:CDS 35.0%
TGGAAGAAAATGTAGAAGAG+AGG - chr1.4:67724115-67724134 MS.gene056092:intron 35.0%
! ACATTTTCTCAGTGATCAAG+TGG - chr1.4:67723645-67723664 MS.gene056092:intron 35.0%
! ATAAAATGATCGGTGTTCAC+TGG + chr1.4:67725520-67725539 None:intergenic 35.0%
! GTTAAGGTGATGGTTATTTG+TGG - chr1.4:67724608-67724627 MS.gene056092:intron 35.0%
! GTTTTCTTAAAATGCAGGCT+AGG - chr1.4:67725412-67725431 MS.gene056092:intron 35.0%
! TGTTTAGGGTCTGTTTACTT+CGG - chr1.4:67725050-67725069 MS.gene056092:CDS 35.0%
! TGTTTCCTAGCTTTCAAATG+CGG - chr1.4:67724478-67724497 MS.gene056092:CDS 35.0%
! TTCCAATCACTTGTTGATGA+TGG + chr1.4:67725024-67725043 None:intergenic 35.0%
! TTTTTCCATTTCGTAGCTCA+CGG + chr1.4:67723259-67723278 None:intergenic 35.0%
!! AACGTCATTTTCTGTTTTCG+CGG - chr1.4:67723382-67723401 MS.gene056092:intron 35.0%
!! TTCTAAAGTTCAATGCTCAC+TGG - chr1.4:67724444-67724463 MS.gene056092:CDS 35.0%
!!! GGCAGTGTTCTTATTTTTTG+AGG - chr1.4:67724629-67724648 MS.gene056092:CDS 35.0%
!!! GTGAGTTGGTTTCATTTTCA+TGG - chr1.4:67724384-67724403 MS.gene056092:intron 35.0%
AAAATGTAGAAGAGAGGGAG+CGG - chr1.4:67724121-67724140 MS.gene056092:intron 40.0%
AACGATATACTCTCCCACAT+CGG + chr1.4:67724337-67724356 None:intergenic 40.0%
AACTTGAACAGGAAGCTCTA+AGG - chr1.4:67724700-67724719 MS.gene056092:CDS 40.0%
ACCACATGTTAAGCAAGTGA+AGG - chr1.4:67724362-67724381 MS.gene056092:intron 40.0%
ACCTTCACTTGCTTAACATG+TGG + chr1.4:67724366-67724385 None:intergenic 40.0%
ATATACCATTATGCCCGTCA+AGG + chr1.4:67723730-67723749 None:intergenic 40.0%
CCTGGGTATCTAATATGCAT+CGG + chr1.4:67724059-67724078 None:intergenic 40.0%
CGATGCATATTAGATACCCA+GGG - chr1.4:67724057-67724076 MS.gene056092:CDS 40.0%
CTGGGTATCTAATATGCATC+GGG + chr1.4:67724058-67724077 None:intergenic 40.0%
CTGTCATTGAGAGAACATGA+AGG + chr1.4:67725238-67725257 None:intergenic 40.0%
CTTAAAATGCAGGCTAGGTA+AGG - chr1.4:67725417-67725436 MS.gene056092:intron 40.0%
GAACAAGTACAGCTCCAATA+AGG + chr1.4:67723924-67723943 None:intergenic 40.0%
GCGTCAATTTATTCCCACAA+TGG - chr1.4:67723669-67723688 MS.gene056092:intron 40.0%
GGCCCTACCTAAACATATTA+AGG + chr1.4:67724264-67724283 None:intergenic 40.0%
GTAGGAAGACTCAACGTATT+CGG - chr1.4:67724165-67724184 MS.gene056092:intron 40.0%
GTGCTTAGTTCACTTCTCTT+TGG - chr1.4:67724825-67724844 MS.gene056092:intron 40.0%
GTGGAGGAGTTATTTGAAGA+GGG - chr1.4:67724906-67724925 MS.gene056092:intron 40.0%
TGCCATCATCAACAAGTGAT+TGG - chr1.4:67725019-67725038 MS.gene056092:CDS 40.0%
