Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37713.t1 | XP_013452841.1 | 92.1 | 165 | 13 | 0 | 1 | 165 | 1 | 165 | 5.90E-79 | 303.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37713.t1 | D9UBX6 | 38.5 | 91 | 55 | 1 | 14 | 103 | 16 | 106 | 3.4e-11 | 69.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37713.t1 | A0A072UAS6 | 92.1 | 165 | 13 | 0 | 1 | 165 | 1 | 165 | 4.2e-79 | 303.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050807 | MS.gene37713 | 0.800711 | 1.27E-48 | -1.69E-46 |
| MS.gene051247 | MS.gene37713 | 0.804768 | 1.84E-49 | -1.69E-46 |
| MS.gene055466 | MS.gene37713 | 0.808267 | 3.37E-50 | -1.69E-46 |
| MS.gene058339 | MS.gene37713 | 0.815246 | 1.02E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37713.t1 | MTR_6g478190 | 92.121 | 165 | 13 | 0 | 1 | 165 | 1 | 165 | 1.47e-111 | 314 |
| MS.gene37713.t1 | MTR_7g086130 | 41.573 | 89 | 52 | 0 | 12 | 100 | 9 | 97 | 8.82e-21 | 84.7 |
| MS.gene37713.t1 | MTR_6g079910 | 89.474 | 38 | 4 | 0 | 1 | 38 | 1 | 38 | 1.83e-18 | 76.3 |
| MS.gene37713.t1 | MTR_7g086130 | 39.362 | 94 | 52 | 1 | 12 | 100 | 9 | 102 | 2.72e-18 | 78.6 |
| MS.gene37713.t1 | MTR_5g087470 | 43.158 | 95 | 54 | 0 | 6 | 100 | 5 | 99 | 2.86e-18 | 79.7 |
| MS.gene37713.t1 | MTR_5g087450 | 41.573 | 89 | 52 | 0 | 12 | 100 | 12 | 100 | 1.04e-17 | 77.8 |
| MS.gene37713.t1 | MTR_5g087480 | 31.298 | 131 | 82 | 3 | 1 | 126 | 1 | 128 | 9.15e-14 | 67.8 |
| MS.gene37713.t1 | MTR_5g087480 | 34.375 | 96 | 60 | 2 | 1 | 96 | 1 | 93 | 7.70e-13 | 65.1 |
| MS.gene37713.t1 | MTR_4g107860 | 31.818 | 110 | 72 | 1 | 15 | 124 | 23 | 129 | 2.63e-12 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37713.t1 | AT1G54400 | 43.750 | 96 | 53 | 1 | 7 | 101 | 6 | 101 | 7.51e-22 | 87.0 |
| MS.gene37713.t1 | AT2G27140 | 39.423 | 104 | 63 | 0 | 1 | 104 | 1 | 104 | 9.43e-20 | 82.8 |
| MS.gene37713.t1 | AT5G20970 | 39.130 | 115 | 67 | 2 | 12 | 124 | 8 | 121 | 3.14e-17 | 76.6 |
| MS.gene37713.t1 | AT5G04890 | 39.773 | 88 | 52 | 1 | 14 | 100 | 16 | 103 | 6.07e-15 | 71.2 |
Find 24 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCATAGAGAAACATTCATTT+TGG | 0.198351 | 6.3:-71256591 | MS.gene37713:CDS |
| TTTCTCTATGGTTGCATCTT+TGG | 0.207665 | 6.3:+71256603 | None:intergenic |
| CCAAAATGAATGTTTCTCTA+TGG | 0.325882 | 6.3:+71256591 | None:intergenic |
| AAAATCTTCATACAATCTTT+TGG | 0.330843 | 6.3:+71257508 | None:intergenic |
| TCCTGATTTCTTTGTGGAAA+CGG | 0.353868 | 6.3:+71256719 | None:intergenic |
| TTGAAATCCTGATTTCTTTG+TGG | 0.360661 | 6.3:+71256713 | None:intergenic |
| ACAACAATTCAAATTGTGAT+TGG | 0.374136 | 6.3:-71256547 | MS.gene37713:CDS |
| AAGGTTCAAACTAACGATAA+AGG | 0.388478 | 6.3:-71256790 | MS.gene37713:CDS |
| GCCGTTTCCACAAAGAAATC+AGG | 0.392632 | 6.3:-71256720 | MS.gene37713:CDS |
| AAACAACATCATGATGCATT+TGG | 0.402983 | 6.3:-71256472 | MS.gene37713:CDS |
