Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37737.t1 | XP_003620175.1 | 94.9 | 214 | 10 | 1 | 1 | 214 | 1 | 213 | 1.10E-114 | 422.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37737.t1 | P13087 | 44.2 | 215 | 106 | 5 | 6 | 213 | 9 | 216 | 2.4e-41 | 170.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37737.t1 | Q6ISX8 | 94.9 | 214 | 10 | 1 | 1 | 214 | 1 | 213 | 8.1e-115 | 422.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05227 | MS.gene37737 | 0.851432 | 8.91E-61 | -1.69E-46 |
| MS.gene05228 | MS.gene37737 | 0.916335 | 2.01E-85 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37737.t1 | MTR_6g078140 | 94.860 | 214 | 10 | 1 | 1 | 214 | 1 | 213 | 1.34e-136 | 381 |
| MS.gene37737.t1 | MTR_6g078120 | 94.393 | 214 | 11 | 1 | 1 | 214 | 1 | 213 | 1.95e-135 | 378 |
| MS.gene37737.t1 | MTR_6g078280 | 62.000 | 200 | 69 | 4 | 18 | 214 | 17 | 212 | 1.69e-82 | 244 |
| MS.gene37737.t1 | MTR_6g078290 | 58.605 | 215 | 85 | 4 | 1 | 214 | 1 | 212 | 9.32e-81 | 240 |
| MS.gene37737.t1 | MTR_6g078250 | 56.633 | 196 | 76 | 2 | 16 | 211 | 15 | 201 | 9.20e-73 | 219 |
| MS.gene37737.t1 | MTR_6g078260 | 48.165 | 218 | 90 | 6 | 1 | 214 | 1 | 199 | 3.57e-58 | 182 |
| MS.gene37737.t1 | MTR_7g037410 | 48.663 | 187 | 84 | 4 | 29 | 214 | 27 | 202 | 1.32e-55 | 176 |
| MS.gene37737.t1 | MTR_6g078070 | 40.359 | 223 | 104 | 10 | 1 | 214 | 1 | 203 | 1.04e-39 | 135 |
| MS.gene37737.t1 | MTR_6g078100 | 40.265 | 226 | 102 | 11 | 1 | 214 | 1 | 205 | 1.08e-38 | 132 |
| MS.gene37737.t1 | MTR_6g065460 | 31.019 | 216 | 135 | 5 | 1 | 214 | 1 | 204 | 8.53e-21 | 86.7 |
| MS.gene37737.t1 | MTR_3g106620 | 29.545 | 220 | 123 | 7 | 6 | 214 | 1 | 199 | 4.40e-20 | 84.7 |
| MS.gene37737.t1 | MTR_3g106585 | 31.390 | 223 | 113 | 9 | 6 | 214 | 1 | 197 | 1.11e-18 | 80.9 |
| MS.gene37737.t1 | MTR_2g073010 | 33.036 | 224 | 114 | 11 | 1 | 214 | 1 | 198 | 5.88e-17 | 76.3 |
| MS.gene37737.t1 | MTR_8g461050 | 29.384 | 211 | 127 | 8 | 10 | 213 | 6 | 201 | 6.06e-15 | 70.9 |
| MS.gene37737.t1 | MTR_6g017225 | 32.530 | 166 | 96 | 6 | 10 | 174 | 12 | 162 | 1.73e-14 | 71.6 |
| MS.gene37737.t1 | MTR_6g009563 | 30.698 | 215 | 123 | 9 | 6 | 214 | 9 | 203 | 1.77e-13 | 67.0 |
| MS.gene37737.t1 | MTR_6g059410 | 29.412 | 221 | 122 | 11 | 1 | 214 | 1 | 194 | 7.22e-13 | 65.1 |
| MS.gene37737.t1 | MTR_8g059790 | 28.947 | 190 | 113 | 8 | 31 | 213 | 29 | 203 | 1.29e-12 | 64.7 |
| MS.gene37737.t1 | MTR_6g059530 | 29.412 | 221 | 123 | 10 | 1 | 214 | 1 | 195 | 1.74e-12 | 64.3 |