TGTCACCAATCGATCTAGAA+AGG + chr1.4:67724314-67724333 None:intergenic 40.0%
TGTGGAGGAGTTATTTGAAG+AGG - chr1.4:67724905-67724924 MS.gene056092:intron 40.0%
TGTTTCTTTCTTGGAGTTGC+TGG + chr1.4:67724801-67724820 None:intergenic 40.0%
TTAAGCAAGTGAAGGTGAGT+TGG - chr1.4:67724370-67724389 MS.gene056092:intron 40.0%
TTCCTTCGCAAAAATAGCAG+CGG + chr1.4:67723753-67723772 None:intergenic 40.0%
TTGTGCCTTTCTAGATCGAT+TGG - chr1.4:67724306-67724325 MS.gene056092:CDS 40.0%
! CACCGATCATTTTATCACTC+CGG - chr1.4:67725525-67725544 MS.gene056092:CDS 40.0%
! CGCTTTTGTGAAAACTGACA+TGG - chr1.4:67723859-67723878 MS.gene056092:intron 40.0%
! CTAGTGAGCCTTTTATGTGA+AGG - chr1.4:67724414-67724433 MS.gene056092:intron 40.0%
! GAGAAGCCTTTTGTGAAGTA+TGG - chr1.4:67723281-67723300 MS.gene056092:CDS 40.0%
! GTTTAGGGTCTGTTTACTTC+GGG - chr1.4:67725051-67725070 MS.gene056092:CDS 40.0%
! GTTTCCTAGCTTTCAAATGC+GGG - chr1.4:67724479-67724498 MS.gene056092:CDS 40.0%
! TCATTTTCTGTTTTCGCGGT+AGG - chr1.4:67723386-67723405 MS.gene056092:intron 40.0%
! TTCTGCTTCTAGAGACCAAA+GGG - chr1.4:67723558-67723577 MS.gene056092:intron 40.0%
!!! TTACTTTTTACATCGGCAGC+TGG + chr1.4:67725312-67725331 None:intergenic 40.0%
AAATGTAGAAGAGAGGGAGC+GGG - chr1.4:67724122-67724141 MS.gene056092:intron 45.0%
AAGAAGAAAGACGCCATGGA+AGG - chr1.4:67725592-67725611 MS.gene056092:CDS 45.0%
AAGAATCGCCAGGAACTCTA+TGG + chr1.4:67723361-67723380 None:intergenic 45.0%
AAGACGCCATGGAAGGAAAA+AGG - chr1.4:67725599-67725618 MS.gene056092:CDS 45.0%
AATGACGTTGAAGAATCGCC+AGG + chr1.4:67723371-67723390 None:intergenic 45.0%
ACAGATTGCCATAGAGTTCC+TGG - chr1.4:67723350-67723369 MS.gene056092:CDS 45.0%
ACCGTTACAGTTGCATCTGT+TGG - chr1.4:67724027-67724046 MS.gene056092:CDS 45.0%
ACTGGTAAACCATCACCAGA+TGG + chr1.4:67725502-67725521 None:intergenic 45.0%
CCCAACAGATGCAACTGTAA+CGG + chr1.4:67724031-67724050 None:intergenic 45.0%
CCGATGCATATTAGATACCC+AGG - chr1.4:67724056-67724075 MS.gene056092:CDS 45.0%
CCGTTACAGTTGCATCTGTT+GGG - chr1.4:67724028-67724047 MS.gene056092:CDS 45.0%
CGTTACAGTTGCATCTGTTG+GGG - chr1.4:67724029-67724048 MS.gene056092:CDS 45.0%
CTTGACAGTTGATCACAGAC+TGG - chr1.4:67724095-67724114 MS.gene056092:intron 45.0%
CTTGATTGGCAGATCCTTGA+CGG - chr1.4:67723713-67723732 MS.gene056092:intron 45.0%
GCCTGTCCATACTTCACAAA+AGG + chr1.4:67723290-67723309 None:intergenic 45.0%
GTTCTGCAATGCTTACAGAG+AGG - chr1.4:67724928-67724947 MS.gene056092:CDS 45.0%
TCAGAAGAAGAAAGACGCCA+TGG - chr1.4:67725588-67725607 MS.gene056092:CDS 45.0%