| TCAAGAGATTGTAATGTGAA+TGG | 0.408463 | 6.3:-71256694 | MS.gene37713:CDS |
| CAAGAGATTGTAATGTGAAT+GGG | 0.410432 | 6.3:-71256693 | MS.gene37713:CDS |
| TCAAATTGTGATTGGAGTAA+TGG | 0.419262 | 6.3:-71256539 | MS.gene37713:CDS |
| GGTAGTAGCTTCAGCAACTT+TGG | 0.424641 | 6.3:+71257529 | None:intergenic |
| TTTGTGATCCATTTGCAGTA+AGG | 0.441165 | 6.3:+71257482 | None:intergenic |
| AGGTTCAAACTAACGATAAA+GGG | 0.452103 | 6.3:-71256789 | MS.gene37713:CDS |
| ATTTATGGAGAAAGGACTCT+TGG | 0.477919 | 6.3:-71256757 | MS.gene37713:CDS |
| ATTCTAGAGATTGATTTGAA+AGG | 0.547773 | 6.3:-71257446 | MS.gene37713:intron |
| GTAATGGCTATGGTTGCACT+AGG | 0.553548 | 6.3:-71256523 | MS.gene37713:CDS |
| TGTGATTGGAGTAATGGCTA+TGG | 0.602709 | 6.3:-71256533 | MS.gene37713:CDS |
| TGCAAATGGATCACAAAGGA+AGG | 0.605441 | 6.3:-71257476 | MS.gene37713:CDS |
| GGTTGCACTAGGGACTTATG+TGG | 0.633161 | 6.3:-71256512 | MS.gene37713:CDS |
| TAATGGCTATGGTTGCACTA+GGG | 0.679191 | 6.3:-71256522 | MS.gene37713:CDS |
| TTACTGCAAATGGATCACAA+AGG | 0.775712 | 6.3:-71257480 | MS.gene37713:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATTTATTAAAATCTGTA+AGG | + | chr6.3:71256741-71256760 | None:intergenic | 10.0% |
| !! | AAATTTATTAAAATCTGTAA+GGG | + | chr6.3:71256740-71256759 | None:intergenic | 10.0% |
| !! | ACTAATATTTGTTTATTAAA+TGG | + | chr6.3:71257116-71257135 | None:intergenic | 10.0% |
| !! | TATAATATTTAGTAAAATAG+AGG | + | chr6.3:71256803-71256822 | None:intergenic | 10.0% |
| !!! | CTATTTTACTAAATATTATA+AGG | - | chr6.3:71256803-71256822 | MS.gene37713:CDS | 10.0% |
| !! | AATTAGACAAAATAATTGTT+TGG | + | chr6.3:71256935-71256954 | None:intergenic | 15.0% |
| !! | AGACAAAAAAAAATTATAGT+TGG | - | chr6.3:71256690-71256709 | MS.gene37713:CDS | 15.0% |
| !! | GACAAAAAAAAATTATAGTT+GGG | - | chr6.3:71256691-71256710 | MS.gene37713:CDS | 15.0% |
| !!! | ATAAACAAATATTAGTCTAA+TGG | - | chr6.3:71257120-71257139 | MS.gene37713:intron | 15.0% |
| !!! | ATTTCTAACGTTTTTTATTT+TGG | + | chr6.3:71257062-71257081 | None:intergenic | 15.0% |
| !!! | GAATTTGAAACATTTTTTTT+AGG | - | chr6.3:71256830-71256849 | MS.gene37713:intron | 15.0% |
| !!! | TACGAAATTTATATTTTAAG+AGG | - | chr6.3:71256606-71256625 | MS.gene37713:CDS | 15.0% |
| !!! | TTTCTAACGTTTTTTATTTT+GGG | + | chr6.3:71257061-71257080 | None:intergenic | 15.0% |
| !! | AAAAATTGGGTAATTATTAG+AGG | + | chr6.3:71257024-71257043 | None:intergenic | 20.0% |
| !! | AAAATTATAGTTGGGTTATT+TGG | - | chr6.3:71256699-71256718 | MS.gene37713:CDS | 20.0% |
| !!! | AAAATCTTCATACAATCTTT+TGG | + | chr6.3:71256478-71256497 | None:intergenic | 20.0% |
| !!! | AATCATTTTAGGTTTCAAAA+AGG | - | chr6.3:71257162-71257181 | MS.gene37713:intron | 20.0% |
| !!! | TAGTAAAATAGAGGATTTTA+TGG | + | chr6.3:71256794-71256813 | None:intergenic | 20.0% |
| !!! | TATTTCTCTTGAATCATTTT+AGG | - | chr6.3:71257151-71257170 | MS.gene37713:intron | 20.0% |