| MS.gene37737.t1 | MTR_6g009650 | 31.707 | 205 | 114 | 9 | 6 | 205 | 9 | 192 | 2.13e-12 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37737.t1 | AT1G17860 | 43.119 | 218 | 97 | 8 | 1 | 214 | 1 | 195 | 9.08e-48 | 155 |
| MS.gene37737.t1 | AT1G73260 | 36.500 | 200 | 109 | 6 | 21 | 214 | 18 | 205 | 2.30e-29 | 109 |
| MS.gene37737.t1 | AT1G73325 | 34.091 | 220 | 122 | 9 | 1 | 214 | 1 | 203 | 1.37e-26 | 102 |
| MS.gene37737.t1 | AT1G73330 | 27.979 | 193 | 108 | 8 | 28 | 214 | 25 | 192 | 1.73e-11 | 61.6 |
Find 48 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATCTTGAACCAATTATTAA+TGG | 0.190104 | 6.3:-72068285 | None:intergenic |
| TTGAGCACACAACCACTTCT+TGG | 0.247520 | 6.3:+72067864 | MS.gene37737:CDS |
| CTTGTTGAAATTTAACTTTA+AGG | 0.254449 | 6.3:-72068439 | None:intergenic |
| GATGGGAAACCATTAATAAT+TGG | 0.274442 | 6.3:+72068276 | MS.gene37737:CDS |
| GGAGTGTTTGTTGATGAAAA+TGG | 0.277827 | 6.3:+72068386 | MS.gene37737:CDS |
| CACCTGTGAATGGGTATAAC+CGG | 0.336556 | 6.3:-72067966 | None:intergenic |
| AGACAATGGTTTATTACTAT+TGG | 0.339490 | 6.3:+72068233 | MS.gene37737:CDS |
| TTGTTGAAATTTAACTTTAA+GGG | 0.352360 | 6.3:-72068438 | None:intergenic |
| ATTCACAGGTGTGGACCTTA+TGG | 0.370571 | 6.3:+72067978 | MS.gene37737:CDS |
| TGGTTCAAGATTGAGAAGTA+TGG | 0.396705 | 6.3:+72068296 | MS.gene37737:CDS |
| TGGTGGTGTTGTTGGAAATC+CGG | 0.404788 | 6.3:+72068253 | MS.gene37737:CDS |
| GAGTGTTTGTTGATGAAAAT+GGG | 0.410318 | 6.3:+72068387 | MS.gene37737:CDS |
| TGAAAATGGGAATAAGCGTT+TGG | 0.412010 | 6.3:+72068400 | MS.gene37737:CDS |
| ATTACTATTGGTGGTGTTGT+TGG | 0.423355 | 6.3:+72068245 | MS.gene37737:CDS |
| GAACAAGTGGTTGACACATT+AGG | 0.434007 | 6.3:+72067906 | MS.gene37737:CDS |
| CTACTTCTACATTTACCATA+AGG | 0.458135 | 6.3:-72067993 | None:intergenic |
| TTGAAAGACTGCACCACACT+AGG | 0.459638 | 6.3:-72068344 | None:intergenic |
| AGTCTTGCCCTAGCAAGTAT+TGG | 0.463122 | 6.3:+72068023 | MS.gene37737:CDS |
| GTCTTTCAAGCATATGTGTA+AGG | 0.466167 | 6.3:+72068358 | MS.gene37737:CDS |
| AACTTGCCCTCTTGATGTTG+TGG | 0.473079 | 6.3:+72068049 | MS.gene37737:CDS |
| CAATGGTTTATTACTATTGG+TGG | 0.475905 | 6.3:+72068236 | MS.gene37737:CDS |
| TTATTAATGGTTTCCCATCC+CGG | 0.501459 | 6.3:-72068272 | None:intergenic |
| AAGCATATGTGTAAGGATGT+TGG | 0.504444 | 6.3:+72068365 | MS.gene37737:CDS |
| ATAAGGTCCACACCTGTGAA+TGG | 0.514083 | 6.3:-72067976 | None:intergenic |