TCTTCTGACAGTTTGTGCAG+AGG + chr1.4:67725576-67725595 None:intergenic 45.0%
TGACAGTTTGTGCAGAGGAA+TGG + chr1.4:67725571-67725590 None:intergenic 45.0%
TGCAACACAGGATAGTCTGA+AGG + chr1.4:67724774-67724793 None:intergenic 45.0%
TGCATATTAGATACCCAGGG+AGG - chr1.4:67724060-67724079 MS.gene056092:CDS 45.0%
TTGATTGGCAGATCCTTGAC+GGG - chr1.4:67723714-67723733 MS.gene056092:intron 45.0%
TTTGTGAAGTATGGACAGGC+TGG - chr1.4:67723290-67723309 MS.gene056092:CDS 45.0%
! GCCTTTTGTGAAGTATGGAC+AGG - chr1.4:67723286-67723305 MS.gene056092:CDS 45.0%
! GCTTTCTGCAGTTGGTATTG+TGG - chr1.4:67724887-67724906 MS.gene056092:intron 45.0%
! GTTCTGCTTCTAGAGACCAA+AGG - chr1.4:67723557-67723576 MS.gene056092:intron 45.0%
! TCAGGACCTTTTTCCTTCCA+TGG + chr1.4:67725608-67725627 None:intergenic 45.0%
! TTCTGCAGTTGGTATTGTGG+AGG - chr1.4:67724890-67724909 MS.gene056092:intron 45.0%
!! AGGAAGCTCTAAGGTCAAGA+GGG - chr1.4:67724709-67724728 MS.gene056092:CDS 45.0%
!! CAGGCTGGTTTGGCAAAAAA+AGG - chr1.4:67723305-67723324 MS.gene056092:CDS 45.0%
!!! ATCCGCTGCTATTTTTGCGA+AGG - chr1.4:67723748-67723767 MS.gene056092:intron 45.0%
AAGGTGTAAGCAGGGAGGAA+TGG - chr1.4:67723807-67723826 MS.gene056092:intron 50.0%
AAGGTTGCCAACGGAACAAG+TGG + chr1.4:67723230-67723249 None:intergenic 50.0%
AGAGAGAAACAACACCTCCC+TGG + chr1.4:67724077-67724096 None:intergenic 50.0%
AGGTACACCACGACATGACT+TGG + chr1.4:67724564-67724583 None:intergenic 50.0%
CAACGTATTCGGAGGCAATG+AGG - chr1.4:67724176-67724195 MS.gene056092:intron 50.0%
CAAGCTGGTGGTTAAGGTGA+TGG - chr1.4:67724598-67724617 MS.gene056092:intron 50.0%
CACCAGATGGTTGATCTGCT+AGG + chr1.4:67725489-67725508 None:intergenic 50.0%
CACTCCGGTATCTAAGCCAT+GGG - chr1.4:67725540-67725559 MS.gene056092:CDS 50.0%
CATACCGCAAGGTGTAAGCA+GGG - chr1.4:67723799-67723818 MS.gene056092:intron 50.0%
CATCACCTTAACCACCAGCT+TGG + chr1.4:67724600-67724619 None:intergenic 50.0%
CCTTCCCATGGCTTAGATAC+CGG + chr1.4:67725547-67725566 None:intergenic 50.0%
GAAGCAACCACTTGTTCCGT+TGG - chr1.4:67723220-67723239 MS.gene056092:CDS 50.0%
GAGAGAAACAACACCTCCCT+GGG + chr1.4:67724076-67724095 None:intergenic 50.0%
GCAGATCAACCATCTGGTGA+TGG - chr1.4:67725490-67725509 MS.gene056092:CDS 50.0%
GGAAGACTCAACGTATTCGG+AGG - chr1.4:67724168-67724187 MS.gene056092:intron 50.0%
GTTCCGTTGGCAACCTTGAT+CGG - chr1.4:67723233-67723252 MS.gene056092:CDS 50.0%
TCACTCCGGTATCTAAGCCA+TGG - chr1.4:67725539-67725558 MS.gene056092:CDS 50.0%
TCTTCCTACTTCACCACCAC+TGG + chr1.4:67724154-67724173 None:intergenic 50.0%