| ! | AAAGGGATCCTAAAAATTTA+TGG | - | chr6.3:71257211-71257230 | MS.gene37713:intron | 25.0% |
| ! | ACAACAATTCAAATTGTGAT+TGG | - | chr6.3:71257436-71257455 | MS.gene37713:intron | 25.0% |
| ! | TCCTAAAAATTTATGGAGAA+AGG | - | chr6.3:71257218-71257237 | MS.gene37713:intron | 25.0% |
| ! | TTTCTCGACAACAAAAAATT+GGG | + | chr6.3:71257037-71257056 | None:intergenic | 25.0% |
| !! | AGGATTTTATGGTAAAAAAG+AGG | + | chr6.3:71256783-71256802 | None:intergenic | 25.0% |
| !! | ATAGAGAAACATTCATTTTG+GGG | - | chr6.3:71257394-71257413 | MS.gene37713:intron | 25.0% |
| !! | CATAGAGAAACATTCATTTT+GGG | - | chr6.3:71257393-71257412 | MS.gene37713:intron | 25.0% |
| !! | CTAACAAGAGTATATAATCA+AGG | + | chr6.3:71256573-71256592 | None:intergenic | 25.0% |
| !! | TCCTTTCTCCATAAATTTTT+AGG | + | chr6.3:71257222-71257241 | None:intergenic | 25.0% |
| !! | TGTTGAATTACTTCAGTTTT+TGG | + | chr6.3:71257358-71257377 | None:intergenic | 25.0% |
| !! | TTTTCTCGACAACAAAAAAT+TGG | + | chr6.3:71257038-71257057 | None:intergenic | 25.0% |
| !!! | ATTCTAGAGATTGATTTGAA+AGG | - | chr6.3:71256537-71256556 | MS.gene37713:CDS | 25.0% |
| !!! | TTTTGAGAAGAAAAGATAGT+GGG | + | chr6.3:71256870-71256889 | None:intergenic | 25.0% |
| !!! | TTTTTGAGAAGAAAAGATAG+TGG | + | chr6.3:71256871-71256890 | None:intergenic | 25.0% |
| !!! | TTTTTGTCTAACTTGCATAA+AGG | + | chr6.3:71256679-71256698 | None:intergenic | 25.0% |
| AAAATCAGAGACATGGTATT+GGG | - | chr6.3:71256644-71256663 | MS.gene37713:CDS | 30.0% | |
| AAACAACATCATGATGCATT+TGG | - | chr6.3:71257511-71257530 | MS.gene37713:CDS | 30.0% | |
| AAGGTTCAAACTAACGATAA+AGG | - | chr6.3:71257193-71257212 | MS.gene37713:intron | 30.0% | |
| AGGTTCAAACTAACGATAAA+GGG | - | chr6.3:71257194-71257213 | MS.gene37713:intron | 30.0% | |
| CAAGAGATTGTAATGTGAAT+GGG | - | chr6.3:71257290-71257309 | MS.gene37713:intron | 30.0% | |
| CCAAAATGAATGTTTCTCTA+TGG | + | chr6.3:71257395-71257414 | None:intergenic | 30.0% | |
| TAAAATCAGAGACATGGTAT+TGG | - | chr6.3:71256643-71256662 | MS.gene37713:CDS | 30.0% | |
| TCAAATTGTGATTGGAGTAA+TGG | - | chr6.3:71257444-71257463 | MS.gene37713:intron | 30.0% | |
| TCAAGAGATTGTAATGTGAA+TGG | - | chr6.3:71257289-71257308 | MS.gene37713:intron | 30.0% | |
| TTGAAATCCTGATTTCTTTG+TGG | + | chr6.3:71257273-71257292 | None:intergenic | 30.0% | |
| TTGGGTTATTTGGACAAAAA+GGG | - | chr6.3:71256709-71256728 | MS.gene37713:CDS | 30.0% | |
| TTTCAAAAAGGAGCAACTTA+AGG | - | chr6.3:71257174-71257193 | MS.gene37713:intron | 30.0% | |
| TTTGAGAAGAAAAGATAGTG+GGG | + | chr6.3:71256869-71256888 | None:intergenic | 30.0% | |
| ! | AATACCATGTCTCTGATTTT+AGG | + | chr6.3:71256644-71256663 | None:intergenic | 30.0% |
| ! | ATACCATGTCTCTGATTTTA+GGG | + | chr6.3:71256643-71256662 | None:intergenic | 30.0% |
| ! | ATTCAAGCAAAGTTTTCTCA+AGG | - | chr6.3:71257313-71257332 | MS.gene37713:intron | 30.0% |