| TAAGGTCCACACCTGTGAAT+GGG | 0.519817 | 6.3:-72067975 | None:intergenic |
| TTAACGGGTGTAAAGGTTAG+TGG | 0.525966 | 6.3:-72068095 | None:intergenic |
| TGTTGAAATTTAACTTTAAG+GGG | 0.534245 | 6.3:-72068437 | None:intergenic |
| TGCTAATTACTATATTATCC+CGG | 0.540548 | 6.3:+72067947 | MS.gene37737:CDS |
| GGTGGTGTTGTTGGAAATCC+GGG | 0.542963 | 6.3:+72068254 | MS.gene37737:CDS |
| GCTTCAGCTGCTCCAAGAAG+TGG | 0.551993 | 6.3:-72067876 | None:intergenic |
| TCCCGGTTATACCCATTCAC+AGG | 0.554778 | 6.3:+72067964 | MS.gene37737:CDS |
| GTGTTGTTGGAAATCCGGGA+TGG | 0.564391 | 6.3:+72068258 | MS.gene37737:CDS |
| TGTTGTTGGAAATCCGGGAT+GGG | 0.569448 | 6.3:+72068259 | MS.gene37737:CDS |
| TACTTGTCTACACCATTCCA+TGG | 0.570760 | 6.3:+72068178 | MS.gene37737:CDS |
| TGAAAGACTGCACCACACTA+GGG | 0.577137 | 6.3:-72068343 | None:intergenic |
| TATGGTAAATGTAGAAGTAG+TGG | 0.581114 | 6.3:+72067996 | MS.gene37737:CDS |
| CAAGCTTCCACACCATGGAA+TGG | 0.584005 | 6.3:-72068190 | None:intergenic |
| TGAAGCTTCAAATGAACAAG+TGG | 0.587694 | 6.3:+72067893 | MS.gene37737:CDS |
| AAGTGGTTGTGTGCTCAAGA+AGG | 0.588756 | 6.3:-72067859 | None:intergenic |
| GTCTACACCATTCCATGGTG+TGG | 0.596900 | 6.3:+72068183 | MS.gene37737:CDS |
| ACCTGTGAATGGGTATAACC+GGG | 0.598899 | 6.3:-72067965 | None:intergenic |
| TTAATGTATCTAAGAGACAA+TGG | 0.603832 | 6.3:+72068219 | MS.gene37737:CDS |
| GTATGGTGATGCTTATAAGT+TGG | 0.604702 | 6.3:+72068313 | MS.gene37737:CDS |
| TCAACAACCACAACATCAAG+AGG | 0.610607 | 6.3:-72068056 | None:intergenic |
| TATAGTAATTAGCATCAGCT+CGG | 0.621190 | 6.3:-72067938 | None:intergenic |
| GTTATACCCATTCACAGGTG+TGG | 0.621916 | 6.3:+72067969 | MS.gene37737:CDS |
| ACGATCAAGCTTCCACACCA+TGG | 0.629046 | 6.3:-72068195 | None:intergenic |
| CAACAACCACAACATCAAGA+GGG | 0.678487 | 6.3:-72068055 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTGTTGAAATTTAACTTTAA+GGG | - | chr6.3:72068441-72068460 | None:intergenic | 15.0% |
| !! | AATCTTGAACCAATTATTAA+TGG | - | chr6.3:72068288-72068307 | None:intergenic | 20.0% |
| !!! | CTTGTTGAAATTTAACTTTA+AGG | - | chr6.3:72068442-72068461 | None:intergenic | 20.0% |
| !!! | TGTTGAAATTTAACTTTAAG+GGG | - | chr6.3:72068440-72068459 | None:intergenic | 20.0% |
| ! | AGACAATGGTTTATTACTAT+TGG | + | chr6.3:72068233-72068252 | MS.gene37737:CDS | 25.0% |
| ! | TGCTAATTACTATATTATCC+CGG | + | chr6.3:72067947-72067966 | MS.gene37737:CDS | 25.0% |
| ! | TTAATGTATCTAAGAGACAA+TGG | + | chr6.3:72068219-72068238 | MS.gene37737:CDS | 25.0% |