TGCCTAGCAGATCAACCATC+TGG - chr1.4:67725484-67725503 MS.gene056092:CDS 50.0%
TTACCTGCAGGGGAAACTTC+TGG - chr1.4:67723970-67723989 MS.gene056092:CDS 50.0%
! AATGGCTTCAAGCACTGCCT+CGG - chr1.4:67723825-67723844 MS.gene056092:intron 50.0%
! ATCGATTGGTGACACCGATG+TGG - chr1.4:67724320-67724339 MS.gene056092:intron 50.0%
! CAGATCCTTGACGGGCATAA+TGG - chr1.4:67723722-67723741 MS.gene056092:intron 50.0%
! CTAGAAAGGCACAATCCACC+AGG + chr1.4:67724300-67724319 None:intergenic 50.0%
! GAAGTATGGACAGGCTGGTT+TGG - chr1.4:67723295-67723314 MS.gene056092:CDS 50.0%
! TCCTAGCTTTCAAATGCGGG+CGG - chr1.4:67724482-67724501 MS.gene056092:CDS 50.0%
! TCGATTGGTGACACCGATGT+GGG - chr1.4:67724321-67724340 MS.gene056092:intron 50.0%
! TCTAAGGTCAAGAGGGCTGA+AGG - chr1.4:67724716-67724735 MS.gene056092:CDS 50.0%
!! CAGGAAGCTCTAAGGTCAAG+AGG - chr1.4:67724708-67724727 MS.gene056092:CDS 50.0%
AAAGCGACAACTAGAGCCCG+AGG + chr1.4:67723845-67723864 None:intergenic 55.0%
AGCTTGGCAGCAAGTTCTGC+AGG + chr1.4:67724584-67724603 None:intergenic 55.0%
ATCGGCCGTGAGCTACGAAA+TGG - chr1.4:67723251-67723270 MS.gene056092:CDS 55.0%
CAGAGGAATGGACCTTCCCA+TGG + chr1.4:67725559-67725578 None:intergenic 55.0%
CCATACCGCAAGGTGTAAGC+AGG - chr1.4:67723798-67723817 MS.gene056092:intron 55.0%
CCGGTATCTAAGCCATGGGA+AGG - chr1.4:67725544-67725563 MS.gene056092:CDS 55.0%
CCTGCTTACACCTTGCGGTA+TGG + chr1.4:67723801-67723820 None:intergenic 55.0%
CTGCTAGGCAAACTGCACAG+CGG + chr1.4:67725474-67725493 None:intergenic 55.0%
GTCATGAGTGCCATACCGCA+AGG - chr1.4:67723788-67723807 MS.gene056092:intron 55.0%
GTTCCAGAAGTTTCCCCTGC+AGG + chr1.4:67723976-67723995 None:intergenic 55.0%
GTTGCTGGCATCTGCAACAC+AGG + chr1.4:67724786-67724805 None:intergenic 55.0%
TCCGCCCGCATTTGAAAGCT+AGG + chr1.4:67724486-67724505 None:intergenic 55.0%
TCCTCCCTGCTTACACCTTG+CGG + chr1.4:67723806-67723825 None:intergenic 55.0%
TGCTGCCAAGCTGGTGGTTA+AGG - chr1.4:67724592-67724611 MS.gene056092:intron 55.0%
! ACAGCCAGTGGTGGTGAAGT+AGG - chr1.4:67724147-67724166 MS.gene056092:intron 55.0%
! ATGGCTTCAAGCACTGCCTC+GGG - chr1.4:67723826-67723845 MS.gene056092:intron 55.0%
!! AGAACTTGCTGCCAAGCTGG+TGG - chr1.4:67724586-67724605 MS.gene056092:intron 55.0%
!! TGCAGAACTTGCTGCCAAGC+TGG - chr1.4:67724583-67724602 MS.gene056092:intron 55.0%
ACCGCAAGGTGTAAGCAGGG+AGG - chr1.4:67723802-67723821 MS.gene056092:intron 60.0%
ACGGCTGCCAAGTCATGTCG+TGG - chr1.4:67724554-67724573 MS.gene056092:intron 60.0%
AGGTAGGGCCTCTTCGCTGT+TGG - chr1.4:67724274-67724293 MS.gene056092:CDS 60.0%