| ! | ATTTTGAACCTTACTGCAAA+TGG | - | chr6.3:71256493-71256512 | MS.gene37713:CDS | 30.0% |
| ! | CCATAGAGAAACATTCATTT+TGG | - | chr6.3:71257392-71257411 | MS.gene37713:intron | 30.0% |
| ! | TAACTTGCATAAAGGACTAA+AGG | + | chr6.3:71256671-71256690 | None:intergenic | 30.0% |
| ! | TAGAGAAACATTCATTTTGG+GGG | - | chr6.3:71257395-71257414 | MS.gene37713:intron | 30.0% |
| ! | TTCAAGCAAAGTTTTCTCAA+GGG | - | chr6.3:71257314-71257333 | MS.gene37713:intron | 30.0% |
| ATTTATGGAGAAAGGACTCT+TGG | - | chr6.3:71257226-71257245 | MS.gene37713:intron | 35.0% | |
| GTTGGGTTATTTGGACAAAA+AGG | - | chr6.3:71256708-71256727 | MS.gene37713:CDS | 35.0% | |
| TATCCCTAAAATCAGAGACA+TGG | - | chr6.3:71256637-71256656 | MS.gene37713:CDS | 35.0% | |
| TCCTGATTTCTTTGTGGAAA+CGG | + | chr6.3:71257267-71257286 | None:intergenic | 35.0% | |
| TGGGTTATTTGGACAAAAAG+GGG | - | chr6.3:71256710-71256729 | MS.gene37713:CDS | 35.0% | |
| TTACTGCAAATGGATCACAA+AGG | - | chr6.3:71256503-71256522 | MS.gene37713:CDS | 35.0% | |
| TTTCTCTATGGTTGCATCTT+TGG | + | chr6.3:71257383-71257402 | None:intergenic | 35.0% | |
| TTTGTGATCCATTTGCAGTA+AGG | + | chr6.3:71256504-71256523 | None:intergenic | 35.0% | |
| ! | AGAGAAACATTCATTTTGGG+GGG | - | chr6.3:71257396-71257415 | MS.gene37713:intron | 35.0% |
| !!! | GATAGTGGGGCATTTTAATA+CGG | + | chr6.3:71256856-71256875 | None:intergenic | 35.0% |
| GTTATTTGGACAAAAAGGGG+TGG | - | chr6.3:71256713-71256732 | MS.gene37713:CDS | 40.0% | |
| TAATGGCTATGGTTGCACTA+GGG | - | chr6.3:71257461-71257480 | MS.gene37713:CDS | 40.0% | |
| TGCAAATGGATCACAAAGGA+AGG | - | chr6.3:71256507-71256526 | MS.gene37713:CDS | 40.0% | |
| TGTGATTGGAGTAATGGCTA+TGG | - | chr6.3:71257450-71257469 | MS.gene37713:CDS | 40.0% | |
| GCCGTTTCCACAAAGAAATC+AGG | - | chr6.3:71257263-71257282 | MS.gene37713:intron | 45.0% | |
| GGTAGTAGCTTCAGCAACTT+TGG | + | chr6.3:71256457-71256476 | None:intergenic | 45.0% | |
| GTAATGGCTATGGTTGCACT+AGG | - | chr6.3:71257460-71257479 | MS.gene37713:CDS | 45.0% | |
| !!! | TATTTTACTAAATATTATAA+GGG | - | chr6.3:71256804-71256823 | MS.gene37713:CDS | 5.0% |
| GGTTGCACTAGGGACTTATG+TGG | - | chr6.3:71257471-71257490 | MS.gene37713:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 71256447 | 71257558 | 71256447 | ID=MS.gene37713 |
| chr6.3 | mRNA | 71256447 | 71257558 | 71256447 | ID=MS.gene37713.t1;Parent=MS.gene37713 |
| chr6.3 | exon | 71257447 | 71257558 | 71257447 | ID=MS.gene37713.t1.exon1;Parent=MS.gene37713.t1 |
| chr6.3 | CDS | 71257447 | 71257558 | 71257447 | ID=cds.MS.gene37713.t1;Parent=MS.gene37713.t1 |
| chr6.3 | exon | 71256447 | 71256832 | 71256447 | ID=MS.gene37713.t1.exon2;Parent=MS.gene37713.t1 |
| chr6.3 | CDS | 71256447 | 71256832 | 71256447 | ID=cds.MS.gene37713.t1;Parent=MS.gene37713.t1 |
| Gene Sequence |
| Protein sequence |