| CAATGGTTTATTACTATTGG+TGG | + | chr6.3:72068236-72068255 | MS.gene37737:CDS | 30.0% | |
| CTACTTCTACATTTACCATA+AGG | - | chr6.3:72067996-72068015 | None:intergenic | 30.0% | |
| GATGGGAAACCATTAATAAT+TGG | + | chr6.3:72068276-72068295 | MS.gene37737:CDS | 30.0% | |
| TATAGTAATTAGCATCAGCT+CGG | - | chr6.3:72067941-72067960 | None:intergenic | 30.0% | |
| TATGGTAAATGTAGAAGTAG+TGG | + | chr6.3:72067996-72068015 | MS.gene37737:CDS | 30.0% | |
| ! | AAGTTTTTCCAATACTTGCT+AGG | - | chr6.3:72068034-72068053 | None:intergenic | 30.0% |
| ! | GAGTGTTTGTTGATGAAAAT+GGG | + | chr6.3:72068387-72068406 | MS.gene37737:CDS | 30.0% |
| !! | AGTTTTTCCAATACTTGCTA+GGG | - | chr6.3:72068033-72068052 | None:intergenic | 30.0% |
| !!! | ACACCTTTTTTTGGATTAAC+GGG | - | chr6.3:72068113-72068132 | None:intergenic | 30.0% |
| !!! | GACACCTTTTTTTGGATTAA+CGG | - | chr6.3:72068114-72068133 | None:intergenic | 30.0% |
| AAGCATATGTGTAAGGATGT+TGG | + | chr6.3:72068365-72068384 | MS.gene37737:CDS | 35.0% | |
| ACACCCGTTAATCCAAAAAA+AGG | + | chr6.3:72068107-72068126 | MS.gene37737:CDS | 35.0% | |
| GTCTTTCAAGCATATGTGTA+AGG | + | chr6.3:72068358-72068377 | MS.gene37737:CDS | 35.0% | |
| TGAAAATGGGAATAAGCGTT+TGG | + | chr6.3:72068400-72068419 | MS.gene37737:CDS | 35.0% | |
| TGAAGCTTCAAATGAACAAG+TGG | + | chr6.3:72067893-72067912 | MS.gene37737:CDS | 35.0% | |
| TGGTTCAAGATTGAGAAGTA+TGG | + | chr6.3:72068296-72068315 | MS.gene37737:CDS | 35.0% | |
| TTATTAATGGTTTCCCATCC+CGG | - | chr6.3:72068275-72068294 | None:intergenic | 35.0% | |
| ! | GGAGTGTTTGTTGATGAAAA+TGG | + | chr6.3:72068386-72068405 | MS.gene37737:CDS | 35.0% |
| ! | GTATGGTGATGCTTATAAGT+TGG | + | chr6.3:72068313-72068332 | MS.gene37737:CDS | 35.0% |
| !! | ATTACTATTGGTGGTGTTGT+TGG | + | chr6.3:72068245-72068264 | MS.gene37737:CDS | 35.0% |
| !! | TTTTGGATTAACGGGTGTAA+AGG | - | chr6.3:72068105-72068124 | None:intergenic | 35.0% |
| !!! | ACACGAATGACACCTTTTTT+TGG | - | chr6.3:72068122-72068141 | None:intergenic | 35.0% |
| CAACAACCACAACATCAAGA+GGG | - | chr6.3:72068058-72068077 | None:intergenic | 40.0% | |
| GAACAAGTGGTTGACACATT+AGG | + | chr6.3:72067906-72067925 | MS.gene37737:CDS | 40.0% | |
| TACTTGTCTACACCATTCCA+TGG | + | chr6.3:72068178-72068197 | MS.gene37737:CDS | 40.0% | |
| TCAACAACCACAACATCAAG+AGG | - | chr6.3:72068059-72068078 | None:intergenic | 40.0% | |
| TGCTCAAGAAGGCAAAAAGA+AGG | - | chr6.3:72067851-72067870 | None:intergenic | 40.0% | |