CGGCCGATCAAGGTTGCCAA+CGG + chr1.4:67723239-67723258 None:intergenic 60.0%
TCGTAGCTCACGGCCGATCA+AGG + chr1.4:67723249-67723268 None:intergenic 60.0%
CCACCAGGCCAACAGCGAAG+AGG + chr1.4:67724285-67724304 None:intergenic 65.0%
! CCTCTTCGCTGTTGGCCTGG+TGG - chr1.4:67724282-67724301 MS.gene056092:CDS 65.0%
!! AGGGAGCGGGTTACAGCCAG+TGG - chr1.4:67724135-67724154 MS.gene056092:intron 65.0%
!! GAGCGGGTTACAGCCAGTGG+TGG - chr1.4:67724138-67724157 MS.gene056092:intron 65.0%
GGGCCTCTTCGCTGTTGGCC+TGG - chr1.4:67724279-67724298 MS.gene056092:CDS 70.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 67723194 67725627 67723194 ID=MS.gene056092
chr1.4 mRNA 67723194 67725627 67723194 ID=MS.gene056092.t1;Parent=MS.gene056092
chr1.4 exon 67725418 67725627 67725418 ID=MS.gene056092.t1.exon1;Parent=MS.gene056092.t1
chr1.4 CDS 67725418 67725627 67725418 ID=cds.MS.gene056092.t1;Parent=MS.gene056092.t1
chr1.4 exon 67724925 67725096 67724925 ID=MS.gene056092.t1.exon2;Parent=MS.gene056092.t1
chr1.4 CDS 67724925 67725096 67724925 ID=cds.MS.gene056092.t1;Parent=MS.gene056092.t1
chr1.4 exon 67724624 67724841 67724624 ID=MS.gene056092.t1.exon3;Parent=MS.gene056092.t1
chr1.4 CDS 67724624 67724841 67724624 ID=cds.MS.gene056092.t1;Parent=MS.gene056092.t1
chr1.4 exon 67724438 67724545 67724438 ID=MS.gene056092.t1.exon4;Parent=MS.gene056092.t1
chr1.4 CDS 67724438 67724545 67724438 ID=cds.MS.gene056092.t1;Parent=MS.gene056092.t1
chr1.4 exon 67724208 67724333 67724208 ID=MS.gene056092.t1.exon5;Parent=MS.gene056092.t1
chr1.4 CDS 67724208 67724333 67724208 ID=cds.MS.gene056092.t1;Parent=MS.gene056092.t1
chr1.4 exon 67723872 67724110 67723872 ID=MS.gene056092.t1.exon6;Parent=MS.gene056092.t1
chr1.4 CDS 67723872 67724110 67723872 ID=cds.MS.gene056092.t1;Parent=MS.gene056092.t1
chr1.4 exon 67723194 67723392 67723194 ID=MS.gene056092.t1.exon7;Parent=MS.gene056092.t1
chr1.4 CDS 67723194 67723392 67723194 ID=cds.MS.gene056092.t1;Parent=MS.gene056092.t1
Gene Sequence

>MS.gene056092

ATGAGTAAGACTGAATTATCAAGGATGAAGCAACCACTTGTTCCGTTGGCAACCTTGATCGGCCGTGAGCTACGAAATGGAAAAACGGAGAAGCCTTTTGTGAAGTATGGACAGGCTGGTTTGGCAAAAAAAGGAGAAGATTACTTTCTAATCAAGACAGATTGCCATAGAGTTCCTGGCGATTCTTCAACGTCATTTTCTGTTTTCGCGGTAGGTATCATACACGTTCTTTGTTTACATGTGTTTTTAATTAACTTGATTGTCATTCTTTTGTTTTTAGTGAAATGTTTGATGAGAGGTATTTGATTGGTAAGAAATGAAAATATTGCTTAAGATAAGATCTTATGAATGAATTGGCAATTTGTTCTGCTTCTAGAGACCAAAGGGAATGTATCTTTCTTAGGTTTAGTACATAAATGAGAGTTGAAGTGTGTGATTTCTATCTTATCTTACATTTTCTCAGTGATCAAGTGGAGCGTCAATTTATTCCCACAATGGTTATGATATCAATAAGGAATTCTTGATTGGCAGATCCTTGACGGGCATAATGGTATATCCGCTGCTATTTTTGCGAAGGAAAACATAATAAACAATGTCATGAGTGCCATACCGCAAGGTGTAAGCAGGGAGGAATGGCTTCAAGCACTGCCTCGGGCTCTAGTTGTCGCTTTTGTGAAAACTGACATGGAATTTCAGAAAAAAGGTACACACACAGTTTTCTTTGTATCCTTATTGGAGCTGTACTTGTTCATGTACACTCATTCTAAAATTTAATTTTACCTGCAGGGGAAACTTCTGGAACGACAGCTACGTTTGTGATTATTGATGGATGGACCGTTACAGTTGCATCTGTTGGGGATTCCCGATGCATATTAGATACCCAGGGAGGTGTTGTTTCTCTCTTGACAGTTGATCACAGACTGGAAGAAAATGTAGAAGAGAGGGAGCGGGTTACAGCCAGTGGTGGTGAAGTAGGAAGACTCAACGTATTCGGAGGCAATGAGGTATGATGTGTAATCTTCATATTCCTTTCATATAATTTCTTCCTTATCTAACTTGTTTTGGTTCCTTAATATGTTTAGGTAGGGCCTCTTCGCTGTTGGCCTGGTGGATTGTGCCTTTCTAGATCGATTGGTGACACCGATGTGGGAGAGTATATCGTTCCAATACCACATGTTAAGCAAGTGAAGGTGAGTTGGTTTCATTTTCATGGTTTTAGTCTAGTGAGCCTTTTATGTGAAGGTGTTTACTTCTAAAGTTCAATGCTCACTGGCTCCCCTGATTTGTTTCCTAGCTTTCAAATGCGGGCGGAAGACTAATTATAGCTTCTGATGGAATTTGGGATACTTTGTCTTCTGATACGGCTGCCAAGTCATGTCGTGGTGTACCTGCAGAACTTGCTGCCAAGCTGGTGGTTAAGGTGATGGTTATTTGTGGCAGTGTTCTTATTTTTTGAGGAATTCAAGTCTCATTATGTTGGTTACTCAATGCTTTGTCTATTGTTTTAACTTGAACAGGAAGCTCTAAGGTCAAGAGGGCTGAAGGATGATACAACCTGCCTTGTTGTAGATATTATTCCTTCAGACTATCCTGTGTTGCAGATGCCAGCAACTCCAAGAAAGAAACATAATGTGCTTAGTTCACTTCTCTTTGGAAAGAAATCTCAAAACTCGACAAACAAAGGAACTAATAAGCTTTCTGCAGTTGGTATTGTGGAGGAGTTATTTGAAGAGGGTTCTGCAATGCTTACAGAGAGGTCACTCTTAGACTATAACTGCAAAATTTATATTTGCATGCTGCCTTTTAAAGTTTAGTAGTTCTTTTGTGCCATCATCAACAAGTGATTGGAACAAAAATGTTTAGGGTCTGTTTACTTCGGGTGTTTTCTGTTTTTCATTTTAACTGATAAAATAAATATTAGAGAGACATTATAGACATTTTTGAAAATGCATTTTATAAATGGATTCAATATTTCTGAAAGTTCTGAAAAATGTCAAAACATTGTTGTTAGTGTTTCCGAAAATGCACTTTCTCTAGTTTGCCTTCATGTTCTCTCAATGACAGCCAAAAAGATTCATAATCAAATGTCATAAAGATTCATAATCAAATGTCATTCCAGCTGCCGATGTAAAAAGTAAAAATTGAAAATGTTTTTAGAAAGTAAACATGTCCTTATTTTCCCATTTTGGGTGTATTCATGTGTTTGATAAGATTTTTTGTTTTCTTAAAATGCAGGCTAGGTAAGGATGTTCCTTCCAATACAAATTCTGGTATTCACCGCTGTGCAGTTTGCCTAGCAGATCAACCATCTGGTGATGGTTTACCAGTGAACACCGATCATTTTATCACTCCGGTATCTAAGCCATGGGAAGGTCCATTCCTCTGCACAAACTGTCAGAAGAAGAAAGACGCCATGGAAGGAAAAAGGTCCTGA
Protein sequence

>MS.gene056092.t1

MSKTELSRMKQPLVPLATLIGRELRNGKTEKPFVKYGQAGLAKKGEDYFLIKTDCHRVPGDSSTSFSVFAILDGHNGISAAIFAKENIINNVMSAIPQGVSREEWLQALPRALVVAFVKTDMEFQKKGETSGTTATFVIIDGWTVTVASVGDSRCILDTQGGVVSLLTVDHRLEENVEERERVTASGGEVGRLNVFGGNEVGPLRCWPGGLCLSRSIGDTDVGEYIVPIPHVKQVKLSNAGGRLIIASDGIWDTLSSDTAAKSCRGVPAELAAKLVVKEALRSRGLKDDTTCLVVDIIPSDYPVLQMPATPRKKHNVLSSLLFGKKSQNSTNKGTNKLSAVGIVEELFEEGSAMLTERLGKDVPSNTNSGIHRCAVCLADQPSGDGLPVNTDHFITPVSKPWEGPFLCTNCQKKKDAMEGKRS