| ! | TTAACGGGTGTAAAGGTTAG+TGG | - | chr6.3:72068098-72068117 | None:intergenic | 40.0% |
| AACTTGCCCTCTTGATGTTG+TGG | + | chr6.3:72068049-72068068 | MS.gene37737:CDS | 45.0% | |
| AAGTGGTTGTGTGCTCAAGA+AGG | - | chr6.3:72067862-72067881 | None:intergenic | 45.0% | |
| ACCTGTGAATGGGTATAACC+GGG | - | chr6.3:72067968-72067987 | None:intergenic | 45.0% | |
| AGTCTTGCCCTAGCAAGTAT+TGG | + | chr6.3:72068023-72068042 | MS.gene37737:CDS | 45.0% | |
| ATAAGGTCCACACCTGTGAA+TGG | - | chr6.3:72067979-72067998 | None:intergenic | 45.0% | |
| ATTCACAGGTGTGGACCTTA+TGG | + | chr6.3:72067978-72067997 | MS.gene37737:CDS | 45.0% | |
| CACCTGTGAATGGGTATAAC+CGG | - | chr6.3:72067969-72067988 | None:intergenic | 45.0% | |
| GTTATACCCATTCACAGGTG+TGG | + | chr6.3:72067969-72067988 | MS.gene37737:CDS | 45.0% | |
| TAAGGTCCACACCTGTGAAT+GGG | - | chr6.3:72067978-72067997 | None:intergenic | 45.0% | |
| TGAAAGACTGCACCACACTA+GGG | - | chr6.3:72068346-72068365 | None:intergenic | 45.0% | |
| TGTTGTTGGAAATCCGGGAT+GGG | + | chr6.3:72068259-72068278 | MS.gene37737:CDS | 45.0% | |
| TTGAAAGACTGCACCACACT+AGG | - | chr6.3:72068347-72068366 | None:intergenic | 45.0% | |
| TTGAGCACACAACCACTTCT+TGG | + | chr6.3:72067864-72067883 | MS.gene37737:CDS | 45.0% | |
| !! | TGGTGGTGTTGTTGGAAATC+CGG | + | chr6.3:72068253-72068272 | MS.gene37737:CDS | 45.0% |
| ACGATCAAGCTTCCACACCA+TGG | - | chr6.3:72068198-72068217 | None:intergenic | 50.0% | |
| CAAGCTTCCACACCATGGAA+TGG | - | chr6.3:72068193-72068212 | None:intergenic | 50.0% | |
| GTCTACACCATTCCATGGTG+TGG | + | chr6.3:72068183-72068202 | MS.gene37737:CDS | 50.0% | |
| TCCCGGTTATACCCATTCAC+AGG | + | chr6.3:72067964-72067983 | MS.gene37737:CDS | 50.0% | |
| ! | GTGTTGTTGGAAATCCGGGA+TGG | + | chr6.3:72068258-72068277 | MS.gene37737:CDS | 50.0% |
| !! | GGTGGTGTTGTTGGAAATCC+GGG | + | chr6.3:72068254-72068273 | MS.gene37737:CDS | 50.0% |
| !! | GTTGGTGTTTTGCCCTAGTG+TGG | + | chr6.3:72068331-72068350 | MS.gene37737:CDS | 50.0% |
| GCTTCAGCTGCTCCAAGAAG+TGG | - | chr6.3:72067879-72067898 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 72067822 | 72068466 | 72067822 | ID=MS.gene37737 |
| chr6.3 | mRNA | 72067822 | 72068466 | 72067822 | ID=MS.gene37737.t1;Parent=MS.gene37737 |
| chr6.3 | exon | 72067822 | 72068466 | 72067822 | ID=MS.gene37737.t1.exon1;Parent=MS.gene37737.t1 |
| chr6.3 | CDS | 72067822 | 72068466 | 72067822 | ID=cds.MS.gene37737.t1;Parent=MS.gene37737.t1 |
| Gene Sequence |
| Protein sequence |
